Publications

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[prevalence of parasitic diseases by toxocara canis in dogs, and the finding of eggs of toxocara species in the soil of public places in the urban area of londrina, state of parana, brazil]. 19761019565
[canine leishmaniasis in paraná state, southern brazil]. 19938209172
[evaluation of direct agglutination tests in the detection of leishmania (viannia) braziliensis in possible reservoirs of cutaneous american leishmaniasis in the state of ceará].in order to evaluate the aplicability of the direct agglutination test (dat) as a method of detection of the natural canine infection for leishmania (viannia) braziliensis, it was realized a study involving the serum of naive dogs from serra de baturite, ceara state, an endemic area of cutaneous leishmaniasis, and others from curitiba, parana state, a non endemic area. the results indicate the possibility of using this sorologic test to the epidemiologic research of the infection in the reservoi ...19968713609
[panstrongylus megistus in artificial ecotopes on islands of the paraná river].in response to complaints of triatomines on islands of the alto paraná river, an investigation to determine the presence of triatomines was conducted in 145 artificial ecotopes and 4 (2.8%) were infested, comprising a residence, a sports and leisure club, a former school and a woodpile. of the 35 p. megistus collected, 17 were analyzed and 12 (70.6%) were found to be infected with trypanosoma cruzi. birds and rodents were the most common food sources. all serologic examinations (56 human, 18 dog ...200111600918
prevalence of neospora caninum antibodies in dogs from dairy cattle farms in parana, brazil.serum samples from 134 dogs from 22 cattle dairy farms in the northern region of parana state, brazil, were tested for antibodies to neospora caninum in an indirect fluorescent antibody test. antibodies (> or = 1:50) to n. caninum were found in 29 (21.6%) of the 134 dogs, and seropositive dogs were found on 14 (63.6%) of the 22 dairy cattle farrms. the antibody titers of dogs were 1:50 (3 dogs), 1:100 (7 dogs), 1:200 (7 dogs), 1:400 (6 dogs), and > or = 1:800 (6 dogs). the low prevalence (9%) in ...200212054023
prevalence of neospora caninum antibodies and factors associated with their presence in dairy cattle of the north of paraná state, brazil.to determine the prevalence of neospora caninum antibodies and associated factors, blood sera from 623 female dairy cattle from 23 farms in the north of the state of paraná, brazil, were analyzed by means of the indirect immunofluorescent-antibody test (ifat > or = 25). serum samples from 134 dogs living on the same farms also were tested for n. caninum antibodies (ifat > or = 50), and the presence of dogs was associated with the prevalence of n. caninum antibodies in cattle. the overall seropre ...200415350656
eco-epidemiological survey of leishmania (viannia) braziliensis american cutaneous and mucocutaneous leishmaniasis in ribeira valley river, paraná state, brazil.leishmaniasis is endemic since last century in adrianópolis municipality, ribeira valley and is a serious public health. a study carried out during 1993-2003 on epidemiological surveys conducted in rural communities showed 339 new cases of cutaneous leishmaniasis (cl) detected from four municipalities (adrianópolis, cerro azul, doutor ulysses and rio branco do sul). a larger prevalence of cutaneous lesions was observed in rural workers (36%), women with domestic activities (18%), and younger stu ...200415652328
epidemiological aspects of filariosis in dogs on the coast of paraná state, brazil: with emphasis on dirofilaria immitis.the present study determined the prevalence and geographical distribution of dirofilaria immitis and other filariae, from dogs in littoral areas of paraná state, in brazil. this survey spanned eight months, between 1998 and 1999, and was also designed to compare the efficacy of different tests for diagnosis of heartworm infection in that area. blood samples were collected from 256 native-owned dogs distributed along the paraná coastal area. five diagnostic procedures were used: direct smear exam ...200415262005
triatomine infestation and vector-borne transmission of chagas disease in northwest and central parana, brazil.triatomine infestation, prevalence of t. cruzi antibodies in humans and domestic animals, and variables potentially associated with the presence of triatomines in a rural domiciliary unit (du) were investigated in nine municipalities and one district of parana, brazil, from june 1996 to february 2000. dus were defined as all houses and annexes on a given piece of property. blood samples from human volunteers, dogs, and cats were submitted to indirect immunofluorescence. an epidemiological form w ...200415486661
pcr in the investigation of canine american tegumentary leishmaniasis in northwestern paraná state, brazil.american tegumentary leishmaniasis (atl) was studied in 143 dogs in a rural area in the county of mariluz, northwestern paraná state, brazil, using direct parasite search, indirect immunofluorescence (iif), and polymerase chain reaction (pcr). thirty-nine dogs (27.3%) presented lesions suggestive of the disease, 5 (12.8%) of which were positive in direct parasite search and pcr (lesion), and of these 5, 4 were also positive by iif. of the 34 dogs with negative direct parasite search, 12 (35.3%) ...200616583101
[canine american cutaneous leishmaniasis in municipalities of northern paraná state, brazil].american cutaneous leishmaniasis (acl) is an endemic disease in northern paraná state, where it affects humans and dogs. this study aimed to verify the occurrence of the canine disease in acl endemic areas. from september 1999 to july 2002, dogs were investigated in 7 rural areas of 5 municipalities where autochthonous human cases of acl had been reported. parasitological and serological methods were used. fourteen of 67 dogs studied (20.9%) had lesions suggestive of acl, of which 3 (21.4%) were ...200616410884
[human and canine american cutaneous leishmaniasis in mariluz, paraná state, brazil].an outbreak of american cutaneous leishmaniasis was reported in 2002 in mariluz, northwestern paraná state, brazil. of 38 humans who were investigated, four had healed lesions, ten showed lesions in the healing process, and 24 had active lesions. of the 126 dogs, 20 (15.9%) presented suggestive lesions and 24 (19%) had positive serology. parasites isolated from two patients and three dogs were identified as leishmania (viannia) braziliensis serodeme i. the captured sand flies were identified as ...200617096049
species diversity and seasonality of free-living ticks (acari: ixodidae) in the natural habitat of wild marsh deer (blastocerus dichotomus) in southeastern brazil.this study evaluated the presence and seasonal activity of free-living ticks in remaining marsh areas by the paraná river, in brazil. eight field trips (once per season) for collection of ticks were performed during 2 years. using co2 traps, dragging, and visual inspection of vegetation, five free-living tick species were collected, in the following order of abundance: amblyomma cajennense, amblyomma dubitatum, amblyomma triste, amblyomma coelebs, and amblyomma nodosum. the seasonal pattern of a ...200716962241
giant kidney worm (dioctophyma renale) infections in dogs from northern paraná, brazil.this article describes the pathological observations of dioctophyma renale in dogs from the northern region of the state of paraná, brazil. a female, 6-year-old dog, fila brasileiro breed and a 16-year-old, male poodle were diagnosed positive for d. renale during routine necropsy. clinically, both dogs demonstrated hematuria, and the poodle had a radiographic diagnosis of prostatic tumor, but neither had a clinical diagnosis of this infection prior to necropsy. three giant worms were observed in ...200717156927
prevalence of neospora caninum and toxoplasma gondii in sheep and dogs from guarapuava farms, paraná state, brazil.sheep and dog blood samples were collected from nine farms in the county of guarapuava, paraná, brazil. the indirect fluorescent antibody test (ifat) was used to detect neospora caninum and toxoplasma gondii antibodies. herein, serum samples from 305 sheep were evaluated, being 29 (9.5%) and 157 (51.5%) seropositives to n. caninum and t. gondii, respectively. seven (29.1%) and five (20.8%) out of 24 dogs were seropositives to n. caninum and t. gondii, respectively. there were no differences amon ...200717266999
[preliminary study on feeding preference of nyssomyia neivai (pinto) and nyssomyia whitmani (antunes & coutinho) (diptera: psychodidae) in a rural area of the state of paraná, south brazil].due to the importance of sandflies in the american cutaneous leishmaniasis epidemiology, we investigated the feeding preference of sandflies related to domestic animals common in domiciliary environments in endemic rural areas, where this disease occurs. the sandflies were collected with falcão light traps, installed in cages, each one containing one domestic animal kind (pig, dog, rabbit or chicken), in recanto marista, in doutor camargo municipality. forty five collections were made, totaling ...200718246272
leishmania (viannia) braziliensis: epidemiology of canine cutaneous leishmaniasis in the state of paraná (brazil).the present study examines the role that dogs play in the maintenance of the leishmania cycle in the state of paraná, southern brazil. dogs were examined in three regions where cutaneous leishmaniasis is endemic or epidemic (r1-vale da ribeira; r2-central region of paraná state and r3-northern region). to determine serum prevalence rates elisa was used. in regions endemic for trypanosoma cruzi (r1 and r3), serum from dogs seroreactive towards leishmania antigen was subjected to t. cruzi adsorpti ...200717449032
survey of owned feline and canine populations in apartments from a neighbourhood in curitiba, brazil.animal population estimates are essential for public health services to ensure the success of zoonoses control programmes. canine and feline populations vary among different regions mainly because of local human income status and type of human residence. accordingly, the present study estimated the pet population size living in apartments of a vertical neighbourhood in curitiba, brazil. we chose a neighbourhood with a predominance of apartment buildings. all apartment buildings were visited, and ...200818811904
survey of giardiosis in household and shelter dogs from metropolitan areas of curitiba, paraná state, southern brazil.giardia duodenalis is a protozoan parasite that causes a broad range of clinical symptoms varying from none--in asymptomatic carriers--to mild recurring diarrhea consisting of soft, light-colored stools to acute severe diarrhea. in different parts of the world this parasite has raised increased interest due to its possible zoonotic transmission. among domestic animals, dogs can play an important role in environmental contamination. as there is little information on the frequency of giardiosis in ...200818261855
frequency of antibodies against neospora caninum in stray and domiciled dogs from urban, periurban and rural areas from paraná state, southern brazil.antibodies to neospora caninum were analyzed by an indirect fluorescent antibody test (ifat), in stray and domiciled dogs from urban, periurban and rural areas from curitiba, paraná state, brazil. of the total of 556 dogs, 18.17% (101) were positive for n. caninum (titer 50). frequency of antibody was 12.71 in urban, 15.73 in periurban, and 25.38% in rural dogs. rural dogs had a significantly higher frequency of antibodies than urban dogs (p<0.05). there were no significant differences between t ...200819265582
[determination and correlation of anti-neospora caninum antibodies in cattle and dogs from paraná, brazil].the protozoan parasite neospora caninum is a major cause of abortion in cattle worldwide. the aim of the present study was to determine and to compare the presence of anti-neospora caninum antibodies in cattle, as well as in farm dogs, and the relationship among them. the research was conducted in different mesoregions of paraná state, southern brazil. sera from 1.263 cattle, from 77 farms were tested for antibodies to n. caninum by elisa. sera from 129 dogs, from 35 farms were tested for antibo ...200820059847
[occurrence and molecular characterization of babesia species in a canine hospital population in the londrina region, parana state, brazil].canine babesiosis is a worldwide disease caused by the protozoan of babesia genus. babesia canis and b. gibsoni are both species that naturally infect dogs. the objective of this study was to evaluate the infection of babesia species in dogs attended at the londrina state university veterinary teaching hospital (hv-uel). it was selected 282 dogs seen at the londrina state university veterinary teaching hospital (hv-uel) between april of 2005 and may of 2006. they presented anemia (packed cell vo ...200820059862
[american cutaneous leishmaniasis in municipalities in the northwestern region of paraná state: use of remote sensing for analysis of vegetation types and places with disease occurrence].american cutaneous leishmaniasis, an endemic disease in the northwestern region of paraná, brazil, is transmitted by phlebotomines to man and animals like dogs, armadillos, opossums and wild rodents. this disease has been occurring in places where forests have been felled and on the banks of rivers and lakes with arboreal vegetation, where man comes into contact with infected insects. this disease is a public health problem because of the occurrence of epidemic outbreaks throughout paraná. becau ...200819142445
american cutaneous leishmaniasis in horses from endemic areas in the north-central mesoregion of paraná state, brazil.american cutaneous leishmaniasis (acl) is an endemic disease in the north-west of paraná state, brazil, where it occurs in humans and dogs. few studies have been undertaken on the occurrence of the disease in other domestic animals and horses. in this study we investigated the infection of horses by leishmania in acl-endemic rural areas. direct agglutination test (dat) and polymerase chain reaction (pcr) were employed. fifty-five horses from rural areas in the municipalities of doutor camargo, o ...200818331518
a new focus of cutaneous leishmaniasis in the central area of paraná state, southern brazil.we report a new endemic zone of cutaneous leishmaniasis (cl) in the central area of the state of paraná (municipality of prudentópolis), in southern brazil. this region was not previously considered endemic for cl, and this work constitutes the first report of cl endemicity there. leishmaniasis was confirmed by smear, culture, and elisa. parasites were isolated and identified by random amplification of polymorphic dna (pcr-rapd). phylogeographical analysis, based on two different criteria, was a ...200919482000
a serological and molecular investigation of american cutaneous leishmaniasis in dogs, three years after an outbreak in the northwest of paraná state, brazil.classic and molecular (polymerase chain reaction--pcr) techniques were used to diagnose american cutaneous leishmaniasis in 149 dogs from an area in the northwest of paraná state, brazil, where an american cutaneous leishmaniasis outbreak occurred in 2002. the results were compared to a set of previously obtained results. twenty-five dogs had positive indirect immunofluorescence (iif) (titers > or = 40), including two animals with suggestive lesions. the percentage of dogs with positive iif was ...200919180291
[allochthonous cases of canine visceral leishmaniasis in paraná, brazil: epidemiological implications].the south region is the only area in brazil that does not present autochthonous cases of visceral leishmaniasis (vl), however, in the state of paraná, dogs and humans have been found showing a vl compatible clinical profile. in view of this problem, the present work aimed at isolating and identifying the parasite and determining the cases autochthony. all animals clinically suspect of vl were clinically evaluated, and had samples of their blood collected for hemoculture (nnn culture medium), ser ...200919772775
seroprevalence of rickettsia bellii and rickettsia felis in dogs, são josé dos pinhais, state of paraná, brazil.brazilian spotted fever (bsf) is a vector-borne zoonosis caused by rickettsia rickettsii bacteria. dogs can be host sentinels for this bacterium. the aim of the study was to determine the presence of antibodies against rickettsia spp. in dogs from the city of são josé dos pinhais, state of paraná, southern brazil, where a human case of bsf was first reported in the state. between february 2006 and july 2007, serum samples from 364 dogs were collected and tested at 1:64 dilutions by indirect immu ...201021184698
serological survey of rickettsia sp. in horses and dogs in a non-endemic area in brazil.brazilian spotted fever (bsf) is a lethal rickettsiosis in humans caused by the bacteria rickettsia rickettsii, and is endemic in some areas of brazil. horses and dogs are part of the disease's life cycle and they may also serve as sentinel animals in epidemiological studies. the first human bsf case in the state of paraná was reported in 2005. the present study was conducted in the municipality of almirante tamandaré, where no previous case of bsf was reported. serum samples were collected from ...201021184695
lutzomyia longipalpis in uruguay: the first report and the potential of visceral leishmaniasis transmission.phlebotomine captures were performed in february 2010 in salto (salto department) and bella unión-cuarein (artigas department), uruguay. bella unión is located across the paraná river from monte caseros, argentina, where a focus of canine visceral leishmaniasis (vl) was reported in 2009. no vl cases have ever been recorded in uruguay and the last reported capture of phlebotominae was in 1932 (lutzomyia cortelezzii and lutzomyia gaminarai). light traps were placed in peridomestic environments, an ...201121655832
infection by spotted fever rickettsiae in people, dogs, horses and ticks in londrina, parana state, brazil.spotted fever is a disease caused by bacteria from the genus rickettsia of the spotted fever group (sfg). rickettsia rickettsii is likely the main agent of brazilian spotted fever (bsf). with the objective of gathering information on the circulation of sfg rickettsiae in londrina, parana state, ticks from dogs and horses and also blood from dogs, horses and humans were collected in a neighbourhood of the city which presented potential for circulation of rickettsiae between hosts and vectors. amb ...201121824336
ixodidae fauna of domestic dogs in parana, southern brazil.the present study aimed to contribute towards identification and registration of tick species that parasitize dogs in rural and urban areas of three mesoregions of paraná, southern brazil, and to estimate the rate of occurrence of each species. fifty-six dogs with ticks living in three mesoregions: metropolitana de curitiba (mc), centro oriental (cop) and centro sul paranaense (csp), were used in the study. from these 56 dogs, 253 ticks were collected and were identified and morphologically char ...201728678895
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