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microbiological and molecular determination of mycobiota in fresh and ensiled maize silage.the mycobiota of fresh and ensiled maize was studied with culturing techniques and a dna sequence-based approach. freshly chopped and ensiled maize were collected for 2 y from 12 farms in pennsylvania. samples were plated on selective media and isolates identified by morphology and sequences of the internal transcribed spacer regions of rdna, 800-900 bp of the 5' end of the translation elongation factor 1-alpha gene and a portion of the roda gene (aspergillus fumigatus only). its regions were am ...200717682779
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