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each water outlet is a unique ecological niche for legionella pneumophila.we determined the natural history of the colonization of our hospital's potable water by culturing water approximately biweekly from 20 sites throughout the hospital for 4 years. overall, 545 (24.7%) of the 2200 samples grew legionella pneumophila. during hyperchlorination, 11.7% of the samples were positive while 41.6% were positive in the absence of chlorination. there was no seasonal trend towards positivity, but there was marked inter-site variation in the semi-quantitative culture results. ...19921582468
characterization and cloning of a 37.6-kb plasmid carried by legionella pneumophila recovered from patients and hospital water over a 12-year period.for 12 years, strains of legionella pneumophila serogroup 1 harbouring a 37.6-kb (23 mda) plasmid have predominated among patient and potable water isolates at the victoria general hospital, halifax, n.s. plasmid dna recovered from 24 strains isolated between 1983 and 1995 was digested with the restriction endonucleases ecori, hindiii, kpni, pvuii, xbai, and bamhi. the distribution of cutting sites indicated that the 23-mda size group had remained essentially unchanged during this period, sugges ...19979090107
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