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evaluation of real-time nucleic acid sequence-based amplification for detection of chikungunya virus in clinical samples.the chikungunya virus (chikv) is a member of the genus alphavirus that is transmitted to humans by aedes mosquitoes. in 2005 and 2006, the indian ocean island of la réunion was hit with an unprecedented chikv fever outbreak that infected 300 000 people. in the present study, we describe the evaluation of real-time nucleic acid sequence-based amplification (rt-nasba) for the detection of chikv in clinical samples. a co-extracted and co-amplified chimerical chikv rna sequence was used as an intern ...200919528148
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