Publications

isolation of tick-borne encephalitis virus from ixodes ovatus (acari: ixodidae) in japan.	a case of tick-borne encephalitis (tbe) was found in a farming area located in the southern part of hokkaido, japan, in 1993. tbe viruses were isolated from sentinel dogs in the area where the human case occurred in 1995. ticks were collected in the area by 2 collection methods in 1995 and virus isolation was conducted on ticks in 1995 and 1996. ixodes ovatus neumann was found to be the predominant tick species in the area. in 1996, 2 virus strains were isolated from 600 i. ovatus ticks (300 fem ...	1998	9615539
epidemiology of tick-borne encephalitis in japan.	a tick-borne encephalitis (tbe) patient has not been reported for many years in japan although a serological survey of domestic animal sera suggested the presence of tbe foci in hokkaido, the northern island of japan. in october 1993 in hokkaido, a severe encephalitis case in a farm wife was diagnosed as tick-borne encephalitis. serological examination of paired sera showed a rise of neutralization (nt) antibody titer to russian spring summer encephalitis virus. a sero-epizootiological survey of ...	1998	9611678
phylogenetic and virulence analysis of tick-borne encephalitis viruses from japan and far-eastern russia.	we have previously reported that tick-borne encephalitis (tbe) is endemic in a specific area of hokkaido, japan. in oshima, the southern part of hokkaido, tbe virus was isolated from sentinel dogs, ticks and rodents in 1995 and 1996. to identify when these tbe viruses emerged in hokkaido, the times of divergence of tbe virus strains isolated in oshima and far-eastern russia were estimated. tbe virus was isolated in khabarovsk in 1998 and the nucleotide sequences of viral envelope protein genes o ...	1999	10567643
isolation of tick-borne encephalitis virus from wild rodents and a seroepizootiologic survey in hokkaido, japan.	to determine the vertebrate host of tick-borne encephalitis (tbe) virus in the southern part of hokkaido, japan, virus isolation was performed using spleens from small mammals captured in the area. two virus strains were isolated, one strain from apodemus speciosus and another from clethrionomys rufocanus. virus isolates were inoculated onto baby hamster kidney cell monolayers and antigen slides were prepared for an indirect immunofluorescent antibody assay. two isolates were identified as tbe v ...	1999	10072153
pathogenicity of tick-borne encephalitis virus isolated in hokkaido, japan in mouse model.	the pathogenic characteristics of tick-borne encephalitis (tbe) virus strain (oshima 5-10) isolated from a sentinel dog in hokkaido, japan, was compared by use of a mouse model with several inoculation routes to other strains of tbe virus (the far eastern subtype; sofjin strain and the western subtype; hochosterwitz strain) and tbe complex virus (langat virus; tp-21 strain). the degree of neuroinvasiveness of the strains in mice subcutaneously (s.c.) inoculated was sofjin equaled hochosterwitz w ...	1999	10067683
characterization of monoclonal antibodies against hokkaido strain tick-borne encephalitis virus.	a tick-borne encephalitis (tbe) patient was found in hokkaido in 1993, and tbe viruses were isolated from animals and ticks in our previous studies. to develop a diagnostic reagent to identify tbe viruses, monoclonal antibodies (mabs) were produced against the tbe virus strain hokkaido (oshima 5-10). seven mabs were obtained which reacted with the envelope protein of the oshima 5-10 strain. these mabs were flavivirus genus-specific, tbe virus complex-specific or tbe virus type-specific. the mabs ...	2000	10941938
epidemiology of tick-borne encephalitis (tbe) and phylogenetic analysis of tbe viruses in japan and far eastern russia.	in oshima, the southern part of hokkaido, a tick-borne encephalitis (tbe) patient was found in 1993; in addition tbe virus was isolated from the blood samples of sentinel dogs, ticks pools, and rodents spleens in 1995 and 1996 by suckling mice. to identify when these tbe viruses emerged in hokkaido, the times of divergence of tbe virus strains isolated in oshima and far eastern russia were estimated. tbe virus was isolated in khabarovsk in 1998, and the nucleotide sequences of viral envelope pro ...	2001	11326122
genetic and biological comparison of tick-borne encephalitis viruses from hokkaido and far-eastern russia.	we compared the biological properties of oshima 5-10 (tick-borne encephalitis [tbe] virus isolated in hokkaido, japan) and sofjin-ho (far-eastern subtype tbe virus) including plaque formation, virus replication and virus protein synthesis in bhk-21 cell cultures to reveal strain differences. we also determined the complete nucleotide sequences of both strains and compared the deduced amino acid sequences. plaques of oshima 5-10 were smaller than those of sofjin-ho. virus titers in culture fluid ...	2002	11949477
[phylogenetic analysis and pathogenicity of tick-borne encephalitis virus from japan and far-east russia].	phylogenetic analysis of tick-borne encephalitis (tbe) virus revealed that hokkaido strain of tbe virus evolved several hundreds years ago in far-east russia. tbe virus strains in irkutsk area were identified as siberian subtype of tbe virus. bhk-cell adapted mutant of tbe virus showed lower neuro-invasive virulence in mice than parent virus. the mutant carried one amino acid substitution in envelope protein which resulted in increase of positive charge of the protein. the mutant-infected mice s ...	2005	16308528
epizootiological study of tick-borne encephalitis virus infection in japan.	tick-borne encephalitis virus (tbev) is a zoonotic agent causing severe encephalitis in humans. rodent species that are potential hosts for tbev are widely distributed in various regions in japan. in this study, we carried out large-scale epizootiological surveys in rodents from various areas of japan. a total of 931 rodent and insectivore sera were collected from field surveys. rodents seropositive for tbev were found in shimane prefecture in honshu and in several areas of hokkaido prefecture. ...	2010	21060247
genetic and biological characterization of tick-borne encephalitis virus isolated from wild rodents in southern hokkaido, japan in 2008.	tick-borne encephalitis virus (tbev) is a zoonotic agent causing severe encephalitis in humans. a recent epizootiological survey indicated that endemic foci of tbev have been maintained in the southern part of hokkaido until recently. in this study, we sought to isolate tbev from wild rodents in the area. one virus, designated oshima 08-as, was isolated from an apodemus speciosus captured in hokuto in 2008. oshima 08-as was classified as the far eastern subtype of tbev and formed a cluster with ...	2013	23590320