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distribution and sequence analysis of virulence associated genes in vibrio cholerae o1, o139 and non-o1/non-o139 isolates from thailand.virulence-associated genes of vibrio cholerae including o1, o139 and non-o1/non-o139 from an outbreak in songkhla province and sporadic cases occurred in thailand during 1993 - 2002 were investigated. one hundred eighty-five v. cholerae strains were examined for the presence of virulence-associated genes including ctxa, tcpa, zot, toxr, toxs, toxt, and ace by polymerase chain reaction. dna sequences of ctxa, tcpa, zot and toxr also were investigated in 8 selected isolates. results showed that th ...200919842384
pulsed field gel electrophoresis analysis of vibrio cholerae isolates in southern thailand.forty isolates of v. cholorae o1, o139 and non-o1/non-o139 collected from outbreaks in songkhla and phuket provinces of southern thailand during 1999-2001 and sporadic cases from different regions of thailand during 1993-2002 were characterized using pulsed field gel electrophoresis (pfge). digestion of chromosomal dna of the v cholerae isolates with restriction endonuclease noti, followed by pfge, generated 10 distinct restriction endonuclease analysis patterns consisting of 8 to 13 bands, rang ...201020578525
isolation and characterization of vibrio cholerae isolates from seafood in hat yai city, songkhla, thailand.seafood has been identified as an important source of vibrio cholerae in thailand, especially in the southern coastal region. in this study, we isolated and characterized v. cholerae from seafood obtained from several markets in hat yai city, southern thailand. a total of 100 v. cholerae isolates were obtained from 55 of 125 seafood samples. the dominant serotype was non-o1/non-o139. polymerase chain reaction (pcr) analysis was used to detect the presence of pathogenesis-related genes. the stn/s ...201425188839
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