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characteristics of crimean-congo hemorrhagic fever virus (xinjiang strain) in china.virus strains isolated from blood of patients during a hemorrhagic fever outbreak in 1968 in southern xinjiang, china, from hyalomma asiaticum and from sheep, were found to be identical or closely related to crimean-congo hemorrhagic fever (c-chf) virus by complement fixation and indirect immunofluorescence tests with convalescent sera of patients and with c-chf reference antibody. the virus was inactivated by ether and acid. viral synthesis was not suppressed by 5-iododeoxyuridine suggesting an ...19852422968
nutrition, metabolism, and hypertension. a comparative survey between dietary variables and blood pressure among three nationalities in china.the prevalence of hypertension among kazak, han, and uygur nationalities living in xinjiang autonomous region was 15.3%, 4.2%, and 2.1%, respectively; 257 men (92, 92, 83 subjects, respectively), aged 40-59 years were studied. the variables analyzed were serum total cholesterol, total protein, albumin, alpha-gt, triglyceride, plasma fibrinogen, and glucose concentrations; and urinary na, k, ca, mg, urea nitrogen, taurine, sulfate, and nacns (an index of smoking), content. the data on nutritional ...19863760914
cystic echinococcosis in the xinjiang/uygur autonomous region, people's republic of china. i. demographic and epidemiologic data.demographic and epidemiologic data on those factors potentially related to the prevalence and transmission of hydatid disease caused by echinococcus granulosus were collected in 1987 from each household in 85 villages in the two communities of yuanhucun and ershilidian within hutubi county in the xinjiang/uygur autonomous region, prc, with additional questionnaire data collected from approximately 40 households randomly selected from each of 16 villages within those communities. yuanhucun and er ...19902382094
frequency distribution of echinococcus granulosus hydatid cysts in sheep populations in the xinjiang uygur autonomous region, china.age-prevalence and age-intensity data of echinococcus granulosus hydatid cysts in sheep populations were collected in an abattoir in the xinjiang uygur autonomous region, people's republic of china. the frequency distribution of the larval cysts per sheep was empirically described by the negative binomial model, with parameter k being 0.5273. a mathematical model for the life cycle of e. granulosus was applied to the collected data and the results show that the infection pressure on sheep was 0. ...19921441193
treatment and control of the intestinal fluke, skrjabinotrema ovis.the small intestinal fluke, skrjabinotrema ovis, is common among sheep in the xinjiang region of western china. up to 65,000 flukes per sheep were found during september when sheep were grazing summer pastures at high altitudes. efficacy studies of some common flukicides revealed that oxyclosanide and triclabendazole were not effective in reducing the egg output of s. ovis, whereas closantel and praziquantel reduced egg counts by up to 98%. praziquantel at 25 and 50 mg kg-1 reduced worm counts b ...19938128588
[rflp analysis of dna from echinococcus granulosus collected from four provinces/autonomous region in china].a fragment of the ribosomal rna gene of schistosoma mansoni psm889 and two dna fragments specific to echinococcus granulosus phd5 and peg18 have been used as dna probes to assess the extent of genetic variability within e. granulosus. the dna analysis, including restriction endonuclease digestion and southern blot hybridization with the probes, did not demonstrate any genetic variation among e. granulosus collected from sheep in xinjiang, qinghai, gansu and ninxia. similarly, there was no geneti ...19937909504
epidemiological studies on cystic echinococcosis in china--a review.in the four decades from 1951 to 1990, the six provinces or autonomous regions (xinjiang, gansu, qinghai, ningxia, xizang and nei monggol) reported a total of 26,065 surgical cases of hydatid disease, most of which were reported in the recent decade. about one third of the patients was children and adolescents under 15 years old. so far, cystic hydatid infections of local origin have been confirmed in 22 provinces, autonomous regions and municipalities in the whole nation. findings of x-ray exam ...19957546341
the efficacy of an albendazole-medicated block in controlling sheep nematodes in xinjiang province, north-west china.medicated feed-blocks containing 2 mg/kg albendazole were fed for 12 days to ewe-lambs naturally infected with nematode worms in north-west china. the cumulative total average intake of albendazole was 15 mg/kg body weight. this treatment was compared with a single oral drench of 15 mg/kg albendazole and with no treatment. both albendazole treatments were 99% effective in reducing the total strongyle egg counts. the medicated block was 100% effective in reducing the total trichostrongylid worm c ...19968908723
dynamics of environmental supplementation of iodine: four years' experience of iodination of irrigation water in hotien, xinjiang, china.hotien prefecture, xinjiang province, china, in the taklamakan desert, is an area of severe iodine deficiency. because usual methods of iodine supplementation failed here, we began supplementation in 1992 with potassium iodate, which was added to irrigation water (lancet 1994; 334:107-110). we report 4 y experience with this method in 3 townships that contained a total treated population of 37,000. potassium iodate was dripped into irrigation water (to a concentration 10-80 microg/l) during a 2- ...19979541360
mitochondrial genomic markers confirm the presence of the camel strain (g6 genotype) of echinococcus granulosus in north-western china.twenty-eight isolates of e. granulosus, collected from humans at surgery, and a range of intermediate hosts, including sheep, cattle and camels from abattoirs in north and south xinjiang uygur autonomous region, people's republic of china, were analysed for dna sequence variation within regions of the mitochondrial cytochrome c oxidase i (coi) and nadh dehydrogenase subunit i (ndi) genes. the isolates were categorized into 2 distinct and uniform genotypic groupings, based on the sequences obtain ...19989481771
seroprevalence of borna disease virus in domestic animals in xinjiang, china.to investigate the animals infected with borna disease virus (bdv) in xinjiang, china, we examined for bdv antibodies in the sera from groups of 20 horses, sheep and cattle, and from 165 wild rodents (18 species) by elisa and immunoblot. the serological study disclosed the presence of antibodies to both bdv-p24 and -p40 in the horses (20%) and sheep (25%), whereas no apparent positive reaction was detected either in cattle or rodents. the results suggested that bdv is prevalent in horses and she ...200111348775
[serologic studies of xinjiang hemorrhagic fever in bachu county, 2001].to investigate the situation of xinjiang hemorrhagic fever (xhf) in patients who have been diagnosed as xhf by clinical methods and to predict the condition in people who were liable to infection and in the host-animals.200212411084
[microsatellite analysis of genetic diversity and phylogenetic relationship of eight sheep breeds in xinjiang].to reveal the genetic diversity and syseighttemic relationship of main sheep breeds in north xingjiang, the genetic polymorphisms of 10 microsatellites in 8 sheep breeds and one first filial generation (f1) in north xinjiang were studied by means of pcr, polyacrylamide gel electrophoresis and silver staining. number of alleles, average effective number of alleles (e) and average rates of homozygote of each breeds were counted. according to allele frequencies of ten microsatellites, polymorphism ...200314577377
detection of immunoglobulin g to crimean-congo hemorrhagic fever virus in sheep sera by recombinant nucleoprotein-based enzyme-linked immunosorbent and immunofluorescence assays.crimean-congo hemorrhagic fever virus is a tick-borne virus that causes severe hemorrhagic symptoms with an up to 50% mortality rate in humans. wild and domestic animals, such as sheep, cattle and goats, are the reservoirs. the recombinant nucleoprotein-based crimean-congo hemorrhagic fever virus antibody detection systems for sheep sera were developed by enzyme-linked immunosorbent assay (elisa) and an indirect immunofluorescence assay techniques. the samples used for evaluation were 80 sera co ...200312565161
cloning, homological analysis and construction of eg95 xinjiang strain dna vaccine.to study the structure specificity of echinococcus granulosus 95 (eg95) gene and the open reading frame (orf) of the full-length cdna sequence in xinjiang, northwestern china and construct eg95 xinjiang strain dna vaccine.200314627516
short report: echinococcus granulosus from xinjiang, pr china: cdnas encoding the eg95 vaccine antigen are expressed in different life cycle stages and are conserved in the oncosphere.the eg95-based vaccine protects sheep from infection with the dog tapeworm echinococcus granulosus. the eg95 encoding gene is a member of a multigene family, several members of which are expressed in the oncosphere, believed to be the target of immunity induced by the vaccine. e. granulosus exhibits extensive intraspecific (strain) variation, and variability of the eg95 gene in different isolates of e. granulosus may directly impact the effectiveness of the eg95-based vaccine. we analyzed the eg ...200312556145
epidemiological evaluations of the efficacy of slow-released praziquantel-medicated bars for dogs in the prevention and control of cystic echinococcosis in man and animals.to assess the epidemiological efficacy of type srp iii slow-released praziquantel-medicated bars for dogs in the prevention and control of cystic echinococcosis in man and livestock, praziquantel-medicated bars were implanted subcutaneously in over 90% of dogs in villages in north xinjiang, china, where cystic echinococcosis is highly endemic. after implantation, infection rate of echinococcus granulosus in dogs, specific antibodies in children and prevalence of echinococcosis in one-year-old la ...200516231860
[geography and host distribution of crimean-congo hemorrhagic fever in the tarim basin].to determine the infective status and natural distribution of xinjiang hemorrhagic fever (xhf; crimean-congo hemorrhagic fever, cchf) in ticks, rodents and livestock in the tarim basin.200617415983
[polymorphism analysis of genes associated with hindquarters muscular development on chromosome 18 in xinjiang meat sheep].through polymorphism analysis of genes associated with hindquarters development on chromosome 18 in xinjiang meat sheep, we sought genes that are associated with increased hindquarters musculature in the hope to provide theoretical guidance to molecular marker-assisted selection of meat sheep. the polymorphism of callipyge (clpg) gene was analyzed by pcr-sscp and pcr-rflp in populations of dorset and suffolk breeds and their f1 and f2 crosses with local xinjiang fine wool sheep. results showed t ...200616825168
prion protein gene (prnp) polymorphisms in xinjiang local sheep breeds in china.amino acid polymorphisms of the prion protein (prp) gene (prnp), particularly those occurring at codons 136, 154, and 171 have a significant influence on scrapie pathogenesis in many sheep breeds. we isolated blood samples from 222 sheep representing the eight main local sheep breeds in the xinjiang autonomous region, the territory with the most abundant local sheep breeds in china, to identify the prnp polymorphisms and to determine whether these breeds were at risk for developing scrapie. a ne ...200616622593
morphological and molecular characteristics of echinococcus multilocularis and echinococcus granulosus mixed infection in a dog from xinjiang, china.the xinjiang plateau of western china has been shown to have a high prevalence for human cystic echinococcosis (ce) caused by echinococcus granulosus, and human alveolar echinococcosis (ae) caused by echinococcus multilocularis. the domestic dog is suspected to be the primary definitive host for the transmission of both e. granulosus and e. multilocularis to humans in this locality. seventeen of 30 stray dogs from hejing county of xinjiang were found positive for e. granulosus post mortem, and o ...200616616990
at least two genetically distinct large babesia species infective to sheep and goats in china.a fatal disease of sheep and goats in the northern part of china has been reported to be due to babesia ovis. however, some characteristics of the causative agent in recent reports are not in accordance with the original attributes ascribed to this parasite. therefore, the 18s small subunit ribosomal rna (18s rrna) genes of a number of babesia isolates in china were sequenced and compared with that of other babesia and theileria species in an attempt to clarify their taxonomic position. in the p ...200717531391
the development and evaluation of a loop-mediated isothermal amplification (lamp) method for detection of babesia spp. infective to sheep and goats in china.the loop-mediated isothermal amplification (lamp) reaction is a method that amplifies with high sensitivity, efficiency, and rapidity, deoxyribonucleic acid (dna) under isothermal condition in simple incubators. two primer sets for the lamp method were designed using the nucleotide sequences of 18s rrna gene of babesia sp. bq1 (lintan) and babesia sp. xinjiang-2005 isolated in china. the primers were used to detect parasite dna extracted from infected blood and purified parasites by lamp. the sp ...200818504039
a pilot study for control of hyperendemic cystic hydatid disease in china.cystic hydatid disease (chd) is a global parasitic zoonosis caused by the dog tapeworm, echinococcus granulosus. the disease is hyperendemic in western china because of poor economic development; limited community knowledge of chd; widespread, small-scale household animal production; home killing of livestock; and the feeding of dogs with uncooked offal.200919859535
a review of research progress of fecb gene in chinese breeds of sheep.fecb gene is a major gene responsible for high prolificacy firstly identified in booroola merino sheep. subsequently, many other aspects of the fecb including endocrinology, fetal and postnatal growth were studied. a forced pcr-rflp method was performed to screen some chinese breeds or strains of sheep to determine if fecb gene is responsible for their high prolificacies. the fecb gene was present in some chinese prolific breeds of sheep, such as huyang, small tail han (sth), cele, duolang sheep ...200919201555
[detection and sequences analysis of sheep hepatitis e virus rna in xinjiang autonomous region].to understand whether hepatitis e virus (hev) was infectious in sheep in xinjiang.201020815242
[polymorphism at codons 136, 141 and 154 in the ovine prion protein gene in the state of xinjiang].scrapie is a fatal and infectious neurodegenerating disease. the polymorphism in the prion protein (prnp) gene is linked to the development of clinical signs of scrapie. the most important polymorphism appears to be at codons at 136(v/a), 154(h/r), and 171(h/q/r). in this study, we investigated the polymorphisms at these codons in 746 individuals among ten sheep breeds (i.e., aletai, bashibai, bayinbuluke, celehei, duolang, he tian, chinese merino, german merino, texel, and suffolk sheep) in xin ...201021513168
[seroepidemiological survey of sheep hepatitis e virus infection in aksu region of xinjiang autonomous].four hundreds and ninety sheep sera from seven breeds raised at eight counties and one city of aksu region in xinjiang were tested by elisa for the presence of antibodies against hepatitis e virus (hev), and the positive rate differences were compared based on administrative areas, breeds and age by chi-square test. the result showed that the general positive rate was 28.98% (142/490), the positive rate were 35.44% (28/79), 29.67% (27/91), 20% (4/20), 40% (12/30), 32.5% (26/80), 38% (19/50), 22. ...201020572346
diversity and abundance of the rumen and fecal methanogens in altay sheep native to xinjiang and the influence of diversity on methane emissions.this study aims to investigate the influence of diet roughage proportion on the methanogenic communities from the rumen and fecal samples in altay local sheep native to xinjiang and better understand the association of methanogenic diversity or abundance with methane emissions of the ruminants. in this study, the high roughage diet was found to cause more methane emissions for either maintenance or ad-lib group, but the total methanogenic abundance was not influenced by roughage proportion and s ...201122038025
radiocarbon-dated archaeological record of early first millennium b.c. mounted pastoralists in the kunlun mountains, china.pastoral nomadism, as a successful economic and social system drawing on mobile herding, long-distance trade, and cavalry warfare, affected all polities of the eurasian continent. the role that arid inner asia, particularly the areas of northwestern china, kazakhstan, and mongolia, played in the emergence of this phenomenon remains a fundamental and still challenging question in prehistoric archaeology of the eurasian steppes. the cemetery of liushiu (xinjiang, china) reveals burial features, br ...201121911387
theileria ovis discovered in china.a polymerase chain reaction (pcr) using 989/990 primers was conducted to identify a newly isolated theileria sp. in xinjiang province of china. the target dna fragments of the complete 18s rrna gene were cloned and sequenced. the phylogenetic relationship of newly isolated theileria spp. was inferred based on the 18s rrna gene. the results showed that the new theileria sp. belonged to the cluster of theileria ovis. moreover, the findings were confirmed by t. ovis species-specific pcr. an expecte ...201120619262
[prevalence of anaplasma spp. infection in a desert landscape region of heshuo, xinjiang].to study the prevalence of anaplasmosis among human, domestic sheep and tick population in heshuo area, southern xinjiang and to investigate the diversity of anaplasma species.201323751470
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