Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| infectious diseases and governance of global risks through public communication and participation. | in recent years a succession of health emergencies connected with the threat of new, possibly global, infectious diseases has stimulated the attention of the mass media, the scientific community, and international public opinion, setting a tough test for the institutions whose job is to manage the risks. on the basis of experience in the fields of aids, bse, sars and bird flu, this study discusses the strong and weak points of governance procedures for health risks. in particular, the paper illu ... | 2007 | 18209267 |
| simulation-based estimation of bse infection in japan. | the first case of bovine spongiform encephalopathy (bse) in japan was found in september 2001. as a result, national bse surveillance systems in slaughterhouses and farms were introduced between october 2001 and april 2004. all cattle, with the exception of those under 24 months of age that die at farms, now undergo compulsory testing when they die or are slaughtered. the removal of specified risk material (srm) from all slaughtered cattle and a ban on the feeding of meat-and-bone meal to all fa ... | 2008 | 18192042 |
| review on the epidemiology and dynamics of bse epidemics. | the paper describes how the comprehensive surveillance of bovine spongiform encephalopathy (bse) and studies carried out on these data has enhanced our knowledge of the epidemiology of bse. around 7, 000 bse cases were detected through the screening of about 50 million cattle with rapid tests in europe. it confirmed that the clinical surveillance had a poor capacity to detect cases, and also showed the discrepancy of this passive surveillance efficiency between regions and production types (dair ... | 2008 | 18187031 |
| short communication: allele, genotype, and haplotype data for bovine spongiform encephalopathy-resistance polymorphisms from healthy us holstein cattle. | bovine spongiform encephalopathy (bse) is a neurodegenerative disease of cattle caused by abnormally folded prion proteins. two regulatory region polymorphisms in the bovine prion gene are associated with resistance to classical bse disease: a 23-bp region in the promoter that contains a binding site for the repressor protein rp58, and a 12-bp region in intron 1 that has a binding site for the transcription factor sp1. the presence of these binding sites enhances bse resistance in cattle, wherea ... | 2008 | 18096956 |
| bone regenerated via rhpdgf-bb and a deproteinized bovine bone matrix: backscattered electron microscopic element analysis. | this study used backscattered electron microscopy (bse-sem) to analyze specimens in which bone was augmented both horizontally and vertically with a xenograft scaffold and recombinant human platelet-derived growth factor (rhpdgf-bb), with or without a resorbable collagen membrane. the study objective was to compare percentage weight and volume calcium-phosphorus ratios of regenerated bone and native bone and the nature of the bony contact with two different implant surfaces. examination of the n ... | 2007 | 18092448 |
| comparison of the historic recycling risk for bse in three european countries by calculating the basic reproduction ratio r0. | a deterministic model of bse transmission is used to calculate the r(0) values for specific years of the bse epidemics in the united kingdom (uk), the netherlands (nl), and switzerland (ch). in all three countries, the r(0) values decreased below 1 after the introduction of a ban on feeding meat and bone meal (mbm) to ruminants around the 1990s. a variety of additional measures against bse led to further decrease of r(0) to about 0.06 in the years around 1998. the calculated r(0) values were con ... | 2007 | 18076489 |
| analyzing bse surveillance in low prevalence countries. | if the prevalence of bovine spongiform encephalopathy (bse) varies among cohorts within a population, stratified analysis of bse surveillance data may allow identification of differences in bse exposure that are important with respect to the design and evaluation of disease prevention and control measures. in low bse prevalence populations, however, surveillance at levels that meet or exceed international guidelines may provide insufficient statistical power to distinguish prevalence levels amon ... | 2008 | 17980447 |
| epidemiological study of the decline of bse in italy. | in this paper, data derived from the national database of the italian surveillance system for bovine spongiform encephalopathy (bse) are used to describe the italian epidemic of bse. two data flows were established to collect the results of active and passive surveillance, and 25 regional laboratories were involved. the national reference centre (cea) was in charge of the data analysis. crude and age-standardised estimates of the prevalence and incidence of bse were obtained to describe the dist ... | 2007 | 17938408 |
| mechanical stress and formation of protein aggregates in neurodegenerative disorders. | misfolded protein aggregates and inclusion bodies have been associated with various protein conformation disorders such as alzheimer's disease (ad), parkinson's disease (pd), amyotrophic lateral sclerosis (als), and prion diseases including bovine spongiform encephalopathy (bse). models have been proposed as plausible explanations for the extension and progression of protein aggregates; however, little is known about the initiation process of protein aggregation, particularly in sporadic neurode ... | 2008 | 17910993 |
| central nervous system tissue in meat products: an evaluation of risk, prevention strategies, and testing procedures. | since the outbreak of bovine spongiform encephalopathy (bse) in the united kingdom in 1986 and its subsequent link to the human neurological disorder variant creutzfeldt-jakob disease (vcjd), presence of tissues from the central nervous system (cns) in meat products has been considered a public health concern and, thus, has been banned from entering the human food chain in many countries. despite this, potential can exist during harvesting to contaminate or cross-contaminate edible meat products ... | 2007 | 17900496 |
| prion protein and the transmissible spongiform encephalopathies. | transmissible spongiform encephalopathies (tses) are fatal neurodegenerative diseases that occur in a wide variety of mammals. in humans, tse diseases include kuru, sporadic and iatrogenic creutzfeldt-jakob disease (cjd), gerstmann-sträussler-scheinker syndrome (gss), and fatal familial insomnia (ffi). so far, tse diseases occur only rarely in humans; however, scrapie is a widespread problem in sheep, and the recent epidemic of bovine spongiform encephalopathy (bse or mad cow disease) has seriou ... | 1997 | 17708907 |
| estimating the prevalence of bse in dairy birth cohorts and predicting the incidence of bse cases in japan. | following the detection of the first case of bse in japan in september 2001, four million cattle were subjected to a rapid test for bse up to the end of 2004. a further 10 cases were detected in the dairy cattle population and two cases in holstein steers. we focused on the dairy population and estimated the prevalence of bse infected animals within each birth cohort for the years 1992-2001 using bayesian inference. from this we were able to predict historic and future trends in the number of in ... | 2007 | 17617479 |
| targeted surveillance to assess the presence of bse in the age risk population of cattle slaughtered in bursa, turkey: preliminary results of an immunohistochemical detection study for the 2004-2005 period. | bovine spongiform encephalopathy (bse), a member of the transmissible spongiform encepahlopathies, has been a notifiable disease in turkey since 1997. in 2002, the bse status of turkey was assessed by the eu scientific steering committee as "it is likely but not confirmed". this study presents the results of a targeted surveillance study to assess the presence of bse in the age risk population of bursa, turkey. in the assessment procedure, the immunohistochemical detection of protease-resistant ... | 2007 | 17519575 |
| detection of central nervous system tissues in meat products: validation and standardization of a real-time pcr-based detection system. | several phenotypic as well as genotypic methods have been published describing the detection of central nervous system (cns) tissues that are part of the bovine spongiform encephalopathy (bse) risk material in food products. however, none of these methods is able to differentiate between cns tissue of the banned ruminant species and tissues of other animal species. a quantitative and species-specific real-time rt-pcr method has been developed that enables the reliable identification of cns tissu ... | 2007 | 17507185 |
| a model (bsurve) for estimating the prevalence of bovine spongiform encephalopathy in a national herd. | we developed the bsurve spreadsheet model to estimate the true prevalence of bovine spongiform encephalopathy (bse) in a national cattle population, and evaluate national bse surveillance programs. bsurve uses bse surveillance data and demographic information about the national cattle population. the proportion of each cohort infected with bse is found by equating the observed number of infected animals with the number expected, following a series of probability calculations and assuming a binom ... | 2007 | 17507106 |
| imported and indigenous bse cases in germany. | bovine spongiform encephalopathy (bse) is a transmissible fatal neurodegenerative disease in cattle with an average incubation time of five years. the first bse case in an indigenous cow was detected in germany in november 2000. this was almost eight years after the huge bse epidemic in the united kingdom had peaked, and several years after many eu member states had seen their first bse case. in the 1990s, bse had been diagnosed in six imported animals in germany. however, after the implementati ... | 2007 | 17493772 |
| reduced susceptibility to bovine spongiform encephalopathy prions in transgenic mice expressing a bovine prp with five octapeptide repeats. | in this work, transgenic (tg) mice were generated expressing a bovine prion protein containing five octarepeats (boprp(5or)-tg). after intracerebral inoculation of bovine spongiform encephalopathy (bse) inoculum, these mice suffered a bse-like neuropathology but survived longer compared with homologous tg mice expressing similar levels of a six octarepeat boprp protein (boprp(6or)-tg). de novo-generated five octarepeat (5or) prp(sc) showed no biochemical differences from 6or-prp(sc), and the pro ... | 2007 | 17485546 |
| atypical scrapie in a swiss goat and implications for transmissible spongiform encephalopathy surveillance. | different types of transmissible spongiform encephalopathies (tses) affect sheep and goats. in addition to the classical form of scrapie, both species are susceptible to experimental infections with the bovine spongiform encephalopathy (bse) agent, and in recent years atypical scrapie cases have been reported in sheep from different european countries. atypical scrapie in sheep is characterized by distinct histopathologic lesions and molecular characteristics of the abnormal scrapie prion protei ... | 2007 | 17459826 |
| molecular discrimination of atypical bovine spongiform encephalopathy strains from a geographical region spanning a wide area in europe. | transmissible spongiform encephalopathy strains can be differentiated by their behavior in bioassays and by molecular analyses of the disease-associated prion protein (prp) in a posttranslationally transformed conformation (prpsc). until recently, isolates from cases of bovine spongiform encephalopathy (bse) appeared to be very homogeneous. however, a limited number of atypical bse isolates have recently been identified upon analyses of the disease-associated proteinase k (pk) resistance-associa ... | 2007 | 17442800 |
| genotype distribution of the prion protein gene (prnp) promoter polymorphisms in korean cattle. | recently, an association between bovine spongiform encephalopathy (bse) and insertion/deletion (indel) polymorphisms in the bovine prion protein gene (prnp) promoter region has been reported in german cattle. these prnp polymorphisms cause changes in prnp expression and are thought to play an important role in bse susceptibility. bse has been reported in british and japanese holstein cattle but has not been diagnosed in hanwoo cattle (bos taurus coreanae) up to now. these results prompted us to ... | 2006 | 17426768 |
| lesion profiles and gliosis in the brainstem of 135 swiss cows with bovine spongiform encephalopathy (bse). | lesion profiles are considered to be an important tool for the comparison of the various animal and human spongiform encephalopathies and to obtain information upon prion strain variations. histological and immunohistochemical reactions (prpsc, gfap) in 13 brain areas at 4 levels in the brainstem from 135 bse-positive and 45 bse-negative cases were retrospectively evaluated. in this retrospective study a lesion profile based on histological features was worked out on the basis of bse cases origi ... | 2007 | 17410969 |
| bovine spongiform encephalopathy: the effect of oral exposure dose on attack rate and incubation period in cattle. | the dose-response of cattle exposed to the bovine spongiform encephalopathy (bse) agent is an important component of modelling exposure risks for animals and humans and thereby, the modulation of surveillance and control strategies for bse. in two experiments calves were dosed orally with a range of amounts of a pool of brainstems from bse-affected cattle. infectivity in the pool was determined by end-point titration in mice. recipient cattle were monitored for clinical disease and, from the inc ... | 2007 | 17374783 |
| risk assessment and surveillance for bovine spongiform encephalopathy. | the national risk of bovine spongiform encephalopathy (bse) has not been assessed by many countries, and many countries are conducting little or no bse surveillance. national measures implemented, including import restrictions, surveillance systems, and sanitary controls, should be based on actual bse risk. thus, as a first step, the national bse status must be determined, particularly through assessment of the national risk. the world organisation for animal health (oie) provides recommendation ... | 2006 | 17361761 |
| conversion of the base prion strain into the bse strain: the origin of bse? | atypical neuropathological and molecular phenotypes of bovine spongiform encephalopathy (bse) have recently been identified in different countries. one of these phenotypes, named bovine "amyloidotic" spongiform encephalopathy (base), differs from classical bse for the occurrence of a distinct type of the disease-associated prion protein (prp), termed prp(sc), and the presence of prp amyloid plaques. here, we show that the agents responsible for bse and base possess different biological propertie ... | 2007 | 17352534 |
| identification of a proteinase k resistant protein for use as an internal positive control marker in prp western blotting. | the routine use of an internal positive control (ipc) marker could prove useful in the diagnosis of transmissible spongiform encephalopathy (tse) diseases, particularly in surveillance programmes where large numbers of negative results are reported. detection of an endogenous ipc protein in a negative sample adds confidence to the correct sample processing throughout the analytical procedure and could avoid the reporting of false negative diagnoses. proteinase k (pk) resistance is one of the key ... | 2007 | 17336356 |
| spongiform encephalopathy in a miniature zebu. | the first case of spongiform encephalopathy in a zebu (bos indicus) was identified in a zoo in switzerland. although histopathologic and immunohistochemical analyses of the central nervous system indicated a diagnosis of bovine spongiform encephalopathy (bse), molecular typing showed some features different from those of bse in cattle (b. taurus). | 2006 | 17326950 |
| on the question of sporadic or atypical bovine spongiform encephalopathy and creutzfeldt-jakob disease. | strategies to investigate the possible existence of sporadic bovine spongiform encephalopathy (bse) require systematic testing programs to identify cases in countries considered to have little or no risk of orally acquired disease or to detect a stable occurrence of atypical cases in countries in which orally acquired disease is disappearing. to achieve 95% statistical confidence that the prevalence for sporadic bse is no greater than 1 per million (i.e., the annual incidence of sporadic creutzf ... | 2006 | 17326930 |
| bovine spongiform encephalopathy and the safety of milk from canadian dairy cattle. | the detection of bovine spongiform encephalopathy (bse) in beef cattle closed canadian beef export markets to 30 countries, including the usa, with devastating financial losses. the detection and confirmation of the fifth and seventh bse-infected animals but first infected dairy cows extended the problem of risk management to canadian dairy farmers. as the public are concerned about the safety not only of beef but also of milk and milk products that may contain disease-causing prions, this revie ... | 2007 | 17308017 |
| functional relevance of dna polymorphisms within the promoter region of the prion protein gene and their association to bse infection. | transmissible spongiform encephalopathies (tses) are a group of neurodegenerative diseases that can occur spontaneously or can be caused by infection or mutations within the prion protein gene prnp. nonsynonymous dna polymorphisms within the prnp gene have been shown to influence susceptibility/resistance to infection in sheep and humans. analysis of dna polymorphisms within the core promoter region of the prnp gene in four major german bovine breeds resulted in the identification of both snps a ... | 2007 | 17255470 |
| multivariate feature selection and hierarchical classification for infrared spectroscopy: serum-based detection of bovine spongiform encephalopathy. | a hierarchical scheme has been developed for detection of bovine spongiform encephalopathy (bse) in serum on the basis of its infrared spectral signature. in the first stage, binary subsets between samples originating from diseased and non-diseased cattle are defined along known covariates within the data set. random forests are then used to select spectral channels on each subset, on the basis of a multivariate measure of variable importance, the gini importance. the selected features are then ... | 2007 | 17237926 |
| changes in plasma metabolites and muscle glycogen are correlated to bovine spongiform encephalopathy in infected dairy cattle. | during the clinical phase of bovine spongiform encephalopathy (bse), a significant decrease was observed in the ratio of muscle glycogen to plasma l-lactic acid concentrations in bse infected field case and experimentally infected dairy cattle compared with healthy control cattle (p<0.001), this being due to changes in the concentration of both metabolites in the bse infected cattle compared with the control group. furthermore, the concentration of plasma alanine was significantly increased (p<0 ... | 2007 | 17197001 |
| [study on bovine spongiform encephacitis and current status of safety control of medical devices deriving from animals]. | to introduce the basic information about mad cow disease and the current status of safety control of medical devices derived from mammalian animal tissues. | 2006 | 17191585 |
| fucosyl-gm1a, an endoglycoceramidase-resistant ganglioside of porcine brain. | the use of bovine brain has been prohibited in many countries because of the world-wide prevalence of mad cow disease, and thus porcine brain is expected to be a new source for the preparation of gangliosides. here, we report the presence of a ganglioside in porcine brain which is strongly resistant to hydrolysis by endoglycoceramidase, an enzyme capable of cleaving the glycosidic linkage between oligosaccharides and ceramides of various glycosphingolipids. five major gangliosides (designated pb ... | 2007 | 17167042 |
| brief review on the epidemiology of transmissible spongiform encephalopathies (tse). | transmissible spongiform encephalopathies (tse) form a group of human and animal diseases that share common features such as (a) distinct pathological lesions in the central nervous system, (b) transmissibility at least in experimental settings, and (c) a long incubation period. considerable differences exist in the host range of individual tses, their routes of transmission, and factors influencing the host susceptibility (such as genotype). the objective of this review was to briefly describe ... | 2007 | 17126962 |
| milk yield, age at first calving, and the risk of bse: an analysis at the farm level in france. | the epidemic of bovine spongiform encephalopathy (bse) in france, as in the uk, has affected dairy cattle much more than beef cattle. however, the intensification of dairy herd management as a risk factor for bse has not to date been analyzed. for this purpose, two databases were merged: the french milk records database, and the french bse database, which can be considered as being devoid of notification bias since july 2001, when systematic tests were implemented. only pure holstein herds were ... | 2007 | 17097171 |
| a review of genetic resistance to disease in bos taurus cattle. | cattle show considerable variability in their responses to a wide range of disease challenges, and much of the variability is genetic. this review highlights genetic variation in disease susceptibility in bos taurus cattle, with variation found at the breed level and also within breeds. disease challenges come from bacteria and viruses, parasites and feed-borne toxins. for an animal to survive, it needs its own mechanisms for resisting these challenges, or for being resilient to them, or it must ... | 2007 | 17095270 |
| radiological assessment of creutzfeldt-jakob disease. | creutzfeldt-jakob disease is a rare fatal neurodegenerative disorder, characterized by rapidly progressive dementia and neurological signs. there is a need for early and accurate clinical diagnosis in order to exclude any treatable disorder. additionally, it is of public interest to differentiate the sporadic form of the disease from the variant cjd type (vcjd), which is probably transmitted from cattle infected with bovine spongiform encephalopathy (bse). high signal in the striatum on t2-weigh ... | 2007 | 17093966 |
| prion gene haplotypes of u.s. cattle. | bovine spongiform encephalopathy (bse) is a fatal neurological disorder characterized by abnormal deposits of a protease-resistant isoform of the prion protein. characterizing linkage disequilibrium (ld) and haplotype networks within the bovine prion gene (prnp) is important for 1) testing rare or common prnp variation for an association with bse and 2) interpreting any association of prnp alleles with bse susceptibility. the objective of this study was to identify polymorphisms and haplotypes w ... | 2006 | 17092337 |
| isolation from cattle of a prion strain distinct from that causing bovine spongiform encephalopathy. | to date, bovine spongiform encephalopathy (bse) and its human counterpart, variant creutzfeldt-jakob disease, have been associated with a single prion strain. this strain is characterised by a unique and remarkably stable biochemical profile of abnormal protease-resistant prion protein (prp(res)) isolated from brains of affected animals or humans. however, alternate prp(res) signatures in cattle have recently been discovered through large-scale screening. to test whether these also represent sep ... | 2006 | 17054396 |
| bse inoculation to prion diseases-resistant sheep reveals tricky silent carriers. | the possible transmission of bovine spongiform encephalopathy (bse) agent to sheep contributed to select genetically sheep considered as resistant to prion diseases i.e., with prp arr/arr genotype. here, we report the infection of two prp arr/arr genotype sheep using the cattle bse agent inoculated by peripheral routes. disease-associated prion protein (prp(d)) was detected in the brain for one case (at 2191 days post-infection (dpi)) and only in the nervous enteric system for the other one (at ... | 2006 | 17049491 |
| comparison of dna variants in the prnp and nf1 regions between bovine spongiform encephalopathy and control cattle. | dna from 252 bovine spongiform encephalopathy (bse) cattle and 376 non-diseased control cattle were genotyped for nine loci in the prion protein (prnp) gene region, three loci in the neurofibromin 1 (nf1) region and four control loci on different chromosomes. the allele and genotype frequencies of the control loci were similar in bse and control cattle. in the analysed 7.4 mb prnp region, the largest differences between bse and control cattle were found for the loci reg2, r16 and r18, which are ... | 2006 | 16978176 |
| relative levels of semen platelet activating factor-receptor (pafr) and ubiquitin in yearling bulls with high content of semen white blood cells: implications for breeding soundness evaluation. | high content of the platelet activating factor (paf) and its plasma membrane receptor (pafr) in semen is thought to benefit fertility in farm animals and humans. we used flow cytometric, biochemical, and immunocytochemical analysis to examine pafr levels alone (trial 1, n = 156 bulls) or in a dual assay with sperm defect marker ubiquitin (ubi; trial 2, n = 88 bulls), in semen samples from 160 yearling bulls undergoing breeding soundness evaluations (bse). in both trials, we observed increased pa ... | 2007 | 16928895 |
| from rabies to transmissible spongiform encephalopathies: an immune-mediated microbial trigger involving molecular mimicry could be the answer. | the concept of experimental allergic encephalomyelitis (eae) being linked to both rabies post-vaccination encephalomyelitis and multiple sclerosis (ms) has raised the intriguing question whether animal studies carried out for the induction and transmission of transmissible spongiform encephalopathies (tses) using brain antigens including prions do have a similar immunopathogenetic mechanism. although an essential link between autoimmunity and ms has been well established, its role in the pathoge ... | 2007 | 16920276 |
| increased expression of water channel aquaporin 1 and aquaporin 4 in creutzfeldt-jakob disease and in bovine spongiform encephalopathy-infected bovine-prp transgenic mice. | spongiform change is a cardinal feature in transmissible spongiform encephalopathies, including creutzfeldt-jakob disease (cjd) and bovine spongiform encephalopathy (bse). it is characterized by swelling of the neuronal processes and vacuolization of the neuropil, leading to increased intraneuronal water content. the present study examines, by gel electrophoresis and western blotting, the expression levels of the water channels aquaporin 1 (aqp1) and aquaporin 4 (aqp4) in the frontal cortex (are ... | 2006 | 16871401 |
| a country that never had a bse crisis: consensus and tensions in transforming the norwegian food system. | norway is often described as a country where the safety of domestically produced food is not questioned and where there is a prevailing consensus about the division of responsibility for food safety. for this reason it was surprising to find that norwegian consumers trust the safety of their meat less than do their british counterparts. this result is particularly interesting, as norway is one of the few countries that has never experienced bse, while britain has been the country most affected b ... | 2006 | 16828930 |
| variant cjd (vcjd) and bovine spongiform encephalopathy (bse): 10 and 20 years on: part 2. | up until february 2006, variant cjd (vcjd), the human disease associated with transmission of bse from cattle, has been confirmed in 160 patients resident in the uk and 28 elsewhere, some of whom have never visited the uk. cases have been reported in france (16 cases), ireland (3), usa (2), canada, italy, japan, the netherlands, portugal, saudi arabia and spain (1 each). the presumed main period of hazard for ingestion of the bse agent in bovine products in the uk is 1984-89, or perhaps up to 19 ... | 2006 | 16823692 |
| sequential pressurized liquid extraction to determine brain-originating fatty acids in meat products as markers in bovine spongiform encephalopathy risk assessment studies. | a new approach using sequential pressurized liquid extraction described recently [j. poerschmann, r. carlson, j. chromatogr. a, 1127 (2006) 18-25] was applied to determine lipid markers originating from central nervous system (cns) tissue of cows in heat-processed sausages. these studies are very important in quality control as well as risk assessment studies in the face of the bovine spongiform encephalopathy (bse) crisis. diagnostic cns lipid markers, which should not be present in meat produc ... | 2006 | 16782118 |
| prp(d) accumulation in organs of arq/arq sheep experimentally infected with bse by peripheral routes. | to study the pathogenesis of bovine spongiform encephalopathy infection in small ruminants, two lacaune sheep with the aa136rr154qq171 and one with the aa136rr154rr171 genotype for the prion protein, were inoculated with a brain homogenate from a french cattle bse case by peripheral routes. sheep with the arq/arq genotype are considered as susceptible to prion diseases contrary to those with the arr/arr genotype. the accumulation of disease-associated prion protein (prp(d)) was analysed by bioch ... | 2006 | 16770445 |
| monitoring for bovine spongiform encephalopathy in sheep in great britain, 1998-2004. | bovine spongiform encephalopathy (bse) may have been transmitted to british sheep via contaminated feed in the 1980s. strain-typing techniques based on immunohistochemical (ihc) detection of abnormal protein (prp(d)) and the molecular analysis of proteinase-resistant protein (prp(res)) by western blotting (wb) can discriminate between natural or experimental scrapie and experimental bse in sheep. between 1 january 1998 and 31 october 2001, 1247 sheep, clinically suspected of scrapie, were found ... | 2006 | 16760414 |
| breed predisposition for bse: epidemiological evidence in bavarian cattle. | we present epidemiological data from bavaria that indicates that animals of the brown swiss (bs) cattle breed might be more susceptible to bse than animals from other breeds, both in terms of disease prevalence and length of the incubation period. bs animals were disproportionately represented among the bse cases (bs represented about 9% of the susceptible population but 27% of actual cases). bs were slaughtered at a higher age (5.8 years vs. 5.0 years for other breeds), and there is a higher pr ... | 2006 | 16739897 |
| brahman bull fertility in a north australian rangeland herd. | low and variable bull fertility was identified as a constraint on reproductive rates in beef cattle grazed in an extensive, multiple sire mating regimen on mount bundey station in the darwin pastoral district of northwestern australia. erratic conception patterns were attributed to a high proportion of bulls with low breeding soundness evaluation scores (bse), a high proportion of aged bulls (40%>8 yr), and to running bulls of mixed age groups. liveweight, scrotal circumference (sc) and age were ... | 1989 | 16726675 |
| a quantitative assessment of the risk of exposure to bovine spongiform encephalopathy via meat-and-bone meal in japan. | the feeding of meat-and-bone meal (mbm) derived from cattle infected with bovine spongiform encephalopathy (bse) is a major source of bse infection. the risks of bse infection via mbm in japan were examined quantitatively to estimate infectivity to cattle via mbm derived from a single clinically infected animal being rendered. three routes of exposure were modeled: (i) feeding cattle concentrates containing mbm as an ingredient, (ii) feeding cattle concentrates contaminated with mbm from non-rum ... | 2006 | 16712985 |
| gene expression alterations in brains of mice infected with three strains of scrapie. | transmissible spongiform encephalopathies (tses) or prion diseases are fatal neurodegenerative disorders which occur in humans and various animal species. examples include creutzfeldt-jakob disease (cjd) in humans, bovine spongiform encephalopathy (bse) in cattle, chronic wasting disease (cwd) in deer and elk, and scrapie in sheep, and experimental mice. to gain insights into tse pathogenesis, we made and used cdna microarrays to identify disease-associated alterations in gene expression. brain ... | 2006 | 16700923 |
| an evaluation of united kingdom environmental bovine spongiform encephalopathy risk assessment. | as a member of the group of diseases known as transmissible spongiform encephalopathies (tses), bovine spongiform encephalopathy (bse) has been causally associated with a new variant of creutzfeldt-jakob disease (vcjd) in humans. given the many uncertainties on the transmission and persistence of tse pathogens in the environment, quantitative assessment of risks to humans and animals continues to remain a public health issue. this paper reviews quantitative bse risk assessments undertaken in the ... | 2005 | 16639897 |
| distribution of prp(sc) in cattle with bovine spongiform encephalopathy slaughtered at abattoirs in japan. | three 80- to 95-month-old holstein dairy cattle infected naturally with the agent of bovine spongiform encephalopathy (bse) and slaughtered at abattoirs in japan were examined for the distribution of disease-specific and protease-resistant prion protein (prp(sc)) by immunohistochemistry (ihc) and western blot (wb) analyses. the cattle showed no clinical signs or symptoms relevant to bse but were screened as positive by enzyme-linked immunosorbent assay, a rapid test for bse. this positive result ... | 2006 | 16632909 |
| bse infection in bovine prp transgenic mice leads to hyperphosphorylation of tau-protein. | we observed the changes in the central nervous system (cns) of transgenic mice expressing bovine prion protein (bo-prp) as a contribution to our knowledge of the pathogenesis of bovine spongiform encephalopathy (bse). the main result was the detection of hyperphosphorylated tau. this protein was detected for the first time, using immunohistochemical techniques, in the neurons and glial cells of mice experimentally infected with bse. the results highlighted the involvement of tau protein in the p ... | 2006 | 16621340 |
| safety evaluation for a biodiesel process using prion-contaminated animal fat as a source. | due to the bovine spongiform encephalopathy (bse), specified risk material (srm) as well as animal meat and bone meal (mbm) are banned from the food and feed chain because of a possible infection with pathogenic prions (prp(sc)). nowadays, prions are widely accepted to be responsible for tse(transmissible spongiform encephalopathies)-caused illnesses like bse and scrapie, and especially for the occurrence of the new variant of cjd in humans. presently, srm and mbm are burnt under high temperatur ... | 2006 | 16612902 |
| development of recombinant chicken igy from single chain fragment of variable region for diagnosis of bse. | we generated two recombinant chicken igys, designated ab3-15 and ab4-19, against mammalian prion protein (prp) from the single chain fragment of variable region (scfv) antibodies. these two antibodies recognized prp(sc) from bovine spongiform encephalopathy (bse) in cattle and were more sensitive than the corresponding scfv antibodies. these antibodies also recognized prp(sc) from other scrapie-infected mammals. these results indicate that ab3-15 and ab4-19 are useful for diagnosis of bse as wel ... | 2007 | 16580230 |
| inactivation of transmissible spongiform encephalopathy agents in food products by ultra high pressure-temperature treatment. | bovine spongiform encephalopathy (bse) contamination of the human food chain most likely resulted from nervous system tissue in mechanically recovered meat used in the manufacture of processed meats. the availability of effective decontamination methods for products considered at risk for bse or other transmissible spongiform encephalopathies (tses) would be an attractive safeguard to human health, but neither of the two proven inactivating methods, autoclaving or exposure to strong alkali or bl ... | 2006 | 16542885 |
| sequence variation of bovine prion protein gene in japanese cattle (holstein and japanese black). | to assess relationships between nucleotide polymorphisms of the prion protein (prnp) gene and susceptibility to bovine spongiform encephalopathy (bse), we investigated polymorphisms in the open reading frame (orf) and 2 upper regions of the prnp gene from 2 japanese cattle breeds: 863 healthy holstein cattle, 6 bse-affected holstein cattle, and 186 healthy japanese black (jb) cattle. in the orf, we found single-nucleotide polymorphisms (snps) at nucleotide positions 234 and 576 and found 5 or 6 ... | 2006 | 16462113 |
| evaluation of the cumulative evidence for freedom from bse in birth cohorts. | substantial resources are used for surveillance of bovine spongiform encephalopathy (bse) despite an extremely low detection rate, especially in healthy slaughtered cattle. we have developed a method based on the geometric waiting time distribution to establish and update the statistical evidence for bse-freedom for defined birth cohorts using continued surveillance data. the results suggest that currently (data included till september 2004) a birth cohort of danish cattle born after march 1999 ... | 2006 | 16450206 |
| managing the risk of transmission of variant creutzfeldt jakob disease by blood products. | whereas plasma-derived clotting factor concentrates now have a very good safety record for not being infectious for lipid enveloped viruses, concern has arisen about the possibility that prion diseases might be transmitted by blood products. there is epidemiological evidence that classical sporadic creutzfeld jakob disease (cjd) is not transmitted by blood transfusion. there is now good evidence that the abnormal prion associated with variant cjd can be transmitted by transfusion of fresh blood ... | 2006 | 16371015 |
| bovine spongiform encephalopathy statement of possible relation with new variant creutzfeldt-jakob disease: effects on the welfare of united kingdom cattle. | although measures to control bovine spongiform encephalopathy (bse) had been in force in the united kingdom for many years and had resulted in a marked decline in clinical cases, the announcement by the secretary of state for health on march 20, 1996, that a new variant form of creutzfeldt-jakob disease may be linked with exposure to bse, resulted in the introduction of several new control measures. these measures included a scheme banning human consumption of meat from cattle who were more than ... | 1998 | 16363943 |
| ovine infection with the agents of scrapie (ch1641 isolate) and bovine spongiform encephalopathy: immunochemical similarities can be resolved by immunohistochemistry. | immunochemical ("rapid") tests, which recognize a partly protease-resistant conformer of the prion protein (prp(res)) are now widely used in europe for the diagnosis of transmissible spongiform encephalopathies (tses). some of these tests can be used to distinguish natural scrapie from experimental bovine spongiform encephalopathy (bse) in sheep, on the basis of migration pattern differences of prp(res) in western immunoblots. however, prp(res) from sheep inoculated with ch1641 scrapie gives an ... | 2006 | 16324707 |
| meningoencephalitis tuberculosa in a holstein cow. | the gross and histopathologic lesions of meningoencephalitis tuberculosa in a 4-year-old holstein cow showing clinical signs compatible with bovine spongiform encephalopathy are described in this report. grossly, numerous gray to yellow, firm and caseous nodules were seen on the ventral surfaces of the brain and in the lateral and fourth ventricles. histopathologically, foci of caseation and dystrophic mineralization were surrounded by multinucleated giant cells, epitheloid macrophages, plasma c ... | 2005 | 16301587 |
| dna polymorphisms of the prion doppel gene region in four different german cattle breeds and cows tested positive for bovine spongiform encephalopathy. | polymorphisms of the prion protein gene prnp have been shown to influence the susceptibility/resistance to prion infections in human and sheep. in addition, the t174m polymorphism within the flanking prion doppel gene (prnd) was thought to be involved in susceptibility to sporadic creutzfeldt-jacob disease. to study a possible influence of dna polymorphisms of the bovine prnd gene in bovine spongiform encephalopathy (bse), previously identified and newly isolated dna polymorphisms were genotyped ... | 2005 | 16284804 |
| polymorphisms of the prion protein gene (prnp) in hanwoo (bos taurus coreanae) and holstein cattle. | polymorphisms in the prion protein gene (prnp) in humans and sheep correlate with susceptibility to transmissible spongiform encephalopathies (tses). bovine spongiform encephalopathy (bse) has been reported in british and japanese cattle; it has occurred thus far in holstein cattle. bse in hanwoo (bos taurus coreanae) cattle has not been diagnosed up to now. to characterize the bovine prnp polymorphisms in korean cattle, we analyzed the open reading frame (orf) of prnp in 120 hanwoo (beef) cattl ... | 2005 | 16284424 |
| risk perception and communication: lessons for the food and food packaging industry. | health risks are front-page news. be it bovine spongiform encephalitis (bse), surface ozone, or radiation from transmitter stations or mobile phones, the popular press puts out a constant stream of risk warnings and sensational reports about potential health threats. this paper examines how the general public perceives and assesses such information when it comes to food and food packaging risks. in the first part, the basic components of food risks are discussed and then compared with the percep ... | 2005 | 16227190 |
| detection of metazoan species as a public health issue: simple methods for the validation of food safety and quality. | species identification represents a critical issue in food chain safety and quality control. several procedures are available to detect animal proteins in cattle feed or to trace transgenic foods. the most effective approach is based on the use of dna as a marker. amplification of dna provides rapid, sensitive and specific protocols. several target genes can be used, but new insights come from the mitochondrial genome, which is naturally amplified in each cell and shows a remarkable resistance t ... | 2005 | 16216782 |
| characterization and enzymatic degradation of sup35nm, a yeast prion-like protein. | transmissible spongiform encephalopathies (tses) are believed to be caused by an unconventional infectious agent, the prion protein. the pathogenic and infectious form of prion protein, prpsc, is able to aggregate and form amyloid fibrils, very stable and resistant to most disinfecting processes and common proteases. under specific conditions, prpsc in bovine spongiform encephalopathy (bse) brain tissue was found degradable by a bacterial keratinase and some other proteases. since this disease-c ... | 2005 | 16081647 |
| bov-ta short interspersed nucleotide element sequences in circulating nucleic acids from sera of cattle with bovine spongiform encephalopathy (bse) and sera of cattle exposed to bse. | circulating nucleic acids (cna) are known to be enriched in repetitive dna sequences in humans. here, bovine sera cna were analyzed to determine if cell stress-related short interspersed nucleotide elements (sines) could be detected in sera from cattle associated with bovine spongiform encephalopathy (bse). nucleic acids were extracted, amplified, cloned, and sequenced from the sera of protease-resistant prion protein (prp(res))-positive cattle (n = 2) and sera from bse-cohort cows (n = 6); 150 ... | 2005 | 16002628 |
| frequency of brain tissue embolism associated with captive bolt gun stunning of sheep. | in accordance with controls instituted to protect the consumer from meat potentially infected with bovine spongiform encephalopathy (bse), brain tissue emboli caused by the use of captive bolt gun (cbg) stunning have been identified as a potential public health risk that requires further investigation. as the natural occurrence of bse in sheep remains uncertain we have investigated the frequency of brain tissue embolism associated with stunning by two types of cbg that are in commercial use in t ... | 2004 | 15992291 |
| fertility of yearling beef bulls during mating. | crossbred (bos taurus) yearling beef bulls were assessed for breeding soundness and physical traits prior to multi-sire natural mating at pasture. bulls (n = 60) were assigned to six groups of nine or 10 bulls and two bull-groups were rotated on 14-day intervals during a 63-day mating season in each breeding herd (n = 3) of 191-196 cows. the remaining bulls (n = 14) were maintained under similar environmental conditions without mating exposure. bulls were observed during mating and assessed for ... | 2005 | 15961148 |
| differentiation of bovine from porcine gelatines using polyclonal anti-peptide antibodies in indirect and competitive indirect elisa. | gelatine is a collagen derivative obtained from bones and hides/skin mainly from bovine and pigs. as a consequence of the outbreak of bovine spongiform encephalopathy (bse), the use of bovine gelatine in feed, food and pharmaceutical products has been restricted by regulatory authorities. however, no method was presently available for its specific detection. the large similarity in amino-acid sequences of collagens from different species make their immunochemical differentiation difficult when u ... | 2005 | 15899572 |
| expression of prion protein in the gut of mice infected orally with the 301v murine strain of the bovine spongiform encephalopathy agent. | transmissible spongiform encephalopathies (tses) are characterized by the accumulation of an abnormal, disease-associated prion protein (prp(d)). expression of its normal cellular counterpart (prp(c)) by the host is a pre-requisite for the spread of infection to the central nervous system and the development of disease. moreover, cells expressing prp(c) at specific sites such as the gastrointestinal tract might be regarded as the initial point of prp(c)-prp(d) conversion after infection by the o ... | 2005 | 15893985 |
| [variant creutzfeldt-jakob disease in france: estimating the number of cases related to travel to the united kingdom between 1980 and 1995]. | the outbreak of variant creutzfeldt-jakob disease (vcjd) cases rose serious concerns about secondary transmission of the disease, particularly through blood transfusion. protective measures leading to the exclusion of potentially infectious blood donors were settled: in france, donors who had stayed more than one year in the uk were excluded. in this work, which was part of a larger study aiming to estimate the french epidemic of vcjd, the number of vcjd cases who were infected during a trip to ... | 2005 | 15888987 |
| [the requirements of society in terms of the safety of food of animal origin--a reflection on the case of france]. | are consumers obsessed by 'zero risk'? is their relationship to food of animal origin irrational? the comments and reactions during the bovine spongiform encephalopathy (bse) crisis that began in november 2000 certainly suggest so. however, sociological surveys conducted at the height of the crisis refute this impression: judging by individual perceptions, risk is not an obsession; it is only one of several aspects of people's relationship to animal products. furthermore, concern about healthy e ... | 2003 | 15884575 |
| transmission barriers for bovine, ovine, and human prions in transgenic mice. | transgenic (tg) mice expressing full-length bovine prion protein (boprp) serially propagate bovine spongiform encephalopathy (bse) prions without posing a transmission barrier. these mice also posed no transmission barrier for suffolk sheep scrapie prions, suggesting that cattle may be highly susceptible to some sheep scrapie strains. tg(boprp) mice were also found to be susceptible to prions from humans with variant creutzfeldt-jakob disease (cjd); on second passage in tg(boprp) mice, the incub ... | 2005 | 15827140 |
| testing the possibility to protect bovine prpc transgenic swiss mice against bovine prpsc infection by dna vaccination using recombinant plasmid vectors harboring and expressing the complete or partial cdna sequences of bovine prpc. | the objective of this study was to investigate the molecular mechanisms of neurobiological processes involved in the degeneration of the central nervous system. the bovine spongiform encephalopathy (bse) was used as experimental model system for investigation of transmissible spongiform encephalopathy (tse). the experimental strategy was to evaluate the possibility for protection of bovine prp(c) transgenic mice against a bovine prp(sc) infection by dna vaccination using the complete or partial ... | 2005 | 15744583 |
| phenotype of disease-associated prp accumulation in the brain of bovine spongiform encephalopathy experimentally infected sheep. | in view of the established link between bovine spongiform encephalopathy (bse) and variant creutzfeldt-jakob disease and of the susceptibility of sheep to experimental bse, the detection of potential cases of naturally occurring bse in sheep has become of great importance. in this study, the immunohistochemical (ihc) phenotype of disease-associated prion protein (prp(d)) accumulation has been determined in the brain of 64 sheep, of various breeds and prp genotypes, that had developed neurologica ... | 2005 | 15722546 |
| thinking the unthinkable: alzheimer's, creutzfeldt-jakob and mad cow disease: the age-related reemergence of virulent, foodborne, bovine tuberculosis or losing your mind for the sake of a shake or burger. | the possibility of the age-related reemergence of foodborne mycobacterium bovis (bovine tuberculosis) as a vector for creutzfeldt-jakob disease (cjd or human mad cow disease) and mad cow disease itself is real. the cdc reported last may of an outbreak of cjd linked to the consumption of meat contaminated "with the agent causing" bovine spongiform encephalopathy (bse) in a new jersey racetrack between the time frame 1995-2004. in the opinion of experts, ample justification exists for considering ... | 2005 | 15694685 |
| estimation of the age-dependent risk of infection to bse of dairy cattle in great britain. | an important aspect of the bovine spongiform encephalopathy (bse) epidemic has been an apparent age-dependent risk of infection, with younger cattle being more likely to become infected than older cattle. our objective was to determine the age-dependent risk of infection of dairy cattle. we first reviewed unpublished data on the feeding patterns of proprietary concentrates for dairy-replacement cattle. these data showed that autumn- and spring-born cattle would receive different feeding patterns ... | 2004 | 15579333 |
| distribution of prp(res) in the brains of bse-affected cows detected by active surveillance in catalonia, spain. | 2004 | 15551927 | |
| prevalence of bse in cattle found dead, euthanased or emergency slaughtered on farms in western france in 2000, 2001 and 2002. | the overall trend and the trend within birth cohorts of the prevalence of bovine spongiform encephalopathy (bse) in cattle found dead, euthanased or emergency slaughtered on farms in the bretagne, basse normandie and pays de la loire regions of france, during the periods from august 7 to december 22 in 2000, 2001 and 2002, were analysed by non-conditional logistic regression, adjusted for the region and for the type of animals. the overall prevalence of bse during these three periods decreased f ... | 2004 | 15537142 |
| the first canadian indigenous case of bovine spongiform encephalopathy (bse) has molecular characteristics for prion protein that are similar to those of bse in the united kingdom but differ from those of chronic wasting disease in captive elk and deer. | brain tissue from a case of bovine spongiform encephalopathy (bse) from alberta was subjected to a western immunoblotting technique to ascertain the molecular profile of any disease-specific, abnormal prion protein, that is, prion protein that is protease-resistant (prp(res)). this technique can discriminate between isolates from bse, ovine scrapie, and sheep experimentally infected with bse. isolates of brain tissue from the bse case in alberta, 3 farmed elk with chronic wasting disease (cwd) f ... | 2004 | 15532881 |
| comparative prnp genotyping of u.s. cattle sires for potential association with bse. | the recent discovery of significant associations between bovine spongiform encephalopathy (bse) susceptibility in german cattle and the frequency distributions of insertion/deletion (indel) polymorphisms within the bovine prnp gene prompted an evaluation of 132 commercial u.s. artificial insemination (ai) sires from 39 breeds. forward primer sequences from published primer sets targeting indels within the putative bovine prnp promoter, intron 1, and the 3' utr (untranslated region) were synthesi ... | 2004 | 15520885 |
| prion protein gene (prnp) variants and evidence for strong purifying selection in functionally important regions of bovine exon 3. | amino acid replacements encoded by the prion protein gene (prnp) have been associated with transmissible and hereditary spongiform encephalopathies in mammalian species. however, an association between bovine spongiform encephalopathy (bse) and bovine prnp exon 3 has not been detected. moreover, little is currently known regarding the mechanisms of evolution influencing the bovine prnp gene. therefore, in this study we evaluated the patterns of nucleotide variation associated with prnp exon 3 fo ... | 2004 | 15477588 |
| the significance of using pooled human serum in human articular cartilage tissue engineering. | animal serum is commonly used in chondrocytes culture expansion to promote cell proliferation and shorten the time lag before new tissue reconstruction is possible. however, animal serum is not suitable for regeneration of clinical tissue because it has potential risk of viral and prion related disease transmission particularly mad cow disease and foreign protein contamination that can stimulate immune reaction leading to graft rejection. in this context, human serum as homologous supplement has ... | 2004 | 15468795 |
| analysis of 2000 consecutive uk tonsillectomy specimens for disease-related prion protein. | variant creutzfeldt-jakob disease (cjd) is thought to be caused by dietary or other exposure to bovine spongiform encephalopathy (bse) prions. the prevalence of preclinical or subclinical prion infection in the uk is currently unknown. since clinical variant cjd is uniformly associated with tonsillar prion infection, we screened 2000 anonymous surgical tonsillectomy specimens for disease-associated prion protein. analysis by both high sensitivity immunoblotting and immunohistochemistry detected ... | 2004 | 15464187 |
| classification of signatures of bovine spongiform encephalopathy in serum using infrared spectroscopy. | signatures of bovine spongiform encephalopathy (bse) have been identified in serum by means of "diagnostic pattern recognition (dpr)". for dpr-analysis, mid-infrared spectroscopy of dried films of 641 serum samples was performed using disposable silicon sample carriers and a semi-automated dpr research system operating at room temperature. the combination of four mathematical classification approaches (principal component analysis plus linear discriminant analysis, robust linear discriminant ana ... | 2004 | 15457319 |
| genetic studies in relation to kuru: an overview. | kuru is a subacute neurodegenerative disease presenting with limb ataxia, dysarthria, and a shivering tremor. the disease progress to complete motor and mental incapacity and death within 6 to 24 months. neuropathologically, a typical pattern of neuronal loss, astrocytic and microglial proliferation, characteristic "kuru-type" amyloid plaques, and prp deposits in the cerebral cortex and cerebellum are observed. kuru is the prototype of a group of human transmissible spongiform encephalopathies ( ... | 2004 | 15354868 |
| the role of prp in health and disease. | transmissible spongiform encephalopathies (tses) such as scrapie in sheep, bovine spongiform encephalopathy (bse) in cattle or creutzfeldt-jacob disease (cjd) and gerstmann-sträussler-scheinker syndrome (gss) in humans, are caused by an infectious agent designated prion. the "protein only" hypothesis states that the prion consists partly or entirely of a conformational isoform of the normal host protein prpc and that the abnormal conformer, when introduced into the organism, causes the conversio ... | 2004 | 15354865 |
| is mad cow disease caused by a bacteria? | transmissible spongioform enchephalopathies (tse's), include bovine spongiform encephalopathy (also called bse or "mad cow disease"), creutzfeldt-jakob disease (cjd) in humans, and scrapie in sheep. they remain a mystery, their cause hotly debated. but between 1994 and 1996, 12 people in england came down with cjd, the human form of mad cow, and all had eaten beef from suspect cows. current mad cow diagnosis lies solely in the detection of late appearing "prions", an acronym for hypothesized, ge ... | 2004 | 15325025 |
| impaired motor coordination on static rods in bse-infected mice. | scrapie and bovine spongiform encephalopathy (bse) are both progressive neurodegenerative diseases that are transmissible to mice. the onset of clinical symptoms is more subtle and variable in murine bse than in murine scrapie. assessment of behavioural changes that occur throughout disease would aid early diagnosis of disease so that more consistent end points could be made and potential therapies could be investigated. c57bl/6j mice inoculated via the intraperitoneal route with 301c bse or con ... | 2004 | 15302135 |
| bovine spongiform encephalopathy and creutzfeldt-jakob disease: facts and uncertainties underlying the causal link between animal and human diseases. | following an outbreak of bovine spongiform encephalopathy (bse) in dairy cows in the united kingdom (uk), 153 definite and probable human cases of new variant creutzfeldt-jakob disease (nvcjd) have been reported, almost exclusively in the uk. although exposure to the bse agent is the most plausible interpretation for the occurrence of nvcjd, the causal link between the bse prion and nvcjd is still debated. this review discusses the pros and cons of nvcjd as a separate nosographic entity, the sci ... | 2004 | 15300459 |
| identification of a new prion strain in cattle--is there more than one form of bse? | 2004 | 15181930 | |
| do bovine lymphocytes express a peculiar prion protein? | the cellular prion protein (prpc) is a glycolipid-anchored cell surface protein that usually exhibits three glycosylation states. its post-translationally modified isoform, prpsc, is involved in the pathogenesis of various transmissible spongiform encephalopathies (tses). in bovine species, bse infectivity appears to be restricted to the central nervous system; few or no detectable infectivity is found in lymphoid tissues in contrast to scrapie or variant cjd. since expression of prpc is a prere ... | 2002 | 15144021 |
| evaluation of rapid tests for the diagnosis of transmissible spongiform encephalopathies in sheep and goats. | in accordance with eu regulation 999/2001, rapid tests already adopted for bovine spongiform encephalopathy (bse; prionics check western, platelia-bse and enfer tse) are to be applied in all european countries to a sub-population of over 18-month-old slaughtered or dead sheep and goats to improve scrapie surveillance and to determine the possible presence of bse in sheep; however, the three tests have thus far been evaluated only for bse and no official data are available about their performance ... | 2004 | 15067554 |
| trust in food in the age of mad cow disease: a comparative study of consumers' evaluation of food safety in belgium, britain and norway. | how do food scandals like dioxines in food in belgium (summer 1999) and the detection of mad cow disease (bse) in britain affect consumers' confidence in food safety? in this paper, based on three thousand telephone interviews during the last quarter of 1999, consumers in belgium and britain are compared with consumers in norway, where there has been no such serious food scandal in recent years. 'trust' is a diffuse and complex concept to measure. in this article a consumer trust typology is dev ... | 2004 | 15036780 |
| comparative molecular analysis of the abnormal prion protein in field scrapie cases and experimental bovine spongiform encephalopathy in sheep by use of western blotting and immunohistochemical methods. | since the appearance of bovine spongiform encephalopathy (bse) in cattle and its linkage with the human variant of creutzfeldt-jakob disease, the possible spread of this agent to sheep flocks has been of concern as a potential new source of contamination. molecular analysis of the protease cleavage of the abnormal prion protein (prp), by western blotting (prp(res)) or by immunohistochemical methods (prp(d)), has shown some potential to distinguish bse and scrapie in sheep. using a newly develope ... | 2004 | 15016886 |