Publications

effect of prp genotype and route of inoculation on the ability of discriminatory western blot to distinguish scrapie from sheep bovine spongiform encephalopathy.	procedures for discriminating scrapie from bovine spongiform encephalopathy (bse) in sheep are relevant to ascertain whether bse has entered the sheep population. this study was aimed at investigating whether the suitability of an official eu discriminative method is affected by the sheep prp genotype and route of infection.	2011	21994325
BSE: where are we now?		2011	21965237
experimental interspecies transmission studies of the transmissible spongiform encephalopathies to cattle: comparison to bovine spongiform encephalopathy in cattle.	prion diseases or transmissible spongiform encephalopathies (tses) of animals include scrapie of sheep and goats; transmissible mink encephalopathy (tme); chronic wasting disease (cwd) of deer, elk and moose; and bovine spongiform encephalopathy (bse) of cattle. the emergence of bse and its spread to human beings in the form of variant creutzfeldt-jakob disease (vcjd) resulted in interest in susceptibility of cattle to cwd, tme and scrapie. experimental cross-species transmission of tse agents p ...	2011	21908269
White-tailed deer are susceptible to the agent of sheep scrapie by intracerebral inoculation.	ABSTRACT: Interspecies transmission studies afford the opportunity to better understand the potential host range and origins of prion diseases. The purpose of this experiment was to determine susceptibility of white-tailed deer to the agent of scrapie after intracerebral inoculation and to compare clinical signs and lesions to those reported for chronic wasting disease (CWD). Deer (n = 5) were inoculated with 1 mL of a 10% (wt/vol) brain homogenate derived from a sheep clinically affected with s ...	2011	21988781
heart rate variability analysis in sheep affected by transmissible spongiform encephalopathies.	abstract:	2011	22168827
prpsc detection in formalin-fixed paraffin-embedded tissue by elisa.	abstract:	2011	22018205
immunophenotype of cells within cervine rectoanal mucosa-associated lymphoid tissue and mesenteric lymph nodes.	rectoanal mucosa-associated lymphoid tissue (ramalt) is a part of the lymphoid system that can be sampled easily in live animals, especially ruminants. ramalt biopsy is useful for the diagnosis of transmissible spongiform encephalopathies, including scrapie in sheep and goats and chronic wasting disease (cwd) in cervids. diagnosis is reliant on detection of abnormal prion protein (prp(d)), which is associated with lymphoid follicles. for enzyme linked immunosorbent assays (elisas) detecting prp( ...	2011	22000034
changes induced by natural scrapie in the calretinin-immunopositive cells and fibres of the sheep cerebellar cortex.	calretinin (cr)-immunopositive cells and fibres in the cerebellar cortex (vermal archicerebellum and neocerebellum) of scrapie-affected, arq/arq, rasa aragonesa breed sheep were studied in comparison with healthy, young and aged, arq/arq, rasa aragonesa animals and with manchega breed sheep. the scrapie-affected sheep showed signs of both cellular involution and hypertrophic/hyperimmunoreactive responses in all neuronal subtypes; the distribution of the neuronal subtypes in the archi- and neocer ...	2011	22116659
prionemia and leuco-platelet associated infectivity in sheep tse models.	the dynamics of the circulation and distribution of transmissible spongiform encephalopathy (tse) agents in the blood of infected individuals remain largely unknown. this clearly limits the understanding of role of blood in tse pathogenesis and the development of reliable tse blood detection assay.using two distinct sheep scrapie models and blood transfusion, this work demonstrates the occurrence of a very early and persistent prionemia. this ability to transmit disease by blood transfusion was ...	2011	22156536
Mechanisms of prion disease progression: a chemical reaction network approach.	Fatal neurodegenerative diseases such as bovine spongiform encephalopathy in cattle, scrapie in sheep and Creutzfeldt-Jakob disease in humans are caused by prions. Prion is a protein encoded by a normal cellular gene. The cellular form of the prion, namely PrP(C), is benign but can be converted into a disease-causing form (named scrapie), PrP(Sc), by a conformational change from -helix to -sheets. Prions replicate by this conformational change; that is, PrP(Sc) interacts with PrP(C) producing a ...	2011	22129030
Methodology Adaptation of a low-cost medium-throughput genotyping system for ovine prion protein gene polymorphims associated with scrapie.	Resistance and susceptibility to scrapie in sheep have been associated with SNPs located at codons 136, 154 and 171 of the prion protein (PRNP) gene. Many countries have sheep breeding programs selecting for resistance to scrapie based on the genotyping of these SNPs. We adapted a fast and robust method for genotyping sheep flocks for these polymorphisms, with reduced costs. Ninety-six samples were genotyped using an adapted SNaPshot PRNP assay, and the results were checked by resequencin ...	2011	22194174
in vitro prion protein conversion suggests risk of bighorn sheep (ovis canadensis) to transmissible spongiform encephalopathies.	transmissible spongiform encephalopathies (tses) affect both domestic sheep (scrapie) and captive and free-ranging cervids (chronic wasting disease; cwd). the geographical range of bighorn sheep (ovis canadensis; bhs) overlaps with states or provinces that have contained scrapie-positive sheep or goats and areas with present epizootics of cwd in cervids. no tses have been documented in bhs, but the susceptibility of this species to tses remains unknown.	2013	23938169