| Molecular cloning and polymorphism analysis of the prion protein gene in Tan sheep of Ningxia, China. | The resistance or susceptibility of sheep to scrapie is associated with polymorphisms of the prion protein gene (PRNP), particularly, single nucleotide polymorphisms (SNPs) in amino acid positions 136, 154 and 171. The prion protein (PrP) gene sequence and the deduced amino acid alignment of prion protein in Tan sheep, a local Chinese sheep breed traditionally raised in Ningxia, northwestern China, were determined and variability of the PrP amino acids sequence was analyzed in this study. The Pr ... | 2011 | 21722718 |
| PRNP haplotype and genotype frequencies in Brazilian sheep: Issues for conservation and breeding programs. | Polymorphisms of PRNP gene have been strongly correlated to the susceptibility/resistance to scrapie in sheep. Variants at the coding positions 136, 154 and 171 have been the most frequently associated to susceptibility to classical scrapie. The aim of this study was to estimate PRNP haplotype and genotype frequencies in a sample of 1400 sheep from 13 different breeds that are representative of the main production regions in Brazil. A total of four different alleles (ARR, ARQ, AHQ and VRQ) and n ... | 2011 | 21816449 |
| White-tailed deer are susceptible to the agent of sheep scrapie by intracerebral inoculation. | ABSTRACT: Interspecies transmission studies afford the opportunity to better understand the potential host range and origins of prion diseases. The purpose of this experiment was to determine susceptibility of white-tailed deer to the agent of scrapie after intracerebral inoculation and to compare clinical signs and lesions to those reported for chronic wasting disease (CWD). Deer (n = 5) were inoculated with 1 mL of a 10% (wt/vol) brain homogenate derived from a sheep clinically affected with s ... | 2011 | 21988781 |
| prpsc detection in formalin-fixed paraffin-embedded tissue by elisa. | abstract: | 2011 | 22018205 |
| immunophenotype of cells within cervine rectoanal mucosa-associated lymphoid tissue and mesenteric lymph nodes. | rectoanal mucosa-associated lymphoid tissue (ramalt) is a part of the lymphoid system that can be sampled easily in live animals, especially ruminants. ramalt biopsy is useful for the diagnosis of transmissible spongiform encephalopathies, including scrapie in sheep and goats and chronic wasting disease (cwd) in cervids. diagnosis is reliant on detection of abnormal prion protein (prp(d)), which is associated with lymphoid follicles. for enzyme linked immunosorbent assays (elisas) detecting prp( ... | 2011 | 22000034 |
| changes induced by natural scrapie in the calretinin-immunopositive cells and fibres of the sheep cerebellar cortex. | calretinin (cr)-immunopositive cells and fibres in the cerebellar cortex (vermal archicerebellum and neocerebellum) of scrapie-affected, arq/arq, rasa aragonesa breed sheep were studied in comparison with healthy, young and aged, arq/arq, rasa aragonesa animals and with manchega breed sheep. the scrapie-affected sheep showed signs of both cellular involution and hypertrophic/hyperimmunoreactive responses in all neuronal subtypes; the distribution of the neuronal subtypes in the archi- and neocer ... | 2011 | 22116659 |
| characterization of prnp and sprn coding regions from atypical scrapie cases diagnosed in poland. | scrapie, a fatal transmissible spongiform encephalopathy (tse) occurs in two phenotypes: classical and atypical. many authors point out that the polymorphism of three codons (136, 154, 171) of the prnp (prp gene) is associated with a sheep susceptibility to classical scrapie. until now, only one prnp gene variant coding phenylalanine at codon 141 has been found to be associated with atypical scrapie. another recently identified and interesting candidate gene for scrapie susceptibility in sheep i ... | 2011 | 21674189 |
| Methodology Adaptation of a low-cost medium-throughput genotyping system for ovine prion protein gene polymorphims associated with scrapie. | Resistance and susceptibility to scrapie in sheep have been associated with SNPs located at codons 136, 154 and 171 of the prion protein (PRNP) gene. Many countries have sheep breeding programs selecting for resistance to scrapie based on the genotyping of these SNPs. We adapted a fast and robust method for genotyping sheep flocks for these polymorphisms, with reduced costs. Ninety-six samples were genotyped using an adapted SNaPshot PRNP assay, and the results were checked by resequencin ... | 2011 | 22194174 |
| in vitro prion protein conversion suggests risk of bighorn sheep (ovis canadensis) to transmissible spongiform encephalopathies. | transmissible spongiform encephalopathies (tses) affect both domestic sheep (scrapie) and captive and free-ranging cervids (chronic wasting disease; cwd). the geographical range of bighorn sheep (ovis canadensis; bhs) overlaps with states or provinces that have contained scrapie-positive sheep or goats and areas with present epizootics of cwd in cervids. no tses have been documented in bhs, but the susceptibility of this species to tses remains unknown. | 2013 | 23938169 |
| exosome-producing follicle associated epithelium is not involved in uptake of prpd from the gut of sheep (ovis aries): an ultrastructural study. | in natural or experimental oral scrapie infection of sheep, disease associated prion protein (prp(d)) often first accumulates in peyer's patch (pp) follicles. the route by which infectivity reaches the follicles is unknown, however, intestinal epithelial cells may participate in intestinal antigenic presentation by delivering exosomes as vehicles of luminal antigens. in a previous study using an intestinal loop model, following inoculation of scrapie brain homogenate, inoculum associated prp(d) ... | 2011 | 21789232 |
| myenteric neurons of the ileum that express somatostatin are a target of prion neuroinvasion in an alimentary model of sheep scrapie. | neuroinvasion of the enteric nervous system by prions is an important step in dissemination to the brain, yet very little is known about the basic process of enteric neuroinvasion. using an alimentary model of neonatal disease transmission, neuroinvasion by scrapie prions in the ileum of lambs was detected by immunohistochemical staining for the disease-associated form of the prion protein, prpsc. odds ratios (or) were determined for the frequency of prpsc staining within enteric somata categori ... | 2008 | 18427817 |
| prionemia and leuco-platelet associated infectivity in sheep tse models. | the dynamics of the circulation and distribution of transmissible spongiform encephalopathy (tse) agents in the blood of infected individuals remain largely unknown. this clearly limits the understanding of role of blood in tse pathogenesis and the development of reliable tse blood detection assay.using two distinct sheep scrapie models and blood transfusion, this work demonstrates the occurrence of a very early and persistent prionemia. this ability to transmit disease by blood transfusion was ... | 2011 | 22156536 |