Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| prevalence of antibodies to selected viral pathogens in wild boars (sus scrofa) in croatia in 2005-06 and 2009-10. | we determined prevalence of antibody to selected viral pathogens important for domestic pigs and livestock in 556 wild boar (sus scrofa) sera collected during 2005-06 and 2009-10 in four counties in croatia. these counties account for an important part of the croatian commercial pig production and have a high density of wild boars. samples were tested for antibodies to porcine parvovirus (ppv), aujeszky's disease virus (adv), porcine circovirus type 2 (pcv2), swine influenza virus, porcine respi ... | 2012 | 22247381 |
| prevalence of and risk factors associated with viral and bacterial pathogens in farmed european wild boar. | the aim of this study was to estimate in farmed european wild boars the prevalence of and risk factors associated with a range of common porcine viral and bacterial infections, namely, porcine parvovirus (ppv), porcine circovirus type 2 (pcv2), swine influenza virus (siv), aujeszky's disease virus (adv), classical swine fever virus (csfv), swine vesicular disease virus (svdv), coronavirus causing transmissible gastroenteritis (tgev), porcine reproductive and respiratory syndrome virus (prrsv), m ... | 2012 | 22516920 |
| development and laboratory evaluation of two lateral flow devices for the detection of vesicular stomatitis virus in clinical samples. | two lateral flow devices (lfd) for the detection of vesicular stomatitis (vs) virus (vsv), types indiana (vsv-ind) and new jersey (vsv-nj) were developed using monoclonal antibodies c1 and f25vsvnj-45 to the respective vsv serotypes. the performance of the lfds was evaluated in the laboratory on suspensions of vesicular epithelia and cell culture passage derived supernatants of vsv. the collection of test samples included 105 positive for vsv-ind (92 vesicular epithelial suspensions and 13 cell ... | 2011 | 22230813 |
| pan-serotypic detection of foot-and-mouth disease virus using a minor groove binder probe reverse transcription polymerase chain reaction assay. | a novel assay for the pan-serotypic detection of foot-and-mouth disease virus (fmdv) was designed using a 5' conjugated minor groove binder (mgb) probe real-time rt-pcr system. this assay targets the 3d region of the fmdv genome and is capable of detecting 20 copies of a transcribed rna standard. the linear range of the test was eight logs from 2 × 10¹ to 2 × 108 copies and amplification time was approximately 2 h. using a panel of 83 rna samples from representative fmdv isolates, the diagnostic ... | 2011 | 21419170 |
| differentiation of foot-and-mouth disease-infected pigs from vaccinated pigs using antibody-detecting sandwich elisa. | the presence of serum antibodies for nonstructural proteins of the foot-and-mouth disease virus (fmdv) can differentiate fmdv-infected animals from vaccinated animals. in this study, a sandwich elisa was developed for rapid detection of the foot-and-mouth disease (fmd) antibodies; it was based on an escherichia coli-expressed, highly conserved region of the 3abc nonstructural protein of the fmdv o/tw/99 strain and a monoclonal antibody derived from the expressed protein. the diagnostic sensitivi ... | 2011 | 21467761 |
| multiplex rt-pcr detection and microarray typing of vesicular disease viruses. | a vesicular disease multiplex reverse transcription (rt)-pcr with an accompanying microarray assay was developed for simultaneous detection and typing of foot-and-mouth disease virus (fmdv) and vesicular stomatitis virus (vsv), and for the detection of swine vesicular disease virus (svdv) and vesicular exanthema of swine virus (vesv). the multiplex rt-pcr successfully detected viral rna from a collection of 49 strains of vesicular viruses, including multiple strains from all seven serotypes of f ... | 2011 | 21620898 |
| virus inactivation by salt (nacl) and phosphate supplemented salt in a 3d collagen matrix model for natural sausage casings. | due to possible presence and spread of contagious animal viruses via natural sausage casings the international trade in these food products is subject to veterinary and public health requirements. in order to manage these restrictions we determined the effect of casing preservation on four highly contagious viruses for livestock: foot-and-mouth-disease virus (fmdv), classical swine fever virus (csfv), swine vesicular disease virus (svdv) and african swine fever virus (asfv). we used an in vitro ... | 2011 | 21632134 |
| rapid typing of foot-and-mouth disease serotype asia 1 by reverse transcription loop-mediated isothermal amplification. | a reverse transcriptase loop-mediated isothermal amplification (rt-lamp) assay was rapidly used to detect serotype asia 1 of foot-and-mouth disease virus (fmdv) within 45 min at 61°c. all fmdv serotype asia 1 reference strains were positive by rt-lamp, while other viruses such as fmdv serotypes o, c, a and classical swine fever virus, swine vesicular disease virus, porcine reproductive and respiratory syndrome virus and japanese encephalitis virus remained negative. furthermore, fmdv sreotype as ... | 2011 | 22040459 |
| Application of GP5 protein to develop monoclonal antibody against porcine reproductive and respiratory syndrome virus. | In this study, a panel of monoclonal antibodies (mAbs) against Porcine reproductive and respiratory syndrome virus(PRRSV), named as 8C9 and4B4, were produced by fusing SP2/0 myeloma cells and spleen cells of BALB/c mice immunized with the PRRSV (TCID(50)=5.5), screened by the indirect ELISA and subjected to several limiting dilutions. mAbs were then identified by biological characterization. Among the two fusion cell strains, 8C9 belonged to the IgG1 subclass and 4B4 belonged to the IgG2a subcla ... | 2011 | 21847758 |
| specificity of the coxsackievirus b4 vp4 capsid protein investigated in silico. | the enterovirus genus encompasses several species and various serotypes, like coxsackievirus-b1 (cv-b1) to cv-b6, and many variants. the role of these viruses, especially cv-b4, in the pathogenesis of type 1 diabetes is strongly suspected. it has been reported that antibodies directed towards the region of amino acids 11-30 of the vp4 capsid protein enhance the infection of human peripheral blood mononuclear cells with cv-b4. in order to predict the inter- and intra-serotype specificity of the r ... | 2011 | 21878192 |
| development and laboratory evaluation of a lateral flow device for the detection of swine vesicular disease virus in clinical samples. | a lateral flow device (lfd) for the detection of swine vesicular disease (svd) virus (svdv) and differential diagnosis from foot-and-mouth disease (fmd) was developed using a monoclonal antibody (mab c70). the performance of the lfd was evaluated in the laboratory on suspensions of vesicular epithelia and cell culture passage derived supernatants of svdv and porcine teschovirus (enterovirus; pev). the collection of test samples included 157 which were positive for svdv (84 vesicular epithelial s ... | 2010 | 19819260 |
| prevalence of antibodies to selected viral and bacterial pathogens in wild boar (sus scrofa) in campania region, italy. | serum samples were collected from wild boars (sus scrofa) harvested during the 2005-2006 hunting season in campania, southern italy. samples were tested for antibodies to leptospira interrogan, brucella spp., salmonella spp., aujeszky disease virus (adv), porcine reproductive and respiratory stress syndrome virus (prrsv), porcine parvovirus (ppv), classical swine fever virus (csfv), and swine vesicular disease virus (svdv). of the 342 serum samples tested, 15 (4.4%) were seropositive to brucella ... | 2010 | 20090052 |
| the development of a rapid sybr one step real-time rt-pcr for detection of porcine reproductive and respiratory syndrome virus. | prompt detection of prrsv in the field samples is important for effective prrs control, thereby reducing the potentially serious economic damage which can result from an outbreak. in this study, a rapid sybr-based, one step real-time rt-pcr quantitative reverse transcription pcr (qrt-pcr) has been developed for the detection of porcine reproductive and respiratory syndrome virus (prrsv). primers were designed based on the sequence of highly conservative region of prrsv n gene. | 2010 | 20459705 |
| [comparison of different elisas for detection of antibodies to swine vesicular disease virus in sera from experimentally infected animals]. | the study has shown the efficiency of a competitive elisa (c-elisa) variant or an indirect elisa (i-elusa) in the detection of antibodies to swine vesicular disease virus (svdv) versus traditional assays, such as a microneutralization test, a blocking elida test, and a the reference test ceditest svdv (cedi-diagnostics b.v., netherlands). specific antibodies in the pig sera could be detected by c-elisa on days 4-5 and by i-elisa on day 6 after experimental svdv infection. specific antibodies wer ... | 2010 | 20608082 |
| a survey of porcine picornaviruses and adenoviruses in fecal samples in spain. | in the course of an epidemiologic surveillance program for swine diseases carried out in spain, 206 cytopathic viruses were isolated from 600 porcine fecal samples between 2004 and 2005. the virus isolates were examined using reverse transcription polymerase chain reaction (rt-pcr) methods specific for different types of porcine picornaviruses, including members of the teschovirus, enterovirus, and sapelovirus genera, and pcr for porcine adenoviruses. of the 206 isolates, 97 (47%) were identifie ... | 2010 | 20807938 |
| [development of an indirect elisa for the detection of antibodies to swine vesicular disease virus during monitoring studies]. | an indirect elisa (i-elisa) has been developed for swine vesicular disease virus-specific antibody detection. the analytic sensitivity of i-elisa testing of serum samples from experimentally infected pigs with the known vn titer was 2 log2. its diagnostic specificity was demonstrated as 100% in 4485 swine serum samples from different regions of the russian federation. | 2010 | 20886713 |
| development of a minor groove binder assay for real-time one-step rt-pcr detection of swine vesicular disease virus. | the design and development of a 5' conjugated minor groove binder (mgb) probe real-time rt-pcr assay are described for rapid, sensitive and specific detection of swine vesicular disease virus (svdv) rna. the assay is designed to target the 2c gene of the svdv genome and is capable of detecting 2×10(2) copies of an rna standard per reaction. it does not detect any of the other rna viruses that cause vesicular disease in pigs, or the human enterovirus, coxsackie b5 virus (cvb5) which is closely re ... | 2010 | 21073902 |
| internalization of swine vesicular disease virus into cultured cells: a comparative study with foot-and-mouth disease virus. | we performed a comparative analysis of the internalization mechanisms used by three viruses causing important vesicular diseases in animals. swine vesicular disease virus (svdv) internalization was inhibited by treatments that affected clathrin-mediated endocytosis and required traffic through an endosomal compartment. svdv particles were found in clathrin-coated pits by electron microscopy and colocalized with markers of early endosomes by confocal microscopy. svdv infectivity was significantly ... | 2009 | 19225001 |
| virus interaction with the apical junctional complex. | in order to infect pathogens must breach the epithelial barriers that separate the organism from the external environment or that cover the internal cavities and ducts of the body. epithelia seal the passage through the paracellular pathway with the apical junctional complex integrated by tight and adherens junctions. in this review we describe how viruses like coxsackie, swine vesicular disease virus, adenovirus, reovirus, feline calcivirus, herpes viruses 1 and 2, pseudorabies, bovine herpes v ... | 2009 | 19273098 |
| enzyme-linked immunosorbent assay using glycoprotein and monoclonal antibody for detecting antibodies to vesicular stomatitis virus serotype new jersey. | in this study, an enzyme-linked immunosorbent assay (elisa) using glycoprotein and a monoclonal antibody (mab) was developed for the detection of antibodies to vesicular stomatitis virus (vsv) serotype new jersey (nj). the glycoprotein to be used as a diagnostic antigen was extracted from partially purified vsv-nj, and a neutralizing mab specific to vsv-nj was incorporated to compete with antibodies in a blocking elisa using glycoprotein (gp elisa). the cutoff of the gp elisa was set at 40% inhi ... | 2009 | 19279165 |
| application of real-time reverse transcription polymerase chain reaction for the detection of svdv. | application of real-time rt-pcr (rrt-pcr) for detection of swine vesicular disease virus (svdv) in samples of archival svdv isolates and clinical samples collected from svdv infected pigs was described. a primer set that targets the ires region of the svdv genome and taqman probe specific for a highly conserved region in svdv rna ires region were used. the assay detected viral rna in all tested archival strains of svdv isolated in europe during years 1972-73 and 1992 as well as in clinical sampl ... | 2009 | 19459449 |
| microarray-based detection of viruses causing vesicular or vesicular-like lesions in livestock animals. | definitive diagnosis of vesicular or vesicular-like lesions in livestock animals presents challenges both for veterinary clinicians and diagnostic laboratories. it is often impossible to diagnose the causative disease agent on a clinical basis alone and difficult to collect ample vesicular epithelium samples. due to restrictions of time and sample size, once laboratory tests have ruled out foot-and-mouth disease, vesicular stomatitis and swine vesicular disease a definitive diagnosis may remain ... | 2009 | 18621489 |
| antibodies to selected viral disease agents in wild boars from the czech republic. | blood samples were collected from wild boar (sus scrofa) shot during the hunting season from 1999 to 2005 in the czech republic. sera were tested by enzyme-linked immunosorbent assay for the presence of antibodies against classical swine fever virus (csfv), swine vesicular disease virus (svdv), aujeszky's disease virus (adv), and bovine viral diarrhea virus (bvdv). indirect fluorescence antibody test was used for detection of antibodies against porcine circovirus type 2 (pcv-2) and transmissible ... | 2008 | 18689671 |
| a one-step reverse transcriptase loop-mediated isothermal amplification assay for simple and rapid detection of swine vesicular disease virus. | this report describes the development of a one-step reverse transcriptase loop-mediated isothermal amplification (rt-lamp) assay for the detection of swine vesicular disease virus (svdv). the assay detects the virus rapidly, within 30-60 min and the result is visualised either by gel-electrophoresis or by the naked eye through the addition of sybrgreen. a collection of 28 svdv isolates were tested positive, while heterologous viruses such as foot-and-mouth disease virus and vesicular stomatitis ... | 2008 | 17920701 |
| rapid and differential diagnosis of foot-and-mouth disease, swine vesicular disease, and vesicular stomatitis by a new multiplex rt-pcr assay. | a highly sensitive and specific one-step multiplex rt-pcr assay has been developed and standardised for the simultaneous and differential detection of the most important vesicular viruses affecting livestock: foot-and-mouth disease virus (fmdv), swine vesicular disease virus (svdv), and vesicular stomatitis virus (vsv). the method uses three primer sets, each one specific for the corresponding virus, selected to detect of all serotypes of fmd and vs. the detection range was confirmed by examinat ... | 2008 | 17964668 |
| dynamics of picornavirus rna replication within infected cells. | replication of many picornaviruses is inhibited by low concentrations of guanidine. guanidine-resistant mutants are readily isolated and the mutations map to the coding region for the 2c protein. using in vitro replication assays it has been determined previously that guanidine blocks the initiation of negative-strand synthesis. we have now examined the dynamics of rna replication, measured by quantitative rt-pcr, within cells infected with either swine vesicular disease virus (an enterovirus) o ... | 2008 | 18198379 |
| diagnostic evaluation of multiplexed reverse transcription-pcr microsphere array assay for detection of foot-and-mouth and look-alike disease viruses. | a high-throughput multiplexed assay was developed for the differential laboratory detection of foot-and-mouth disease virus (fmdv) from viruses that cause clinically similar diseases of livestock. this assay simultaneously screens for five rna and two dna viruses by using multiplexed reverse transcription-pcr (mrt-pcr) amplification coupled with a microsphere hybridization array and flow-cytometric detection. two of the 17 primer-probe sets included in this multiplex assay were adopted from prev ... | 2008 | 18216216 |
| subcellular distribution of swine vesicular disease virus proteins and alterations induced in infected cells: a comparative study with foot-and-mouth disease virus and vesicular stomatitis virus. | the intracellular distribution of swine vesicular disease virus (svdv) proteins and the induced reorganization of endomembranes in ibrs-2 cells were analyzed. fluorescence to new svdv capsids appeared first upon infection, concentrated in perinuclear circular structures and colocalized to dsrna. as in foot-and-mouth disease virus (fmdv)-infected cells, a vesicular pattern was predominantly found in later stages of svdv capsid morphogenesis that colocalized with those of non-structural proteins 2 ... | 2008 | 18279902 |
| a laboratory study of survival of selected microorganisms after heat treatment of biowaste used in biogas plants. | the aim of the study was to assess the effect of pasteurisation, as set by the european regulation ec 1774/2002, on selected pathogens and indicator organisms. unpasteurised substrate (biowaste), including animal by-products from a full-scale biogas plant was heat treated under laboratory conditions at 70 degrees c and 55 degrees c for 30 min and 60 min. heat treatment at 55 degrees c for 60 min was not sufficient to achieve a hygienically acceptable product. heat treatment at 70 degrees c for 3 ... | 2008 | 18513960 |
| microarray-based molecular detection of foot-and-mouth disease, vesicular stomatitis and swine vesicular disease viruses, using padlock probes. | the world organization for animal health (office international des epizooties, oie) includes the diseases caused by foot-and-mouth disease virus (fmdv), swine vesicular disease virus (svdv), and vesicular stomatitis virus (vsv), as "diseases notifiable to the oie". foot-and-mouth disease (fmd) outbreaks have severe economical as well as social effects and cannot be differentiated from the diseases caused by the other two viruses on the basis of clinical symptoms. efficient laboratory techniques ... | 2007 | 17451815 |
| significance of arginine 20 in the 2a protease for swine vesicular disease virus pathogenicity. | pathogenic and attenuated strains of swine vesicular disease virus (svdv), an enterovirus, have been characterized previously and, by using chimeric infectious cdna clones, the key determinants of pathogenicity in pigs have been mapped to the coding region for 1d-2a. within this region, residue 20 of the 2a protease is particularly significant. inoculation of pigs with mutant viruses containing single amino acid substitutions at this residue leads to the appearance of revertants, often containin ... | 2007 | 17622632 |
| reappearance of swine vesicular disease virus in portugal. | 2007 | 17630426 | |
| development of a novel recombinant encapsidated rna particle: evaluation as an internal control for diagnostic rt-pcr. | this report describes the generation of novel encapsidated rna particles and their evaluation as in-tube internal controls in diagnostic real-time reverse-transcription pcr (rrt-pcr) assays for the detection of rna viruses. a cassette containing sequences of 2 diagnostic primer sets for foot-and-mouth disease virus (fmdv) and a set for swine vesicular disease virus (svdv) was engineered into a full-length cdna clone containing the rna-2 segment of cowpea mosaic virus (cpmv). after co-inoculation ... | 2007 | 17727966 |
| [construction and sequencing of full-length cdna clone of swine vesicular disease virus strain hk'1/70]. | by race, 2 overlapping cdna fragments (3'pcr and 5'pcr fragments) covering the full genome of swine vesicular disease virus strain hk'1/70 were amplified from total rna extracted from experimentally infected suckling mice. these fragments were cloned into pgem-t easy vector, respectively. 5'pcr fragment was digested by enzymes of aat ii and bssh ii, and the aat ii-bssh ii-digested 5'pcr fragment was obtained and cloned into the recombinant pgem-t easy vector containing 3'pcr fragment,the recombi ... | 2007 | 17886721 |
| a serological survey of selected pathogens in wild boar in slovenia. | serum samples collected from 178 shot wild boars (sus scrofa) were tested for the presence of antibodies against classical swine fever virus, aujeszky's disease virus (adv), porcine reproductive and respiratory syndrome virus, porcine respiratory coronavirus (prcv), transmissible gastroenteritis virus, swine influenza virus, porcine parvovirus (ppv), swine vesicular disease virus, actinobacillus pleuropneumoniae (app), mycoplasma hyopneumoniae, salmonella spp., brucella spp. and haemophilus para ... | 2006 | 16460352 |
| detection and serotype-specific differentiation of vesicular stomatitis virus using a multiplex, real-time, reverse transcription-polymerase chain reaction assay. | a multiplex, real-time reverse transcription-polymerase chain reaction (rt-pcr) assay was developed that allowed simultaneous detection and rapid differentiation of vesicular stomatitis virus strains--new jersey (vsv-nj) and indiana 1, 2, and 3 (vsv-in1-3). this assay involves use of a set of vsv universal primers located in the l gene that amplify vsv-in1-3 and vsv-nj using probes that allow differentiation of the major serotypes indiana and new jersey. the assay was evaluated using reference v ... | 2006 | 16617693 |
| development of a real-time pcr assay based on primer-probe energy transfer for the detection of swine vesicular disease virus. | a real-time pcr assay based on primer-probe energy transfer (priproet) was developed to detect swine vesicular disease virus (svdv). specificity tests of svdv and heterologous virus showed specific amplification of svdv strains only. the amplification plot for the closely related coxsackievirus b5 remained negative. the sensitivity of assay was five copies of viral genome equivalents. a key point of the assay is tolerance toward mutations in the probe region. melting curve analysis directly afte ... | 2006 | 16835700 |
| importance of arginine 20 of the swine vesicular disease virus 2a protease for activity and virulence. | a major virulence determinant of swine vesicular disease virus (svdv), an enterovirus that causes an acute vesicular disease, has been mapped to residue 20 of the 2a protease. the svdv 2a protease cleaves the 1d-2a junction in the viral polyprotein, induces cleavage of translation initiation factor eif4gi, and stimulates the activity of enterovirus internal ribosome entry sites (iress). the 2a protease from an attenuated strain of svdv (ile at residue 20) is significantly defective at inducing c ... | 2005 | 15596836 |
| viruses in boar semen: detection and clinical as well as epidemiological consequences regarding disease transmission by artificial insemination. | many viruses have been reported to be present in boar semen, particularly during the viremic phase of the diseases. some of them, such foot-and-mouth disease virus, porcine reproductive and respiratory syndrome virus, swine vesicular disease virus, porcine parvovirus, picornaviruses, adenoviruses, enteroviruses, japanese encephalitis virus, pseudorabies virus, african swine fever virus and reoviruses are of particular importance and accurate monitoring prior to and during the presence of boars i ... | 2005 | 15626416 |
| more recent swine vesicular disease virus isolates retain binding to coxsackie-adenovirus receptor, but have lost the ability to bind human decay-accelerating factor (cd55). | swine vesicular disease virus (svdv) evolved from coxsackie b virus serotype 5 (cvb5) in the recent past, crossing the species barrier from humans to pigs. here, svdv isolates from early and recent outbreaks have been compared for their capacity to utilize the progenitor virus receptors coxsackie-adenovirus receptor (car) and decay-accelerating factor (daf; cd55). virus titre of cvb5 and svdv isolates it'66 and uk'72 on human hela cells was reduced by pre-incubation with either anti-daf or anti- ... | 2005 | 15831949 |
| noninfectious virus-like particle antigen for detection of swine vesicular disease virus antibodies in pigs by enzyme-linked immunosorbent assay. | an inactivated svdv antigen is used in current enzyme-linked immunosorbent assays (elisas) for the detection of antibodies to swine vesicular disease virus (svdv). to develop a noninfectious recombinant alternative, we produced svdv-like particles (vlps) morphologically and antigenically resembling authentic svdv particles by using a dual baculovirus recombinant, which expresses simultaneously the p1 and 3cd protein genes of svdv under different promoters. antigenic differences between recombina ... | 2005 | 16085909 |
| sequence analysis of the 5' untranslated region of swine vesicular disease virus reveals block deletions between the end of the internal ribosomal entry site and the initiation codon. | swine vesicular disease virus (svdv) is a picornavirus closely related to the human pathogen coxsackievirus b5. in common with other picornaviruses, the 5' untranslated region (5' utr) of svdv contains an internal ribosomal entry site (ires) that plays an important role in cap-independent translation. the aim of this study was to use rt-pcr and sequencing to characterize a fragment of the 5' utr encompassing the entire ires. sequence analysis demonstrated high nucleotide identities within the ir ... | 2005 | 16186229 |
| molecular characterization of coxsackievirus b5 isolates. | coxsackie b viruses of serotype 5 are associated frequently with sporadic cases of neurological diseases, epidemics of meningitis, and chronic diseases such as cardiomyopathy and diabetes. in this article, 15 strains of coxsackievirus b5 isolated from patients with neurological disorders and healthy people were investigated by partial sequencing in the 5' half of the vp1 region and compared to other published sequences of coxsackievirus b5, in the same genomic region. all coxsackievirus b5 seque ... | 2004 | 14695669 |
| evaluation of real-time reverse transcription polymerase chain reaction assays for the detection of swine vesicular disease virus. | differential detection of swine vesicular disease virus (svdv) from the other vesicular disease viruses of foot-and-mouth disease (fmd), vesicular stomatitis (vs) and vesivirus is important as the vesicular lesions produced by these viruses are indistinguishable in pigs. two independent sets of primers and probe, designed from nucleotide sequences within the 5' untranslated region (utr) of the svdv genome, were evaluated in a real-time (5' nuclease probe-based or fluorogenic) pcr format. althoug ... | 2004 | 14738984 |
| heparan sulphate mediates swine vesicular disease virus attachment to the host cell. | heparan sulphate (hs) has been found to serve as receptor for initial cell binding of numerous viruses. different glycosaminoglycans (gags), including heparin and hs, were analysed for their ability to bind swine vesicular disease virus (svdv), a picornavirus with close homology to human coxsackie b5 virus. binding of svdv was established by heparin-affinity chromatography. in addition, infection of ib-rs-2 epithelial porcine cells was inhibited by treating the virus with soluble hs, heparin, an ... | 2004 | 14993651 |
| application of universal primers for identification of foot-and-mouth disease virus and swine vesicular disease virus by pcr and pcr-elisa. | two approaches for simultaneous identification of both foot-and-mouth disease virus (fmdv) and swine vesicular disease virus (svdv) are described: (1) a single-step reverse transcription-pcr with three primers and (2) a pcr-elisa assay with two universal primers for genome amplification and two virus-specific probes for identification. these methods are based on the use of 3d gene universal pcr primers, the structure of which was optimized and refined due to the close relationship between the tw ... | 2004 | 15168202 |
| detection of economically important viruses in boar semen by quantitative realtime pcr technology. | the objective of this study was to develop quantitative real-time polymerase chain reaction (reti-pcr) tests for the detection of five economically important viruses in swine semen namely, pseudorabies virus (prv), classical swine fever virus (csfv), foot-and-mouth disease virus (fmdv), swine vesicular disease virus (svdv), and porcine reproductive and respiratory syndrome virus (prrsv). each reti-pcr test was validated for specificity, analytical sensitivity (detection limits), and experimental ... | 2004 | 15288957 |
| use of automated real-time reverse transcription-polymerase chain reaction (rt-pcr) to monitor experimental swine vesicular disease virus infection in pigs. | automated real-time rt-pcr was evaluated as a diagnostic tool for swine vesicular disease virus (svdv) infection on a range of samples (vesicular epithelium, serum, nasal swabs, faeces) from four inoculated and three in-contact pigs over a period of 28 days. traditional diagnostic procedures (virus isolation, and elisas for antigen and antibody) were used in parallel. each inoculated pig developed a significant viraemia and clinical disease, and excreted virus, which was transmitted to the in-co ... | 2004 | 15511539 |
| [expression of antigenic region of vp1 gene of swine vesicular disease in escherichia coli]. | the antigenic region of vp1 gene of swine vesicular disease virus was amplified by reverse transcription polymerase chain reaction (rt-pcr) and nested polymerase chain reaction (npcr). after the amplified fragment was cloned into the expression vector pproex-htb. the insert position,the size and the reading frame of the insertion were identified by pcr, restriction digestion and sequence analysis of the recombinant plasmids. sds-page and western blot indicated that the transformed bl21(de3) by t ... | 2003 | 16279200 |
| establishment and characterization of two new pig cell lines for use in virological diagnostic laboratories. | two pig cell lines derived from kidney and trachea tissues and referred to as newborn swine kidney (nsk) and newborn pig trachea (nptr) were established following serial culture of primary cells. they were characterized by an epithelial-like morphology, high capacity to replicate and stability of the cell monolayer for several days after seeding. their modal chromosome number was modified in comparison to that of primary swine cells and they both displayed a transforming potential in vitro and d ... | 2003 | 12505635 |
| crystallization and preliminary x-ray analysis of swine vesicular disease virus (svdv). | three different crystal forms of the swine vesicular disease virus (svdv), isolate spa/2/'93, were obtained by the hanging-drop vapour-diffusion technique using ammonium sulfate and sodium/potassium phosphate as precipitants. monoclinic crystals, space group c2, with unit-cell parameters a = 473.7, b = 385.3, c = 472.8 a, beta = 100.4 degrees, contain one virus particle in the crystal asymmetric unit and diffract to 3.0 a resolution. a second type of crystals had a cubic morphology and diffracte ... | 2003 | 12595720 |
| crystal structure of swine vesicular disease virus and implications for host adaptation. | swine vesicular disease virus (svdv) is an enterovirus of the family picornaviridae that causes symptoms indistinguishable from those of foot-and-mouth disease virus. phylogenetic studies suggest that it is a recently evolved genetic sublineage of the important human pathogen coxsackievirus b5 (cbv5), and in agreement with this, it has been shown to utilize the coxsackie and adenovirus receptor (car) for cell entry. the 3.0-a crystal structure of strain uk/27/72 svdv (highly virulent) reveals th ... | 2003 | 12692248 |
| structure of swine vesicular disease virus: mapping of changes occurring during adaptation of human coxsackie b5 virus to infect swine. | the structure of swine vesicular disease virus (svdv) was solved and refined at a 3.0-a resolution by x-ray crystallography to gain information about the role of sequence changes that occurred as this virus evolved from the parental human pathogen coxsackievirus b5 (cvb5). these amino acid substitutions can be clustered in five distinct regions: (i) the antigenic sites, (ii) the hydrophobic pocket of the vp1 beta-sandwich, (iii) the putative car binding site, (iv) the putative heparan sulfate bi ... | 2003 | 12941886 |
| molecular analysis of echovirus 13 isolates and aseptic meningitis, spain. | echovirus 13 (ev13), considered rare, was reported worldwide in 2000, mostly related to aseptic meningitis outbreaks. in spain, 135 ev13 isolates were identified. the genetic relationships between 64 representative strains from spain and other reported isolates from the united states, germany, italy, japan, and sweden were described by analyzing the partial sequence of the major capsid protein (vp1) gene. the strains from spain were clearly identified as ev13 (79.5% similarity with the ev13 refe ... | 2003 | 12967490 |
| detection of porcine teschoviruses and enteroviruses by lightcycler real-time pcr. | porcine picornaviruses comprising at least 23 serotypes grouped into six species were described as causative agents of neurological disorders, reproductive failure, and aphthae-like dermal lesions of swine. other viruses such as classical swine fever virus (csfv), african swine fever virus, pseudorabies virus (prv), vesicular stomatitis virus, vesicular exanthema virus, porcine respiratory and reproductive syndrome virus, and porcine parvovirus (ppv) may cause diseases with similar clinical symp ... | 2003 | 14500127 |
| effects of mutations in the vp2/vp4 cleavage site of swine vesicular disease virus on rna encapsidation and viral infectivity. | we studied vp0 cleavage of swine vesicular disease virus (svdv), a member of the picornaviridae using a full-length cdna copy of the dutch svdv isolate. the influences of mutations, introduced at the cleavage site of svdv, on vp0 cleavage, rna encapsidation and viral infection were studied. double mutations at asparagine (vp0 aa 69) and serine (vp0 aa 70) resulted in no cleavage of vp0 and 100% inhibition of virus production. mutation of the asparagine into threonine or phenylalanine resulted in ... | 2003 | 14505087 |
| isotype specific elisas to detect antibodies against swine vesicular disease virus and their use in epidemiology. | isotype specific elisas to detect antibodies against swine vesicular disease, which may help to estimate the moment of infection, were developed and validated on sera from pigs experimentally infected with four different isolates of swine vesicular disease virus. virus specific igm antibodies could be detected from days 3-49 and occasionally up to day 91 after infection. igg1 antibodies were first detected at day 8 and igg2 at day 11. iga antibodies coincided with igg1 antibodies, but antibody t ... | 2002 | 12002546 |
| mapping of linear epitopes on the capsid proteins of swine vesicular disease virus using monoclonal antibodies. | the antigenic linear map of swine vesicular disease virus (svdv) has been studied using a repertoire of monoclonal antibodies (mabs) raised against a recombinant svdv polyprotein, p1. peptide-scanning analyses, cross-reactivity studies with homologous and heterologous viruses and predicted location on a computer-generated three-dimensional model of the capsid proteins have allowed the identification of five main linear sites. two sites, the n terminus of vp3 and amino acids 51-60 on vp1, corresp ... | 2002 | 12029154 |
| purification, crystallization and x-ray analysis of swine vesicular disease virus. | swine vesicular disease virus (svdv) is the etiological agent of swine vesicular disease, a highly contagious disease in pigs, and is related to coxsackie b virus. crystalline arrays of svdv can be observed in the cytoplasm of cells 4.5 h after inoculation to porcine kidney cells (ibrs-2 cells). crystals of the jx/78 strain of svdv were obtained from virus in two wells of crystallization conditions and present preliminary x-ray data to 3.6 a resolution. | 2002 | 12037316 |
| continuous porcine cell lines developed from alveolar macrophages: partial characterization and virus susceptibility. | porcine monomyeloid cell lines were established following transfection of primary porcine alveolar macrophage cultures with plasmid psv3neo, carrying genes for neomycin resistance and sv40 large t antigen. the parental clone 3d4 exhibited a relatively rapid doubling time (25.5 h), high plating efficiency and mixed phenotype with respect to growth on a solid support. single cell cloning of the 3d4 parent resulted in establishment of several cell lines; three of them designated 3d4/2, 3d4/21 and 3 ... | 2002 | 12088830 |
| characterization of neutralization sites on the circulating variant of swine vesicular disease virus (svdv): a new site is shared by svdv and the related coxsackie b5 virus. | using a panel of new monoclonal antibodies (mabs), five neutralizing, conformation-dependent sites have been identified on the antigenic variant of swine vesicular disease virus (svdv) circulating currently. in studies on the antigenic conservation of these sites, the four antigenic/genetic groups of svdv described showed distinguishable patterns, confirming this classification. by sequencing mab-resistant mutants, the five sites have been mapped precisely and localized on a three-dimensional mo ... | 2002 | 11752698 |
| a serodiagnostic elisa using recombinant antigen of swine transmissible gastroenteritis virus nucleoprotein. | a serodiagnostic elisa utilizing the recombinant nucleoprotein (rn protein) of transmissible gastroenteritis virus (tgev) was developed, and evaluated by examining a panel of 141 virus neutralization (vn) positive and 101 negative sera. the rn protein-based elisa (rnelisa) appeared to be highly sensitive and specific (98.6% and 98.0%, respectively) when it was compared to the vn test. the result was similar to that of an elisa based on purified viral antigens with showing good correlation (r=0.8 ... | 2001 | 11767065 |
| [sero-monitoring of notifiable diseases in wild boar in the netherlands 1999-2001]. | within the framework of a sero-monitoring system, in operation since 1996. blood samples from wild boar shot during the hunting seasons 1999-2000 and 2000-2001 in the netherlands were screened for the presence of antibodies against classical swine fever virus (csfv), swine vesicular disease virus (svdv), and anjeszky's disease virus (adv). the results indicate that csfv, svdv, and adv are uncommon in the wild boar population in the netherlands. because of the recent foot-and-mouth disease (fmd) ... | 2001 | 11780256 |
| deletion or substitution of the aphthovirus 3' ncr abrogates infectivity and virus replication. | the 3' noncoding region (ncr) of the genomic picornaviral rna is believed to contain major cis-acting signals required for negative-strand rna synthesis. the 3' ncr of foot-and-mouth disease virus (fmdv) was studied in the context of a full-length infectious clone in which the genetic element was deleted or exchanged for the equivalent region of a distantly related swine picornavirus, swine vesicular disease virus (svdv). deletion of the 3' ncr, while maintaining the intact poly(a) tail as well ... | 2001 | 11125162 |
| the n-terminal region of the vp1 protein of swine vesicular disease virus contains a neutralization site that arises upon cell attachment and is involved in viral entry. | the n-terminal region of vp1 of swine vesicular disease virus (svdv) is highly antigenic in swine, despite its internal location in the capsid. here we show that antibodies to this region can block infection and that allowing the virus to attach to cells increases this blockage significantly. the results indicate that upon binding to the cell, svdv capsid undergoes a conformational change that is temperature independent and that exposes the n terminus of vp1. this process makes this region acces ... | 2001 | 11134318 |
| persistent infection is a rare sequel following infection of pigs with swine vesicular disease virus. | nine isolates from pigs persistently infected with a recent italian isolate of swine vesicular disease (svd) virus, itl/9/93, were collected sequentially over 121 days and were characterized antigenically and biochemically. there was an accumulation of amino acid (aa) substitutions in the capsid proteins throughout the carrier state that could be correlated with alterations in antigenicity in virus isolates collected late stage in infection. the aa substitutions detected mainly occurred in vpi a ... | 2001 | 11561966 |
| virulence of swine vesicular disease virus is determined at two amino acids in capsid protein vp1 and 2a protease. | to identify the genetic determinants of virulence for swine vesicular disease virus, a panel of recombinant and site-directed mutant viruses were constructed from cdna clones of a virulent j1'73 strain and an avirulent h/3'76 strain. initial studies mapped the genetic determinants of virulence to either or both of the two sites at nucleotide (nt) 2842, encoding vp1-132, and nt 3355, encoding 2a-20. to determine their relative importance with regard to virulence, viruses mutated at either of thes ... | 2001 | 11597755 |
| an attenuating mutation in the 2a protease of swine vesicular disease virus, a picornavirus, regulates cap- and internal ribosome entry site-dependent protein synthesis. | virulent and avirulent strains of swine vesicular disease virus (svdv), a picornavirus, have been characterized previously. the major determinants for attenuation have been mapped to specific residues in the 1d-2a-coding region. the properties of the 2a proteases from the virulent and avirulent strains of svdv have now been examined. both proteases efficiently cleaved the 1d/2a junction in vitro and in vivo. however, the 2a protease of the avirulent strain of svdv was much less effective than th ... | 2001 | 11602706 |
| swine vesicular disease virus. pathology of the disease and molecular characteristics of the virion. | swine vesicular disease is a highly contagious disease of pigs that is caused by an enterovirus of the family picornaviridae. the virus is a relatively recent derivative of the human coxsackievirus b5, with which it has high molecular and antigenic homology. the disease is not severe, and affected animals usually show moderate general weakening and slight weight loss that is recovered in few days, as well as vesicular lesions in the mucosa of the mouth and nose and in the interdigital spaces of ... | 2000 | 11708597 |
| nucleotide sequences and mutations of the 5'-nontranslated region (5'ntr) of natural isolates of an epidemic echovirus 11' (prime). | an echovirus 11' (prime) virus caused an epidemic in hungary in 1989. the leading clinical form of the diseases was myocarditis. hemorrhagic hepatitis syndroms were also caused, however, with lethal outcome in 13 newborn babies. altogether 386 children suffered from registered clinical disease. no accumulation of serous meningitis cases and intrauterine death were observed during the epidemic, and the monovalent oral poliovirus vaccination campaign has prevented the further circulation of the vi ... | 2000 | 11205106 |
| [serosurveillance of notifiable veterinary diseases in wild boar in the netherlands]. | during the hunting season 1996-1999, blood samples were collected from wild boar shot in the netherlands. sera were screened for presence of antibodies against classical swine fever virus (csfv), swine vesicular disease virus (svdv), aujeszky's disease virus (adv), and trichinella spiralis. the results indicate that csfv, svdv, and adv are uncommon in the wild boar population. therefore, it seems that csfv, svdv, and adv infection in the wild boar population is not an important reservoir in the ... | 2000 | 10666784 |
| effects of chlorine, iodine, and quaternary ammonium compound disinfectants on several exotic disease viruses. | the effects of three representative disinfectants, chlorine (sodium hypochlorite), iodine (potassium tetraglicine triiodide), and quaternary ammonium compound (didecyldimethylammonium chloride), on several exotic disease viruses were examined. the viruses used were four enveloped viruses (vesicular stomatitis virus, african swine fever virus, equine viral arteritis virus, and porcine reproductive and respiratory syndrome virus) and two non-enveloped viruses (swine vesicular disease virus (svdv) ... | 2000 | 10676896 |
| outbreak of viral gastroenteritis due to sewage-contaminated drinking water. | in august 1998, a large outbreak of gastroenteritis occurred in a swiss village of 3500 inhabitants whereof more than 50% were affected. a high contamination of drinking water with faecal coliforms revealed a defect in the waste water system. the objective of the present study was to investigate the outbreak in respect of the presence of human pathogenic viruses. drinking water and clinical samples from patients were examined for the presence of 'norwalk-like viruses' (nlvs) and enteroviruses. n ... | 2000 | 10746582 |
| molecular analysis of the prototype coxsackievirus b5 genome. | to facilitate studies of the phylogenetic relationship between enteroviruses, in particular the prototype strain (faulkner) of coxsackievirus b5 (cvb5f) and other cvb5 isolates and to facilitate studies of the interactions between cvb5f and the target cell, the complete nucleotide sequence of the prototype has been determined. the complete sequence was collected from three overlapping reverse transcription polymerase chain reaction (rt-pcr) generated amplicons. molecular analysis of the cvb5f ge ... | 2000 | 10752549 |
| immune recognition of swine vesicular disease virus structural proteins: novel antigenic regions that are not exposed in the capsid. | swine vesicular disease virus (svdv) is an enterovirus of the picornaviridae family that belongs to the coxsackievirus b group. a number of antigenic sites have been identified in svdv by analysis of neutralizing monoclonal antibody-resistant mutants and shown to be exposed on the surface of the capsid. in this paper we have identified seven new immunodominant antigenic regions in svdv capsid proteins by a peptide scanning method, using a panel of sera from infected pigs. when these antigenic re ... | 2000 | 10772981 |
| the coxsackie-adenovirus receptor (car) is used by reference strains and clinical isolates representing all six serotypes of coxsackievirus group b and by swine vesicular disease virus. | group b coxsackieviruses are etiologically linked to many human diseases, and cell surface receptors are postulated to play an important role in mediating their pathogenesis. the coxsackievirus adenovirus receptor (car) has been shown to function as a receptor for selected strains of coxsackievirus group b (cvb) serotypes 3, 4, and 5 and is postulated to serve as a receptor for all six serotypes. in this study, we demonstrate that car can serve as a receptor for laboratory reference strains and ... | 2000 | 10814575 |
| detection of porcine enteroviruses by nrt-pcr: differentiation of cpe groups i-iii with specific primer sets. | porcine enteroviruses (pev) comprising at least 13 serotypes grouped into three species are described as causative agents of neurological disorders, fertility disorders, and dermal lesions of swine. despite their well-documented acid stability, enteric infection route, and similarity of clinical symptoms, most of the porcine enterovirus (pev) serotypes are set apart from the genus enterovirus of the picornaviridae. hence, pcr procedures used commonly to detect enteroviruses are not applicable to ... | 2000 | 10960708 |
| singleton reactors in the diagnosis of swine vesicular disease: the role of coxsackievirus b5. | swine vesicular disease virus (svdv) and coxsackie b5 virus (cvb5) are closely related viruses that can infect swine and man and give rise to cross-reacting serum antibodies. it is, therefore, possible that svd antibodies found in serologic screenings of pigs are induced by cvb5. single positive animals found in screening programmes are generally referred to as singleton reactors (sr). to determine whether sr in svdv screenings are induced by cvb5 infection, virus neutralisation tests (vnts) and ... | 2000 | 10973703 |
| primary diagnosis of foot-and-mouth disease by reverse transcription polymerase chain reaction. | universal and serotype-specific primer sets were used in simple reverse transcription polymerase chain reaction (rt-pcr) assays on field samples of epithelium and vesicular fluid to determine their suitability for primary diagnosis of all seven serotypes of foot-and-mouth disease (fmd). the specificity of reactions was confirmed by using other vesicular disease viruses, namely: swine vesicular disease virus, vesicular stomatitis virus and three vesiviruses. this resulted in the identification of ... | 2000 | 10996650 |
| construction of a full-length infectious cdna clone of swine vesicular disease virus strain net/1/92 and analysis of new antigenic variants derived from it. | the dutch swine vesicular disease virus (svdv) isolate net/1/92 was one of the first isolates belonging to a new svdv antigenic group. this strain was completely sequenced and was shown to have 93% similarity with the ukg/27/72 isolate. to enable antigenicity, replication, maturation and pathogenicity studies of net/1/92, an infectious full-length cdna clone, designated psvd146, was prepared. the in vitro and in vivo biological properties of the virus derived from psvd146 were studied by analysi ... | 2000 | 11038390 |
| sero-surveillance of wild boar in the netherlands, 1996-1999. | from 1996 to 1999, blood samples were collected from wild boar shot during the hunting season in crown properties, national parks and the free wildlife belt in the netherlands. sera were screened for the presence of antibodies against classical swine fever virus (csfv), swine vesicular disease virus (svdv), aujeszky's disease virus (adv) and trichinella spiralis. the results of the sero-surveillance system indicate that csfv, svdv and adv are uncommon within the wild boar population. hence, the ... | 2000 | 11107628 |
| heterotypic inhibition of foot-and-mouth disease virus infection by combinations of rna transcripts corresponding to the 5' and 3' regions. | strategies to inhibit rna virus multiplication based on the use of interfering nucleic acids have to consider the high genetic polymorphism exhibited by this group of viruses. here, we report high levels of heterotypic inhibition of foot-and-mouth disease virus (fmdv) infective particle formation in cotransfection experiments of susceptible cell lines with infections viral rna and combinations of viral transcripts. the interfering molecules used include the following regions on type c fmdv rna: ... | 1999 | 10669263 |
| highly sensitive detection of swine vesicular disease virus based on a single tube rt-pcr system and dig-elisa detection. | a highly sensitive detection of swine vesicular disease virus (svdv) based on a single tube rt-pcr system and digoxigenin (dig)-pcr-elisa detection was developed. using a one tube rt-pcr system, optimisation of the pcr conditions and optimisation of the microwell hybridisation and colourimetric detection of the amplicons resulted in a method that could detect viral rna in infected tissue culture fluid with a titre as low as 0.1 tcid50/100 microl. the same sensitivity was obtained with svdv-spike ... | 1999 | 10029329 |
| identification of neutralizing epitopes on a european strain of swine vesicular disease virus. | six neutralizing monoclonal antibodies (mabs) were used to isolate mab neutralization-resistant (mar) mutants from a recent european strain of swine vesicular disease virus (svdv), itl/9/93. sequencing of mar mutants identified two epitopes located at positions analogous to sites 2a (vp2) and 3b (vp3) on poliovirus (pv) which have been previously identified on a japanese strain of svdv. a third epitope near to the c terminus of vp1, not previously recognized on svdv, was tentatively identified i ... | 1999 | 10073685 |
| mapping the genetic determinants of pathogenicity and plaque phenotype in swine vesicular disease virus. | a series of recombinant viruses were constructed using infectious cdna clones of the virulent j1'73 (large plaque phenotype) and the avirulent h/3'76 (small plaque phenotype) strains of swine vesicular disease virus to identify the genetic determinants of pathogenicity and plaque phenotype. both traits could be mapped to the region between nucleotides (nt) 2233 and 3368 corresponding to the c terminus of vp3, the whole of vp1, and the n terminus of 2a. in this region, there are eight nucleotide ... | 1999 | 10074117 |
| molecular evolution of swine vesicular disease virus. | phylogenetic analysis was used to examine the evolutionary relationships within a group of coxsackie b viruses that contained representatives of the major serotypes of this group and 45 isolates of swine vesicular disease virus (svdv) from asia and europe. separate analyses of sequence data from two regions of the viral genomes encoding the vp1 and 3bc genes both revealed that the svdv belonged to a single monophyletic group which could be clearly distinguished from all other sampled coxsackievi ... | 1999 | 10092004 |
| laboratory-scale inactivation of african swine fever virus and swine vesicular disease virus in pig slurry. | two methods were evaluated for the inactivation of african swine fever (asv) and swine vesicular disease (svd) viruses in pig slurry: chemical treatment and heat treatment. the addition of naoh or ca(oh)2 at different concentration/time combinations at 4 degrees c and 22 degrees c was examined, as was virus stability at different temperature/time combinations. asf virus (asfv) was less resistant to both methods than svd virus (svdv). in slurry from one source, asfv was inactivated at 65 degrees ... | 1999 | 10432596 |
| the complete consensus sequence of coxsackievirus b6 and generation of infectious clones by long rt-pcr. | the full length sequence for the human pathogen coxsackievirus b6 (cvb6, schmitt strain) has been determined. we used long rt-pcr to generate full length dna amplicon of cvb6, and then directly sequenced the amplicons. one-step cloning of the full length amplicon enabled us to obtain an infectious clone of cvb6. rna generated from cvb6 amplicon dna or cvb6 clones, by transcription with t7 rna polymerase, was demonstrated to be infectious upon transfection into hela cells in vitro. the cvb6 genom ... | 1999 | 10500285 |
| recovery and assay of african swine fever and swine vesicular disease viruses from pig slurry. | assaying samples for infectious virus is more difficult when the sample is toxic to cells used in the assay, e.g. with samples of infected pig slurry. various techniques were compared for the recovery of african swine fever virus (asfv) and swine vesicular disease virus (svdv) in pig slurry. extraction with freon led to 80-100% recovery of svdv added to pig slurry. the assay sensitivity enabled undiluted, centrifuged sample to be put directly onto monolayers of ib-rs2 cells, allowing a minimum d ... | 1999 | 10540248 |
| pilot scale thermal treatment of pig slurry for the inactivation of animal virus pathogens. | this paper describes a pilot scale treatment plant that has been designed and built for the thermal inactivation in pig slurry of two viruses that infect pigs--african swine fever virus (asfv) and swine vesicular disease virus (svdv). the plant treats pig slurry continuously at a rate of up to 100 litres/hour and functions by heating the slurry, maintaining at least 99.99% of the slurry at the required temperature for a minimum period of 5 minutes, and then recovering the heat to raise the tempe ... | 1999 | 10565423 |
| reduction of singleton reactors against swine vesicular disease virus by a combination of virus neutralisation test, monoclonal antibody-based competitive elisa and isotype specific elisa. | pigs which are serologically positive for swine vesicular disease virus (svdv) but which show no clinical signs and for which there is neither a relevant history of the disease on the holding nor contact with a known outbreak are considered as singleton reactors. false positive serological results for an epizootic disease, like svd, in a non-vaccinated population or in imported animals are of great concern to international trade. for the virus neutralisation test, the gold standard for svd, sing ... | 1998 | 9506808 |
| evolution of a common structural core in the internal ribosome entry sites of picornavirus. | the translational control involving internal ribosome binding occurs in poliovirus (pv), human rhinoviruses (hrv), encephalomyocarditis virus (emcv), foot-and-mouth disease virus (fmdv), and hepatitis a virus (hav). internal ribosome binding utilizes cis-acting genetic elements of approximately 450 nucleotides (nt) termed the internal ribosome entry sites (ires) found in these picornaviral 5'-untranslated region (5'utr). although these ires elements are quite different in their primary sequence, ... | 1998 | 9562889 |
| an international comparative analysis of a competitive elisa for the detection of antibodies to swine vesicular disease virus. | 1998 | 9683084 | |
| viruses produced from complementary dna of virulent and avirulent strains of swine vesicular disease viruses retain the in vivo and in vitro characteristics of the parental strain. | a full-length cdna copy of the genome of the pathogenic strain, j1'73, of swine vesicular disease virus (svdv) was constructed and inserted into the plasmid psvl to generate a recombinant plasmid psvlsj1. infectious virus was produced following transfection of cultured mammalian cells with the plasmid. the recovered virus had the same in vitro properties as the parental strain with regard to antigenicity, plaque size on ibrs-2 cells and single-step growth. pigs were experimentally infected with ... | 1998 | 9687864 |
| a rt-pcr assay for the differential diagnosis of vesicular viral diseases of swine. | a rt-pcr assay based on specific amplification of rna sequences from each of the etiological agents of three important vesicular diseases that affect swine, foot-and-mouth disease virus (fmdv), swine vesicular disease virus (svdv), and vesicular stomatitis virus (vsv), was developed. genotype-specific primers that amplified dna fragments of differential size from svdv 3d gene or vsv l gene were selected with the aid of a computer program. experimental testing of the primers predicted as svdv-spe ... | 1998 | 9694330 |
| validation of a monoclonal antibody-based elisa to detect antibodies directed against swine vesicular disease virus. | a simple, rapid and sensitive competitive monoclonal antibody-based elisa for the detection of antibodies directed against swine vesicular disease virus (svdv) was developed. the elisa was validated using field sera originating from svdv-infected and non-infected dutch pig herds, reference sera obtained from the community reference laboratory for swine vesicular disease at the institute for animal health, pirbright laboratory, uk, and sera from animals infected experimentally. when testing 4277 ... | 1998 | 9820579 |
| the persistence of swine vesicular disease virus infection in pigs. | two groups of pigs were infected with a recent italian isolate of swine vesicular disease virus (svdv). blood, nasal swabs and faeces were collected for up to 6 months after exposure to infection and animals were killed at regular intervals to obtain tissues post-mortem. these samples were examined for virus by conventional means and for viral rna (vrna) by reverse transcription-nested polymerase chain reaction (rt-npcr). virus was identified intermittently from both clinically and subclinically ... | 1998 | 9825800 |
| molecular cloning, expression and immunological analysis of the capsid precursor polypeptide (p1) from swine vesicular disease virus. | swine vesicular disease virus (svdv) is the aetiological agent of a highly contagious viral disease of pigs, whose symptoms are indistinguishable from those caused by foot-and-mouth disease virus (fmdv). the gene coding for the capsid protein precursor of svdv (p1) from a recent spanish isolate (spa/1/'93) was cloned and expressed in bacteria, and the antigenicity and immunogenicity of the recombinant product were evaluated. the recombinant p1 was recognised by antibodies against svdv induced in ... | 1998 | 9870584 |
| detection of swine vesicular disease virus rna by reverse transcription-polymerase chain reaction. | two polymerase chain reaction (pcr) assays are described for the detection of swine vesicular disease virus (svdv) rna, a reverse transcription pcr (rt-pcr) and a reverse transcription nested pcr (rt-npcr). both the rt-pcr and rt-npcr were able to detect representative members of each of seven phylogenetically distinct groups of svdv and gave negative results with a range of porcine enteroviruses and of viruses responsible for vesicular conditions in pigs. when combined with a commercial kit for ... | 1997 | 9128868 |
| effects of quaternary ammonium compounds with 0.1% sodium hydroxide on swine vesicular disease virus. | the effects of quaternary ammonium compounds (qacs) with sodium hydroxide on swine vesicular disease virus (svdv), an enterovirus were studied. didecyldimethylammonium chloride (ddac) with 0.1% naoh showed a stronger effect against svdv than other qacs with 0.1% naoh. the effect of ddac with 0.1% naoh was strong at 40 degrees c. ddac was effective against svdv at ph values around 11.0, but not in the distilled water control. the effect of ddac with 0.1% naoh was already observed at 1 min after m ... | 1997 | 9192351 |