Publications

crystal structure of swine vesicular disease virus and implications for host adaptation.	swine vesicular disease virus (svdv) is an enterovirus of the family picornaviridae that causes symptoms indistinguishable from those of foot-and-mouth disease virus. phylogenetic studies suggest that it is a recently evolved genetic sublineage of the important human pathogen coxsackievirus b5 (cbv5), and in agreement with this, it has been shown to utilize the coxsackie and adenovirus receptor (car) for cell entry. the 3.0-a crystal structure of strain uk/27/72 svdv (highly virulent) reveals th ...	2003	12692248
evaluation of real-time reverse transcription polymerase chain reaction assays for the detection of swine vesicular disease virus.	differential detection of swine vesicular disease virus (svdv) from the other vesicular disease viruses of foot-and-mouth disease (fmd), vesicular stomatitis (vs) and vesivirus is important as the vesicular lesions produced by these viruses are indistinguishable in pigs. two independent sets of primers and probe, designed from nucleotide sequences within the 5' untranslated region (utr) of the svdv genome, were evaluated in a real-time (5' nuclease probe-based or fluorogenic) pcr format. althoug ...	2004	14738984
heparan sulphate mediates swine vesicular disease virus attachment to the host cell.	heparan sulphate (hs) has been found to serve as receptor for initial cell binding of numerous viruses. different glycosaminoglycans (gags), including heparin and hs, were analysed for their ability to bind swine vesicular disease virus (svdv), a picornavirus with close homology to human coxsackie b5 virus. binding of svdv was established by heparin-affinity chromatography. in addition, infection of ib-rs-2 epithelial porcine cells was inhibited by treating the virus with soluble hs, heparin, an ...	2004	14993651
application of universal primers for identification of foot-and-mouth disease virus and swine vesicular disease virus by pcr and pcr-elisa.	two approaches for simultaneous identification of both foot-and-mouth disease virus (fmdv) and swine vesicular disease virus (svdv) are described: (1) a single-step reverse transcription-pcr with three primers and (2) a pcr-elisa assay with two universal primers for genome amplification and two virus-specific probes for identification. these methods are based on the use of 3d gene universal pcr primers, the structure of which was optimized and refined due to the close relationship between the tw ...	2004	15168202
detection of economically important viruses in boar semen by quantitative realtime pcr technology.	the objective of this study was to develop quantitative real-time polymerase chain reaction (reti-pcr) tests for the detection of five economically important viruses in swine semen namely, pseudorabies virus (prv), classical swine fever virus (csfv), foot-and-mouth disease virus (fmdv), swine vesicular disease virus (svdv), and porcine reproductive and respiratory syndrome virus (prrsv). each reti-pcr test was validated for specificity, analytical sensitivity (detection limits), and experimental ...	2004	15288957
molecular characterization of coxsackievirus b5 isolates.	coxsackie b viruses of serotype 5 are associated frequently with sporadic cases of neurological diseases, epidemics of meningitis, and chronic diseases such as cardiomyopathy and diabetes. in this article, 15 strains of coxsackievirus b5 isolated from patients with neurological disorders and healthy people were investigated by partial sequencing in the 5' half of the vp1 region and compared to other published sequences of coxsackievirus b5, in the same genomic region. all coxsackievirus b5 seque ...	2004	14695669
use of automated real-time reverse transcription-polymerase chain reaction (rt-pcr) to monitor experimental swine vesicular disease virus infection in pigs.	automated real-time rt-pcr was evaluated as a diagnostic tool for swine vesicular disease virus (svdv) infection on a range of samples (vesicular epithelium, serum, nasal swabs, faeces) from four inoculated and three in-contact pigs over a period of 28 days. traditional diagnostic procedures (virus isolation, and elisas for antigen and antibody) were used in parallel. each inoculated pig developed a significant viraemia and clinical disease, and excreted virus, which was transmitted to the in-co ...	2004	15511539
importance of arginine 20 of the swine vesicular disease virus 2a protease for activity and virulence.	a major virulence determinant of swine vesicular disease virus (svdv), an enterovirus that causes an acute vesicular disease, has been mapped to residue 20 of the 2a protease. the svdv 2a protease cleaves the 1d-2a junction in the viral polyprotein, induces cleavage of translation initiation factor eif4gi, and stimulates the activity of enterovirus internal ribosome entry sites (iress). the 2a protease from an attenuated strain of svdv (ile at residue 20) is significantly defective at inducing c ...	2005	15596836
more recent swine vesicular disease virus isolates retain binding to coxsackie-adenovirus receptor, but have lost the ability to bind human decay-accelerating factor (cd55).	swine vesicular disease virus (svdv) evolved from coxsackie b virus serotype 5 (cvb5) in the recent past, crossing the species barrier from humans to pigs. here, svdv isolates from early and recent outbreaks have been compared for their capacity to utilize the progenitor virus receptors coxsackie-adenovirus receptor (car) and decay-accelerating factor (daf; cd55). virus titre of cvb5 and svdv isolates it'66 and uk'72 on human hela cells was reduced by pre-incubation with either anti-daf or anti- ...	2005	15831949
noninfectious virus-like particle antigen for detection of swine vesicular disease virus antibodies in pigs by enzyme-linked immunosorbent assay.	an inactivated svdv antigen is used in current enzyme-linked immunosorbent assays (elisas) for the detection of antibodies to swine vesicular disease virus (svdv). to develop a noninfectious recombinant alternative, we produced svdv-like particles (vlps) morphologically and antigenically resembling authentic svdv particles by using a dual baculovirus recombinant, which expresses simultaneously the p1 and 3cd protein genes of svdv under different promoters. antigenic differences between recombina ...	2005	16085909
sequence analysis of the 5' untranslated region of swine vesicular disease virus reveals block deletions between the end of the internal ribosomal entry site and the initiation codon.	swine vesicular disease virus (svdv) is a picornavirus closely related to the human pathogen coxsackievirus b5. in common with other picornaviruses, the 5' untranslated region (5' utr) of svdv contains an internal ribosomal entry site (ires) that plays an important role in cap-independent translation. the aim of this study was to use rt-pcr and sequencing to characterize a fragment of the 5' utr encompassing the entire ires. sequence analysis demonstrated high nucleotide identities within the ir ...	2005	16186229
viruses in boar semen: detection and clinical as well as epidemiological consequences regarding disease transmission by artificial insemination.	many viruses have been reported to be present in boar semen, particularly during the viremic phase of the diseases. some of them, such foot-and-mouth disease virus, porcine reproductive and respiratory syndrome virus, swine vesicular disease virus, porcine parvovirus, picornaviruses, adenoviruses, enteroviruses, japanese encephalitis virus, pseudorabies virus, african swine fever virus and reoviruses are of particular importance and accurate monitoring prior to and during the presence of boars i ...	2005	15626416
a serological survey of selected pathogens in wild boar in slovenia.	serum samples collected from 178 shot wild boars (sus scrofa) were tested for the presence of antibodies against classical swine fever virus, aujeszky's disease virus (adv), porcine reproductive and respiratory syndrome virus, porcine respiratory coronavirus (prcv), transmissible gastroenteritis virus, swine influenza virus, porcine parvovirus (ppv), swine vesicular disease virus, actinobacillus pleuropneumoniae (app), mycoplasma hyopneumoniae, salmonella spp., brucella spp. and haemophilus para ...	2006	16460352
detection and serotype-specific differentiation of vesicular stomatitis virus using a multiplex, real-time, reverse transcription-polymerase chain reaction assay.	a multiplex, real-time reverse transcription-polymerase chain reaction (rt-pcr) assay was developed that allowed simultaneous detection and rapid differentiation of vesicular stomatitis virus strains--new jersey (vsv-nj) and indiana 1, 2, and 3 (vsv-in1-3). this assay involves use of a set of vsv universal primers located in the l gene that amplify vsv-in1-3 and vsv-nj using probes that allow differentiation of the major serotypes indiana and new jersey. the assay was evaluated using reference v ...	2006	16617693
development of a real-time pcr assay based on primer-probe energy transfer for the detection of swine vesicular disease virus.	a real-time pcr assay based on primer-probe energy transfer (priproet) was developed to detect swine vesicular disease virus (svdv). specificity tests of svdv and heterologous virus showed specific amplification of svdv strains only. the amplification plot for the closely related coxsackievirus b5 remained negative. the sensitivity of assay was five copies of viral genome equivalents. a key point of the assay is tolerance toward mutations in the probe region. melting curve analysis directly afte ...	2006	16835700
microarray-based molecular detection of foot-and-mouth disease, vesicular stomatitis and swine vesicular disease viruses, using padlock probes.	the world organization for animal health (office international des epizooties, oie) includes the diseases caused by foot-and-mouth disease virus (fmdv), swine vesicular disease virus (svdv), and vesicular stomatitis virus (vsv), as "diseases notifiable to the oie". foot-and-mouth disease (fmd) outbreaks have severe economical as well as social effects and cannot be differentiated from the diseases caused by the other two viruses on the basis of clinical symptoms. efficient laboratory techniques ...	2007	17451815
development of a novel recombinant encapsidated rna particle: evaluation as an internal control for diagnostic rt-pcr.	this report describes the generation of novel encapsidated rna particles and their evaluation as in-tube internal controls in diagnostic real-time reverse-transcription pcr (rrt-pcr) assays for the detection of rna viruses. a cassette containing sequences of 2 diagnostic primer sets for foot-and-mouth disease virus (fmdv) and a set for swine vesicular disease virus (svdv) was engineered into a full-length cdna clone containing the rna-2 segment of cowpea mosaic virus (cpmv). after co-inoculation ...	2007	17727966
significance of arginine 20 in the 2a protease for swine vesicular disease virus pathogenicity.	pathogenic and attenuated strains of swine vesicular disease virus (svdv), an enterovirus, have been characterized previously and, by using chimeric infectious cdna clones, the key determinants of pathogenicity in pigs have been mapped to the coding region for 1d-2a. within this region, residue 20 of the 2a protease is particularly significant. inoculation of pigs with mutant viruses containing single amino acid substitutions at this residue leads to the appearance of revertants, often containin ...	2007	17622632
reappearance of swine vesicular disease virus in portugal.		2007	17630426
[construction and sequencing of full-length cdna clone of swine vesicular disease virus strain hk'1/70].	by race, 2 overlapping cdna fragments (3'pcr and 5'pcr fragments) covering the full genome of swine vesicular disease virus strain hk'1/70 were amplified from total rna extracted from experimentally infected suckling mice. these fragments were cloned into pgem-t easy vector, respectively. 5'pcr fragment was digested by enzymes of aat ii and bssh ii, and the aat ii-bssh ii-digested 5'pcr fragment was obtained and cloned into the recombinant pgem-t easy vector containing 3'pcr fragment,the recombi ...	2007	17886721
antibodies to selected viral disease agents in wild boars from the czech republic.	blood samples were collected from wild boar (sus scrofa) shot during the hunting season from 1999 to 2005 in the czech republic. sera were tested by enzyme-linked immunosorbent assay for the presence of antibodies against classical swine fever virus (csfv), swine vesicular disease virus (svdv), aujeszky's disease virus (adv), and bovine viral diarrhea virus (bvdv). indirect fluorescence antibody test was used for detection of antibodies against porcine circovirus type 2 (pcv-2) and transmissible ...	2008	18689671
a one-step reverse transcriptase loop-mediated isothermal amplification assay for simple and rapid detection of swine vesicular disease virus.	this report describes the development of a one-step reverse transcriptase loop-mediated isothermal amplification (rt-lamp) assay for the detection of swine vesicular disease virus (svdv). the assay detects the virus rapidly, within 30-60 min and the result is visualised either by gel-electrophoresis or by the naked eye through the addition of sybrgreen. a collection of 28 svdv isolates were tested positive, while heterologous viruses such as foot-and-mouth disease virus and vesicular stomatitis ...	2008	17920701
rapid and differential diagnosis of foot-and-mouth disease, swine vesicular disease, and vesicular stomatitis by a new multiplex rt-pcr assay.	a highly sensitive and specific one-step multiplex rt-pcr assay has been developed and standardised for the simultaneous and differential detection of the most important vesicular viruses affecting livestock: foot-and-mouth disease virus (fmdv), swine vesicular disease virus (svdv), and vesicular stomatitis virus (vsv). the method uses three primer sets, each one specific for the corresponding virus, selected to detect of all serotypes of fmd and vs. the detection range was confirmed by examinat ...	2008	17964668
dynamics of picornavirus rna replication within infected cells.	replication of many picornaviruses is inhibited by low concentrations of guanidine. guanidine-resistant mutants are readily isolated and the mutations map to the coding region for the 2c protein. using in vitro replication assays it has been determined previously that guanidine blocks the initiation of negative-strand synthesis. we have now examined the dynamics of rna replication, measured by quantitative rt-pcr, within cells infected with either swine vesicular disease virus (an enterovirus) o ...	2008	18198379
diagnostic evaluation of multiplexed reverse transcription-pcr microsphere array assay for detection of foot-and-mouth and look-alike disease viruses.	a high-throughput multiplexed assay was developed for the differential laboratory detection of foot-and-mouth disease virus (fmdv) from viruses that cause clinically similar diseases of livestock. this assay simultaneously screens for five rna and two dna viruses by using multiplexed reverse transcription-pcr (mrt-pcr) amplification coupled with a microsphere hybridization array and flow-cytometric detection. two of the 17 primer-probe sets included in this multiplex assay were adopted from prev ...	2008	18216216
subcellular distribution of swine vesicular disease virus proteins and alterations induced in infected cells: a comparative study with foot-and-mouth disease virus and vesicular stomatitis virus.	the intracellular distribution of swine vesicular disease virus (svdv) proteins and the induced reorganization of endomembranes in ibrs-2 cells were analyzed. fluorescence to new svdv capsids appeared first upon infection, concentrated in perinuclear circular structures and colocalized to dsrna. as in foot-and-mouth disease virus (fmdv)-infected cells, a vesicular pattern was predominantly found in later stages of svdv capsid morphogenesis that colocalized with those of non-structural proteins 2 ...	2008	18279902
a laboratory study of survival of selected microorganisms after heat treatment of biowaste used in biogas plants.	the aim of the study was to assess the effect of pasteurisation, as set by the european regulation ec 1774/2002, on selected pathogens and indicator organisms. unpasteurised substrate (biowaste), including animal by-products from a full-scale biogas plant was heat treated under laboratory conditions at 70 degrees c and 55 degrees c for 30 min and 60 min. heat treatment at 55 degrees c for 60 min was not sufficient to achieve a hygienically acceptable product. heat treatment at 70 degrees c for 3 ...	2008	18513960
microarray-based detection of viruses causing vesicular or vesicular-like lesions in livestock animals.	definitive diagnosis of vesicular or vesicular-like lesions in livestock animals presents challenges both for veterinary clinicians and diagnostic laboratories. it is often impossible to diagnose the causative disease agent on a clinical basis alone and difficult to collect ample vesicular epithelium samples. due to restrictions of time and sample size, once laboratory tests have ruled out foot-and-mouth disease, vesicular stomatitis and swine vesicular disease a definitive diagnosis may remain ...	2009	18621489
enzyme-linked immunosorbent assay using glycoprotein and monoclonal antibody for detecting antibodies to vesicular stomatitis virus serotype new jersey.	in this study, an enzyme-linked immunosorbent assay (elisa) using glycoprotein and a monoclonal antibody (mab) was developed for the detection of antibodies to vesicular stomatitis virus (vsv) serotype new jersey (nj). the glycoprotein to be used as a diagnostic antigen was extracted from partially purified vsv-nj, and a neutralizing mab specific to vsv-nj was incorporated to compete with antibodies in a blocking elisa using glycoprotein (gp elisa). the cutoff of the gp elisa was set at 40% inhi ...	2009	19279165
internalization of swine vesicular disease virus into cultured cells: a comparative study with foot-and-mouth disease virus.	we performed a comparative analysis of the internalization mechanisms used by three viruses causing important vesicular diseases in animals. swine vesicular disease virus (svdv) internalization was inhibited by treatments that affected clathrin-mediated endocytosis and required traffic through an endosomal compartment. svdv particles were found in clathrin-coated pits by electron microscopy and colocalized with markers of early endosomes by confocal microscopy. svdv infectivity was significantly ...	2009	19225001
virus interaction with the apical junctional complex.	in order to infect pathogens must breach the epithelial barriers that separate the organism from the external environment or that cover the internal cavities and ducts of the body. epithelia seal the passage through the paracellular pathway with the apical junctional complex integrated by tight and adherens junctions. in this review we describe how viruses like coxsackie, swine vesicular disease virus, adenovirus, reovirus, feline calcivirus, herpes viruses 1 and 2, pseudorabies, bovine herpes v ...	2009	19273098
application of real-time reverse transcription polymerase chain reaction for the detection of svdv.	application of real-time rt-pcr (rrt-pcr) for detection of swine vesicular disease virus (svdv) in samples of archival svdv isolates and clinical samples collected from svdv infected pigs was described. a primer set that targets the ires region of the svdv genome and taqman probe specific for a highly conserved region in svdv rna ires region were used. the assay detected viral rna in all tested archival strains of svdv isolated in europe during years 1972-73 and 1992 as well as in clinical sampl ...	2009	19459449
[development of an indirect elisa for the detection of antibodies to swine vesicular disease virus during monitoring studies].	an indirect elisa (i-elisa) has been developed for swine vesicular disease virus-specific antibody detection. the analytic sensitivity of i-elisa testing of serum samples from experimentally infected pigs with the known vn titer was 2 log2. its diagnostic specificity was demonstrated as 100% in 4485 swine serum samples from different regions of the russian federation.	2010	20886713
development of a minor groove binder assay for real-time one-step rt-pcr detection of swine vesicular disease virus.	the design and development of a 5' conjugated minor groove binder (mgb) probe real-time rt-pcr assay are described for rapid, sensitive and specific detection of swine vesicular disease virus (svdv) rna. the assay is designed to target the 2c gene of the svdv genome and is capable of detecting 2×10(2) copies of an rna standard per reaction. it does not detect any of the other rna viruses that cause vesicular disease in pigs, or the human enterovirus, coxsackie b5 virus (cvb5) which is closely re ...	2010	21073902
development and laboratory evaluation of a lateral flow device for the detection of swine vesicular disease virus in clinical samples.	a lateral flow device (lfd) for the detection of swine vesicular disease (svd) virus (svdv) and differential diagnosis from foot-and-mouth disease (fmd) was developed using a monoclonal antibody (mab c70). the performance of the lfd was evaluated in the laboratory on suspensions of vesicular epithelia and cell culture passage derived supernatants of svdv and porcine teschovirus (enterovirus; pev). the collection of test samples included 157 which were positive for svdv (84 vesicular epithelial s ...	2010	19819260
prevalence of antibodies to selected viral and bacterial pathogens in wild boar (sus scrofa) in campania region, italy.	serum samples were collected from wild boars (sus scrofa) harvested during the 2005-2006 hunting season in campania, southern italy. samples were tested for antibodies to leptospira interrogan, brucella spp., salmonella spp., aujeszky disease virus (adv), porcine reproductive and respiratory stress syndrome virus (prrsv), porcine parvovirus (ppv), classical swine fever virus (csfv), and swine vesicular disease virus (svdv). of the 342 serum samples tested, 15 (4.4%) were seropositive to brucella ...	2010	20090052
the development of a rapid sybr one step real-time rt-pcr for detection of porcine reproductive and respiratory syndrome virus.	prompt detection of prrsv in the field samples is important for effective prrs control, thereby reducing the potentially serious economic damage which can result from an outbreak. in this study, a rapid sybr-based, one step real-time rt-pcr quantitative reverse transcription pcr (qrt-pcr) has been developed for the detection of porcine reproductive and respiratory syndrome virus (prrsv). primers were designed based on the sequence of highly conservative region of prrsv n gene.	2010	20459705
[comparison of different elisas for detection of antibodies to swine vesicular disease virus in sera from experimentally infected animals].	the study has shown the efficiency of a competitive elisa (c-elisa) variant or an indirect elisa (i-elusa) in the detection of antibodies to swine vesicular disease virus (svdv) versus traditional assays, such as a microneutralization test, a blocking elida test, and a the reference test ceditest svdv (cedi-diagnostics b.v., netherlands). specific antibodies in the pig sera could be detected by c-elisa on days 4-5 and by i-elisa on day 6 after experimental svdv infection. specific antibodies wer ...	2010	20608082
a survey of porcine picornaviruses and adenoviruses in fecal samples in spain.	in the course of an epidemiologic surveillance program for swine diseases carried out in spain, 206 cytopathic viruses were isolated from 600 porcine fecal samples between 2004 and 2005. the virus isolates were examined using reverse transcription polymerase chain reaction (rt-pcr) methods specific for different types of porcine picornaviruses, including members of the teschovirus, enterovirus, and sapelovirus genera, and pcr for porcine adenoviruses. of the 206 isolates, 97 (47%) were identifie ...	2010	20807938
pan-serotypic detection of foot-and-mouth disease virus using a minor groove binder probe reverse transcription polymerase chain reaction assay.	a novel assay for the pan-serotypic detection of foot-and-mouth disease virus (fmdv) was designed using a 5' conjugated minor groove binder (mgb) probe real-time rt-pcr system. this assay targets the 3d region of the fmdv genome and is capable of detecting 20 copies of a transcribed rna standard. the linear range of the test was eight logs from 2 × 10¹ to 2 × 108 copies and amplification time was approximately 2 h. using a panel of 83 rna samples from representative fmdv isolates, the diagnostic ...	2011	21419170
differentiation of foot-and-mouth disease-infected pigs from vaccinated pigs using antibody-detecting sandwich elisa.	the presence of serum antibodies for nonstructural proteins of the foot-and-mouth disease virus (fmdv) can differentiate fmdv-infected animals from vaccinated animals. in this study, a sandwich elisa was developed for rapid detection of the foot-and-mouth disease (fmd) antibodies; it was based on an escherichia coli-expressed, highly conserved region of the 3abc nonstructural protein of the fmdv o/tw/99 strain and a monoclonal antibody derived from the expressed protein. the diagnostic sensitivi ...	2011	21467761
multiplex rt-pcr detection and microarray typing of vesicular disease viruses.	a vesicular disease multiplex reverse transcription (rt)-pcr with an accompanying microarray assay was developed for simultaneous detection and typing of foot-and-mouth disease virus (fmdv) and vesicular stomatitis virus (vsv), and for the detection of swine vesicular disease virus (svdv) and vesicular exanthema of swine virus (vesv). the multiplex rt-pcr successfully detected viral rna from a collection of 49 strains of vesicular viruses, including multiple strains from all seven serotypes of f ...	2011	21620898
virus inactivation by salt (nacl) and phosphate supplemented salt in a 3d collagen matrix model for natural sausage casings.	due to possible presence and spread of contagious animal viruses via natural sausage casings the international trade in these food products is subject to veterinary and public health requirements. in order to manage these restrictions we determined the effect of casing preservation on four highly contagious viruses for livestock: foot-and-mouth-disease virus (fmdv), classical swine fever virus (csfv), swine vesicular disease virus (svdv) and african swine fever virus (asfv). we used an in vitro ...	2011	21632134
rapid typing of foot-and-mouth disease serotype asia 1 by reverse transcription loop-mediated isothermal amplification.	a reverse transcriptase loop-mediated isothermal amplification (rt-lamp) assay was rapidly used to detect serotype asia 1 of foot-and-mouth disease virus (fmdv) within 45 min at 61°c. all fmdv serotype asia 1 reference strains were positive by rt-lamp, while other viruses such as fmdv serotypes o, c, a and classical swine fever virus, swine vesicular disease virus, porcine reproductive and respiratory syndrome virus and japanese encephalitis virus remained negative. furthermore, fmdv sreotype as ...	2011	22040459
Application of GP5 protein to develop monoclonal antibody against porcine reproductive and respiratory syndrome virus.	In this study, a panel of monoclonal antibodies (mAbs) against Porcine reproductive and respiratory syndrome virus(PRRSV), named as 8C9 and4B4, were produced by fusing SP2/0 myeloma cells and spleen cells of BALB/c mice immunized with the PRRSV (TCID(50)=5.5), screened by the indirect ELISA and subjected to several limiting dilutions. mAbs were then identified by biological characterization. Among the two fusion cell strains, 8C9 belonged to the IgG1 subclass and 4B4 belonged to the IgG2a subcla ...	2011	21847758
specificity of the coxsackievirus b4 vp4 capsid protein investigated in silico.	the enterovirus genus encompasses several species and various serotypes, like coxsackievirus-b1 (cv-b1) to cv-b6, and many variants. the role of these viruses, especially cv-b4, in the pathogenesis of type 1 diabetes is strongly suspected. it has been reported that antibodies directed towards the region of amino acids 11-30 of the vp4 capsid protein enhance the infection of human peripheral blood mononuclear cells with cv-b4. in order to predict the inter- and intra-serotype specificity of the r ...	2011	21878192
development and laboratory evaluation of two lateral flow devices for the detection of vesicular stomatitis virus in clinical samples.	two lateral flow devices (lfd) for the detection of vesicular stomatitis (vs) virus (vsv), types indiana (vsv-ind) and new jersey (vsv-nj) were developed using monoclonal antibodies c1 and f25vsvnj-45 to the respective vsv serotypes. the performance of the lfds was evaluated in the laboratory on suspensions of vesicular epithelia and cell culture passage derived supernatants of vsv. the collection of test samples included 105 positive for vsv-ind (92 vesicular epithelial suspensions and 13 cell ...	2011	22230813
prevalence of and risk factors associated with viral and bacterial pathogens in farmed european wild boar.	the aim of this study was to estimate in farmed european wild boars the prevalence of and risk factors associated with a range of common porcine viral and bacterial infections, namely, porcine parvovirus (ppv), porcine circovirus type 2 (pcv2), swine influenza virus (siv), aujeszky's disease virus (adv), classical swine fever virus (csfv), swine vesicular disease virus (svdv), coronavirus causing transmissible gastroenteritis (tgev), porcine reproductive and respiratory syndrome virus (prrsv), m ...	2012	22516920
prevalence of antibodies to selected viral pathogens in wild boars (sus scrofa) in croatia in 2005-06 and 2009-10.	we determined prevalence of antibody to selected viral pathogens important for domestic pigs and livestock in 556 wild boar (sus scrofa) sera collected during 2005-06 and 2009-10 in four counties in croatia. these counties account for an important part of the croatian commercial pig production and have a high density of wild boars. samples were tested for antibodies to porcine parvovirus (ppv), aujeszky's disease virus (adv), porcine circovirus type 2 (pcv2), swine influenza virus, porcine respi ...	2012	22247381