Publications

giant cell formation in the brain of suckling mice infected with mouse hepatitis virus, jhm strain.	after intranasal inoculation with a neurotropic mouse hepatitis virus, jhm strain, suckling mice less than 2 weeks of age died in 4 or 5 days postinoculation, while those 3 weeks or more of age survived. in the brain lesions of mice 8 days or less of age, there appeared two different types of multinucleated giant cells. one of them which was irregular in shape with many virus particles, appeared mostly in the subependymal or perivascular areas after 72 h postinoculation and it seemed to have res ...	1979	226760
pathogenesis of mouse hepatitis virus infection. the role of nasal epithelial cells as a primary target of low-virulence virus, mhv-s.		1979	224286
pathology of diarrhea due to mouse hepatitis virus in the infant mouse.	two-day-old mice were inoculated orally with a mouse hepatitis virus which had been isolated from a suckling mouse with fatal diarrhea, and a systemic infection was shown to be established, though enterotropism of the isolate was evident. significant viral growth and syncytium forming lesions were first detectable in the intestines at day 2 postinoculation, followed by the development of focal necrotic hepatitis. the virus titer in both liver and intestine attained maximum levels at days 4 to 5. ...	1979	224229
new strain of mouse hepatitis virus as the cause of lethal enteritis in infant mice.	a new strain of mouse hepatitis virus (mhv) was isolated from pooled gut suspensions from an epizootic of lethal enteritis in newborn mice. negative-contrast electron microscopy showed an abundance of coronavirus particles in the intestinal contents and intestinal epithelium of moribund mice. we found no other virus in the epizootic. dams seroconverted to mhv polyvalent antigen and to the agent isolated, but did not develop antibodies to other known mouse pathogens. virus propagated in nctc-1469 ...	1979	222687
induction of lytic plaques by murine leukemia virus in murine sarcoma virus-transformed nonproducer mouse cells persistently infected with mouse hepatitis virus mhv-s.	kirsten murine sarcoma virus-transformed, nonproducer balb3t3 (k-balb) cells were persistently infected with mouse hepatitis virus, mhv-s. the cultures developed plaques after infection with murine leukemia viruses. if the murine leukemia virus-infected cultures were further submitted to the uv-xc assay, comparable numbers of xc plaques were obtained. the sensitivity to murine leukemia viruses, as determined by the uv-xc assay, was higher in mhv-s-infected cells as compared to uninfected k-balb ...	1979	218897
[effects of silver carbon on eliminating mouse hepatitis virus in water (author's transl)].		1978	215805
hepatosplenic myelosis in naturally occurring mouse hepatitis virus infection in the nude mouse.		1978	214604
in vitro growth characteristics and heterogeneity of mouse hepatitis virus type 3.	the in vitro virus yield of mhv3 reached 10(7) pfu/ml in mouse dbt cells infected with a virus suspension in hepes-buffered medium containing deae-dextran. the virus titer was 10(6) pfu/ml in the presence of 10 micrograms actinomycin d/ml mhv3 growth in dbt cells gave three peaks of density (1.10--1.14 g/cm3, 1.18--1.20 g/cm3, and 1.25--1.31 g/cm3) in sucrose gradients. all these peaks retained infectivity.	1978	211989
virus infection in demyelinating diseases.	several animal and human demyelinating diseases of the central nervous system (cns) are associated with rna or dna viruses. these viruses infect cns cells lytically or persistently. they mainly belong to the group of envelope viruses which derive their envelope partly from the host cell membrane. the process of virus release may result in the appearance of new antigens of virus-infected cells or the incorporation of cell membrane material into the viral envelope. these changes may lead to an imm ...	1978	211825
rna of mouse hepatitis virus.	the rna of mouse hepatitis virus, a coronavirus, was isolated from the virus released early in the infection and analyzed by sucrose gradient sedimentation and electrophoresis. it was found to consist of a piece of single-stranded rna of about 60s. its molecular weight was estimated to be 5.4 x 10(6) by electrophoresis in methylmercury-agarose gels. at least one third of the rna contained polyadenylated sequences. it is, therefore, probably positive stranded. the virus harvested late in the infe ...	1978	207885
serologic study on the prevalence of murine viruses in five canadian mouse colonies.	a serologic study was made of five canadian mouse colonies to determine the prevalence of antibodies to 11 murine viruses. a total of 139 sera from the five colonies were evaluated by complement fixation or hemagglutination inhibition methods. viral antibodies were present in all five colonies. antibodies to pneumonia virus of mice, minute virus of mice, theiler's encephalomyelitis virus, and reovirus type 3 were found in all five colonies. k-virus antibodies were present in four colonies. polyo ...	1977	201796
respiratory infection in mice with sialodacryoadenitis virus, a coronavirus of rats.	sialodacryoadenitis virus (sdav), a coronavirus of rats, evoked both serum neutralization and complement fixation antibody responses when inoculated intranasally in mice. weanling gnotobiotic cd-1 mice inoculated intranasally with 10(3.0) mean tissue culture infective doses of sdav remained asymptomatic. virus was recovered from the nasopharynx, trachea, and lung from day 2 to day 7. viral antigen was readily detected by indirect immunofluorescence in the lung but rarely in the nasopharynx. infe ...	1977	201568
naturally occurring mouse hepatitis virus infection in the nude mouse.	a group of athymic nude mice developed an unusual chronic wasting disease within 1-3 months after their arrival into the laboratory. affected nude mice had severe, acute-to-chronic, active hepatitis with multinucleated giant hepatocytes and fibrosis. vascular and central nervous system lesions were frequently present, giant cell peritonitis, ascites, and multinucleated giant cells in the intestinal epithelial villi were less frequently observed. mouse hepatitis virus was isolated from the livers ...	1977	195136
age-dependent response of mice to a mouse hepatitis virus, mhv-s.	to a mouse hepatitis virus strain mhv-s, 4 week-old icr mice were shown to be fully resistant irrespective of route and dose of inoculation, and fatal infection was produced only with cortisone treatment. two-week-old mice also showed high resistance to mhv-s except for after intracerebral inoculation, and 60% of infected mice died. mice aged 1 week or less, however, died after intracerebral, intraperitoneal, subcutaneous and intranasal inoculation, while some of them survived after peroral inoc ...	1977	194073
an improved method for titration of mouse hepatitis virus type 3 in a mouse cell culture.		1976	189731
response of nude mice to a mouse hepatitis virus isolated from a wasting nude mouse.	chronic active hepatitis was produced in nude mice after inoculation with a mouse hepatitis virus from a natural nude case which was incapable of causing fatal infection in haired heterozygous mice without cortisone. survival time of infected nude mice varied greatly from 1 to 10 weeks after the inoculation. degeneration and necrosis of hepatocytes, apparition of small and megalocytic hepatocytes, and infiltration of lymphocytes and plasma cells with fibrosis, were most remarkable in those which ...	1976	180312
particles morphologically resembling mouse hepatitis virus in nude mouse uterus.	particles morphologically resembling mouse hepatitis virus were observed in uterine gland lumens and endometrial cells of 8 nude mice. although 3 mice had been experimentally inoculated with baboon type c viral-infected materials, no ultrastructural evidence of oncornavirus-associated structures was observed.	1975	179967
carrier state of antibody and viruses in a mouse breeding colony persistently infected with sendai and mouse hepatitis viruses.	in a large-scale mouse breeder colony persistently infected with sendai and mouse hepatitis viruses, most adult breeders 8 wk or more of age were shown to have antibodies to both viruses when monitored over a periof of 20 mo. antibody to sendai virus, apparently transmitted from the dam, was detected in 76% and 2% of mice aged 3 and 4 wk. respectively, and 64% and 100% of mice aged 6 and 8 wk, respectively. by seroconversion of sentinel cage-mates, a sendai virus-carrier state was demonstrated w ...	1976	178959
macrophages genetically resistant to mouse hepatitis virus converted in vitro to susceptible macrophages.	genetic resistance to mouse hepatitis, which resides largely in the macrophages of resistant c3h mice, may be altered by exposing the cells in vitro to fluid from allogeneic mixed lymphocytes. a 1,000-fold increase in susceptibility was produced in these genetically resistant cells by exposure to this fluid. this presumed lymphokine was effective without producing any change in host adaption of the virus.	1976	175127
immunopathology of mouse hepatitis virus type 3 infection. iii. clinical and virologic observation of a persistent viral infection.	three types of viral sensitivity were observed in various mouse strains upon mhv3 infection: resistance, full susceptibility, and semisusceptibility. in the latter type, seen in several inbred strains including c3h, approximately 50% of the adult injected animals resisted to the acute disease. most of the surviving mice, however, developed a chronic disease with a wasting syndrome and occurrence of paralysis. the chronic period of the disease was characterized by a persistent viral infection, si ...	1975	168262
congenic strains of mice susceptible and resistant to mouse hepatitis virus.	a congenic strain of c3hss mice, which is histocompatible with c3h mice but differs from them in susceptibility to mouse hepatitis virus (mhv), has been developed by introducing the gene for susceptibility to the mhv-pri virus from the pri mice. this was accomplished by continual back-crossing of the hybrids to the c3h mice, but at the same time by selection of susceptibility by use of macrophage culture tests. after 20 back-crosses, a strain homozygous for susceptibility was produced by brother ...	1976	135262
antigenic relationship of the feline infections peritonitis virus to coronaviruses of other species.	utilizing the direct and indirect fluorescent antibody procedure, the antigenic relationship of the feline infectious peritonitis virus (fipv) to 7 other human and animal coronaviruses was studied. fipv was found to be closely related to transmissible gastroenteritis virus (tgev) of swine. transmissible gastroenteritis virus and fipv were in turn antigenically related to human coronavirus 229e (hcv-229e) and canine coronavirus (ccv). an interesting finding in the study was that the 8 coronavirus ...	1978	81044