Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the nature of mouse hepatitis virus infection in weanling mice. | 1955 | 13243199 | |
| the enhancing effect of murine hepatitis virus on the cerebral activity of pleuropneumonia-like organisms in mice. | pleuropneumoma-like organisms (pplo) of the catarrhal type were isolated from the brain of a swiss mouse during the cranial passage of mouse hepatitis virus-mhv(c). cranial injection of the pplo alone in swiss and princeton weanlings was attended by a meagre growth of the organisms in the brain, with no pathologic change. the growth of both catarrhal and conjunctival strains of pplo in the brains of swiss mice was greatly enhanced by the simultaneous injection of mhv(c). rolling was not a charac ... | 1957 | 13449230 |
| [influence of diisopropylammonium dichloroacetate (diedi) in experimental mhv-3 virus (mouse hepatitis virus) infection]. | 1960 | 13781410 | |
| [new isolation of a mouse hepatitis virus (edp virus). its virological biological characteristics]. | 1960 | 14419911 | |
| multiplication and cytopathogenicity of mouse hepatitis virus in mouse cell cultures. | 1961 | 14476514 | |
| [metabolic aspects of hepatic tissue in mhv-3 virus infections of the mouse. ii. gama-glycerophosphate-dehydrogenase and pyruvic-kinase]. | 1961 | 14494229 | |
| plaque formation by a mouse hepatitis virus. | 1962 | 13951824 | |
| mouse hepatitis virus infection as a highly contagious, prevalent, enteric infection of mice. | 1963 | 13982823 | |
| potentiating effect of k-virus on mouse hepatitis virus (mhv-s) in weanling mice. | 1963 | 14120346 | |
| an electron microscope study of the development of a mouse hepatitis virus in tissue culture cells. | samples taken at different intervals of time from suspension cultures of the nctc 1469 line of mouse liver-derived (ml) cells infected with a mouse hepatitis virus have been studied with the electron microscope. the experiments revealed that the viruses are incorporated into the cells by viropexis within 1 hour after being added to the culture. an increasing number of particles are found later inside dense cytoplasmic corpuscles similar to lysosomes. in the cytoplasm of the cells from the sample ... | 1965 | 14286297 |
| deoxyribonuclease i and ii activities in liver and spleen of mice experimentally infected by mouse hepatitis virus. | 1965 | 4285647 | |
| [changes in the atp content in liver tissue of the mouse in experimental virus mhv-3-hepatitis]. | 1966 | 4288710 | |
| [histochemical studies of the nucleic acids of the hepatic tissue of the mouse during a mhv-3 virus hepatitis. (study by using fluorescent staining with acridine orange)]. | 1966 | 4173062 | |
| studies on the pancreatitis in a mouse hepatitis virus (mhv-3) infection. | 1967 | 4296949 | |
| [preliminary observations on the influence of acute ccl-4 poisoning on experimental mouse hepatitis caused by mhv-3 virus]. | 1967 | 4297834 | |
| enhancing effect of the murine sarcoma virus (msv) on the replication of the mouse hepatitis virus (mhv) in vitro. | 1969 | 4306053 | |
| presence of particles other than the australia-sh antigen in a case of chronic active hepatitis with cirrhosis. | examination of serum specimens from a patient with chronic active hepatitis proved negative to the australia-sh antigen by immunodiffusion and by complement fixation. because the sera were anticomplementary they were examined with the electron microscope, and virus-like structures similar to members of the coronavirus group were identified. the possible significance of this finding in human serum and its relation to mouse hepatitis virus are discussed.a sample of the serum pool containing the ms ... | 1970 | 4313591 |
| isolation of a latent murine hepatitis virus from cultured mouse liver cells. | 1972 | 4344757 | |
| effect of cyclophosphamide on the genetic resistance of c 3 h mice to mouse hepatitis virus. | 1973 | 4348090 | |
| [sero-epidemiological observation on mouse hepatitis virus in a mouse breeder colony (author's transl)]. | 1973 | 4366446 | |
| detection of alpha-fetoprotein in mice infected with mouse hepatitis virus. | 1974 | 4373603 | |
| replication and plaque formation of mouse hepatitis virus (mhv-2) in mouse cell line dbt culture. | 1974 | 4365902 | |
| particles morphologically resembling mouse hepatitis virus in nude mouse uterus. | particles morphologically resembling mouse hepatitis virus were observed in uterine gland lumens and endometrial cells of 8 nude mice. although 3 mice had been experimentally inoculated with baboon type c viral-infected materials, no ultrastructural evidence of oncornavirus-associated structures was observed. | 1975 | 179967 |
| lactobacillus sp. isolates from mouse livers: saprophyte or potential pathogen? | over a 3-year sampling period lactobacillus sp. has been isolated from the livers of barrier-maintained and conventional mice. serology has failed to detect presence of mouse hepatitis virus antibodies in any of these mice. histopathological examination revealed only mild fatty change and small monocytic infiltrates. | 1975 | 806745 |
| immunopathology of mouse hepatitis virus type 3 infection. iii. clinical and virologic observation of a persistent viral infection. | three types of viral sensitivity were observed in various mouse strains upon mhv3 infection: resistance, full susceptibility, and semisusceptibility. in the latter type, seen in several inbred strains including c3h, approximately 50% of the adult injected animals resisted to the acute disease. most of the surviving mice, however, developed a chronic disease with a wasting syndrome and occurrence of paralysis. the chronic period of the disease was characterized by a persistent viral infection, si ... | 1975 | 168262 |
| macrophages genetically resistant to mouse hepatitis virus converted in vitro to susceptible macrophages. | genetic resistance to mouse hepatitis, which resides largely in the macrophages of resistant c3h mice, may be altered by exposing the cells in vitro to fluid from allogeneic mixed lymphocytes. a 1,000-fold increase in susceptibility was produced in these genetically resistant cells by exposure to this fluid. this presumed lymphokine was effective without producing any change in host adaption of the virus. | 1976 | 175127 |
| carrier state of antibody and viruses in a mouse breeding colony persistently infected with sendai and mouse hepatitis viruses. | in a large-scale mouse breeder colony persistently infected with sendai and mouse hepatitis viruses, most adult breeders 8 wk or more of age were shown to have antibodies to both viruses when monitored over a periof of 20 mo. antibody to sendai virus, apparently transmitted from the dam, was detected in 76% and 2% of mice aged 3 and 4 wk. respectively, and 64% and 100% of mice aged 6 and 8 wk, respectively. by seroconversion of sentinel cage-mates, a sendai virus-carrier state was demonstrated w ... | 1976 | 178959 |
| congenic strains of mice susceptible and resistant to mouse hepatitis virus. | a congenic strain of c3hss mice, which is histocompatible with c3h mice but differs from them in susceptibility to mouse hepatitis virus (mhv), has been developed by introducing the gene for susceptibility to the mhv-pri virus from the pri mice. this was accomplished by continual back-crossing of the hybrids to the c3h mice, but at the same time by selection of susceptibility by use of macrophage culture tests. after 20 back-crosses, a strain homozygous for susceptibility was produced by brother ... | 1976 | 135262 |
| response of nude mice to a mouse hepatitis virus isolated from a wasting nude mouse. | chronic active hepatitis was produced in nude mice after inoculation with a mouse hepatitis virus from a natural nude case which was incapable of causing fatal infection in haired heterozygous mice without cortisone. survival time of infected nude mice varied greatly from 1 to 10 weeks after the inoculation. degeneration and necrosis of hepatocytes, apparition of small and megalocytic hepatocytes, and infiltration of lymphocytes and plasma cells with fibrosis, were most remarkable in those which ... | 1976 | 180312 |
| an improved method for titration of mouse hepatitis virus type 3 in a mouse cell culture. | 1976 | 189731 | |
| age-dependent response of mice to a mouse hepatitis virus, mhv-s. | to a mouse hepatitis virus strain mhv-s, 4 week-old icr mice were shown to be fully resistant irrespective of route and dose of inoculation, and fatal infection was produced only with cortisone treatment. two-week-old mice also showed high resistance to mhv-s except for after intracerebral inoculation, and 60% of infected mice died. mice aged 1 week or less, however, died after intracerebral, intraperitoneal, subcutaneous and intranasal inoculation, while some of them survived after peroral inoc ... | 1977 | 194073 |
| naturally occurring mouse hepatitis virus infection in the nude mouse. | a group of athymic nude mice developed an unusual chronic wasting disease within 1-3 months after their arrival into the laboratory. affected nude mice had severe, acute-to-chronic, active hepatitis with multinucleated giant hepatocytes and fibrosis. vascular and central nervous system lesions were frequently present, giant cell peritonitis, ascites, and multinucleated giant cells in the intestinal epithelial villi were less frequently observed. mouse hepatitis virus was isolated from the livers ... | 1977 | 195136 |
| respiratory infection in mice with sialodacryoadenitis virus, a coronavirus of rats. | sialodacryoadenitis virus (sdav), a coronavirus of rats, evoked both serum neutralization and complement fixation antibody responses when inoculated intranasally in mice. weanling gnotobiotic cd-1 mice inoculated intranasally with 10(3.0) mean tissue culture infective doses of sdav remained asymptomatic. virus was recovered from the nasopharynx, trachea, and lung from day 2 to day 7. viral antigen was readily detected by indirect immunofluorescence in the lung but rarely in the nasopharynx. infe ... | 1977 | 201568 |
| serologic study on the prevalence of murine viruses in five canadian mouse colonies. | a serologic study was made of five canadian mouse colonies to determine the prevalence of antibodies to 11 murine viruses. a total of 139 sera from the five colonies were evaluated by complement fixation or hemagglutination inhibition methods. viral antibodies were present in all five colonies. antibodies to pneumonia virus of mice, minute virus of mice, theiler's encephalomyelitis virus, and reovirus type 3 were found in all five colonies. k-virus antibodies were present in four colonies. polyo ... | 1977 | 201796 |
| rna of mouse hepatitis virus. | the rna of mouse hepatitis virus, a coronavirus, was isolated from the virus released early in the infection and analyzed by sucrose gradient sedimentation and electrophoresis. it was found to consist of a piece of single-stranded rna of about 60s. its molecular weight was estimated to be 5.4 x 10(6) by electrophoresis in methylmercury-agarose gels. at least one third of the rna contained polyadenylated sequences. it is, therefore, probably positive stranded. the virus harvested late in the infe ... | 1978 | 207885 |
| virus infection in demyelinating diseases. | several animal and human demyelinating diseases of the central nervous system (cns) are associated with rna or dna viruses. these viruses infect cns cells lytically or persistently. they mainly belong to the group of envelope viruses which derive their envelope partly from the host cell membrane. the process of virus release may result in the appearance of new antigens of virus-infected cells or the incorporation of cell membrane material into the viral envelope. these changes may lead to an imm ... | 1978 | 211825 |
| in vitro growth characteristics and heterogeneity of mouse hepatitis virus type 3. | the in vitro virus yield of mhv3 reached 10(7) pfu/ml in mouse dbt cells infected with a virus suspension in hepes-buffered medium containing deae-dextran. the virus titer was 10(6) pfu/ml in the presence of 10 micrograms actinomycin d/ml mhv3 growth in dbt cells gave three peaks of density (1.10--1.14 g/cm3, 1.18--1.20 g/cm3, and 1.25--1.31 g/cm3) in sucrose gradients. all these peaks retained infectivity. | 1978 | 211989 |
| hepatosplenic myelosis in naturally occurring mouse hepatitis virus infection in the nude mouse. | 1978 | 214604 | |
| [effects of silver carbon on eliminating mouse hepatitis virus in water (author's transl)]. | 1978 | 215805 | |
| antigenic relationship of the feline infections peritonitis virus to coronaviruses of other species. | utilizing the direct and indirect fluorescent antibody procedure, the antigenic relationship of the feline infectious peritonitis virus (fipv) to 7 other human and animal coronaviruses was studied. fipv was found to be closely related to transmissible gastroenteritis virus (tgev) of swine. transmissible gastroenteritis virus and fipv were in turn antigenically related to human coronavirus 229e (hcv-229e) and canine coronavirus (ccv). an interesting finding in the study was that the 8 coronavirus ... | 1978 | 81044 |
| induction of lytic plaques by murine leukemia virus in murine sarcoma virus-transformed nonproducer mouse cells persistently infected with mouse hepatitis virus mhv-s. | kirsten murine sarcoma virus-transformed, nonproducer balb3t3 (k-balb) cells were persistently infected with mouse hepatitis virus, mhv-s. the cultures developed plaques after infection with murine leukemia viruses. if the murine leukemia virus-infected cultures were further submitted to the uv-xc assay, comparable numbers of xc plaques were obtained. the sensitivity to murine leukemia viruses, as determined by the uv-xc assay, was higher in mhv-s-infected cells as compared to uninfected k-balb ... | 1979 | 218897 |
| new strain of mouse hepatitis virus as the cause of lethal enteritis in infant mice. | a new strain of mouse hepatitis virus (mhv) was isolated from pooled gut suspensions from an epizootic of lethal enteritis in newborn mice. negative-contrast electron microscopy showed an abundance of coronavirus particles in the intestinal contents and intestinal epithelium of moribund mice. we found no other virus in the epizootic. dams seroconverted to mhv polyvalent antigen and to the agent isolated, but did not develop antibodies to other known mouse pathogens. virus propagated in nctc-1469 ... | 1979 | 222687 |
| pathology of diarrhea due to mouse hepatitis virus in the infant mouse. | two-day-old mice were inoculated orally with a mouse hepatitis virus which had been isolated from a suckling mouse with fatal diarrhea, and a systemic infection was shown to be established, though enterotropism of the isolate was evident. significant viral growth and syncytium forming lesions were first detectable in the intestines at day 2 postinoculation, followed by the development of focal necrotic hepatitis. the virus titer in both liver and intestine attained maximum levels at days 4 to 5. ... | 1979 | 224229 |
| pathogenesis of mouse hepatitis virus infection. the role of nasal epithelial cells as a primary target of low-virulence virus, mhv-s. | 1979 | 224286 | |
| giant cell formation in the brain of suckling mice infected with mouse hepatitis virus, jhm strain. | after intranasal inoculation with a neurotropic mouse hepatitis virus, jhm strain, suckling mice less than 2 weeks of age died in 4 or 5 days postinoculation, while those 3 weeks or more of age survived. in the brain lesions of mice 8 days or less of age, there appeared two different types of multinucleated giant cells. one of them which was irregular in shape with many virus particles, appeared mostly in the subependymal or perivascular areas after 72 h postinoculation and it seemed to have res ... | 1979 | 226760 |
| influence of the mouse hepatitis virus (mhv) infection on the growth of human tumors in the athymic mouse. | the growth characteristics and histological appearance of tumors resulting from transplantation of the tumor lines hep-2 and sw480 into pathogen-free and mouse hepatitis virus infected athymic mice were studied. subcutaneous or intraperitoneal implantation 1 x 10(6) neoplastic cells into pathogen-free animals resulted in tumor growth. subcutaneous transplants grew locally, surrounded by a capsule of connective tissue. the fibrovascular stroma supporting the neoplastic tissue was minimal and infi ... | 1979 | 437922 |
| prevalence of indigenous viruses in laboratory animal colonies in the united kingdom 1978-1979. | compared with the results of the previous serological survey of 1976-1977, it can be seen that mouse hepatitis virus is still prevalent in the mouse colonies and that corona viruses of rats are also common. the prevalence of sendai virus has increased considerably. however, the prevalence of reo 3 virus appeared to have decreased, although this may be the results of the different test used. | 1980 | 6257967 |
| glucan-induced modification of murine viral hepatitis. | glucan, a macrophage stimulant, was evaluated for its ability to alter survival and phagocytic dysfunction in mice challenged with mouse hepatitis virus strain mhv-a59. administration of glucan before the mice were challenged with the virus significantly prolonged median survival time but did not modify overall mortality compared with control mice given dextrose. maximal effectiveness was achieved when glucan was administered both before and after the viral challenge. in contrast to the marked h ... | 1980 | 7361108 |
| in vitro macrophage manifestation of cortisone-induced decrease in resistance to mouse hepatitis virus. | genetically resistant g3h mice routinely yielded macrophages that were resistant when grown in 90% horse serum. these mice also routinely yielded macrophages that were susceptible to the same virus, mhv (pri), in vitro after the mice had been treated with three intraperitoneal doses, of hydrocortisone. dexamethasone and prednisolone when similarly administered also increased the susceptibility of c3h macrophages taken from the treated animal, but progesterone and testosterone did not. in additio ... | 1981 | 6265581 |
| prostaglandins as probable mediators of the suppression of antibody production by mouse hepatitis virus infection. | 1981 | 6266331 | |
| inhibition of the mitotic response in regenerating mouse liver during viral hepatitis. | viral hepatitis caused by infection with murine hepatitis virus strain a59 has been shown to affect the mitotic response after partial hepatectomy in mice. normal mice showed a delayed response, whereas nude mice did not show any response, even 5 days after partial hepatectomy. | 1981 | 6268550 |
| ribonucleoprotein of avian infectious bronchitis virus. | the ribonucleoprotein (rnp) of avian infectious bronchitis virus (ibv) was examined by electron microscopy after shadowing with carbon/platinum. linear rnp strands up to 6.7 microns in length, from three ivb strains, were sensitive to both pancreatic rnase and to proteases. these strands were obtained from spontaneously disrupted complete particles but not from disrupted incomplete particles that lacked rnp. they were also released from nonidet p40-disrupted particles and could be isolated on su ... | 1981 | 6268741 |
| early interaction between mouse hepatitis virus 3 and cells. | the interaction between mouse hepatitis virus 3 (mhv3) and cells was studied in order to investigate whether or not early events occurring after infection could be involved in the difference in virus replication seen between mouse strains with different genetic sensitivities to mhv3 infection. kinetic data showed that mhv3 uptake by both macrophages and l cells was time- and temperature-dependent. in addition, treatment of cells with cytochalasin b or prostaglandin e1, prior to virus infection, ... | 1981 | 6275022 |
| viral protein synthesis in mouse hepatitis virus strain a59-infected cells: effect of tunicamycin. | we identified eight protein species in virions of mouse hepatitis virus strain a59. based on their sizes, prosthetic groups, and locations in virions, these proteins were designated gp180/e2, gp90/e2, pp54/n, gp26.5/e1, gp25.5/e1, p24/e1, p22/x, and p14.5/y. the positions of the last two proteins in virions are not known. host protein synthesis in sac(-) cells infected with mouse hepatitis virus strain a59 was inhibited, and the following novel proteins appeared: gp150, gp90, p54, gp26.5, gp25.5 ... | 1981 | 6275093 |
| the pathogenesis of chronic viral hepatitis in the nude mouse and its influence on liver regeneration after partial hepatectomy. | the pathogenesis of chronic viral hepatitis due to infection with mouse hepatitis virus type 1 has been followed over a period of 30 days in the nu/nu mouse. the initial histopathology is that of a focal hepatic necrosis which evolves into a chronic hepatitis with cirrhosis. the histopathology of the experimentally induced hepatitis with mouse hepatitis type 1 is indistinguishable from that produced by the disease in nude mouse colonies due to the natural infection in the united kingdom and also ... | 1981 | 6275875 |
| assembly of mouse hepatitis virus strain jhm. | 1981 | 6278874 | |
| messenger rnas of mouse hepatitis virus a59: isolation and characterization, translation in xenopus laevis oocytes of rnas 3, 6 and 7, uv target sizes of the transcription templates. | 1981 | 6278883 | |
| genome structure of mouse hepatitis virus: comparative analysis by oligonucleotide mapping. | several natural variants of mouse hepatitis viruses have been compared by the t1-oligonucleotide fingerprinting technique. in general, they have diverged quite extensively. however, mhv-3, a hepatotropic strain, and a59, a nonpathogenic strain, were found to be extremely related. yet, each of them contains 2-4 specific oligonucleotides. one of the mhv-3-specific oligonucleotides was mapped in 30s poly(a)-containing rna or 6-7 kb from the 3-end and the other near the 5'-end of the genome. these t ... | 1981 | 6278897 |
| clinical, pathologic, and serologic features of an epizootic of mousepox in minnesota. | an epizootic of mousepox in two colonies of mice was described. clinical signs, morbidity and mortality rates, and necropsy lesions were substantially influenced by husbandry practices, intercurrent diseases (sendai pneumonia, mouse hepatitis virus), and total body x-irradiation. in one colony, 54 mice were necropsied; 19 mice had cutaneous or visceral lesions of mousepox although none had combined visceral and cutaneous lesions. in the other colony, 20 mice were necropsied, and 10 mice had cuta ... | 1981 | 6281560 |
| role of macrophages and interferon in natural resistance to mouse hepatitis virus infection. | 1981 | 6171386 | |
| heterologous response of antiserum-treated cell clones from a persistently infected dbt cell line to mouse hepatitis virus. | from dbt cells persistently infected with mouse hepatitis virus jhm strain (jhm-cc), a cell line producing neither infectious virus nor intracellular viral antigen was obtained after two passages in the presence of antiserum. in addition, 11 cell clones were manipulated from jhm-cc and found to be also free from the virus. these newly obtained cell line and 4 of 11 cell clones were shown to be resistant to jhm and the virus recovered from jhm-cc (jhm-ccv), while the other 7 cell clones were susc ... | 1982 | 6302351 |
| mouse hepatitis virus immunofluorescence in formalin- or bouin's-fixed tissues using trypsin digestion. | mouse hepatitis viral antigens were demonstrated by immunofluorescence in formalin- and bouin's-fixed tissues processed routinely for histopathology followed by partial digestion with trypsin. staining was superior in tissues fixed in formalin and was not diminished in tissue sections from paraffin blocks stored at room temperature as long as 2 years. the relative ease of this procedure and the commercial availability of reagents makes this a useful technique for the definitive diagnosis of mous ... | 1982 | 6281569 |
| sequence relationships between the genome and the intracellular rna species 1, 3, 6, and 7 of mouse hepatitis virus strain a59. | we have shown by t(1) oligonucleotide fingerprinting that the genome of mouse hepatitis virus strain a59 and its intracellular rna 1 have identical fingerprints and that rna 1 and the subgenomic rnas 3, 6, and 7 contain common sequences. to localize the homologous region between the rnas, we compared fingerprints of the 3' terminus of the genome with those of rna 7. the genome was partially degraded with alkali, and polyadenylate-containing fragments were purified by oligodeoxythymidylate-cellul ... | 1982 | 6283166 |
| effects of experimental infection of the deer mouse (peromyscus maniculatus) with mouse hepatitis virus. | three deer mice (peromyscus maniculatus) given 10(4) tcid50 of mouse hepatitis virus -s intranasally all had significant serum neutralization titers to mouse hepatitis virus -s 3 weeks later. ten other deer mice were given 10(3) tcid50 of mouse hepatitis virus -3 intranasally and placed with sentinel laboratory mice (cf1, c3h/rv, and nu/nu) and uninoculated deer mice. one inoculated deer mouse died, but mouse hepatitis virus was not isolated. no other inoculated or uninoculated animals exhibited ... | 1982 | 6285078 |
| replication of mouse hepatitis virus: negative-stranded rna and replicative form rna are of genome length. | there are seven virus-specific mrna species in mouse hepatitis virus-infected cells (lai et al., j. virol. 39:823-834, 1981). in this study, we examined virus-specific negative-stranded rna to determine whether there are corresponding multiple negative-stranded rnas. intracellular rna from mouse hepatitis virus-infected cells was separated by agarose gel electrophoresis, transferred to nitrocellulose membranes, and hybridized to positive-stranded genomic 60s [32p]rna. only a single rna species o ... | 1982 | 6292513 |
| murine coronaviruses: isolation and characterization of two plaque morphology variants of the jhm neurotropic strain. | two plaque-size variants of the neurotropic jhm strain of mouse hepatitis virus have been isolated from the virus stock after eight serial passages in suckling mouse brain. one variant, jhm-dl, produces large plaques, while the other, jhm-ds, produces small plaques in tissue culture. ds replicates more slowly, has a lower virus yield in vitro, and is less virulent for mice than dl. they also differ in their pathogenicity for mice: jhm-dl infection results in acute encephalomyelitis while jhm-ds ... | 1982 | 6296277 |
| isolation of a coronavirus during studies on puffinosis, a disease of the manx shearwater (puffinus puffinus). | a virus was isolated from 2 day-old mice inoculated with homogenates of either the lungs or blood of 2 different shearwaters affected by puffinosis. examination of infected suckling mouse brain and infected nctc-1469 (mouse liver) cell cultures, by electron microscopy, revealed virus particles and inclusion bodies characteristic of a coronavirus. neutralization, complement fixation and fluorescent antibody tests showed that the virus was related to mouse hepatitis virus. the virus was not isolat ... | 1982 | 7125912 |
| [the activity of serine dehydratase enzyme of liver tissue in experimental murine infection with mouse hepatitis virus 3]. | 1983 | 9704124 | |
| temperature-sensitive mutants of mouse hepatitis virus strain a59: isolation, characterization and neuropathogenic properties. | twenty 5-fluorouracil-induced temperature-sensitive (ts) mutants of mouse hepatitis virus strain a59 were isolated from 1284 virus clones. mutants were preselected on the basis of their inability to induce syncytia in infected cells at the restrictive temperature (40 degrees) vs the permissive temperature (31 degrees). of these mutants, only those with a relative plating efficiency 40 degrees/31 degrees of 3 x 10(-3) or smaller were kept. virus yields at 40 degrees compared to 37 degrees and 31 ... | 1983 | 6301146 |
| fusion resistance and decreased infectability as major host cell determinants of coronavirus persistence. | mouse hepatitis virus persists in cultures of a subline (designated lm-k) of mouse lm cells but produces a lytic infection in l-2 cells. persistence in the lm-k cells was not accompanied by production of ts mutants or of soluble anti-mhv factors. infectious center assay demonstrated an approximately 500-fold lower level of infectibility by mhv of the lm-k cells as compared to l-2 cells. on an infected cell basis, production levels of infectious progeny and viral rna were comparable between the t ... | 1983 | 6310865 |
| antigenic characterization of tettnang virus: complications caused by passage of the virus in mice from a colony enzootically infected with mouse hepatitis virus. | neutralization assays were undertaken for the purpose of antigenically characterizing three strains of tettnang virus from two geographic regions. the previously reported relationship of tettnang virus strains to mouse hepatitis virus (mhv) was confirmed. however, the precise relationship of the tettnang strains to prototype mhv strains was obscured in our study by the finding that the isolates had been passaged in mice from a colony subclinically infected with mhv. an egyptian strain of tettnan ... | 1983 | 6312821 |
| the effect of mouse hepatitis virus infection on the microcirculation of the liver. | mouse hepatitis virus type 3 infection results in strain-dependent liver disease. the effects of mouse hepatitis virus type 3 on the microcirculation of the liver in both fully susceptible (balb/cj) and fully resistant (a/j) mice were studied. in balb/cj mice, 6 to 12 hr following infection, abnormalities in liver blood flow were observed which consisted of granular blood flow in both terminal hepatic and terminal portal venules. in addition, sinusoidal microthrombi were present predominantly in ... | 1983 | 6313508 |
| histopathological characterization of the naturally occurring hepatotropic virus infections of nude mice. | in the last ten years the mutant athymic nude mouse has been used by many researchers as a model for studying pathological conditions in an immunodeficient host. a major problem with such mice is their susceptibility to viral infections indigenous to murine stocks, such as the hepatotropic mouse coronaviruses. in an effort to define the hepatotropic virus diseases indigenous to nude mice this paper details the pathogenesis and associated comparative histopathology of three common strains of mous ... | 1984 | 6321712 |
| assembly in vitro of a spanning membrane protein of the endoplasmic reticulum: the e1 glycoprotein of coronavirus mouse hepatitis virus a59. | the e1 glycoprotein of coronavirus mouse hepatitis virus a59 was synthesized in vitro by translation of viral mrna in the presence of dog pancreatic microsomes. its disposition in the membrane was investigated by digestion with proteases and by selective nh2-terminal labeling. the protein spans the membrane, but only small portions from the nh2 and cooh terminus are exposed respectively in the lumenal and cytoplasmic domains; the bulk of the molecule is apparently buried in the membrane. the pro ... | 1984 | 6324191 |
| the carbohydrates of mouse hepatitis virus (mhv) a59: structures of the o-glycosidically linked oligosaccharides of glycoprotein e1. | two size classes of o-glycosidically linked oligosaccharides were liberated from glycoprotein e1 of mouse hepatitis virus (mhv) a59 by reductive beta-elimination and separated by h.p.l.c. the structures of the reduced oligosaccharides were determined by successive exoglycosidase digestions and by methylation analyses involving combined capillary gas chromatography-mass spectrometry and mass fragmentography after chemical ionization with ammonia. oligosaccharide a (neu5ac alpha 2----3 gal beta 1- ... | 1984 | 6325180 |
| ten years-long survey on pathogen status of mouse and rat breeding colonies. | eleven pathogens including p. aeruginosa, salmonella spp., e. coli o115a, c: k(b), p. pneumotropica, b. bronchiseptica, c. kutscheri, tyzzer's organism, m. pulmonis, sendai virus, mhv and syphacia spp. were surveyed in 217 mouse and rat breeding colonies during 1972-1981. in conventional animals, p. pneumotropica and/or syphacia spp. were detected in nearly 90% of 89 mouse and 64 rat colonies. sendai virus, m. pulmonis, p. aeruginosa and mhv were positive in 51.7 to 23.6% of the colonies, and ty ... | 1984 | 6088258 |
| delayed-type hypersensitivity in mouse hepatitis virus infection in mice. | delayed-type hypersensitivity in mouse hepatitis virus infection was studied with intraperitoneally infected mice. twenty-four hours after eliciting injection with virus-infected cell lysate on day 8 postinfection, specific footpad swelling response was observed with infiltration of mononuclear cells. the response was not enhanced by cyclophosphamide treatment made 2 days before infection. the adoptive transfer of the response from infected mice to h-2 compatible recipients was successful with s ... | 1984 | 6088859 |
| analysis of genomic and intracellular viral rnas of small plaque mutants of mouse hepatitis virus, jhm strain. | the genomic rna and intracellular rna of mouse hepatitis virus, strain jhm (mhv-jhm) and two plaque mutants (1a and 2c), which have been isolated from a persistently infected culture (jhm-cc), have been analyzed by t1-resistant oligonucleotide finger-printing. the genomic rna of the virus population (jhm-cc virus) released from different passage levels of the same persistent infection has also been analyzed. the analysis shows the locations within the genomic and intracellular rnas of more than ... | 1984 | 6093379 |
| in vivo and in vitro models of demyelinating disease x. a schwannoma-l-2 somatic cell hybrid persistently yielding high titres of mouse hepatitis virus strain jhm. | following infection of rn2 rat schwannoma cells with unfiltered jhmv inocula, a cell line with an altered phenotype evolved, which was shown to be a somatic cell hybrid of rn2 and mouse l-2 cells. this cell line, ej, persistently yields jhmv at titres greater than 10(6) pfu/ml and does not show the suppression of virus production at 39.5 degrees c that is characteristic of a persistently infected rn2 line. intracellular viral nucleocapsids are demonstrated. cloning of ej hybrids yields cell line ... | 1984 | 6099665 |
| host genetic control of mouse hepatitis virus type-4 (jhm strain) replication. ii. the gene locus for susceptibility is linked to the svp-2 locus on mouse chromosome 7. | peritoneal macrophages were used to analyze host genetic control of mhv-4 infection in the 14 recombinant-inbred strains between susceptible swr/j and resistant sjl/j progenitor strains. 4 strains were resistant to mhv-4 infection, while the remaining 10 strains were productively infected. based upon the strain distribution pattern of susceptibility the gene locus for mhv-4 susceptibility is linked to the svp-2 locus on the proximal end of mouse chromosome 7. this strain distribution pattern of ... | 1984 | 6101098 |
| pathogenicity of mouse hepatitis virus for preimplantation mouse embryos. | mouse embryos which were hatched from the zona pellucida in vitro in the presence of mouse hepatitis virus (mhv) or outgrown on coverslips and then exposed to mhv were shown by immunohistochemical staining to have virally infected trophoblast cells. zona-intact embryos incubated with mhv for 48 h (2-cell embryos) or 1.5 h (blastocysts) were resistant to infection. morulae and early blastocysts collected from donor mice experimentally infected with mhv were not infected, but the medium in which t ... | 1985 | 2982018 |
| signal recognition particle-dependent insertion of coronavirus e1, an intracellular membrane glycoprotein. | the membrane insertion of the e1 protein of a coronavirus, mouse hepatitis virus a59, was studied in a wheat germ cell-free translation system. e1 is a transmembrane protein spanning the lipid bilayer several times. it is synthesized without a cleavable signal sequence, localized intracellularly, and not transported to the cell surface. it thus represents a model intracellular protein. we found that the synthesis of e1 is specifically and stably blocked by the addition of signal recognition part ... | 1985 | 2985561 |
| difference in sensitivity to interferon among mouse hepatitis viruses with high and low virulence for mice. | mouse hepatitis viruses (mhv) of different virulence for mice were studied with respect to interferon (ifn) sensitivity. the growth of low-virulent mhv-s and intermediately virulent mhv-jhm was significantly suppressed in ifn-treated l cells compared with untreated cells. however, a comparable suppression of the growth of highly virulent mhv-2 was not observed in ifn-treated cells. this differential effect of ifn treatment could also be demonstrated at the level of viral mrna and viral proteins. ... | 1985 | 2998070 |
| virus-induced central positional nystagmus in mice. | geotropic direction-changing nystagmus in lateral body positions was observed in 4-week-old balb/c mice after intracerebral injection with a temperature-sensitive mutant of mouse hepatitis virus. the positional nystagmus was detected already 2 days after infection and it lasted half a year at least. the nystagmic responses of the semicircular canals were also evaluated before and after infection. they were unaltered during the disease, which was clinically manifested by general weakness, ataxia ... | 1985 | 2998148 |
| proteolytic cleavage of the e2 glycoprotein of murine coronavirus: host-dependent differences in proteolytic cleavage and cell fusion. | cell fusion induced by infection with mouse hepatitis virus strain a59 (mhv-a59) varied markedly in extent and time course in four different murine cell lines. when inoculated at a multiplicity of 3 to 5 pfu per cell, the sac-, l2, and dbt cell lines began to fuse by 7 h, were fused into confluent syncytia by 9 to 12 h, and peeled from the substrate by 10 to 14 h. these virulent virus-cell interactions were in striking contrast to the moderate interaction of mhv-a59 with the 17 cl 1 cell line, i ... | 1985 | 2999444 |
| effect of exogenous mouse interferon on murine fulminant hepatitis induced by mouse hepatitis virus type 2. | we investigated the effect of exogenous mouse alpha- + beta-interferon produced by mouse l cells on the growth of mouse hepatitis virus type 2 (mhv-2) in the liver, the development of liver cell necrosis, and survival in murine fulminant hepatitis induced by mhv-2. murine fulminant hepatitis was induced in 4-week-old male icr mice by intraperitoneal inoculation of mhv-2. mouse interferon (10(3) iu/mouse/day) was intraperitoneally injected every day. exogenous mouse interferon suppressed both the ... | 1986 | 3002736 |
| translation and processing of mouse hepatitis virus virion rna in a cell-free system. | the first event after infection with mouse hepatitis virus strain a59 (mhv-a59) is presumed to be the synthesis of an rna-dependent rna polymerase from the input genomic rna. the synthesis and processing of this putative polymerase protein was studied in a cell-free translation system utilizing 60s rna from mhv-a59 virions. the polypeptide products of this reaction included two major species of 220 and 28 kilodaltons. kinetics experiments indicated that both p220 and p28 appeared after 60 min of ... | 1986 | 3018279 |
| [a comparison of two antigen strains of each of mouse hepatitis virus and mouse adenovirus for detection of complement fixation antibody in mouse and rat sera]. | detection rates of complement fixation antibodies in mice and rats were compared between two antigen strains of each of mouse hepatitis virus (mhv) and mouse adenovirus (mav). among 66 and 47 naturally infected mhv-positive sera of mice (18 facilities) and rats (16 facilities) respectively, 17 mouse and 21 rat sera reacted with both nu-67 and mhv-2 strains, but 49 mouse and 25 rat sera were positive to nu-67 strain alone. only one rat serum reacted with mhv-2 strain alone. in comparison with k87 ... | 1986 | 3026815 |
| identification of two epitopes in the carboxyterminal 15 amino acids of the e1 glycoprotein of mouse hepatitis virus a59 by using hybrid proteins. | cdna fragments coding for the carboxy terminus of the e1 envelope glycoprotein from mouse hepatitis virus a59, a coronavirus, were cloned into the bacterial expression vector pex. clones expressing e1 antigenic determinants were selected with a polyclonal anti-e1 antibody and used for immunization of rabbits and for affinity purification of existing polyclonal antisera. immunofluorescence testing and immunoperoxidase labeling of coronavirus-infected cells showed that these reagents were monospec ... | 1986 | 2431163 |
| an immunological analysis of natural resistance to mouse hepatitis virus (jhmv strain) infection in c3h mice. | 1. since the development of resistance against mouse hepatitis virus (jhmv strain) coincides with the maturation of the immune system, we studied the possible role of distinct immunological components in the resistance of adult mice during jhmv infection. 2. adult c3h mice naturally resistant to jhmv were rendered susceptible to infection by lethal 60co-irradiation and were subsequently reconstituted with limiting numbers of syngeneic bone marrow cells or spleen cells. 3. resistance or susceptib ... | 1987 | 2825892 |
| characterization of mouse hepatitis virus-reactive t cell clones. | 1987 | 2829551 | |
| neuropathogenicity of mutant strains of mouse hepatitis virus, 1a and 2c, from dbt cells persistently infected with jhm strain. | 1987 | 2829556 | |
| delayed increase in plasma lactic dehydrogenase activity in mouse hepatitis virus-infected mice subsequently infected with lactic dehydrogenase virus. | 1987 | 2830435 | |
| accumulation and chemotaxis of natural killer/large granular lymphocytes at sites of virus replication. | a model for monitoring the accumulation of natural killer cell/large granular lymphocytes (nk/lgl) at a site of virus replication was studied by using mice infected i.p. with either lymphocytic choriomeningitis virus (lcmv), murine cytomegalovirus (mcmv), mouse hepatitis virus (mhv), pichinde virus, or vaccinia virus. an i.p. but not i.v. infection resulted in a localized increase in nk/lgl cell number (a fourfold to greater than 20-fold increase) and augmentation (a 10- to 20-fold increase) of ... | 1987 | 3027167 |
| detection of a murine coronavirus nonstructural protein encoded in a downstream open reading frame. | mouse hepatitis virus (mhv) gene 5 contains two open reading frames. we have expressed the second open reading frame of this gene (gene 5 orf 2) in an escherichia coli expression system. this system utilized a plasmid which contained the promoter and the first 36 codons of the reca gene fused in frame with the mhv gene 5 orf 2, which is fused in turn to the beta-galactosidase gene. the protein product of this gene fusion was used to raise antibody to gene 5 orf 2. the specificity of the antibody ... | 1988 | 2834866 |
| synthesis of virus-specific rna in permeabilized murine coronavirus-infected cells. | we have developed a permeabilized cell system for assaying mouse hepatitis virus-specific rna polymerase activity. this activity was characterized as to its requirements for mono- and divalent cations, requirements for an exogenous energy source, and ph optimum. this system faithfully reflects mhv-specific rna synthesis in the intact cell, with regard to both its time of appearance during the course of infection and the products synthesized. the system is efficient and the rna products were iden ... | 1988 | 2842958 |
| reactivities of 4 murine coronavirus antigens with immunized or naturally infected rat sera by enzyme linked immunosorbent assay. | four murine coronavirus antigens, sialodacryoadenitis virus (sdav) strain tg, parker's rat coronavirus (pcv) strain 8190, mouse hepatitis virus (mhv) strains s and nuu, were examined for their reactivities to hyperimmunized and naturally infected rat sera by elisa. with the immunized sera, sdav and pcv antigens reacted best with respective homologous sera. mhv antigens reacted with all antisera, anti-sdav, anti-pcv, and anti-mhv-s at approximately the same level, and mhv-s showed a slightly high ... | 1988 | 2843391 |
| membrane integration and intracellular transport of the coronavirus glycoprotein e1, a class iii membrane glycoprotein. | the e1-glycoprotein (mr = 26,014; 228 amino acids) of mouse hepatitis virus a59 is a class iii membrane glycoprotein which has been used in this study as a model system in the study of membrane integration and protein transport. the protein lacks an nh2-terminal cleavable signal sequence and spans the viral membrane three times. hydrophobic domains i and iii could serve as signal sequences for cotranslational membrane integration. domain i alone was sufficient to translocate the hydrophilic nh2 ... | 1988 | 2844793 |
| virus strain specificity of challenge immunity to coronavirus. | the resistance of immunized mice to challenge with the same or a different strain of mouse hepatitis virus (mhv) was examined as a model of challenge immunity to coronavirus infection. genetically susceptible balb/cbyj mice were given an intranasal immunizing infection of respiratory-type mhv-jhm, mhv-s, or enterotropic mhv-y. control mice were sham-immunized with sterile tissue culture fluid. recovered mice were challenged intranasally with mhv-jhm, mhv-s or sterile tissue culture fluid at 30 d ... | 1989 | 2468325 |
| monoclonal antibodies to the matrix (e1) glycoprotein of mouse hepatitis virus protect mice from encephalitis. | monoclonal antibodies to the matrix or e1 glycoprotein of mouse hepatitis virus (mhv) were tested for their ability to protect mice from a normally lethal challenge of mhv-4. four antibodies were tested, and two of these, j.1.3 and j.3.9, were protective. protection did not correlate with virus neutralization in vitro, antibody isotype, recognition of a unique e1 antigenic site, or dependence on complement in vivo. survival from acute encephalitis was followed by subacute demyelination, as has b ... | 1989 | 2535900 |