| serosurvey of pathogens in domestic dogs on the border of noël kempff mercado national park, bolivia. | the threat of disease transmission from domestic animals to wildlife has become recognized as an increasing concern within the wildlife community in recent years. domestic dogs pose a significant risk as reservoirs for infectious diseases, especially for wild canids. as part of a multifaceted ecologic study of maned wolves and other canids in the large, remote noël kempff mercado national park (nkmnp) in northeastern bolivia, 40 domestic dogs in two villages and at two smaller settlements border ... | 2008 | 18432094 |
| molecular detection of tick-borne protozoal and ehrlichial infections in domestic dogs in south africa. | a total of 1,138 blood specimens were collected over a 6-year period (2000-2006) from domestic dogs in south africa. specimens from domestic dogs were obtained from the onderstepoort veterinary academic hospital (ovah) in pretoria, the society for the prevention of cruelty to animals (johannesburg, durban, east london and bloemfontein) and private practices from four provinces (gauteng, mpumalanga, kwazulu-natal and western cape). all specimens were screened for babesia, theileria, hepatozoon an ... | 2008 | 18502588 |
| a study of cross-reactivity in serum samples from dogs positive for leishmania sp., babesia canis and ehrlichia canis in enzyme-linked immunosorbent assay and indirect fluorescent antibody test. | to verify the presence of cross-reaction among leishmaniosis, ehrlichiosis and babesiosis in serological diagnostics used in human visceral leishmaniasis control programs, serum samples from leishmaniasis endemic and non-endemic areas were collected and tested by indirect fluorescent antibody (ifat) and enzyme-linked immunosorbent assay (elisa). all serum samples from endemic areas were positive for leishmania sp., by elisa and ifat, 51% positive for babesia canis and 43% for ehrlichia canis by ... | 2008 | 18554433 |
| factors associated with the occurrence of epistaxis in natural canine leishmaniasis (leishmania infantum). | canine leishmaniasis (canl) is a common cause of epistaxis in dogs residing in endemic areas. the pathogenesis of canl-associated epistaxis has not been fully explored because of the limited number of cases reported so far. | 2008 | 18564224 |
| detection of a theileria species in dogs in south africa. | a theileria species was detected by pcr in blood samples collected from dogs in the pietermaritzburg area and was also found in dogs presented at the outpatients clinic of the onderstepoort veterinary academic hospital (ovah), in the pretoria area, south africa. in the pietermaritzburg area, 79 of the 192 samples were positive, while 3 out of 1137 of the onderstepoort samples were positive. three positive samples from pietermaritzburg were co-infected with ehrlichia canis. pcr positive samples w ... | 2008 | 18687528 |
| cultivation and molecular identification of ehrlichia canis and ehrlichia chaffeensis from a naturally co-infected dog in venezuela. | diagnosis of canine ehrlichiosis in venezuela is normally performed by examination of buffy coat smears (bcs). characteristic inclusion bodies are frequently observed in leukocytes and platelets from dogs with clinical signs of the disease. | 2008 | 18761516 |
| serological survey of ehrlichia canis in stray dogs from yucatan, mexico, using two different diagnostic tests. | canine ehrlichiosis is a disease produced by the rickettsial organism ehrlichia canis. reported prevalence may vary greatly depending on the test and sampling method used. for the serological detection of antibodies against e. canis, the indirect immunofluorescence antibody test (ifa) is considered the gold standard. however, other available serological techniques such as the indirect immunoperoxidase technique (ipt) have not yet been tested and may be efficient in detecting specific antibodies. ... | 2009 | 18945189 |
| retrospective clinical and molecular analysis of conditioned laboratory dogs (canis familiaris) with serologic reactions to ehrlichia canis, borrelia burgdorferi, and rickettsia rickettsii. | dogs are susceptible to different tickborne infections, including members of the anaplasmataceae (ehrlichia canis, e. ewingii, e. chaffeensis, anaplasma phagocytophilum, a. platys), borrelia burgdorferi, and rickettsia rickettsii. these diseases can manifest with clinical signs including fever, anorexia, malaise, lameness, rash, and bleeding episodes; however, these signs are nonpathognomonic, and infections can occur in the absence of clinical signs. hematologic abnormalities can include leukop ... | 2008 | 18947166 |
| canine inflammatory myopathy associated with leishmania infantum infection. | inflammatory myopathy associated with several infectious diseases occurs in dogs including those caused by toxoplasma gondii, neospora caninum, ehrlichia canis and hepatozoon canis. however, muscle disease due to leishmania infection has been poorly documented. the aim of this study was to examine the distribution and types of cellular infiltrates and expression of mhc class i and ii in muscle biopsies obtained from 15 male beagle dogs from a breeder group with an established diagnosis of leishm ... | 2009 | 19084398 |
| longitudinal quantification of ehrlichia canis in experimental infection with comparison to natural infection. | ehrlichia canis is a major tick-borne bacterial pathogen of dogs. quantitative real-time pcr was evaluated for the detection of e. canis in naturally (ni) and experimentally infected (ei) dogs. dna was extracted from blood, spleen and conjunctival swabs of experimentally infected dogs pre- and post-infection (pi), and during doxycycline therapy, and from blood and conjunctivas of naturally infected dogs. the primers and probe were designed to amplify a 93bp fragment of the single copy e. canis 1 ... | 2009 | 19128893 |
| toward diagnosing leishmania infantum infection in asymptomatic dogs in an area where leishmaniasis is endemic. | the most frequently used diagnostic methods were compared in a longitudinal survey with leishmania infantum-infected asymptomatic dogs from an area of italy where leishmaniasis is endemic. in february and march 2005, 845 asymptomatic dogs were tested by an immunofluorescence antibody test (ifat), a dipstick assay (ds), and an enzyme-linked immunosorbent assay (elisa) for l. infantum and by ifat for ehrlichia canis. dogs seronegative for l. infantum were further parasitologically evaluated by mic ... | 2009 | 19129471 |
| evaluation of a serum-based pcr assay for the diagnosis of canine monocytic ehrlichiosis. | the aim of this study was to estimate the relative diagnostic sensitivity and specificity of a polymerase chain reaction (pcr) assay in the serum of dogs with naturally occurring non-myelosuppressive canine monocytic ehrlichiosis (cme), and to investigate the association between pcr positivity and immunofluorescence antibody (ifa) titres for ehrlichia canis. serum samples obtained from 38 dogs with non-myelosuppressive cme and 12 healthy dogs were analyzed retrospectively. each serum sample was ... | 2009 | 19419823 |
| major species-specific antibody epitopes of the ehrlichia chaffeensis p120 and e. canis p140 orthologs in surface-exposed tandem repeat regions. | ehrlichia chaffeensis and e. canis have a small subset of tandem repeat (tr)-containing protein orthologs, including p120/p140, which elicit strong antibody responses. the tr regions of these protein orthologs are immunoreactive, but the molecular characteristics of the p120/p140 epitopes have not been determined. in this study, the immunodeterminants of the e. chaffeensis p120 and e. canis p140 were identified and molecularly defined. major antibody epitope-containing regions of both p120 and p ... | 2009 | 19420187 |
| rapid screening and cultivation of ehrlichia canis from refrigerated carrier blood. | | 2009 | 19438630 |
| an acute severe ehrlichiosis in a dog experimentally infected with a new virulent strain of ehrlichia canis. | | 2009 | 19548986 |
| occurrence of dirofilaria immitis and tick-borne infections caused by anaplasma phagocytophilum, borrelia burgdorferi sensu lato and ehrlichia canis in domestic dogs in france: results of a countrywide serologic survey. | the occurrence of dirofilaria immitis antigen and antibodies against tick-borne pathogens in french dogs has been analysed based on 1,050 blood samples. serum samples of 919 dogs (group a) were sent for a variety of diagnostic investigations, further 131 dogs (group b) were tested for a tentative diagnosis of heartworm disease. all samples were tested for d. immitis antigen. samples in group a were also tested for specific antibodies against three tick-borne pathogens (anaplasma phagocytophilum, ... | 2009 | 19575231 |
| comparison of two clinic-based immunoassays with the immunofluorescence antibody test for the field diagnosis of canine monocytic ehrlichiosis. | the aim of this study was to compare a clinic-based enzyme-linked immunosorbent assay (immunocomb-eliza, biogal, israel) and a field-based dot-blot enzyme-linked immunoassay (dbelia) with the indirect immunofluoresence antibody test (ifat) used for the field diagnostics of canine monocytic ehrlichiosis (cme). the total test samples was 60; out of them 52 serum samples came from naturally exposed police german shepherd dogs and 8 from adult mongrels infected with viable ehrlichia canis propagated ... | 2009 | 19621766 |
| babesia canis and other tick-borne infections in dogs in central poland. | vector-borne infections constitute increasing health problem in dogs worldwide, including sled dogs, dramatically decreasing the fitness of working dogs and even leading to death. in the period 2006-2008 eighty-two blood samples were collected from eight sled dog kennels in central poland. the prevalence of four vector-borne infections (babesia canis, bartonella sp., anaplasma/ehrlichia and borrelia burgdorferi) was estimated in 82 sled dogs using pcr and nested pcr for diagnosis and the same me ... | 2009 | 19837515 |
| absence of myelofibrosis in dogs with myelosuppression induced by ehrlichia canis infection. | bone marrow (bm) pathology was assessed in 10 dogs with ehrlichia canis-induced aplastic pancytopenia. bm core biopsy sections were stained with haematoxylin and eosin and with haematoxylin/van gieson and gordon and sweets' reticulin stain for the detection of collagen and reticulin fibres, respectively. iron stores were assessed by perls' prussian blue staining. there was no significant deposition of collagen or reticulin in any sample, but in seven dogs the bm was depleted of haemosiderin. the ... | 2010 | 19906384 |
| detection of babesia canis subspecies and other arthropod-borne diseases in dogs from tirana, albania. | summary. the importance of arthropod-borne diseases increased in the recent past in particular due to frequent travel with dogs in or by importing of dogs from regions with endemic occurrence of these diseases. while the epidemiological situation is well known for the western parts of the mediterranean, only limited data is available for eastern europe and the balkans. thirty clinically healthy dogs from suburban areas of tirana, albania, were tested for babesia canis, hepatozoon spp., leishmani ... | 2009 | 19915816 |
| canine babesiosis in northern portugal and molecular characterization of vector-borne co-infections. | abstract: | 2010 | 20377861 |
| comparison of selected canine vector-borne diseases between urban animal shelter and rural hunting dogs in korea. | abstract: a serological survey for dirofilaria immitis, anaplasma phagocytophilum, ehrlichia canis, and borrelia burgdorferi infections in rural hunting and urban shelter dogs mainly from southwestern regions of the republic of korea (south korea) was conducted. from a total of 229 wild boar or pheasant hunting dogs, the number of serologically positive dogs for any of the four pathogens was 93 (40.6%). the highest prevalence observed was d. immitis (22.3%), followed by a. phagocytophilum (18.8% ... | 2010 | 20377869 |
| imported and travelling dogs as carriers of canine vector-borne pathogens in germany. | abstract: | 2010 | 20377872 |
| severe hepatitis associated with acute ehrlichia canis infection in a dog. | | 2010 | 20384948 |
| serological study of selected vector-borne diseases in shelter dogs in central spain using point-of-care assays. | we evaluated the prevalence of selected vector-borne diseases in 131 dogs in an animal shelter in central spain using point-of-care assays (snap 4dx and snap leishmania; idexx laboratories, westbrook, me). the snap 4dx detects dirofilaria immitis (di) antigen and antibodies against ehrlichia canis (ec), borrelia burgdorferi (bb), and anaplasma phagocytophylum (aph); the snap leishmania kit detects antibodies against leishmania infantum (li). dogs were classified as healthy or sick based on physi ... | 2010 | 20420531 |
| prevalence of anti-platelet antibodies in dogs naturally co-infected by leishmania infantum and ehrlichia canis. | thirty naturally infected dogs were divided into three groups (10 animals each): group i (canine leishmaniosis), group ii (canine ehrlichiosis) and group iii (dogs co-infected by both infectious agents). ten clinically healthy dogs were used as controls. a haematological profile was obtained and dog sera were assessed for the presence of platelet-bound igm and igg antibodies (pbigm, pbigg) using flow cytometry. eight of the naturally co-infected dogs (80%), five of the leishmaniotic dogs (50%) a ... | 2011 | 20456989 |
| first isolation and molecular characterization of ehrlichia canis in costa rica, central america. | the present study investigated ehrlichia species in blood samples from dogs suspected of clinical ehrlichiosis, using molecular and isolation techniques in cell culture. from a total of 310 canine blood samples analyzed by 16s rrna nested pcr, 148 (47.7%) were positive for ehrlichia canis. dna from ehrlichia chaffeensis or ehrlichia ewingii was not detected in any sample using species-specific primers in separated reactions. leukocytes from five pcr-positive dogs were inoculated into dh82 cells; ... | 2010 | 20723954 |
| evaluation of peptide- and recombinant protein-based assays for detection of anti-ehrlichia ewingii antibodies in experimentally and naturally infected dogs. [corrected] | to evaluate microtiter-plate format elisas constructed by use of different diagnostic targets derived from the ehrlichia ewingii p28 outer membrane protein for detection of e ewingii antibodies in experimentally and naturally infected dogs. | 2010 | 20919906 |
| seroprevalence of various infectious agents in dogs with suspected acute canine polyradiculoneuritis. | acute canine polyradiculoneuritis (acp) is considered to be an animal model of the acute axonal form of guillain-barré syndrome (gbs) in humans. various antecedent events have been associated with gbs, including bacterial or viral infection. the relationship between acp and previous infection requires additional attention. | 2011 | 21352374 |
| ehrlichiosis in brazil. | ehrlichiosis is a disease caused by rickettsial organisms belonging to the genus ehrlichia. in brazil, molecular and serological studies have evaluated the occurrence of ehrlichia species in dogs, cats, wild animals and humans. ehrlichia canis is the main species found in dogs in brazil, although e. ewingii infection has been recently suspected in five dogs. ehrlichia chaffeensis dna has been detected and characterized in mash deer, whereas e. muris and e. ruminantium have not yet been identifie ... | 2011 | 21439224 |
| exposure to infectious agents in dogs in remote coastal british columbia: possible sentinels of diseases in wildlife and humans. | ranked among the top threats to conservation worldwide, infectious disease is of particular concern for wild canids because domestic dogs (canis familiaris) may serve as sources and reservoirs of infection. on british columbia's largely undeveloped but rapidly changing central and north coasts, little is known about diseases in wolves (canis lupus) or other wildlife. however, several threats exist for transfer of diseases among unvaccinated dogs and wolves. to gain baseline data on infectious ag ... | 2011 | 21461190 |
| babesia canis rossi infection in a texas dog. | a 5-month-old intact male boerboel dog, imported from south africa 1 week previously, was presented to a texas veterinarian for lethargy, anorexia, and labored breathing. the dog was febrile, anemic, leukopenic, thrombocytopenic, and slightly azotemic. results of the idexx snap-4dx enzyme immunoassay were negative for dirofilaria immitis antigen and antibodies against ehrlichia canis, borrelia burgdorferi, and anaplasma phagocytophilum. an edta blood sample analyzed at oklahoma state university ... | 2011 | 21790699 |
| spatial distribution of seroprevalence for anaplasma phagocytophilum, borrelia burgdorferi, ehrlichia canis, and dirofilaria immitis in dogs in washington, oregon, and california. | background: in the us little spatially defined information regarding exposure to most vector-borne pathogens in dogs is available for the states of california (ca), oregon (or), and washington (wa). objectives: the purpose of the present study was to evaluate the spatial distribution of seroprevalence for 4 vector-borne pathogens, anaplasma phagocytophilum, borrelia burgdorferi, ehrlichia canis, and dirofilaria immitis, across the 3 western coastal states of the contiguous united states that ext ... | 2011 | 21827514 |
| retrospective evaluation of laboratory data on canine vector-borne infections from the years 2004-2008. | the detection and therapy of canine vector-borne diseases in imported dogs are of major importance in small animal practice. over the last years, the import of dogs from the mediterranean region and southeast europe has increased, countries often endemic for a variety of vector-borne diseases such as babesiosis, hepatozoonosis, leishmaniosis, dirofilariosis or ehrlichiosis. this retrospective study presents the evaluation of data from our diagnostic laboratory on vector-borne infections in impor ... | 2011 | 21950219 |
| asymptomatic macrothrombocytopenia in a young pure-bred beagle dog: a case report. | during baseline evaluation prior to a preclinical safety study, a 10-month-old male pure-bred beagle dog was found to have marked thrombocytopenia (6 × 10(3) platelets [plt]/µl) associated with a mean platelet volume (mpv) of 17.9 fl. tests for rickettsia rickettsii, ehrlichia canis, and borrelia burgdorferi were negative. buccal bleeding time was normal. over 3 months, plt were 4 to 141 × 10(3) plt/µl, and mpv was 11.4 to 25.1 fl; however, plt were <50 × 10(3) plt/µl and mpv was >16 fl during m ... | 2011 | 21859886 |
| Use of ELISA employing homologous and heterologous antigens for the detection of IgG and subclasses (IgG1 and IgG2) in the diagnosis of canine visceral leishmaniasis. | Indirect immunofluorescence is the method recommended for the diagnosis of visceral leishmanisis in dogs, however, the accuracy of this technique is low and its use on a large scale is limited. Since ELISA does not present these limitations, this technique might be an option for the detection of IgG or specific IgG1 and IgG2 subclasses. Canine ehrlichiosis is an important differential diagnosis of American Visceral Leishmaniasis (AVL). The present study compared ELISA using Leishmania chagasi an ... | 2011 | 22012455 |
| prevalence of selected zoonotic and vector-borne agents in dogs and cats in costa rica. | to estimate the prevalence of enteric parasites and selected vector-borne agents of dogs and cats in san isidro de el general, costa rica, fecal and serum samples were collected from animals voluntarily undergoing sterilization. each fecal sample was examined for parasites by microscopic examination after fecal flotation and for giardia and cryptosporidium using an immunofluorescence assay (ifa). giardia and cryptosporidium ifa positive samples were genotyped after pcr amplification of specific ... | 2011 | 21846585 |
| Typical and atypical manifestations of Anaplasma phagocytophilum infection in dogs. | Eighteen clinically ill dogs, naturally infected with Anaplasma phagocytophilum, were examined at a veterinary practice in Baxter, Minnesota. A clinical examination, complete blood cell count, enzyme- linked immunosorbent assay (ELISA) for A phagocytophilum, Borrelia burgdorferi, and Ehrlichia canis antibodies and Dirofilaria immitis antigen, and a polymerase chain reaction test for A phagocytophilum DNA were obtained for all dogs. Physical examination findings included fever, arthropathy, lymph ... | 2011 | 22058372 |
| contact with domestic dogs increases pathogen exposure in endangered african wild dogs (lycaon pictus). | infectious diseases have contributed to the decline and local extinction of several wildlife species, including african wild dogs (lycaon pictus). mitigating such disease threats is challenging, partly because uncertainty about disease dynamics makes it difficult to identify the best management approaches. serious impacts on susceptible populations most frequently occur when generalist pathogens are maintained within populations of abundant (often domestic) "reservoir" hosts, and spill over into ... | 2012 | 22238695 |
| first report of anaplasma platys infection in red foxes (vulpes vulpes) and molecular detection of ehrlichia canis and leishmania infantum in foxes from portugal. | the bacteria anaplasma platys and ehrlichia canis and the protozoan leishmania infantum are vector-borne agents that cause canine vector-borne diseases, some of which are zoonotic. the present survey investigated the prevalence of anaplasma, ehrlichia and leishmania in red foxes (vulpes vulpes) from portugal by molecular analysis, in order to evaluate the epidemiological role of these canids as reservoirs of infection. | 2015 | 25889750 |
| occurrence of tick-transmitted pathogens in dogs in jos, plateau state, nigeria. | canine babesiosis caused by babesia rossi, transmitted by haemaphysalis elliptica in south africa, has also been reported from nigeria. although h. leachi (sensu lato) is widespread in sub-saharan africa, published literature on the occurrence of canine babesiosis is meagre. it has been postulated that the genotype of babesia rossi erythrocyte membrane antigen 1 (brema1) may be linked to virulence of the specific isolate. the primary objective of this study was to detect and characterise tick-bo ... | 2014 | 24661795 |
| a molecular survey of vector-borne pathogens in red foxes (vulpes vulpes) from bosnia and herzegovina. | red foxes (vulpes vulpes) have recently been recognized as potential reservoirs of several vector-borne pathogens and a source of infection for domestic dogs and humans, mostly due to their close vicinity to urban areas and frequent exposure to different arthropod vectors. the aim of this study was to investigate the presence and distribution of babesia spp., hepatozoon canis, anaplasma spp., bartonella spp., 'candidatus neoehrlichia mikurensis', ehrlichia canis, rickettsia spp. and blood filaro ... | 2015 | 25889961 |
| molecular investigation of tick-borne pathogens in dogs from luanda, angola. | no molecular data have been available on tick-borne pathogens that infect dogs from angola. the occurrence of agents from the genera anaplasma, babesia, ehrlichia and hepatozoon was assessed in 103 domestic dogs from luanda, by means of the polymerase chain reaction (pcr) and dna sequence analysis. | 2016 | 27160839 |
| molecular detection of tick-borne rickettsial and protozoan pathogens in domestic dogs from turkey. | canine tick-borne parasites have emerged in recent years, showing a wider geographic distribution and increased global prevalence. in addition to their veterinary importance, domestic dogs play an important role in the transmission cycles of some agents by acting as reservoirs and sentinels. this study investigated babesia, theileria, anaplasma, and ehrlichia species in asymptomatic dogs in ten provinces of turkey. | 2015 | 25889911 |
| molecular detection of zoonotic rickettsiae and anaplasma spp. in domestic dogs and their ectoparasites in bushbuckridge, south africa. | members of the order rickettsiales are small, obligate intracellular bacteria that are vector-borne and can cause mild to fatal diseases in humans worldwide. there is little information on the zoonotic rickettsial pathogens that may be harbored by dogs from rural localities in south africa. to characterize rickettsial pathogens infecting dogs, we screened 141 blood samples, 103 ticks, and 43 fleas collected from domestic dogs in bushbuckridge municipality, mpumalanga province of south africa, be ... | 2016 | 26974185 |
| a survey of tick-borne pathogens in dogs and their ticks in the pantanal biome, brazil. | tick and blood samples collected from domestic dogs in the brazilian pantanal were tested by molecular methods for the presence of tick-borne protozoa and bacteria. among 320 sampled dogs, 3.13% were infected by babesia vogeli (piroplasmida: babesiidae), 8.75% by hepatozoon canis (eucoccidiorida: hepatozoidae), 7.19% by anaplasma platys (rickettsiales: anaplasmataceae), and 0.94% by an unclassified anaplasma sp. in three tick species collected from dogs, the following tick-borne agents were dete ... | 2016 | 26467462 |
| prevalence and diversity of babesia, hepatozoon, ehrlichia, and bartonella in wild and domestic carnivores from zambia, africa. | a molecular survey was conducted for several hemoparasites of domestic dogs and three species of wild carnivores from two sites in zambia. three babesia spp. were detected including babesia felis and babesia leo in lions (panthera leo) and a babesia sp. (similar to babesia lengau) in spotted hyenas (crocuta crocuta) and a single lion. all wild dogs (lycaon pictus) and domestic dogs were negative for babesia. high prevalences for hepatozoon were noted in all three wild carnivores (38-61%) and in ... | 2014 | 24363181 |
| molecular detection of vector-borne pathogens in wild and domestic carnivores and their ticks at the human-wildlife interface. | urbanization of natural areas is considered one of the causes of the current apparent emergence of infectious diseases. carnivores are among the species that adapt well to urban and periurban environments, facilitating cross-species disease transmission with domestic dogs and cats, and potentially with their owners. the prevalence of vector-borne pathogens (vbp) of zoonotic and veterinary interest was studied in sympatric wild and domestic carnivores into barcelona metropolitan area (ne spain). ... | 2016 | 26643497 |
| ferritin 1 silencing effect in rhipicephalus sanguineus sensu lato (acari: ixodidae) during experimental infection with ehrlichia canis. | rhipicephalus sanguineus sensu lato (s.l.) is a very common ectoparasite of domestic dogs able to transmit several pathogens of human and veterinary importance. tick infestations and tick-borne diseases (tbds) remain a serious and persistent problem, due to the lack of efficient control measures. it is therefore vital that novel approaches to control are pursued. whilst vaccination is recognised as a potential control method to reduce tick infestation, no anti-r. sanguineus vaccine is available. ... | 2017 | 27825659 |
| molecular detection of rangelia vitalii in domestic dogs from uruguay. | the piroplasm rangelia vitalii is the etiological agent of canine rangeliosis, a severe disease affecting domestic dogs in south america. two domestic dogs from two different departments (salto and treinta y tres) of uruguay presented with clinical signs such as apathy, anorexia, pale mucous membranes, jaundice, and hemorrhagic manifestations, suggestive of a canine vector-borne disease. molecular analysis, based on pcr and dna sequencing of portions of the 18s rrna gene, revealed that both dogs ... | 2015 | 25843009 |
| molecular detection and treatment of tick-borne pathogens in domestic dogs in khon kaen, northeastern thailand. | we determined the prevalence of tick-borne pathogens in domestic dogs using microscopy and polymerase chain reaction (pcr) techniques. a total of 303 edta blood samples were collected from domestic dogs in khon kaen province, thailand, in may 2013. microscopic observation of giemsa-stained smears and molecular diagnosis using conventional pcr were performed. infected dogs were treated with imidocarb dipropionate, a combination of imidocarb dipropionate and doxycycline, or doxycycline alone. seve ... | 2014 | 25417519 |
| first detection and molecular characterization of ehrlichia canis from dogs in nigeria. | the present study aimed to detect the presence of ehrlichia canis in naturally infected dogs in nigeria, using a combination of pcr and sequence analysis of the 16s rrna gene and two genes encoding the tandem repeat-containing proteins (trps), trp19 and trp36. out of a total of 100 blood samples collected from domestic dogs presented to veterinary hospitals in jos, the capital city of plateau state of nigeria, 11 were positive in nested pcr for e. canis. sequencing results for these amplicons sh ... | 2013 | 22925936 |
| molecular detection of vector-borne pathogens in dogs and cats from qatar. | vector-borne diseases (vbds) have been increasingly reported in dogs and cats worldwide. however, no data are currently available regarding canine and feline vbds in qatar and limited information is available from other persian gulf countries. | 2017 | 28633666 |
| molecular prevalence and haemato-biochemical profile of canine monocytic ehrlichiosis in dogs in and around hisar, haryana, india. | the present study was planned to investigate the molecular prevalence of canine monocytic ehrlichiosis (cme) in dogs in and around hisar and to evaluate the haemato-biochemical profile for its better management. a total of 60 dogs presented to medicine section, tvcc, luvas, hisar with the history of naturally acquired tick infestation and clinical signs consistent with cme were screened on the basis of blood smear examination, followed by molecular detection by nested pcr assay targeting a porti ... | 2017 | 28848253 |