Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [the demonstration of vaccinia virus in the spinal cord of young cattle]. | 1952 | 13006751 | |
| milker's nodules: isolation of a poxvirus from a human case. | a virus of the pox group was isolated from a lesion on a patient with milker's nodules by use of tissue cultures of bovine cells. the virus shows marked biologic and serologic differences from vaccinia virus, and it is presumably the etiologic agent of the milker's nodule syndrome. | 1963 | 13959825 |
| isolation of a viral agent from pseudocowpox disease. | a virus with characteristics similar to the pox group was isolated in tissue culture from bovine skin and oral lesions typical of pseudocowpox. the properties of this agent, certain of which differ from vaccinia virus, suggest that the isolate is the etiologic agent of the pseudocowpox syndrome. | 1963 | 14043336 |
| isolation of a paravaccinia virus from a cow in japan. | 1967 | 5624717 | |
| the neutralization of pox viruses. ii. relationships between vaccinia, rabbitpox, cowpox and ectromelia. | 1968 | 5250179 | |
| [isolation and characterization of cowpox virus]. | 1970 | 5537837 | |
| letter: epidemiology of cowpox. | 1975 | 1167474 | |
| a method for serological differentation of closely related poxviruses. | a two-step gel precipitation reaction with antiserum to vaccinia virus, based on sequential addition of two test viruses was elaborated for differentiation of closely related orthopoxviruses. the appearance of an additional precipitation line indicated differences in the antigenic structure of the viruses compared. differences in the antigenic structure of vaccinia, variola and monkey pox viruses as well as the antigenic identity with cow pox virus of poxviruses isolated from carnivora, elephant ... | 1976 | 61718 |
| purification of mrna guanylyltransferase from calf thymus. | mrna guanylyltransferase has been extensively purified from calf thymus. a gtp-binding assay was used based on the observations by shuman and hurwitz (1981) and venkatesan and moss (1982) that vaccinia virus and hela cell mrna guanylyltransferases bind the gmp moiety from gtp in the absence of an acceptor rna. the mol. wt. of the purified enzyme from calf thymus, estimated by polyacrylamide gel electrophoresis in the presence of sds, is 65 000. the major protein in the purified enzyme fraction c ... | 1982 | 6329686 |
| carbocyclic analogue of 3-deazaadenosine: a novel antiviral agent using s-adenosylhomocysteine hydrolase as a pharmacological target. | the carbocyclic analogue of 3-deazaadenosine (3-deaza-c-ado) has been synthesized and found to have antiviral activity in cell culture against herpes simplex virus type 1, vaccinia virus, and hl-23 c-type virus. it is relatively noncytotoxic at effective antiviral concentrations and is not subject to deamination or phosphorylation. it acts as a competitive inhibitor of s-adenosyl-l-homocysteine hydrolase, is at best a poor substrate, and does not inactivate the enzyme significantly. 3-deaza-c-ad ... | 1982 | 7097716 |
| natural and experimental pox virus infection in the domestic cat. | three naturally occurring cases of cowpox virus infection in the domestic cat are described. isolate l97 was identified as cowpox virus on the basis of morphology, serology and characteristic cytopathic effect in tissue culture and on the chorioallantoic membrane of embryonated eggs. all three cases showed multiple skin lesions, slight conjunctivitis or purulent ocular discharge but there were no respiratory signs. two animals recovered, the third was put down as a stray. the disease was reprodu ... | 1983 | 6836897 |
| expression vehicles used in recombinant dna technology. | we survey cloning vehicles whose function is to carry and express a gene in host cells including escherichia coli, saccharomyces cerevisiae and mammalian cells. in e. coli these include vehicles based on the lac operon, the trp operon, the rho leftward operon, and the reca gone; open reading frame cloning vehicles are also discussed, as are steps that can be taken to extrude a gene product from the cell and the use of plasmids with runaway replication. in s. cerevisiae we discuss vehicles based ... | 1983 | 14540892 |
| neplanocin a. a potent inhibitor of s-adenosylhomocysteine hydrolase and of vaccinia virus multiplication in mouse l929 cells. | neplanocin a, a novel cyclopentenyl analog of adenosine, is a naturally occurring antibiotic which exhibits significant antitumor activity against l1210 leukemia in mice (yaginuma, s., muto, n., tsujino, m., sudate, y., hayashi, m., and otani, m. (1981) j. antibiot. 34, 359-366). in the present study we demonstrate that neplanocin a is also a potent inhibitor of s-adenosylhomocysteine (adohcy) hydrolase (ec 3.3.1.1) having a ki of 8.39 nm for the purified bovine liver enzyme. analysis of the app ... | 1984 | 6707008 |
| new outlook on the biology of cowpox virus. | analysis of cowpox outbreaks revealed an extremely wide range of virus pathogenicity including 9 orders of mammals. recent serological and virological data support the hypothesis that wild rodents may be a natural reservoir of cowpox virus. cowpox infection of humans occurring without any contact with infected cattle (registered in the u. k. and poland) is especially interesting for medical and veterinary virology. surveillance seems justified of the possible virus dissemination beyond its natur ... | 1984 | 6151360 |
| resistance of vaccinia virus to interferon is related to an interference phenomenon between the virus and the interferon system. | in this investigation the sensitivity of vaccinia virus to interferon (ifn) has been examined in cultured cells. in a variety of mouse and human cells of different origins vaccinia virus functions (rna, protein, and virus yields) were found to be relatively resistant to ifn. in these systems, the levels of the ifn-mediated enzyme activities (2-5a synthetase and protein kinase) were severely impaired by the virus. this virus-mediated inhibitory effect developed with time after infection and was d ... | 1984 | 6324465 |
| structural and functional properties of dna polymerase delta from rabbit bone marrow. | dna polymerase delta, the most recently described class of eukaryotic dna polymerase, has been purified to apparent homogeneity from rabbit bone marrow. unlike the previously known eukaryotic dna polymerases, delta has a 3' to 5' exonuclease as an integral component of its 122 000 molecular weight, single polypeptide structure. similar to the function with prokaryotic dna polymerases, the 3' to 5' exonuclease assists dna polymerase delta in maintaining the fidelity of dna synthesis by excising m ... | 1984 | 6330522 |
| vaccinia virus 19-kilodalton protein: relationship to several mammalian proteins, including two growth factors. | we observed two unusual patterns of cysteine and glycine residues in transforming growth factor type 1 (tgf1); our computer search found only one other protein, the 19-kilodalton early protein of vaccinia virus, having both patterns. the sequences of epidermal growth factor (egf) and of the light chains from several components of the blood coagulation system also have one of the patterns, but gaps are required to adjust conserved cysteine and glycine residues in the second pattern. we used sever ... | 1984 | 6334307 |
| similarity between the vaccinia virus 19k early protein and epidermal growth factor. | an analysis of the 1,217-amino acid residue sequence of the precursor of mouse epidermal growth factor (megf) revealed regions of considerable similarity with bovine factor x, a blood coagulation factor. similarities of megf itself with factor x, pancreatic secretory trypsin inhibitor and, most strikingly, transforming growth factor i (tgf-i) have been observed. on the basis of the comparisons described here, it seems that the presumptive 140-residue 19k early protein (relative molecular mass (m ... | 1985 | 3871916 |
| potential inhibitors of s-adenosylmethionine-dependent methyltransferases. 8. molecular dissections of carbocyclic 3-deazaadenosine as inhibitors of s-adenosylhomocysteine hydrolase. | a series of 9-(hydroxyalkyl)-3-deazaadenines, which are analogues of the carbocyclic derivative of 3-deazaadenosine (3-deaza-c-ado), were synthesized. the analogues were tested as inhibitors of bovine liver s-adenosyl-l-homocysteine (adohcy) hydrolase (ec 3.3.1.1) and as inhibitors of vaccinia virus (wr) replication in clone 929 mouse l cells and the results were compared to those observed for the parent compound, 3-deaza-c-ado. 4-amino-1-(2,3-dihydroxy-1-propyl)imidazo[4,5-c]pyridine (14), the ... | 1985 | 3981538 |
| potential inhibitors of s-adenosylmethionine-dependent methyltransferases. 9. 2',3'-dialdehyde derivatives of carbocyclic purine nucleosides as inhibitors of s-adenosylhomocysteine hydrolase. | a series of purine (e.g., adenine, n6-methyladenine, 8-azaadenine, 3-deazaadenine) carbocyclic nucleosides, nucleoside 2',3'-dialdehydes, and nucleoside 2',3'-diols were synthesized as potential inhibitors of bovine liver s-adenosyl-l-homocysteine (adohcy) hydrolase (ec 3.3.1.1) and as potential inhibitors of vaccinia virus replication. the 2',3'-dialdehydes were prepared by periodate oxidation of the corresponding carbocyclic nucleosides. reduction of the intermediate dialdehydes with sodium bo ... | 1985 | 3981539 |
| vaccinia virus recombinants: expression of vsv genes and protective immunization of mice and cattle. | vesicular stomatitis virus (vsv) causes a contagious disease of horses, cattle, and pigs. when dna copies of messenger rna's for the g or n proteins of vsv were linked to a vaccinia virus promoter and inserted into the vaccinia genome, the recombinants retained infectivity and synthesized vsv polypeptides. after intradermal vaccination with live recombinant virus expressing the g protein, mice produced vsv-neutralizing antibodies and were protected against lethal encephalitis upon intravenous ch ... | 1985 | 2981435 |
| expression and regulation of the vaccinia virus thymidine kinase gene in non-permissive cells. | the expression and regulation of the vaccinia virus (vv) thymidine kinase (tk) gene was examined in two non-permissive cell lines, cho and mdbk, which restrict vv development at different stages of the viral replication cycle. the vv tk gene was expressed in these two cell lines with kinetics similar to a fully permissive cell line bsc40. these results are consistent with the hypothesis that inhibition of tk mrna translation by another viral early gene product is a normal component of the overal ... | 1985 | 4024727 |
| antiviral effect of bacterially produced human interferon (hu-ifn alpha 2) against experimental vaccinia infection in calves. | the heterologous antiviral efficiency of bacterially produced human interferon (hu-ifn alpha 2) in the bovine species was studied, using vaccinia infection as experimental model. in a double blind experiment, young calves were intramuscularly injected daily for seven consecutive days with different doses of hu-ifn alpha 2 or placebo, the treatment starting 24 h before intradermal inoculation of vaccinia virus. a clear protection by interferon was observed in all the ifn treated animals, although ... | 1985 | 3989335 |
| immunisation of cattle with a recombinant togavirus-vaccinia virus strain. | genetic engineering techniques have been used to construct a vaccinia virus recombinant which contains and expresses togavirus (sindbis) genetic information. intradermal inoculation of this recombinant strain into calves caused a transient pock-type lesion at the site of inoculation and elicited the production of substantial levels of anti-sindbis virus neutralising antibodies. these results suggest that recombinant vaccinia virus vaccines may have potential for use in veterinary medicine. | 1985 | 3862196 |
| new technology for prevention and control of infectious bovine respiratory diseases. | recent advances in molecular biology, genetics, and immunochemistry have provided the basis for a new generation of vaccines, diagnostic tools, and prophylactic substances for the prevention of infectious diseases in cattle. this article describes how these new technologies are being applied to veterinary medicine and their potential in bovine respiratory diseases caused by infectious agents. | 1985 | 2416411 |
| response of dairy calves to vaccinia viruses that express foreign genes. | repeated intradermal inoculations of calves with wild-type vaccinia virus and recombinant vaccinia viruses expressing human hepatitis b virus surface antigen and herpes simplex virus, type 1, glycoprotein d produced characteristic pox lesions at each site of injection. in some instances, calves were inoculated as many as five times at intervals from 4 to 7 weeks. the lesions invariably were more severe after the second inoculation. subsequent inoculations produced a less severe area of redness, ... | 1986 | 3700615 |
| [viral vaccines produced by gene technology and synthesis]. | the gradual evolution of vaccine development from jenner's smallpox vaccine to recombinant genetechnology has provided the basis for prophylactic control of a lot of important infectious diseases of humans and animals. modern biotechnology has accomplished antigen identification and isolation of antigen determinants as well as the preparation of proteins and synthetic peptides. the application of such techniques has failed, however, to develop a better vaccine in case of fmd-virus. present effor ... | 1986 | 2424142 |
| responses of cattle, sheep and poultry to a recombinant vaccinia virus expressing a swine influenza haemagglutinin. | groups of cattle, sheep and poultry were inoculated with a recombinant vaccinia virus expressing the haemagglutinin of the swine influenza virus a/nj/11/76. no adverse clinical responses were recorded and none of the animals developed a viraemia when inoculated with the recombinant or wild-type vaccinia virus. recombinant virus reisolated from lesions in cattle was stable, maintaining its thymidine kinase negative phenotype and ability to express the swine influenza haemagglutinin. antibodies to ... | 1986 | 3764100 |
| recombinant vaccinia viruses as vaccines. | the prospect of live vaccines consisting of genetically modified vaccinia virus expressing foreign genes is exciting, but important issues concerning safety and efficacy need to be resolved. | 1986 | 3945343 |
| progress towards control of the acute respiratory viral diseases of childhood. | many of the common respiratory illnesses of infancy and childhood are caused by viruses of the paramyxoviridae family, in particular measles virus, respiratory syncytial (rs) virus and parainfluenzavirus type 3 (pi3). effective measles vaccine was developed by classical methods, but these same methods have failed to provide vaccines to control rs and pi3 virus infections. the who programme for vaccine development was initiated in 1983 to encourage the application of the new biotechnologies to co ... | 1987 | 3496981 |
| analysis of the l1 gene product of human papillomavirus type 16 by expression in a vaccinia virus recombinant. | the l1 open reading frame of human papillomavirus type 16 (hpv16) has been expressed in vaccinia virus under the control of both the 7.5k early and late promoter, and the 4b major late promoter. antibodies to a beta-galactosidase fusion protein containing a c-terminal portion of the hpv16 l1 gene product were used to compare the levels of l1 expression in the two recombinants, and showed that greater levels of expression were obtained when the gene was placed under the control of the 4b late pro ... | 1988 | 2838573 |
| protection of cattle against rinderpest with vaccinia virus recombinants expressing the ha or f gene. | rinderpest is a highly contagious ruminant viral disease manifested by a rapid course and greater than 90% mortality. infectious vaccinia virus recombinants were constructed that express either the hemagglutinin or the fusion gene of rinderpest virus. all cattle vaccinated with either recombinant or with the combined recombinants produced neutralizing antibodies against rinderpest virus and were protected against the disease when challenged with more than 1000 times the lethal dose of the virus. | 1988 | 3194758 |
| immunogenicity, pathogenicity, and transmissibility of a recombinant vaccinia virus in calves. | experiments concerned with the immunogenicity, pathogenicity, and transmissibility of a recombinant vaccinia:sindbis virus were conducted. the wr strain of the recombinant vaccinia:sindis virus was found to be infective for calves and mildly pathogenic, resulting in local tissue reaction. it was not transmissible to other calves. also, it was found to be immunogenic when inoculated intradermally into calves, and antibody was produced against the parent vector virus (vaccinia) and the sindbis ant ... | 1988 | 3239835 |
| potential inhibitors of s-adenosylmethionine-dependent methyltransferases. 11. molecular dissections of neplanocin a as potential inhibitors of s-adenosylhomocysteine hydrolase. | a series of 9-(hydroxyalkenyl)purines (adenines and 3-deazaadenines), which are analogues of neplanocin a, were synthesized. the analogues were tested as inhibitors of bovine liver and murine l929 cell s-adenosyhomocysteine (adohcy) hydrolase (ec 3.3.1.1) and as inhibitors of vaccinia virus replication in murine l929 cells. compounds 1b, 2a, 2b, 4a, 4b, 7, 9a, and 9b showed the best inhibitory effects toward bovine liver adohcy hydrolase, with compound 4b being the most potent. the compounds tha ... | 1988 | 3411600 |
| immunogenicity of a recombinant vaccinia virus expressing envelope a glycoprotein of bovine leukaemia virus. | we constructed a recombinant vaccinia virus (rvv) expressing envelope (env) glycoprotein (gp51) of bovine leukaemia virus (blv): the expression of gp51 was detected by western blot of the lysates of rabbit kidney cells infected with the rvv. the rabbits inoculated intradermally with the rvv alone failed to induce detectable anti-gp51 antibodies even 10 weeks after immunization. however, when these animals were boosted with inactivated blv virion in saline, significant levels of anti-gp51 antibod ... | 1988 | 2848378 |
| immune response and protection of cattle and pigs generated by a vaccinia virus recombinant expressing the f protein of rinderpest virus. | the immune response of cattle and pigs to a vaccinia recombinant virus containing the fusion (f) protein gene of rinderpest virus was examined. half the cattle and all the pigs gave humoral response to primary vaccination and all the cattle gave an anamnestic response to a second vaccination 28 days after the primary vaccination. all the cattle after a single or secondary vaccination were completely protected clinically after exposure to a lethal dose of the saudi 1/81 strain of virus. prior vac ... | 1989 | 2763430 |
| protection of cattle from infection with vaccinia virus by bovine interferon alpha c. | the efficacy of bovine interferon alpha c as an antiviral compound was evaluated in calves infected with vaccinia virus. calves treated with the interferon did not develop characteristic vaccinia lesions, demonstrating a clear protective effect of treatment. the only side effect observed was hyperthermia. after intramuscular injection, interferon appeared quickly in the blood and was cleared within 24 hours. the in vivo antiviral activity of bovine interferon alpha c was confirmed by its ability ... | 1989 | 2781688 |
| expression of the bovine leukemia virus transactivator protein p34 by a recombinant vaccinia virus. | in order to characterize the bovine leukemia virus transactivator protein, a recombinant vaccinia virus (v-lor) containing the blv post-envelope long open reading frame was constructed. v-lor was shown to encode a functional protein able to transactivate the blv long terminal repeat-directed gene expression in the infected cells. the encountered level of transactivation was about one third of that measured in blv-infected fetal lamb kidney cell lysates. | 1989 | 2560852 |
| [rabies, a public health problem]. | in west-europe, rabies is mostly reported in foxes, and in less extend in different domestic animals including dogs, cats, cattle, sheep, goats and horses. the contamination to humans occurs through contact with rabid animals by bite, scratch and even contact with the wound. this paper reports upon the current situation of rabies disease in belgium and the prophylactic means routinely applied and those actually under experiment. in 1988, 514 animals were found positive, among which 366 foxes (71 ... | 1989 | 2610551 |
| synthesis, cellular location, and immunogenicity of bovine herpesvirus 1 glycoproteins gi and giii expressed by recombinant vaccinia virus. | two of the major glycoproteins of bovine herpesvirus 1 (bhv-1) are gi, a polypeptide complex with apparent molecular weights of 130,000, 74,000, and 55,000, and giii (a 91,000-molecular-weight [91k] glycoprotein), which also exists as a 180k dimer. vaccinia virus (vac) recombinants were constructed which carry full-length gi (vac-i) or giii (vac-iii) genes. the genes for gi and giii were each placed under the control of the early vac 7.5k gene promoter and inserted within the vac gene for thymid ... | 1989 | 2539509 |
| production of pro-opiomelanocortin (pomc) by a vaccinia virus transient expression system and in vitro processing of the expressed prohormone by pomc-converting enzyme. | pro-opiomelanocortin (pomc) was expressed in cv-1 (green monkey kidney) cells using a vaccinia virus transient expression system [(1986) proc. natl. acad. sci. usa 83, 8122]. the system involved infection of cells with a recombinant vaccinia virus carrying the t7 rna polymerase gene and transfection with a plasmid containing the mouse pomc sequence flanked by the t7 rna polymerase promoter at its 5'-end and the t7 rna polymerase terminator at its 3'-end. assay of the medium from transfected cell ... | 1989 | 2542089 |
| syncytium formation by recombinant vaccinia viruses carrying bovine parainfluenza 3 virus envelope protein genes. | the highly syncytium-inducing m strain and the weakly syncytium-inducing sc strain of bovine parainfluenza 3 virus differ by a single amino acid substitution in each of the hemagglutinin-neuraminidase (hn) and membrane (m) proteins, while their fusion (f) proteins are identical (t. shioda, s. wakao, s. suzu, and h. shibuta, virology 162:388-396, 1988). we constructed recombinant vaccinia viruses which express separately the m virus hn (vac-mhn), sc virus hn (vac-schn), m virus m (vac-mm), sc vir ... | 1989 | 2547990 |
| prospects for the total eradication of rinderpest. | rinderpest, a major economic problem of africa and asia, is a highly contagious viral disease of cattle and buffalo with greater than 90% mortality in susceptible herds. we have developed an effective vaccinia virus recombinant vaccine for rinderpest which protects cattle against challenge by more than 1000 times the lethal dose of the virus. the vaccine circumvents the major logistical problems of the plowright tissue culture vaccine that occasioned previous failures to eradicate rinderpest by ... | 1989 | 2609722 |
| development of recombinant antigen vaccines for the control of theileriosis. | immunization against theileria parva involves infection with sporozoites and simultaneous treatment with a long-acting tetracycline. for t. annulata, immunization is achieved by inoculation of attenuated schizont-infected lymphocytes. the two methods are inadequate because of the use of live organisms and the methods are also bedevilled by the multiplicity of strains, particularly of t. parva. for these reasons, alternative methods of control are being sought. in this review an attempt is made t ... | 1990 | 2149448 |
| bovine dopamine beta-hydroxylase cdna. complete coding sequence and expression in mammalian cells with vaccinia virus vector. | we have isolated cdna clones for bovine dopamine beta-hydroxylase from an adrenal medulla cdna library and have determined the complete coding sequence. the largest cdna clone isolated from the library is 2.4 kilobase pairs (kb) and contains an open reading frame of 1788 bases, coding for a protein of 597 amino acids and mr = 66,803. the predicted amino acid sequence of the bovine cdna contains 85% identity with human dopamine beta-hydroxylase (lamouroux, a., vingny, a., faucon biquet, n., darmo ... | 1990 | 1688549 |
| inhibition of antigen-induced and interleukin-2-induced proliferation of bovine peripheral blood leukocytes by inactivated bovine herpes virus 1. | the mechanism by which bovine herpesvirus 1 (bhv-1) predisposes cattle to bacterial pneumonia was investigated by using an in vitro system to demonstrate immunosuppression. at a multiplicity of infection of 0.001, live or inactivated bhv-1 induced a 50% inhibition of the proliferative response of peripheral blood mononuclear leukocytes to antigen (vaccinia virus in vaccinia virus-immunized cattle which were bhv-1 negative) or interleukin-2. at this same multiplicity of infection, the mitogen-ind ... | 1990 | 2166810 |
| induction of neutralizing antibodies against bovine leukosis virus in rabbits by vaccination with recombinant vaccinia virus expressing bovine leukosis virus envelope glycoprotein. | three kinds of recombinant vaccinia virus (rvv)--mo-ha/ati, lo1-ha/ati and mo-ha/7.5kd--expressing bovine leukosis virus (blv) envelope glycoprotein (gp60) were constructed. the blv envelope gene of rvv mo-ha/ati and lo1-ha/ati or of rvv mo-ha/7.5kd was expressed under control of the promoter of a-type inclusion body (ati) protein gene of cow-pox virus or vaccinia virus 7.5-kd protein gene, respectively. the vaccinia virus strain, lc16mo, was used as vector for rvv mo-ha/ati and mo-ha/7.5kd, and ... | 1990 | 2167028 |
| complementation of a vesicular stomatitis virus glycoprotein g mutant with wild-type protein expressed from either a bovine papilloma virus or a vaccinia virus vector system. | using a complementation assay, we have evaluated the potential of two eukaryotic expression systems to produce functional virus proteins. the first expression system was based on a bovine papilloma virus (bpv) eukaryotic expression vector which contained a copy of the gene for the membrane glycoprotein g of vesicular stomatitis virus (vsv). this vector was transfected into a mouse cell line, and transformed cell clones constitutively expressing vsv g protein were selected. these cell clones were ... | 1990 | 2171187 |
| proteins encoded by the bovine papillomavirus e1 open reading frame: expression in heterologous systems and in virally transformed cells. | the e1 open reading frame (orf) of bovine papillomavirus type 1 is required for the persistence of viral genomes as multicopy plasmid molecules in transformed rodent fibroblasts. e1 has been reported to contain two separate complementation groups (m and r, corresponding to n- and c-terminal domains, respectively) which regulate viral replication. however, e1 behaves as a single gene with respect to cell transformation and viral transcription. we examined the proteins translated from the entire o ... | 1990 | 2173778 |
| vaccinia recombinants expressing early bovine papilloma virus (bpv1) proteins: retardation of bpv1 tumour development. | papillomaviruses are aetiological agents of epithelial proliferative diseases in animals and in man. it was previously demonstrated that animals inoculated with live vaccinia recombinants expressing early proteins of polyoma virus resist challenge with polyoma-tumour cells, and this approach has been extended to the development of a vaccine against papillomavirus-transformed cells. bovine papillomavirus type 1 (bpv1), a virus responsible for dermal lesions in cattle, is a prototype virus of the ... | 1990 | 2163573 |
| nucleotide sequence analysis and expression from recombinant vectors demonstrate that the attachment protein g of bovine respiratory syncytial virus is distinct from that of human respiratory syncytial virus. | bovine respiratory syncytial (brs) virus causes a severe lower respiratory tract disease in calves similar to the disease in children caused by human respiratory syncytial (hrs) virus. while there is antigenic cross-reactivity among the other major viral structural proteins, the major glycoprotein, g, of brs virus and that of hrs virus are antigenically distinct. the g glycoprotein has been implicated as the attachment protein for hrs virus. we have carried out a molecular comparison of the glyc ... | 1990 | 2214024 |
| cleavage of proenkephalin by a chromaffin granule processing enzyme. | human proenkephalin generated by means of a recombinant vaccinia virus expression vector was used as the substrate for a putative processing enzyme obtained from bovine adrenal chromaffin granules. the adrenal enzyme successfully cleaved proenkephalin to generate low mol wt enkephalins as well as other enkephalin-containing intermediates. radioactively labeled proenkephalin prepared with this system was also cleaved; however, under identical conditions bovine proinsulin was not cleaved. these re ... | 1990 | 2294000 |
| development of a specific serological test and an efficient subunit vaccine to control bovine leukemia virus infection. | study of the antigenic structure of the bovine leukemia virus (blv) envelope glycoprotein gp51 with a panel of mouse monoclonal antibodies (mabs) has allowed the identification of biologically important determinants directly involved in the infectivity of blv. considering the various facts reported in this paper, it follows that diagnostic and vaccination procedures that make use of gp51 in a native configuration constitute a prerequisite for the design of an efficient blv eradication program. t ... | 1990 | 1704325 |
| mammalian dna ligases. catalytic domain and size of dna ligase i. | dna ligase i is the major dna ligase activity in proliferating mammalian cells. the protein has been purified to apparent homogeneity from calf thymus. it has a monomeric structure and a blocked n-terminal residue. dna ligase i is a 125-kda polypeptide as estimated by sodium dodecyl sulfate-gel electrophoresis and by gel chromatography under denaturing conditions, whereas hydrodynamic measurements indicate that the enzyme is an asymmetric 98-kda protein. immunoblotting with rabbit polyclonal ant ... | 1990 | 1695631 |
| host cell-dependent lateral mobility of viral glycoproteins. | the lateral mobility of viral envelope proteins on the plasma membranes of infected cells is an important factor in both virus assembly and pathogenesis. the envelope glycoproteins of measles and human parainfluenza virus are mobile on the surfaces of infected hela cells and undergo lateral redistribution in the presence of specific antibody, forming unipolar caps. in contrast, no such redistribution was observed with influenza virus hemagglutinin (ha) or vesicular stomatitis virus (vsv) g glyco ... | 1990 | 1965847 |
| expression and processing of the activin-a/erythroid differentiation factor precursor: a member of the transforming growth factor-beta superfamily. | the biosynthesis and intracellular processing of the polypeptide precursor of the beta a-chain of the fertility hormone inhibin were assessed by infecting a wide spectrum of cell types with a recombinant vaccinia virus. most cell lines, including follicular granulosa cells, secrete both prohormone and mature hormone as homodimers (activin) composed of disulfide-linked subunits of 54 kda (proactivin-a) and 14 kda (activin-a), respectively, and a small amount of prohormone-mature hormone heterodim ... | 1990 | 1963471 |
| expression of bovine leukaemia virus envelope gene by recombinant vaccinia viruses. | recombinant vaccinia viruses (vv) containing the envelope gene of bovine leukaemia virus (blv) were constructed. three virus constructs were designed: vv-blv1 which contained the open reading frame for envelope glycoprotein gp51 alone, under control of vvp7.5 promoter; vv-blv2 and vv-blv3 contained the entire gene (gp51 + gp30) coding sequence downstream of vp7.5 and the fowlpox virus early/late promoter (pfe/l) respectively. all three vv recombinants expressed envelope glycoproteins as determin ... | 1990 | 1963249 |
| nucleotide sequence analysis of the bovine respiratory syncytial virus fusion protein mrna and expression from a recombinant vaccinia virus. | bovine respiratory syncytial (brs) virus is an important cause of serious respiratory illness in calves. the disease caused in calves is similar to that caused by human respiratory syncytial (hrs) virus in children. the two viruses, however, have distinct host ranges and the attachment glycoproteins, g, have no antigenic cross-reactivity. the fusion glycoproteins, f, of the hrs and brs viruses, however, have some antigenic cross-reactivity. to further compare the brs virus and hrs virus fusion p ... | 1991 | 1994571 |
| conglutinin binds the hiv-1 envelope glycoprotein gp 160 and inhibits its interaction with cell membrane cd4. | the highly glycosylated envelope glycoprotein (gp 160) of human immunodeficiency virus (hiv) interacts with the cd4 molecule present on the membrane of cd4+ cells and is involved in the pathobiology of hiv infection. lectins bind glycoproteins through non-covalent interactions with specific hexose residues. the mammalian c-type lectin bovine conglutinin was examined for its ability to interact with recombinant gp160 (rgp160) produced in vaccinia virus-infected bhk21 cells. specific binding of co ... | 1991 | 1996409 |
| a vaccinia virus double recombinant expressing the f and h genes of rinderpest virus protects cattle against rinderpest and causes no pock lesions. | rinderpest is a highly contagious viral disease of ruminants with greater than 95% morbidity and mortality. we have constructed an infectious vaccinia virus recombinant that expresses both the fusion (f) gene and the hemagglutinin (h) gene of rinderpest virus. the wyeth strain of vaccinia virus was used for the construction of the recombinant. cattle vaccinated with the recombinant virus were 100% protected from challenge inoculation with greater than 1000 times the lethal dose of rinderpest vir ... | 1991 | 1896447 |
| expression and processing of mouse proopiomelanocortin in bovine adrenal chromaffin cells. a model system to study tissue-specific prohormone processing. | many neuroendocrine precursor proteins, such as proopiomelanocortin (pomc), are cleaved in a tissue specific manner at distinct pairs of basic amino acids. elucidating the specificity of the prohormone endoprotease(s) is essential to understanding cleavage specificity. however, isolation of these enzymes has been difficult, due to the inability to distinguish authentic maturation enzyme from the many other trypsin-like activities present in tissue homogenates. recently, a "signature" of the insu ... | 1991 | 1856197 |
| [synthesis of the e-antigen of the hepatitis b virus (hbeag) in eukaryotic cells by a recombinant strain of the vaccinia virus]. | the recombinant plasmids containing the gene for hepatitis b viral core-antigen with the pre-core-sequence controlled by the early-late promoter of the 7.5' k protein gene were constructed. the recombinant strains of vaccinia virus were obtained on their basis (vhbe42-1 and vhbe42-3) selectively expressing hbeag of hepatitis b virus. the kinetics of hbeag synthesis was studied in infected cells as well as secretion of the protein into culturing medium. three proteins were found by blotting techn ... | 1991 | 1745274 |
| protective immunity against bovine leukaemia virus (blv) induced in carrier sheep by inoculation with a vaccinia virus-blv env recombinant: association with cell-mediated immunity. | the effects of vaccination of sheep with a recombinant vaccinia virus (rvv) expressing the bovine leukaemia virus (blv) envelope glycoprotein (gp60) were studied by determining blv titres in peripheral blood leukocytes after vaccination and challenge. the proliferation of blv was suppressed markedly, not only when rvv was inoculated prior to challenge with blv, but also when it was inoculated after challenge. these results indicate that vaccination with rvv induces protective immunity that can s ... | 1991 | 1651982 |
| metabolic activation of 4-ipomeanol by complementary dna-expressed human cytochromes p-450: evidence for species-specific metabolism. | 4-ipomeanol is a pulmonary toxin in cattle and rodents that is metabolically activated by cytochromes p-450 (p-450s). p-450-mediated activation of 4-ipomeanol to dna binding metabolites was evaluated using a vaccinia virus complementary dna expression system and an in situ dna-binding assay. twelve human p-450s and two rodent p-450s were expressed in human hepatoma hep g2 cells and examined for their abilities to metabolically activate this toxin. three forms, designated cyp1a2, cyp3a3, and cyp3 ... | 1991 | 1651809 |
| recombinant vaccinia virus expression of the bovine leukaemia virus envelope gene and protection of immunized sheep against infection. | the bovine leukaemia virus (blv) envelope gene encoding extracellular glycoprotein gp51 and transmembrane glycoprotein gp30 was cloned into the ha locus of vaccinia virus (copenhagen strain), downstream of the vaccinia virus early-late promoter, h6, or a triple promoter element consisting of the promoter for the vaccinia virus h6 gene, the promoter for the cowpox virus a-type inclusion (ati) gene and the promoter for the vaccinia virus ha gene. inoculation of rabbits or sheep with the recombinan ... | 1991 | 1645899 |
| nucleotide sequence of 42 kbp of vaccinia virus strain wr from near the right inverted terminal repeat. | the nucleotide sequence of 42090 bp of vaccinia virus strain wr is presented. the sequence includes the sali l, f, g and i fragments and starts near the centre of the hindiii a fragment and extends rightwards towards the genomic terminus, finishing approximately 0.5 kb internal of the inverted terminal repeat (itr). translation of this region has identified 65 open reading frames (orfs) of greater than 65 amino acids in length. fifty-one of these which do not extensively overlap other larger orf ... | 1991 | 2045793 |
| immunological and virological characterization of improved construction of recombinant vaccinia virus expressing rinderpest virus hemagglutinin. | we constructed a recombinant vaccinia virus (rvv) expressing rinderpest virus (rpv) hemagglutinin (h) by modifying the promoter region of the original rvv. the promotor region was modified at three points, i.e., an outframe atg was eliminated, the sequence between the promoter and initiation codon was shortened and the base sequence just upstream of the initiation codon was changed. as compared with the original rvv, the modified rvv was found to produce a remarkably large amount of h protein in ... | 1991 | 2001178 |
| amino acid sequence determination of vaccinia virus immunodominant protein p35 and identification of the gene. | a major immunodominant envelope protein of vaccinia virus (protein p35) was purified by extraction from virions with the nonionic detergent nonidet p-40. the protein was cleaved with cyanogen bromide. four homogeneous peptides were isolated and their n-terminal amino acid sequences determined. a computer search of a protein-sequence data bank revealed complete identity of the determined sequences with sequences 44-63, 144-149, 154-165, and 224-238 of orf h3 of the hindiii-h fragment of the vacci ... | 1991 | 1772969 |
| location of the active site for enzyme-adenylate formation in dna ligases. | the enzyme-amp reaction intermediate of the 102-kda bovine dna ligase i was digested with trypsin, and the adenylylated peptide was isolated by chromatography under conditions that maintain the acid-labile phosphoramidate bond. microsequencing of the peptide showed that it contains an internal trypsin-resistant lysine residue, as expected for the site of adenylylation. inhibition of dna ligase i activity by pyridoxal 5'-phosphate also indicated the presence of a reactive lysine residue in the ca ... | 1991 | 1988940 |
| polylactosaminoglycan modification of the respiratory syncytial virus small hydrophobic (sh) protein: a conserved feature among human and bovine respiratory syncytial viruses. | we investigated the nature of the oligosaccharide modification of the glycosylated forms of the small hydrophobic integral membrane protein, sh (previously designated 1a), of respiratory syncytial (rs) virus. analysis of sh protein expressed in cells infected with rs virus or with a recombinant vaccinia virus revealed two glycosylated sh protein species, shg and shp, which contained n-linked carbohydrate residues. shp migrated diffusely on polyacrylamide gels, which suggested modification by pol ... | 1992 | 1413513 |
| cloning, sequencing, expression, and gq-independent activation of phospholipase c-beta 2. | cdnas corresponding to a previously uncharacterized phospholipase c were isolated from an hl-60 cell cdna library. the cdnas encodes a putative polypeptide of 1181 amino acids with a calculated molecular mass of 133,700 daltons. comparison of the amino acid sequence of the predicted protein with those of five mammalian phospholipase c isoforms (plc-beta 1, plc-gamma 1, plc-gamma 2, plc-delta 1, and plc-delta 2) revealed that the new enzyme is most closely related to plc-beta 1 with an overall am ... | 1992 | 1644792 |
| bovine respiratory syncytial virus nucleocapsid protein: mrna sequence analysis and expression from recombinant vaccinia virus vectors. | the nucleotide sequence of the mrna encoding the nucleocapsid (n) protein of bovine respiratory syncytial (brs) virus, strain 391-2, was determined. recombinant vectors containing a cdna of the complete n gene were constructed, and expression of the n protein in eukaryotic cells was demonstrated using two different vector systems. the brs virus n mrna was 1197 nucleotides in length, exclusive of poly(a), and had a single major open reading frame that encoded a polypeptide of 391 amino acids with ... | 1992 | 1634882 |
| influenza virus hemagglutinin with multibasic cleavage site is activated by furin, a subtilisin-like endoprotease. | many viruses have membrane glycoproteins that are activated at cleavage sites containing multiple arginine and lysine residues by cellular proteases so far not identified. the proteases responsible for cleavage of the hemagglutinin of fowl plague virus, a prototype of these glycoproteins, has now been isolated from madin-darby bovine kidney cells. the enzyme has a mol. wt of 85,000, a ph optimum ranging from 6.5 to 7.5, is calcium dependent and recognizes the consensus sequence r-x-k/r-r at the ... | 1992 | 1628614 |
| in vivo recognition of orf virus early transcriptional promoters in a vaccinia virus recombinant. | the 4.4-kb bamhi-e fragment of the orf virus (ov) genome contains three discrete open reading frames designated orf-pp, orf-1, and orf-3, all of which are flanked by vaccinia virus-like early transcriptional control sequences. to determine whether the vaccinia transcriptional machinery would recognize these promoters and faithfully transcribe ov genes in vivo the bamhi-e fragment was inserted into the thymidine kinase (tk) locus of vaccinia virus and the recombinant used in transcription studies ... | 1992 | 1546449 |
| purification and characterization of dna ligase i from the trypanosomatid crithidia fasciculata. | a dna ligase has been purified approximately 5000-fold, to near homogeneity, from the trypanosomatid crithidia fasciculata. the purified enzyme contains polypeptides with molecular masses of 84 and 80 kda as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. both polypeptides formed enzyme-adenylate complexes in the absence of dna, contained an epitope that is highly conserved between human and bovine dna ligase i and yeast and vaccinia virus dna ligases, and were identified ... | 1992 | 1508676 |
| expression of authentic vaccinia virus-specific and inserted viral and cellular genes under control of an early vaccinia virus promoter is regulated post-transcriptionally in interferon-treated chick embryo fibroblasts. | the interferon sensitivity of the expression of an influenza-virus hemagglutinin (ha) gene cloned into the thymidine kinase (tk) gene of vaccinia virus was studied in chick embryo fibroblasts (cef) and madin-darby bovine kidney (mdbk) cells. in cef, the expression of the ha gene is inhibited by pretreatment of cells with homologous interferon. in mdbk cells, on the other hand, expression of the ha is not impaired by pretreatment with human interferon-alpha, and the synthesis of early vaccinia vi ... | 1992 | 1373550 |
| augmentation of bovine leukemia virus (blv)-specific lymphocyte proliferation responses in ruminants by inoculation with blv env-recombinant vaccinia virus: their role in the suppression of blv replication. | lymphocyte proliferation responses were investigated in sheep and cattle, in which the replication of bovine leukemia virus (blv) had been known to be suppressed by inoculation with recombinant vaccinia virus (rvv) expressing blv envelope glycoprotein (gp60). enhanced lymphocyte proliferation responses were observed in animals inoculated with rvv, regardless of whether they were naive or blv carriers. these responses were roughly inversely correlated to the growth of blv in the peripheral blood ... | 1992 | 1337572 |
| fusogenic segments of bovine leukemia virus and simian immunodeficiency virus are interchangeable and mediate fusion by means of oblique insertion in the lipid bilayer of their target cells. | modified bovine leukemia virus (blv) glycoproteins were expressed by using vaccinia virus recombinants, and their fusogenic capacities were examined by a syncytia-formation assay. this analysis indicates that (i) both blv envelope glycoproteins gp51 and gp30 are necessary for cell fusion; (ii) insertion of the n-terminal segment of gp30 (fusion peptide) into the lipid bilayer in an oblique orientation, as predicted by computer conformational analysis, results in fusogenic capacities higher than ... | 1992 | 1315040 |
| [virucidal effectiveness of some commercial products for chemothermal disinfection methods for temperature resistant viruses and bacteriophages--evaluation of a test model]. | in the laboratory assay of chemothermal virus disinfection procedures a test system is required which allows to differentiate between the physical effects of the temperature and the chemical effects by the disinfectant used. from the four test viruses recommended by the german association for the control of virus diseases (deutsche vereinigung zur bekämpfung der viruskrankheiten) only sv 40 tumor virus showed a sufficient thermal stability at 55 degrees c to take it in consideration as test viru ... | 1992 | 1313238 |
| t-cell responses to highly conserved cd4 and cd8 epitopes on the outer membrane protein of bovine leukemia virus: relevance to vaccine development. | bovine leukemia virus (blv) is a retrovirus that infects cattle and sheep and may provide a model for studying human leukemia. cell-mediated immune mechanisms may play a major role in protection against blv infection. we describe here for the first time the identification of proliferative (cd4) and cytotoxic t-lymphocyte (cd8) epitopes of the gp51 envelope (env) protein of blv. this protein and a recombinant form expressed by a vaccinia virus construct have been shown to be potential vaccine can ... | 1993 | 7680386 |
| single capripoxvirus recombinant vaccine for the protection of cattle against rinderpest and lumpy skin disease. | a recombinant capripoxvirus has been constructed containing a full-length cdna of the fusion protein gene of rinderpest virus. the gene was inserted in the thymidine kinase gene of the capripox genome under the control of the vaccinia virus major late promoter p11 together with the escherichia coli gpt gene in the opposite orientation under the control of the vaccinia early/late promoter p7.5. a vaccine prepared from this recombinant virus protected cattle against clinical rinderpest after a let ... | 1993 | 8342321 |
| role of n-linked glycans in antigenicity, processing, and cell surface expression of bovine herpesvirus 1 glycoprotein giv. | glycoprotein giv, a structural component of bovine herpesvirus type 1, stimulates high titers of virus-neutralizing antibody. the protein contains three potential sites for the addition of n-linked carbohydrates. three mutants were constructed by oligonucleotide-directed mutagenesis, in each case changing one n-linked glycosylation site from asn-x-thr/ser to ser-x-thr/ser. a fourth mutant was altered at two sites. the altered forms of the giv gene were cloned into a vaccinia virus transfer vecto ... | 1993 | 8380463 |
| protection of sheep against bovine leukemia virus (blv) infection by vaccination with recombinant vaccinia viruses expressing blv envelope glycoproteins: correlation of protection with cd4 t-cell response to gp51 peptide 51-70. | we have previously constructed vaccinia virus (vv) recombinants containing a complete or truncated envelope (env) gene of bovine leukemia virus (blv). only recombinants carrying the complete env gene (vv-blv2 and vv-blv3) expressed env glycoprotein on the surface of virus-infected cells and produced an antibody response in rabbits. in the present study, these vv recombinants were used to immunize sheep prior to challenge with blv-infected peripheral blood mononuclear cells. both humoral and cell ... | 1993 | 7680387 |
| priming with recombinant influenza virus followed by administration of recombinant vaccinia virus induces cd8+ t-cell-mediated protective immunity against malaria. | live vectors expressing foreign antigens have been used to induce immunity against several pathogens. however, for the virulent rodent malaria parasite plasmodium yoelii, the use of recombinant vaccinia virus, pseudorabies virus, or salmonella, expressing the circumsporozoite protein of this parasite, failed to induce protection. we generated a recombinant influenza virus expressing an epitope from the circumsporozoite protein of p. yoelii known to be recognized by cd8+ t cells and demonstrated ... | 1993 | 7685119 |
| role of interferons in infectious diseases in the bovine species: effect on viruses and rickettsias. | successful protection was obtained with interferon treatment in experimental viral infections in the bovine species in a number of cases. the efficacy of the treatment against vaccinia virus infection and against rotavirus infection have been demonstrated. on the contrary, bovine herpes virus 1 (bhv 1-causing rhinotracheitis and part of the shipping fever complex) infections were not inhibited by interferon (ifn). the authors have undertaken a study in cattle in zimbabwe to assess the role of in ... | 1993 | 7510899 |
| analysis of bovine herpesvirus 1 glycoprotein giv truncations and deletions expressed by recombinant vaccinia viruses. | glycoprotein giv is an envelope component of bovine herpesvirus type 1 and appears to be involved in attachment, penetration, and cell fusion. four antigenic domains which include both continuous and discontinuous epitopes have been previously defined by competition binding assays using giv-specific monoclonal antibodies (mabs). here we describe the construction of c-terminal truncations and internal deletions in the giv-encoding gene and analyses of the effects of these mutations on the synthes ... | 1993 | 8383232 |
| synthesis and assembly of infectious bovine papillomavirus particles in vitro. | bovine papillomavirus type 1 (bpv-1) virions were produced in vitro using vaccinia virus (vv) recombinants expressing the bpv-1 l1 and l2 capsid proteins. particles morphologically resembling papillomaviruses were observed in the nucleus of cells infected with a vv recombinant for the bpv-1 l1 protein, and greater numbers of similar particles were seen in the nuclei of cells infected with a vv double recombinant for l1 and l2. virus-like particles (vlps) assembled in cells infected with the vv d ... | 1993 | 8385700 |
| immunological characterization of the feline herpesvirus-1 glycoprotein b and analysis of its deduced amino acid sequence. | feline herpesvirus 1 (fhv-1) is an important viral pathogen of cats. like other alphaherpesviruses, fhv-1 contains a hsv-1 glycoprotein b (gb) homolog. in this study, monospecific antisera to hsv-1 gb reacted with three fhv-1 proteins (100, 64, and 58 kda) present in virion lysates by immunoprecipitation and immunoblot analyses. reduced stringency hybridization experiments using a hsv-1 gb probe localized the fhv-1 gb gene to a 9.6-kb sa/l fragment in the unique long region of the genome. northe ... | 1993 | 8212548 |
| identification of two human rho gdp dissociation inhibitor proteins whose overexpression leads to disruption of the actin cytoskeleton. | proteins (ief's 1120, 8118, 8120) sharing similarity to the bovine rho gdp dissociation inhibitor (gdi) have been identified in the human two-dimensional-gel database of keratinocyte proteins. molecular cloning of the corresponding cdnas showed that ief 8118 is the human homolog of bovine gdi while ief 8120 is a distinct although related protein. all available information indicates the ief 1120 is a derivative of ief 8120. the cdnas coding for ief's 8118 and 8120 were recombined into vaccinia vi ... | 1993 | 8262133 |
| processing of the envelope glycoproteins of pestiviruses. | the genomic rna of pestiviruses is translated into a large polyprotein that is cleaved into a number of proteins. the structural proteins are n terminal in this polyprotein and include three glycoproteins called e0, e1, and e2 on the basis of the order in which they appear in the polyprotein. using pulse-chase experiments, we show that a pestiviral glycoprotein precursor, e012, is formed that is processed into e0, e1, and e2 in an ordered fashion. processing is initiated by a nascent cleavage be ... | 1993 | 8388499 |
| recombinant viral vaccines for enzootic bovine leucosis. | recently published studies on the development and use of recombinant vaccinia virus (vv) vaccines incorporating either the complete envelope (env) gene or only a fragment of the env gene consisting of the coding sequence for the env glycoprotein 51 (gp51) and part of gp30 of the bovine leukaemia virus (blv) are described. it has been reported that vaccination of sheep with recombinant vv vaccines containing the complete env gene appears to protect sheep against challenge infection with blv. the ... | 1993 | 8270269 |
| an immunodominant cytotoxic t cell epitope on the vp7 rotavirus protein overlaps the h2 signal peptide. | c57bl/6 (h-2b) mice were primed with the bovine rf strain of rotavirus to study the induction of cd8+ cytotoxic t lymphocytes (ctls). these rotavirus-specific ctls were detected only after in vitro restimulation with the virus. using a recombinant vaccinia virus we identified the rf vp7 protein as a major target of these ctls. the response against this protein was obtained also after in vitro restimulation with simian sa11 and human wa strains of rotavirus. using published db and kb allele-speci ... | 1993 | 8277264 |
| protection of cattle from bhv-1 infection by immunization with recombinant glycoprotein giv. | high levels of recombinant bovine herpesvirus-1 (bhv-1) glycoprotein iv were produced in baculovirus, adenovirus, vaccinia virus and escherichia coli expression systems. the different recombinant forms as well as authentic giv were injected intramuscularly into seronegative calves. with the exception of e. coli-produced giv, all forms of giv induced high levels of neutralizing antibodies both in the serum and in the nasal superficial mucosa. animals immunized with giv produced in insect or mamma ... | 1993 | 8381248 |
| identification of different target glycoproteins for bovine herpes virus type 1-specific cytotoxic t lymphocytes depending on the method of in vitro stimulation. | vaccinia virus recombinants expressing the three major bovine herpes virus-1 (bhv-1) glycoproteins gi, giii and giv were used to identify the major target antigens for bhv-1-specific ctl isolated from immune cattle. peripheral blood mononuclear cells (pbmc) expanded in vitro in the presence of interleukin-2 (il-2) and lysed both giii- and giv-infected target cells. secondary in vitro stimulation of pbmc was also performed in the presence of either fixed bhv-1-infected autologous fibroblasts or u ... | 1993 | 8382189 |
| immunological responses of mice and cattle to baculovirus-expressed f and h proteins of rinderpest virus: lack of protection in the presence of neutralizing antibody. | rinderpest is a highly contagious viral disease of ruminants and has greater than 95% morbidity and mortality. the etiological agent, rinderpest virus (rpv), is a member of the family paramyxoviridae and the genus morbillivirus. immune responses to both the hemagglutinin (h) and the fusion (f) antigens of morbilliviruses play an important role in the prevention of infection, and only attenuated live vaccines have been shown to provide protective immunity against the group. the lack of protection ... | 1993 | 8437215 |
| involvement of cd8+ t cells in delayed-type hypersensitivity responses against bovine leukemia virus (blv) induced in sheep vaccinated with recombinant vaccinia virus expressing blv envelope glycoprotein. | delayed-type hypersensitivity responses against bovine leukemia virus (blv) envelope glycoprotein (gp60) were induced in the skin of sheep vaccinated with recombinant vaccinia virus (rvv) expressing blv glycoprotein. the lesions were characterized by marked infiltration of lymphocytes, slight migration of neutrophils, eosinophils, and macrophages in the dermis to hypodermis, and partial intercellular edema in the reticular layer. immunohistochemical analysis with monoclonal antibodies demonstrat ... | 1993 | 8470333 |
| immunisation of cattle with a recombinant vaccinia vector expressing the haemagglutinin gene of rinderpest virus. | the efficacy of a recombinant rinderpest vaccine, constructed by inserting the rinderpest virus haemagglutinin gene into attenuated vaccinia virus, lc16mo strain, was tested in cattle. after subcutaneous inoculation of 10(8) plaque-forming units (pfu) of the recombinant vaccine, neither palpable skin lesions nor increases in body temperature were observed, indicating the absence of detectable clinical reactions. all the vaccinated cattle were completely protected from challenge with the saudi 1/ ... | 1993 | 8456545 |
| the human keratinocyte two-dimensional protein database (update 1994): towards an integrated approach to the study of cell proliferation, differentiation and skin diseases. | the master two-dimensional (2-d) gel database of human keratinocytes currently lists 3087 cellular proteins (2168 isoelectric focusing, ief; and 919 none-quilibrium ph gradient electrophoresis, nephge), many of which correspond to posttranslational modifications, 890 polypeptides have been identified (protein name, organelle components, etc.) using one or a combination of procedures that include (i) comigration with known human proteins, (ii) 2-d gel immunoblotting using specific antibodies (iii ... | 1994 | 7895731 |
| the gtpase rab3a negatively controls calcium-dependent exocytosis in neuroendocrine cells. | there is accumulating evidence that small gtpases of the rab family regulate intracellular vesicle traffic along biosynthetic and endocytotic pathways in eukaryotic cells. it has been suggested that rab3a, which is associated with synaptic vesicles in neurons and with secretory granules in adrenal chromaffin cells, might regulate exocytosis. we report here that overexpression in pc12 cells of rab3a mutant proteins defective in either gtp hydrolysis or in guanine nucleotide binding inhibited exoc ... | 1994 | 8187757 |
| retrovirus-like particles produced by vaccinia viruses expressing gag-pro-pol region genes of bovine leukaemia virus. | processing and assembly of bovine leukaemia virus-like particles were studied in african green monkey kidney cells using recombinant vaccinia viruses (rvvs) expressing regions of the bovine leukaemia virus genome. unprocessed gag precursor protein (pr44) was detected in immunoblot analysis of lysed cells and particles sedimented from culture supernatants after infection with a rvv carrying the gag and truncated protease (pro) gene. processing of pr44 was observed after infection of cells with a ... | 1994 | 8077921 |