Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| ufd1l, a developmentally expressed ubiquitination gene, is deleted in catch 22 syndrome. | the catch 22 acronym outlines the main clinical features of 22q11.2 deletions (cardiac defects, abnormal facies, thymic hypoplasia, cleft palate and hypocalcemia), usually found in digeorge (dgs) and velo-cardio-facial (vcfs) syndromes. hemizygosity of this region may also be the cause of over 100 different clinical signs. the catch 22 locus maps within a 1.5 mb region, which encompasses several genes. however, no single defect in 22q11.2 hemizygous patients can be ascribed to any gene so far is ... | 1997 | 9063746 |
| cloning and characterization of hupf1, a human homolog of the saccharomyces cerevisiae nonsense mrna-reducing upf1 protein. | levels of most nonsense mrnas are normally reduced in prokaryotes and eukaryotes when compared with that of corresponding functional mrnas. genes encoding polypeptides that selectively reduce levels of nonsense mrna have so far only been identified in simple eukaryotes. we have now cloned a human cdna whose deduced amino acid sequence shows the highest degree of homology to that of upf1, a bona fide saccharomyces cerevisiae group i rna helicase required for accelerated degradation of nonsense mr ... | 1997 | 9064659 |
| proviral integrations at the evi5 locus disrupt a novel 90 kda protein with homology to the tre2 oncogene and cell-cycle regulatory proteins. | evi5 is a common site of retroviral integration in t-cell lymphomas of akxd mice. mapping studies have localized evi5 to a region approximately 18 kb upstream of another common viral integration site, gfi1, on mouse chromosome 5 (liao x, jenkins na and copeland ng, (1995a). j. virol., 69, 7132-7137). gfi1 encodes a zinc finger transcription factor involved in interleukin-2 signaling. to determine if evi5 encodes a gene separate from gfi1 that might also be involved in t-cell disease, we have sea ... | 1997 | 9070650 |
| the s3a ribosomal protein gene is identical to the fte-1 (v-fos transformation effector) gene and the tnf-alpha-induced tu-11 gene, and its transcript level is altered in transformed and tumor cells. | previous work on mouse x rat hybrid cells (bs series) led to the assignment of a transformation suppressor locus (sail) to the rat 5q22-q33 region. this gene is not yet identified. from a non-transformed bs hybrid cell line, we isolated a partial cdna insert (13t), which detects a transcript more abundant in transformed cells than in their non-transformed homologs. sequence comparisons led us to conclude that 13t is identical to the coding sequences of the ribosomal protein s3a gene (rps3a), of ... | 1997 | 9074506 |
| estrogenic activity of a dieldrin/toxaphene mixture in the mouse uterus, mcf-7 human breast cancer cells, and yeast-based estrogen receptor assays: no apparent synergism. | the estrogenic activity of dieldrin, toxaphene, and an equimolar mixture of both compounds (dieldrin/toxaphene) was investigated in the 21-day-old b6c3f1 mouse uterus, mcf-7 human breast cancer cells, and in yeast-based reporter gene assays. treatment of the animals with 17beta-estradiol (e2) (0.0053 kg/day x3) resulted in a 3.1-, 4.8-, and 7.8-fold increase in uterine wet weight, peroxidase activity, and progesterone receptor binding, respectively. in contrast, treatment with 2.5, 15 and 60 mic ... | 1997 | 9075711 |
| molecular cloning of mouse liver flavin containing monooxygenase (fmo1) cdna and characterization of the expression product: metabolism of the neurotoxin, 1,2,3,4-tetrahydroisoquinoline (tiq). | a mouse liver cdna clone, mfmo1, coding for a flavin-containing monooxygenase (fmo) was isolated. this cdna clone encoded a protein of 532 amino acids. based upon its predicted amino acid sequence, this clone was assumed to belong to the fmo1 subfamily. the deduced amino acid sequence showed 94, 84, 83, and 83% identity with fmo1s of rats, pigs, rabbits and humans, respectively, while it showed only 50-59% identity with human fmo3 and fmo4, rabbit fmo2, fmo3, fmo4 and fmo5, and guinea-pig fmo2. ... | 1997 | 9076656 |
| the molecular interaction of fas and fap-1. a tripeptide blocker of human fas interaction with fap-1 promotes fas-induced apoptosis. | fas (apo-1/cd95), which is a member of the tumor necrosis factor receptor superfamily, is a cell surface receptor that induces apoptosis. a protein tyrosine phosphatase, fas-associated phosphatase-1 (fap-1), that was previously identified as a fas binding protein interacts with the c-terminal 15 amino acids of the regulatory domain of the fas receptor. to identify the minimal region of the fas c-terminal necessary for binding to fap-1, we employed an in vitro inhibition assay of fas/fap-1 bindin ... | 1997 | 9079683 |
| isolation and characterization of cdna encoding mouse rna polymerase ii subunit rpb14. | by means of the yeast two-hybrid system using the 40-kda subunit of mouse rna polymerase i, mrpa40, as the bait, we isolated a mouse cdna which encoded a protein with significant homology in amino acid sequence to the 12.5-kda subunit of saccharomyces cerevisiae rna polymerase ii, b12.5 (rpb11). specific antibody raised against the recombinant protein that was derived from the cdna reacted with a 14-kda polypeptide in highly purified mammalian rna polymerase ii and did not react with any subunit ... | 1997 | 9099876 |
| functional analysis of the fission yeast prp4 protein kinase involved in pre-mrna splicing and isolation of a putative mammalian homologue. | the prp4 gene of schizosaccharomyces pombe encodes a protein kinase. a physiological substrate is not yet known. a mutational analysis of prp4 revealed that the protein consists of a short n-terminal domain, containing several essential motifs, which is followed by the kinase catalytic domain comprising the c-terminus of the protein. overexpression of n-terminal mutations disturbs mitosis and produces elongated cells, using a pcr approach, we isolated a putative homologue of prp4 from human and ... | 1997 | 9102632 |
| molecular evolution of cytochrome c oxidase subunit iv: evidence for positive selection in simian primates. | cytochrome c oxidase (cox) is a multi-subunit enzyme complex that catalyzes the final step of electron transfer through the respiratory chain on the mitochondrial inner membrane. up to 13 subunits encoded by both the mitochondrial (subunits i, ii, and iii) and nuclear genomes occur in eukaryotic organisms ranging from yeast to human. previously, we observed a high number of amino acid replacements in the human cox iv subunit compared to mouse, rat, and cow orthologues. here we examined cox iv ev ... | 1997 | 9115172 |
| regulation of the myeloid-cell-expressed human gp91-phox gene as studied by transfer of yeast artificial chromosome clones into embryonic stem cells: suppression of a variegated cellular pattern of expression requires a full complement of distant cis elements. | identifying the full repertoire of cis elements required for gene expression in mammalian cells (or animals) is challenging, given the moderate sizes of many loci. to study how the human gp91-phox gene is expressed specifically in myeloid hematopoietic cells, we introduced yeast artificial chromosome (yac) clones and derivatives generated in yeast into mouse embryonic stem cells competent to differentiate to myeloid cells in vitro or into mouse chimeras. fully appropriate regulation was recapitu ... | 1997 | 9121478 |
| stat5 is a physiological substrate of the insulin receptor. | using the cytoplasmic domain of the insulin receptor (ir) in a yeast two-hybrid screen, we identified a cdna clone encoding the c-terminal 308 amino acids of human stat5b (stat5b-ct). stat5b-ct is tyrosine phosphorylated by purified ir kinase domain in vitro. insulin stimulates tyrosine phosphorylation of overexpressed stat5b-ct and endogenous stat5 in cells overexpressing ir. stat5 may be a direct target of the ir and, as a member of the stat family of transcription factors, may play a role in ... | 1997 | 9122188 |
| binary transgenic mouse model for studying the trans control of globin gene switching: evidence that gata-1 is an in vivo repressor of human epsilon gene expression. | to test whether human gata-1 (hgata-1) is involved in the transcriptional control of globin gene switching, we produced transgenic mice overexpressing hgata-1, crossed them with mice carrying a human beta-globin locus yeast artificial chromosome (beta yac), and analyzed globin gene expression in their progeny. mice carrying both the hgata-1 and the beta yac transgenes had normal levels of gamma- and beta-globin mrna and no distortion in the rate or in the timing of gamma-to-beta switch, indicati ... | 1997 | 9122214 |
| harakiri, a novel regulator of cell death, encodes a protein that activates apoptosis and interacts selectively with survival-promoting proteins bcl-2 and bcl-x(l). | programmed cell death is essential in organ development and tissue homeostasis and its deregulation is associated with the development of several diseases in mice and humans. the precise mechanisms that control cell death have not been elucidated fully, but it is well established that this form of cellular demise is regulated by a genetic program which is activated in the dying cell. here we report the identification, cloning and characterization of harakiri, a novel gene that regulates apoptosi ... | 1997 | 9130713 |
| a mutation in the xpb/ercc3 dna repair transcription gene, associated with trichothiodystrophy. | trichothiodystrophy (ttd) is a rare, autosomal recessive disorder characterized by sulfur-deficient brittle hair and nails, mental retardation, impaired sexual development, and ichthyosis. photosensitivity has been reported in approximately 50% of the cases, but no skin cancer is associated with ttd. virtually all photosensitive ttd patients have a deficiency in the nucleotide excision repair (ner) of uv-induced dna damage that is indistinguishable from that of xeroderma pigmentosum (xp) complem ... | 1997 | 9012405 |
| structure and expression of the mouse casein gene locus. | the analysis of yeast artificial chromosomes (yacs) containing the complete mouse casein gene locus revealed the presence of five casein genes, alpha-, beta-, gamma-, delta-, and kappa-casein, in this order, in the locus. the alpha- and beta-casein genes are only 10 kb apart and have convergent transcriptional orientations. the distance between the beta-casein gene and the alpha s2-like gamma-casein gene is about 70 kb, and these genes have divergent transcriptional orientations. the gamma- and ... | 1997 | 9021141 |
| investigation of growth hormone releasing hormone receptor structure and activity using yeast expression technologies. | growth hormone releasing hormone (ghrh) is the positive regulator of growth hormone synthesis and secretion in the anterior pituitary. the peptide confers activity by binding to a seven transmembrane domain g protein-coupled receptor. signal transduction proceeds through subsequent g alpha s stimulation of adenylyl cyclase. to investigate ligand/receptor and receptor/g protein associations, the human ghrh receptor was expressed in a modified s. cerevisiae strain which allows for facile measureme ... | 1997 | 9029497 |
| the multiple murine 3 beta-hydroxysteroid dehydrogenase isoforms: structure, function, and tissue- and developmentally specific expression. | the enzyme 3 beta-hydroxysteroid dehydrogenase (3 beta-hsd) is essential for the biosynthesis of all active steroid hormones. to date five distinct isoforms have been identified in the mouse. the different isoforms are indicated by roman numerals (i-v) in the chronological order in which they have been isolated. the different isoforms are expressed in a tissue- and developmentally specific manner and fall into two functionally distinct groups. 3 beta-hsd i, ii, and iii function as nad(+)-depende ... | 1997 | 9029733 |
| incubation at the nonpermissive temperature induces deficiencies in uv resistance and mutagenesis in mouse mutant cells expressing a temperature-sensitive ubiquitin-activating enzyme (e1). | in temperature-sensitive (ts) mutants of mouse fm3a cells, the levels of mutagenesis and survival of cells treated with dna-damaging agents have been difficult to assess because they are killed after their mutant phenotypes are expressed at the nonpermissive temperature. to avoid this difficulty, we incubated the ts mutant cells at the restrictive temperature, 39 degrees c, for only a limited period after inducing dna damage. we used ts mutants defective in genes for ubiquitin-activating enzyme ... | 1997 | 9032276 |
| mitochondrial dna polymerases from yeast to man: a new family of polymerases. | we report the sequence of a 4.5-kb cdna clone isolated from a human melanoma library which bears high amino acid sequence identity to the yeast mitochondrial (mt) dna polymerase (mip1p). this cdna contains a 3720-bp open reading frame encoding a predicted 140-kda polypeptide that is 43% identical to mip1p. the n-terminal part of the sequence contains a 13 glutamine stretch encoded by a cag trinucleotide repeat which is not found in the other dna polymerases gamma (pol gamma). multiple amino acid ... | 1997 | 9034326 |
| lethal effect of recombinant human fas ligand in mice pretreated with propionibacterium acnes. | fas ligand (fasl) is a type ii membrane protein. binding of fasl to its receptor, fas, induces apoptosis. matrix metalloproteinase cleaves the membrane-bound human fasl to yield the active soluble form. here, we have produced a large amount of human soluble rfasl using the yeast, pichia pastoris. the purified rfasl was found to be glycosylated and to exist as a trimer. the rfasl was effective in inducing apoptosis in a fas-expressing t cell or a fibroblast cell line. the id50 of rfasl for mouse ... | 1997 | 9036978 |
| characterization of two age-induced intracisternal a-particle-related transcripts in the mouse liver. transcriptional read-through into an open reading frame with similarities to the yeast ccr4 transcription factor. | intracisternal a-particle (iap) sequences are endogenous retrovirus-like elements present at 1,000 copies in the mouse genome. we had previously identified iap-related transcripts of unusual size (6 and 10 kilobases (kb)), which are observed exclusively in the liver of the aging mouse. in this report, using cdna libraries that we have constructed from the liver mrnas of an aged dba/2 mouse, we have cloned and entirely sequenced the corresponding cdnas. both are initiated within the 5' long termi ... | 1997 | 9038221 |
| cloning and structural analysis of the murine gcn5l1 gene. | we recently cloned the murine 11-cis retinol dehydrogenase gene. a second gene, the murine gcn5l1 gene, was found to be situated upstream of the murine 11-cis retinol dehydrogenase gene. we have isolated and sequenced the complete coding sequence of the murine gcn5l1 gene. the distance between the 3'-end of the murine gcn5l1 gene and the 5'-end of the 11-cis retinol dehydrogenase gene is only 776 nt. the murine gcnsl1 gene consists of four exons encompassing approximately 3.5 kb of genomic dna. ... | 1997 | 9426003 |
| genomic organization, cdna sequence, bacterial expression, and purification of human seryl-trna synthase. | in this paper, we report the cdna sequence and deduced primary sequence for human cytosolic seryl-trna synthetase, and its expression in escherichia coli. two human brain cdna clones of different origin, containing overlapping fragments coding for human seryl-trna synthetase were sequenced: hfbdn14 (fetal brain clone); and ib48 (infant brain clone). for both clones the 5' region of the cdna was missing. this 5' region was obtained via pcr methods using a human brain 5' race-ready cdna library. t ... | 1997 | 9431993 |
| molecular cloning of tvdao1, a gene encoding a d-amino acid oxidase from trigonopsis variabilis and its expression in saccharomyces cerevisiae and kluyveromyces lactis. | the dao1 gene of trigonopsis variabilis encoding a d-amino acid oxidase (ec 1.4.3.3) was isolated from genomic clones selected for their specific hybridization to synthetic oligodeoxyribonucleotide probes based on regions of the enzyme that have been conserved through evolution. the nucleotide sequence of the gene predicts a protein with similarities to human, pig, rabbit, mouse and fusarium solani d-amino acid oxidases. the open reading frame of the t. variabilis dao1 gene was interrupted by an ... | 1997 | 9434346 |
| mammalian rad51 protein: a reca homologue with pleiotropic functions. | during the last years, homologues of e coli reca have been cloned in numerous species including man. these rad51 proteins share sequence as well as functional homologies with the bacterial protein. human rad51 (hsrad51) is able to catalyze strand exchange in vitro between homologous dnas, but with a lower efficiency compared to that of reca. this suggests the requirement of additional factors. a very interesting feature of rad51 is its essential role in mouse which could mean that it has gained ... | 1997 | 9466696 |
| human sak related to the plk/polo family of cell cycle kinases shows high mrna expression in testis. | we identified the nucleotide sequence of a cdna encoding a polypeptide with a kinase domain that is related to the catalytic region of drosophila melanogaster polo, saccharomyces cerevisiae cdc5 as well as human fnk and plk. the novel gene seems to represent the human counterpart of the mouse gene sak. the sequence of sak predicts a serine/threonine kinase of 970 aa. the distribution of sak mrna in adult organs is restricted to certain tissues such as testis and thymus. northern analyses of tumo ... | 1997 | 21590086 |
| rodent y chromosome tspy gene is functional in rat and non-functional in mouse. | recombination is believed to prevent genetic deterioration in sexual populations because it allows conservation of functional genotypes by removing deleterious mutations. moreover, evidence that non-recombining segments of a genome deteriorate is provided by genetic experiments in drosophila and yeast. y chromosomes generally do not recombine along most of their length, and thus y chromosome genes, despite having been selectively maintained for their function, could be lost from the genome. here ... | 1998 | 9467017 |
| a new rac target posh is an sh3-containing scaffold protein involved in the jnk and nf-kappab signalling pathways. | the rho, rac and cdc42 gtpases coordinately regulate the organization of the actin cytoskeleton and the jnk map kinase pathway. mutational analysis of rac has previously shown that these two activities are mediated by distinct cellular targets, though their identity is not known. two rac targets, p65(pak) and mlk, are ser/thr kinases that have been reported to be capable of activating the jnk pathway. we present evidence that neither is the rac target mediating jnk activation in cos-1 cells. we ... | 1998 | 9482736 |
| functional comparison of the yeast scerv1 and scerv2 genes. | the yeast scerv1 gene is the first representative of a new emerging gene family. its gene product is essential for the yeast cell and is involved in the biogenesis of mitochondria and the regulation of the cell cycle. recently the general importance of the gene for the eukaryotic cell was shown by the identification of a structural and functional human homologue. the homologous mammalian alr (augmenter of liver regeneration) genes from man, mouse and rat are important for different developmental ... | 1998 | 9483805 |
| complex formation of smap/kap3, a kif3a/b atpase motor-associated protein, with a human chromosome-associated polypeptide. | we have recently isolated smap (smg gds-associated protein; smg gds: small g protein gdp dissociation stimulator) as a novel smg gds-associated protein, which has armadillo repeats and is phosphorylated by src tyrosine kinase. smap is a human counterpart of mouse kap3 (kinesin superfamily-associated protein) that is associated with mouse kif3a/b (a kinesin superfamily protein), which functions as a microtubule-based atpase motor for organelle transport. we isolated here a smap-interacting protei ... | 1998 | 9506951 |
| molecular and biochemical characterisation of dna-dependent protein kinase-defective rodent mutant irs-20. | the catalytic subunit of the dna-dependent protein kinase (dna-pkcs) is a member of a sub-family of phosphatidylinositol (pi) 3-kinases termed pik-related kinases. a distinguishing feature of this sub-family is the presence of a conserved c-terminal region downstream of a pi 3-kinase domain. mutants defective in dna-pkcs are sensitive to ionising radiation and are unable to carry out v(d)j recombination. irs-20 is a dna-pkcs-defective cell line with milder gamma-ray sensitivity than two previous ... | 1998 | 9518490 |
| mutations in either nucleotide-binding site of p-glycoprotein (mdr3) prevent vanadate trapping of nucleotide at both sites. | vanadate trapping of nucleotide and site-directed mutagenesis were used to investigate the role of the two nucleotide-binding (nb) sites in the regulation of atp hydrolysis by p-glycoprotein (mouse mdr3). mdr3, tagged with a hexahistidine tail, was overexpressed in the yeast pichia pastoris and purified to about 90% homogeneity by ni-affinity chromatography. this protocol yielded purified, reconstituted mdr3 which exhibited high verapamil stimulation of atpase activity with a vmax of 4.2 micromo ... | 1998 | 9521779 |
| the largest subunit of mouse rna polymerase ii (rpb1) functionally substituted for its yeast counterpart in vivo. | the full-length mouse rna polymerase ii (pol ii) largest subunit (rpb1) gene was used to replace 5070 bp of the yeast saccharomyces cerevisiae rpb1 gene via homologous recombination and gene replacement in vivo. transcription of the mouse rpb1 gene using the yeast promoter in the haploid state was confirmed by northern analysis. this strain of yeast is viable, indicating that mouse rpb1 is able to interact functionally with the other yeast rna pol ii subunits in vivo. | 1998 | 9524248 |
| cloning of human p55 gamma, a regulatory subunit of phosphatidylinositol 3-kinase, by a yeast two-hybrid library screen with the insulin-like growth factor-i receptor. | we have used the yeast two-hybrid system to identify proteins that interact with the intracellular domain of the insulin-like growth factor-i receptor (igfir). in a search of a human fetal brain library we identified a cdna encoding a protein that is the human homologue of mouse p55pik, a regulatory subunit of phosphatidylinositol 3-kinase (hp55 gamma). the hp55 gamma protein interacts strongly with the activated igfir but not with the kinase-negative mutant receptor. hp55 gamma also interacts w ... | 1998 | 9524259 |
| shc and enigma are both required for mitogenic signaling by ret/ptc2. | ret/ptc2 is a constitutively active, oncogenic form of the c-ret receptor tyrosine kinase. like the other papillary thyroid carcinoma forms of ret, ret/ptc2 is activated through fusion of the ret tyrosine kinase domain to the dimerization domain of another protein. investigation of requirements for ret/ptc2 mitogenic activity, using coexpression with dominant negative forms of ras and raf, indicated that these proteins are required for mitogenic signaling by ret/ptc2. because activation of ras r ... | 1998 | 9528800 |
| the primary and final effector mechanisms required for kinin-induced epithelial chloride secretion. | the short-circuit current technique was used to examine the effects of n2-l-lysylbradykinin (lbk) on chloride secretion in the mucosae of the mouse intestine. it was found to be a potent chloride secretagogue in the mucosa lining the colon, jejunum, and cecum, as it is in most mammals, with 2 nm being sufficient to cause half-maximal secretion. the extent of the responses was in the order cecum > colon > jejunum. in cystic fibrosis (cf) null mice, with no cf transmembrane conductance regulator ( ... | 1998 | 9530160 |
| a homologue of saccharomyces cerevisiae dpm1p is not sufficient for synthesis of dolichol-phosphate-mannose in mammalian cells. | dolichol-phosphate-mannose (dol-p-man) serves as a donor of mannosyl residues in major eukaryotic glycoconjugates. it donates four mannosyl residues in the n-linked oligosaccharide precursor and all three mannosyl residues in the core of the glycosylphosphatidylinositol anchor. in yeasts it also donates one mannose to the o-linked oligosaccharide. the yeast dpm1 gene encodes a dol-p-man synthase that is a transmembrane protein expressed in the endoplasmic reticulum. we cloned human and mouse hom ... | 1998 | 9535917 |
| functional copies of a human gene can be directly isolated by transformation-associated recombination cloning with a small 3' end target sequence. | unique, small sequences (sequence tag sites) have been identified at the 3' ends of most human genes that serve as landmarks in genome mapping. we investigated whether a single copy gene could be isolated directly from total human dna by transformation-associated recombination (tar) cloning in yeast using a short, 3' unique target. a tar cloning vector was constructed that, when linearized, contained a small amount (381 bp) of 3' hypoxanthine phosphoribosyltransferase (hprt) sequence at one end ... | 1998 | 9539761 |
| [molecular analysis of peroxisomal disorders]. | peroxisome biogenesis disorders (pbd) include zellweger syndrome (zs), neonatal adrenoleukodystrophy (nald) and infantile refsum disease (ird). they are classified into ten complementation groups. five pathogenic genes have been identified using different model systems of peroxisome deficient mutants. paf-1 and 2 were identified from cho mutants and were responsible genes for pbd group f and c. human pex 5, 12 and 1, responsible genes for group 2, 3 and 1, respectively, were cloned by homology s ... | 1998 | 9545777 |
| centromere protein b null mice are mitotically and meiotically normal but have lower body and testis weights. | cenp-b is a constitutive centromere dna-binding protein that is conserved in a number of mammalian species and in yeast. despite this conservation, earlier cytological and indirect experimental studies have provided conflicting evidence concerning the role of this protein in mitosis. the requirement of this protein in meiosis has also not previously been described. to resolve these uncertainties, we used targeted disruption of the cenpb gene in mouse to study the functional significance of this ... | 1998 | 9548711 |
| two high conductance channels of the mitochondrial inner membrane are independent of the human mitochondrial genome. | patch-clamp techniques were used to characterize the channel activity of mitochondrial inner membranes of two human osteosarcoma cell lines: a mitochondrial genome-deficient (rho0) line and its corresponding parental (rho+) line. previously, two high conductance channels, mitochondrial centum picosiemen (mcs) and multiple conductance channels (mcc), were detected in murine mitochondria. while mcc was assigned to the protein import in yeast mitochondria, the role of mcs is unknown. this study dem ... | 1998 | 9559661 |
| trichoderma reesei prs12 encodes a stress- and unfolded-protein-response-inducible regulatory subunit of the fungal 26s proteasome. | we have cloned a gene, prs12, from the filamentous fungus trichoderma reesei which encodes a fungal homologue of the mouse and drosophila regulatory subunit 12 of the 26s proteasome (mov34). sequencing of both a genomic and a cdna-clone predicts a 342-aa protein with high overall identity (56-68 %) to the homologous counterparts from human, mammals, drosophila and saccharomyces cerevisiae. the predicted protein contains several consensus sequences for phosphorylation, three of which are conserve ... | 1998 | 9560436 |
| androgen receptor yac transgenic mice carrying cag 45 alleles show trinucleotide repeat instability. | x-linked spinal and bulbar muscular atrophy (sbma) is caused by a cag repeat expansion in the first exon of the androgen receptor (ar) gene. disease-associated alleles (37-66 cags) change in length when transmitted from parents to offspring, with a significantly greater tendency to shift size when inherited paternally. as transgenic mice carrying human ar cdnas with 45 and 66 cag repeats do not display repeat instability, we attempted to model trinucleotide repeat instability by generating trans ... | 1998 | 9580659 |
| a critical role for cyclin c in promotion of the hematopoietic cell cycle by cooperation with c-myc. | cyclin c, a putative g1 cyclin, was originally isolated through its ability to complement a saccharomyces cerevisiae strain lacking the g1 cyclin gene cln1-3. unlike cyclins d1 and e, the other two g1 cyclins obtained by the same approach and subsequently shown to play important roles during the g1/s transition, there is thus far no evidence to support the hypothesis that cyclin c is indeed critical for the promotion of cell cycle progression. in baf-b03 cells, an interleukin 3 (il-3)-dependent ... | 1998 | 9584184 |
| optimal activation of an endogenous gene by hox11 requires the nh2-terminal 50 amino acids. | the hox11 homeobox gene was first identified through studies of the t(7;10) and t(10;14) chromosomal translocations of acute t-cell leukemia. in addition, analysis of hox11-/- mice has demonstrated a critical role for this gene in murine spleen development. a possible mode of in vivo function for the hox11 protein in these two situations is regulation of target genes following dna binding via the homeodomain, but little is known about how hox11 regulates transcription in vivo. by performing tran ... | 1998 | 9584190 |
| interaction of mouse polycomb-group (pc-g) proteins enx1 and enx2 with eed: indication for separate pc-g complexes. | the polycomb group (pc-g) constitutes an important, functionally conserved group of proteins, required to stably maintain inactive homeobox genes repressed during development. drosophila extra sex combs (esc) and its mammalian homolog embryonic ectoderm development (eed) are special pc-g members, in that they are required early during development when pc-g repression is initiated, a process that is still poorly understood. to get insight in the molecular function of eed, we searched for eed-inte ... | 1998 | 9584197 |
| mechanism for benomyl action as a mitochondrial aldehyde dehydrogenase inhibitor in mice. | benomyl (a non-thio fungicide) inhibits hepatic mitochondrial low-km aldehyde dehydrogenase (maldh or aldh2) in ip-treated mice by 50% (ic50) at 7.0 mg/kg, which is surprisingly the same potency range as that for several dithiocarbamate fungicides (and the related alcohol abuse drug disulfiram) and thiocarbamate herbicides previously known for their alcohol-sensitizing action. the mechanism by which benomyl inhibits maldh was therefore examined, first by comparing the metabolism of benomyl with ... | 1998 | 9585485 |
| identification of mouse ulk1, a novel protein kinase structurally related to c. elegans unc-51. | a novel protein kinase related to the c. elegans serine/threonine kinase unc-51 was cloned from mouse. the unc-51-like kinase (ulk)1 is encoded by a cdna of 1051 amino acids with calculated mw of 113 kda. comparison of the ulk1 and unc-51 shows the highest conservation in the amino-terminal kinase domain, which is followed by a proline/serine-rich (ps) domain and a conserved carboxyl-terminal (c) domain. ulk1 mrna is expressed in various tissues, and is mapped to mouse chromosome 5f and rat chro ... | 1998 | 9600096 |
| the lissencephaly gene product lis1, a protein involved in neuronal migration, interacts with a nuclear movement protein, nudc. | important clues to how the mammalian cerebral cortex develops are provided by the analysis of genetic diseases that cause cortical malformations [1-5]. people with miller-dieker syndrome (mds) or isolated lissencephaly sequence (ils) have a hemizygous deletion or mutation in the lis1 gene [3,6]; both conditions are characterized by a smooth cerebral surface, a thickened cortex with four abnormal layers, and misplaced neurons [7,8]. lis1 is highly expressed in the ventricular zone and the cortica ... | 1998 | 9601647 |
| ribonucleases from rat and bovine liver: purification, specificity and structural characterization. | the presence of four members of the pyrimidine-specific ribonuclease superfamily was demonstrated in rat liver. three of them (rl1, rl2 and rl3) were purified and showed ribonuclease activity at ph 7.5 with yeast rna as substrate. rl1 is identical to rat pancreatic ribonuclease (ribonuclease 1). n-terminal sequence analysis showed the presence of the native protein and several n-terminally degraded components. rl2 and rl3 were n-terminally blocked proteins. after acidic cleavage or cnbr digestio ... | 1998 | 9602056 |
| isolation and characterization of a new gene encoding a member of the hira family of proteins from drosophila melanogaster. | the hira family of genes (named after yeast hir genes; hir is an acronym for 'histone regulator') includes the yeast hir1 and hir2 repressors of histone gene transcription in s. cerevisiae, human tuple-1/hira, chicken hira, and mouse hira. here, we describe a new member of the hira family, dhh, for the drosophila homolog of hira . northern analysis with poly (a)+ mrna isolated from different developmental stages of drosophila melanogaster shows hybridization with a single dhh transcript of 4.1kb ... | 1998 | 9611274 |
| rustmicin, a potent antifungal agent, inhibits sphingolipid synthesis at inositol phosphoceramide synthase. | rustmicin is a 14-membered macrolide previously identified as an inhibitor of plant pathogenic fungi by a mechanism that was not defined. we discovered that rustmicin inhibits inositol phosphoceramide synthase, resulting in the accumulation of ceramide and the loss of all of the complex sphingolipids. rustmicin has potent fungicidal activity against clinically important human pathogens that is correlated with its sphingolipid inhibition. it is especially potent against cryptococcus neoformans, w ... | 1998 | 9614099 |
| physical and genetic maps of the deafwaddler region on distal mouse chr 6. | the deafwaddler (dfw) mutation, displaying motor ataxia and profound deafness, arose spontaneously in a c3h/hej colony and was mapped previously to distal mouse chr 6. in this study, a high-resolution genetic map was generated by positioning 10 microsatellite markers and 5 known genes on a 968-meioses intersubspecific backcross segregating for dfw [(cast/ei(-)+/+ x c3heb/ fej-dfw/dfw) x c3heb/fej-dfw/dfw], giving the following marker order and sex-averaged distances: d6mit64-(0.10 + 0.10 cm)-pan ... | 1998 | 9615221 |
| flow cytometric quantitation of yeast a novel technique for use in animal model work and in vitro immunologic assays. | animal models of fungal and other infectious diseases often require that the number of organisms in tissue be quantified, traditionally by grinding organs, plating them on agar and counting colony forming units (cfu). this method is labor intensive, slow as some fungi require two weeks of culture and limited in reliability by poor plating efficiency. to circumvent these problems, we developed a flow cytometric method to quantify yeast. in vitro cultured blastomyces dermatitidis, cryptococcus neo ... | 1998 | 9617831 |
| nucleolar localization of the werner syndrome protein in human cells. | werner syndrome (ws) is a human genetic disorder with many features of premature aging. the gene defective in ws (wrn) has been cloned and encodes a protein homologous to several helicases, including escherichia coli recq, the human bloom syndrome protein (blm), and saccharomyces cerevisiae sgs1p. to better define the function of wrn protein we have determined its subcellular localization. indirect immunofluorescence using polyclonal anti-human wrn shows a predominant nucleolar localization. stu ... | 1998 | 9618508 |
| telomere elongation by hnrnp a1 and a derivative that interacts with telomeric repeats and telomerase. | telomeric dna of mammalian chromosomes consists of several kilobase-pairs of tandemly repeated sequences with a terminal 3' overhang in single-stranded form. maintaining the integrity of these repeats is essential for cell survival; telomere attrition is associated with chromosome instability and cell senescence, whereas stabilization of telomere length correlates with the immortalization of somatic cells. telomere elongation is carried out by telomerase, an rna-dependent dna polymerase which ad ... | 1998 | 9620782 |
| rim2 (recombination-induced mutation 2) is a new allele of pearl and a mouse model of human hermansky-pudlak syndrome (hps): genetic and physical mapping. | a mouse mutation, rim2, is one of a series of spontaneous mutations that arose from the intra-mhc recombinants between japanese wild mouse-derived wm7 and laboratory mhc haplotypes. this mutation is single recessive and characterized by diluted coat color and hypo-pigmentation of the eyes. we mapped the rim2 gene close to an old coat color mutation, pearl (pe), on chromosome (chr) 13 by the high-density linkage analysis. the pearl mutant is known to have abnormalities similar to hermansky-pudlak ... | 1998 | 9434937 |
| molecular evolution of hox gene regulation: cloning and transgenic analysis of the lamprey hoxq8 gene. | the mammalian hox clusters arose by duplication of a primordial cluster. the duplication of hox clusters created redundancy within cognate groups, allowing for change in function over time. the lamprey, petromyzon marinus, occupies an intermediate position within the chordates, both in terms of morphologic complexity and possibly cluster number. to determine the extent of divergence among hox genes after duplication events within vertebrates, we analyzed hox genes belonging to cognate group 8. h ... | 1998 | 9437854 |
| interferon-responsive protein kinase (p68) and proliferating cell nuclear antigen are inversely distributed in head and neck squamous cell carcinoma. | pkr (protein kinase, interferon-responsive) is a ribosomal-associated protein kinase found in all human cells. when activated by dsrna or polyanionic substances, pkr efficiently inhibits cellular protein synthesis. pkr expression has been correlated with cellular differentiation in a number of tumor types, including squamous cell carcinoma of the head and neck region. although transfection of pkr into mouse fibroblasts and yeast cells inhibits proliferation, it is not known if modulation of nati ... | 1998 | 9422082 |
| apolipoprotein(a) yeast artificial chromosome transgenic rabbits. lipoprotein(a) assembly with human and rabbit apolipoprotein b. | the in vivo analysis of lipoprotein(a) (lp(a)), an independent atherosclerosis risk factor in humans, has been limited in part by its restricted distribution among mammals. although transgenic mice have been created containing lp(a), the relatively small size of the mouse has precluded some studies. to examine the properties of this molecule in a significantly larger mammal, we have used a 270-kilobase yeast artificial chromosome clone containing the human apolipoprotein(a) (apo(a)) gene and a 9 ... | 1998 | 9422793 |
| a candidate mammalian dna methyltransferase related to pmt1p of fission yeast. | trace levels of 5-methylcytosine persist in the dna of mouse embryonic stem cells that are homozygous for null mutations in dnmt1 , the gene for the one previously recognized metazoan dna methyltransferase. this residual 5-methylcytosine may be the product of a candidate second dna methyltransferase, dnmt2, that has now been identified in human and mouse. dnmt2 contains all the sequence motifs diagnostic of dna (cytosine-5)-methyltransferases but appears to lack the large n-terminal regulatory d ... | 1998 | 9425235 |
| mutations in prop1 cause familial combined pituitary hormone deficiency. | combined pituitary hormone deficiency (cphd) in man denotes impaired production of growth hormone (gh) and one or more of the other five anterior pituitary hormones. mutations of the pituitary transcription factor gene pou1f1 (the human homologue of mouse pit1) are responsible for deficiencies of gh, prolactin and thyroid stimulating hormone (tsh) in snell and jackson dwarf mice and in man, while the production of adrenocorticotrophic hormone (acth), luteinizing hormone (lh) and follicle stimula ... | 1998 | 9462743 |
| interaction of bp180 (type xvii collagen) and alpha6 integrin is necessary for stabilization of hemidesmosome structure. | the hemidesmosome is a multimolecular complex that integrates the extracellular matrix with the keratin cytoskeleton and that stabilizes epithelial attachment to connective tissue. a 180 kda protein (bp180, type xvii collagen), first identified by its reactivity with autoantibodies in the serum of patients with a blistering skin disease called bullous pemphigoid (bp), is a transmembrane component of the hemidesmosome with a collagen-like extracellular domain. here, using recombinantly expressed ... | 1998 | 9856810 |
| identification of strap, a novel wd domain protein in transforming growth factor-beta signaling. | transforming growth factor-beta1 (tgf-beta1) is the prototype of a large family of proteins that regulate a variety of biological processes. the pleiotropic responses to tgf-beta are mediated via ligand-induced heteromeric complex formation by type i (tbetar-i) and type ii (tbetar-ii) serine-threonine kinase receptors. several studies have shown that tbetar-ii acts as a primary receptor, binding tgf-beta and phosphorylating tbetar-i, whose kinase activity then propagates the signals. therefore, ... | 1998 | 9856985 |
| the mouse genome contains two expressed intronless retroposed pseudogenes for the sentrin/sumo-1/pic1 conjugating enzyme ubc9. | the ubiquitin conjugating (ubc) e2 enzyme ubc-9 conjugates the ubiquitin-like peptide sentrin/sumo-1/pic1 to target proteins which include the fas antigen. we show that the mouse genome contains four copies of the ubc-9 gene. these include a structural ubc-9 gene consisting of seven exons which encode a protein identical to human ubc-9, and three intronless processed pseudogenes. the open reading frames (orf) of two of the pseudogenes, ubc9-psi1 and ubc9-psi2, correspond to the cdna of ubc-9 and ... | 1998 | 10068040 |
| genetic and physical mapping of the lps locus: identification of the toll-4 receptor as a candidate gene in the critical region. | on the basis of 2093 meioses analyzed in two separate intraspecific backcrosses, the location of the mouse lpsd mutation was circumscribed to a genetic interval 0.9 cm in size. a total of 19 genetic markers that lie in close proximity to the mutation were examined in mapping. most of these were previously unpublished polymorphic microsatellites, identified by fragmentation of yac and bac clones spanning the region of interest. lpsd was found to be inseparable from the microsatellite marker d4mit ... | 1998 | 10087992 |
| excision repair of 8-hydroxyguanine in mammalian cells: the mouse ogg1 protein as a model. | 8-hydroxyguanine (8-oh-gua) is a major mutagenic lesion produced on dna by the oxidative stress induced by either the endogen metabolism or the exposure to external agents. in bacteria and yeast this modified base can be removed by specific dna glycosylases. recently a human gene coding for an 8-oh-gua dna glycosylase/ap lyase has been identified by its homology to the yeast ogg1. this gene is located in human chromosome 3p25, a region commonly rearranged in various cancers, specially in lung tu ... | 1998 | 10098454 |
| identification and characterization of stk12/aik2: a human gene related to aurora of drosophila and yeast ipl1. | mutations in aurora of drosophila and related saccharomyces cerevisiae ipl1 protein kinases are known to cause abnormal chromosome segregation. we earlier isolated a cdna encoding a novel human protein kinase aik which shares high amino acid identity with the aurora/ipl1 protein kinase family. in the present study, a second human cdna highly homologous to aurora/ipl1 (aik2) was identified and the nucleotide sequence was determined (gene symbol stk12). the c-terminal kinase domain of the stk12 en ... | 1998 | 9858806 |
| assignment of galgt encoding beta-1, 4n-acetylgalactosaminyl-transferase (galnac-t) and kif5a encoding neuronal kinesin (d12s1889) to human chromosome band 12q13 by assignment to ici yac 26eg10 and in situ hybridization. medjph@stjames.leeds.ac.uk. | 1998 | 9858832 | |
| mint 3: a ubiquitous mint isoform that does not bind to munc18-1 or -2. | mint 1 and 2 are proteins that bind to munc18-1, an essential component of the synaptic vesicle fusion machinery, and are detectably expressed only in neurons [okamoto and südhof, j. biol. chem. 272, 31459-31464 (1997)]. mint 1 and 2 are composed of a variable n-terminal region that includes a conserved munc18-1-binding site, and a constant c-terminal region that contains one ptb and two pdz domains. we have now identified a third mint isoform, mint 3. similar to mint 1 and 2, the c-terminal hal ... | 1998 | 9860131 |
| isolation of growth suppressors from a cdna expression library. | we describe an experimental procedure for the isolation of growth inhibitory sequences from a complex cdna library. this approach first takes advantage of the setgap technique (selectable expression of transient growth arrest phenotype) to enrich for growth inhibitory sequences, followed by a screening procedure to identify individual cdnas that inhibit cell proliferation. here we provide a detailed description of the experimental protocol and report the characterization of two cdna sequences is ... | 1998 | 9872334 |
| characterization of the mouse src homology 3 domain gene sh3d2c on chr 7 demonstrates coexpression with huntingtin in the brain and identifies the processed pseudogene sh3d2c-ps1 on chr 2. | formation of intracellular protein complexes is often mediated by src homology 3 domain-containing proteins interacting with proline-rich target sequences on other proteins. the sh3d2c gene or its rat/human orthologs have been implicated in synaptic vesicle recycling due to interaction with dynamin i and synaptojanin in nerve terminals. in a yeast two-hybrid system, association with a huntingtin fragment containing an elongated stretch of polyglutamines was observed recently. by genetic mapping ... | 1998 | 9878254 |
| partial rescue of gata-3 by yeast artificial chromosome transgenes. | gata-3 is essential for murine embryonic development, but elucidating the genetic controls over the complex temporal and tissue-specific transcriptional regulatory pattern of this transcription factor gene has been problematic. here we report the isolation and characterization of two yeast artificial chromosomes (yacs) bearing the murine gata-3 gene. ordered deletions of both yacs show that they define a 1-megabase pair contig spanning the gata-3 locus. we found that a 120-kb yac transgene, incl ... | 1998 | 9882482 |
| high-level expression of human interleukin-17 in the yeast pichia pastoris. | human interleukin-17 (hil-17) gene without the signal sequence was isolated from activated peripheral blood lymphocytes by rt-pcr, then highly expressed in the yeast pichia pastoris in the form of the glycosylated monomer. the monomer of rhil-17 stimulated mouse fibroblast 3t3 cells to secrete il-6 and was specifically bound to its receptors on 3t3 cells. | 1998 | 9891843 |
| characterization of mouse ubiquitin-like smt3a and smt3b cdnas and gene/pseudogenes. | mouse smt3a and smt3b cdnas encoding ubiquitin-like proteins of 110 and 95 amino acids, respectively, were isolated and sequenced. the sequence of the first 92 amino acids (ending with the conserved gly-gly) of mouse smt3a exhibited two differences at amino acid no. 38 and 76 in comparison with that of human smt3a. the c-terminal 18 amino acid sequence of mouse smt3a was completely different from the c-terminal 11 amino acid sequence of human smt3a. mouse and human smt3b were identical for a seq ... | 1998 | 9891849 |
| differentially expressed genes in c6.9 glioma cells during vitamin d-induced cell death program. | c6.9 rat glioma cells undergo a cell death program when exposed to 1, 25-dihydroxyvitamin d3 (1,25-d3). as a global analytical approach, we have investigated gene expression in c6.9 engaged in this cell death program using differential screening of a rat brain cdna library with probes derived from control and 1,25-d3-treated cells. using this methodology we report the isolation of 61 differentially expressed cdnas. forty-seven cdnas correspond to genes already characterized in rat cells or tissu ... | 1998 | 10200452 |
| purification, characterization, and cloning of a cytosolic aspartyl aminopeptidase. | an aminopeptidase with a preference for n-terminal aspartyl and glutamyl residues but distinct from glutamyl aminopeptidase (ec 3.4. 11.7) was purified to near homogeneity from rabbit brain cytosol. its properties were similar to an enzyme described previously (kelly, j. a., neidle, e. l., and neidle, a. (1983) j. neurochem. 40, 1727-1734). aspartyl aminopeptidase had barely detectable activity toward simple aminoacyl-naphthylamide substrates. its activity was determined with the substrate asp-a ... | 1998 | 9632644 |
| the capacity of polyomavirus enhancer binding protein 2alphab (aml1/cbfa2) to stimulate polyomavirus dna replication is related to its affinity for the nuclear matrix. | the nuclear matrix is thought to play an important role in the dna replication of eukaryotic cells, although direct evidence for such a role is still lacking. a nuclear matrix-associated transcription factor, polyomavirus (py) enhancer binding protein 2alphab1 (pebp2alphab1) (aml1/cbfa2), was found to stimulate py replication through its cognate binding site. the minimal replication activation domain (rad) was identified between amino acid (aa) 302 and aa 371 by using a fusion protein containing ... | 1998 | 9632801 |
| identification, chromosomal mapping and tissue-specific expression of hrev3 encoding a putative human dna polymerase zeta. | the saccharomyces cerevisiae rev3 gene encodes the catalytic subunit of a non-essential dna polymerase zeta, which is required for mutagenesis. the rev3 mutants significantly reduce both spontaneous and dna damage-induced mutation rates. we have identified human cdna clones from two different libraries whose deduced amino acid sequences bear remarkable homology to the yeast rev3, and named this gene hrev3. the hrev3 gene was mapped to chromosome 1p32-33 by fluorescence in situ hybridization. the ... | 1998 | 9635887 |
| mutations in the nucleotide-binding sites of p-glycoprotein that affect substrate specificity modulate substrate-induced adenosine triphosphatase activity. | the amino- and carboxy-terminal nucleotide-binding domains (nbd1 and nbd2) of p-glycoprotein (p-gp) share over 80% sequence identity. almost all of nbd1 can be exchanged by corresponding nbd2 segments with no significant loss of function, except for a small segment around the walker b motif. within this segment, we identified two sets of residues [erga --> dkgt (522-525) and t578c] that, when replaced by their nbd2 counterparts, cause dramatic alterations of the substrate specificity of the prot ... | 1998 | 9636053 |
| molecular analysis of camnt1p, a mannosyl transferase important for adhesion and virulence of candida albicans. | there is an immediate need for identification of new antifungal targets in opportunistic pathogenic fungi like candida albicans. in the past, efforts have focused on synthesis of chitin and glucan, which confer mechanical strength and rigidity upon the cell wall. this paper describes the molecular analysis of camnt1, a gene involved in synthesis of mannoproteins, the third major class of macromolecule found in the cell wall. camnt1 encodes an alpha-1, 2-mannosyl transferase, which adds the secon ... | 1998 | 9636208 |
| multiple gene duplication and expression of mouse bcl-2-related genes, a1. | here we report the genomic cloning and characterization of the murine a1 genes, which belong to the bcl-2 gene family. southern analysis indicated the existence of at least four a1 genes in the murine genome and four different a1 genes, designated a1-a, -b, -c and -d, were cloned from the murine genomic library. the a1-a, -b and -d genes consisted of two exons, whereas the a1-c gene contained 1 bp insertion in the coding region which may result in an aberrant and truncated protein by frame-shift ... | 1998 | 9645611 |
| reduced joining of dna double strand breaks with an abnormal mutation spectrum in rodent mutants of dna-pkcs and ku80. | to characterize further the contribution of the dna-pk-dependent dsb repair pathway in mammalian cells. | 1998 | 9652803 |
| sequence analysis and expression of a novel mouse homolog of escherichia coli reca gene. | escherichia coli reca and its yeast homologs rad51 and dmc1 play crucial roles in mitotic and/or meiotic recombination and in repair of double-strand dna breaks. we have identified a murine novel reca-like gene (mmtrad). the predicted 329 amino acid protein showed significant homology to mouse rec2, rad51, dmc1 (or lim15) and e. coli reca. northern blot analysis revealed that mmtrad was ubiquitously transcribed in various tissues. | 1998 | 9655934 |
| functional reverse transcriptases encoded by a-type mouse line-1: defining the minimal domain by deletion analysis. | long interspersed elements, or lines, are retrotransposons that move via an rna intermediate. in mice, one polymorphic variant of l1 has amplified relatively recently, giving rise to the a-type subfamily in species belonging to the genus and subgenus mus. retrotransposition of line-1 (l1) requires the function of the l1-encoded reverse transcriptase that is produced from open reading frame 2 (orf2). here, we employ a convenient yeast genetic assay to determine the reverse transcriptase activity ... | 1998 | 9666081 |
| association of hsp105 with hsc70 in high molecular mass complexes in mouse fm3a cells. | the 105-kda stress proteins hsp105alpha and hsp105beta belong to a high molecular mass heat shock protein family which has been found in organisms from yeast to mammals. here we demonstrated the interaction of hsp105 with hsp70 family proteins in mouse fm3a cells. the association of hsp105 with hsc70 was shown by immunoprecipitation using anti-hsp105 antibody. furthermore, when cell extracts or partially purified hsp105 fractions from nonstressed or heat-shocked cells were analyzed by size exclu ... | 1998 | 9675148 |
| isolation of a gene encoding a novel member of the nuclear receptor superfamily from the critical region of usher syndrome type iia at 1q41. | 1998 | 9676434 | |
| nopp140 functions as a molecular link between the nucleolus and the coiled bodies. | coiled bodies are small nuclear organelles that are highly enriched in small nuclear rnas, and that have long been thought to be associated with the nucleolus. here we use mutational analysis, transient transfections, and the yeast two-hybrid system to show that the nucleolar phosphoprotein nopp140 functions as a molecular link between the two prominent nuclear organelles. exogenous nopp140 accumulated in the nucleolus rapidly, but only after a lag phase in coiled bodies, suggesting a pathway be ... | 1998 | 9679133 |
| molecular cloning and functional analysis of the arabidopsis thaliana dna ligase i homologue. | a cdna encoding the dna ligase i homologue has been isolated from arabidopsis thaliana using a degenerate pcr approach. the orf of this cdna encodes an amino acid sequence of 790 residues, representing a protein with a theoretical molecular mass of 87.8 kda and an isoelectric point (pi) of 8.20. alignment of the a. thaliana dna ligase protein sequence with the sequence of dna ligases from human (homo sapiens), murine (mus musculus), clawed toad (xenopus laevis) and the yeasts schizosaccharomyces ... | 1998 | 9681027 |
| characterization of kif1c, a new kinesin-like protein involved in vesicle transport from the golgi apparatus to the endoplasmic reticulum. | kinesins comprise a large family of microtubule-based motor proteins, of which individual members mediate specific types of motile processes. using the ezrin domain of the protein-tyrosine phosphatase ptpd1 as a bait in a yeast two-hybrid screen, we identified a new kinesin-like protein, kif1c. kif1c represents a member of the unc104 subfamily of kinesin-like proteins that are involved in the transport of mitochondria or synaptic vesicles in axons. like its homologues, the 1103-amino acid protei ... | 1998 | 9685376 |
| multiple functions of pmt1p-mediated protein o-mannosylation in the fungal pathogen candida albicans. | protein mannosylation by pmt proteins initiates o-glycosylation in fungi. we have identified the pmt1 gene and analyzed the function of pmt1p in the fungal human pathogen candida albicans. mutants defective in pmt1 alleles lacked pmt in vitro enzymatic activity, showed reduced growth rates, and tended to form cellular aggregates. in addition, multiple specific deficiencies not known in saccharomyces cerevisiae (including defective hyphal morphogenesis; supersensitivity to the antifungal agents h ... | 1998 | 9694829 |
| a retinoblastoma-binding protein that affects cell-cycle control and confers transforming ability. | the retinoblastoma (rb) gene is one of the most extensively studied tumour-suppressor genes. deletion or inactivation of both rb alleles is an essential, rate-limiting step in the formation of retinoblastoma and osteosarcoma that arise in families that carry mutant rb (ref. 2). rb inactivation is also found in other human tumours. whereas loss of rb function is associated with the loss of cellular proliferative control, introduction of a wild-type rb can suppress cell growth and tumorigenicity. ... | 1998 | 9697699 |
| cells deleted for brca2 cooh terminus exhibit hypersensitivity to gamma-radiation and premature senescence. | the putative brca2-mmrad51 interaction is analyzed in mouse cells deleted for the cooh terminus of brca2 (amino acids 3140-3328), which contains a region that associates with mmrad51 by yeast two-hybrid. these cells are hypersensitive to gamma-radiation (suggesting defective recombinational repair) but not uv light (suggesting intact nucleotide excision repair) and maintain the g1-s and g2-m checkpoints after exposure to gamma-irradiation. cells deleted for the cooh terminus of brca2 progress th ... | 1998 | 9699678 |
| analysis of repair and mutagenesis of chromium-induced dna damage in yeast, mammalian cells, and transgenic mice. | chromium (cr) is a widespread environmental contaminant and a known human carcinogen. we have used shuttle vector systems in yeast, mammalian cells, and transgenic mice to characterize the mutational specificity and premutational dna damage induced by cr(vi) and its reduction intermediates in order to elucidate the mechanism by which cr induces mutations. in the yeast system, treatment of vector-containing cells with cr(vi) results in a dose-dependent increase in mutations in the sup4-o target g ... | 1998 | 9703488 |
| anthrax lethal factor cleaves the n-terminus of mapkks and induces tyrosine/threonine phosphorylation of mapks in cultured macrophages. | lethal factor (lf) is the major virulence factor produced by bacillus anthracis. lf is sufficient to cause death in laboratory animals and cytolysis of peritoneal macrophages and macrophage cell lines. lf contains the characteristic zinc binding motif of metalloproteases and indirect evidence suggest that this hydrolytic activity is essential for its cytotoxicity. to identify the substrate(s) of lf, we have used the yeast two-hybrid system, employing a lf inactive mutant as bait. this approach h ... | 1998 | 9703991 |
| a 29-kilodalton golgi soluble n-ethylmaleimide-sensitive factor attachment protein receptor (vti1-rp2) implicated in protein trafficking in the secretory pathway. | expressed sequence tags coding for a potential snare (soluble n-ethylmaleimide-sensitive factor attachment protein receptor) were revealed during data base searches. the deduced amino acid sequence of the complete coding region predicts a 217-residue protein with a cooh-terminal hydrophobic membrane anchor. affinity-purified antibodies raised against the cytoplasmic region of this protein specifically detect a 29-kilodalton integral membrane protein enriched in the golgi membrane. indirect immun ... | 1998 | 9705316 |
| species identification and virulence attributes of saccharomyces boulardii (nom. inval.). | saccharomyces boulardii (nom. inval.) has been used for the treatment of several types of diarrhea. recent studies have confirmed that s. boulardii is effective in the treatment of diarrhea, in particular chronic or recurrent diarrhea, and furthermore that it is a safe and well-tolerated treatment. the aim of the present study was to identify strains of s. boulardii to the species level and assess their virulence in established murine models. three strains of s. boulardii were obtained from comm ... | 1998 | 9705402 |
| the a gamma-globin 3' element provides no unique function(s) for human beta-globin locus gene regulation. | the human beta-globin locus is activated transcriptionally by a complex series of events that culminate in appropriate temporal and tissue-specific control over five separate genes during embryonic and early postnatal development. one cis-regulatory element in the locus, originally identified as an enhancer 3' to the agamma-globin gene, more recently has been suggested to harbor alternative or additional properties, including stage-specific silencer, insulator, nuclear matrix, or chromosome scaf ... | 1998 | 9707580 |
| the 5'-amp-activated protein kinase inhibits the transcriptional stimulation by glucose in liver cells, acting through the glucose response complex. | 5-amino-4-imidazolecarboxamide riboside (aicar) is known to stimulate rat liver 5'-amp-activated protein kinase (ampk). ampk is the mammalian homologue of snf1p in yeast, involved in derepression of glucose-repressed genes. we used aicar to test if ampk could also play a role in the regulation of glucose-dependent genes in mammalian cells. at a concentration which induces phosphorylation-dependent inactivation of hmg-coa reductase, aicar blocked glucose activation of three glucose responsive gen ... | 1998 | 9708898 |