| species composition and geographic distribution of ticks infesting cattle, goats and dogs in a temperate and in a subtropical region of south-east africa. | the species and distribution of ticks infesting cattle, goats and dogs in the eastern region of the eastern cape province, south africa and maputo province, mozambique were determined from collections made from these animals at 72 localities in the former region and 30 in the latter. eleven ixodid and one argasid species were recovered in the eastern cape province and 15 ixodid species in maputo province. the most common ticks infesting cattle and goats in both provinces were amblyomma hebraeum, ... | 2009 | 21105593 |
| recombination is a major driving force of genetic diversity in the anaplasmataceae ehrlichia ruminantium. | the disease, heartwater, caused by the anaplasmataceae e. ruminantium, represents a major problem for tropical livestock and wild ruminants. up to now, no effective vaccine has been available due to a limited cross protection of vaccinal strains on field strains and a high genetic diversity of ehrlichia ruminantium within geographical locations. to address this issue, we inferred the genetic diversity and population structure of 194 e. ruminantium isolates circulating worldwide using multilocus ... | 2016 | 27747194 |
| isolation, cloning and structural characterisation of boophilin, a multifunctional kunitz-type proteinase inhibitor from the cattle tick. | inhibitors of coagulation factors from blood-feeding animals display a wide variety of structural motifs and inhibition mechanisms. we have isolated a novel inhibitor from the cattle tick boophilus microplus, one of the most widespread parasites of farm animals. the inhibitor, which we have termed boophilin, has been cloned and overexpressed in escherichia coli. mature boophilin is composed of two canonical kunitz-type domains, and inhibits not only the major procoagulant enzyme, thrombin, but i ... | 2008 | 18286181 |
| structure and mode of action of microplusin, a copper ii-chelating antimicrobial peptide from the cattle tick rhipicephalus (boophilus) microplus. | microplusin, a rhipicephalus (boophilus) microplus antimicrobial peptide (amp) is the first fully characterized member of a new family of cysteine-rich amps with histidine-rich regions at the n and c termini. in the tick, microplusin belongs to the arsenal of innate defense molecules active against bacteria and fungi. here we describe the nmr solution structure of microplusin and demonstrate that the protein binds copper ii and iron ii. structured as a single alpha-helical globular domain, micro ... | 2009 | 19828445 |
| comparing the detection of exposure to ehrlichia ruminantium infection on a heartwater-endemic farm by the pcs20 polymerase chain reaction assay and an indirect map1-b enzyme linked immunosorbent assay. | detection of heartwater is not always easy especially because all the serological assays so far available either have poor sensitivity or specificity. the indirect map-1b elisa has been reported to be the most specific test for heartwater, although it does also detect antibodies to some closely related ehrlichial agents. this study was undertaken to compare two methods for the detection of heartwater infection caused by the ehrlichial agent ehrlichia (cowdria) ruminantium. fifteen cattle on a he ... | 2003 | 14621319 |