Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [the persistence of activity of h1n1 (swine) influenza virus in pig breeding units during non-epidemic phases]. | a study is carried on in a group of 16 commercial breeding-finishing units in brittany (france). the aim of this study is to try to reveal the persistence of activity of the influenza virus within these intensive units after an acute epizootic. in each farm two batches of pigs are selected, individually identified and followed from suckling period to slaughter. serological controls are conducted every month on the same pigs. both influenza and aujeszky's disease antibodies are apprehended. furth ... | 1985 | 3004802 |
| characterization and mapping of a nonessential pseudorabies virus glycoprotein. | antigenic variants of pseudorabies virus (prv) containing mutations in a viral glycoprotein with a molecular weight of 82,000 (giii) were isolated by selecting for resistance to a complement-dependent neutralizing monoclonal antibody (mca82-2) directed against giii. these mutants were completely resistant to neutralization with mca82-2 in the presence of complement. two mutants selected for further studies either did not express giii or expressed an improperly processed form of the glycoprotein. ... | 1986 | 3005646 |
| dot-enzyme immunoassay for visual detection of antibodies to pseudorabies virus in swine serum. | a modified solid-phase enzyme immunoassay (eia) is described for the visual detection of anti-pseudorabies virus (anti-prv) antibody in porcine serum. dots of prv antigens were adsorbed to nitrocellulose paper (hence the name dot-eia), and the remaining nonspecifically reactive sites were blocked with bovine serum albumin or skim milk powder. after immersion in test serum, bound antibodies were reacted with a peroxidase-conjugated anti-porcine immunoglobulin g (h & l). positive reactions were ea ... | 1986 | 3007569 |
| [induction of biological protection in pigs against infection with aujeszky disease virus by vaccination with large doses of live or inactivated vaccines]. | pigs were inoculated against the aujeszky's disease twice in a four-week interval. the dose of the live vaccine was 10(6) tkid50 and the titres of neutralizing antibodies were 1 : 16 to 1 : 128 in blood serum. two weeks later the pigs were exposed to contact infection. primary multiplication of the virus was observed on the mucous membranes of the nose and oropharynx and the virus was detected on the nasal mucous membrane within one to five days, the maximum infection titre values being 10(1.3) ... | 1986 | 3008403 |
| control of aujeszky's disease. | 1986 | 3010537 | |
| evaluation of a diagnostic antigen for the detection of aujeszky's disease virus-infected subunit-vaccinated pigs. | an early virus protein complex that is found in the maintenance medium of aujeszky's disease (ad) virus-infected cells was evaluated as a subunit diagnostic antigen (suda) in the enzyme-linked immunosorbent assay (elisa). this antigen was found in purer form and in larger quantities for up to 12 h post-infection in the maintenance medium of ad virus-infected mdbk cell cultures than in the maintenance medium of virus-infected porcine fallopian tube (pft) and pk1a cell cultures. the suda was shown ... | 1986 | 3010548 |
| [comparative studies of the detection of aujeszky virus from filtered and unfiltered study samples]. | 1986 | 3011385 | |
| differentiation of serum antibodies from pigs vaccinated or infected with aujeszky's disease virus by a competitive enzyme immunoassay. | a competitive enzyme immunoassay was developed to detect antibodies to a glycoprotein (gi) of aujeszky's disease virus. infected cell monolayers were used as antigen and a monoclonal antibody directed against an epitope of gi as indicator antibody. it was demonstrated that pigs vaccinated with the bartha, buk or nia-4 strains did not produce antibody to the epitope of gi, whereas all wild-type viruses tested did induce this antibody. the antibody to the gi epitope persisted for at least 15 weeks ... | 1986 | 3011974 |
| vaccine engineering. violation of rules alleged. | 1986 | 3012349 | |
| aujeszky's disease: blocking elisa for the detection of serum antibodies. | a blocking elisa for the detection of serum antibodies to aujeszky's disease virus has been developed. the test was designed in particular with a view to examining large numbers of blood samples. it has been found to be sensitive, specific and precise. in comparison with the serum neutralization test it was superior in detecting low levels of antibodies in pig sera. the blocking elisa gave higher titre values in serum samples taken early after experimental infection of pigs with aujeszky's disea ... | 1986 | 3016016 |
| pseudorabies virus gene encoding glycoprotein giii is not essential for growth in tissue culture. | we have established that in the becker strain of pseudorabies virus (prv), the glycoprotein giii gene is not essential for growth in cell culture. this was accomplished by construction and analysis of viral mutants containing two defined deletion mutations affecting the giii gene. these mutations were first constructed in vitro and introduced into escherichia coli expression plasmids to verify structure and protein production. each mutation was then crossed onto prv by cotransfection of plasmid ... | 1986 | 3016326 |
| immunoperoxidase plaque staining for the detection of pseudorabies virus. | a quantitative indirect immunoperoxidase plaque staining method was developed for the detection of pseudorabies virus infection in a pig kidney cell line (pk-15). the method is rapid and specific and foci of infection, represented by stained plaques, are easily counted by the unaided eye. possible modification of this technique in a plaque reduction assay for the detection of antipseudorabies virus antibody is also discussed. | 1986 | 3017523 |
| development and evaluation of an indirect enzyme immunoassay for detection of porcine antibodies to pseudorabies virus. | an indirect enzyme immunoassay is described for detection of porcine serum antibody to pseudorabies virus. the analytical sensitivity of the enzyme immunoassay was found to be approximately 4.5 log 4 x 10 (5120 times) greater than the serum neutralization test, based on parallel end point titrations. the diagnostic sensitivity of the enzyme immunoassay was comparable or superior to that of the serum neutralization, based on the earliest detectable antibody after infection of swine with pseudorab ... | 1986 | 3017531 |
| [electron microscopy of aujeszky's disease virus in explants of gasser's ganglion from pigs with a latent infection]. | different developmental stages of the aujeszky's disease virus were demonstrated by electron microscopy in the ultra-thin slices by the cultivated fragments of the gasserian ganglion (g. g.) of two pigs latently infected with the aujeszky's disease virus (adv). in a pig vaccinated with the inactivated vaccine against the disease, the virus was detected in the g. g. cells 186 days after virus challenge, the reactivation of latency being obtained after immunosuppression with dexamethasone. in the ... | 1986 | 3020761 |
| phagocytosis and intracellular killing of pasteurella multocida by porcine alveolar macrophages after infection with pseudorabies virus. | the purpose of this study was to determine if pseudorabies virus (prv) interfered with normal alveolar macrophage phagocytic functions. porcine alveolar macrophages (pam) obtained by pulmonary lavage were exposed to prv. at 1 hour postinfection, cells were challenged with pasteurella multocida labeled with 3[h] thymidine. the phagocytosis assay was performed by measuring total radioactivity 1 hour after pm challenge in a soft-beta spectrophotometer. intracellular killing was measured by counting ... | 1986 | 3020772 |
| antiviral effectiveness of butylated hydroxytoluene against pseudorabies (aujeszky's disease) virus in cell culture, mice, and swine. | butylated hydroxytoluene (bht) was evaluated for antiviral effectiveness on pseudorabies virus (prv) in cell culture, mice, and swine. when relatively small amounts of bht were mixed with prv and incubated at 37 c for 30 or 60 minutes before inoculation into cell cultures, the cell cultures did not become infected with virus. the prv was not infectious when the virus was treated with bht and then inoculated intraperitoneally into mice, but was infectious when bht and prv were inoculated simultan ... | 1986 | 3021025 |
| researcher reprimanded for pseudorabies test. | 1986 | 3022375 | |
| [use of immunoenzyme (elisa) diagnosis of aujeszky's disease in pigs during the recovery period]. | samples of blood and blood serums of pigs were examined for the presence of antibodies to the aujeszky's disease virus. the virus-neutralizing (vn) test and the enzymoimmunologic (elisa) method were used for this examination. as indicated by comparison of the average titres of antiviral antibodies determined by both methods, the elisa method is 60 to 600 times more sensitive than the vn test. the high sensitivity of the elisa method enabled to detect antiviral antibodies even in samples consider ... | 1986 | 3022451 |
| herpesvirus (pseudorabies virus) latency in swine: occurrence and physical state of viral dna in neural tissues. | the occurrence of the pseudorabies virus (prv, herpes suis 1) genome in various neural tissues of latently infected pigs was investigated. during the latent phase of infection, between 7 and 52 weeks p.i., the average amount of prv dna ranged between 0.3 and 0.05 genome copies per cell. the results obtained by in situ cytohybridization and reassociation kinetic experiments indicated that each latently infected cell harbored at least 30 viral genome copies. prv dna could be demonstrated in simila ... | 1986 | 3024403 |
| detection of latent pseudorabies virus in porcine tissue, using a dna hybridization dot-blot assay. | a dna-hybridization dot-blot technique was developed to detect the presence of pseudorabies virus (prv) dna in porcine tissue. seven 32p-nick translated probes of high specific activity were prepared from transformed escherichia coli plasmids into which bacillus amyloliquefaciens h (bam hi) restriction fragments of prv-dna had been inserted. samples of dna that had been extracted from porcine tissue or from prv grown in tissue culture were transferred to nitrocellulose paper, using a microsample ... | 1986 | 3024534 |
| aujeszky's disease vaccination and infection of pigs with maternal immunity: effects on cell- and antibody-mediated immunity. | scc, adv-scc, adv-adcc and adv-lyst as well as nd50-titres of neutralizing serum antibodies were examined in 36 passively immune pigs, 25 of which were vaccinated at 3 weeks of age and partly revaccinated 3 weeks later. twenty-five vaccinated animals and 8 non-immune control pigs were challenged with infectious adv. independent of the state of maternal immunity the cytotoxic response of the white blood cells from all the animals was low at wpp 3 but rose with increasing age. adv-lyst occurred on ... | 1987 | 3026291 |
| genome location and identification of functions defective in the bartha vaccine strain of pseudorabies virus. | we have shown previously (lomniczi et al., j. virol. 52:198-205, 1984) that the bartha vaccine strain of pseudorabies virus has a deletion in the short unique (us) region of its genome--a deletion that is related to the absence of virus virulence. this strain is, however, also defective in other genes involved in virulence. we show here that virulence can be restored by marker rescue of the bartha strain to which an intact us has been restored (but not to the parental bartha strain) by sequences ... | 1987 | 3027406 |
| construction and characterization of deletion mutants of pseudorabies virus: a new generation of 'live' vaccines. | various deletions were introduced into a cloned subgenomic fragment (bamhi-7), located in the unique short (us) region of the dna from the virulent northern ireland aujeszky-3 (nia-3) strain of pseudorabies virus (prv). in the cloned hindiii-b fragment, the mlui-bglii fragment was replaced by different mlui-bglii fragments of the deleted bamhi-7 clones. transfection of the deleted hindiii-b fragments together with the hindiii-a fragment of either the nia-3 or the non-virulent nia-4 strain yielde ... | 1987 | 3029289 |
| intranasal vaccination of pigs against aujeszky's disease: comparison with one or two doses of attenuated vaccines in pigs with high maternal antibody titres. | ten-week-old pigs with high levels of maternally derived antibody (mda) against aujeszky's disease virus (adv) were given either a single intranasal vaccination or one or two doses (with an interval of three weeks) of commercially available attenuated adv vaccines intramuscularly. the pigs did not produce a clear neutralising antibody response to adv. however, pigs vaccinated intranasally and pigs given two doses of attenuated adv vaccines were protected against intranasal challenge with virulen ... | 1987 | 3029845 |
| vaccination against aujeszky's disease by different routes using live attenuated and inactivated virus vaccines in pigs with or without passive immunity. | 1986 | 3030027 | |
| [comparison of the biological properties of various strains of aujeszky's disease viruses]. | we compared three strains of aujeszky's disease virus that had been isolated from slaughter pigs from the dormant foci of aujeszky's disease, with the known virulent strain cvos and top. no significant differences were found out in the following characters: titer value on tissue cultures from chick embryonal cells (keb) and cellular line mdbk at the temperatures of 37 degrees c and 40 degrees c, cytopathogenic effect (cpe) in the hela cells, plaque formation in the keb tissue culture, pruritus r ... | 1987 | 3031866 |
| four-layer enzyme immunoassay (eia) detection of differences in igg, igm and iga antibody response to aujeszky's disease virus in infected and vaccinated pigs. | the use of the four-layer enzyme immunoassay (eia) for the detection of igg, igm and iga antibodies against aujeszky's disease virus in blood and oropharyngeal swabs of infected and vaccinated pigs is described. mean antibody titres obtained using the four-layer eia were 6.1 and 3829 times higher compared with the indirect enzyme-linked immunosorbent assay (elisa) and virus neutralization (vn) test, respectively. the vn test detected mainly igg antibodies, while the igm antibodies did not react. ... | 1987 | 3031872 |
| the properties of aujeszky's disease (pseudorabies) virus after serial passages in chick-embryo cell cultures in the presence of ethyl p-chlorphenoxyisobutyrate (cpib). | 1986 | 3031907 | |
| swine antibody-dependent cellular cytotoxicity against pseudorabies virus-infected cells. | in pseudorabies virus (prv) infection of pigs, antibody-dependent cellular cytotoxicity (adcc) may be an early defense mechanism. peripheral blood leukocytes (pbl) and pulmonary macrophages mediate adcc activity. antibody-dependent cellular cytotoxicity against prv-infected target cells was assessed, and the effect of infection of cells having an adcc-effector function was determined. although pulmonary lavage cells (plc) had adcc activity, in vitro infection of plc led to prv replication, loss ... | 1987 | 3032027 |
| a necrotizing pneumonia in lambs caused by pseudorabies virus (aujesky's disease virus). | an outbreak of pseudorabies occurred in sheep housed with swine in the same building. although the sheep and swine were not in physical contact, the lambs and ewes were exposed to air from the sows' section. three dead lambs were submitted to the iowa state university veterinary diagnostic laboratory for necropsy. grossly there were pulmonary congestion and multifocal pulmonary hemorrhages. microscopic lesions were severe acute multifocal necrotizing bronchopneumonia with necrotizing vasculitis ... | 1987 | 3032388 |
| evolution of pseudorabies virions containing genomes with an invertible long component after repeated passage in chicken embryo fibroblasts. | the genome of pseudorabies virus consists of two components, short (s) and long (l). only the s component is bracketed by inverted repeats, and only the s component inverts itself relative to the l component, giving rise to two isomeric forms of the genome. an attenuated vaccine strain of pseudorabies virus (norden), however, has a genome which is found in four isomeric forms (b. lomniczi, m. l. blankenship, and t. ben-porat, j. virol. 49:970-979, 1984). to determine the basis for the atypical s ... | 1987 | 3033309 |
| induction and characterization of swine beta interferon. | newcastle disease virus (ndv) strain "h" and polyinosinic-polycytidylic acid (poly i:c) were used for interferon (ifn) induction in secondary pig kidney cells. a functional ifn system was detected and characterized. a wide similarity with the correspondent human and bovine systems was appreciated, with particular regard to the kinetics of synthesis. a glycosylated protein was essential for activity in bovine cells, but not in swine cells. poly i:c proved to be a very weak inducer, even in condit ... | 1987 | 3034503 |
| restriction endonuclease analysis of aujeszky's disease viruses isolated in japan. | 1987 | 3035261 | |
| local humoral and cellular responses in aujeszky's disease virus infection in pigs. | mucosal and tracheal washings from pigs vaccinated parenterally and intranasally with aujeszky's disease virus were tested for specific anti-aujeszky's disease virus responses. antibody tests included complement dependent antibody lysis, antibody dependent cellular cytotoxicity, virus neutralisation, and anti-aujeszky's disease virus iga and igg levels. there was no correlation between the levels of these antibodies and protection from subsequent challenge. direct lymphocyte cytotoxicity against ... | 1987 | 3035662 |
| the role of acute and latent virus infections in the pathogenesis of inner ear disturbances. | the possible role of herpesviral infections of the inner ear in suddenly appearing inner ear disturbances was investigated. experimental pseudorabies virus (prv, herpes sui 1) infection of mice and swine was used as a model system. infected mice represented the productive cycle of prv infection (acute phase), whereas the latent phase of infection could be tested in swine. from the acutely infected mice the virus could be reisolated from perilymphatic fluid and various parts of the brain. massive ... | 1987 | 3035932 |
| second-generation pseudorabies virus vaccine with deletions in thymidine kinase and glycoprotein genes. | a modified-live pseudorabies virus (prv) vaccine, designated prv(dlg92/d1tk), with deletions in the thymidine kinase (tk) and glycoprotein-giii (g92) genes, was derived from the prv (bucharest [buk]-d13) vaccine strain. the vaccine virus also contained a deletion in glycoprotein gi. despite 3 deletions, prv(dlg92/d1tk) replicated to high titers in cell culture from 30 c to 39.1 c. enzyme assays and autoradiography revealed that prv(dlg92/d1tk) did not induce a functional tk activity in infected ... | 1987 | 3035972 |
| [the effect of colostral immunity on the active formation of antibodies in pigs after the administration of inactivated vaccine against aujeszky's disease]. | in a large herd of pigs where a trial was performed to cure the animals from aujeszky's disease (ad) by applying to all animals an inactivated vaccine, a post-vaccination antibody response was studied in piglets coming from the sows that were vaccinated several times. when the piglets were vaccinated at the age of eight weeks (the average virus-neutralizing titer (vnt) of colostral antibodies was 1:11.4) and revaccinated at the age of 11 weeks, 73% of the forty-five animals (examined at the age ... | 1987 | 3037756 |
| pathological changes in hpcd pigs with prednisolone induced recrudescence of pseudorabies virus. | in hpcd pigs inoculated with prv, latent prv could be reactivated in-vivo by the administration of large doses of prednisolone 3 months after the primary infection. in two pigs, virus shedding was without clinical signs of disease, whereas depression of circulating lymphocytes was prominent. reactivation of prv was also demonstrated by cultivation of the brain cortex on the 7th day and the mandibular lymph node on the 9th day after the prednisolone began treatment. coincident with the virus isol ... | 1987 | 3038971 |
| programs for the elimination of pseudorabies virus from large herds of swine. | 1988 | 3403349 | |
| effects of experimental pseudorabies virus infection on vaccinated pregnant sows. | in regard to field problems, a study was done to determine the effects of an infection with virulent pseudorabies virus (prv) on pregnant sows vaccinated with an inactivated virus vaccine. three routes of viral inoculation have been tested: intranasal, intra-uterine and intra-fetal. the nasal infection did not allow to induce pregnancy disorders observed on the field on pregnant sows. after insemination with semen mixed with prv, abortion was observed on one of the two immune sows. in this one, ... | 1987 | 3426109 |
| recurrent viruses in a singapore intensive pig farming estate. | the ponggol intensive pig farming estate was conceived approximately 10 years ago to accommodate pig farms resettled from water catchment areas. initially stocking density of the estate was 1300-1500 pigs per hectare (ha) and the total pig population in the estate was 200,000. over the past 10 years the numbers of pigs have increased to 400,000 and the stocking density of the big commercial units have increased to 1500-2500 per ha. with such intensive methods of husbandry environmental problems ... | 1987 | 3446006 |
| genetic control of immune response to pseudorabies and atrophic rhinitis vaccines: i. heterosis, general combining ability and relationship to growth and backfat. | data from 988 pigs from 119 litters farrowed in two seasons of a three-breed diallel crossbreeding experiment were analyzed to estimate general combining abilities of breeds and heterosis for humoral immune response to pseudorabies virus and atrophic rhinitis vaccines. twenty purebred boars and 85 sows of the duroc, landrace and yorkshire breeds were mated to provide the nine breed-of-sire and breed-of-dam combinations. immune response was measured after vaccination. a modified-live pseudorabies ... | 1987 | 3558146 |
| genetic control of immune response to pseudorabies and atrophic rhinitis vaccines: ii. comparison of additive direct and maternal genetic effects. | data from 52 litters farrowed in two seasons of a cross-fostering experiment were analyzed to estimate variances and covariances for additive direct and maternal genetic effects on immune response to pseudorabies virus and b. bronchiseptica vaccine. twenty purebred boars and 44 sows of the duroc, landrace and yorkshire breeds were used. immune response was measured after vaccine challenge. a modified-live pseudorabies (pr) vaccine was administered to piglets at 28 d of age; response was measured ... | 1987 | 3558147 |
| porcine effector mechanisms: antibody-dependent cell-mediated cytotoxicity of pseudorabies-infected target cells. | antibody-dependent cell-mediated cytotoxicity (adcc) against pseudorabies virus (prv)-infected cells was determined in neonatal porcine pulmonary washout cells and in partially purified blood-derived leukocytes. the adcc assays were performed at approximately 1, 2, 3 and 11 days after birth. it was found that pulmonary-derived cells had a low level of cytolytic activity against the prv-infected target cells at about 1 day of age. this activity increased sharply during the next 2 days of age, but ... | 1987 | 3590615 |
| pneumonia in pigs induced by intranasal challenge exposure with pseudorabies virus and pasteurella multocida. | the interaction between pseudorabies virus (prv) and pasteurella multocida was investigated to determine whether single or combined infections result in pneumonia in 6- to 7-week-old pigs. the effect of the prv-p multocida challenge exposure on feed consumption, rate of gain, and extent of pneumonic lesions appeared dependent on the prv dose; however, pneumonic lesions were of bacterial pneumonia. pigs inoculated with a virulent strain of prv plus p multocida developed severe pneumonia, whereas ... | 1987 | 3674553 |
| severe pleuritis associated with certain strains of pasteurella multocida in swine. | pasteurella multocida was isolated from 2 farms on which grower or finisher pigs had problems of severe emaciation and high death loss (greater than 5%). at necropsy, the pigs had extensive suppurative pleuritis and pericarditis, with adhesions over the lung surface. on one farm, the pigs also had multiple lung abscesses. histologic findings included polymorphonuclear cell infiltration in bronchial and alveolar spaces, thickening of alveolar walls, pleuritis, and in some cases, abscesses. from a ... | 1987 | 3679971 |
| inhibition of african swine fever infection in the presence of immune sera in vivo and in vitro. | in assay cultures, sera from african swine fever convalescent pigs inhibited infection by homologous african swine fever virus. the infection-inhibition capacity did not correspond with the virus-neutralizing capacity. the serum did not prevent infection by heterologous virulent viruses. sera from pigs challenge inoculated with the homologous virulent virus and later with a heterologous virulent virus inhibited the infection by different heterologous virulent viruses. these sera did not interfer ... | 1986 | 3729125 |
| levels of passive antibodies against aujeszky's disease virus in piglets derived from infected sows. | 1986 | 3735896 | |
| single-dilution indirect solid-phase radioimmunoassay for the detection of anti-pseudorabies immunoglobulin g in swine sera. | a single-dilution indirect solid-phase radioimmunoassay (iria) was developed for the detection of low levels of anti-pseudorabies immunoglobulin g in swine sera. the assay derived increased sensitivity from the use of a second amplifying antibody. the iria was examined for its stoichiometry, amplification by secondary antibody, advantage of a single-dilution assay vs an end-point titration, sensitivity, and specificity. the assay had a near linear dose-response relationship with positive sera (s ... | 1986 | 3740619 |
| functional antibody responses in pigs vaccinated with live and inactivated aujeszky's disease virus. | functional antibody tests, including virus neutralising activity of serum, antibody dependent cellular cytotoxicity and complement mediated lysis, were used to measure the response of pigs given either live or inactivated aujeszky's disease virus vaccines. pigs were then challenged with virulent aujeszky's disease virus and antibody responses were analysed and found not to correlate with protection. reasons for this lack of correlation are discussed and it is suggested that these results indicat ... | 1986 | 3809723 |
| pseudorabies virus infection in piglets accompanying with the lesion of bilateral encephalomalacia. | 1985 | 3981837 | |
| eradication of some infectious pig diseases by perinatal tiamulin treatment and early weaning. | from 10 days before the expected date of farrowing onwards, 97 sows infected by mycoplasma hyopneumoniae and treponema hyodysenteriae were given tiamulin daily at a dosage of 20 mg/kg bodyweight via the feed. three days before farrowing the sows were washed with a disinfectant and transferred to an isolated farrowing house. the sucking piglets remained with their dams for five days, during which time the sows continued to receive the tiamulin-containing feed. the sucking piglets also received ti ... | 1985 | 3984171 |
| properties of aujeszky's disease virus strains isolated from swine pneumonia cases. prelininary report. | 1968 | 4298985 | |
| swine interferon. i. induction in porcine cell cultures with viral and synthetic inducers. | the production of interferon by porcine kidney (pk(15)) cell culture in response to viral and synthetic inducers was studied. the inducers used included a synthetic double-stranded polyribonucleotide, polyriboinosinic-polyribocytidylic acid (poly i:c), swine influenza virus and three strains of pseudorabies virus. following exposure to these inducers cell culture fluids were examined for interferon by the plaque-reduction method. the poly i:c and the swine influenza virus induced production of i ... | 1972 | 4340345 |
| the histopathology of experimental pneumonia in pigs produced by aujeszky's disease virus. | 1973 | 4375835 | |
| ultrastructural changes in the lungs of pigs infected with aujeszky's disease virus. | 1973 | 4375836 | |
| pseudorabies virus with lost virulence for rabbits after intra-muscular inoculation, obtained from persistently infected cell cultures. | 1968 | 4385165 | |
| resistance of pig tonsillary and throat mucosa to reinfection with a homologous pseudorabies virus strain. | 1970 | 4392072 | |
| the effect of colostrum derived antibody on mortality and virus excretion following experimental infection of piglets with aujeszky's disease virus. | 1973 | 4803575 | |
| biological properties of aujeszky's disease (pseudorabies) virus strains with special regard to interferon production and interferon sensitivity. | 1974 | 4837359 | |
| inhibition of herpes virus-induced cell fusion by concanavalin a, antisera, and 2-deoxy-d-glucose. | pseudorabies virus-induced cell fusion in rabbit kidney cells can be prevented by concanavalin a added early after infection. the infected cells are not agglutinated and the infectivity of cell-free virus is not reduced. sera from productively infected animals also inhibit polykaryocytosis, whereas a hyperimmune serum directed against virus structural components has no effect. 2-deoxy-d-glucose reversibly disturbs virus-induced fusion and reduces significantly the virus infectivity. | 1974 | 4858786 |
| [viability of the buk vaccine strain of aujeszky's disease virus in the aerosol state]. | 1970 | 5421600 | |
| stability of the pseudorabies virus genome after in vivo serial passage. | restriction endonuclease patterns of pseudorabies virus (prv) dna were examined after each of 11 serial passages of the virus through pigs. minor variations in the electrophoretic mobility of certain restriction enzyme fragments were observed by the sixth passage. this variability was similar to some of the minor variability observed in field isolates. the variable fragments were mapped to three locations on the prv genome: the junction between the short unique sequences and the repeat sequences ... | 1984 | 6086823 |
| [resistance of aujeszky disease viruses, present in the blood of pigs, to potassium thiosulphate and hydrogen chloride]. | a trial was conducted to study the sensitivity of the aujeszky disease virus, present in the blood of pigs, to the effect of two preservatives: potassium thiosulphate ( k2s2o5 ) and hydrogen chloride (hcl). the sensitivity of the virus was determined on rabbits infected with pig blood containing aujeszky disease virus with different additions of the two preservatives. as found, 0.5% of k2s2o5 and 3% of hcl are concentrations fully sufficient for the inactivation of all the viruses present in the ... | 1984 | 6087536 |
| influence of vaccination on aujeszky's disease virus and disease transmission. | parenteral vaccination of fattening pigs with either modified live or inactivated aujeszky's disease virus did not prevent infection with field strain virus or the development of clinical disease. the duration and severity of the clinical syndrome was, however, reduced and vaccinated pigs did not suffer the severe weight loss and high mortality experienced by non-vaccinated pigs in the acute phase of disease. the range of tissues in which challenge virus replication took place was more restricte ... | 1984 | 6089404 |
| testicular changes observed in boars following experimental inoculation with pseudorabies (aujeszky's) virus. | four boars were inoculated intranasally with pseudorabies virus to determine if microscopic testicular changes occurred as a result of infection. testicular biopsies and semen samples were taken at two, four and six weeks postinoculation and the boars were castrated immediately after the last sample collection. testicular samples and semen were cultured to determine if the virus was present. pseudorabies virus was not isolated from the semen or testicular tissue. virus was isolated from trigemin ... | 1984 | 6089983 |
| swine diseases transmissible with artificial insemination. | the transport of fresh and frozen semen to be used for artificial insemination creates a mode of disease transmission between farms. normally, semen contains a number of nonpathogenic bacterial contaminants; however, excessive bacterial contamination can result in infertile matings. contamination with a known pathogen, eg, brucella suis, could initiate a serious outbreak of disease in a recipient herd. methods to minimize bacterial contamination of semen include sanitary collecting and processin ... | 1984 | 6090372 |
| construction of e. coli expression plasmid libraries: localization of a pseudorabies virus glycoprotein gene. | we describe the use of a combined cloning/expression protocol to identify a gene encoding a pseudorabies virus (prv) glycoprotein. prior to this study, the genome locations of prv glycoproteins had not been described. we first identified prv glycoproteins using antibodies directed against prv virions. using affinity chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the prv glycoproteins were separated and antibodies were made against them in rabbits. using dnase diges ... | 1984 | 6090566 |
| genetic basis of the neurovirulence of pseudorabies virus. | lomniczi et al. (j. virol. 49:970-979, 1984) have shown previously that two attenuated vaccine strains of pseudorabies virus have a similar deletion in the short unique (us) region of the genome. the region which is deleted normally codes for several translationally competent mrnas. as expected, these mrnas are not formed in the cells infected with the vaccine strains. the function specified by these mrnas is thus not necessary for growth in cell culture. using intracerebral inoculation of 1-day ... | 1984 | 6090697 |
| [epidemiology of aujeszky's disease in switzerland]. | 1984 | 6091267 | |
| vaccination against aujeszky's disease: field experiences. | twelve herds were investigated where outbreaks of clinical aujeszky's disease had occurred. clinical aujeszky's disease was eliminated from all farms following vaccination. after vaccination was stopped in two of the six fattening herds virus was also apparently eradicated, judging from serological examination. these two herds were the smallest of the six fattening farms and size and the throughput of pigs may have contributed to apparent eradication of virus. in two of six breeding farms where ... | 1984 | 6093329 |
| some characteristics of four attenuated vaccine virus strains and a virulent strain of aujeszky's disease virus. | in the present study four attenuated virus strains, used as vaccines, and a virulent strain of aujeszky's disease virus (adv) were compared with respect to their virulence in mice, their ability to induce virus-specified thymidine kinase (tk) in infected cells, and their cleavage profiles of viral dna's after treatment with the restriction endonuclease kpni. the survival time of mice inoculated with the b-kal or the virulent nia-3 strain was comparable, whereas the bartha and buk strains require ... | 1984 | 6093331 |
| evaluation of field isolates of pseudorabies (aujeszky's disease) virus as determined by restriction endonuclease analysis and hybridization. | sixty-seven isolates of pseudorabies virus (prv) from 13 states were cleaved with 4 restriction endonucleases (re), and after electrophoresis in agarose, their banding patterns were photographed and evaluated. the deoxyribonucleic acid (dna) cleavage fragments were designated into regions specified by molecular weight ranges based on lambda phage dna as a size marker. the 67 prv isolates were evaluated according to the total number of cleavage fragments, by the number of fragments within designa ... | 1984 | 6093637 |
| radial immunodiffusion enzyme assay for detection of antibodies to pseudorabies virus in swine serum. | a radial immunodiffusion enzyme assay for the detection and quantitation of antibodies to pseudorabies virus in swine sera was developed and the methods were standardized. the assay combined the principle of radial immunodiffusion with enzyme-linked immunosorbent assay. quantitation of pseudorabies virus antibody titers was determined by measuring the diameter of a colored circular zone after overnight incubation of antibody with antigen. the specificity and sensitivity of the radial immunodiffu ... | 1984 | 6093644 |
| intranasal vaccination of pigs against pseudorabies: absence of vaccinal virus latency and failure to prevent latency of virulent virus. | the avirulent bartha's k strain of pseudorabies virus (prv) was used to vaccinate 8 pigs at 10 weeks of age by the intransal route (experiment 1). on postvaccination days (pvd) 63 and 91, pigs were treated with corticosteroids. viral shedding could not be detected. explant cultures of trigeminal ganglia and tonsils did not produce virus. four pigs with maternal antibody were vaccinated intranasally with bartha's (attenuated) k strain of prv at 10 weeks of age and were challenge exposed with a vi ... | 1984 | 6093645 |
| natural cytotoxicity detected in swine using aujeszky's disease virus infected targets. | using percoll gradients and standard b and t cell depletion techniques, porcine lymphocytes were investigated for their ability to show natural killing (nk) of many targets, including aujeszky's disease virus (ajdv) infected cells. although it was possible to enrich for nk activity using these techniques it was not possible to define a distinct subpopulation of nk cells. it was noted that porcine nk cells consistently showed a preferential lysis of ajdv infected cells in comparison to uninfected ... | 1984 | 6095387 |
| cytotoxic lymphocytes induced by african swine fever infection. | the generation of lymphocytes cytotoxic to african swine fever virus infected testis cells during in vivo infection is described. peripheral blood lymphocytes from 16 pigs developed cytotoxicity seven to eight days after infection but the lysis was not restricted to autologous cells. | 1984 | 6095388 |
| pathogenicity of a modified-live pseudorabies vaccine virus in lambs. | subcutaneous injections of modified-live pseudorabies virus (prv) vaccine into lambs caused clinical signs and death within 1 week after injection in 4 of 5 inoculated lambs. the clinical signs included depression, high fever, muscle fasciculations and convulsions, occasionally followed by death within 48 hours of the initial clinical signs. histologic examinations and virus isolation procedures demonstrated prv in the cns of infected lambs. sera from sick lambs remained negative for prv antibod ... | 1984 | 6096336 |
| parenteral and intranasal vaccination of fattening pigs against aujeszky's disease (pseudorabies). | 1984 | 6097071 | |
| an electron microscopic study of solar ganglia in piglets infected with aujeszky's disease virus. | ten 2-day-old piglets were inoculated intranasally with aujeszky's disease virus and their solar ganglia were examined by light and electron microscopy. the cytopathic change noticed was neuronal and glial cell degeneration. it was invariably associated with the virus replicated within the cells. all the main stages of virus development were observed in the degenerated ganglionic cells. characteristically, plexiform vermicellar array and electron-dense tubular filaments were detected in a part o ... | 1983 | 6097822 |
| specificity of pseudorabies virus serotests. | pigs experimentally inoculated with bovine herpesvirus-1 or equine herpesvirus-1 developed mild clinical disease signs. regression of clinical disease was accompanied by development of specific virus-neutralizing antibodies. these antibodies did not react positively with pseudorabies antigens in the serum-virus neutralization test, an indirect radioimmunoassay, or a microimmunodiffusion test. | 1984 | 6098206 |
| demonstration of pseudorabies viral antigen in thick and ultrathin tissue sections of young pigs using an immunogold method. | pseudorabies (aujeszky's) virus antigens were labeled in thick and ultrathin tissue sections of young pig brain and liver tissue, using an indirect immunogold method. antigens were tagged with 20 nm gold particles. controls proved the specificity of the reaction in paraffin sections and ultrathin epoxy sections. immunogold staining was compared with immunoperoxidase staining in paraffin sections. in ultrathin sections stained with the immunogold method, the gold particles were present on viral n ... | 1984 | 6208821 |
| [aujeszky's disease: vaccination problems and vaccination advice]. | 1980 | 6243807 | |
| [serological investigations for detection of neutralising antibodies against the virus of aujeszky's disease in pedigree breeding - and piglet producing farms in the district of the animal health office in the chamber of agriculture weser-ems (author's transl)]. | 1980 | 6249563 | |
| [the rotating aeration (system fuchs) for treatment of liquid animal and municipal wastes. 9. report: the effect of aerobic treatment of pig slurry containing pseudorabies virus]. | 1980 | 6250530 | |
| [certain properties of the aujeszky live vaccine "dessau" with special reference to laboratory diagnostic aspects]. | aujeszky live vaccine "dessau" proved to be apathogenetic to and unconditionally tolerable by pigs of all age groups, including pregnant sows. transmission of herpes virus suis vaccine strain from immunised individuals to non-immunised contact animals was not observed at all. higher titres of virus-neutralising serum antibody with longer persistence were not attained until repeated vaccinations had been applied, using aujeszky live vaccine "dessau". spherical detachment of single cells was the o ... | 1980 | 6251770 |
| detection of pseudorabies virus antibodies: time of appearance by two serotests. | this report describes a time-course comparison of detection of pseudorabies virus antibodies in experimentally infected swine by the virus-neutralization (vn) and indirect hemagglutination tests. specific antibody titers were observed by the iha test at 5 days after swine were inoculated, but not until 12 days by the vn test. the predominant immunoglobulin (ig) class present in the serums of the swine at 5 and 7 days after inoculation was igm, as determined by sulfhydryl reductions. the vn test ... | 1980 | 6255835 |
| [vaccination in aujeszky's disease on enzootic fattening farms]. | an experiment was carried out on 125 pigs, originating from immune mothers on an enzootic for a. d. swine-breeding farm. the pigs were vaccinated once and twice with living vaccine mk-25 at different moments after their weaning. on the 180th after the vaccination the pigs were infected with a virulent (10(6) tcid) pseudorabies virus. they were studied clinically, serologically, virologically and pathomorphologically. it was proved that the vaccination of pigs, born by immune mothers, did not bri ... | 1980 | 6259802 |
| [aujeszky disease in pigs: practicability of an elisa for detection of serum antibodies (author's transl)]. | 1981 | 6262038 | |
| [hematological studies of pigs vaccinated against aujeszky's disease]. | hematological investigations within a period of six months were carried out on 4 groups of pigs vaccinated with different doses of living aujeszky mk-25 vaccine. the pigs from groups i and ii were vaccinated with a normal dose of 2 cm3, the pigs from group iii--with 10 doses, whereas those from group iv--with a hundredfold dose. a clearly pronounced immunohemolytic anemia was proved, accompanied by leucocytopenia with an increased percentage of segmental-nuclear leucocytes and monocytes during t ... | 1980 | 6262996 |
| [serological studies on the prevalence of neutralizing antibodies against pseudorabies virus in pigs in hessen (author's transl)]. | 1981 | 6263571 | |
| [immunohistologic antigen demonstration and virus isolation in cell cultures in aujeszky disease]. | 1981 | 6264912 | |
| [the effect of vaccination on the intrauterine transmission of aujeszky's disease virus in sows]. | eight of ten sows of the large white breed were vaccinated in different stages of pregnancy, with an inactivated vaccine against aujeszky's disease. the other two sows remained untreated. two virulent strains of the aujeszky's disease virus were used for the infection of all sows, either orally or intravenously. neither of the two ways of infection led to the transmission of the virus to the foetus in any of the eight vaccinated sows. on the other hand, in the two unvaccinated sows, the virus wa ... | 1981 | 6266115 |
| [comparison of 2 inactivated vaccines against aujeszky's disease in pigs]. | equal efficiency was proved by comparing two inactivated vaccines against aujeszky's disease (ad) virus manufactured in czechoslovakia. fifteen porkers at the age of four weeks were included in the experiment, not possessing any specific antibodies to ad virus. each vaccine was inoculated intramuscularly to five porkers (2 ml) in the interval of three weeks. the third group of five porkers was control. the samples of blood serum were subjected to the serum neutralizing test and radioimmunoassay ... | 1981 | 6267763 |
| [comparative study of the antiviral activity of pancreatic and microbial rnase]. | the efficacy of pancreatic rnase with microbial enzymes (rn-ases) of act. rimosus and bacillus intermedius) was studied comparatively in vitro in a transplantable cell culture of the swine embryokidney with respect to the aphthosa virus (av) and the virus of the aujeszky disease (vad). the vad proved to be most sensitive to rnases. rnase of bac. intermedius showed the highest antiviral efficacy. the enzymes were active in vivo, when the albino mice and newborn rabbits were infected with the av, ... | 1981 | 6267996 |
| induced latency in pseudorabies vaccinated pigs. | a latent pseudorabies virus infection was established in pigs despite vaccination with a modified-live pseudorabies virus vaccine. although the vaccinated pigs developed high concentrations of antibody, virus was recovered from the tonsils and lungs of pigs treated with dexamethasone three months after inoculation with virulent virus. these results may explain why vaccination programs have failed to eliminate the persistence and spread of virulent pseudorabies virus in infected herds. | 1981 | 6268266 |
| comparison of the effect of trisodium phosphonoformate on the mean plaque size of pseudorabies virus, infectious bovine rhinotracheitis virus and pigeon herpesvirus. | 1980 | 6268670 | |
| [aujeszky's disease: serological responsiveness after vaccination of 6-10-week-old piglets with maternal antibody (author's transl)]. | trials were made to study the extent to which 6-10-week-old piglets with and without virus-neutralizing (vn) antibodies are capable of a serological response to vaccination. the piglets were inoculated with three different vaccines: two live vaccines (aescovac aujeszky and mk25 vaccine) and an inactivated vaccine (geskyvac). the titre of virus-neutralizing antibody in the blood was determined for each vaccine in approximately 200 piglets at the time of inoculation as well as within four and twel ... | 1981 | 6269250 |
| [aujeszky's disease: maternal immunity of piglets born of sows inoculated with various vaccines (author's transl)]. | studies on the maternal state of immunity were done in 4-10 week-old piglets born of vaccinated sows free from aujeszky's disease. the sows were inoculated with three different vaccines: two live vaccines (aescovac aujeszky) and the mk25 vaccine) and an inactivated vaccine (geskyvac). the maternal state of immunity of the piglets was assessed from the titre of the virus-neutralizing (vn) antibodies in the blood. to do so, samples were taken from 300-400 4-10-week-old piglets per vaccine inoculat ... | 1981 | 6269251 |
| laboratory evaluation of selected disinfectants as virucidal agents against porcine parvovirus, pseudorabies virus, and transmissible gastroenteritis virus. | of a variety of disinfectants evaluated, only sodium hypochlorite and sodium hydroxide inactivated porcine parvovirus (ppv) after a 5-minute incubation period. after the same incubation time, pseudorabies and transmissible gastroenteritis viruses were inactivated by all of the disinfectants tested. when the incubation time was increased to 20 minutes, 2% glutaraldehyde and a double-strength concentration of a commercial formaldehyde preparation also inactivated ppv. formaldehyde vapor and ultrav ... | 1981 | 6269467 |