Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| comparison of a direct radioimmunoassay with virus isolation in cell culture for detection of pseudorabies virus in tissues of infected swine. | a direct radioimmunoassay (dria) was developed for detection of pseudorabies virus (prv) in tissues of pigs with clinical disease. homogenates of tissues prepared from 289 swine were tested for prv with the dria and virus-isolation technique (vit). the virus was detected in 53 (18%) of the homogenates by both dria and vit. fifteen (5%) others were positive for prv by vit, but negative by dria. there were no samples negative by vit and positive by dria. a total of 221 (77%) pigs with clinical prv ... | 1982 | 6280535 |
| pseudorabies virus infection in raccoons: a review. | pseudorabies is a rarely reported disease of raccoons. laboratory and field evidence of prv infection suggests the raccoon is a "dead end" host with little opportunity for raccoon-to-raccoon spread of virus. all reported field cases have been associated closely with infected swine and swine have been considered the source of the raccoon infection. the clinical signs of prv in raccoons closely resembles those of canine distemper and rabies virus infections. infection with the latter viruses are c ... | 1982 | 6285008 |
| oronasal challenge of fattening pigs after vaccination with an inactivated aujeszky's disease vaccine. | groups of pigs from vaccinated and unvaccinated sows were vaccinated once or twice between the ages of eight and 20 weeks with a commercial inactivated, oil adjuvanted aujeszky's disease virus vaccine. pigs were challenged by the oronasal route when 22 to 27 weeks old. pigs from unvaccinated sows developed neutralising antibodies after vaccination but no seroconversion was detected in eight-week-old pigs or in 80 per cent of 15-week-old pigs from vaccinated sows. challenge resulted in severe dis ... | 1982 | 6283610 |
| isoelectric focusing of infectious particles of porcine pseudorabies virus strains in granulated dextran gels. | isoelectric focusing of infectious particles of four strains of porcine pseudorabies viruses (indiana, iowa, shope and an avirulent live-virus vaccine strains) are described. the pseudorabies virus strains exhibited great mobility in the electric field typical of viruses of the herpes group. strains were considered electrophoretically homogeneous based on their respective isoelectric points. maximal virus infectivity was concentrated in reproducible, stationary zones representing 73 to 86% of th ... | 1982 | 6280822 |
| the incidence of aujeszky's disease in finland. | 1982 | 6294595 | |
| shedding patterns in swine of virulent and attenuated pseudorabies virus. | shedding patterns of 2 virulent (p-2208 and kc-152-d) and 1 attenuated (buk) strains of pseudorabies virus (prv) were determined in groups of intranasally inoculated feeder pigs. clinical signs observed following inoculation with the p-2208 and kc-152-d strains included increase in rectal temperatures up to 42.2 c, anorexia, severe respiratory disturbance, and fatal cns signs in 2 cases. clinical signs in pigs inoculated with 7.2 x 10(7) median tissue culture infective dose (tcid50) of the buk s ... | 1983 | 6316822 |
| [effect of vaccination against aujeszky's disease on the physical development and fertility of boars]. | 1983 | 6317331 | |
| [aujeszky's disease in swine]. | 1983 | 6314593 | |
| occurrence and reactivation of latent aujeszky's disease virus following challenge in previously vaccinated pigs. | pigs which were vaccinated with an inactivated adv vaccine developed a latent adv infection up to 18 months after adv challenge. up to 6.5 months p.i. latent virus could be demonstrated by co-cultivation of different organ tissues (lungs, tonsils, olfactory bulb, brain stem, medulla). afterwards, reactivation of latent virus was only achieved by immunosuppression of the animals. immunosuppression led to a limited virus replication in nasal mucosa, tonsils, lymph nodes and central nervous system. ... | 1983 | 6299245 |
| evaluation in swine of a subunit vaccine against pseudorabies. | a subunit vaccine against pseudorabies virus (prv) was prepared by treating a mixture of pelleted virions and infected cells with the nonionic detergent nonidet p-40 and emulsifying the extracted proteins incomplete freund's adjuvant. three 7-week-old pigs without antibodies against prv were given 2 im doses of this vaccine 3 weeks apart. thirty days after the 2nd vaccination, 10(6) median tissue culture infective doses (tcid50) of a virulent strain of prv were administered intranasally. tonsill ... | 1983 | 6297337 |
| pseudorabies virus nucleocapsid antigen for skin testing in swine. | a procedure is described for the production of an effective pseudorabies virus skin test antigen consisting of nucleocapsids. the nucleocapsids were prepared by disruption of pseudorabies virus-infected pig kidney cells and nonionic detergent removal of the viral envelope. this preparation was noninfectious for pig kidney cell cultures and mice. swine that had recovered from a pseudorabies virus infection and were injected intradermally with the nucleocapsid antigen developed a delayed type hype ... | 1983 | 6302131 |
| a study in calves, swine and rabbits of the immunological relationships between infectious bovine rhinotracheitis virus and aujeszky's disease virus. | 1983 | 6306958 | |
| pseudorabies virus, porcine parvovirus, and porcine enterovirus interactions with the zona pellucida of the porcine embryo. | porcine embryos (n = 93) were incubated on cell monolayers that had been previously inoculated with pseudorabies virus, porcine parvovirus (ppv), or each of 2 porcine enteroviruses. after 2, 24, or 48 hours of incubation, the embryos were fixed in glutaraldehyde and examined by electron microscopic procedures. it was found that pseudorabies virus adsorbed to the zona pellucida (zp) and entered sperm tracks in the zp. the ppv and both enteroviruses entered pores in the zp and were associated with ... | 1983 | 6307093 |
| comparison and evaluation of four serological tests for detection of antibodies to aujeszky's disease virus. | four methods used for the detection of antibodies to aujeszky's disease virus in porcine sera were compared. the serum neutralisation test, countercurrent immunoelectrophoresis test, micro-immunodiffusion test and the enzyme-linked immunosorbent assay (elisa) were assessed with particular regard to their use in large scale screening of porcine serum samples. the elisa test was found to be the most suitable on the grounds of sensitivity, speed and cost. | 1983 | 6310845 |
| [maternal antibodies against aujeszky-virus in piglets of vaccinated sows]. | 1983 | 6299685 | |
| development of a nonneuronal cell line derived from porcine spinal ganglia. | a cell line from porcine spinal ganglion (pspg) tissue was initiated and characterized. the pspg cell line contained epithelioid, fibroblast-like, and stellate-like cells. there were numerous intercellular communications between cells in the culture. neuronal cells were not present in the pspg cell line. the pspg cell line was aneuploid at the 5th and 45th subculture levels. seven porcine viruses replicated in cultures of pspg cells, and 2 pseudorabies virus strains infected the cells after bein ... | 1983 | 6402962 |
| [aujeszky's disease]. | 1983 | 6326383 | |
| restriction endonuclease analysis of the pseudorabies (aujeszky's disease) virus before and after serial passage in vivo and in vitro. | the deoxyribonucleic acid (dna) of pseudorabies virus (prv) was examined by restriction endonuclease analysis before and after various treatments: namely 1. plaque purification of stock virus, 2. serial passage of virus in cell culture at high (h) and low (l) multiplicity of infection (moi), and 3. serial passage of virus in swine. the objective of the study was to determine if such treatments would either select or induce virus populations with a different predominant number or location of enzy ... | 1983 | 6318695 |
| the effects of challenge on the humoral and cellular immune responses in pseudorabies vaccinated swine. | the effects of challenge exposure on the humoral and cellular immune responses in pseudorabies vaccinated swine were studied in 84 barrows. the pigs were divided into seven groups and challenge exposed to a virulent strain of pseudorabies virus on months 1, 3, 5, 8, 10, 12 and 14 after vaccination. the pigs were vaccinated with commercial attenuated and inactivated pseudorabies virus vaccines. the protection conferred by vaccination was equally effective with both types of vaccines. the levels o ... | 1983 | 6321001 |
| evaluation of the microimmunodiffusion test for the detection of antibody to pseudorabies virus. | 1983 | 6297340 | |
| experimental aujeszky's disease in pigs: excretion, survival and transmission of the virus. | airborne aujeszky's disease virus was recovered from looseboxes containing groups of pigs infected with virus strains from england, northern ireland and denmark from days 1 to 7 after infection. pigs sampled individually excreted most airborne virus on days 2 and 3 after infection. on a 24 hour basis the maximum amount of airborne virus excreted per pig was log10 5.3 tcid50. subclinical infection was transmitted from a clinically affected group of pigs to a seronegative group held in separate lo ... | 1983 | 6316624 |
| effects of vaccination on lesion development in pseudorabies virus-challenged swine. | the effects of vaccination against pseudorabies virus (prv) infection were studied in 93 barrows. commercial attenuated and inactivated prv vaccines were used. the protection provided was evaluated by the comparison of clinical signs, gross lesions, and microscopic lesions among vaccinated and nonvaccinated pigs after challenge exposure with a sublethal dose of prv. histopathologic changes are described for the cns and other tissues. neither the modified live- nor the inactivated-prv vaccines pr ... | 1983 | 6307084 |
| embryo transfer for conserving valuable genetic material from swine herds with pseudorabies. | embryo transfer was used to conserve genetic material from 2 swine herds seropositive for pseudorabies virus (prv). embryos (n = 805) were recovered from 38 prv-seropositive duroc sows in iowa and, after 4 to 10 hours' culture and shipment to illinois, were transferred to 34 recipients from a herd seronegative for prv. all recipients remained seronegative for prv, and 22 of the recipients farrowed 208 pigs (189 alive) that also were seronegative for prv. there was no evidence of prv in the embry ... | 1983 | 6311780 |
| serological response of pigs infected with aujeszky's disease virus. | the temporal development of antibody in four groups of pigs infected with different aujeszky's disease virus isolates was examined. the enzyme-linked immunosorbent assay detected antibody by five to six days after infection and the antibody-dependent cell-mediated cytotoxicity assay detected antibody seven to nine days after infection. neutralising antibody was first detected nine to 10 days after infection, whereas assays measuring complement mediated antibody lysis did not detect antibody unti ... | 1983 | 6314461 |
| serologic survey of california wild hogs for antibodies against selected zoonotic disease agents. | blood samples were collected from trapped or hunter-killed wild hogs (sus scrofa) in 4 areas of california. sera were tested for antibodies against 7 zoonotic disease agents. antibodies against brucella sp were detected in 21 (15%) of 136 samples. antibodies against coxiella burnetii were found in 50% of the collected samples (67 of 135 tested). of the 135 wild hogs screened for pseudorabies virus, 4 (3%) were seropositive. leptospira interrogans antibodies were discovered in 118 (87%) of the 13 ... | 1983 | 6315659 |
| pseudorabies: experimental studies in raccoons with different virus strains. | raccoons (procyon lotor) were infected by the nasal/oral route with as little as 10(2) plaque forming units (pfu) of pseudorabies virus (prv). there was no apparent difference in the susceptibility of raccoons to infection with either of two virulent field strains or with the naturally avirulent k strain which has been used in modified live virus vaccines. each of these three viruses was transmitted by contact to uninfected raccoons. all raccoons that were infected with virulent field strains di ... | 1983 | 6315973 |
| [use of solid-phase radioimmunoassay (ria) for the determination of antibodies to aujeszky's disease virus in the blood serum of vaccinated pigs]. | the specific antibodies to aujeszky's disease virus were determined by the method of solid-phase radioimmunoassay (ria) in the blood serums of pigs vaccinated with inactivated vaccine from three different producers. as found, in some groups of vaccinated pigs the results of ria examination are adversely affected by the relationship of antibodies to cellular antigenic determinants. this demonstrates that, after vaccination, antibodies are produced not only against the virus antigen but also again ... | 1983 | 6322405 |
| an electron microscopic study of solar ganglia in piglets infected with aujeszky's disease virus. | ten 2-day-old piglets were inoculated intranasally with aujeszky's disease virus and their solar ganglia were examined by light and electron microscopy. the cytopathic change noticed was neuronal and glial cell degeneration. it was invariably associated with the virus replicated within the cells. all the main stages of virus development were observed in the degenerated ganglionic cells. characteristically, plexiform vermicellar array and electron-dense tubular filaments were detected in a part o ... | 1983 | 6097822 |
| specificity of pseudorabies virus serotests. | pigs experimentally inoculated with bovine herpesvirus-1 or equine herpesvirus-1 developed mild clinical disease signs. regression of clinical disease was accompanied by development of specific virus-neutralizing antibodies. these antibodies did not react positively with pseudorabies antigens in the serum-virus neutralization test, an indirect radioimmunoassay, or a microimmunodiffusion test. | 1984 | 6098206 |
| demonstration of pseudorabies viral antigen in thick and ultrathin tissue sections of young pigs using an immunogold method. | pseudorabies (aujeszky's) virus antigens were labeled in thick and ultrathin tissue sections of young pig brain and liver tissue, using an indirect immunogold method. antigens were tagged with 20 nm gold particles. controls proved the specificity of the reaction in paraffin sections and ultrathin epoxy sections. immunogold staining was compared with immunoperoxidase staining in paraffin sections. in ultrathin sections stained with the immunogold method, the gold particles were present on viral n ... | 1984 | 6208821 |
| [epidemiology of aujeszky's disease in switzerland]. | 1984 | 6091267 | |
| vaccination against aujeszky's disease: field experiences. | twelve herds were investigated where outbreaks of clinical aujeszky's disease had occurred. clinical aujeszky's disease was eliminated from all farms following vaccination. after vaccination was stopped in two of the six fattening herds virus was also apparently eradicated, judging from serological examination. these two herds were the smallest of the six fattening farms and size and the throughput of pigs may have contributed to apparent eradication of virus. in two of six breeding farms where ... | 1984 | 6093329 |
| evaluation of field isolates of pseudorabies (aujeszky's disease) virus as determined by restriction endonuclease analysis and hybridization. | sixty-seven isolates of pseudorabies virus (prv) from 13 states were cleaved with 4 restriction endonucleases (re), and after electrophoresis in agarose, their banding patterns were photographed and evaluated. the deoxyribonucleic acid (dna) cleavage fragments were designated into regions specified by molecular weight ranges based on lambda phage dna as a size marker. the 67 prv isolates were evaluated according to the total number of cleavage fragments, by the number of fragments within designa ... | 1984 | 6093637 |
| natural cytotoxicity detected in swine using aujeszky's disease virus infected targets. | using percoll gradients and standard b and t cell depletion techniques, porcine lymphocytes were investigated for their ability to show natural killing (nk) of many targets, including aujeszky's disease virus (ajdv) infected cells. although it was possible to enrich for nk activity using these techniques it was not possible to define a distinct subpopulation of nk cells. it was noted that porcine nk cells consistently showed a preferential lysis of ajdv infected cells in comparison to uninfected ... | 1984 | 6095387 |
| cytotoxic lymphocytes induced by african swine fever infection. | the generation of lymphocytes cytotoxic to african swine fever virus infected testis cells during in vivo infection is described. peripheral blood lymphocytes from 16 pigs developed cytotoxicity seven to eight days after infection but the lysis was not restricted to autologous cells. | 1984 | 6095388 |
| some characteristics of four attenuated vaccine virus strains and a virulent strain of aujeszky's disease virus. | in the present study four attenuated virus strains, used as vaccines, and a virulent strain of aujeszky's disease virus (adv) were compared with respect to their virulence in mice, their ability to induce virus-specified thymidine kinase (tk) in infected cells, and their cleavage profiles of viral dna's after treatment with the restriction endonuclease kpni. the survival time of mice inoculated with the b-kal or the virulent nia-3 strain was comparable, whereas the bartha and buk strains require ... | 1984 | 6093331 |
| pathogenicity of a modified-live pseudorabies vaccine virus in lambs. | subcutaneous injections of modified-live pseudorabies virus (prv) vaccine into lambs caused clinical signs and death within 1 week after injection in 4 of 5 inoculated lambs. the clinical signs included depression, high fever, muscle fasciculations and convulsions, occasionally followed by death within 48 hours of the initial clinical signs. histologic examinations and virus isolation procedures demonstrated prv in the cns of infected lambs. sera from sick lambs remained negative for prv antibod ... | 1984 | 6096336 |
| swine diseases transmissible with artificial insemination. | the transport of fresh and frozen semen to be used for artificial insemination creates a mode of disease transmission between farms. normally, semen contains a number of nonpathogenic bacterial contaminants; however, excessive bacterial contamination can result in infertile matings. contamination with a known pathogen, eg, brucella suis, could initiate a serious outbreak of disease in a recipient herd. methods to minimize bacterial contamination of semen include sanitary collecting and processin ... | 1984 | 6090372 |
| genetic basis of the neurovirulence of pseudorabies virus. | lomniczi et al. (j. virol. 49:970-979, 1984) have shown previously that two attenuated vaccine strains of pseudorabies virus have a similar deletion in the short unique (us) region of the genome. the region which is deleted normally codes for several translationally competent mrnas. as expected, these mrnas are not formed in the cells infected with the vaccine strains. the function specified by these mrnas is thus not necessary for growth in cell culture. using intracerebral inoculation of 1-day ... | 1984 | 6090697 |
| [resistance of aujeszky disease viruses, present in the blood of pigs, to potassium thiosulphate and hydrogen chloride]. | a trial was conducted to study the sensitivity of the aujeszky disease virus, present in the blood of pigs, to the effect of two preservatives: potassium thiosulphate ( k2s2o5 ) and hydrogen chloride (hcl). the sensitivity of the virus was determined on rabbits infected with pig blood containing aujeszky disease virus with different additions of the two preservatives. as found, 0.5% of k2s2o5 and 3% of hcl are concentrations fully sufficient for the inactivation of all the viruses present in the ... | 1984 | 6087536 |
| testicular changes observed in boars following experimental inoculation with pseudorabies (aujeszky's) virus. | four boars were inoculated intranasally with pseudorabies virus to determine if microscopic testicular changes occurred as a result of infection. testicular biopsies and semen samples were taken at two, four and six weeks postinoculation and the boars were castrated immediately after the last sample collection. testicular samples and semen were cultured to determine if the virus was present. pseudorabies virus was not isolated from the semen or testicular tissue. virus was isolated from trigemin ... | 1984 | 6089983 |
| construction of e. coli expression plasmid libraries: localization of a pseudorabies virus glycoprotein gene. | we describe the use of a combined cloning/expression protocol to identify a gene encoding a pseudorabies virus (prv) glycoprotein. prior to this study, the genome locations of prv glycoproteins had not been described. we first identified prv glycoproteins using antibodies directed against prv virions. using affinity chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the prv glycoproteins were separated and antibodies were made against them in rabbits. using dnase diges ... | 1984 | 6090566 |
| parenteral and intranasal vaccination of fattening pigs against aujeszky's disease (pseudorabies). | 1984 | 6097071 | |
| stability of the pseudorabies virus genome after in vivo serial passage. | restriction endonuclease patterns of pseudorabies virus (prv) dna were examined after each of 11 serial passages of the virus through pigs. minor variations in the electrophoretic mobility of certain restriction enzyme fragments were observed by the sixth passage. this variability was similar to some of the minor variability observed in field isolates. the variable fragments were mapped to three locations on the prv genome: the junction between the short unique sequences and the repeat sequences ... | 1984 | 6086823 |
| influence of vaccination on aujeszky's disease virus and disease transmission. | parenteral vaccination of fattening pigs with either modified live or inactivated aujeszky's disease virus did not prevent infection with field strain virus or the development of clinical disease. the duration and severity of the clinical syndrome was, however, reduced and vaccinated pigs did not suffer the severe weight loss and high mortality experienced by non-vaccinated pigs in the acute phase of disease. the range of tissues in which challenge virus replication took place was more restricte ... | 1984 | 6089404 |
| comparison of a modified counterimmunoelectrophoresis test and a microimmunodiffusion test for detection of pseudorabies virus antibodies in porcine sera. | the correlation of a modified counterimmunoelectrophoresis (cie) test and a microimmunodiffusion test for detecting pseudorabies virus antibodies in porcine sera was investigated, using as reference a standard virus neutralization test. the counterimmunoelectrophoresis test exhibited a sensitivity comparable to the microimmunodiffusion test but was not as sensitive as the virus neutralization test. the best feature of the modified counterimmunoelectrophoresis test is that it is a rapid test. it ... | 1984 | 6324974 |
| [vaccination of boars against aujeszky's disease. 2. virus excretion in nasal secretions and ejaculates]. | 1984 | 6325113 | |
| the evaluation of a lectin-agarose based subunit vaccine and complementary diagnostic antigen for aujeszky's disease (pseudorabies) in the pig. | eleven out of 25 pigs were immunized with a lectin--agarose based subunit vaccine for aujeszky's disease (ad). the vaccine was prepared by extracting protective antigens from a non-ionic detergent (triton-x-100) extract of ad virus-infected pk-la cells with lens culinaris agglutinin immobilized on agarose beads. two groups of 3 and 4 pigs received 2 doses of vaccine each containing 426 micrograms of adsorbed protein. two groups of 2 pigs each received 2 vaccine doses containing either 23 or 33 m ... | 1984 | 6326377 |
| necrotizing enteritis in piglets associated with aujeszky's disease virus infection. | 1984 | 6464305 | |
| immune response of sows and their offspring to pseudorabies virus: serum neutralization response to vaccination and field virus challenge. | one month prior to breeding, sows were vaccinated with an attenuated pseudorabies virus vaccine or challenged with a field strain of pseudorabies virus. a third group of sows were not vaccinated or challenged before breeding. pigs from these sows were vaccinated at 3, 6, or 12 weeks of age and challenged with virulent virus three weeks later. one pig from each litter served as an unvaccinated, unchallenged control. serum neutralization titers of these pigs were monitored from birth until 22 week ... | 1984 | 6326982 |
| the effect of pseudorabies (aujeszky's) virus infection on young mature boars and boar fertility. | this study was designed to determine the effects of experimental inoculation with pseudorabies virus on the reproductive tracts of young adult boars. pseudorabies virus was inoculated intranasally into 12 boars and intrapreputially into four boars. all animals seroconverted after nasal or preputial inoculation. semen abnormalities were observed 21 days postinoculation with partial recovery by 50 days postinoculation. virus was isolated from the preputial sheath of two intrapreputially inoculated ... | 1984 | 6326983 |
| results of field trials with a pseudorabies virion skin test antigen. | 1984 | 6327588 | |
| confirmation of pseudorabies virus infection, using virus recrudescence by dexamethasone treatment and in vitro lymphocyte stimulation. | a corticosteroid treatment regimen, together with peripheral blood lymphocyte transformation and virus isolation, were used as epizootiologic tools to study pigs seropositive to pseudorabies (pr) virus, but of unknown pr viral infection status. after treatment with 160 mg of dexamethasone daily for 5 days, pr virus was isolated from 2 of 3 adult littermate boars that carried antibodies to pr virus. these pigs also transferred infection to a specific-pathogen-free sentinel penmate . after dexamet ... | 1984 | 6329047 |
| genetic differences in serum-neutralization titers of pigs after vaccination with pseudorabies modified live-virus vaccine. | four-week-old pigs of the chester white, duroc, hampshire, landrace, and yorkshire breeds (n = 518) were vaccinated with a pseudorabies modified live-virus vaccine to determine whether genetic differences existed for immune response after vaccination. all pigs and their dams (sows) were tested before vaccination to determine preimmunization antibody titers, using a microtitration serum-neutralization (sn) test. the sn test results of sows were negative, as were preimmunization tests of the pigs. ... | 1984 | 6331234 |
| use of hyperimmune serum, vaccination, and certain management procedures for control of pseudorabies in swine. | a program involving use of hyperimmune serum, vaccination, and certain management procedures for the control of pseudorabies was evaluated on several farms on which the disease was enzootic. neonatal mortality and reproductive failures on farms utilizing the program were compared with death losses and reproductive failures on farms not utilizing the program. the reduction in preweaning mortality and reproductive failures between the treated and nontreated groups were 42.1% and 39.6%, respectivel ... | 1984 | 6330015 |
| necrotizing vasculitis in piglets infected orally with the virus of aujeszky's disease. | 1984 | 6330418 | |
| [persistence of virus in swine stock and breeding farms following an outbreak of aujeszky's disease]. | on seven fattening farms and seven breeding farms, investigations were carried out to examine whether aujeszky virus was capable of persisting in particular animal groups or units of the farm as a productive infection following a virologically verified outbreak. after an outbreak on fattening farms, virus persisted temporarily as newly arrived piglets became systematically infected. on the farms on which this could be accurately followed, virus persistence continued for at least 1 1/2 to 2 month ... | 1984 | 6330930 |
| [immunizing properties of 3 vaccines against aujeszky's disease]. | 1984 | 6331335 | |
| tonsillar changes in pigs given pseudorabies (aujeszky's disease) virus. | all of the eight 5-day-old pigs orally given pseudorabies (aujeszky's disease) virus developed tonsillitis. the initial changes occurred in the subepithelial area between the lymphoid nodule and the crypt epithelium, showing a characteristic pattern of necrosis. the necrosis became more severe and gained access into the lymphoid nodule and crypt epithelium. coincident with the histopathologic changes, numerous specific immunofluorescences were detected, first in the nucleus and in some parts of ... | 1984 | 6370049 |
| radial immunodiffusion enzyme assay for detection of antibodies to pseudorabies virus in swine serum. | a radial immunodiffusion enzyme assay for the detection and quantitation of antibodies to pseudorabies virus in swine sera was developed and the methods were standardized. the assay combined the principle of radial immunodiffusion with enzyme-linked immunosorbent assay. quantitation of pseudorabies virus antibody titers was determined by measuring the diameter of a colored circular zone after overnight incubation of antibody with antigen. the specificity and sensitivity of the radial immunodiffu ... | 1984 | 6093644 |
| in vivo and in vitro reactivation of latent pseudorabies virus in pigs born to vaccinated sows. | latency of pseudorabies virus (prv) was established in 8 of 9 pigs born to 2 vaccinated sows. pigs had high, low, or no maternal antibody titers at the time of the initial inoculation. at postinoculation months 3 to 4, latent prv could be reactivated in vivo by the administration of large doses of corticosteroids. in most pigs, the stress-simulating treatment resulted in recrudescence of virus shedding after lag periods of 4 to 11 days. in 3 pigs, virus shedding was without clinical signs of dis ... | 1984 | 6324622 |
| rapid diagnosis of aujeszky's disease in pigs by immunofluorescence. | direct immunofluorescence on impression smears of brain and pharynx was compared with virus isolation in cell culture for the diagnosis of aujeszky's disease in experimentally and naturally infected pigs. pharyngeal impression smears were more sensitive than virus isolation in two pigs killed 10 and 12 days after experimental infection. both methods were of similar sensitivity in the detection of virus from field cases of disease. smears of brain and pharynx were more sensitive than virus isolat ... | 1984 | 6326215 |
| intranasal vaccination of pigs against pseudorabies: absence of vaccinal virus latency and failure to prevent latency of virulent virus. | the avirulent bartha's k strain of pseudorabies virus (prv) was used to vaccinate 8 pigs at 10 weeks of age by the intransal route (experiment 1). on postvaccination days (pvd) 63 and 91, pigs were treated with corticosteroids. viral shedding could not be detected. explant cultures of trigeminal ganglia and tonsils did not produce virus. four pigs with maternal antibody were vaccinated intranasally with bartha's (attenuated) k strain of prv at 10 weeks of age and were challenge exposed with a vi ... | 1984 | 6093645 |
| eradication of some infectious pig diseases by perinatal tiamulin treatment and early weaning. | from 10 days before the expected date of farrowing onwards, 97 sows infected by mycoplasma hyopneumoniae and treponema hyodysenteriae were given tiamulin daily at a dosage of 20 mg/kg bodyweight via the feed. three days before farrowing the sows were washed with a disinfectant and transferred to an isolated farrowing house. the sucking piglets remained with their dams for five days, during which time the sows continued to receive the tiamulin-containing feed. the sucking piglets also received ti ... | 1985 | 3984171 |
| vaccines against aujeszky's disease: evaluation of their efficacy under standardized laboratory conditions. | a standardized test was developed to compare the efficacy of aujeszky's disease virus (adv) vaccines under laboratory conditions. per test 3 groups of 6 to 8 sero-negative pigs were used. the first vaccination was done at 10 weeks of age. one group was vaccinated once, another was vaccinated twice and the 3rd served as control. pigs were challenge exposed to the virulent nia-3 strain of adv 12 weeks after the first vaccination. apart from mortality, average periods of growth arrest, fever and vi ... | 1985 | 2996210 |
| pseudorabies virus infection in piglets accompanying with the lesion of bilateral encephalomalacia. | 1985 | 3981837 | |
| dna hybridization procedure to detect pseudorabies virus dna in swine tissue. | a dna hybridization technique was developed to detect the presence of pseudorabies virus (prv) dna. p nick translated probes of high specific activity were prepared from transformed escherichia coli plasmids into which bacillus amyloliquefaciens h (bam h1) restriction fragments of prv dna had been inserted. swine cellular dna and tissue culture prv dna were digested with bam h1, separated by agarosegel electrophoresis, transferred onto nitrocellulose paper, hybridized to the radioactive probes, ... | 1985 | 2408522 |
| presence of markers for virulence in the unique short region or repeat region or both of pseudorabies hybrid viruses. | the unique short region and part of the repeat region of virulent pseudorabies virus strain nia-3 was replaced by the corresponding region of the avirulent nia-4 strain by transfection with subgenomic dna fragments. the resulting hybrid virus showed a reduced virulence in both mice and pigs. therefore, important markers for virulence are located in the unique short or repeat region or both of pseudorabies virus. we provide evidence that the terminally located repeat is not required for the gener ... | 1985 | 2981366 |
| [thymidine kinase activity of virulent and vaccinal strains of aujeszky's disease virus]. | studied was the timidine-kinase activity of the a2 virulent strain and the mk-25 and mk-35 vaccinal strains of the aujeszky's disease virus via the infection of primary cell cultures of chick embryo fibroblasts and of pig kidney. it was found that the a2 strain substantially enhanced the induction of the timidine-kinase enzyme in the infected cell cultures, while the iudr- and the budr-resistant vaccinal strains mk-25 and mk-35 showed 10 to 12 times lower activity. strain mk-25 did not restore t ... | 1985 | 2986347 |
| [cell-mediated cytotoxicity and lymphocyte stimulation in aujeszky's disease. i. in experimentally infected swine]. | 1985 | 2986385 | |
| [cell-mediated cytotoxicity and lymphocyte stimulation in aujeszky's disease. ii. following vaccination of swine and subsequent infection]. | 1985 | 2986388 | |
| quantitative evaluation of syncytium formation in cell culture by british isolates of aujeszky's disease virus. | 1985 | 2986757 | |
| [survival of aujeszky's disease virus in infected pig slurry]. | it has been demonstrated that after experimental infection of pig slurry from the space under the slatted floor (infection dose of 10(6)pfu per ml), the aujeszky's disease virus (adv) survived for 72 hours at the temperature of 15 degrees c and at ph 6.5, but was inactivated after 96 hours. when technologically treated pig slurry from the storage tanks was saturated with water and infected with adv at the dose of 10(5)pfu per ml, the virus survived for 23 days when kept at 15 degrees c and 4 deg ... | 1985 | 2992149 |
| intranasal vaccination of pigs against aujeszky's disease. 4. comparison with one or two doses of an inactivated vaccine in pigs with moderate maternal antibody titres. | intranasal (in) vaccination of pigs with low levels of maternally-derived antibody (mda) has previously been shown to confer good protection against challenge with virulent aujeszky's disease virus (adv). the objective of the present study was to determine the efficacy of in vaccination with an attenuated adv, in comparison with that of an inactivated vaccine given parenterally, in pigs with higher mda titres at the time of vaccination. in one experiment, vaccinations were done at 6 weeks of age ... | 1985 | 2996212 |
| the rapid detection of aujeszky's disease virus in pigs by direct immunoperoxidase labelling. | direct immunoperoxidase labelling on impression smears of brain and pharynx was compared with virus isolation and direct immunofluorescence for the detection of aujeszky's disease virus in experimentally-infected pigs. immunoperoxidase labelling was as sensitive as immunofluorescence and more sensitive than virus isolation for tissue that had been stored at room temperature (approximately 20 degrees c) for up to 144 h. | 1985 | 2996214 |
| prenatal and preweaning deaths caused by pseudorabies virus and porcine parvovirus in a swine herd. | sequential outbreaks of pseudorabies virus and porcine parvovirus infections were documented at a swine farm in southern minnesota. data for the prevalence of mummified fetuses born and the preweaning mortality were recorded over a 3-year-period. the farm was a farrow-to-finish facility, with breeding females housed in 4 groups according to their stage of pregnancy. the herd consisted of approximately 130 breeding females in december 1981, and expanded to 220 females during the 12 months of 1982 ... | 1985 | 2997093 |
| relation between the levels of mrna abundance and kinetics of protein synthesis in pseudorabies virus-infected cells. | virus proteins synthesized by pseudorabies virus-infected cells can be classified into five groups on the basis of the kinetics of their synthesis at various stages of the infective process; virus mrnas can similarly be classified into four groups. to determine whether the kinetics of synthesis of specific proteins are determined solely by the level of abundance in the cells of their mrnas, we have compared at various times after infection the relative synthesis of these proteins with the relati ... | 1985 | 2998025 |
| detection of antibodies to aujeszky's disease virus in whole blood by elisadisc. | a rapid method for the detection of antibodies in whole blood by elisa was developed. a punched filter paper disc configuration, the elisadisc, was loaded with blood, dried, and applied directly to the aujeszky's disease elisa, with minimal pretreatment. rapidity, throughput and sensitivity were equal to that of the serum elisa. no loss of activity could be detected in the discs after storage at +4 degrees c for almost 1 yr. | 1985 | 3001121 |
| [the persistence of activity of h1n1 (swine) influenza virus in pig breeding units during non-epidemic phases]. | a study is carried on in a group of 16 commercial breeding-finishing units in brittany (france). the aim of this study is to try to reveal the persistence of activity of the influenza virus within these intensive units after an acute epizootic. in each farm two batches of pigs are selected, individually identified and followed from suckling period to slaughter. serological controls are conducted every month on the same pigs. both influenza and aujeszky's disease antibodies are apprehended. furth ... | 1985 | 3004802 |
| [modification of the aujeszky's virus neutralization test attributable different test conditions]. | 1985 | 2994980 | |
| comparative usefulness of tissue fixatives for in situ viral nucleic acid hybridization. | traditionally tissues for in situ hybridization of viral nucleic acid have been small pieces obtained from laboratory rodents, and fixatives that are designed for electron microscopy, such as periodate-lysine-paraformaldehyde (plp) can handle them adequately. however, these fixatives have limited penetrating ability and may produce no appreciable hardening, so alternative fixation methods were evaluated. the intention was to determine whether fixatives adequate for bulky tissues such as whole or ... | 1985 | 2995481 |
| [simultaneous vaccination against aujeszky's disease and european hog cholera]. | 1985 | 2986592 | |
| evaluation of the radial immunodiffusion enzyme assay for the detection of antibodies to pseudorabies virus. | the validity of radial immunodiffusion enzyme assay (ridea) as a diagnostic test for antibodies to pseudorabies virus (prv) in porcine serum was determined. serum samples from sows and offspring were tested for the presence of antibodies to prv, using both the ridea and the prv serum-neutralization (sn) test. overall sensitivity and specificity of the ridea done on serums from the sows were 95.7% and 95.2%, respectively. this sensitivity compares with 97.3% sensitivity of the sn test of the same ... | 1985 | 2987167 |
| analysis of reactivation of latent pseudorabies virus infection in tonsils and gasserian ganglia of pigs. | multiplication of pseudorabies virus (prv) strain top and establishment of latency were followed in the tonsils and gasserian ganglia (gg) of 8-week-old pigs after oral infection. already within 24 hr, the titre of prv in tonsils reached 10(2) tcid50/g tissue, its presence in the oropharynx being detectable by swabbing. virus multiplication in tonsils culminated on days 3-6 p.i. (10(6)-10(6.5) tcid50/g) and continued till day 11 post-infection (p.i.), when its titre was 10(1.5) tcid50/g the swab ... | 1985 | 2866693 |
| pathologic effects of intrauterine deposition of pseudorabies virus on the reproductive tract of swine in early pregnancy. | pseudorabies virus was inoculated into the uterus of 15 gilts within 6 hours after natural breeding, and gilts were necropsied at postbreeding days (pbd) 3, 6, 10, 14, and 28; 3 control gilts were treated similarly, except for inoculation with pseudorabies virus and were necropsied at pbd 6, 10, and 14. tissues were collected for virus isolation, fluorescent antibody staining, and histopathologic examination. pseudorabies virus was isolated from the reproductive tract up to day 14. lesions in th ... | 1985 | 2988375 |
| intranasal vaccination of pigs against aujeszky's disease: comparison with inactivated vaccines in pigs with low maternal antibody titres. | intranasal vaccination with an attenuated aujeszky's disease virus strain was compared with parenteral vaccination with two inactivated virus vaccines, in 10-week-old pigs with low levels of maternal antibody. intranasal vaccination conferred a much better protection than parenteral vaccination with the two inactivated vaccines against challenge two months later, as evidenced by shorter periods of growth arrest and fever and a greater reduction of virulent virus shedding after challenge-exposure ... | 1985 | 2994188 |
| pseudorabies virus in dexamethasone-treated pigs. | 1985 | 2994277 | |
| vaccination of swine with thymidine kinase-deficient mutants of pseudorabies virus. | a mutant of pseudorabies virus (prv) deficient in thymidine kinase (tk-) activity was isolated and characterized. the mutant grew well in cell culture and did not revert to the thymidine kinase-positive phenotype. the prv-tk- was not virulent when inoculated intranasally into 3-to 4-week-old pigs and could not be reactivated from the ganglia of these pigs by explantation and cocultivation with susceptible cells several weeks after virus inoculation. pigs that had been exposed to prv-tk- were imm ... | 1985 | 2992326 |
| production and characterization of monoclonal antibodies directed against pseudorabies virus. | hybridoma cell lines producing monoclonal antibodies to pseudorabies virus (prv) were established. the monoclonal antibodies were characterized with respect to their antigenic specifications and biological activities. two monoclonal antibodies immunoprecipitated the 50 kda prv glycoprotein (gp50) and two immunoprecipitated the 82 kda glycoprotein (gp82). the monoclonal antibodies were used to analyze the biological roles of these two glycoproteins. one monoclonal antibody directed against each g ... | 1985 | 3002067 |
| experimental infection of fattening pigs with pseudorabies (aujeszky's disease) virus: efficacy of attenuated live- and inactivated-virus vaccines in pigs with or without passive immunity. | the efficacies of attenuated live- and inactivated-virus vaccines against pseudorabies (pr) in fattening pigs were compared. pigs born from vaccinated or nonvaccinated sows were vaccinated with one or the other vaccine and were challenge exposed at the end of the fattening period. the particular form of pr observed in fattening units in the field could be reproduced. a marked difference was seen between the control lot and the lots of the pigs vaccinated with the attenuated live- and inactivated ... | 1985 | 2992327 |
| protein kinase activities associated with the virions of pseudorabies and herpes simplex virus. | protein kinase has been extracted in soluble form from virions of pseudorabies virus using 10% np40, 0.6 m-nacl. chromatographic analysis of the extract on deae-cellulose and on phosphocellulose showed it to contain more than one kinase. the activity responsible for the phosphorylation of the major phosphoproteins (mol. wts. 120 000, 115 000 and 72 000) of virions was found to be similar in its properties to the host enzyme casein kinase ii. purified casein kinase ii from ascites cells or from p ... | 1985 | 2984312 |
| attenuated properties of thymidine kinase-negative deletion mutant of pseudorabies virus. | a thymidine kinase (tk)-negative (tk-) deletion mutant of the bucharest (buk) strain of pseudorabies virus (prv) was isolated. the mutant, designated as prv (buk d13), did not revert to tk-positive (tk+), even when propagated in medium that selected for tk+ viruses. the mutant also replicated equally well at 39.1 c and 34.5 c, and was easily distinguished from other prv strains by molecular hybridization experiments, restriction nuclease fingerprints, and plaque autoradiography or other assays f ... | 1985 | 2992322 |
| pathologic changes in pigs with prednisolone-induced recrudescence of herpesvirus infection. | in pigs inoculated with aujeszky's disease virus (adv), recrudescence of herpesvirus infection was induced by daily administration of 1,000 mg of prednisolone for 5 days at 2 (group a) or 5 (group b) months after the primary infection. at necropsy in group a pigs, adv was recovered from nasal secretions 3 to 9 days after prednisolone treatment initiation and from the brain cortex 10 days after treatment initiation; adv was not recovered from group b pigs. in pigs of both groups killed 10 days af ... | 1985 | 2992328 |
| phagocytosis and intracellular killing of pasteurella multocida by porcine alveolar macrophages after infection with pseudorabies virus. | the purpose of this study was to determine if pseudorabies virus (prv) interfered with normal alveolar macrophage phagocytic functions. porcine alveolar macrophages (pam) obtained by pulmonary lavage were exposed to prv. at 1 hour postinfection, cells were challenged with pasteurella multocida labeled with 3[h] thymidine. the phagocytosis assay was performed by measuring total radioactivity 1 hour after pm challenge in a soft-beta spectrophotometer. intracellular killing was measured by counting ... | 1986 | 3020772 |
| antiviral effectiveness of butylated hydroxytoluene against pseudorabies (aujeszky's disease) virus in cell culture, mice, and swine. | butylated hydroxytoluene (bht) was evaluated for antiviral effectiveness on pseudorabies virus (prv) in cell culture, mice, and swine. when relatively small amounts of bht were mixed with prv and incubated at 37 c for 30 or 60 minutes before inoculation into cell cultures, the cell cultures did not become infected with virus. the prv was not infectious when the virus was treated with bht and then inoculated intraperitoneally into mice, but was infectious when bht and prv were inoculated simultan ... | 1986 | 3021025 |
| inhibition of african swine fever infection in the presence of immune sera in vivo and in vitro. | in assay cultures, sera from african swine fever convalescent pigs inhibited infection by homologous african swine fever virus. the infection-inhibition capacity did not correspond with the virus-neutralizing capacity. the serum did not prevent infection by heterologous virulent viruses. sera from pigs challenge inoculated with the homologous virulent virus and later with a heterologous virulent virus inhibited the infection by different heterologous virulent viruses. these sera did not interfer ... | 1986 | 3729125 |
| levels of passive antibodies against aujeszky's disease virus in piglets derived from infected sows. | 1986 | 3735896 | |
| [use of immunoenzyme (elisa) diagnosis of aujeszky's disease in pigs during the recovery period]. | samples of blood and blood serums of pigs were examined for the presence of antibodies to the aujeszky's disease virus. the virus-neutralizing (vn) test and the enzymoimmunologic (elisa) method were used for this examination. as indicated by comparison of the average titres of antiviral antibodies determined by both methods, the elisa method is 60 to 600 times more sensitive than the vn test. the high sensitivity of the elisa method enabled to detect antiviral antibodies even in samples consider ... | 1986 | 3022451 |
| herpesvirus (pseudorabies virus) latency in swine: occurrence and physical state of viral dna in neural tissues. | the occurrence of the pseudorabies virus (prv, herpes suis 1) genome in various neural tissues of latently infected pigs was investigated. during the latent phase of infection, between 7 and 52 weeks p.i., the average amount of prv dna ranged between 0.3 and 0.05 genome copies per cell. the results obtained by in situ cytohybridization and reassociation kinetic experiments indicated that each latently infected cell harbored at least 30 viral genome copies. prv dna could be demonstrated in simila ... | 1986 | 3024403 |
| [induction of biological protection in pigs against infection with aujeszky disease virus by vaccination with large doses of live or inactivated vaccines]. | pigs were inoculated against the aujeszky's disease twice in a four-week interval. the dose of the live vaccine was 10(6) tkid50 and the titres of neutralizing antibodies were 1 : 16 to 1 : 128 in blood serum. two weeks later the pigs were exposed to contact infection. primary multiplication of the virus was observed on the mucous membranes of the nose and oropharynx and the virus was detected on the nasal mucous membrane within one to five days, the maximum infection titre values being 10(1.3) ... | 1986 | 3008403 |