Publications

genomic characterization of pseudorabies virus strains isolated in italy.	in this study, we undertook the genomic characterization of 54 pseudorabies virus (prv) strains isolated in italy during 1984-2010. the characterization was based on partial sequencing of the ul44 (gc) and us8 (ge) genes; 44 strains (38 for gene ge and 36 for gc) were isolated on pig farms; 9 originated from dogs and 1 from cattle. these porcine prv strains, which were closely related to those isolated in europe and america in the last 20 years, and the bovine strain bovine/it/2441/1992 belong t ...	2014	23331342
first reports of pseudorabies and winter ticks (dermacentor albipictus) associated with an emerging feral swine (sus scrofa) population in new hampshire.	the expansion of feral swine (sus scrofa) populations into new geographic regions is of concern not only due to increased range but also because they carry diseases and parasites that pose a threat to humans, livestock, and wildlife into new areas. recently, emerging feral swine populations have been reported in the northeastern us and due to their adaptive nature will likely continue to spread. during 2009-2012, 49 feral swine were removed from three counties in new hampshire. of these, serum s ...	2014	24171577
spatio-temporal analysis of the progression of aujeszky's disease virus infection in wild boar of saxony-anhalt, germany.	aujeszky's disease (ad, pseudorabies) is a notifiable disease caused by suid herpesvirus 1 (suhv-1), also named pseudorabies virus (prv). the study aimed at determining the occurrence and spatio-temporal trend of specific antibodies to ad virus (adv) among wild boar of saxony-anhalt, a landlocked federal state situated in the western part of eastern germany. to this end, a total of 7,209 blood samples were collected and tested from 2000 to 2011. an average seroprevalence of 6.8% was found for th ...	2013	24258896
swine infectious agents in tayassu pecari and pecari tajacu tissue samples from brazil.	peccaries and pigs, tayassuidae and suidae respectively, diverged approximately one million years ago from a common ancestor. because these families share some pathogens, peccaries can act as reservoirs of infectious pathogens for domestic and wild swine. we evaluated the presence of swine infectious agents in the spleen and lung tissues of white-lipped peccaries (wlp; tayassu pecari) and collared peccaries (cp; pecari tajacu) in brazil. samples from 10 adult cp and three wlp, which had been hun ...	2014	24484498
serologic, molecular, and pathologic survey of pseudorabies virus infection in hunted wild boars (sus scrofa) in italy.	to investigate pseudorabies-virus (prv) -antibody and viral-dna prevalence, we collected blood, nasal and genital swabs, and tonsillar and lymph-node tissue samples from 139 wild boars (sus scrofa; 39 piglets, 30 juveniles, and 70 adults), during the hunting season of 2010-2011 in tuscany, central italy. we performed immunohistochemistry with anti-prv monoclonal antibodies on selected tissue samples. forty-three of 139 (30.9%) boars were prv-antibody positive and a 1,954-base-pair prv-specific p ...	2014	24779458
molecular characterization of belgian pseudorabies virus isolates from domestic swine and wild boar.	aujeszky's disease is an economically important disease in domestic swine caused by suid herpesvirus 1, also called pseudorabies virus (prv). in several european countries, including belgium, the virus has successfully been eradicated from the domestic swine population. the presence of prv in the wild boar population however poses a risk for possible reintroduction of the virus into the domestic pig population. it is therefore important to assess the genetic relatedness between circulating strai ...	2014	24908275
long-term monitoring of 10 selected pathogens in wild boar (sus scrofa) in sierra nevada national park, southern spain.	wild boar (sus scrofa) populations are increasing in the iberian peninsula, and population management must include disease management and control. in this study, the epidemiology of 10 selected pathogens (aujeszky's disease virus - adv, porcine reproductive and respiratory syndrome virus - prrsv, porcine influenza virus, porcine circovirus, porcine parvovirus, erysipelotrix rhusiopathiae, leptospira pomona, chlamydia/chlamydiaceae sp., salmonella sp. and mycobacterium bovis) in the wild boar pop ...	2014	25261921
severity of bovine tuberculosis is associated with co-infection with common pathogens in wild boar.	co-infections with parasites or viruses drive tuberculosis dynamics in humans, but little is known about their effects in other non-human hosts. this work aims to investigate the relationship between mycobacterium bovis infection and other pathogens in wild boar (sus scrofa), a recognized reservoir of bovine tuberculosis (btb) in mediterranean ecosystems. for this purpose, it has been assessed whether contacts with common concomitant pathogens are associated with the development of severe btb le ...	2014	25350002
characterisation of pseudorabies virus in domestic pigs and wild boars in croatia.	serological data imply that pseudorabies (aujeszky's disease) is present in domestic pigs and wild boars in croatia. therefore, this study included testing of brain tissue samples collected from 200 domestic pigs and 105 wild boars originating from seventeen districts of croatia. the presence of pseudorabies virus (prv) dna was confirmed in samples originating from six domestic pigs (3%) and one wild boar (0.95%). positive samples were sequenced and analysed on the basis of a gc genome fragment. ...	2014	25410393
exposure of extensively farmed wild boars (sus scrofa scrofa) to selected pig pathogens in greece.	increased density and distribution of wild boar populations are likely to promote interactions and transmission of certain pathogens, not only among wild boar but also from wild boar to livestock or humans and vice versa.	2015	25774434
serological prevalence of viral agents that induce reproductive failure in south korean wild boar.	viral agents associated with reproductive failure such as aujeszky's disease virus (adv), encephalomyocarditis virus (emcv), and porcine parvovirus (ppv) have also been identified in european wild boar. to screen for the presence of antibodies against adv, emcv, and ppv from wild boar (sus scrofa) in south korea, 481 serum samples were collected from wild boar hunted between december 2010 and may 2011.	2015	25888836
detection and molecular analysis of pseudorabies virus strains isolated from dogs and a wild boar in italy.	aujeszky's disease (ad) is one of the most economically important diseases of farmed pigs. wild boars can act as reservoirs and might represent a potential threat for domestic animals, including dogs. the aim of this study was to report the results of an ad survey based on the pseudorabies virus (prv) genome detection in samples of dogs clinically suspected of ad and of wild boars collected during four consecutive hunting seasons in the period 2010-2014. genomic characterization was based on the ...	2015	25912160
a serosurvey for selected pathogens in greek european wild boar.	serum samples, collected from 94 european wild boar (sus scrofa) during the hunting seasons 2006 -2010 from different regions of greece, were examined in order to estimate the role of these wildlife species as reservoir of pathogens important for livestock and/or public health.	2015	26392908
shedding patterns of endemic eurasian wild boar (sus scrofa) pathogens.	the eurasianwild boar has experienced aworldwide demographic explosion that increases awareness on shared pathogens. however, shedding routes of relevant wild boar pathogens are unknown. previous observations on sex- and age-related differences in aujeszky's disease virus (adv) exposure led us to hypothesize that shedding patterns of endemicwild boar pathogens may be influenced by individual traits.we investigated shedding routes of adv, porcine parvovirus (ppv), porcine circovirus type 2 (pcv2) ...	2015	26412545
a picture of trends in aujeszky's disease virus exposure in wild boar in the swiss and european contexts.	in parallel to the increase of wild boar abundance in the past decades, an increase of exposure to the aujeszky's disease virus (adv) has been reported in wild boar in several parts of europe. since high animal densities have been proposed to be one of the major factors influencing adv seroprevalence in wild boar populations and wild boar abundance has increased in switzerland, too, a re-evaluation of the adv status was required in wild boar in switzerland. we tested wild boar sera collected fro ...	2015	26545869
age- and strain-dependent differences in the outcome of experimental infections of domestic pigs with wild boar pseudorabies virus isolates.	although pseudorabies virus (prv) has been eradicated in domestic swine in many countries, its presence in wild boars remains a threat for a reintroduction into the currently unprotected swine population. to assess the possible impact of such a reintroduction in a naive herd, an in vivo infection study using two genetically characterized wild boar prv isolates (bel24043 and bel20075) representative for wild boar strains circulating in south-western and central europe and the virulent nia3 refere ...	2016	26589961
pseudorabies virus isolates from domestic pigs and wild boars show no apparent in vitro differences in replication kinetics and sensitivity to interferon-induced antiviral status.	pseudorabies virus is the causative agent of aujeszky's disease. domestic pigs and wild boars are its natural hosts, and strains circulating within both populations differ in their capacity to induce clinical disease. cell biological and molecular explanations for the observed differences in virulence are, however, lacking. different virulence determinants that can be assessed in vitro were determined for five domestic swine strains, four wild boar strains and the nia3 reference strain. replicat ...	2016	26590089
antiviral activities of 2,6-diaminopurine-based acyclic nucleoside phosphonates against herpesviruses: in vitro study results with pseudorabies virus (prv, suhv-1).	pseudorabies virus (prv), a causative agent of aujeszky's disease, is deadly to most mammals with the exception of higher primates and men. this disease causes serious economic loses among farm animals, especially pigs, yet many european countries are today claimed to be aujeszky's disease free because of the discovery of an efficient vaccination for pigs. in reality, the virus is still present in wild boar. current vaccines are neither suitable for dogs nor are there anti-prv drugs approved for ...	2016	26854349
pseudorabies virus and brucella abortus from an expanding wild pig ( sus scrofa ) population in southern oklahoma, usa.	wild pigs ( sus scrofa ) are causing increasing ecologic and economic damage at a global scale. because wild pigs can carry ≥65 diseases that affect livestock, their widespread expansion threatens native wildlife and livestock. we screened wild pigs from south-central oklahoma, us for antibodies against brucella abortus , pseudorabies virus (prv), and porcine reproductive and respiratory syndrome virus (prrs). these pathogens were chosen because they are part of eradication programs in the us an ...	2016	27124329
evidence of aujeszky's disease in wild boar in serbia.	aujeszky's disease is a viral disease of suids caused by suid herpesvirus 1. the disease has worldwide distribution with significant economic impact. in serbia, there is neither an aujeszky's disease eradication nor national vaccination programme of domestic pigs. since clinical symptoms of aujeszky's disease are not specific, it is important to establish a link between clinical signs and presence of adv active infection in wild boars. the aim of this study was to investigate the possibility of ...	2016	27357597
evolutionary diversity of suid herpesvirus 1 based on ul44 partial sequences.	the aim of this study was to use partial ul44 sequences (glycoprotein c) of suid herpesvirus 1 to examine the evolution and dynamics of the virus in different periods and hosts.	2016	27362774
an oral aujeszky's disease vaccine (ys-400) induces neutralizing antibody in pigs.	aujeszky's disease (ad) is an economically important disease affecting both wild and domestic pigs of the species sus scrofa. a previous study yielded serological evidence of ad in korean wild boars, which could spread ad to other animals. a new aujeszky's disease virus (adv) bait vaccine is required to prevent ad outbreaks in swine. in the present study, we investigated the safety and immunogenicity of a ge-deleted marker vaccine, strain ys-400, in young domestic pigs.	2016	27489803
leading edge analysis of transcriptomic changes during pseudorabies virus infection.	eight rna samples taken from the tracheobronchial lymph nodes (tbln) of pigs that were either infected or non-infected with a feral isolate of porcine pseudorabies virus (prv) were used to investigate changes in gene expression related to the pathogen. the rna was processed into fastq files for each library prior to being analyzed using illumina digital gene expression tag profiling sequences (dgetp) which were used as the downstream measure of differential expression. analyzed tags consisted of ...	2016	27766207
pseudorabies virus infection (aujeszky's disease) in an iberian lynx (lynx pardinus) in spain: a case report.	the only natural hosts of pseudorabies virus (prv) are members of the family suidae (sus scrofa scrofa). in species other than suids infection is normally fatal. in these mammals, including carnivores, prv typically causes serious neurologic disease. the endangered iberian lynx (lynx pardinus) is a wild feline endemic to south-western europe (iberian peninsula). the iberian lynx was found to be the world's most endangered felid species in 2002. in wild felines, prv infection has only been previo ...	2017	28056966
harmonizing methods for wildlife abundance estimation and pathogen detection in europe-a questionnaire survey on three selected host-pathogen combinations.	the need for wildlife health surveillance as part of disease control in wildlife, domestic animals and humans on the global level is widely recognized. however, the objectives, methods and intensity of existing wildlife health surveillance programs vary greatly among european countries, resulting in a patchwork of data that are difficult to merge and compare. this survey aimed at evaluating the need and potential for data harmonization in wildlife health in europe. the specific objective was to ...	2017	28202055
global mammal parasite database version 2.0.	illuminating the ecological and evolutionary dynamics of parasites is one of the most pressing issues facing modern science, and is critical for basic science, the global economy, and human health. extremely important to this effort are data on the disease-causing organisms of wild animal hosts (including viruses, bacteria, protozoa, helminths, arthropods, and fungi). here we present an updated version of the global mammal parasite database, a database of the parasites of wild ungulates (artioda ...	2017	28273333
survey for selected pathogens in wild pigs (sus scrofa) from guam, marianna islands, usa.	pigs (sus scrofa) were introduced to guam in the 1600's and are now present in high densities throughout the island. wild pigs are reservoirs for pathogens of concern to domestic animals and humans. exposure to porcine parvovirus, transmissible gastroenteritis, and leptospira interrogans has been documented in domestic swine but data from wild pigs are lacking. the close proximity of humans, domestic animals, and wild pigs, combined with the liberal hunting of wild pigs, results in frequent oppo ...	2017	28622856
host and viral traits predict zoonotic spillover from mammals.	the majority of human emerging infectious diseases are zoonotic, with viruses that originate in wild mammals of particular concern (for example, hiv, ebola and sars). understanding patterns of viral diversity in wildlife and determinants of successful cross-species transmission, or spillover, are therefore key goals for pandemic surveillance programs. however, few analytical tools exist to identify which host species are likely to harbour the next human virus, or which viruses can cross species ...	2017	28636590
reduced virulence of a pseudorabies virus isolate from wild boar origin in domestic pigs correlates with hampered visceral spread and age-dependent reduced neuroinvasive capacity.	morbidity and mortality associated with pseudorabies virus (prv) infection are dependent on the age of the pig and the virulence of the strain. prv strains circulating in wild boar are considered to be low virulent, but no mechanistic explanation for their reduced virulence is available. here infection of 2- and 15-week-old domestic pigs with the prv wild boar strain bel24043 did not induce clinical symptoms in 15-week-old pigs, but resulted in important neurological and respiratory disease in 2 ...	2017	28873002
evidence of pseudorabies virus shedding in feral swine ( sus scrofa) populations of florida, usa.	: feral swine ( sus scrofa) are a pathogen reservoir for pseudorabies virus (prv). the virus can be fatal to wildlife and contributes to economic losses in the swine industry worldwide. national surveillance efforts in the us use serology to detect prv-specific antibodies in feral swine populations, but prv exposure is not a direct indicator of pathogen transmission among conspecifics or to non-suid wildlife species. we measured antibody production and the presence of prv dna in four tissue typ ...	2018	28982018