Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| novel recombinant parapoxvirus vectors induce protective humoral and cellular immunity against lethal herpesvirus challenge infection in mice. | orf virus (orfv; parapoxvirus ovis) was used to develop a novel vector system for the generation of effective and safe live vaccines. based on the attenuated orfv strain d1701-v, recombinants were produced that express the glycoproteins gc (d1701-vrvgc) or gd (d1701-vrvgd) of the alphaherpesvirus of swine, pseudorabies virus (prv). expression of gc and gd was also demonstrated on the surface of recombinant virus-infected murine cells that do not produce infectious orfv. single or combined immuni ... | 2003 | 12915547 |
| exogenous porcine viruses. | porcine organs, cells and tissues provide a viable source of transplants in humans, though there is some concern of public health risk from adaptation of swine infectious agents in humans. limited information is available on the public health risk of many exogenous swine viruses, and reliable and rapid diagnostic tests are available for only a few of these. the ability of several porcine viruses to cause transplacental fetal infection (parvoviruses, circoviruses, and arteriviruses), emergence or ... | 2003 | 12934944 |
| manufacturing process of anti-thrombin iii concentrate: viral safety validation studies and effect of column re-use on viral clearance. | a manufacturing process for the production of anti-thrombin iiii concentrate is described, which is based primarily on heparin sepharose affinity chromatography. the process includes two sequential viral inactivation/removal procedures, applied to the fraction eluted from the column, the first by heating in aqueous solution at 60 degrees c for 10 h and the second by nanofiltration. using viral validation on a scaled-down process both treatments proved to be effective steps; able to inactivate or ... | 2003 | 12935804 |
| neurological disorders associated with aujeszky's disease virus infection in fattening pigs. | 2003 | 12967147 | |
| application of a quantitative algorithm to restriction endonuclease analysis of aujeszky's disease (pseudorabies) virus from a geographically localized outbreak. | a retrospective epidemiologic study was conducted to evaluate the application of an objective quantitative algorithm for estimating genetic similarity from restriction endonuclease analysis data. the analysis was performed to assist the determination of chronologic trends in an aujeszky's disease viral epidemic in a geographic region. dna from each viral isolate obtained during the epidemic was digested with 4 restriction endonucleases and molar ratio labeled to generate separate fragment patter ... | 1999 | 12968755 |
| detection of porcine teschoviruses and enteroviruses by lightcycler real-time pcr. | porcine picornaviruses comprising at least 23 serotypes grouped into six species were described as causative agents of neurological disorders, reproductive failure, and aphthae-like dermal lesions of swine. other viruses such as classical swine fever virus (csfv), african swine fever virus, pseudorabies virus (prv), vesicular stomatitis virus, vesicular exanthema virus, porcine respiratory and reproductive syndrome virus, and porcine parvovirus (ppv) may cause diseases with similar clinical symp ... | 2003 | 14500127 |
| aujeszky's disease in fattening pigs. | 2003 | 14516122 | |
| application of phylogeny reconstruction and character-evolution analysis to inferring patterns of directional microbial transmission. | i used phylogenetic analyses to reconstruct patterns of directional interspecific transmission during a pseudorabies virus outbreak in illinois, usa, in 1989. isolates were recovered from five species: cattle, sheep, goats, pigs, and raccoons (procyon lotor). i generated dna sequences for 16 isolates of pseudorabies virus at the glycoprotein c gene, from which i constructed phylogenetic trees. i then used these trees, in combination with parsimony-based analyses of character evolution, to infer ... | 2003 | 14516717 |
| spatial relative risk mapping of pseudorabies-seropositive pig herds in an animal-dense region. | this paper describes a new approach for spatial relative risk mapping as a tool for geographical risk assessment. the spatial epidemiological analysis is based on geographically referenced data about pseudorabies (aujeszky's disease) virus infections at farm level in a region of high animal density in germany at the beginning of the national eradication project. on the basis of serological findings 186 farms were classified as positive out of a total of 482 investigated farms listed in veterinar ... | 2003 | 14535929 |
| potential sites of virus latency associated with indigenous pseudorabies viruses in feral swine. | free-ranging feral swine (sus scrofa) are known to be present in at least 32 states of the usa and are continuously expanding their range. infection with pseudorabies virus (prv) occurs in feral swine and the primary route of transmission in free-living conditions seems to be venereal. between 1995 and 1999, naturally infected feral swine and experimentally infected hybrid progeny of feral and domestic swine, were kept in isolation and evaluated for occurrence of latent prv indigenous to feral s ... | 2003 | 14567217 |
| pseudorabies virus (prv)-specific antibodies suppress intracellular viral protein levels in prv-infected monocytes. | blood monocytes infected with pseudorabies virus (prv), a swine alphaherpesvirus, are not eliminated efficiently by antibody-dependent immunity and may occasionally transport prv to the pregnant uterus of vaccinated animals. this study examines in vitro the long-term fate of prv-infected monocytes cultivated in the presence of porcine prv-specific antibodies. all monocytes were infected and expressed viral late proteins, and 30 % of prv-infected monocytes cultivated with prv-specific antibodies ... | 2003 | 14573801 |
| the pseudorabies virus us2 protein, a virion tegument component, is prenylated in infected cells. | the us2 gene is conserved among alphaherpesviruses, but its function is not known. we demonstrate here that the pseudorabies virus (prv) us2 protein is synthesized early after infection and localizes to cytoplasmic vesicles and to the plasma membrane, despite the lack of a recognizable signal sequence or membrane-spanning domain. us2 protein is also packaged as part of the tegument of mature virions. the us2 carboxy-terminal four amino acids comprise a caax motif, a well-characterized signal for ... | 2003 | 14581565 |
| are hypothalamic neurons transsynaptically connected to porcine adipose tissue? | specific anatomical sites and pathways responsible for mediating metabolic and neuroendocrine effects of leptin are still poorly understood. therefore, we examined distribution of leptin receptor-containing neurons transsynaptically connected with the porcine fat tissue by means of combined viral transneuronal tracing and immunohistochemical staining method. pseudorabies virus (prv) was injected into the perirenal fat tissue in pigs, and after survival periods of 3, 5, 7, 9, and 11 days, hypotha ... | 2003 | 14592440 |
| neurons of the rat preoptic area and the raphe pallidus nucleus innervating the brown adipose tissue express the prostaglandin e receptor subtype ep3. | the major effector organ for thermogenesis during inflammation or experimental pyrogen-induced fever in rodents is the brown adipose tissue (bat). prostaglandin e2 (pge2) microinjection into the medial preoptic area (poa) of rats leads to hyperthermia through an increase in bat thermogenesis and induces pyrogenic signal transmission towards the raphe pallidus nucleus (rpa), a brainstem nucleus known to contain sympathetic premotor neurons for bat control. the medial poa has a high expression of ... | 2003 | 14622218 |
| expression of pseudorabies virus ge epitopes in pichia pastoris and its utilization in an indirect prv ge-elisa. | pseudorabies virus glycoprotein e (prv ge) has been recognized as a suitable diagnostic antigen for pseudorabies. in order to produce ge antigen in large quantities and at low cost, a gene fragment encoding prv ge epitopes was expressed in pichia pastoris expression system. sds-page and western blotting revealed that the expression product was two recombinant proteins, approximately 38 and 32 kda, in the culture supernatant of p. pastoris integrant 72 h after induction. protein concentration ass ... | 2003 | 14625049 |
| effect of mild stress in mice latently infected pseudorabies virus. | stress is one of the important factors that induces reactivation of pseudorabies virus (prv) in latently infected pigs. we established a murine model of latent prv infection and examined the effects of mild stress treatment in order to demonstrate that this model simulates natural infection in the pig. latently infected mice excreted prv from the nasal cavity under stress treatments consisting of restraint, exposure to cold or transport. similar reactions have been observed upon treatment with a ... | 2003 | 14625402 |
| specificity of three elisa-ge kits for screening pig meat for antibodies to aujeszky's disease. | muscle samples (20 g) from 2025 pig carcases from aujeszky's disease-free holdings were collected at the slaughterhouse. the samples were frozen and thawed to obtain meat juice, which was then analysed by three elisa-ge test kits in parallel, to assess their specificity. after two cycles of freezing and thawing, 2.2 per cent of the samples were dry. three times more of the samples from the sow carcases than from the finisher carcases yielded insufficient juice (< 220 microl). to validate the res ... | 2003 | 14653341 |
| the neutralization of pseudorabies virus by anti-alpha-galactocyl natural antibody in normal serum. | pseudorabies virus (prv), a member of herpesviridae alphaherpes subfamily, can infect human cells in vitro. however, the transmission to old world primates including humans is strongly restricted. in this study, we report the neutralizing activity of normal human serum against prv grown in cpk cells derived from pig. prv grown in all old world primates-derived cells, which was tested in this study, were not neutralized by normal human serum. the virion of prv grown in cpk cells harbored galalpha ... | 2004 | 14687940 |
| resistance to pseudorabies virus infection in transformed cell lines expressing a soluble form of porcine herpesvirus entry mediator c. | porcine herpesvirus entry mediator c (hvec) is an alphaherpesvirus receptor that binds to virion glycoprotein d (gd). porcine hvec mediates entry of pseudorabies virus (prv), herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) and bovine herpesvirus type 1 (bhv-1). in order to assess the antiviral potential of a soluble form of porcine hvec, vero cells were transformed with the chimeric gene expressing a fusion protein (phvecig) consisting of an extracellular domain of porcine hvec and the fc p ... | 2004 | 14718632 |
| suprachiasmatic nucleus input to autonomic circuits identified by retrograde transsynaptic transport of pseudorabies virus from the eye. | intraocular injection of the bartha strain of pseudorabies virus (prv bartha) results in transsynaptic infection of the hypothalamic suprachiasmatic nucleus (scn), a retinorecipient circadian oscillator. prv bartha infection of a limited number of retinorecipient structures, including the scn, was initially interpreted as the differential infection of a subpopulation of rat retinal ganglion cells, followed by replication and anterograde transport via the optic nerve. a recent report that used a ... | 2004 | 14991563 |
| the use of different vaccination schedules for sows to protect piglets against aujeszky's disease. | several aujeszky's disease virus (adv) vaccination protocols of sows were evaluated with regard to the passive protection conferred on piglets in a recently built commercial farm. three different groups of sows were vaccinated using a bartha k-61 strain. one group received an inactivated vaccine during pregnancy and the other two groups received attenuated vaccines, either during pregnancy (day 65) or on the seventh day of lactation. at farrowing, sows vaccinated during lactation had lower seron ... | 2004 | 14995971 |
| transcriptional response of a common permissive cell type to infection by two diverse alphaherpesviruses. | pseudorabies virus (prv) and herpes simplex virus type 1 (hsv-1) are distantly related alphaherpesviruses whose natural hosts are pigs and humans, respectively. adult infections of natural hosts are mild and rarely lethal. however, both viruses are also able to infect other hosts, often with lethal effects. in this report, we use the paradigm of infection of a common permissive cell type and microarray analysis to determine if these two diverse alphaherpesviruses engage similar or different cell ... | 2004 | 15016872 |
| vaccine-induced t cell-mediated immunity plays a critical role in early protection against pseudorabies virus (suid herpes virus type 1) infection in pigs. | the aim of our study was to evaluate the relative importance of antibody and t cell-mediated immunity in protection against pseudorabies virus (suid herpes virus type 1) infection in pigs. we induced different levels of immune responses by using: (1) a modified live vaccine; (2) the same modified live vaccine with an oil-in-water (o/w) adjuvant; (3) an inactivated vaccine; and (4) the same inactivated vaccine with an o/w adjuvant. subsequently, we challenged pigs with virulent pseudorabies virus ... | 2004 | 15113659 |
| copatching and lipid raft association of different viral glycoproteins expressed on the surfaces of pseudorabies virus-infected cells. | pseudorabies virus (prv) is a swine alphaherpesvirus that is closely related to human herpes simplex virus (hsv). both prv and hsv express a variety of viral envelope glycoproteins in the plasma membranes of infected cells. here we show that at least four major prv glycoproteins (gb, gc, gd, and ge) in the plasma membrane of infected swine kidney cells and monocytes seem to be linked, since monospecific antibody-induced patching of any one of these proteins results in copatching of the others. f ... | 2004 | 15113909 |
| construction of recombinant pseudorabies virus expressing ns1 protein of japanese encephalitis (sa14-14-2) virus and its safety and immunogenicity. | the bivalent genetic engineering vaccine of japanese encephalitis (je) and aujeszkj disease (ad) was developed to provide a novel approach to prevent and control these two diseases. ns1 gene of japanese encephalitis virus (jev) sa14-14-2 strain was produced by reverse transcriptase-mediated pcr (rt-pcr) and was cloned into vector pusk to form recombinant plasmid (designed as pusk-ns1). a co-transfection experiment was performed in porcine kidney (pk-15) cells with pusk-ns1 and the genome of the ... | 2004 | 15121294 |
| bf, hp, dqb and drb are associated with haemolytic complement activity, acute phase protein reaction and antibody response in the pig. | in order to examine the loci factor b (bf), c3, corticotropin releasing hormone (crh), dqb, drb, haptoglobin (hp) and transforming growth factor beta 1 (tgfb1) for association with traits of humoral, specific and unspecific defence f2-animals of a porcine resource family were genotyped at single nucleotide polymorphisms within these loci. haemolytic complement activity in the alternative and classical pathway, c3c and haptoglobin serum concentration and antibody titres were determined immediatel ... | 2004 | 15135987 |
| an approach to a fmd vaccine based on genetic engineered attenuated pseudorabies virus: one experiment using vp1 gene alone generates an antibody responds on fmd and pseudorabies in swine. | foot-and-mouth disease (fmd) and pseudorabies (pr) are two important infectious diseases in swine. an attenuated pseudorabies virus (prv) has been successfully used as a gene delivery vector for the development of live-viral vaccines. in this study, a recombinant prv-vp1 virus was constructed by fusioning the vp1 gene of fmd virus in frame to the n-terminal sequence of the gg gene of prv. to test the protective immunity, 15 fmdv sero-negative white swine were divided into three groups and immuni ... | 2004 | 15149769 |
| multiplex pcr for rapid detection of pseudorabies virus, porcine parvovirus and porcine circoviruses. | a multiplex pcr (mpcr) assay was developed and subsequently evaluated for its effectiveness as a means to simultaneously detect multiple viral infections of swine. specific primers for each of four common dna viruses, namely, pseudorabies virus (prv), porcine circovirus type i (pcv1), porcine circovirus type ii (pcv2), and porcine parvovirus (ppv), were used for testing procedure. the assay was shown to be highly sensitive in that as little as 10(-4) ng of each of the respective amplicons (appro ... | 2004 | 15223125 |
| characteristics of oligodeoxyribonucleotides that induce interferon (ifn)-alpha in the pig and the phenotype of the ifn-alpha producing cells. | the immunostimulatory effects of oligodeoxyribonucleotides (odn) containing unmethylated cpg dinucleotides (cpg-odn) in certain base contexts have been extensively studied in man and mice. one major action is their ability to trigger production of massive amounts of interferon-alpha (ifn-alpha) by plasmacytoid dendritic cells (pdc), also referred to as natural ifn-alpha/beta producing cells (nipc). the present study using porcine pbmc activated by cpg-odn or plasmid dna revealed a considerable v ... | 2004 | 15261695 |
| impairment of oxidative burst in porcine neutrophils induced by pseudorabies virus. | industrial swine production is affected by several serious viral diseases, such as pseudorabies, hog cholera, porcine reproductive and respiratory syndrome, which are frequently complicated with the increased incidence of bacterial complications such as actinobacillus pleuropneumoniae (app). this clinical observation is suggestive of a virus-bacteria synergism on the pathogenesis. one hypothesis is that viruses induce polymorphonuclear cell (pmns, primarily neutrophils) dysfunction resulting in ... | 2004 | 15261698 |
| internalization of pseudorabies virus glycoprotein b is mediated by an interaction between the yqrl motif in its cytoplasmic domain and the clathrin-associated ap-2 adaptor complex. | the cytoplasmic domain of pseudorabies virus (prv) glycoprotein b (gb) contains three putative internalization motifs. previously, we demonstrated that the tyrosine-based yqrl motif at positions 902 to 905, but not the ymsi motif at positions 864 to 867 or the ll doublet at positions 887 and 888, is required for correct functioning of gb during antibody-mediated internalization of prv cell surface-bound glycoproteins. in the present study, we demonstrate that the yqrl motif is also crucial to al ... | 2004 | 15280493 |
| detection of economically important viruses in boar semen by quantitative realtime pcr technology. | the objective of this study was to develop quantitative real-time polymerase chain reaction (reti-pcr) tests for the detection of five economically important viruses in swine semen namely, pseudorabies virus (prv), classical swine fever virus (csfv), foot-and-mouth disease virus (fmdv), swine vesicular disease virus (svdv), and porcine reproductive and respiratory syndrome virus (prrsv). each reti-pcr test was validated for specificity, analytical sensitivity (detection limits), and experimental ... | 2004 | 15288957 |
| [inactivation of viruses of different taxonomic groups by cuprous sulphate]. | study results of inactivated effects exerted by cuprous sulphate on viruses of different taxonomy groups are summarized in the paper. cuprous sulphate is a simple and reliable agent in inactivation of viruses of classical porcine fever, aujeszky's disease and bovine infectious rhinotracheitis. its inactivation action is based on the ability to reduce the viral genome to low-molecular fragment. apart from inactivation of the virus material, a decreased level of protective antibody determinants is ... | 2004 | 15293511 |
| persistence of pseudorabies virus in feral swine populations. | serologic surveys for evidence of exposure to pseudorabies virus (prv) in feral swine were conducted from november 2001 to april 2002 at 10 sites in the southeastern united states, where evidence of previous prv exposure had been documented during 1979-89. sera were tested in the field on the day of collection by latex agglutination. maximum sample size per site was to be 30 animals, but sampling was discontinued before reaching this number when positive results were obtained. positive results w ... | 2004 | 15362832 |
| spatial risk assessment of herd sero-status of aujeszky's disease in a county in hungary. | a geographic information system (vetepigis) was used to analyze the adv (aujeszky's disease virus) sero-status in large-scale pig units regarding certain geographical features in a county of southern hungary. the adv sero-statuses were collected from all swine units in csongrád county in 1998-2000. the units' coordinates were combined with a vector graphical digital map of the county, with a resolution of 1:100,000. logistic regression tested the associations between sero-status of large-scale u ... | 2004 | 15454323 |
| encephalomalacic lesions in pigs dually infected with porcine reproductive and respiratory syndrome virus and pseudorabies virus. | four pigs (group 1) were infected with an aerosol containing porcine reproductive and respiratory syndrome virus (prrsv) followed 7 days later by pseudorabies virus (prv). three further pigs (group 2) received prrsv alone, two (group 3) received prv alone, and two (group 4) remained as uninfected controls. despite the admittedly small numbers of animals, the experiment appeared to throw light on aspects of synergy. thus, the group 1 pigs showed severe neurological signs characterized by ataxia a ... | 2004 | 15511536 |
| role of il-6 and il-1beta in reactivation by acetylcholine of latently infecting pseudorabies virus. | we previously reported that the latently infecting pseudorabies virus (prv) could be reactivated by injection of swine or mice with acetylcholine. however, the mechanism of the reactivation was not clear yet. in this study, we analyzed the kinetics of cytokines related to stress to clarify the relationship between virus reactivation by acetylcholine and the immune system. il-6 and il-1beta were detected in mice after stimulation with acetylcholine. this shows that acetylcholine induced physiolog ... | 2004 | 15516795 |
| transgenic mice expressing a soluble form of porcine nectin-1/herpesvirus entry mediator c as a model for pseudorabies-resistant livestock. | an approach to genetically engineered resistance to pseudorabies virus (prv) infection was examined by using a transgene encoding a soluble form of nectin-1, also known as herpesvirus entry mediator c. nectin-1 is an alpha-herpesvirus receptor that binds to virion glycoprotein d. nectin-1 mediates entry of prv, herpes simplex virus types 1 and 2, and bovine herpesvirus type 1. to assess the antiviral potential of an ectopic expression of the nectin-1 ectodomain in vivo, six transgenic mouse line ... | 2004 | 15534229 |
| interferon-gamma response of pbmc indicates productive pseudorabies virus (prv) infection in swine. | in chinese meishan/german landrace cross-bred swine f2 generation interferon gamma (ifn-gamma) production by peripheral blood mononuclear cells (pbmc) was determined directly ex vivo at different time points after survival of a virulent pseudorabies virus (prv) infection. this reactivity was compared with the reactivity of naïve pbmc. significant ifn-gamma production was determined in elisa and elispot only after in vitro pbmc re-stimulation with prv and not with the closely related bovine herpe ... | 2004 | 15541792 |
| the pseudorabies virus us3 protein kinase possesses anti-apoptotic activity that protects cells from apoptosis during infection and after treatment with sorbitol or staurosporine. | most large dna viruses, like herpesviruses, encode anti-apoptotic proteins to interfere with the apoptotic cellular response to infection. previous studies have shown that the us3 protein kinase of herpes simplex virus, in contrast to us3 of bovine herpes virus 1, is very potent in protecting cells from apoptosis induced by the virus itself or by a broad range of exogenous apoptotic stimuli. here, we demonstrate that us3 of the swine alphaherpesvirus pseudorabies virus (prv) suppresses prv-induc ... | 2005 | 15582661 |
| enzyme linked immunoassay and fluorescent antibody techniques in the diagnosis of viral diseases using staphylococcal protein-a instead of anti-gamma-globulins. | staphylococcal protein-a (spa) is known to interact with the crystallizable fragment (fc) of igg molecules from several species. in the present study, spa coupled to either fluorescein isothiocyanate (fitc) or peroxidase was used in place of antisera to igg for the fluorescent antibody (fa) techniques and the enzyme linked immunoassay (elisa). the spa conjugates produced low background staining when applied in these techniques, and provide a rapid, highly specific and sensitive means for the ide ... | 1980 | 15612263 |
| viruses in boar semen: detection and clinical as well as epidemiological consequences regarding disease transmission by artificial insemination. | many viruses have been reported to be present in boar semen, particularly during the viremic phase of the diseases. some of them, such foot-and-mouth disease virus, porcine reproductive and respiratory syndrome virus, swine vesicular disease virus, porcine parvovirus, picornaviruses, adenoviruses, enteroviruses, japanese encephalitis virus, pseudorabies virus, african swine fever virus and reoviruses are of particular importance and accurate monitoring prior to and during the presence of boars i ... | 2005 | 15626416 |
| a natural outbreak of aujeszky's disease in farm animals. | an outbreak of aujeszky's disease (ad) occurred in a herd of domestic animals that led to the death of seven cattle, three goats, three sheep, two cats and one dog, all of them with cns signs. the animals were not in direct contact with swine. the adv was detected in the tissue of affected animals by celi culture methods and pcr. genome strains of adv were characterized by restriction endonuclease analysis using bamh i. the results indicated that the strains of virus were identical and belonged ... | 2004 | 15633785 |
| detection of porcine circovirus type 2, porcine parvovirus and porcine pseudorabies virus from pigs with postweaning multisystemic wasting syndrome by multiplex pcr. | multiplex pcr was established to detect porcine circovirus type 2 (pcv-2), porcine parvovirus (ppv) and porcine pseudorabies virus (prv) and applied to samples from 137 piglets exhibiting clinical signs of postweaning multisystemic wasting syndrome (pmws). pcv-2 dna was detected from all samples. moreover, 43 samples were positive for ppv but negative for prv; 11 samples were positive for prv but negative for ppv; and 35 samples were positive both for ppv and prv. these results suggests that pcv ... | 2005 | 15736858 |
| aujeszky's disease vaccination and differentiation of vaccinated from infected pigs. | in this paper, the use of a glycoprotein e (ge)-deleted vaccine to eradicate aujeszky's disease virus (adv) or pseudorabies virus in swine is described. in regions where there is a dense population of swine, adv is highly prevalent and intensive vaccination with such a marker vaccine has resulted, in several countries, in a decrease of the field virus prevalence to a sufficiently low level so that culling becomes economically feasible. the evolution in the reduction of circulating field virus ca ... | 2004 | 15742635 |
| use of a recombinant pseudorabies virus to analyze motor cortical reorganization after unilateral facial denervation. | a unilateral facial nerve injury (n7x) was found to influence the transcallosal spread of the attenuated strain of pseudorabies virus (prv bartha) from the affected (left) primary motor cortex (mi) to the contralateral mi of rats. we used ba-duplac, a recombinant prv strain, for the tracing experiments since this virus was demonstrated to exhibit much more restricted transportation kinetics than that of prv bartha, and is therefore more suitable for studies of neuronal plasticity. ba-duplac inje ... | 2005 | 15749981 |
| evaluation of specific humoral immune response in pigs vaccinated intradermally with deleted aujeszky's disease vaccine and challenged with virulent strain of herpesvirus suis type 1. | a comparison of intradermal (id) versus intramuscular (im) routes of pig vaccination with deleted aujeszky's disease (ad) vaccine on the formation of specific postvaccinal and postchallenge humoral immune response was performed. the studies were carried out on 21 eight week-old piglets, divided into three groups--two experimental and one control of 7 piglets each. animals of first two groups were vaccinated twice in 12 and 16 week of age with deleted, live attenuated ad vaccine porcilis begonia ... | 2005 | 15794468 |
| pseudorabies virus infections in wild boar: data visualisation as an aid to understanding disease dynamics. | spatio-temporal survey data often form the main decision support in disease management. consequently, disease managers rely on adequate description of the data supplied from epidemiological analysis. practically speaking, such a description comprises of a visualisation, a deduced hypothesis and a statistical evaluation of the hypothesis. prior to the application of traditional methods of data visualisation and hence data description, an aggregation of the data is required. this paper illustrates ... | 2005 | 15795014 |
| targeting of herpesvirus capsid transport in axons is coupled to association with specific sets of tegument proteins. | the capsids of neurotropic herpesviruses have the remarkable ability to move in specific directions within axons. by modulating bidirectional capsid transport to favor either retrograde (minus-end) or anterograde (plus-end) motion, these viruses travel to sensory ganglia or peripheral tissue at specific stages of infection. by using correlative motion analysis to simultaneously monitor the trafficking of distinct viral proteins in living neurons, we demonstrate that viral "tegument" proteins are ... | 2005 | 15795370 |
| pseudorabies virus propagated in rabbit kidney-derived rk13 cells is neutralized by natural igm antibodies in normal swine serum which specifically lyse host cells. | pseudorabies virus (prv) propagated in rabbit kidney-derived rk-13 cells (prv-rk) was neutralized by serum obtained from specific pathogen-free pigs through the activation of complement. the virus-neutralizing activity of swine serum was lost after treatment with ethylene glycol-bis-aminoethylether-n,n,n',n'-tetraacetic acid (egta) or ethylenediaminetetraacetic acid (edta). anti-c1q and anti-igm antibodies also inhibited virus-neutralizing activity. though igg-depleted swine serum neutralized pr ... | 2005 | 15805723 |
| serosurvey of aujeszky's disease virus infection in european wild boar in spain. | serum samples from 693 hunted wild boar (sus scrofa) were analysed by means of a blocking elisa technique, and the mean (se) prevalence of antibodies to aujeszky's disease virus was 44 (4) per cent. all the seropositive wild boar were from south central spain, except for one from central spain, close to the main positive area. in this area, where large game species are increasingly managed for hunting, the seroprevalence was affected by the type of management. more intensively managed population ... | 2005 | 15816194 |
| higher resistance of porcine trigeminal ganglion neurons towards pseudorabies virus-induced cell death compared with other porcine cell types in vitro. | trigeminal ganglion (tg) neurons are important target cells for many alphaherpesviruses, constituting major sites for latency/reactivation events. here, the in vitro kinetics of productive infection of the swine alphaherpesvirus pseudorabies virus (prv) and resulting cell death in primary porcine tg neurons were determined, and these were compared with similar kinetics in many other porcine cell types. confocal microscopy showed that all tg neurons expressed late genes such as viral glycoprotein ... | 2005 | 15831935 |
| effective protection of pigs against lethal pseudorabies virus infection after a single injection of low-dose sindbis-derived plasmids encoding prv gb, gc and gd glycoproteins. | this study compared protection of pigs against lethal pseudorabies virus (prv) infection induced by a single injection of various quantities of sindbis virus-derived plasmids encoding prv glycoproteins gb, gc and gd. pigs were injected with 340, 68 or 13 microg of each plasmid. few immune differences were observed after dna injection and more importantly the pigs of the three groups were equally protected against virulent prv infection. single-shot injection of 13 microg of each prv glycoprotein ... | 2005 | 15837372 |
| identification of viral pathogens in aborted fetuses and stillborn piglets from cases of swine reproductive failure in spain. | the objective of the present study was to determine the presence of recognised abortifacient viruses such as porcine reproductive and respiratory virus (prrsv), aujeszky's disease virus (adv), porcine parvovirus (ppv) and porcine circovirus type 2 (pcv2), in tissues from aborted fetuses and stillborn neonates in cases of late reproductive failure in swine. a total of 293 specimens (fetuses aborted in the last third of gestation and stillborn piglets) from 100 different cases of late-term abortio ... | 2005 | 15848788 |
| pseudorabies virus glycoprotein gd contains a functional endocytosis motif that acts in concert with an endocytosis motif in gb to drive internalization of antibody-antigen complexes from the surface of infected monocytes. | viral glycoproteins gb and gd of the swine alphaherpesvirus pseudorabies virus (prv), which is closely related to human herpes simplex virus and varicella-zoster virus, are able to drive internalization of antibody-antigen complexes that may form at the cell surface of infected monocytes, thereby protecting these cells from efficient antibody-mediated lysis. we found earlier that gb relies on an endocytosis motif in its cytoplasmic domain for its function during this internalization process. her ... | 2005 | 15890963 |
| new developments in tracing neural circuits with herpesviruses. | certain neurotropic viruses can invade the nervous system of their hosts and spread in chains of synaptically connected neurons. consequently, it is possible to identify entire hierarchically connected circuits within an animal. in this review, we discuss the use of neurotropic herpesviruses as neuronal tract tracers. although a variety of tract tracing viruses are available, each with its own unique infection characteristics, we focus on the widespread use of attenuated strains of pseudorabies ... | 2005 | 15893400 |
| immunization with pseudorabies virus harboring fc domain of igg makes a contribution to protection of mice from lethal challenge. | to enhance the efficacy of an inactivated vaccine against pseudorabies virus (prv), we evaluated the adjuvant properties of fc domain of igg. a cell line expressing mouse igg fc chimera on its surface was established. we found that when prv was propagated in the cells expressing the fc chimera, prv virion incorporated the fc. immunization of balb/c mice with inactivated prv harboring fc, which had been propagated in the cells expressing fc on its surface, induced higher antibody production again ... | 2005 | 15893614 |
| cpg motif in atcgat hexamer improves dna-vaccine efficiency against lethal pseudorabies virus infection in pigs. | this study evaluated the adjuvant activity of a porcine-specific type a cpg-containing oligodeoxynucleotide (cpg-odn) in a dna-vaccine against pseudorabies virus (prv) infection. this vaccine, composed of three independent plasmids encoding prv gb, gc and gd glycoproteins, was injected alone or was co-injected with cpg-odn or control gpc-odn. cpg-odn improved the prv-specific humoral immune response and provided better clinical protection against lethal prv-infection. the prv-specific cell-media ... | 2005 | 15921828 |
| the co-administrating of recombinant porcine il-2 could enhance protective immune responses to prv inactivated vaccine in pigs. | three candidate cytokines: recombinant porcine interleukin-2 (rpil-2), rpil-6 and the fusion protein rpil6-il2 were used as adjuvants in this study to investigate the enhanced immune responses to prv inactivated vaccine (iav) in pigs. in this natural host trial, we demonstrated that rpil-2 showed potential adjuvant effects on prv iav, which was characterized not only in antigen-specific immune responses, but also in protection against prv infection. the use of rpil-2 resulted in significantly hi ... | 2005 | 15946776 |
| cytoskeletal rearrangements and cell extensions induced by the us3 kinase of an alphaherpesvirus are associated with enhanced spread. | the us3 protein is a viral kinase that is conserved among the alphaherpesvirinae. here, we show that us3 of the swine alphaherpesvirus pseudorabies virus causes dramatic alterations in the cytoskeleton, resulting in the formation of long actin- and microtubule-containing cell projections in infected and transfected cells. analysis with a gfp-labeled virus showed that multiple virus particles move inside the projections toward the tip. gfp-labeled virus could also be found in the cytoplasm of nei ... | 2005 | 15951429 |
| protective immunity induced by a recombinant pseudorabies virus expressing the gp5 of porcine reproductive and respiratory syndrome virus in piglets. | pseudorabies virus (prv) has been developed as a vaccine vector for expressing foreign immunogens. porcine reproductive and respiratory syndrome (prrs), caused by porcine reproductive and respiratory syndrome virus (prrsv), continues to be a major problem to the pork industry worldwide. many vaccine strategies have been developed to control the disease but most of them turn out to be unsuccessful. the objective of this research was to explore the feasibility of prv-based vector vaccine in protec ... | 2005 | 15963827 |
| [expression of truncated ge gene of pseudorabies virus (prv) and primary application in differential diagnosis of prv vaccination and infection]. | with the application of ge gene deleted pseudarabies virus (prv) vaccine worldwide, a corresponding differential diagnosis based on ge glycoprotein is needed in the project of prv eradication. in this study, prv ge gene without signal and transmembrane region was amplified by pcr and cloned into pgex-6p-1, generated pgex-ge. after transformation of bl21 with pgex-ge, an expressed fusion protein(about 63kd) was identified by sds-page. the recombinant proteins are produced as inclusion bodies. by ... | 2004 | 15968982 |
| [construction and characterization of a pseudorabies virus tk-/gg- mutant]. | to construct a tk-/gg- mutant of pseudorabies virus, the gg-detected transfer vector puskkbb and genomic dna of pseudorabies virus tk-/gg-/lacz+ were co-transfected into ibrs-2 cells. transfection progeny were plated onto pk-15 cells and incubated for 2 days under methylcellulose. then the overlay was removed and replaced by 1% low melting point agarose in dmem supplemented with 150 microg/ml x-gal. after 2 days, white plaques were screened for and purified 4 times. by pcr amplification of gg-de ... | 2004 | 15968983 |
| autonomic brainstem nuclei are linked to the hippocampus. | vagal nerve stimulation has been reported to enhance memory in both rats and humans, and to be an effective treatment for epilepsy in some patients, but the underlying neuroanatomical substrate(s) responsible for these effects remains unknown. since there is no direct anatomical projection from the nucleus tractus solitarius, the main vagal relay site of the brain, to the hippocampus, we tested whether a multisynaptic pathway exists. pseudorabies virus, a pig herpesvirus that can be used as a re ... | 2005 | 15975727 |
| dimerization of glycoprotein e(rns) of classical swine fever virus is not essential for viral replication and infection. | the pestivirus glycoprotein e(rns), a ribonuclease, is expressed on the surface of virions and in infected cells as a disulfide-linked homodimer. e(rns) is involved in the infection process and its rnase activity is probably involved in viral replication and pathogenesis. the most c-terminal cysteine residue forms an intermolecular disulfide bond with another e(rns) monomer, resulting in an e(rns) dimer. to study the function of dimerisation of e(rns) for viral replication, the cysteine residue ... | 2005 | 15986175 |
| immunogenicity of a recombinant pseudorabies virus expressing orf1-orf2 fusion protein of porcine circovirus type 2. | porcine circovirus type 2 (pcv2) is associated with post-weaning multisystemic wasting syndrome (pmws). pseudorabies (pr) is also an important infectious disease in swine and sometimes co-infect with pcv2. an attenuated pseudorabies virus (prv) has been successfully used as a vector for live viral vaccines. in this study, a recombinant prv expressing orf1-orf2 fusion protein of pcv2 was constructed and its immunogenicity was tested in mice and pigs. the orf1 and partial orf2 gene of pcv2 yu-a st ... | 2005 | 16006077 |
| efficacy of spray-drying to reduce infectivity of pseudorabies and porcine reproductive and respiratory syndrome (prrs) viruses and seroconversion in pigs fed diets containing spray-dried animal plasma. | three experiments were conducted to evaluate viral inactivation by the spray-drying process used in the production of spray-dried animal plasma (sdap). in exp. 1, bovine plasma was inoculated with pseudorabies virus (prv) grown in pk 15 cells. three 4-l batches were spray-dried in the same manner and conditions of industrial sdap production but with laboratory spray-drying equipment. presence of infectivity was determined before and after spray-drying by microtiter assay in pk 15 cell cultures. ... | 2005 | 16024714 |
| pneumonia of pigs: a review. | pneumonia of pigs is one of the more important disease factors limiting pig production. of the varieties of pneumonia affecting this species enzootic pneumonia caused by mycoplasma spp. is the most common and most important. the major effects of this disease are lowered food conversion ratio and poor weight gain. deaths are usually the result of secondary infection by necrotising, pus-forming bacteria. eradication of the disease is expensive and requires depopulation and restocking. control and ... | 1981 | 16030803 |
| mechanisms of infection in the respiratory tract. | related to its potential vulnerability the respiratory tract has a very complex and effective defence apparatus. the interaction between these defence mechanisms and certain characteristics of aetiological agents results in a pattern in which initial infections by these agents tend to occur at specific sites in the tract. infections in which the primary portal of entry is in the upper respiratory tract include bordetella bronchiseptica and haemophilus spp in pigs; pasteurella spp in cattle, shee ... | 1981 | 16030806 |
| a comparison of the efficacy of two commercial aujeszky's disease vaccines with glycoprotein-i deletion in pigs. | two commercial aujeszky's disease vaccines, a modified killed vaccine and a sub-unit vaccine, both carrying a deletion of glycoprotein-i, were evaluated in pigs. each vaccine was administered to two groups of four pigs, twice at 4-week intervals, with two pigs held as unvaccinated controls. all pigs were challenged with a new zealand field isolate of aujeszky's disease virus 3 weeks after the second vaccination. the results indicate that the sub-unit vaccine was able to protect pigs against clin ... | 1993 | 16031687 |
| seroprevalence of six reproductive pathogens in european wild boar (sus scrofa) from spain: the effect on wild boar female reproductive performance. | we studied the seroprevalence of six reproductive pathogens in spanish hunter-harvested wild boar females. the sample was representative of the hunting harvest in the studied hunting estates. mean antibody prevalences were: 60.6+/-0.06% for aujeszky's disease virus (adv), 56.6+/-0.09% for porcine parvovirus (ppv), 51.8+/-0.06% for porcine circovirus type 2 (pcv2), 29.7+/-0.09% for brucella spp. and 36.3+/-0.1% for toxoplasma gondii. we did not detect antibodies against porcine reproductive and r ... | 2006 | 16076482 |
| [recombination and expression of orf1 and orf2 gene of porcine circovirus type 2 and gene of pseudorabies virus]. | orf1 and orf2 gene of porcine circovirus type 2 were cloned by pcr with the specific primers designed according to genome of pcv2 (ay035820). following extraction and digestion, pcr products were subsequently inserted into universal transfer vector plecmv (deleted partial ge and gi of pseudorabies virus) to generate recombinant transfer plasmid pieorf1-orf2. the genomic dna of prv tk-/ge- /lacz+ strain and pieorf1-orf2 were co-transfected into ibrs-2 cells with lipofectin, and recombinant virus ... | 2005 | 16108358 |
| molecular biology of pseudorabies virus: impact on neurovirology and veterinary medicine. | pseudorabies virus (prv) is a herpesvirus of swine, a member of the alphaherpesvirinae subfamily, and the etiological agent of aujeszky's disease. this review describes the contributions of prv research to herpesvirus biology, neurobiology, and viral pathogenesis by focusing on (i) the molecular biology of prv, (ii) model systems to study prv pathogenesis and neurovirulence, (iii) prv transsynaptic tracing of neuronal circuits, and (iv) veterinary aspects of pseudorabies disease. the structure o ... | 2005 | 16148307 |
| effect of transgenic expression of porcine interleukin-6 gene and cpg sequences on immune responses of newborn piglets inoculated with pseudorabies attenuated vaccine. | porcine interleukin-6 gene and cpg sequences were used as immunoadjuvants to enhance the immune responses of newborn piglets to pseudorabies attenuated vaccine (pav). the titer of specific antibodies to pav, the proliferation of lymphocytes and induced il-2 activities were all examined to identify the immune response of the piglets. the results showed that the immune responses with cpg odn and porcine interleukin-6 gene were significantly stronger than routine immunities. the data suggests that ... | 2006 | 16165173 |
| co-administration of porcine-specific cpg oligodeoxynucleotide enhances the immune responses to pseudorabies attenuated virus vaccine in newborn piglets in vivo. | oligonucleotides containing cpg motifs (cpg odn) are strong adjuvants for humoral and cellular immune responses in mice, but data on immune responses in piglets are scarce. in this report, porcine-specific cpg odn were used as immunoadjuvants to enhance the immune responses of the newborn piglets to pseudorabies attenuated virus (prv) vaccine. the titres of specific antibodies and serum igg1/igg2 ratio to prv vaccine, the proliferation of peripheral blood mononuclear cells (pbmcs), il-4 and inte ... | 2006 | 16169588 |
| development of a latex agglutination test using the major epitope domain of glycoprotein e of pseudorabies virus expressed in e. coli to differentiate between immune responses in pigs naturally infected or vaccinated with pseudorabies virus. | a 0.8 kb dna fragment encoding the major epitope domain of glycoprotein e (ge) of pseudorabies virus (prv) was inserted downstream of the t7 promoter of an expression vector, pet-28b, to yield the recombinant plasmid petge804. after induction by isopropy1-beta-d-thiogalactopyranoside (iptg), a high level expression of fusion protein was obtained. sds-page and western immunoblotting analysis showed that the fusion protein was 38 kda and could bind with antisera against prv. the protein existed ma ... | 2005 | 16215839 |
| regulatory considerations for marker vaccines and diagnostic tests in the u.s. | marker vaccines and diagnostic tests can prove to be invaluable in disease eradication and control programs, as was found in the pseudorabies (aujeszky's disease) virus eradication program in the u.s. during that campaign, numerous gene-deleted vaccines and companion diagnostic test kits were used to differentiate infected animals from vaccinated animals, in a strategy that ultimately led to eradication of the disease in commercial swine herds. the united states department of agriculture played ... | 2005 | 16257538 |
| molecular survey of latent pseudorabies virus infection in nervous tissues of slaughtered pigs by nested and real-time pcr. | in this study, the prevalence and quantity of a latent pseudorabies virus (prv) infection in the nervous tissues of randomly selected pigs was determined via nested and real-time pcr. the nervous tissues, including the trigeminal ganglion (tg), olfactory bulb (ob), and brain stem (bs), were collected from the heads of 40 randomly selected pigs. the majority of the nervous tissues from the selected pigs evidenced a positively amplified band on nested pcr. in particular, nested pcr targeted to the ... | 2005 | 16273035 |
| effect of herpesvirus infection on pancreatic duct cell secretion. | to examine the effect of acute infection caused by herpesvirus (pseudorabies virus, prv) on pancreatic ductal secretion. | 2005 | 16273613 |
| determination of the effectiveness of pseudorabies marker vaccines in experiments and field trials. | the aim of vaccination in an eradication campaign is not only to induce clinical protection, but primarily to stop transmission of infections within and between herds by inducing herd immunity. consequently, vaccines should be evaluated for their capacity to reduce virus transmission in the population. glycoprotein e (ge) negative marker vaccines against pseudorabies virus (prv) infections in pigs have been evaluated this way in experiments and field studies. prv infection in groups of (vaccinat ... | 2005 | 16275010 |
| a dna vaccine coding for gb and gd of pseudorabies virus (suid herpes type 1) primes the immune system in the presence of maternal immunity more efficiently than conventional vaccines. | dna vaccines are capable of priming the immune system of neonates in the presence of maternal antibodies. however, it is still not clear whether the extent of priming and protection against challenge infections induced by a dna vaccine in maternally immune newborns is better than that induced by conventional vaccines. to study this, we used the pseudorabies virus (prv) infection model in the natural host, the pig. we compared the efficacy of a dna vaccine with the efficacy of a conventional modi ... | 2006 | 16289276 |
| cell type-specific resistance of trigeminal ganglion neurons towards apoptotic stimuli. | trigeminal ganglion (tg) neurons are important target cells for many alphaherpesviruses and constitute a major site of virus latency and reactivation. earlier we showed that porcine tg neurons are remarkably more resistant towards (apoptotic) cell death resulting from infection by the swine alphaherpesvirus pseudorabies virus (prv) compared to a broad range of other primary porcine cell types and that this resistance does not depend on the strongly anti-apoptotic us3 viral protein kinase (geenen ... | 2006 | 16326038 |
| temporary inhibition of neuronal apoptosis in aujeszky's disease virus-infected swine. | previous studies have shown that during acute infection of the porcine trigeminal ganglia (tg), aujeszky's disease virus (adv)-infected neurons are protected from apoptosis induced by the virus itself and by cells of the immune system. however, tg neurons productively infected by adv finally die and are phagocytosed by adjacent cells, a fact that leads us to speculate that the inhibition of neuronal apoptosis by adv may be temporary rather than absolute. to address this issue we used tg and brai ... | 2006 | 16337347 |
| a homologous in vitro model to study interactions between alphaherpesviruses and trigeminal ganglion neurons. | a key aspect in the life cycle of alphaherpesviruses is their neurotropic behaviour. sensory neurons of the trigeminal ganglion (tg) are important target cells for many alphaherpesviruses (including herpes simplex virus 1, pseudorabies virus (prv), bovine herpesvirus 1) and constitute major sites for latent infections. the aim of this study was to develop an in vitro model that simulates the in vivo infection pattern of tg neurons by alphaherpesviruses. to this end, we developed a homologous in ... | 2006 | 16359830 |
| long-term studies on maternal immunity for aujeszky's disease and classical swine fever in wild boar piglets. | the aim of the studies was to fathom the duration and the role of maternal immunity for aujeszky's disease (ad) and classical swine fever (csf) in wild boar offspring. in one experiment, two wild boar sows were infected with a low pathogenic pseudorabies virus (prv) in 1999. a total of 51 offspring was born between 1999 and 2002 and was monitored for prv maternal antibodies. in a second experiment, the maternal immunity for csf was analysed. therefore, a sow was orally vaccinated against csf usi ... | 2005 | 16364018 |
| the recombinant nonstructural polyprotein ns1 of porcine parvovirus (ppv) as diagnostic antigen in elisa to differentiate infected from vaccinated pigs. | to differentiate pigs infected with porcine parvovirus (ppv) from those vaccinated with inactivated whole-virus vaccine, an enzyme-linked immunosorbent assay (elisa) based on detection of a nonstructural polyprotein 1 (ns1) was developed. a threshold of 0.23 optical density units was established and the assay had high specificity (100), sensitivity (88), accuracy (90) and positive predictive value (100) using haemagglutination inhibition as the standard method. a reproducibility test revealed th ... | 2006 | 16400603 |
| composition of pseudorabies virus particles lacking tegument protein us3, ul47, or ul49 or envelope glycoprotein e. | proteins located in the tegument layer of herpesvirus particles play important roles in the replicative cycle at both early and late times after infection. as major constituents of the virion, they execute important functions in particular during formation of progeny virions. these functions have mostly been elucidated by construction and analysis of mutant viruses deleted in single or multiple tegument protein-encoding genes (reviewed in the work of t. c. mettenleiter, virus res. 106:167-180, 2 ... | 2006 | 16415010 |
| brain lesions in pigs dually infected with porcine reproductive and respiratory syndrome virus and pseudorabies virus. | four pigs were inoculated with an aerosol containing porcine reproductive and respiratory syndrome virus (prrsv) followed 14 days later by inoculation with pseudorabies virus (prv). the four dually infected pigs showed severe clinical signs, and one died on day 6 after infection with prv. as demonstrated previously, the clinical disease was much more severe than that produced by either virus alone. all four dually infected pigs developed severe non-suppurative encephalitis, two had tonsillitis, ... | 2006 | 16423576 |
| comparison of protection levels against pseudorabies virus infection of transgenic mice expressing a soluble form of porcine nectin-1/hvec and vaccinated mice. | we recently generated transgenic mice expressing a soluble form of porcine nectin-1 (phvecig) showing remarkable resistance to pseudorabies virus (prv) infection. nectin-1, also known as herpesvirus entry mediator c (hvec), is an alphaherpesvirus receptor that binds to virion glycoprotein d (gd). in order to evaluate the level of resistance to prv infection induced by the expression of phvecig in the transgenic mice, the protective effects of vaccinated and transgenic mice were directly compared ... | 2006 | 16427216 |
| presence of antibodies against aujeszky's disease virus in wild boar (sus scrofa) in slovenia. | serum samples from 427 hunter-killed wild boar (sus scrofa) from slovenia were tested for antibodies to aujeszky's disease virus (adv). samples were collected throughout slovenia and corresponded to 6.2% of the total harvest. antibodies against adv were detected in 111 sera (26%) using a commercial enzyme-linked immunosorbent assay (elisa). antibody prevalence increased significantly with age. this report describes the first evidence of adv infection in wild boar populations in slovenia. | 2005 | 16456172 |
| a serological survey of selected pathogens in wild boar in slovenia. | serum samples collected from 178 shot wild boars (sus scrofa) were tested for the presence of antibodies against classical swine fever virus, aujeszky's disease virus (adv), porcine reproductive and respiratory syndrome virus, porcine respiratory coronavirus (prcv), transmissible gastroenteritis virus, swine influenza virus, porcine parvovirus (ppv), swine vesicular disease virus, actinobacillus pleuropneumoniae (app), mycoplasma hyopneumoniae, salmonella spp., brucella spp. and haemophilus para ... | 2006 | 16460352 |
| [construction and immunogenicity of recombinant pseudorabies virus expressing the modified gp5m protein of porcine reproduction and respiratory syndrome virus]. | pseudorabies virus (prv), an alpha-herpesvirus, has been used as a vector for live-viral animal vaccines. the recombinant prv tk- / ge- / gp5+, which expressing gp5 of prrsv, is developed based on the prv genetic-depleting vaccine-virus strain, tk- / ge- /lacz+. however, this strain stimulated poorly the vaccinated animals to produce neutralizing antibodies against prrsv. in order to develop a booster specific immunized response of the prv recombinant, the orf5 gene of prrsv tk- / ge- / lacz+ wa ... | 2005 | 16468337 |
| real-time pcr protocol for the detection of porcine parvovirus in field samples. | this report describes a real-time polymerase chain reaction assay with sybr green for detection of a broad range of porcine parvoviruses (ppv) and accurate virus quantification in porcine tissues. the assay targets the vp2 gene of ppv and the porcine genomic c-myc gene for normalization. the detection limit of the sybr green reaction was shown to be equivalent to 6 x 10(0) to 6 x 10(1) ppv copies/reaction and the overall detection limit equivalent to 0.1 tcid(50)/100 microl. the assay was linear ... | 2006 | 16472875 |
| development of solid - based paper strips for rapid diagnosis of pseudorabies infection. | pseudorabies (aujeszky's disease) is an economically significant disease of swine known to cause central nervous disorders, respiratory disease, reproductive failure and mortality in infected pigs. in attempts to eradicate the disease from becoming endemic, early detection is important to prevent further economic losses and to allow for detection and removal of infected pigs in domestic herds. thus, a rapid and sensitive technique is necessary for the detection of the virus. for rapid and simple ... | 2004 | 16493404 |
| investigation of pseudorabies virus latency in nervous tissues of seropositive pigs exposed to field strain. | the prevalence and quantity of latent pseudorabies virus (prv) in nervous tissues of pigs exposed to field strain in korea was investigated by nested and real-time pcr. nervous tissues including trigeminal ganglion (tg), olfactory bulb (ob), and brain stem (bs) were collected from 94 seropositive pigs. prv latent infection in nervous tissues was initially investigated by nested pcr targeting three glycoprotein genes (gb, ge, and gg). based on the obtained result, latent infection was detected in ... | 2006 | 16520536 |
| evaluation of efficacy and safety of aujeszky's disease vaccines. | since aujeszky's disease have become an economic problem in pig farms in late 1960's and early 1970's many different vaccines, either inactivated or live - attenuated were developed. soon it became evident that they differ in their efficacy. in this article a panel of tests used for evaluation of safety and efficacy of inactivated as well as live aujeszky's disease vaccines is described. | 2006 | 16573279 |
| neuronal pathways of viral invasion in mice after intranasal inoculation of pseudorabies virus prv-9112c2 expressing bovine herpesvirus 1 glycoprotein b. | in contrast to wild-type pseudorabies virus (prv), which infects the central nervous system mainly via fibres of the trigeminal and autonomous nerves, the prv mutant prv-9112c2, deleted in glycoprotein b but expressing its bovine herpesvirus 1 (bhv-1) homologue, was shown to infect the swine central nervous system (cns) via the olfactory route. in this study application of prv-9112c2 into the nose of mice resulted in cns infection as described for wild-type prv. these findings indicate that gb(( ... | 2006 | 16595375 |
| effects of sugar cane extract on pseudorabies virus challenge of pigs. | this experiment aimed to evaluate the efficacy of sugar cane extract (sce) on the modulation of porcine immunity against pseudorabies virus (prv) infection. twelve-week-old experimental pigs were fed with sce (500 mg/kg of body weight per day) for 3 days and challenged with prv (2 x 10(5) tcid(50)) on the second day. pigs that were only challenged with prv and without sce-treatment served as controls. the leukocyte functional assays were performed on the 7th and 14th day post-prv challenge. our ... | 2006 | 16598164 |
| trypsin increases pseudorabies virus production through activation of the erk signalling pathway. | extracellular proteases that are expressed in primary and secondary foci of viral infection are potentially important mediators of infectious inflammatory processes. for some viruses, such as influenza virus and rotaviruses, proteases such as trypsin enhance infectivity by a direct proteolytic effect on some virion proteins. by using an in vitro model of herpesvirus infection, the possibility that proteases modulate the viral cycle through signalling delivered to the infected cell was investigat ... | 2006 | 16603510 |
| a recombinant pseudorabies virus encoding the ha gene from h3n2 subtype swine influenza virus protects mice from virulent challenge. | the hemagglutinin (ha) gene of a/swine/inner mogolian/547/2001 (h3n2) swine influenza virus (siv) was recombined into the genome of pseudorabies virus (prv) bartha-k61 vaccine strain, generating a recombinant prv expressing the ha gene, designated as rprv-ha. one group of 15 mice was inoculated intranasally (i.n.) with 10(5.0) pfu of rprv-ha, and another two control groups of mice (15 mice per group) were mock-inoculated or inoculated with bartha-k61. mice inoculated with rprv-ha developed hemag ... | 2006 | 16621018 |