Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| nucleotide sequence and transcriptional mapping of the major capsid protein gene of pseudorabies virus. | the gene encoding the 142-kda major capsid protein (mcp142) of pseudorabies virus (prv) was isolated and sequenced. nucleotide sequence analysis revealed that the mcp142 gene has a single open reading frame of 3993 nucleotides (nt) encoding 1330 amino acids. the 4400-nt major rna from the mcp142 gene was detected in prv-infected cells. the 5' end of the transcript was located 60 nt upstream of the initiation codon. the 3' end of the transcript was located 18 nt downstream of a putative poly(a) s ... | 1991 | 1718089 |
| computer predictions of antigenic domains in herpes simplex virus types 1 and 2 glycoprotein d as compared with experimentally proven domains. | the primary amino-acid sequence of the glycoprotein d (gd) of herpes simplex virus types 1 and 2 (hsv-1, hsv-2) was analyzed by computer programs that provided values for hydrophilicity, surface probability, flexibility, and antigenicity, as well as the secondary structure conformation. putative antigenic domains with a high hydrophilicity, surface probability, and antigenicity index were determined and compared with the reported antigenic domains in hsv-1 and hsv-2 gd protein based on experimen ... | 1991 | 1724582 |
| severe herpes simplex virus hepatitis following autologous bone marrow transplantation: successful treatment with high dose intravenous acyclovir. | a 17-year-old male patient with t-cell type lymphoblastic lymphoma in complete remission underwent high dose chemotherapy (busulfan 16 mg/kg and cyclophosphamide 120 mg/kg) followed by autologous bone marrow transplantation (abmt). the patient had been taking oral acyclovir (200 mg x 5) daily from seven days prior to the abmt (day -7). on day +24, he complained of epigastralgia and general malaise, and the next day his got and gpt rose to 570 u/l and 397 u/l, respectively. although he had no muc ... | 1991 | 1753418 |
| advantages of multiple cell culture systems for detection of mixed-virus infections. | simultaneous infections by two or more viruses occur frequently, especially in immunosuppressed patients. in order to detect more than one viral agent in a single specimen, multiple cell systems have been employed in our laboratory. specimens are routinely inoculated into four different cell cultures, namely: mrc-5, a human diploid lung fibroblast cell strain; a549, a human continuous cell line; primary guinea pig embryo (gpe) cell culture, and primary rhesus monkey kidney (rhmk) cell culture. f ... | 1991 | 1783674 |
| human cytomegalovirus ie2 negatively regulates alpha gene expression via a short target sequence near the transcription start site. | repression of human cytomegalovirus alpha (immediate-early) gene expression is under the control of the viral ie2 gene. here we show that ie2 negatively regulates gene expression directed by the strong cytomegalovirus enhancer via a specific 15-bp target sequence (which we term cis repression signal [crs]). this crs is located between -14 and +1 relative to the transcription start site and will function in an orientation-independent fashion, consistent with repression occurring at the transcript ... | 1991 | 1846203 |
| transplantation of syngeneic transfected cells to probe the in vivo immune response to viral proteins. | balb/3t3 cells were transfected with the glycoprotein d (gd) gene of herpes simplex virus (hsv) and a cell line expressing gd on the cell surface was isolated. in vitro, 51cr release tests showed that the transfected cells were destroyed by anti-hsv antibody in the presence of complement. to investigate in vivo immune response, the gd-transfected cells were transplanted into the footpads of syngeneic hsv-immunized or unimmunized balb/c mice. in unimmunized mice, transfected cells remained intact ... | 1991 | 1846831 |
| brefeldin a arrests the maturation and egress of herpes simplex virus particles during infection. | herpes simplex virus (hsv) requires the host cell secretory apparatus for transport and processing of membrane glycoproteins during the course of virus assembly. brefeldin a (bfa) has been reported to induce retrograde movement of molecules from the golgi to the endoplasmic reticulum and to cause disassembly of the golgi complex. we examined the effects of bfa on propagation of hsv type 1. release of virions into the extracellular medium was blocked by as little as 0.3 microgram of bfa per ml wh ... | 1991 | 1848309 |
| natural killer-mediated lysis of some but not all hsv-1- or vsv-infected targets requires the participation of hla-dr-positive accessory cells. | natural killer (nk)-mediated lysis of herpes simplex virus type 1-infected fibroblasts (hsv-fs) has been previously shown to require the co-operation of cd16-positive nk cells and an hla-dr-positive accessory cell population. in contrast, lysis of k562 tumour cells requires the presence of only the leu-11-positive cells. in the current study, targets of different morphologies, both virally infected and non-infected, were tested in an attempt to dissect out which target characteristics determine ... | 1991 | 1851136 |
| role of herpes simplex virus thymidine kinase expression in viral pathogenesis and latency. | herpes simplex virus (hsv) thymidine kinase (tk) expression and the hsv tk gene have been evaluated in studies of gene control, as well as in animal and human studies of viral pathogenesis, including hsv latency. in investigations of the biological role of hsv tk, enzyme expression was noted to be important for hsv infection of nonreplicating cells in culture; and, in experimental animal studies, hsv tk was shown to be important for in vivo latent infection of sensory ganglion neurons. latency i ... | 1991 | 1851146 |
| modulation by thiols of the merocyanine 540-sensitized photolysis of leukemia cells, red cells, and herpes simplex virus type 1. | this paper reports on the role of endogenous and exogenous thiols in the merocyanine 540 (mc 540)-sensitized photoirradiation of l1210 leukemia cells, human erythrocytes, and human herpes simplex virus type 1. several measures taken to decrease the intracellular content of glutathione enhanced the cells' sensitivity to mc 540-sensitized photoirradiation while stimulation of glutathione biosynthesis or supplementation of the extracellular or extraviral thiol content decreased the photosensitivity ... | 1991 | 1851305 |
| experimental therapy of human glioma by means of a genetically engineered virus mutant. | malignant gliomas are the most common malignant brain tumors and are almost always fatal. a thymidine kinase-negative mutant of herpes simplex virus-1 (dlsptk) that is attenuated for neurovirulence was tested as a possible treatment for gliomas. in cell culture, dlsptk killed two long-term human glioma lines and three short-term human glioma cell populations. in nude mice with implanted subcutaneous and subrenal u87 human gliomas, intraneoplastic inoculation of dlsptk caused growth inhibition. i ... | 1991 | 1851332 |
| the cytoplasmic tail of hiv-1 gp160 contains regions that associate with cellular membranes. | the hiv-1 envelope glycoprotein gp160 associates with cellular membranes via a discrete transmembrane domain. unlike other retroviral envelope proteins, however, gp160 also forms a secondary association with the lipid bilayer mediated by one or more regions located in the cytoplasmic tail. we have expressed the full cytoplasmic tail sequence of gp160, as a fusion protein with the hsv-1 glycoprotein d signal sequence, transiently in a human embryonic kidney cell line. our results show that in the ... | 1991 | 1984664 |
| s-7 inhibition of herpes simplex virus type 1 infectivity and replication in cultured cells. | the effect of ethyl 2-methylthio-4-methyl-5-pyrimidine, carboxylate (s-7) on the infectivity and replication of herpes simplex virus type 1 in vero cells was studied. s-7, at the highest concentration lacking toxic effect on cells (40 microg/ml), produced, at various inocula, a 40% inhibition of hsv-1 infectivity with respect to the plaque formation detected in control infected cultures. this inhibitory activity was substantiated by studies on progeny production. we cannot conclude that the inhi ... | 1991 | 12041795 |
| promoter-independent activation of heterologous virus gene expression by the herpes simplex virus immediate-early protein icp27. | the herpes simplex virus (hsv) immediate-early protein icp27 has been postulated to play an auxillary role in hsv. gene expression, augmenting or inhibiting the activation of different viral promoters by the other immediate-early proteins icpo and icp4. here we show that icp27 alone can up-regulate gene expression of a retroviral vector containing moloney murine leukemia virus (momulv) regulatory sequences. this is the first report of an effect of icp27 on gene expression driven by heterologous ... | 1992 | 1733102 |
| sequence determination and genetic content of an 8.9-kb restriction fragment in the short unique region and the internal inverted repeat of marek's disease virus type 1 dna. | the dna sequence (8.9 kb) covering about 70% of the short unique region (us) and part of the short inverted repeat of the marek's disease virus type 1 ga strain was determined. computer analysis of the sequence showed the presence of nine potential open reading frames (orfs), consisting of more than 300 nucleotides in the us region. of these orfs, four were found to be homologous to us10 (minor virion protein), us3 (protein kinase), us2, and us6 (gd) in the us region of alpha-herpesvirus herpes ... | 1992 | 1282282 |
| detection of herpesvirus dna in the large intestine of patients with ulcerative colitis and crohn's disease using the nested polymerase chain reaction. | the prevalence of herpesvirus dna was examined in inflammatory bowel disease tissue. dna was extracted from resection and biopsy specimens of the large intestine from patients with ulcerative colitis (n = 21), patients with crohn's disease (n = 29), and patients with noninflammatory bowel disease (controls) (n = 21). the nested polymerase chain reaction was used to detect viral dna using primer pairs specific for either cytomegalovirus (cmv), herpes simplex virus 1 (hsv1), human herpesvirus 6 (h ... | 1992 | 1287131 |
| high levels of enhanced reactivation of herpes simplex virus in skin fibroblasts from various hereditary cancer-prone syndromes. | the dose response of the enhanced reactivation (er) of herpes simplex virus type 1 has been studied in uv-irradiated normal human skin fibroblasts and fibroblasts from the following hereditary cancer-prone syndromes: retinoblastoma, aniridia, polyposis coli, neurofibromatosis type 1 and 2, dysplastic nevus syndrome, von hippel-lindau syndrome, multiple endocrine neoplasia type 2, and bloom's syndrome. surprisingly, much higher levels of er were observed in all these genetically heterogeneous her ... | 1992 | 1309228 |
| the six conserved helicase motifs of the ul5 gene product, a component of the herpes simplex virus type 1 helicase-primase, are essential for its function. | the ul5 protein of herpes simplex virus type 1, one component of the viral helicase-primase complex, contains six sequence motifs found in all members of a superfamily of dna and rna helicases. although this superfamily contains more than 20 members ranging from bacteria to mammalian cells and their viruses, the importance of these motifs has not been addressed experimentally for any one of them. in this study, we have examined the functional significance of these six motifs for the ul5 protein ... | 1992 | 1309257 |
| incorporation of the carbocyclic analog of 2'-deoxyguanosine into the dna of herpes simplex virus and of hep-2 cells infected with herpes simplex virus. | the carbocyclic analog of 2'-deoxyguanosine (cdg) is active against herpes simplex virus (hsv), human cytomegalovirus, and human hepatitis-b virus. in order to understand the mechanism of action of this compound against hsv, we have evaluated (a) the incorporation of [3h]cdg into viral and host dna in hep-2 cells infected with hsv and (b) the interaction of the 5'-triphosphate of cdg (cdg-tp) with the hsv dna polymerase and human dna polymerases alpha, beta, and gamma (ec 2.7.7.7). incubation of ... | 1992 | 1311407 |
| the herpes simplex virus type 1 tegument protein vp22 is encoded by gene ul49. | vp22 is a major tegument protein of herpes simplex virus type 1 and is highly phosphorylated in the infected cell. indirect evidence exists to suggest that it is encoded by gene ul49, present in the bamhi f fragment of the genome. using the polymerase chain reaction we have cloned the ul49 open reading frame into a mammalian expression vector under the control of the human cytomegalovirus immediate early gene promoter. after transfection into cos-7 cells expression of the gene product was detect ... | 1992 | 1312128 |
| protection from retinal necrosis by passive transfer of monoclonal antibody specific for herpes simplex virus glycoprotein d. | passive administration of antibody against herpes simplex virus type 1 (hsv-1) has been shown to protect against stromal keratitis and death from encephalitis. although the exact mechanism by which passively-transferred antibody protects is not known, one of the features of protection by passively-transferred antibody is interference with the ability of the virus to spread within the nervous system. in the experiments reported herein, studies were performed to determine if 8d2, a monoclonal anti ... | 1992 | 1313751 |
| vaginal infection of mice with hsv type 2 variant er-: a new animal model for human primary genital hsv type 2 infections. | studying the pathogenesis of vaginal infections in mice with two variants of herpes simplex virus type 2 (hsv-2) strain er we observed that both variants er+ and er- caused severe vaginitis but only er+ invaded the cns leading to lethal neurological disease. in contrast, mice infected with er- cleared the virus from the vagina and recovered from infection. er+ and er- expressed equal levels of thymidine kinase (tk) indicating a tk-independent difference in neurovirulence. using the non-neuroviru ... | 1992 | 1313825 |
| the gamma 1(34.5) gene of herpes simplex virus 1 precludes neuroblastoma cells from triggering total shutoff of protein synthesis characteristic of programed cell death in neuronal cells. | the gamma 1(34.5) gene of herpes simplex virus 1 was previously shown to play a role in viral virulence since deletion of the gene reduced by a factor of approximately 100,000 the capacity of the virus to replicate in the central nervous system and cause mortality in the mouse. here we show that in the human neuroblastoma cell line sk-n-sh of neuronal origin gamma 1(34.5) null mutants expressed early proteins, viral dna, and mrna of late genes. however, the onset of viral dna synthesis triggered ... | 1992 | 1314384 |
| identification of the 12-o-tetradecanoylphorbol-13-acetate-responsive enhancer of the ms gene of the epstein-barr virus. | we previously located two 12-o-tetradecanoylphorbol-13-acetate (tpa)-responsive enhancers, mstre-i and mstre-ii, in the upstream sequence of the ms gene of epstein-barr virus (liu, q., and summers, w.c. (1989) j. virol. 63, 5062-5068). the core sequence of the mstre-i enhancer is now determined to be between -718 and -708 of the upstream sequence of the ms gene. the activity of the enhancer is also sensitive to its immediate surrounding sequence on either side. a single copy of a 30-base pair (b ... | 1992 | 1318308 |
| recombination between a herpes simplex virus type 1 vector deleted for immediate early gene 3 and the infected cell genome. | we have used a vector derived from a herpes simplex virus type 1 (hsv-1) mutant deleted for 3.6 kbp of the essential immediate early gene 3 to transduce the tn5 neomycin phosphotransferase (neor) gene into rodent and primate cells in culture. the transgene was flanked by genomic sequences from the human hprt gene. we demonstrate in this study that sequences introduced by infection with the replication-defective hsv vector can be stably inserted into the cell genome by recombination. both the eff ... | 1992 | 1318949 |
| identification of the glycoprotein h gene of murine cytomegalovirus. | partial sequencing of the hindiii c fragment of murine cytomegalovirus (mcmv) revealed an open reading frame of 2172 nucleotides in length encoding a 724 amino acid protein with a predicted mr of 80.4k. analysis of the predicted amino acid sequence revealed homology with glycoprotein h (gh) from a number of other herpesviruses. mcmv gh showed strongest amino acid identity with human (h) cmv and human herpesvirus 6 gh, and less identity with the gh protein sequences of epstein-barr virus, varicel ... | 1992 | 1321219 |
| a novel herpes simplex virus gene (ul49a) encodes a putative membrane protein with counterparts in other herpesviruses. | comparative analysis of dna sequences located between the coding regions of genes ul49 and ul50 of herpes simplex virus types 1 and 2 (hsv-1 and -2) has revealed a small open reading frame (orf) of 91 and 87 codons respectively with the characteristics of a genuine protein-coding region. the predicted protein products are clearly related and exhibit features of membrane-inserted proteins, with potential n-proximal signal peptides and c-proximal membrane anchor regions. counterparts are present i ... | 1992 | 1322965 |
| the herpes simplex virus type 1 immediate-early polypeptide icp4 is required for expression of globin genes located in the viral genome. | we infected vero cells with icp4-deficient herpes simplex virus recombinants bearing the rabbit beta-globin and human alpha 2-globin genes under the control of their own promoters and found that globin gene expression occurred only when icp4 was provided in trans. these results demonstrate that icp4 is required for the activity of globin promoters located in the viral genome and support the hypothesis that these cellular promoters are functionally equivalent to hsv early regulatory regions. | 1992 | 1326818 |
| sequences of the bovine herpesvirus 1 homologue of herpes simplex virus type-1 alpha-trans-inducing factor (ul48). | a virion protein of herpes simplex virus type-1, called vmw65, alpha tif or vp16, interacts with cellular transcription factors to transactivate immediate early viral genes. we have cloned and determined the nucleotide sequence of the gene encoding the homologous protein in bovine herpesvirus 1 (bhv-1). the amino acid sequence of the bhv-1 protein is similar to that of alpha tif, except in the c-terminal one-third of the protein. since the ability of alpha tif to activate transcription is depend ... | 1992 | 1327963 |
| glycoprotein h of human cytomegalovirus (hcmv) forms a stable complex with the hcmv ul115 gene product. | the human cytomegalovirus (hcmv) ul75 gene product is the homologue of herpes simplex virus type 1 (hsv-1) glycoprotein h (gh), a virion glycoprotein that is essential for infectivity and which is conserved among members of the alpha-, beta- and gamma-herpesviruses. it has previously been shown that hsv-1 gh forms a stable complex with hsv-1 gl, the product of the ul1 gene, and the formation of this complex facilitates the cell surface expression of gh. none of the open reading frames within the ... | 1992 | 1328481 |
| the conserved helicase motifs of the herpes simplex virus type 1 origin-binding protein ul9 are important for function. | the ul9 gene of herpes simplex virus encodes a protein that specifically recognizes sequences within the viral origins of replication and exhibits helicase and dna-dependent atpase activities. the specific dna binding domain of the ul9 protein was localized to the carboxy-terminal one-third of the molecule (h. m. weir, j. m. calder, and n. d. stow, nucleic acids res. 17:1409-1425, 1989). the n-terminal two-thirds of the ul9 gene contains six sequence motifs found in all members of a superfamily ... | 1992 | 1328687 |
| comparison of immunocytology to tissue culture for diagnosis of presumed herpesvirus dendritic epithelial keratitis. | the objective of this study is to prospectively compare the sensitivity and specificity of immunodetection of herpes simplex virus (hsv) in impression cytology specimens obtained directly from presumed herpesvirus dendritic epithelial keratitis with virus isolation by tissue culture of cells scraped from the same lesion. | 1992 | 1328982 |
| transcription of bamhi-a region of the ebv genome in npc tissues and b cells. | analysis by northern blotting and sequencing of cdna clones from a transcription library of a tumor biopsy from a nasopharyngeal carcinoma (npc) patient showed that the bamhi-a region of the epstein-barr virus genome is abundantly and regularly transcribed in tumor tissues from npc patients. the transcription occurred in a rightward direction terminating between coordinates 160,965 and 160,995, where two polyadenylation sites are located. rightward transcription of this region also occurred in b ... | 1992 | 1329317 |
| l-thymidine is phosphorylated by herpes simplex virus type 1 thymidine kinase and inhibits viral growth. | we have demonstrated that herpes simplex 1 (hsv1) thymidine kinase (tk) shows no stereospecificity for d- and l-beta-nucleosides. in vitro, l enantiomers are not recognized by human tk, but function as specific substrates for the viral enzyme in the order: l-thymidine (l-t) >> 2'-deoxy-l-guanosine (l-dg) > 2'-deoxy-l-uridine (l-du) > 2'-deoxy-l-cytidine (l-dc) > 2'-deoxy- l-adenosine (l-da). hsv1 tk phosphorylates both thymidine enantiomers to their corresponding monophosphates with identical ef ... | 1992 | 1331461 |
| analysis of the ul36 open reading frame encoding the large tegument protein (icp1/2) of herpes simplex virus type 1. | using peptide antisera specific for regions within the n terminus and c terminus of the predicted ul36 gene product, immunoblotting experiments were performed to demonstrate definitively that icp1/2 is encoded by the ul36 gene. these data also suggest that both the cell- and the virion-associated forms of icp1/2 are colinear with the complete predicted amino acid sequence of the ul36 gene. computer-assisted analyses of the predicted amino acid sequence of the ul36 gene revealed the presence of t ... | 1992 | 1331541 |
| effects of dimethyl prostaglandin a1 on herpes simplex virus and human immunodeficiency virus replication. | we have investigated the direct effect of dimethyl prostaglandin a1 (dmpga1) on the replication of herpes simplex virus (hsv) and human immunodeficiency virus type 1 (hiv-1). dmpga1 significantly inhibited viral replication in both hsv and hiv infection systems at concentrations of dmpga1 that did not adversely alter cellular dna synthesis. the 50% inhibitory concentration (id50) for several hsv type 1 (hsv-1) strains ranged from 3.8 to 5.6 micrograms/ml for vero cells and from 4.6 to 7.3 microg ... | 1992 | 1332592 |
| improved synthesis and biological evaluation of an acyclic thiosangivamycin active against human cytomegalovirus. | we previously described the synthesis and in vitro antiviral activity of an acyclic thiosangivamycin analog (gupta et al., 1989a). in order to extend these initial studies, a new, multi-gram synthesis of 4-amino-7-[(2-hydroxy- ethoxy)methyl]pyrrolo]2,3-d]pyrimidine-5-thiocarboxamide (compound 229) was achieved in 5 steps from the known 5-amino-2-bromo-3,4-dicyanopyrrole in good overall yield. in plaque reduction assays with hcmv, compound 229 had an ic50 of 7 microm; in yield reduction assays th ... | 1992 | 1332597 |
| rapid detection of human herpes simplex virus type 1 in saliva. | herpes simplex virus type 1 (hsv1) can be rapidly identified in saliva from patients with acute herpetic gingivostomatitis, by in vitro amplification using the polymerase chain reaction and specific primers. amplification of dna results in a product of 110 bp length corresponding to the region 1381-1490 bp of the hsv1 thymidine kinase gene. the specificity of the reaction was demonstrated in three ways: (i) the presence of a sma 1 restriction enzyme site in the amplified product sequence; (ii) s ... | 1992 | 1332660 |
| replication inhibition by nucleoside analogues of a recombinant autographa californica multicapsid nuclear polyhedrosis virus harboring the herpes thymidine kinase gene driven by the ie-1(0) promoter: a new way to select recombinant baculoviruses. | the expression of the thymidine-thymidylate kinase (hsv1-tk), (atp: thymidine 5'-phosphotransferase; ec 2.7.1.21) of herpes simplex virus type 1 endows the host cell with a conditional lethal phenotype which depends on the presence of nucleoside analogues metabolized by this enzyme into toxic inhibitors of dna replication. to generate a recombinant baculovirus that could be selected against by nucleoside analogs, the hsv1-tk coding sequence was placed under the control of the autographa californ ... | 1992 | 1335569 |
| detection of latent herpes simplex virus dna and rna in human geniculate ganglia by the polymerase chain reaction. | by using the polymerase chain reaction (pcr) we detected latent herpes simplex virus type 1 (hsv-1) in human geniculate and trigeminal ganglia obtained from autopsy cases. a pair of primers which were specific for a part of the hsv-1 thymidine kinase domain were used for detection of hsv dna. we also examined the latency-associated transcript (lat), known as latency-specific rna, by means of reverse transcription-pcr with a pair of lat-specific primers. hsv-1 dna was detected in 16 of 17 (94%) t ... | 1992 | 1336296 |
| search for evidence of herpes simplex virus, type 1, or varicella-zoster virus infection in postmortem brain tissue from schizophrenic patients. | the highly sensitive polymerase chain reaction (pcr) was used to search for herpes simplex virus type 1 (hsv-i) or varicella-zoster virus (vzv) in the dna extracted from postmortem temporal cortex samples of 8 schizophrenic subjects, 8 nonschizophrenic suicide victims and 8 normal controls. hsv-i or vzv-specific dna amplification was not detected in any of the samples studied. | 1992 | 1336636 |
| establishment and functional characterization of human herpesvirus 6-specific cd4+ human t-cell clones. | in order to clarify the protective immune responses against a newly identified herpesvirus, human herpesvirus 6 (hhv-6), we established hhv-6-specific human t-cell clones and examined their functional properties. five cd3+cd4+cd8- t-cell clones, which proliferated in response to stimulation with two different strains of hhv-6 in the presence of autologous antigen-presenting cells but not with herpes simplex virus type 1 or human cytomegalovirus, were established from peripheral blood lymphocytes ... | 1992 | 1348547 |
| inhibition of hsv-1-specific cytotoxic t lymphocytes by recombinant-derived gp120 of hiv-1. | the ability of human immunodeficiency virus type-1 (hiv-1) and recombinant hiv-1 gp120 to prevent target cell lysis by herpes simplex virus type 1 (hsv-1)-specific cytotoxic t lymphocytes (ctl) was assessed by limiting dilution analysis. live and inactivated hiv-1 as well as recombinant-derived gp120 all substantially inhibited hsv-1-specific ctl. soluble cd4 antigen reversed the inhibition by gp120 when simultaneously added with gp120 to the assay. in addition, the monoclonal anti-cd4 antibody ... | 1992 | 1361737 |
| differential mapping of fc gamma-binding and monoclonal antibody-reactive epitopes on ge, the fc gamma-binding glycoprotein of herpes simplex virus type 1. | the entire 396 residue extracellular sequence of ge the hsv-1 fc gamma-binding glycoprotein has been studied to determine epitopes binding to two mab ii-481 and 88s previously demonstrated to react with ge at or near the fc gamma-binding regions. overlapping 7-mers constructed from the established sequence were tested with mab ii-481 and 88s along with their fab fragments. control mab of the same igg 2b subclass as well as whole rabbit and human igg and fc were also tested for binding to overlap ... | 1992 | 1382102 |
| mutagenesis by apurinic sites in normal and ataxia telangiectasia human lymphoblastoid cells. | we used a shuttle vector based on the epstein-barr virus origin of plasmid replication (orip) to determine the types of mutations induced by depurination in human cells. plasmid dna was incubated at ph 2 at 40 degrees c for various times to induce up to 20 apurinic (ap) sites per 9.7-kb plasmid and electroporated into lymphoblastoid cells derived from either a normal individual or an ataxia telangiectasia patient. after replication of the vector in the human cells, plasmid dna was isolated and a ... | 1992 | 1503643 |
| report of twelve years experience in open study of skinner herpes simplex vaccine towards prevention of herpes genitalis. | three hundred and forty-seven subjects at risk for herpes genitalis were vaccinated with skinner vaccine, nfuac.hsv1.(s-mrc5), and were followed for an average duration of 2 years representing a total consortship of 664.4 years. based on survey information obtained during this consortship, there were estimated to be 3076 recurrences which summated to 3.5 years total duration of disease and comprised at least 6794 lesions; there were an estimated 51997 episodes of intercourse including at least 2 ... | 1992 | 1549071 |
| acyclovir bioavailability in human skin. | clinical experience demonstrates that oral acyclovir (acv) is superior to topical acv in treating recurrent cutaneous herpes simplex virus type 1 (hsv-1) infections. cutaneous hsv-1 infections are complex in their pathology, affecting the basal epidermis in skin as well as establishing a latency phase in sensory ganglia. in vitro and in vivo human skin model systems were used in the present study to quantitate acv disposition and absorption in skin and blood following two routes of administratio ... | 1992 | 1593149 |
| herpes simplex virus immediate early gene expression in the absence of transinduction by vmw65 varies during the cell cycle. | the requirement for the herpes simplex virus type 1 (hsv-1) protein vmw65 (vp16) for activation of immediate early (ie) gene expression was examined in synchronized hela cells. analyses of ie rna levels were conducted during infection with a viral vmw65 mutant, in1814. the results revealed an increased requirement for vmw65 when cultures reached g2 phase of the cell cycle. the levels of ie rnas 1, 2, and 4 were reduced 5-10 times more in g2 than g1/s for in1814-infected cells when compared to ce ... | 1992 | 1604810 |
| merocyanine-sensitized photoinactivation of enveloped viruses. | a wide range of enveloped viruses, including human herpes simplex virus type 1, human cytomegalovirus, human t cell leukemia/lymphoma virus type i, human immunodeficiency virus type 1, sindbis virus, and friend erythroleukemia virus, are highly susceptible to merocyanine 540 (mc 540)-sensitized photoinactivation. by contrast, human pluripotent hematopoietic stem cells, red cells, factor viii, and von willebrand factor are much less sensitive. this suggests that mc 540 may be useful for the inact ... | 1992 | 1617187 |
| use of isotopically chiral [4'-13c]penciclovir and 13c nmr to determine the specificity and absolute configuration of penciclovir phosphate esters formed in hsv-1 and hsv-2 infected cells and by hsv-1-encoded thymidine kinase. | penciclovir is a potent antiherpesvirus agent which is highly selective due to its phosphorylation only in virus infected cells. phosphorylation of one of the hydroxymethyl groups of penciclovir (pcv) creates a chiral centre leading to the possible formation of (r)- and (s)-enantiomers. the absolute configuration and stereospecificity of the pcv-phosphates produced in cells infected with herpes simplex viruses types 1 and 2 (hsv-1 and hsv-2), as well as by hsv-1-encoded thymidine kinase, were de ... | 1993 | 8305285 |
| size dependent variation in the fractal dimensions of herpes simplex epithelial keratitis. | dendritic ulcers, the commonest manifestation of herpes simplex epithelial keratitis (hsek) are fractals. it is likely that their fractal properties alter if they progress to a geographic appearance. this study investigates the relationship of maximum ulcer diameter (feret's diameter) to area (dm) and perimeter fractal dimensions (ds), parameters of the complexity of their areas and outlines respectively. for dendritic ulcers in the size range of 1.6-3.2 mm, dm = 1.41 +/- 0.06 and ds = 1.40 +/- ... | 1993 | 8306714 |
| the herpes simplex virus type i origin binding protein. dna-dependent nucleoside triphosphatase activity. | a recombinant baculovirus overexpressing the herpes simplex virus type 1 (hsv-1) origin binding protein, encoded by the ul9 gene, was constructed. the purified recombinant protein has dna-dependent nucleoside triphosphatase activity similar to the enzyme isolated from mammalian cells. optimal nucleoside triphosphatase activity requires low salt (< 50 mm), 2-3 mm mg2+, alkaline ph (8.3-9.5), high temperature (45 degrees c), and a single-stranded dna coeffector containing minimal secondary structu ... | 1993 | 8380407 |
| nf-kappa b-binding proteins induced by hsv-1 infection of u937 cells are not involved in activation of human immunodeficiency virus. | molecular interactions between the herpes simplex virus type 1 (hsv-1) and human immunodeficiency virus (hiv) were investigated in u937 cells. nonpermissive hsv-1 infection of u937 cells activated hiv as determined in transient expression assays with hybrid constructions containing the hiv-ltr-directed chloramphenicol acetyltransferase gene. activation was independent of kappa b-enhancer elements whereas these elements were required for hsv-1-mediated activation in another cell line (c6). kappa ... | 1993 | 8380662 |
| mutations in herpes simplex virus type 1 genes encoding vp5 and vp23 abrogate capsid formation and cleavage of replicated dna. | the herpes simplex virus type 1 capsid is composed of seven capsid proteins which are termed vp5, vp19c, vp21, vp22a, vp23, vp24, and vp26. major capsid protein vp5 is encoded by the gene ul19. ul18, whose transcript is 3' coterminal with that of vp5, specifies capsid protein vp23. vero cell lines have been isolated that are transformed with either the bglii n (ul19) or ecori g (ul16 to ul21) fragment of kos. these cell lines, selected for the ability to support the replication of a temperature- ... | 1993 | 8382300 |
| hsv-1 vector-mediated gene transfer of the human nerve growth factor receptor p75hngfr defines high-affinity ngf binding. | a series of recombinant herpes simplex virus (hsv-1) vectors have been constructed that encode either the full-length cdna of the human p75 ngf receptor (p75hngfr) or truncated forms of the receptor. infection of cultured fibroblast cells with viral stocks results in abundant expression of all three cdnas, as detected by affinity cross-linking, immunoblot analysis, and equilibrium binding. furthermore, viral infection of primary neuronal cultures gives easily detectable p75 expression by immunof ... | 1993 | 8382740 |
| the herpes simplex virus type 1 us11 gene product is a phosphorylated protein found to be non-specifically associated with both ribosomal subunits. | microsequencing of a cyanogen bromide peptide obtained from a basic phosphoprotein co-sedimenting with purified ribosomes extracted from herpes simplex virus type 1-infected human epidermoid carcinoma 2 cells identified this protein as a product of the true late us11 gene. an antibody was raised against a recombinant fusion protein expressed in escherichia coli from a plasmid carrying 75% of the us11 coding sequence including the carboxy terminus. this antibody was used to probe western blots ca ... | 1993 | 8383175 |
| the pseudorabies virus host-shutoff homolog gene: nucleotide sequence and comparison with alphaherpesvirus protein counterparts. | the virion host shutoff function (vhs) of many herpesviruses is required for inhibition of cellular gene expression in infected cells. this function corresponds to the ul41 open reading frame of herpes simplex virus type 1 (hsv-1) and homolog sequences have been found in other alphaherpesvirus, like hsv-2, varicella-zoster virus (vzv) and equine herpesvirus type 1 (ehv-1). in this work, we have cloned and sequenced a pseudorabies virus (prv) gene which is homologous to the vhs genes of the other ... | 1993 | 8384744 |
| purification and properties of the herpes simplex virus type 1 ul8 protein. | a rapid and simple two-step scheme for the purification of herpes simplex virus type 1 ul8 protein from insect cells infected with a recombinant baculovirus has been developed. the scheme involves deae-sepharose and phenyl-sepharose chromatography and yields approximately 1.5 mg of protein from 2.4 x 10(8) infected cells. the protein remains intact during purification as judged by its reactivity with amino and carboxy termini-specific antisera. gel filtration chromatography showed that the prote ... | 1993 | 8385691 |
| the ggtca palindrome and cognate cellular factors in trans-regulation of human immunodeficiency virus long terminal repeat by herpes simplex virus. | molecular interactions between herpes simplex virus type 1 (hsv-1) and human immunodeficiency virus (hiv) were investigated in the promonocytic cell line u937. hsv-1-mediated activation was observed in transient expression assays with hybrid constructions containing the hiv long terminal repeat (ltr)-directed chloramphenicol acetyltransferase gene. comparison of constructions that differ in the ggtca palindrome located within the negative regulatory region of the ltr revealed four- to eightfold ... | 1993 | 8385697 |
| induction of bilateral retinal necrosis in mice by unilateral intracameral inoculation of a glycoprotein-c deficient clinical isolate of herpes simplex virus type 1. | herpes simplex virus can cause acute retinal necrosis, a blinding retinal disease in man. a unilateral intracameral inoculation of herpes simplex virus type 1 (hsv-1) in mice induces retinal necrosis primarily in the contralateral eye and provides an experimental model for the disease. previous studies suggested that a major envelope glycoprotein of hsv-1, glycoprotein c (gc), is required for retinal necrosis. we studied hsv-1 strain tn-1, a gc-deficient clinical isolated from a lesion of herpet ... | 1993 | 8385909 |
| varicella-zoster virus open reading frame 10 protein, the herpes simplex virus vp16 homolog, transactivates herpesvirus immediate-early gene promoters. | the varicella-zoster virus (vzv) open reading frame 10 (orf10) protein is the homolog of the herpes simplex virus type 1 (hsv-1) protein vp16. these are two virion tegument proteins that have extensive amino acid sequence identity in their amino-terminal and middle domains. orf10, however, lacks the acidic carboxy terminus which is critical for transactivation by vp16. earlier studies showed that vzv orf10 does not form a tertiary complex with the taatgarat regulatory element (where r is a purin ... | 1993 | 8386275 |
| altering central nervous system physiology with a defective herpes simplex virus vector expressing the glucose transporter gene. | because of their postmitotic nature, neurons are difficult subjects for gene transfer. to circumvent this, we have used a defective herpes simplex virus vector to overexpress the rat brain glucose transporter (gt) gene under the control of the human cytomegalovirus ie1 promoter. this vector, designated vie1gt, was propagated using a herpes simplex virus type 1 temperature-sensitive mutant, ts756. gt expressed from vie1gt was readily immunoprecipitated from membrane fractions of vie1gt-infected v ... | 1993 | 8386379 |
| in vitro antiviral activity of polyoxomolybdates. mechanism of inhibitory effect of pm-104 (nh4)12h2(eu4(moo4)(h2o)16(mo7o24)4).13h2o on human immunodeficiency virus type 1. | a screening for inhibitors of human immunodeficiency virus type 1 (hiv-1) among various types of isopolyoxomolybdates and heteropolyoxomolybdates was carried out by using an in vitro assay system measuring the cytopathogenicity of hiv-1 in cd4+ human mt-4 cells. a novel heteropolyoxomolybdate named pm-104 with the chemical formula (nh4)12h2(eu4(moo4)(h2o)16(mo7o24)4).13h2o was found to be associated with potent anti-hiv-1 activity. pm-104 interferes with virus infection at a very early step such ... | 1993 | 8387260 |
| intranasal immunization against herpes simplex virus infection by using a recombinant glycoprotein d fused with immunomodulating proteins, the b subunit of escherichia coli heat-labile enterotoxin and interleukin-2. | to establish a novel strategy of mucosal immunization against herpes simplex virus type 1 (hsv-1) infection, we studied the immune responses elicited by intranasal immunization with several forms of a recombinant glycoprotein d (gd) of hsv-1. a truncated gd (t-gd) co-administered with heat-labile enterotoxin b subunit (ltb) from escherichia coli induced both a mucosal immune response involving secretion of anti-gd iga and serum igg production. the levels of these responses are comparable to thos ... | 1993 | 8388365 |
| direct identification of herpes simplex virus type-1 in human corneal tissue. methodology and application of in situ dna hybridization technique. | the definitive diagnosis and determination of recurrence of herpes simplex keratitis are still difficult in clinical ophthalmology. at present, isolation of virus by tissue culture is perhaps the best method for establishing a specific aetiological diagnosis of viral infection. but due to its complicated and time-consuming procedures, the application of tissue culture for virus isolation in clinical work is still limited. in situ dna hybridization is a specific and quick technique for directly d ... | 1993 | 8389272 |
| age distribution of latent herpes simplex virus 1 and varicella-zoster virus genome in human nervous tissue. | latency in nervous tissue caused by herpes simplex virus 1 (hsv-1) and by varicella-zoster virus (vzv) is an intriguing feature of herpes-virus' neurotropism. hsv-1 and vzv latency are the causes of ophthalmic zoster and recurrent hsv infections in the distributions of the trigeminal branches. hsv-1 neuronal latency may play a role in the etiopathogenesis of hsv encephalitis. we attempted to determine the prevalence and age distribution of vzv and hsv latency. we applied nested polymerase chain ... | 1993 | 8389816 |
| varicella-zoster virus (vzv) open reading frame 61 protein transactivates vzv gene promoters and enhances the infectivity of vzv dna. | the varicella-zoster virus (vzv) open reading frame 61 (orf61) protein is the homolog of herpes simplex virus type 1 (hsv-1) icp0. both genes are located in similar parts of the genome, their predicted products share a cysteine-rich motif, and cell lines expressing vzv orf61 are able to complement an hsv-1 icp0 deletion mutant (h. moriuchi, m. moriuchi, h. a. smith, s. e. straus, and j. i. cohen, j. virol. 66:7303-7308, 1992). in transient expression assays, hsv-1 icp0 is a transactivator alone ... | 1993 | 8389928 |
| antineoplastic and antiviral activities of some cyclolignans. | nineteen cyclolignans of varied structures, most of them isolated from juniperus sabina leaves, were evaluated for their antineoplastic and antiviral activities. they were subjected to screening against p-388 murine leukemia, a-549 human lung carcinoma, and ht-29 colon carcinoma, while the antiviral assays were performed on herpes simplex virus type 1 infecting fibroblasts of monkey kidney (hsv-1/cv-1) and on vesicular stomatitis virus infecting fibroblasts of hamster kidney (vsv/bhk). a number ... | 1993 | 8391145 |
| identification of the gene product encoded by orf ul56 of the human cytomegalovirus genome. | experiments were undertaken to identify the product of open reading frame (orf) ul56 of human cytomegalovirus (hcmv), the putative homolog of infected cell protein (icp) 18.5 of herpes simplex virus type 1 (hsv-1) that is thought to be involved in viral nucleocapsid maturation. northern blotting using a unique fragment of orf ul56 revealed a specific transcript of about 3.0 kb in hcmv-infected fibroblasts early and late postinfection (p.i.). two overlapping fragments of ul56 were subcloned for p ... | 1993 | 8395117 |
| inhibition of herpes simplex virus type 1 dna replication by mutant forms of the origin-binding protein. | the herpes simplex virus type 1 (hsv-1) origin-binding protein (obp) is a sequence-specific dna binding protein encoded by gene ul9 which interacts with the viral origins of dna replication and also exhibits dna helicase activity. sequence-specific dna binding activity has previously been shown to reside within the c-terminal 317 amino acids, and when expressed alone, this domain exerts a dominant inhibitory effect on hsv-1 dna synthesis. we have tested several ul9 gene mutants for ability to su ... | 1993 | 8396795 |
| [double meningoencephalitis with herpes simplex virus and rubella virus]. | a 47-year-old man suffered from a headache, fever and memory disturbance. he was admitted to morimoto hospital. neurological examination revealed disturbance of memory of recent events. cranial nerves were normal. muscle strength of the extremities was normal, except for barré's sign of the right upper extremity. deep tendon reflexes were exaggerated bilaterally, and extensor plantar response of the left side was elicited. sensory examination showed no abnormality. cerebellar sign was not recogn ... | 1993 | 8403685 |
| herpes simplex virus glycoprotein c: molecular mimicry of complement regulatory proteins by a viral protein. | herpes simplex virus (hsv) encodes a protein, glycoprotein c (gc), which binds to the third complement component, the central mediator of complement activation. in this study the structural and functional relationships of gc from hsv type 1 (hsv-1) and known human complement regulatory proteins factor h, properdin, factor b, complement receptor 1 (cr1) and 2 (cr2) were investigated. the interaction of gc with c3b was studied using purified complement components, synthetic peptides, antisera agai ... | 1993 | 8406590 |
| an evaluation of oral ulcers in patients with aids and aids-related complex. | patients with hiv infection can have recurrent and persistent oral ulcers, not attributable to known infectious agents. | 1993 | 8408791 |
| [from herpes to zona]. | 1993 | 8410632 | |
| antiviral activity of c-alkylated purine nucleosides obtained by cross-coupling with tetraalkyltin reagents. | 2-, 6-, and 8-alkylated (methyl, ethyl, and vinyl) adenosine analogues were synthesized by a palladium-catalyzed cross-coupling of a tetraalkyltin with the halogenated purine nucleosides. the synthesis of the 8-substituted analogues was accomplished using a transient protection procedure. the 6-alkylated-9-beta-d-ribofuranosylpurines as well as 2-ethyladenosine were cytotoxic at relatively low concentrations (0.8-10 micrograms/ml). 8-methyladenosine was a potent and selective inhibitor of vaccin ... | 1993 | 8411010 |
| inhibition of herpes simplex virus type 1 ribonucleotide reductase by substituted tetrapeptide derivatives. | it is known that peptides corresponding to the c-terminus of the small subunit of herpes simplex virus type 1 and 2 ribonucleotide reductase can inhibit enzymatic activity by preventing the association of the enzyme's two subunits. in a quest for smaller, more potent inhibitors, we have conducted a structure activity investigation based on the pentapeptide h-val-val-asn-asp-leu-oh. potency increases of up to 4000 times (ic50 0.18 microm) have been achieved in an enzymatic assay by a combination ... | 1993 | 8411018 |
| differential antiherpesvirus and antiretrovirus effects of the (s) and (r) enantiomers of acyclic nucleoside phosphonates: potent and selective in vitro and in vivo antiretrovirus activities of (r)-9-(2-phosphonomethoxypropyl)-2,6-diaminopurine. | the (s)- and (r)-enantiomers of acyclic purine nucleoside phosphonate analogs (i.e., 3-hydroxy-2-phosphonomethoxypropyl [hpmp] derivatives, 3-fluoro-2-phosphonomethoxypropyl [fpmp] derivatives, and 2-phosphonomethoxypropyl [pmp] derivatives of adenine [a], 2-aminopurine, 2,6-diaminopurine [dap], and guanine [g]) have been synthesized and evaluated for antiviral activity. as a rule, the hpmp derivatives proved effective against dna viruses but not rna viruses or retroviruses. in particular, (s)-h ... | 1993 | 8452366 |
| novel acyclonucleotides: synthesis and antiviral activity of alkenylphosphonic acid derivatives of purines and a pyrimidine. | a series of phosphonoalkenyl and (phosphonoalkenyl)oxy derivatives of purines and a pyrimidine were synthesized. these compounds are the first reported acyclonucleotides which incorporate the alpha,beta-unsaturated phosphonic acid moiety as the phosphate mimic and include compounds in which the acyclic substituent is attached to n-9 of a purine or n-1 of a pyrimidine by either a nitrogen-carbon or a nitrogen-oxygen bond. the phosphonoalkenyl-substituted compounds 7a-c, 8a-c, 9, 10, and 12 were p ... | 1993 | 8496903 |
| a novel arrangement of zinc-binding residues and secondary structure in the c3hc4 motif of an alpha herpes virus protein family. | a highly conserved, cysteine-rich region plays a crucial role in the function of a family of regulatory proteins encoded by alpha herpes viruses. the so-called c3hc4 motif spans approximately 60 residues and has been predicted to bind zinc. this motif occurs in a number of other viral and cellular proteins, many of which appear to be involved in some aspect of the regulation of gene expression. we have cloned and expressed in bacteria a portion of immediate-early protein vmw110 of herpes simplex ... | 1993 | 8263911 |
| [detection of herpes simplex virus 1 and 2 (hsv-1 and hsv-2) igg and igm by elisa in cord blood and sera of mothers with pregnancy complications]. | in this study, hsv-1 igg, igm and hsv-2 igg, igm antibodies by elisa in the sera of the mothers who had different kinds of obstetrical problems like abortus, stillbirth, prematurity, postmaturity, intrauterine development retardation and in the newborns' cord sera who had congenital anomalies like anencephaly, cataract and dolichocephaly were investigated. in these mothers hsv-1 igg positivity ratio was 71/73 (97.3%). there was no significant difference in the age group distribution of hsv-1 igg ... | 1993 | 8264443 |
| acyclovir diphosphate dimyristoylglycerol: a phospholipid prodrug with activity against acyclovir-resistant herpes simplex virus. | infection with herpes simplex viruses (hsvs) resistant to treatment with acyclovir (9-[(2-hydroxyethoxy)-methyl]guanine, zovirax) is a growing clinical problem in patients with aids and other immunosuppressed states. most virus isolates resistant to acyclovir are deficient or defective in virally coded thymidine kinase (tk), which converts acyclovir to acyclovir monophosphate in virus-infected cells. to restore acyclovir efficacy, we synthesized acyclovir diphosphate dimyristoylglycerol, an anal ... | 1993 | 8265634 |
| production of herpes simplex virus fluorescein labelled typing reagents. | 29 herpes simplex virus monoclonal antibodies (mabs) were produced and shown by indirect immunofluorescence assay (iifa) to be hsv type specific or hsv cross reactive. two hsv-1 and two hsv-2 specific mabs were selected as hsv typing reagents on the basis of their specificity and the fluorescence pattern produced. when directly conjugated to fluorescein both reagents detected and correctly identified hsv isolates previously typed by restriction endonuclease analysis (rea). no cross reactions wer ... | 1993 | 8270652 |
| modification of discrete nuclear domains induced by herpes simplex virus type 1 immediate early gene 1 product (icp0). | the outcome of herpes simplex virus type 1 (hsv-1) infection depends upon the interplay of both host and viral factors. during lytic infection, hsv-1 causes a loss of immunofluorescent staining of discrete nuclear domains (nd10). this elimination of the host's nd10 staining occurs under conditions that allow only hsv-1 immediate early viral gene expression. western blot analysis indicates that the loss of nd10 staining is due to nd10 redistribution, rather than protein degradation or turnover. w ... | 1993 | 8277273 |
| analysis of protective immune responses to the glycoprotein h-glycoprotein l complex of herpes simplex virus type 1. | a recombinant vaccinia virus expressing both glycoprotein h (gh) and glycoprotein l (gl) of herpes simplex virus type 1 (hsv-1) was used to examine the protective response to gh-gl in immunized mice and to compare these responses with those induced by the highly protective immunogen, glycoprotein d (gd). weak levels of hsv-1-specific neutralizing antibody were obtained in response to the gh-gl complex, virus clearance from the site of challenge was marginally enhanced compared to that observed f ... | 1993 | 8277292 |
| antiviral traditional medicines against herpes simplex virus (hsv-1), poliovirus, and measles virus in vitro and their therapeutic efficacies for hsv-1 infection in mice. | one hundred forty-two kinds of traditional medicines, which have been historically used in china, indonesia, and japan, were examined for the antiviral activity of their hot water (hw) extracts against herpes simplex virus type 1 (hsv-1), poliovirus type 1, and measles virus by plaque reduction assay. thirty-two, 55, and 30 hw-extracts of them showed anti-hsv-1, antipoliovirus, and anti-measles virus activities, respectively. among the 32 hw-extracts with anti-hsv-1 activity, 3 hw-extracts had a ... | 1993 | 8279811 |
| isolation and structure of woodorien, a new glucoside having antiviral activity, from woodwardia orientalis. | hot aqueous and methanol extracts of the rhizomes of woodwardia orientalis were tested for their in vitro antiviral activity against herpes simplex virus type 1 (hsv-1), poliovirus type 1, and measles virus by plaque reduction assay. the aqueous extract of w. orientalis reduced the plaque forming ability of hsv-1 and poliovirus more strongly than did the methanol extract. by bioassay-directed fractionation of the aqueous extract, a new glucoside, woodorien (1), along with five known compounds we ... | 1993 | 8281578 |
| specific transcriptional activation in vitro by the herpes simplex virus protein vp16. | the herpes simplex virus protein vp16 interacts with cellular factors, including the protein oct-1, to activate viral immediate early (ie) gene transcription. we have reproduced this effect by addition of purified, full-length vp16 and the dna-binding 'pou' domain of oct-1 (oct-1/pou) to a hela cell in vitro transcription system. stimulation of transcription was dependent on the ie-specific element, taatgarat. in agreement with earlier observations from electrophoretic mobility shift assays, act ... | 1993 | 8284200 |
| two novel genes of herpes simplex virus type 1 involved in cell fusion. | herpes simplex virus type 1 (hsv-1) r mutants, which were defective in fusion function, were isolated from fusion inducing strains g7471 and g51, and classified into several groups by complementation (yamamoto and kabuta, 1977; yamamoto et al. 1989). marker rescue experiments of intact dna from r mutants with ecori fragments of g7471 dna revealed that the r mutations belonging to the groups a, b, e, f, g, and h were located in ecori-g, -c, -d, -h, -b, and -d fragments, respectively. the mutation ... | 1993 | 8007624 |
| epidemiology of genital herpes infections. | much has been learned in the last decade about the epidemiology of genital herpes infections, including new information about seroprevalence and the risk of transmission of genital herpes to sex partners and at delivery. unfortunately, the type-specific serologic assays now routinely used in these studies are not widely available, and commercially available assays that claim to be type-specific are not. thus, most clinicians still do not have access to reliable type-specific assays. in cross-sec ... | 1993 | 8106731 |
| effect of heparin on hemagglutinin of herpes simplex virus type 1. | heparin inhibited the hemagglutinin activity of herpes simplex virus (hsv) type 1. the minimal inhibitory concentration of heparin required to inhibit 8 hemagglutination (ha) u of hsv ranged from 0.005 to 0.01 u/ml. mouse erythrocytes failed to combine with the ha inhibitory factor of heparin. on the other hand, mouse erythrocytes treated with heparinase had greatly reduced agglutinability by hsv. virus-heparin complex formation was observed by sedimenting heparin with the virus particles. | 1993 | 8133804 |
| localized gene transfer into organotypic hippocampal slice cultures and acute hippocampal slices. | viral vectors derived from herpes simplex virus, type-1 (hsv), can transfer and express genes into fully differentiated, post-mitotic neurons. these vectors also transduce cells effectively in organotypic hippocampal slice cultures. nanoliter quantities of a virus stock of hsvlac, an hsv vector that directs expression of e. coli beta-galactosidase (beta-gal), were microapplied into stratum pyramidale or stratum granulosum of slice cultures. twenty-four hours later, a cluster of transduced cells ... | 1993 | 8152244 |
| diagnostic impression cytology for herpes simplex keratitis. | herpes simplex virus (hsv) antigens in corneal or conjunctival epithelial lesions were detected by impression cytology. specimens were obtained using a nitrocellulose membrane and were stained by the peroxidase-antiperoxidase method. hsv antigens were demonstrated in 30 of 32 patients with herpes simplex keratitis or conjunctivitis. impression specimens of dendritic or stellate keratitis lesions exhibited precise replicas of corneal lesions with numerous antigen-positive cells. hsv antigens were ... | 1993 | 8145397 |
| carriage, transfer and interaction of oral viruses and bacteria. | carriage of antigens and infectious herpes simplex virus type 1 (hsv-1) and human adenovirus type 1 (ad-1) by salivary leukocytes was compared with the antibacterial activity of oral polymorphonuclear leukocytes (pmnl) and with the spectrum of oral bacterial and fungal flora. risk of iatrogenic infections by microbes was assessed by detecting these viruses and microbes after disinfecting dental instruments. the results indicate carriage of antigens and infectious viruses in each age group betwee ... | 1993 | 8191866 |
| a cosmid-based system for constructing mutants of herpes simplex virus type 1. | cosmids containing large fragments of herpes simplex virus type 1 dna were prepared using a vector that allows intact inserts to be excised using the restriction endonuclease paci. two independent sets (a and b) of five cosmids were identified whose inserts overlap and represent the entire viral genome, and set c was obtained by replacing two cosmids in set b. each set gave rise to viral plaques when digested with paci and transfected into cells in culture. two cosmids common to sets b and c ost ... | 1993 | 8212547 |
| dna sequence of a simian varicella virus gene that encodes a homologue of varicella zoster virus ie62 and herpes simplex virus icp4. | clinical and biological studies indicate that simian varicella virus (svv) infection of primates is the counterpart of human varicella zoster virus (vzv) infection. we have identified an svv open reading frame (orf) that is homologous to the vzv protein ie62 and herpes simplex virus (hsv) icp4. like the genes encoding the vzv and hsv proteins, the svv gene is located in the repeat region of the virus genome. its expression in svv-infected cells yields a 4.2-kb transcript. dna sequencing of the s ... | 1993 | 8212583 |
| herpes simplex type 1 encephalitis in acquired immunodeficiency syndrome. | a severe acute necrotizing herpes simplex virus type 1 encephalitis, apparently mediated by macrophages or cytokines or both, was seen in a patient with acquired immunodeficiency syndrome. the necrosis had a distribution similar to that seen in immunocompetent subjects. viral inclusions were abundant and meningeal reaction was minimal, as previously described in one anergic patient. | 1993 | 8215251 |
| in vitro activity of a combretum micranthum extract against herpes simplex virus types 1 and 2. | the authors demonstrate in vitro antiviral activity of a methanolic extract of combretum micranthum leaves against hsv-1 and hsv-2. this activity is present only in the extract dissolved 7 days before the assay, but not in the freshly prepared extract, thus indicating the presence of inactive precursors which undergo spontaneous transformations into active compounds. the alkaline autooxidation of the methanolic extract promotes this rapid transformation. the precursors have been identified as co ... | 1993 | 8215303 |
| identification of important residues within the putative nucleoside binding site of hsv-1 thymidine kinase by random sequence selection: analysis of selected mutants in vitro. | random sequence mutagenesis in conjunction with genetic complementation was used to map the function of amino acid residues within the putative nucleoside binding site of the herpes simplex virus type 1 (hsv-1) thymidine kinase (tk). six codons of the putative nucleoside binding site of the hsv-1 tk were substituted by a duplex of extended oligonucleotides containing 20% random sequences. approximately 260 mutants were screened for the ability to genetically complement a tk-deficient escherichia ... | 1993 | 8218229 |
| serum antibodies to herpes simplex virus types i and ii in depressed patients. | 1993 | 8218610 | |
| exacerbation of murine herpes simplex virus-mediated stromal keratitis by th2 type t cells. | corneal infection of susceptible mice with hsv-1 causes herpetic stromal keratitis (hsk), which serves as a model of human hsk. to study the properties of the t lymphocytes involved in hsk, susceptible mice were immunized with the synthetic peptide corresponding to the amino terminal of hsv-1-associated glycoprotein d (gd 5-23). a cd4+ long-term t cell line and a clone bearing v beta 8.2 tcr were derived from peptide-primed lymph node cells. these t cells recognize gd 5-23 peptide in the context ... | 1993 | 8228262 |