| meningoencephalomyelitis caused by herpes simplex virus-1 in a patient with idiopathic t cd4+ lymphocytopenia. | | 1998 | 9484406 |
| micturitional disturbance in herpetic brainstem encephalitis; contribution of the pontine micturition centre. | micturitional disturbance is rarely mentioned in human herpetic brainstem encephalitis although the pontine tegmentum, called the pontine micturition centre, seems to regulate the lower urinary tract in experimental animals. the case of a 45 year old man, who developed subacute coma and hiccup-like dysrhythmic breathing, and needed assisted ventilation is reported. examination of csf showed mononuclear pleocytosis and antibody against herpes simplex virus type 1, but the opening pressure was 90 ... | 1998 | 9489547 |
| heterogeneity of mouse spleen dendritic cells: in vivo phagocytic activity, expression of macrophage markers, and subpopulation turnover. | in the normal mouse spleen, two distinct populations of dendritic cells (dc) are present that differ in microanatomical location. the major population of marginal dc is found in the "marginal zone bridging channels" and extends into the red pulp. the interdigitating cells (idc) are localized in the t cell areas in the white pulp. the aim of the present study was to characterize these two splenic dc populations with regard to their phenotype, in vivo phagocytic function, and turnover. both margin ... | 1998 | 9498754 |
| temporal gene regulation of the channel catfish virus (ictalurid herpesvirus 1). | to identify promoter regions that impart differential temporal regulation of channel catfish virus (ccv) genes, the transcriptional kinetics of an immediate-early gene and prospective early and late genes were characterized. a cdna clone, designated ie3c, representing a third immediate-early transcript was identified. the 5' end of the ie3c transcript was mapped to nucleotides 15,368 and 131,043 in the terminal repeat regions of the ccv genome. the full length of the transcript represented by th ... | 1998 | 9499043 |
| herpes simplex virus type 2 icp47 inhibits human tap but not mouse tap. | herpes simplex virus serotype 1 (hsv-1) expresses an immediate-early protein, icp47, that effectively blocks the major histocompatibility complex class i antigen presentation pathway. hsv-1 icp47 (icp47-1) binds with high affinity to the human transporter associated with antigen presentation (tap) and blocks the binding of antigenic peptides. hsv type 2 (hsv-2) icp47 (icp47-2) has only 42% amino acid sequence identity with icp47-1. here, we compared the levels of inhibition of human and murine t ... | 1998 | 9499125 |
| the region of the herpes simplex virus type 1 lat gene involved in spontaneous reactivation does not encode a functional protein. | we previously showed that the lat function required for efficient spontaneous reactivation of herpes simplex virus type 1 (hsv-1) from neuronal latency in the rabbit maps within the first 1.5 kb of the 8.3-kb primary lat transcript. this demonstrated that lat does not function via an antisense mechanism, since the first 1.5 kb of lat does not overlap any other known hsv-1 gene. furthermore, if lat encodes a protein essential for efficient spontaneous reactivation, it must map within the function ... | 1998 | 9501054 |
| identification of the sites of interaction between the scaffold and outer shell in herpes simplex virus-1 capsids by difference electron imaging. | formation of herpes simplex virus-1 capsids requires the presence of intact scaffolding proteins. the c terminus of the abundant scaffolding protein associates with the major capsid shell protein vp5 through hydrophobic interactions. after cleavage by the viral encoded protease, which removes their c-terminal 25 aa, the scaffolding proteins are released from the capsid. we have used electron cryomicroscopy and computer image processing to determine, to 13 a, the three-dimensional structures of c ... | 1998 | 9501166 |
| successful treatment of an acyclovir- and foscarnet-resistant herpes simplex virus type 1 lesion with intravenous cidofovir. | | 1998 | 9502489 |
| herpes simplex virus-1 and varicella virus infections in familial dysautonomia patients. | familial dystautonomia (fd) patients are deficient in type c fibers, suggesting that there may be a different pattern of infection and clinical presentation when infected by herpes simplex virus type 1 (hsv-1) or varicella-zoster virus (vzv). these viruses infect and are reactivated in the periphery of the body through type c sensory nerve fibers. hsv-1 infects epithelial cells, penetrates into type c fibers, and migrates to the ganglia to generate latent infection. in reactivation, the viral dn ... | 1998 | 9515762 |
| herpes simplex virus gd and virions accumulate in endosomes by mannose 6-phosphate-dependent and -independent mechanisms. | herpes simplex virus (hsv) glycoprotein d (gd) is modified with mannose 6-phosphate (m6p) and binds to m6p receptors (mprs). mprs are involved in the well-characterized pathway by which lysosomal enzymes are directed to lysosomes via a network of endosomal membranes. based on the impaired ability of hsv to form plaques under conditions in which glycoproteins could not interact with mprs, we proposed that mprs may function during hsv egress or cell-to-cell spread (c. r. brunetti, r. l. burke, b. ... | 1998 | 9525660 |
| structure-function analysis of the herpes simplex virus type 1 ul12 gene: correlation of deoxyribonuclease activity in vitro with replication function. | although the product of the ul12 gene of herpes simplex virus type 1 (hsv-1) has been shown to possess both exonuclease and endonuclease activities in vitro, and deletion of most of the gene within the viral genome results in inefficient production and maturation of infectious virions, the function of the deoxyribonuclease (dnase) activity per se in virus replication remains unclear. in order to correlate the in vitro and in vivo activities of the protein encoded by ul12, mutant proteins were te ... | 1998 | 9527934 |
| toxicity and neuronal infection of a hsv-1 icp34.5 mutant in nude mice. | hsv-1 mutants in the rl-1 gene encoding the icp34.5 protein have been demonstrated to have diminished neurovirulence in brain yet replicate as efficiently as parental virus in transformed tissue culture cells. thus they have been proposed as candidates viruses for human brain tumor therapies. evaluation of their replicative properties and pathogenesis within the nervous system has been limited. as most patients undergoing therapies for brain tumors are likely to be immunocompromised, it will be ... | 1998 | 9531017 |
| axonal transport of herpes simplex virus-1 in an in vitro model based on the isolated sciatic nerve of the frog rana ridibunda. | an in vitro model for the study of the axonal transport of herpes simplex virus-1 (hsv-1) in the nerve fibres of the sciatic nerve of the frog rana ridibunda, has been developed. the nerve was placed along a three-chambered bath consisting of three isolated chambers arranged in series: the stimulating, perfusion and recording chambers. the hsv-1 inoculum was placed in the stimulating chamber, where the proximal part of the isolated sciatic nerve was immersed. hsv-1 was detected after 24-36 h in ... | 1998 | 9531462 |
| the management of herpes simplex virus infection in pregnancy. | | 1998 | 9532983 |
| in vivo production of cytokines and beta (c-c) chemokines in human recurrent herpes simplex lesions--do herpes simplex virus-infected keratinocytes contribute to their production? | recurrent human herpes simplex lesions are infiltrated by macrophages and cd4 and cd8 lymphocytes, which secrete cytokines and chemokines. vesicle fluid was examined by elisa for the presence of cytokines and beta (c-c) chemokines. on the first day of the lesion, high concentrations of interleukin (il)-1beta, and il-6, moderate concentrations of il-1alpha and il-10, and low concentrations of il-12 and beta chemokines were found; levels of macrophage inflammatory protein (mip)-1beta were signific ... | 1998 | 9534953 |
| genital herpes: epidemiology and pathophysiology. update and new perspectives. | genital herpes simplex virus (hsv) is a sexually transmitted disease (std) which affects millions of people worldwide and is mainly due to hsv type 2. seroprevalence rates as high as 60-90% have been reported in developing countries. in developed countries, 20% of the general population is hsv2 seropositive. recent epidemiological surveys employing type-specific antibody assays show that the prevalence of hsv-2 infections is rising at an alarming rate. also, the epidemiology is changing with an ... | 1997 | 9538399 |
| intractable seizure disorder associated with chronic herpes infection. hsv1 detection in tissue by the polymerase chain reaction. | we describe the pathological findings and report the detection of herpes simplex virus 1 (hsv1) in the brain in three patients who presented with intractable seizures. all three patients had a previous history of hsv1 encephalitis and went on to develop a medically refractory seizure disorder necessitating surgical intervention. hsv1 encephalitis was clinically diagnosed and treated at 6 months, 3 years, and 7 months and surgical resection was done at 8.5 years, 6 years, and 3 years, in cases 1, ... | 1998 | 9548334 |
| design, synthesis, and antiviral evaluations of 1-(substituted benzyl)-2-substituted-5,6-dichlorobenzimidazoles as nonnucleoside analogues of 2,5,6-trichloro-1-(beta-d-ribofuranosyl)benzimidazole. | we have recently reported that certain ribosylated polyhalogenated benzimidazoles are potent and selective inhibitors of hcmv replication at noncytotoxic concentrations. to extend the structure-activity relationship beyond these first-generation compounds, we alkylated 5,6-dichloro-2-substituted-benzimidazoles with either a series of substituted benzyl halides or (2-bromoethyl)benzene to obtain five series of nonnucleoside analogues. evaluation of these compounds for activity against herpes viru ... | 1998 | 9548815 |
| immunization with hsv-1 antigen rapidly protects against hsv-1-induced encephalitis and is ifn-gamma independent. | herpes simplex virus type 1 (hsv-1) infection of mice frequently culminates in fatal encephalitis. intraperitoneal administration of heat-inactivated hsv-1 0-5 days before infection (active immunization) protected mice from encephalitis. in addition, active immunization 2-5 days before ocular infection with hsv-1 reduced the frequency of establishment of latent hsv-1 infection in the trigeminal ganglion (tg). however, intraperitoneal administration of heat-inactivated hsv-1 did not induce interf ... | 1998 | 9555976 |
| transport of viruses through fetal membranes: an in vitro model of perinatal transmission. | a model system for perinatal transmission of viral infections was developed and transport of infectious virus particles through fetal membranes was investigated. viruses of different families known to cause serious intrauterine infections were selected, including relevant and model viruses: the dna-viruses hsv-1 and -2 as well as the animal herpes viruses bhv-1 and shv-1, the rna-virus bvdv as a model for hepatitis c virus, hiv-1 and -2, and ppv as a model for parvovirus b19. migration of infect ... | 1998 | 9557298 |
| infrequent detection of cytomegalovirus and epstein-barr virus dna in synovial membrane of patients with rheumatoid arthritis. | to study the role of the cytomegalovirus (cmv), epstein-barr virus (ebv), herpes simplex virus types 1 and 2 (hsv-1 and 2), varicella zoster virus (vzv), and human herpes virus 6 (hhv-6) in the etiology of rheumatoid arthritis (ra). | 1998 | 9558160 |
| site-directed mutagenesis probing the catalytic role of arginines 165 and 166 of human cytomegalovirus protease. | human cytomegalovirus (cmv) is a member of the herpesviridae family of viruses that also includes herpes simplex viruses (hsv-1 and hsv-2), varicella-zoster virus (vzv), human herpes virus-6, 7, and 8 (hhv-6, hhv-7, and hhv-8), and epstein-barr virus (ebv). each member of this family encodes a serine protease that is a potential target for antiviral therapeutic intervention. we recently reported the crystal structure of cmv proteases [qiu, x., culp, j. s., dilella, a. g., hellmig, b., hoog, s. s ... | 1998 | 9558326 |
| glycoprotein c-deficient mutants of two strains of herpes simplex virus type 1 exhibit unaltered adsorption characteristics on polarized or non-polarized cells. | mutants of herpes simplex virus type 1 (hsv-1) strain sc16, lacking each of the dispensable glycoproteins c, g, e, i or j, were examined for their ability to infect the apical or basolateral surfaces of polarized human epithelial cells. none of the mutants was significantly different from the wild-type parent when assayed on either surface. since a previous report had demonstrated that glycoprotein c (gc) was necessary for the infection of apical surfaces of polarized epithelium, a second gc-neg ... | 1998 | 9568976 |
| human cytomegalovirus glycoprotein h/glycoprotein l complex modulates fusion-from-without. | glycoprotein h/glycoprotein l (gh/gl) complexes of herpesviruses are required for fusion of infecting virions with host cell membranes. in human cytomegalovirus (hcmv), neutralizing monoclonal antibodies (mab) specific for gh inhibit the transfer of a fluorescent probe to the host cell from labelled virus particles. in similar fashion, in the present study, neutralizing gh-specific mab inhibited hcmv-induced fusion-from-without in monolayers of both human embryonic fibroblasts and continuous ast ... | 1998 | 9568982 |
| evaluation of the galalpha1-3gal epitope as a host modification factor eliciting natural humoral immunity to enveloped viruses. | human sera contain high levels of natural antibody (ab) to galalpha1-3gal, a terminal glycosidic structure expressed on the surface of cells of mammals other than old world primates. incorporation of this determinant onto retroviral membranes by passage of viruses in cells encoding alpha-1-3-galactosyltransferase (gt) renders retroviruses sensitive to lysis by natural ab and complement in normal human serum (nhs). plasma membrane-budding viruses representing four additional virus groups were exa ... | 1998 | 9573228 |
| trends in seroprevalence of human papillomavirus type 16 among pregnant women in stockholm, sweden, during 1969-1989. | to assess long-term trends in the prevalence of oncogenic human papillomavirus (hpv) infection, we performed a cross-sectional serosurvey of the seroprevalence of the major oncogenic hpv type, hpv16, among 3,512 pregnant women undergoing population-based serological screening at the first trimester of pregnancy in the same catchment area in stockholm, sweden, during 1969, 1983 or 1989. the overall hpv16 seroprevalence rates were 16% in 1969, 22% in 1983 and 21% in 1989. seroprevalence was signif ... | 1998 | 9579570 |
| [application of the polymerase chain reaction to an experimental model of infection by herpes simplex virus type 1]. | herpes simplex type 1 (hsv-1) has been implicated as a cause of facial paralysis. we developed an experimental model in rabbits in which we injected hsv-1 into the tongue. the animals were killed after one and three weeks. the geniculate and trigeminal ganglia and medulla were extracted and the samples were processed. the polymerase chain reaction (pcr), nested-pcr and cultures were carried out in order to detect the viral genome in the samples. the viral genome was found in all but two rabbits. ... | 1998 | 9580465 |
| differential ganciclovir-mediated cytotoxicity and bystander killing in human colon carcinoma cell lines expressing herpes simplex virus thymidine kinase. | the two human colon carcinoma cell lines ht-29 and sw620, which stably express herpes simplex virus thymidine kinase (hsv-tk), are sensitized to the cytotoxic effects of the antiviral drug ganciclovir (gcv). compared with ht-29 cells, sw620 cells were more sensitive to lower gcv concentrations (<1 microm), accumulated gcv triphosphate more rapidly, and incorporated higher levels of gcv into dna. following a 24-hr exposure to 10 microm gcv, bystander killing was as much as sixfold greater in sw62 ... | 1998 | 9581903 |
| double suicide gene (cytosine deaminase and herpes simplex virus thymidine kinase) but not single gene transfer allows reliable elimination of tumor cells in vivo. | suicide genes such as cytosine deaminase (cd) and herpes simplex virus thymidine kinase (tk) encode products that convert nontoxic substances (prodrugs) into toxic metabolites. suicide gene transfer is currently being used in cancer therapy or can be used as a safety modality. to analyze the reliability of suicide genes as a safety modality for a vaccination study with viable cytokine/b7 gene-modified tumor cells, the individual and combined efficacy of the two suicide genes was compared for in ... | 1998 | 9581908 |
| cochinolide, a new gamma-alkylidene bicyclic butenolide with antiviral activity, and its beta-glucopyranoside from homalium cochinchinensis. | a new gamma-alkylidene bicyclic butenolide designated as cochinolide (1) and its beta-glucopyranoside (3) were isolated from the root bark of homalium cochinchinensis (flacoutiaceae). their structures, except absolute stereochemistries, were determined by spectroscopic means. cochinolide (1) showed moderate antiviral activities against hsv-1 and -2. | 1998 | 9584406 |
| [impact of focal allergic test with herpetic vaccine in diagnosis of ophthalmic herpes]. | clinical and immunological studies were carried out in 697 patients (913 eyes) for a more accurate diagnosis of herpetic involvement of the posterior segment of the eye by the focal allergic test (fat) based on the delayed type hypersensitivity phenomenon. fat consists in detection of the focal reaction in the eye, regularly developing after 2-3 intracutaneous injections of herpetic vaccine to types i and ii herpes simplex virus in a dose of 0.2 ml every other day. the reaction is assessed by cl ... | 1998 | 9584632 |
| synthesis of 2'-deoxy-2'-fluoro-4'-thioarabinonucleosides using the pummerer rearrangement and their biological evaluation. | searching for more effective antineoplastic and antiviral agents, we have prepared various 2'-deoxy-2'-fluoro-4'-thioarabinonucleosides. the glycosylation reaction of persilylated pyrimidine bases with a 4-thiosugar derivative was performed using sncl4 as a catalyst. the same reaction between purines and the 4-thiosugar, catalyzed by tmsotf, gave the corresponding purine 4'-thionucleosides. pyrimidine derivatives (e.g., 5-ethyluracil, 5-iodouracil, and 5-iodocytosine) showed potent anti-hsv-1 ac ... | 1997 | 9585990 |
| herpes simplex virus-induced recurrent erythema multiforme. | | 1995 | 9594805 |
| [preclinical studies on thymidine kinase gene (tk) and gancyclovir system for treatment of malignant astrocytoma]. | to study preclinically tk gene mediated gcv system for treatment of human malignant astrocytoma. | 1997 | 9596975 |
| effect of viral infections on the ability of human endothelium for interferon, tumor necrosis factor and interleukin 6 production. | we have reported that cultured human umbilical cord vein endothelial cells (huvec) differ from endothelium present on vein surface of organ culture (oc) in production of cytokines and susceptibility to viral infections. in this paper we present the effect of viral infections on interferon (ifn), tumor necrosis factor (tnf), and interleukin 6 (il-6) production in two culture systems: huvec and oc. infection of 24-48 h huvec with herpes simplex type 1 (hsv-1) and vesicular stomatitis virus (vsv) r ... | 1997 | 9597094 |
| evaluation of biological activities of triterpenoid saponins from maesa lanceolata. | from a bioassay-guided fractionation procedure using herpes simplex virus type 1 as the target model, a virucidal saponin mixture (maesasaponin mixture b) was isolated from the meoh extract of leaves of maesa lanceolata. the maesasaponin mixture b consisted of six homologous oleanane-type triterpenoid saponins 1-6, identified by 1h, 13c, and 2d nmr spectroscopy and mass spectrometry. the maesasaponin mixture b (1-6) showed several biological activities expected for saponins. it exhibited a moder ... | 1998 | 9599254 |
| transfection of cos cells with human cystatin cdna and its effect on hsv-1 replication. | | 1998 | 9599312 |
| an indolent penile herpetic ulcer in a patient with previously undiagnosed human immunodeficiency virus infection. | we report a case of an atypical penile herpetic ulcer in a man with previously undiagnosed human immunodeficiency virus (hiv) infection. swabs of the lesion were negative for herpes simplex virus (hsv) by culture and electron microscopy. however, histopathology of biopsy material showed characteristic herpetic multinucleate giant cells. immunochemistry using polyclonal antibodies was positive for hsv1 and hsv2. the lesion completely resolved after treatment with aciclovir and the patient subsequ ... | 1998 | 9602886 |
| herpes simplex virus hepatitis in macrophage-depleted mice: the role of massive, apoptotic cell death in pathogenesis. | infection with herpes simplex virus or hepatitis viruses can lead to fulminant hepatitis, but there is controversy about the underlying conditions needed for such disease. to investigate how the impairment of host defences might be involved, macrophages were depleted by administration of silica to mice before intravenous injection with herpes simplex virus type 1 (hsv-1). such mice died rapidly and their livers were yellowish and shrunken (acute yellow atrophy), and occasionally grossly haemorrh ... | 1998 | 9603338 |
| does the latency associated transcript (lat) of herpes simplex virus (hsv) function as a ribozyme during viral reactivation? | the latency associated transcript (lat) of herpes simplex virus (hsv) appears to exist as an rna molecule only. this phenomenon is consistent with the concept of functioning at an rna level, and several lines of evidence suggest that the lat may be a ribozyme. this provides an insight into understanding the role of lat during hsv reactivation. | 1998 | 9608658 |
| activity of n-chlorotaurine against herpes simplex- and adenoviruses. | n-chlorotaurine, an essential weak oxidant produced by stimulated human leukocytes, is known to have bactericidal, fungicidal and vermicidal properties. this study for the first time demonstrates its virucidal activity. by viral suspension tests at incubation times between 5 and 60 min, virus titers of both herpes simplex virus type 1 and 2 were reduced about 1.3-2.9 log10 and 2.8-4.2 log10 by 0.1 and 1%, (5.5 and 55 mm) n-chlorotaurine, respectively. virus titer reduction of adenovirus type 5 b ... | 1998 | 9614001 |
| entry of alphaherpesviruses mediated by poliovirus receptor-related protein 1 and poliovirus receptor. | a human member of the immunoglobulin superfamily was shown to mediate entry of several alphaherpesviruses, including herpes simplex viruses (hsv) 1 and 2, porcine pseudorabies virus (prv), and bovine herpesvirus 1 (bhv-1). this membrane glycoprotein is poliovirus receptor-related protein 1 (prr1), designated here as hvec. incubation of hsv-1 with a secreted form of hvec inhibited subsequent infection of a variety of cell lines, suggesting that hvec interacts directly with the virus. poliovirus r ... | 1998 | 9616127 |
| social stress and the reactivation of latent herpes simplex virus type 1. | psychological stress is thought to contribute to reactivation of latent herpes simplex virus (hsv). although several animal models have been developed in an effort to reproduce different pathogenic aspects of hsv keratitis or labialis, until now, no good animal model existed in which application of a psychological laboratory stressor results in reliable reactivation of the virus. reported herein, disruption of the social hierarchy within colonies of mice increased aggression among cohorts, activ ... | 1998 | 9618568 |
| a therapeutic vaccine that reduces recurrent herpes simplex virus type 1 corneal disease. | to investigate the therapeutic efficacy of periocular vaccination with herpes simplex virus (hsv) recombinant glycoprotein d from hsv-1 (gd1) or hsv-2 (gd2) in decreasing hsv-induced recurrent dendritic keratitis and hsv-induced recurrent ocular shedding in rabbits latently infected with hsv-1. | 1998 | 9620075 |
| characterization of a nerve growth factor-inducible cellular activity that enhances herpes simplex virus type 1 gene expression and replication of an icp0 null mutant in cells of neural lineage. | herpes simplex virus type 1 (hsv-1) icp0 is required for efficient viral gene expression during lytic infection, especially at low multiplicities. a series of cellular activities that can substitute for icp0 has been identified, suggesting that when the activity of icp0 is limiting, these activities can substitute for icp0 to activate viral gene expression. the cellular activities may be especially important during reactivation of hsv from neuronal latency when viral gene expression is initiated ... | 1998 | 9620991 |
| [the development of a method for viral inactivation using thiophosphamide]. | using three-functional alkylating compound thiophosphamide complete inactivation of the influenza and herpesviruses of type 1 and 2 was achieved. the method provides full inactivation of dna- and rna-containing viruses and preservation of their immunogenic properties. | 1998 | 9621691 |
| diagnosis of herpetic keratoconjunctivitis by nested polymerase chain reaction in human tear film. | a study was performed to evaluate nested pcr (npcr) versus viral cultures as method and tear film versus corneal scrapings as specimen in the diagnosis of viral keratoconjunctivitis. tear film specimens were taken from both eyes and corneal scrapings from the affected eye only in 17 patients with suspected viral keratoconjunctivitis. in 15 of the 17 patients the viral agent of the infection could be detected: 11 patients had herpes simplex virus type 1, two varicella-zoster virus, one both herpe ... | 1998 | 9629979 |
| application of automated thermal disinfection instead of sterilisation procedures for treatment of rotating dental instruments: efficacy against viruses? | in dentistry it is of primary importance to take into consideration microbial transfer due to the nature of the construction of rotating dental instruments. this aspect was the starting point for our research with the question whether or not sterilisation is fundamentally necessary for slow and high speed hand pieces to make them "safe" out of a virological point of view, or whether a thermal disinfection could also possibly be adequate for this purpose. in this context, we tested the efficiency ... | 1997 | 9636990 |
| isolation of a herpes simplex virus type 1-like agent from the brain of a mountain agile gibbon (hylobates agilis agilis) with encephalitis. | a captive-born 2-yr-old male mountain agile gibbon (hylobates agilis agilis) that died of encephalitis harbored a herpes simplex virus type 1 (hsv1)-like agent in the brain. a complete necropsy revealed intensive meningeal congestion with nonsuppurative encephalitis. the virus was recovered from frozen brain tissue in vero cells. the isolate was very similar but not identical to human hsv1. both western blot assay and virus neutralization tests were done with sera from 15 gibbons. antibodies aga ... | 1998 | 9638628 |
| hsv-1 in brain and risk of alzheimer's disease. | | 1998 | 9643802 |
| characterization of the ul16 gene product of herpes simplex virus type 2. | we have raised rabbit polyclonal antisera against a his-tagged herpes simplex virus type 1 (hsv-1) ul16 fusion protein, one of which very specifically reacted with 40 kda and 41 kda proteins in the lysates of hsv-1 and hsv-2-infected cells, respectively. since its reactivity to the 41 kda protein was clearly eliminated by pre-adsorption with e. coli lysates expressing the ul16 fusion protein, the antiserum was used to characterize the ul16 products of hsv-2. the hsv-2 ul16 protein was produced a ... | 1998 | 9645194 |
| rapid detection, culture-amplification and typing of herpes simplex viruses by enzyme immunoassay in clinical samples. | the laboratory diagnosis of herpes simplex infection may require rapid (direct) tests, as well as cell cultures, for detection of the virus in clinical samples. the quantity of virus present in clinical samples is variable and this may depend on the period from onset of rash. in addition, not all patients may show obvious symptoms with this infection. the successful culture of herpes simplex virus requires prompt transportation after collection of the specimen as the virus is easily inactivated. ... | 1998 | 9646003 |
| molecular methods for the diagnosis of genital ulcer disease in a sexually transmitted disease clinic population in northern thailand: predominance of herpes simplex virus infection. | a multiplex polymerase chain reaction (m-pcr) assay that simultaneously detects the three major causes of genital ulcer disease (gud), haemophilus ducreyi, treponema pallidum, and herpes simplex virus, was used to evaluate swab specimens for 38 sequential patients with gud at a thai sexually transmitted disease clinic. subjects received clinical diagnoses and syndromic treatment. swab specimens for h. ducreyi cultures and m-pcr were obtained. no h. ducreyi cultures were positive. of 38 m-pcr spe ... | 1998 | 9652447 |
| mutations in the exo iii motif of the herpes simplex virus dna polymerase gene can confer altered drug sensitivities. | two herpes simplex virus mutants containing mutated residues within the conserved exo iii motif of the polymerase gene were previously shown to be defective in 3'-5' exonuclease activity and exhibited extremely high mutation frequencies. in this study, we have shown that these mutants also exhibited higher resistance to phosphonoacetic acid and sensitivity to aphidicolin and all nucleoside analogs tested, including acyclovir and gangciclovir, compared to wild-type virus. marker transfer experime ... | 1998 | 9657948 |
| the acidic domain of pul37x1 and gpul37 plays a key role in transactivation of hcmv dna replication gene promoter constructions. | transient complementation of human cytomegalovirus (hcmv) orilyt dna replication in permissive human diploid cells expressing replication genes under native promoters requires its ul36-38 gene products. two of the immediate early (ie) proteins encoded by this locus, pul37x1 and, to a lesser extent, gpul37, activated expression of hcmv early gene promoter constructions. the other ie protein encoded by the ul36-38 locus, pul36, and the early product, pul38, did not transactivate the hcmv early pro ... | 1998 | 9657958 |
| human thymidine kinase can functionally replace herpes simplex virus type 1 thymidine kinase for viral replication in mouse sensory ganglia and reactivation from latency upon explant. | herpes simplex virus type 1 thymidine kinase exhibits a strikingly broad substrate specificity. it is capable of phosphorylating deoxythymidine and deoxyuridine as does human thymidine kinase, deoxycytidine as does human deoxycytidine kinase, the cytosolic kinase whose amino acid sequence it most closely resembles, and thymidylate as does human thymidylate kinase. following peripheral inoculation of mice, viral thymidine kinase is ordinarily required for viral replication in ganglia and for reac ... | 1998 | 9658118 |
| the probability of in vivo reactivation of herpes simplex virus type 1 increases with the number of latently infected neurons in the ganglia. | the purpose of this study was to define the relationship between herpes simplex virus (hsv) latency and in vivo ganglionic reactivation. groups of mice with numbers of latently infected neurons ranging from 1.9 to 24% were generated by varying the input titer of wild-type hsv type 1 strain 17syn+. reactivation of the virus in mice from each group was induced by hyperthermic stress. the number of animals that exhibited virus reactivation was positively correlated with the number of latently infec ... | 1998 | 9658140 |
| acyclic nucleoside phosphonates in the chemotherapy of dna virus and retrovirus infections. | the acyclic nucleoside phosphonates [hpmpc (cidofovir), pmea (adefovir) and pmpa] have proved to the effective in vitro (cell culture systems) and in vivo (animal models, clinical studies) against a wide variety of dna virus and retrovirus infections: i.e., hpmpc against herpesvirus (herpes simplex virus type 1 and 2, varicella-zoster virus, cytomegalovirus, epstein-barr virus, human herpesvirus types 6, 7, and 18), polyoma-, papilloma-, adeno-, and poxvirus (vaccinia virus, molluscum contagiosu ... | 1997 | 9675635 |
| [diagnosis of herpetic uveitis and keratouveitis]. | in epithelial viral keratitis as in viral retinitis, the diagnosis is made on the basis of typical clinical findings. a laboratory confirmation is achieved in over 80% using routine laboratory methods. in contrast, it is almost impossible to confirm the diagnosis of stromal herpetic keratitis in vivo using the currently available laboratory methods. nothing is known about the situation in cases of viral anterior uveitis. | 1998 | 9677579 |
| viral encephalitis: a challenging diagnosis in an icu. | | 1998 | 9677939 |
| distinct domains in herpes simplex virus type 1 us11 protein mediate post-transcriptional transactivation of human t-lymphotropic virus type i envelope glycoprotein gene expression and specific binding to the rex responsive element. | herpes simplex virus type 1 (hsv- 1) us11 protein is an rna-binding protein which is able to mediate post-transcriptional transactivation of human t-lymphotropic virus type i (htlv-i) envelope glycoprotein gene expression by interacting with the rex responsive element (xre) located at the 3' end of the env mrna. in view of this functional activity, and because us11 protein is capable of substituting for htlv-i rex protein, it was hypothesized that us11 protein should exhibit at least two functio ... | 1998 | 9680120 |
| 3,6-diamino-10-methylacridan: uncharged precursor of acriflavine and its unique antimicrobial activity. | | 1998 | 9685231 |
| effect of the extract of annona muricata and petunia nyctaginiflora on herpes simplex virus. | annona muricata (annonaceae) and petunia nyctaginiflora (solanaceae) were screened for their activity against herpes simplex virus-1 (hsv-1) and clinical isolate (obtained from the human keratitis lesion). we have looked at the ability of extract(s) to inhibit the cytopathic effect of hsv-1 on vero cells as indicative of anti-hsv-1 potential. the minimum inhibitory concentration of ethanolic extract of a. muricata and aqueous extract of p. nyctaginiflora was found to be 1 mg/ml. | 1998 | 9687085 |
| functional region iv of glycoprotein d from herpes simplex virus modulates glycoprotein binding to the herpesvirus entry mediator. | glycoprotein d (gd) of herpes simplex virus (hsv) is essential for virus entry and has four functional regions (i to iv) important for this process. we previously showed that a truncated form of a functional region iv variant, gd1(delta290-299t), had an enhanced ability to block virus entry and to bind to the herpesvirus entry mediator (hveat; formerly hvemt), a cellular receptor for hsv. to explore this phenotype further, we examined other forms of gd, especially ones with mutations in region i ... | 1998 | 9696802 |
| phosphorylation of structural components promotes dissociation of the herpes simplex virus type 1 tegument. | the role of phosphorylation in the dissociation of structural components of the herpes simplex virus type 1 (hsv-1) tegument was investigated, using an in vitro assay. addition of physiological concentrations of atp and magnesium to wild-type virions in the presence of detergent promoted the release of vp13/14 and vp22. vp1/2 and the ul13 protein kinase were not significantly solubilized. however, using a virus with an inactivated ul13 protein, we found that the release of vp22 was severely impa ... | 1998 | 9696804 |
| an enhanced packaging system for helper-dependent herpes simplex virus vectors. | helper-dependent herpes simplex virus (hsv) vectors (amplicons) show considerable promise to provide for long-term transduced-gene expression in most cell types. the current packaging system of choice for these vectors involves cotransfection with a set of five overlapping cosmids that encode the full hsv type 1 (hsv-1) helper virus genome from which the packaging (pac) elements have been deleted. although both the helper virus and the hsv amplicon can replicate, only the latter is packaged into ... | 1998 | 9696807 |
| phenotypic and genotypic characterization of acyclovir-resistant herpes simplex viruses from immunocompromised patients. | phenotypic and genotypic analyses were done on 30 acyclovir-resistant and 5 acyclovir-susceptible herpes simplex virus (hsv) isolates (22 hsv type 1 and 13 hsv type 2) recovered from 24 subjects. all isolates were susceptible to foscarnet. the phenotypes of the acyclovir-resistant hsv isolates were as follows: 17 were thymidine kinase (tk) deficient, 12 had decreased tk activity (produced low amounts of viral tk) or tk with altered substrate specificity, and 1 was undetermined. sequencing analys ... | 1998 | 9697707 |
| tnf-alpha expression and herpes simplex infection in human monocytes treated with growth hormone, prolactin and insulin. | we evaluated the in vitro effect of growth hormone (gh), prolactin (prl) and insulin treatment of human monocytes on herpes simplex virus type 1 (hsv-1) infection. gh and prl increased cell susceptibility to infection which was related to a slight tnf-alpha expression and release. insulin had no significant effect. cells activated with lipopolysaccharide (lps) and then treated with prl showed a lower susceptibility to hsv infection related to a significant increase in tnf-alpha expression and re ... | 1998 | 9699200 |
| antiviral activity of fa-11h on the infectivity and replication of herpes simplex virus type 1 in cultured cells. | the highest concentration lacking cytotoxicity (h.c.l.c.) of the acid fa-11h, related to ester fa-11 (ethyl 4-trifluoromethyl-2-methylthio-5-pyrimidinecarboxylate), was determined on vero cultured cells. at this concentration (300 micrograms/ml) fa-11h produced an 81.4% inhibition of herpes simplex virus type 1 infectivity with respect to the plaque formation detected in control infected cultures. this inhibitory activity was substantiated by studies on progeny production. fa-11h revealed a high ... | 1998 | 9699209 |
| an antivirally active sulfated polysaccharide from sargassum horneri (turner) c. agardh. | a sulfated polysaccharide was isolated from the hot water extract of a brown alga, sargassum horneri (turner) c. agardh. fucose was detected as the main component sugar of this polysaccharide. this compound showed potent antiviral activity against herpes simplex virus type 1, human cytomegalovirus and human immunodeficiency virus type 1. time-of-addition experiments suggested that it inhibited not only the initial stages of viral infection, such as attachment to and penetration into host cells, ... | 1998 | 9703258 |
| herpes simplex type 1 induction of persistent nf-kappa b nuclear translocation increases the efficiency of virus replication. | the latent form of the dimeric transcription factor nf-kappa b is sequestered in the cytoplasm by proteins containing ankyrin repeats, such as 1 kappa b alpha and beta, or by the p105 precursor form of the nf-kappa b p50 subunit. tumor necrosis factor alpha or virus infection can cause targeted destruction of 1 kappa b and nuclear translocation of nf-kappa b. following translocation, nf-kappa b mediates immune, inflammatory, or anti-apoptotic responses. here we present evidence that beginning at ... | 1998 | 9705914 |
| rd6-2198, a novel betain-type fluoroalkylated oligomer, inhibits the replications of human immunodeficiency virus type 1 and other enveloped viruses. | we have examined a novel betain-type fluoroalkylated oligomer, rd6-2198, for its inhibitory effects on the replication of human immunodeficiency virus type 1 (hiv-1) and other enveloped viruses, including herpes simplex virus types 1 and 2 (hsv-1 and hsv-2, respectively) and respiratory syncytial virus (rsv) in cell cultures. we have found that the compound is a potent and selective inhibitor of these viruses. rd6-2198 inhibited the replication of hiv-1iiib at a concentration of 0.85 microg/ml w ... | 1998 | 9707376 |
| exploring the active site of herpes simplex virus type-1 thymidine kinase by x-ray crystallography of complexes with aciclovir and other ligands. | antiherpes therapies are principally targeted at viral thymidine kinases and utilize nucleoside analogs, the triphosphates of which are inhibitors of viral dna polymerase or result in toxic effects when incorporated into dna. the most frequently used drug, aciclovir (zovirax), is a relatively poor substrate for thymidine kinase and high-resolution structural information on drugs and other molecules binding to the target is therefore important for the design of novel and more potent chemotherapy, ... | 1998 | 9715911 |
| tissue specific distribution of the herpes simplex virus type 1 latency-associated transcripts on polyribosomes during latent infection. | transcription of herpes simplex virus type 1 (hsv-1) during latency produces two abundant latency-associated transcripts (lats). we have recently shown, that during hsv-1 latency in mice trigeminal ganglia (tg) lats are bound to polyribosomes (j virol, 1997, 71, 2897-2904). in order to study the possible role of this binding in the latency process, we now extend the polyribosomal analysis to brainstem tissues of latently infected mice, that unlike tg do not support viral reactivation. we report ... | 1998 | 9718134 |
| synthesis and evaluation of 2-amino-9-(3-hydroxymethyl-4-alkoxycarbonyloxybut-1-yl)purines as potential prodrugs of penciclovir. | a series of 2-amino-9-(3-hydroxymethyl-4-alkoxycarbonyloxybut-1-yl)purines (4-10) and 2-amino-9-(2-(2-oxo-1,3-dioxan-5-yl)ethyl)purine (1) were synthesized as potential prodrugs of penciclovir and evaluated for their oral penciclovir bioavailability in mice and rats. treatment of 2-(2-benzyloxyethyl)propane-1,3-diol (11) with 1,1'-carbonyldiimidazole in thf followed by hydrogenolytic removal of the benzyl group of the resulting cyclic carbonate 12 gave 5-(2-hydroxyethyl)-1,3-dioxan-2-one (13). m ... | 1998 | 9719596 |
| suicide gene therapy of chemically induced mammary tumor in rat: efficacy and distant bystander effect. | we examined the efficacy of suicide gene therapy for nitrosomethylurea-induced mammary tumors in rats. individual tumors were directly injected with a retrovirus-producing cell line that releases retroviral vectors that transduce the herpes simplex virus type 1 thymidine kinase (hsv1-tk) gene. hsv1-tk specifically converts the nucleoside analogue ganciclovir (gcv) into a toxic metabolite. compared to control rats receiving saline, we observed a significant tumor regression of the injected tumors ... | 1998 | 9721854 |
| the herpes simplex virus type 1 origin binding protein. specific recognition of phosphates and methyl groups defines the interacting surface for a monomeric dna binding domain in the major groove of dna. | the ul9 gene of herpes simplex virus type 1 (hsv-1) encodes an origin binding protein (obp). it is an atp-dependent dna helicase and a sequence-specific dna-binding protein. the latter function is carried out by the c-terminal domain of obp (deltaobp). we have now performed a quantitative analysis of the interaction between deltaobp and its recognition sequence, gttcgcac, in oris. initially optimal conditions for binding were carefully determined. we observed that complexes with different electr ... | 1998 | 9733759 |
| antigenic variation of varicella zoster virus fc receptor ge: loss of a major b cell epitope in the ectodomain. | varicella zoster virus (vzv) is considered to possess a genetically stable genome; only one serotype is recognized around the world. the 125-kbp genome contains approximately 70 open reading frames. one that has received particular attention is open reading frame 68, which codes for glycoprotein ge, the predominant 623-residue viral envelope product that harbors both b and t cell epitopes. this report describes the initial characterization of a community-acquired vzv isolate that was a distingui ... | 1998 | 9740773 |
| modulation of virus-induced delayed-type hypersensitivity by plasmid dna encoding the cytokine interleukin-10. | this report evaluates the efficacy of eukaryotic expression plasmids encoding cytokines at modulating the induction and expression of cutaneous delayed-type hypersensitivity (dth) responses to virus infections. mice given a single intramuscular administration of cytokine dna were subsequently infected with either herpes simplex virus (hsv) or vaccinia virus, then tested for dth. responses in animals given interleukin-10 dna were markedly suppressed for at least 5 weeks after pretreatment. animal ... | 1998 | 9741332 |
| limited use of cationic liposomes as tools to enhance the antiherpetic activities of oligonucleotides in vero cells infected with herpes simplex virus type 1. | we used commercially available cationic liposomes, lipofectin, dotap, and transfectam, to enhance the antiherpetic activities of phosphodiester oligonucleotides (d-oligos) or phosphorothioate oligonucleotides (s-oligos) targeted against immediate-early pre-mrna4/5 of herpes simplex virus type 1 (hsv-1). with a 5-fold excess of s-oligos/d-oligos, formation of complexes with some of the s-oligos/d-oligos and the cationic liposomes could be visualized on agarose gel. a >5-fold excess of cationic li ... | 1998 | 9743464 |
| polymorphisms of thymidine kinase gene in herpes simplex virus type 1: analysis of clinical isolates from herpetic keratitis patients and laboratory strains. | drug-resistance of herpes simplex virus (hsv) is caused most frequently by mutation of the viral thymidine kinase (tk) gene. to elucidate the significance of detecting nucleotide changes of the tk gene for screening drug-resistant viruses, the frequency and variation of the genetic polymorphisms in the whole coding region of the tk gene were studied in 14 acyclovir-susceptible hsv type 1 (hsv-1) clinical isolates from 14 patients with epithelial herpetic keratitis. two reference hsv-1 laboratory ... | 1998 | 9746072 |
| nuclear redistribution of brca1 during viral infection. | the functions and the intracellular localization of the breast/ovarian susceptibility gene product, brca1, has been controversial. to arrive at a clear understanding of its localization and relative position to other nuclear structures, a new monoclonal antibody was produced and characterized by immunohistochemical techniques with other brca1 antibodies. each of the antibodies specifically detected brca1 as localized to specific nuclear domains and did so in a variety of cells and in a cell cycl ... | 1998 | 9751118 |
| the antiviral activity of the ribonucleotide reductase inhibitor bild 1351 se in combination with acyclovir against hsv type-1 in cell culture. | bild 1351 se is a selective peptidomimetic subunit association inhibitor of the herpes simplex virus (hsv) ribonucleotide reductase (rr) with potent antiviral activity both in cell culture assays and animal models of hsv disease. the ability of bild 1351 se to inhibit the replication of hsv-1 when used in combination with acyclovir (acv) for the treatment of hsv infections was investigated in baby hamster kidney cells using a 96-well enzyme-linked immunosorbent assay. the effective concentration ... | 1998 | 9754948 |
| antiviral activity of viracea against acyclovir susceptible and acyclovir resistant strains of herpes simplex virus. | viracea, a topical microbicide, is a blend of benzalkonium chloride and phytochemicals derived from echinacea purpurea and is a proprietary formula from destiny biomedix corp. viracea was tested against 40 strains of herpes simplex virus (hsv): 15 strains (five hsv-1 and ten hsv-2) were resistant to acyclovir (acv-r) and 25 strains (13 hsv-1 and 12 hsv-2) were susceptible to acv (acv-s). the median ed50 of viracea for the five acv-r strains of hsv-1 was a 1:100 dilution of the drug with a range ... | 1998 | 9754950 |
| highly variable distribution of hsv-1-specific dna in human geniculate, vestibular and spiral ganglia. | viral reactivation in temporal ganglia is the suspected cause of bell's palsy, vestibular neuritis and sudden hearing loss. since the distribution of latent herpes simplex type 1 (hsv-1) in geniculate, vestibular and spiral ganglia of individual human temporal bones could have implications for the explanation of isolated as well as combined disorders of these three cranial nerves, we examined these ganglia in 18 human temporal bones of adults by nested polymerase chain reaction. in all of the te ... | 1998 | 9756341 |
| the herpes simplex virus us11 protein effectively compensates for the gamma1(34.5) gene if present before activation of protein kinase r by precluding its phosphorylation and that of the alpha subunit of eukaryotic translation initiation factor 2. | in herpes simplex virus-infected cells, viral gamma134.5 protein blocks the shutoff of protein synthesis by activated protein kinase r (pkr) by directing the protein phosphatase 1alpha to dephosphorylate the alpha subunit of eukaryotic translation initiation factor 2 (eif-2alpha). the amino acid sequence of the gamma134.5 protein which interacts with the phosphatase has high homology to a domain of the eukaryotic protein gadd34. a class of compensatory mutants characterized by a deletion which r ... | 1998 | 9765401 |
| in vitro processing of herpes simplex virus type 1 dna replication intermediates by the viral alkaline nuclease, ul12. | herpes simplex virus type 1 (hsv-1) dna replication intermediates exist in a complex nonlinear structure that does not migrate into a pulsed-field gel. genetic evidence suggests that the product of the ul12 gene, termed alkaline nuclease, plays a role in processing replication intermediates (r. martinez, r. t. sarisky, p. c. weber, and s. k. weller, j. virol. 70:2075-2085, 1996). in this study we have tested the hypothesis that alkaline nuclease acts as a structure-specific resolvase. cruciform ... | 1998 | 9765421 |
| bell's palsy associated with herpes simplex gingivostomatitis. a case report. | bell's palsy is a sudden, isolated, peripheral facial paralysis caused by various known and sometimes unknown factors. the case of an 18-year-old man who developed bell's palsy after onset of primary herpetic gingivostomatitis is presented. although bell's palsy has already been associated with herpes simplex virus type 1, the described case is the first in the literature in which enzyme-linked immunosorbent assays for immunoglobulin g to herpes simplex virus type 1 and herpes simplex virus type ... | 1998 | 9768417 |
| x-ray analysis of a complex of escherichia coli uracil dna glycosylase (ecudg) with a proteinaceous inhibitor. the structure elucidation of a prokaryotic udg. | uracil-dna glycosylase (udg), a key highly conserved dna repair enzyme involved in uracil excision repair, was discovered in escherichia coli . the bacillus subtilis bacteriophage, pbs-1 and pbs-2, which contain dump residues in their dna, express a udg inhibitor protein, ugi which binds to udg very tightly to form a physiologically irreversible complex. the x-ray analysis of the e. coli udg ( ec udg)-ugi complex at 3.2 a resolution, leads to the first structure elucidation of a bacterial udg mo ... | 1998 | 9776748 |
| infection of the corneal endothelium in herpes simplex keratitis. | to describe viral infection of the corneal endothelium in a patient with recurrent herpes simplex virus keratitis in the corneal graft. | 1998 | 9780108 |
| [molecular biology and molecular genetics in otorhinolaryngology--virus infections in inner ears]. | | 1998 | 9786744 |
| apolipoprotein e and herpes simplex virus 1 in alzheimer's disease. | | 1998 | 9788486 |
| acute viral encephalitis in adults--a prospective study. | we have prospectively studied 27 adult patients attending the department of infectious diseases, göteborg, sweden, between october 1992 and october 1996 with a diagnosis of acute viral encephalitis. in addition to cerebrospinal fluid (csf) virus isolations and antibody analyses against herpes simplex virus, cytomegalovirus, varicella zoster virus, epstein-barr virus (ebv), enterovirus, adenovirus, tick-borne encephalitis virus, and mycoplasma, polymerase chain reaction test (pcr) to 5 viruses fr ... | 1998 | 9790126 |
| cytodifferentiating agents affect the replication of herpes simplex virus type 1 in the absence of functional vp16. | the herpes simplex virus type 1 (hsv-1) mutant in1814 encodes an altered form of the virion protein vp16 that is unable to transactivate immediate-early (ie) transcription. as a consequence of the mutation, in1814 initiates productive replication inefficiently after infection of tissue culture cells. previous studies showed that this defect could be overcome by the inclusion in the culture medium of hexamethylene bisacetamide (hmba), a compound that promotes the differentiation of murine erythro ... | 1998 | 9791032 |
| detection of microorganisms in vessel wall specimens of the abdominal aorta: development of a pcr assay in the absence of a gold standard. | a procedure in which the "invitrogen easy-dna" kit was followed by a silica-based method for the isolation of dna was developed for extraction of pcr-inhibitor-free dna from up to 300 mg of human vessel wall tissue. optimally designed pcr assays were developed for the detection of at least one infected cell in this amount of tissue. details of the procedure are given for the detection of dna of chlamydia pneumoniae, cytomegalovirus and herpes simplex virus type 1 and type 2 in human vessel walls ... | 1998 | 9795995 |
| pure populations of transduced primary human cells can be produced using gfp expressing herpes virus vectors and flow cytometry. | herpes simplex virus (hsv) has often been suggested as a vector for gene delivery to the nervous system although it is also capable of infecting many other cell types. hsv also has the ability to package large genetic insertions so the expression of multiple genes from a single virus is possible. here we show that a green fluorescent protein (gfp) expressing hsv1 vector can transduce two primary human cell types--quiescent human cd34+ hematopoietic progenitor cells and dendritic cells--which are ... | 1998 | 9797879 |
| caffeine inhibits paresthesia induced by herpes simplex virus through action on primary sensory neurons in rats. | herpetic infection causes paresthesia, including hypoalgesia, in humans and hypoalgesia in rats. this study was conducted to examine the effect of caffeine, which inhibits replication of herpes simplex virus type-1 (hsv) and affects several neuronal functions, on hsv-induced paresthesia in rats. hsv-induced hypoalgesia was suppressed by repeated treatment of unilateral hindpaw with 10% caffeine gel regardless of when the treatment was started. repeated treatment with acyclovir, an anti-hsv agent ... | 1998 | 9809669 |
| "suicide" gene for the control of graft-versus-host disease. | specific and conditional in vivo ablation of alloreactive donor t cells after allogeneic hematopoietic stem cell transplantation could significantly contribute to preventing and treating graft-versus-host disease (gvhd). the use of donor t cells expressing a "suicide" gene such as the thymidine kinase gene of the herpes simplex virus 1 (hs-tk) has the potential of achieving such a goal. ex vivo retroviral-mediated hs-tk gene transfer in human t cells as well as ganciclovir sensitivity of such ge ... | 1998 | 9814659 |
| adenoviral vectors efficiently target cell lines derived from selected lymphocytic malignancies, including anaplastic large cell lymphoma and hodgkin's disease. | we have hypothesized that adenoviral vectors might mediate gene transfer into cell lines derived from human lymphocytic malignancies, such as lymphoma, lymphocytic leukemia, and myeloma. a panel of 33 cell lines was studied for their ability to be transduced by an adenoviral (ad) vector encoding the escherichia coli beta-galactosidase gene (ad-betagal). a cytochemical assay and a flow cytometry assay both demonstrated that a subset of lymphocytic cell lines can be efficiently transduced by adeno ... | 1997 | 9815692 |
| translational control of viral and host protein synthesis during the course of herpes simplex virus type 1 infection: evidence that initiation of translation is the limiting step. | herpes simplex virus type 1 (hsv-1) infection induces the selective shut-off of host protein synthesis, other than ribosomal proteins, and the successive synthesis of viral proteins. because viral mrnas persist in the cytoplasm after viral protein synthesis has been inhibited, we hypothesized that viral gene expression may be under translational control. expression of genes encoding immediate early icp27, early dbp and late us11 proteins, together with glyceraldehyde-3-phosphate dehydrogenase (g ... | 1998 | 9820153 |