| evaluation of a new chromogenic medium, chromid™ vibrio, for the isolation and presumptive identification of vibrio cholerae and vibrio parahaemolyticus from human clinical specimens. | the aim of this study was to evaluate the performance of the chromid™ vibrio medium for the detection of vibrio cholerae and v. parahaemolyticus in stool and swab specimens in comparison with thiosulfate citrate bile salts sucrose (tcbs) medium. a total of 96 samples including 30 fresh stool, 32 stool, and 34 swab specimens originating from routine laboratories were tested. all samples were seeded on both media, the tcbs medium and the chromid vibrio, directly and after an enrichment step on alk ... | 2011 | 21484255 |
| the toxt-dependent methyl-accepting chemoreceptors acfb and tcpi contribute to vibrio cholerae intestinal colonization. | vibrio cholerae colonizes the human intestine and causes the acute diarrheal disease cholera. flagellar-mediated chemotaxis contributes to intestinal colonization as well as infectivity. the virulence-regulatory protein toxt activates transcription of the genes encoding the major virulence factors cholera toxin and toxin coregulated pilus. toxt additionally activates transcription of two genes, tcpi and acfb, located within the vibrio pathogenicity island predicted to encode methyl-accepting che ... | 2010 | 19929967 |
| n-terminal residues of the vibrio cholerae virulence regulatory protein toxt involved in dimerization and modulation by fatty acids. | the regulatory protein toxt is an arac family protein that is responsible for activating transcription of the genes encoding cholera toxin and toxin coregulated pilus, which are required for virulence by the human pathogen vibrio cholerae. the n terminus of toxt contains dimerization and regulatory elements, whereas the c terminus contains the dna binding domain. bile and long chain fatty acids negatively regulate toxt activity. utilizing a comprehensive alanine substitution mutant library of to ... | 2011 | 21673111 |
| suppressed induction of proinflammatory cytokines by a unique metabolite produced by vibrio cholerae o1 el tor biotype in cultured host cells. | vibrio cholerae o1 has two biotypes, el tor and classical, and the latter is now presumed to be extinct in nature. in carbohydrate-rich growth conditions, el tor biotype strains produce the neutral fermentation end product 2,3-butanediol (2,3-bd), which prevents accumulation of organic acids from mixed acid fermentation and thus avoids a lethal decrease in the medium ph, while the classical biotype strains fail to do the same. in this study, we investigated the inhibitory effect of 2,3-bd on the ... | 2011 | 21576340 |
| the rocks and shallows of deep rna sequencing: examples in the vibrio cholerae rnome. | new deep rna sequencing methodologies in transcriptome analyses identified a wealth of novel nonprotein-coding rnas (npcrnas). recently, deep sequencing was used to delineate the small npcrna transcriptome of the human pathogen vibrio cholerae and 627 novel npcrna candidates were identified. here, we report the detection of 223 npcrna candidates in v. cholerae by different cdna library construction and conventional sequencing methods. remarkably, only 39 of the candidates were common to both sur ... | 2011 | 21610211 |