| kinetics of intracellular killing of staphylococcus aureus and escherichia coli by human granulocytes. | | 1980 | 7000519 |
| bactericidal properties of lysosomal proteins obtained from human polymorphonuclear leucocytes (pmnl) examined in vitro. ii. morphological estimation of cells treated with lysosomal proteins. | the present paper is concerned with the action of lysosomal proteins isolated from granulocytes of human peripheral blood on gram-plus and gram-minus bacteria. differences in bacterial sensitivity to these proteins were found. the highest bactericidal activity was observed while bacteria from staphylococcus and escherichia were treated with lysosomal proteins. no structural changes in cells of bacteria from streptococcus and mycobacterium tuberculosis were observed. the sensitive bacteria cell w ... | 1980 | 7001810 |
| amino acid sequence and location of the three glycopeptides in the fc region of human immunoglobulin d. | | 1980 | 7008791 |
| effect of human saliva on glucose uptake by streptococcus mutans and other oral microorganisms. | we examined the effects of human whole salivary supernatant and parotid fluid on glucose uptake by streptococcus mutans, streptococcus sanguis, streptococcus mitis, actinomyces viscosus, staphylococcus aureus, and escherichia coli. the following three effects of saliva were observed: (i) inhibition of glucose uptake (s. mutans, s. sanguis), (ii) promotion of a transient, rapid (0 to 30 s) burst of glucose uptake (s. mutans, s. sanguis), and (iii) enhancement of glucose uptake (s. mitis, a. visco ... | 1981 | 7012014 |
| postantibiotic leukocyte enhancement: increased susceptibility of bacteria pretreated with antibiotics to activity of leukocytes. | exposure of escherichia coli and staphylococcus aureus to high levels of certain antibiotics for brief periods of time increases the susceptibility of the organisms to the antimicrobial action of normal human leukocytes. the degree of sensitization varies with the antibiotic used for the pretreatment of the bacteria and is most pronounced in pretreatment of e. coli with chloramphenicol. studies on the rates of phagocytosis and intracellular killing of e. coli indicate that the postantibiotic leu ... | 1981 | 7012999 |
| measurements of extracellular immunoglobulins as an index of b cell function. | we have developed a method for measuring the activation or suppression of b cells in culture by determination of immunoglobulins released into the extracellular fluid, using a conventional, nonisotopic assay. to avoid the interference of human serum proteins in the assays, cultures were established in medium supplemented with fetal calf serum. this caused some b cell activation, as reflected by the release of small amounts of immunoglobulins (1.5 to 0.7 microgram/ml igg, 0.8 to 1.4 microgram/ml ... | 1980 | 7019022 |
| tumor-associated bacteria capable of producing a human choriogonadotropin-like substance. | aerobic microorganisms were isolated and identified from 9 of 10 malignant tissues aseptically obtained from surgical patients. the organisms isolated are species commonly associated with the flora of the human body. when these cancer-associated organisms were grown in trypticase soy broth (bbl microbiology systems), a protein substance was isolated from the culture filtrates by acetone precipitation. the acetone precipitates of 12 of 14 organisms tested were positive when assayed by radioimmuno ... | 1981 | 7019090 |
| malakoplakia: evidence for an acquired disease secondary to immunosuppression. | a 44-year-old man was maintained on prednisone and azathioprine after renal transplantation. one and a half years after transplantation, he developed multiple perianal escherichia coli abscesses. these became chronic and resisted antibiotic therapy. histologically, the lesions were typical of malakoplakia. peripheral blood neutrophils and monocytes had impaired killing of staphylococcus aureus and e. coli in vitro. no abnormality of hexose monophosphate pathway activity, tetrazolium dye reductio ... | 1981 | 7020170 |
| application of partition affinity ligand assay (pala) in quick test for quantitation of staphylococcus aureus bacterial cells. | a novel immunochemical method for the quantitation of bacterial cells is described. the method, which is based on separation of bound from free reactants in aqueous two-phase systems has been studied both as direct and competitive binding assays, with a system consisting of intact cells of staphylococcus aureus and human immunoglobulin g molecules. to achieve high resolution, one of the reactants was modified so that the two reactants, the bacterial cells and the immunoglobulin molecules, occurr ... | 1981 | 7021681 |
| detection of igd-secreting human lymphocytes at the single cell level by a protein a plaque assay. | by the use of staphylococcus aureus protein a-coupled erythrocytes as target cells, and anti-ig antibodies as a developing serum, immunoglobulin-secreting cells can be detected as plaque-forming cells in a complement-dependent haemolytic plaque assay. this method is based on the fact that the fc portion of igg will bind to protein a molecules. using a rabbit anti-human igd immunoglobulin as a developing serum we were able to detect secretion of igd molecules from human cells. in a patient with a ... | 1981 | 7026092 |
| analysis of serum igg against treponema pallidum protein antigens in experimentally infected rabbits. | sensitive and highly specific radioimmunoprecipitation and gel electrophoreses-fluorography were used to monitor the serum igg response of experimentally infected rabbits against key protein antigens of treponema pallidum. the sera from six rabbits challenged intradermally (id) and six intratesticularly (it) with t pallidum were examined over a period of four months. a specific antibody response to most treponemal surface proteins (including those ligands implicated as mediating host-cell-surfac ... | 1981 | 7028207 |
| reduced hemoglobin as an inhibitor of human polymorphonuclear leukocyte bacterial killing. role of hemoglobin--oxygen tension in polymorphonuclear function. | the effect of reduced hemoglobin (hb) on in vitro human polymorphonuclear leukocyte (pmnl) bactericidal activity was investigated. addition of hb at physiologic concentrations and oxygen partial pressures (150 mg/ml, 35 torr) to pmnl bactericidal assays significantly inhibited the killing of blood culture isolates of escherichia and staphylococcus aureus. raising the oxygen partial pressure above 35 torr greatly decreased this inhibition. carboxy hemoglobin, produced by equilibrium of hg with ca ... | 1982 | 7034261 |
| protein a-like activity and streptococcal cross-reactions. | recognition of the protein a-like activity of some strains of group a streptococci has thrown doubt on much previous work suggesting antigenic cross-reactions between these streptococci and mammalian tissues. the strains used in our previous studies have now been examined by the mixed reverse passive antiglobulin reaction (mrpah) for the 'non-specific' absorption of purified fc portion of human igg. they were found to have only traces of activity. the strain of staphylococcus aureus used to cont ... | 1981 | 7039880 |
| expression of treponema pallidum antigens in escherichia coli. | treponema pallidum dna was cloned in a bacteriophage. clones were screened for expression of treponema pallidum antigens by an in situ radioimmunoassay on nitrocellulose, with the use of subsequent reactions with syphilitic serum and radioiodinated staphylococcus aureus protein a. one clone, which gave a strong signal, codes for at least seven antigens that react specifically with human antibodies to treponema pallidum. | 1982 | 7041257 |
| isoelectric points and surface hydrophobicity of gram-positive cocci as determined by cross-partition and hydrophobic affinity partition in aqueous two-phase systems. | thirty-nine streptococcal strains belonging to groups a, c, and g and 12 staphylococcal strains were investigated with respect to surface charge and hydrophobicity. isoelectric points of the bacteria were determined by cross-partition experiments in dextran-polyethylene glycol two-phase systems containing charged polymers. the results obtained indicate that group a, c, and g streptococci have isoelectric points of ph 3.75 +/- 0.15 standard deviation. staphylococci show an isoelectric point of ar ... | 1982 | 7042571 |
| the primary structure of human erythrocyte copper/zinc superoxide dismutase: cleavage with staphylococcus aureus protease, determination of the n-terminal blocking group and location of the disulfide bond. | results obtained after digestion of copper/zinc superoxide dismutase from human erythrocytes with s. aureus protease are described. in particular, peptides soluble in alkaline conditions proved essential for completing the determination of the primary structure of the enzyme; other peptides were important for establishing the amidation state of dicarboxylic amino acid residues and for confirming controversial sequences. the human enzyme is acetylated at the nh2 terminus and contains an intrasubu ... | 1982 | 7045030 |
| in vitro evaluation of centa, a new beta-lactamase-susceptible chromogenic cephalosporin reagent. | centa is a newly synthesized, beta-lactamase-labile, chromogenic cephalosporin reagent which changes color from light yellow (lambda maximum ca. 340 nm) to chrome yellow (lambda maximum ca. 405 nm) concomitant with hydrolysis of the beta-lactam ring. this compound offers promise as a diagnostic reagent comparable to other chromogens (padac and nitrocefin) for the early detection of beta-lactamase-producing clinical isolates, while retaining some antimicrobial effect against escherichia coli, kle ... | 1982 | 7047560 |
| [possibilities of resuming breast feeding after suppurative or inflammatory breast diseases]. | | 1982 | 7070913 |
| characterization of the binding domains in the fragments cleaved by cathepsin g from human plasma fibronectin. | | 1982 | 7075587 |
| high-level potentiation of lysostaphin anti-staphylococcal activity by lysozyme. | the purpose of this study was to determine whether lysostaphin would enhance its anti-staphylococcal efficacy in combination with lysozyme. minimum inhibitory concentrations (mics) of lysostaphin and lysozyme were separately determined for 41 strains belonging to 10 different species of human staphylococci. lysozyme was virtually inactive and showed mics of 15 mg/ml. on the contrary, all strains were susceptible to lysostaphin and showed mics ranging from 2.5 to 60 micrograms/ml for the differen ... | 1982 | 7081976 |
| anti-poly(glycerolphosphate) in human sera. | ultrasonic extracts from staphylococci contained red cell-sensitizing poly(glycerolphosphate)teichoic acid antigen. the antigen may contribute to the variability of precipitin reactions used in the diagnosis of staphylococcus aureus infection. | 1982 | 7107847 |
| enterotoxin production by staphylococcus aureus strains isolated from humans, foods and animals in malaysia. | enterotoxin production by strains of staphylococcus aureus isolated from human, food and animal sources was investigated. of the 130 isolates studied, 27 (20.8%) were found to be enterotoxigenic. the most common enterotoxin detected from human sources was enterotoxin c whereas enterotoxin b occurred more frequently in staphylococcal strains of food origin. the 2 enterotoxigenic strains, from animals isolated from a dog and a goat, produced enterotoxins a and c, respectively. enterotoxin e was no ... | 1982 | 7112212 |
| fixed protein a-containing staphylococci as solid-phase immunoadsorbents. | staphylococcus aureus cowan i containing protein a were rapidly fixed in hot 5% (w/v) trichloroacetic acid (tca) for 6 min in order to remove the negatively charged cell wall polymer teichoic acid. this resulted in a stable igg adsorbent able to bind 1.4 mg of human igg/ml 10% (v/v) suspension for at least 5 months. this igg binding ability is 25% less than for formalin-fixed bacteria, which were stable for at least 1 year. preincubation of the bacterial adsorbents in 0.5% (v/v) tween 20 after f ... | 1982 | 7119455 |
| the effect of exposure of staphylococcus aureus to penicillin on susceptibility to the bactericidal activity of human leukocytes. | the effect of brief exposure of staphylococci to penicillin upon susceptibility to leukocidal activity was studied. antibiotic pretreated staphylococci were incubated with normal human leukocytes and viability of the bacteria was determined. penicillin pretreated staphylococci were more susceptible to killing by leukocytes than untreated control bacteria. the extent of sensitization varied between different strains of staphylococci. when staphylococci were exposed simultaneously to leukocytes an ... | 1982 | 7133758 |
| [recurrent toxic shock syndrome in a man after surgery for a pilonidal cyst]. | | 1982 | 7135618 |
| [studies on bacterial aerosol. part 7. dnase activity produced by airborne staphylococci and resident staphylococci on human bodies]. | | 1982 | 7166879 |
| circulation of enterotoxigenic strains of staphylococcus aureus in humans and their environment. | in the study of eight groups of staphylococci covering 870 strains of staphylococcus aureus, isolated from humans and ten kinds of animals, it was found that a total of 271 strains [31.4%] produced some of the types of staphylococcal enterotoxin [se]. with the exception of 34 strains isolated from staphylococcal enterotoxicosis the other strains were unrelated to this disease. while among healthy persons 14% [students]---57% [employees of food industry undertakings] enterotoxigenic strains were ... | 1980 | 7190989 |
| plasma membrane-associated antigens on tumor cells derived from transitional-cell carcinoma of the human urinary bladder. ii. identification at the molecular level of plasma membrane-associated antigens. | the surface proteins of seven human cell lines (three bladder carcinomas (tcc), two normal urothelial lines, one colon carcinoma, and one malignant melanoma) were labelled with 125i by the glucose oxidase-lactoperoxidase technique. plasma membranes of the cells were isolated and analysed by sodium dodecyl sulphate electrophoresis (sds-page). when analysed under reducing conditions by staining with protein stain, approximately 45 distinct membrane polypeptides were detected in all membrane prepar ... | 1980 | 7203713 |
| binding of radiolabeled staphylococcus aureus delta-toxin to human erythrocytes. | the addition f [3h]isoleucine to a chemically defined medium resulted in the production of delta-toxin (dt) with a high specific radioactivity (0.47 microci/mg). the purified tritium-labeled toxin ([3h]dt) was found to migrate in sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a single band with a molecular weight of 1,600. upon electrofocusing, [3h]dt yielded one major peak (pi = 5.90) and two minor peaks (pi = 5.10, 6.95) of radioactivity. the percentage of [3h]dt associated with ... | 1981 | 7228396 |
| chemical and carbon-13 nuclear magnetic resonance studies of the blood group m and n active sialoglycopeptides from human glycophorin a. | the nh2-terminal sialoglycopeptides from human erythrocyte glycophorin a have been obtained by specific proteolytic cleavage and gel filtration chromatography. by cyanogen bromide cleavage, a glycosylated octapeptide was obtained from blood group m donors having an amino acid composition and 13c nmr spectrum consistent with the structure (formula: see text) was demonstrated. by staphylococcus aureus protease cleavage, a glycosylated pentapeptide was obtained from n donors having the same structu ... | 1981 | 7240172 |
| abnormalities in membrane phospholipid organization in sickled erythrocytes. | in contrast to the wealth of information concerning membrane phospholipid asymmetry in normal human erythrocytes, very little is known about membrane phospholipid organization in pathologic erythrocytes. since the spectrin-actin lattice, which has been suggested to play an important role in stabilizing membrane phospholipid asymmetry, is abnormal in sickled erythrocytes, we determined the effects of sickling on membrane phospholipid organization. we used two enzymatic probes: been venom phosphol ... | 1981 | 7240412 |
| [toxic-shock syndrome. three cases (author's transl)]. | several cases of toxic shock syndrome (t.s.s) have been recently reported from the u.s.a. clinical features of this new syndrome include fever, desquamative scarlatiniform rash, hypotension and involvement of central nervous system, liver, kidney and muscles. more than 90% of cases are women with staphylococcic vaginitis using tampons during menstruations. a toxin produced by staphylococcus aureus is thought to be the causative agent, because the germ has been isolated in local (vaginal, pharyng ... | 1981 | 7267317 |
| [addition to the scheme of ecologic variants of staphylococcus aureus]. | altogether 55 s. aureus strains were isolated from the nose and skin of 2 monkey species (cercopithecus sabaeus and macaca fascicularis). the strains were studied in 19 biological tests. 45 of these strains were found to differ in their biological properties from the ecological s. aureus variants described in literature. of these grounds the strains were classified with the new ecovar simiae comprising 2 subvariants. the strains of the ecovar simiae coagulated human plasma, did not coagulate bov ... | 1981 | 7282191 |
| [resistance development in human staphylococcus aureus due to the use of oxytetracycline and tylosin in industrialized swine production units]. | staphylococcus aureus strains from human nasal carriers without vocational contact with pigs could be differentiated from the majority of strains obtained from pathologically affected pigs by determination of host-specific-variety, according to meyer (1966) and witte et al. (1977). less unambiguous findings regarding site variability were recorded from strains in nasal mucosa of pig-handling personnel, clinically intact pigs on farms, and slaughtered pigs. antibiotics, such as oxytetracycline an ... | 1981 | 7283644 |
| antibody levels to bacterial peptidoglycan in human sera during the time course of endocarditis and bacteremic infections caused by staphylococcus aureus. | sera from patients with endocarditis and bacteremia due to staphylococcus aureus were compared for peptidoglycan-binding capacity with those from normal blood donors. those patients treated with beta-lactam antibiotics had higher antigen-binding levels than normal donors and patients treated exclusively with vancomycin (p less than 0.01). the factor responsible for this activity was purified by affinity chromatography from a normal donor and shown to be an immunoglobulin. specificity studies ind ... | 1981 | 7287182 |
| comparison of mechanisms of interaction between protein a from staphylococcus aureus and human monoclonal igg, iga and igm in relation to the classical fc gamma and the alternative f(ab')2 epsilon protein a interactions. | four purified human monoclonal igg, iga and igm proteins were tested for their inhibitory effect on the binding of protein-a-reactive 125i-ige and 125i-fc gamma, respectively, to protein-a-sepharose. only igg myeloma proteins significantly inhibited the binding of 125i-fc gamma to protein-a-sepharose, whereas most, but not all, myeloma proteins, irrespective of their immunoglobulin class and with varying efficiency, inhibited the binding of protein-a-reactive 125i-ige to protein-a-sepharose. the ... | 1981 | 7302528 |
| variability in the degree of opsonization and phagocytosis of strains of staphylococcus aureus isolated from patients with disseminated intravascular coagulation. | patients with septicemia due to staphylococcus aureus occasionally manifest dic. we studied the ability of organisms isolated from 30 patients with s. aureus (5 with dic) to be opsonized and phagocytosed by normal human pmns in the presence of normal human serum chelated with mgegta as a possible measure of alternative complement pathway activation. by this assay, nine s. aureus strains were found to be opsonized at 50% level or greater. five of those organisms were isolated from the five patien ... | 1981 | 7310231 |
| radioimmunoassay of human adenosine deaminase. | | 1981 | 7321887 |
| classification of macrolide-lincosamide-streptogramin-b-type antibiotic resistance determinants. | we have examined the relationship between the genes specifying resistance towards macrolid-lincosamide-streptogramin-b-type (mls) antibiotics from group d and h streptococci, staphylococcus aureus, bacillus licheniformis, and bacteroides fragilis by dna annealing. we detected homology between the mls resistance determinants from the gram-positive cocci (streptococci and staphylococci) but not between these and the genes from b. licheniformis and b. fragilis. based on these dna-dna hybridizations ... | 1981 | 7332186 |
| enterotoxin production by staphylococcus aureus related to the origin of the strains. | the production of enterotoxins by strains of staphylococcus aureus of human and animal origin seems to be common. 104 out of 170 strains (61%) produced one or more of the a, b, and c enterotoxins. strains from cow and milk often produced enterotoxin c, and enterotoxin a producing strains were mainly isolated from dogs. human food poisoning seemed in our material to be induced by enterotoxin a producing strains. | 1981 | 7336929 |
| the clinical spectrum of toxic shock syndrome. | toxic shock syndrome (tss) is a recently recognized acute multisystem illness that may recur. epidemiologic links with menstruation and use of tampons have been identified. we report the cases of seven patients (six women and one man), 12 to 31 years old, seen over nine months, who met the criteria for tss. four were menstruating at onset. all had hypotension, fever, erythematous rash and distal desquamation. a prodrome of myalgias and diarrhea occurred in all patients. clinical features of the ... | 1981 | 7340115 |
| mammalian bite wounds. | clinical data were collected prospectively from a series of 160 patients presenting with mammalian bite wounds. anaerobic and aerobic cultures were prepared from sterile swabs placed in 65 bite wounds prior to cleansing. infection was noted in 11 of 22 cat bites, six of 37 human bites, three of 80 dog bites, and in none of the 21 bites caused by other mammals. pasteurella multocida was recovered from six infected cat and dog bites, all of which developed infection within 24 hours of injury. stap ... | 1980 | 7356213 |
| solid-phase radioimmunoassay with protein-a-bearing staphylococcus aureus cells used to assay a protein (ferritin) and a hapten (digoxin). | we compared use of protein-a-containing staphylococcus aureus bacteria with conventional ammonium sulfate precipitation and second-antibody methods of separating bound and free antigen in the radioimmunoassay of a hapten (digoxin) and protein (ferritin) in human sera. in each case, values obtained with the heat-killed, formalin-fixed bacteria correlated well with those found by established methods. no matrix effects were detected in either hapten or protein measurements. because of the affinity ... | 1980 | 7356569 |
| a new haemolysin from staphylococcus aureus which lyses horse erythrocytes. | a new haemolysin from staphylococcus aureus produced opaque zones of haemolysis on horse blood agar but did not lyse equine erythrocytes suspended in phosphate-buffered saline. the haemolysin was not neutralized by normal rabbit serum and was distinct from alpha-, beta- and delta-haemolysins as well as human leucocidin. partially purified preparations produced erythema when injected intradermally into rabbit skin. | 1980 | 7365453 |
| identity and interspecific transfer of gentamicin-resistance plasmids in staphylococcus aureus and staphylococcus epidermidis. | the hypothesis that emergence of gentamicin-resistant strains of staphylococcus aureus and staphylococcus epidermidis in a neonatal special care nursery was the result of transfer of a single plasmid between these two species was examined. in experiments with mixtures of staphylococci, either in mixed cultures or on human skin, isolates of s. aureus and s. epidermidis transferred their gentamicin-resistance plasmids both intra- and interspecifically. by electron microscopy, the molecular masses ... | 1980 | 7391616 |
| clinical and pharmacokinetic evaluation of parental cefoxitin in infants and children. | thirty-two infants and children ranging in age from 3 to 151 months (mean, 26 months) were treated with parenteral cefoxitin (150 mg/kg per day). ten patients with isolates of haemophilus influenzae (six with cellulitis, two with arthritis, and two with mastoiditis), four with staphylococcus aureus (one with lymphadenitis, one with septicemia, and two with abscess), and three patients with streptococcus pneumoniae (one each with cellulitis, abscess, and arthritis), were clinically and bacteriolo ... | 1980 | 7396456 |
| [hemoglobins, xxxii. analysis of the primary structure of the monomeric hemoglobin ctt viia (erythrocruorin) or chironomus thummi thummi, diptera (author's transl)]. | the dimeric hemoglobin ctt viia (erythrocruorin) was isolated from the hemolymph of the larva from chironomus thummi thummi and purified by preparative polyacrylamide gel electrophoresis. peptides obtained by limited tryptical digestion were sequenced by automatic edman degradation. for the elucidation of the sequence in the c-terminal region of the chain, additional cleavages with proteinase of staphylococcus aureus and chymotrypsin were necessary. ctt viia is compared with human beta-chains an ... | 1980 | 7399413 |
| [detection of "staphylococcus aureau" protein a on selective culture media (author's transl)]. | production of protein a by 100 various animal strains and 106 human strains of staphylococcus aureus has been detected by conditioned haemagglutination after growth on seven different culture media: agar medium at n degrees 2 and columbia agar with and without nalidixic acid (10.8 micrograms/ml), baird-parker, chapman and tellurite-glycine-agar media. rate of protein a-producing strains is as high on nalidixic acid media as on the same media without antibiotic. baird-parker, chapman and tellurit ... | 1980 | 7425451 |
| experimental osteomyelitis due to staphylococcus aureus or pseudomonas aeruginosa: a radiographic-pathological correlative analysis. | a previously-described experimental model of bacterial osteomyelitis was used to investigate systematically the sequential radiographic and histopathological changes in the tibias of rabbits infected with either staphylococcus aureus or pseudomonas aeruginosa. the radiographic changes induced by both organisms were progressive, increasing in severity from the first to the fourth week after infection. the severity and extent of radiographic changes, especially that of bond destruction, were signi ... | 1980 | 7426395 |
| modulation of human polymorphonuclear leukocyte chemotaxis by leukocyte extracts, bacterial products, inflammatory exudates, and polyelectrolytes. | human polymorphonuclear leukocytes (pmn) chemotaxis was tested during exposure to leukocyte and platelet extracts, a variety of polyelectrolytes, inflammatory exudates, and bacterial products. the chemoattractants employed were either zymosan-activated serum or supernatant from autolyzed staphylococcus aureus. chemotaxis to both chemoattractants was markedly inhibited by leukocyte and platelet extracts; inflammatory exudates; anionic polyelectrolytes, dna, hyaluronic acid, liquoid; and by cation ... | 1980 | 7429609 |
| mitogenic responsiveness of mouse, rat and human lymphocytes to staphylococcus aureus cell wall, teichoic acid, and peptidoglycan. | the mitogenic activity of staphylococcus aureus peptidoglycan (pg), teichoic acid (ta) and cell wall (cw, a pg-ta complex) for mouse, rat and human lymphocytes was determined by measuring dna synthesis. at optimal concentrations pg was the most potent mitogen, inducing maximum stimulation of mouse spleen lymphocytes on day 2 of culture, rat spleen lymphocytes on day 3, rat lymph node cells on day 2, human peripheral blood lymphocytes on day 5, and human cord blood lymphocytes on day 4. cw was le ... | 1980 | 7429652 |
| platelet interaction with bacteria. vi. contrasting the role of fibrinogen and fibronectin. | the roles of fibrinogen and fibronectin were contasted in the responses of human platelets to staphylococcus aureus and collagen. congenital afibrinogenemic (ca) platelets and washed normal platelets had delayed aggregation due to a prolonged latent phase in response to contact with the bacteria when fibrinogen was absent from the suspending media. when a physiologic level of human fibrinogen was added to the ca platelets in autologous plasma or to the washed platelets in balanced saline, the ti ... | 1980 | 7435500 |
| effect of extracellular serum in the stimulation of intracellular killing of streptococci by human monocytes. | this study shows that the intracellular killing of streptococcus pyogenes, streptococcus faecalis, and streptococcus pneumoniae by human monocytes is stimulated by the extracellular presence of both heat-stable and heat-labile serum factors. a similar kind of stimulation of monocytes has been described in respect of catalase-positive microorganisms. however, killing of these bacteria is negligible in the absence of extracellular serum factors, whereas a large proportion of the ingested catalase- ... | 1980 | 7439986 |
| bactericidal activity of cerumen. | freshly collected cerumen (dry form) suspended at a concentration of 3% in glycerol-sodium bicarbonate buffer showed bactericidal activity against some strains of bacteria tested. this suspension reduced the viability of haemophilus influenzae, escherichia coli k-12, and serratia marcescens by more than 99%, whereas the viability of two pseudomonas aeruginosa isolates, e. coli k-1, streptococcus, and two staphylococcus aureus isolates of human origin was reduced by 30 to 80%. the results support ... | 1980 | 7447422 |
| sensitivity of staphylococci to fatty acids: novel inactivation of linolenic acid by serum. | the inhibition of coagulase-negative staphylococci and staphylococcus aureus of human or animal origin by most free fatty acids was similar, but coagulase-positive staphylococci were sensitive and coagulase-negative cultures were resistant to linolenic acid. animal strains of s. aureus were more sensitive to linolenic acid than were human strains. these differences were reflected in the relative abilities of the three categories of strains to survive on human skin. the antibacterial effects of 2 ... | 1981 | 7463466 |
| [combination therapy with arbekacin and fosfomycin against postoperative severe mixed-pneumonia of mrsa in primary lung cancer patients]. | we experienced successful treatment of postoperative severe pneumonia of methicillin-resistant staphylococcus aureus (mrsa) with combination therapy of arbekacin (abk) and fosfomycin (fom) in three lung cancer patients. case 1 was a advanced age of seventy-nine man who had had right upper lobectomy. case 2 was a 61-year-old man who had had left lower lobectomy and extended bilateral mediastinal lymph-node dissection through the median sternotomy. and case 3 was a 59-year-old man who had suffered ... | 1995 | 7474582 |
| cd27-cd70 interactions regulate b-cell activation by t cells. | cd27, a member of the tumor necrosis factor (tnf) receptor family, binds to its ligand cd70, a member of the tnf family, and subsequently induces t-cell costimulation and b-cell activation. cd27 is expressed on resting t and b cells, whereas cd70 is expressed on activated t and b cells. utilizing transfected murine pre-b-cell lines expressing human cd27 or cd70, we have examined the effect of such transfectant cells on human b-cell igg production and b-cell proliferation. we show that the additi ... | 1995 | 7479974 |
| unconventional b-cell antigens and human immune repertoires. | | 1995 | 7486544 |
| bactericidal killing activities of cefepime, ceftazidime, cefotaxime, and ceftriaxone against staphylococcus aureus and beta-lactamase-producing strains of enterobacter aerogenes and klebsiella pneumoniae in an in vitro infection model. | cefepime (cp) is a new injectable cephalosporin with a broad spectrum of activity and stability against common chromosomally and plasmid-mediated beta-lactamases. the bactericidal activities of cp, ceftazidime (cz), cefotaxime (ctx), and ceftriaxone (cax) against reference and clinical strains of staphylococcus aureus, an isogenic pair of enterobacter aerogenes strains (wild type and a cz-resistant derepressed mutant), and a klebsiella pneumoniae isolate possessing a tem-10 beta-lactamase were i ... | 1995 | 7486916 |
| the effect of zinc on bacterial phagocytosis, killing and cytoprotection in human polymorphonuclear leucocytes. | an in vitro study examining the effects of zinc treatment on human pmn cell phagocytosis and killing of staphylococcus aureus and staphylococcus epidermidis and the cytoprotection of zinc against staphylococcal toxins. phagocytosis was studied by transmission electron microscopy using different microbiological techniques, one of which was designed to follow the kinetics of bacterial killing. no effect was found on phagocytosis and bacterial killing. the cytotoxic effects of a crude toxin and an ... | 1995 | 7488384 |
| enhancement of specific immunoglobulin production in scid-hu-pbl mice after in vitro priming of human b cells with superantigen. | priming of human mature b cells in vitro with staphylococcal enterotoxin a (sea) prior to transplantation of the b cells into severe combined immunodeficiency (scid) mice, together with human t-helper cells, resulted in higher and more uniform concentrations of serum igg in the mice. this indicated that a large number of b cells had become activated, which was supported by the finding that sea priming resulted in production of immunoglobulin displaying a more normal kappa/lambda ratio than was o ... | 1995 | 7490122 |
| inhibition of activation of human t lymphocytes by the complement c1 esterase inhibitor. | the major histocompatibility complex (mhc) class i molecules have been shown to be substrates to both of the complement c1 esterases. the effect of a c1 esterase-mediated cleavage of the mhc class i molecules on the activation process of lymphocytes was investigated by including the complement c1 esterase inhibitor (c1-inh) in the medium during activation of human peripheral lymphocytes by staphylococcal enterotoxin a (sea). the c1-inh was shown to inhibit the activation of both cd4+ and cd8+ ce ... | 1995 | 7490133 |
| corynebacterium seminale sp. nov., a new species associated with genital infections in male patients. | we studied 12 coryneform isolates having similar biochemical profiles which did not permit their assignment to any recognized taxa. human semen was the source for seven of these strains, whereas the other strains were isolated from urethra, urine, and blood specimens of adult male patients. these bacteria were found in significant quantities (10(4) to 10(5) cfu/ml) in semen specimens from infertile male patients with the diagnosis of prostatitis. these strains had characteristics of the genus co ... | 1995 | 7494009 |
| contribution of phagocytic cells and bacteria to the accumulation of technetium-99m labelled polyclonal human immunoglobulin at sites of inflammation. | the purpose of this study was to assess the contribution of phagocytic cells and bacteria to the accumulation of technetium-99m labelled polyclonal human immunoglobulin (hig) at sites of inflammation. mice were intraperitoneally injected with staphylococcus aureus (sa animals), with heat-inactivated newborn calf serum (nbcs, to mimic a non-bacterial inflammation) or with physiological saline (controls); 1 h thereafter they received hig. at various intervals after the administration of hig the mi ... | 1995 | 7498225 |
| collagen-like complement component c1q is a membrane protein of human monocyte-derived macrophages that mediates endocytosis. | the collagen-like c1q molecule, a subcomponent of the first component of complement, c1, is synthesized by macrophages (m phi). previously, we have demonstrated that c1q is a membrane protein of guinea pig peritoneal macrophages (m phi). to extend this observation as a general biologic characteristic of m phi, we investigated human (hu) monocyte-derived m phi. interestingly, surface labeling with the biotin derivative sulfosuccinimidyl-6-(biotinamido)-hexanoate of m phi, freshly isolated monocyt ... | 1995 | 7499868 |
| sulfatides trigger increase of cytosolic free calcium and enhanced expression of tumor necrosis factor-alpha and interleukin-8 mrna in human neutrophils. evidence for a role of l-selectin as a signaling molecule. | sulfatides have been established recently as ligands for l-selectin, and we investigated whether they trigger transmembrane signals through ligation of l-selectin. we found that sulfatides trigger the increase of cytosolic free calcium in neutrophils and that this effect was strictly dependent on sulfation of the galactose ring, as non-sulfated galactocerebrosides were not stimulatory. chymotrypsin and phorbol 12-myristate 13-acetate treatment of neutrophils caused shedding of l-selectin, but no ... | 1994 | 7508438 |
| inhibition of human b lymphocyte cell cycle progression and differentiation by rapamycin. | in this study, we have analyzed the effects of the immunosuppressive agent rapamycin on the activation of highly purified normal human b lymphocytes. when the polyclonal activators staphylococcus aureus (sa) and soluble cd40 ligand (cd40l) were used to stimulate b cells, rapamycin inhibited both interleukin 2 (il2)-dependent and -independent proliferation, as well as il2-dependent differentiation into antibody-secreting cells. cell cycle analysis indicated that rapamycin inhibited the progressio ... | 1994 | 7517796 |
| cd1+ human thymocytes proliferate in response to superantigen staphylococcal enterotoxin b1. | exposure of human thymocytes to superantigens results in the deletion of thymocytes expressing specific tcr-v beta genes. the factors that contribute to this deletion may relate to the inherent nature of the t cell at a given stage of development. in this paper, we demonstrate that cd1+ human cortical thymocytes are capable of proliferating in response to a bacterial superantigen (staphylococcal enterotoxin b (seb)) in the presence of autologous cd2-/low thymic apcs. phenotypic analysis of the r ... | 1994 | 7519639 |
| gene therapy for cystic fibrosis using e1-deleted adenovirus: a phase i trial in the nasal cavity. the university of north carolina at chapel hill. | cystic fibrosis (cf) is an autosomal recessive disease that reflects mutations in the cftr gene. multiple mutations in this gene have been detected that lead to a protein (cftr) that is abnormally metabolized, dysfunction, or both. the full spectrum of the activities of the gene product have not been defined, but it is clear that cftr can act as a camp-regulated cl- channel. this type of defect is consistent with the physiologic characterization of cf epithelia, which has revealed abnormalities ... | 1994 | 7519885 |
| soluble peptidoglycan-induced monokine production can be blocked by anti-cd14 monoclonal antibodies and by lipid a partial structures. | we have investigated the interaction of soluble peptidoglycan (spg), in comparison with lipopolysaccharide (lps), with human mononuclear cells (mnc) by determining the capacity of spg to induce interleukin-6 (il-6) and il-1 release. in addition, we investigated the modulation of their interaction by anti-cd14 monoclonal antibody and by partial structures of lps. we found that spg, like lps, was able to induce il-6 and il-1 production by mnc. however, dose-response experiments revealed that at le ... | 1994 | 7523297 |
| reconstituted high-density lipoprotein neutralizes gram-negative bacterial lipopolysaccharides in human whole blood. | we have tested hypotheses relating lipoprotein structure to function as measured by the relative ability to neutralize endotoxin by comparing natural human lipoproteins, a chemically defined form of reconstituted high-density lipoprotein (r-hdl), and a lipid emulsion (intralipid). the human whole-blood system was used as an in vitro model of lipopolysaccharide (lps) binding protein and cd14-dependent activation of cytokine production. when lipoproteins were compared on the basis of protein conte ... | 1995 | 7528733 |
| expression and regulation of cd30 ligand and cd30 in human leukemia-lymphoma cell lines. | the cd30 antigen was originally described as a specific surface marker for hodgkin's lymphoma. recent work established cd30 as a member of the tumor necrosis factor/nerve growth factor receptor superfamily whose ligand (cd30l) has also been cloned and expressed; cd30l is active as membrane-bound type ii glycoprotein. here, cd30l mrna expression was studied in a panel of 102 continuous human leukemia-lymphoma cell lines and was found only in four burkitt lymphoma, one burkit-type acute lymphoblas ... | 1994 | 7528856 |
| purification and characterization of two potent heat-stable protein inhibitors of protein phosphatase 2a from bovine kidney. | two heat-stable protein inhibitors of protein phosphatase 2a (pp2a), tentatively designated i1pp2a and i2pp2a, have been purified to apparent homogeneity from extracts of bovine kidney. the purified preparations of i1pp2a exhibited an apparent m(r) approximately 30,000 and 250,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel permeation chromatography on sephacryl s-300, respectively. in contrast, the purified preparations of i2pp2a exhibited an apparent m(r) ... | 1995 | 7531497 |
| recovery of human fibroblasts from attack by the pore-forming alpha-toxin of staphylococcus aureus. | when applied at low concentrations (< 10 micrograms/ml), staphylococcal alpha-toxin generates a small channel in keratinocyte and lymphocyte membranes that permits selective transmembrane flux of monovalent ions. here we show that a moderate concentration (1-50 micrograms/ml) of alpha-toxin similarly produces a small pore in membranes of human fibroblasts. this process leads to rapid leakage of k+ and to a drop in cellular atp to 10-20% of normal levels in 2 h. in the presence of medium suppleme ... | 1994 | 7535374 |
| expression of adhesion molecules on human granulocytes after stimulation with helicobacter pylori membrane proteins: comparison with membrane proteins from other bacteria. | type b gastritis in its active form is characterized by a dense infiltration of the lamina propria with granulocytes. since the bacterium helicobacter pylori does not invade the epithelial barrier, a signaling pathway chemoattractive for granulocytes must exist across this mucosal boarder. one possible mechanism tested was whether granulocytes are directly activated by water-soluble membrane proteins (wsp) from h. pylori. these findings were compared with the effects of wsp from other bacteria ( ... | 1995 | 7540595 |
| evaluation of a method for measurement of intracellular killing of staphylococcus aureus in human neutrophil granulocytes. | a modified method for measurement of intracellular killing of staphylococcus aureus in human neutrophil granulocytes is described. after phagocytosis of s. aureus the extracellular bacteria were sufficiently removed by repeated centrifugations and washings of the granulocytes. the optimal conditions for incubation of granulocytes for measurement of intracellular killing were found to be 37 degrees c in the presence of 5% co2. under these conditions, stable ph, the viability and the capacity of t ... | 1995 | 7546649 |
| new thrombolytic agents and strategies. | despite their widespread use in patients with acute myocardial infarction, all currently available thrombolytic agents suffer from a number of significant limitations, including resistance to reperfusion, the occurrence of acute coronary reocclusion, and bleeding complications. several lines of research towards improvement of thrombolytic therapy are being explored, including strategies to enhance the fibrinolytic potency of plasminogen activators and to improve conjunctive antiplatelet or antit ... | 1995 | 7549072 |
| immunotherapy of human colon cancer by antibody-targeted superantigens. | t lymphocytes generally fail to recognize human colon carcinomas, suggesting that the tumour is beyond reach of immunotherapy. bacterial superantigens are the most potent known activators of human t lymphocytes and induce t cell cytotoxicity and cytokine production. in order to develop a t-cell-based therapy for colon cancer, the superantigen staphylococcal enterotoxin a (sea) was given tumour reactivity by genetic fusion with a fab fragment of the monoclonal antibody c242 reacting with human co ... | 1995 | 7553685 |
| staphylococcal scalded skin syndrome. | staphylococcal scalded skin syndrome (ssss) is a recognised clinical entity that affects primarily the very young and, in rare cases, the very old or the immunocompromised. koch's postulates have been fulfilled in that: (i) staphylococcus aureus is isolated from every case; (ii) s. aureus can reproduce the syndrome in an experimental animal model; (iii) a specific extracellular toxin can reproduce the syndrome; and (iv) antibody to the toxin can protect experimental animals. although exfoliative ... | 1995 | 7562995 |
| [penetration of ceftriaxone (1 or 2 gr intravenous) into mediastinal and cardiac tissues in man]. | ceftriaxone penetration into heart tissues (valves, myocardium, auricles and pericardium) and mediastinal tissues (fat and sternal bone) was evaluated after two regimens of ceftriaxone administration. ten patients were given 1,000 g intravenously of ceftriaxone 30 min. before anesthesia. ten other patients received the same dose and then a second 1,000 mg dose at the time of initiation of cardiopulmonary bypass. similar and very satisfactory ceftriaxone tissue penetrations were observed in both ... | 1995 | 7567130 |
| thrombolytic agents in development. | the quest continues for thrombolytic agents with a higher thrombolytic potency, specific thrombolytic activity and/or a better fibrin selectivity. several lines of research towards improvement of thrombolytic agents are being explored, including the construction of mutants and variants of plasminogen activators (pas), chimaeric pas, conjugates of pas with monoclonal antibodies, and pas from animal or bacterial origin. some of these new thrombolytic agents have shown promise in animal models of v ... | 1995 | 7588087 |
| inhibition of expression of cyclin a in human b cells by an immunosuppressant mizoribine. | mizoribine has been used to prevent rejection of organ allografts in humans and in animal models. recent clinical trials have demonstrated its efficacy in rheumatoid arthritis and lupus nephritis, in which abnormalities of b cell functions are also involved. we therefore examined the effects of mizoribine on the in vitro function of human b cells. igm production was induced from highly purified b cells obtained from healthy donors by stimulation with staphylococcus aureus cowan i (sa) plus il-2. ... | 1995 | 7594527 |
| [successful treatment of an infected pseudoaneurysm of the ascending aorta with omental transfer--a case report]. | the formation of pseudoaneurysm is an uncommon complication of after coronary artery bypass grafting (cabg). we report a 66-year-old man in whom an anastomotic pseudoaneurysm of the ascending aorta derived from mediastinitis after repeat cabg. at operation, the pseudoaneurysm was revealed to be involved in the proximal anastomotic site of a saphenous vein graft to the rca. the aneurysm was resected and the defect was repaired with woven dacron patch under deep hypothermic circulatory arrest. in ... | 1995 | 7594853 |
| expression and biological activities of bovine interleukin 4: effects of recombinant bovine interleukin 4 on t cell proliferation and b cell differentiation and proliferation in vitro. | interleukin 4 (il-4) is a pleotropic cytokine affecting a wide range of cell types in both the mouse and the human. these activities include regulation of the growth and differentiation of both t and b lymphocytes. the activities of il-4 in nonprimate, nonmurine systems are not well established. herein, we demonstrate in the bovine system that il-4 upregulates production of igm, igg1, and ige in the presence of a variety of costimulators including anti-igm, staphylococcus aureus cowan strain i, ... | 1995 | 7606798 |
| role of protein kinase c isozymes in fc gamma receptor-mediated intracellular killing of staphylococcus aureus by human monocytes. | intracellular killing of staphylococcus aureus by human monocytes after cross-linking fc gamma r is known to be a phospholipase c (plc)-dependent process. activation of plc leads to the formation of second messengers that synergistically activate protein kinase c (pkc). the aim of this study was to obtain more insight into the role of pkc in fc gamma r-mediated killing process. pkc inhibitors h-7 and staurosporine markedly suppressed the killing of s. aureus by monocytes stimulated by cross-link ... | 1995 | 7608554 |
| essential binding of lukf of staphylococcal gamma-hemolysin followed by the binding of h gamma ii for the hemolysis of human erythrocytes. | staphylococcal gamma-hemolysin consists of two protein components, lukf and h gamma ii. this report clarifies the order of binding of the two components to human erythrocytes for the hemolytic activity of the toxin. the initial binding of lukf to the human erythrocytes is essential for the subsequent binding of h gamma ii to the same cells, with the formation of a heat-undissociable protein of about 100 kda containing lukf and h gamma ii. | 1995 | 7613012 |
| the absence of evidence of staphylococcal toxin involvement in the pathogenesis of kawasaki disease. | to detect a causative superantigen and to clarify a possible role for staphylococci in kawasaki disease (kd), culture supernatants of individual bacterial isolates from 11 acute-stage patients were studied. toxic shock syndrome toxin-1 (tsst-1) and antibody to tsst-1 and enterotoxins a (sea), b (seb), and c (sec) in acute (mean, day 7) and late convalescent (mean, month 15) sera from 26 patients (12 with coronary artery aneurysms) and 22 age-matched controls were measured. only 1 of 60 supernata ... | 1995 | 7622905 |
| salmonella pyomyositis in patients with the human immunodeficiency virus. | pyomyositis is a common disease in the tropics, mostly due to staphylococcus aureus. we report two patients infected with the human immunodeficiency virus (hiv) who presented with fever and unilateral limb swelling and in whom pyomyositis was diagnosed in quadriceps and gluteus major, respectively. salmonella enteritidis was isolated in both, with recurrent episodes of muscle involvement and secondary osteomyelitis in one case. non-typhi salmonella pyomyositis may occur in hiv + patients with a ... | 1995 | 7633800 |
| differential human t cell-dependent b cell differentiation induced by staphylococcal superantigens (sag). regulatory role for sag-dependent b cell cytolysis. | microbial superantigens (sag), by virtue of their binding to tcr v beta elements and to class ii mhc molecules on accessory cells, trigger t cell proliferation in a dose-dependent fashion. in contrast, sag-induced t cell-dependent b cell differentiation occurs only at sag concentrations that are orders of magnitude lower that those required for optimal mitogenesis (low-dose sag). at optimal mitogenic doses (high-dose sag), sag-driven b cell differentiation does not ensue. in this report, we demo ... | 1995 | 7636237 |
| presence of hsp65 in bacterial extracts (om-89): a possible mediator of orally-induced tolerance? | heat shock proteins (hsp) have been implicated in rodent models of autoimmunity, particularly arthritis, and there is suggestive though inconclusive evidence that they may also play a role in human autoimmune disease. the simplest hypothesis is based on molecular mimicry due to the amino-acid sequence homology between mammalian and microbial hsp. recently om-89, an extract of several strains of escherichia coli, has shown some efficacy in the treatment of rheumatoid arthritis (ra) when taken ora ... | 1995 | 7649235 |
| b cell differentiation factor-induced human b cell maturation: stimulation of intracellular calcium release. | we have recently identified a novel human b cell differentiation factor, 446-bcdf, derived from anti-cd3-stimulated peripheral blood (pb) t cells. this novel cytokine, which may act through a pertussis toxin-sensitive gi-linked receptor, induces a 5- to 100-fold increase in immunoglobulin (ig) secretion by sac (0.001%, v/v)-activated pb b cells. coculture of b cells with 446-bcdf induces a decrease in intracellular camp which is necessary but not sufficient to drive terminal b cell differentiati ... | 1995 | 7656331 |
| detection of bacterial pyrogens on the basis of their effects on gamma interferon-mediated formation of neopterin or nitrite in cultured monocyte cell lines. | in a number of mammalian cell types, pteridine biosynthesis from guanosine 5'-triphosphate and formation of nitric oxide from l-arginine are induced by gamma interferon (ifn-gamma) and bacterial lipopolysaccharide (lps). we assessed the possibility of using such metabolic alterations for the in vitro detection of pyrogens. products from gram-negative and gram-positive bacteria and related synthetic compounds were tested for their potential to induce either of these pathways. stimulation of pteri ... | 1995 | 7664177 |
| interactions of staphylokinase with human platelets. | the interactions of recombinant staphylokinase (sakstar) with human platelets were investigated in a buffer milieu, in a human plasma milieu in vitro, and in plasma from patients with acute myocardial infarction (ami) treated with sakstar. in a buffer milieu, the activation rate of plasminogen by sakstar or streptokinase (sk) was not significantly altered by addition of platelets. specific binding of sakstar or sk to either resting or thrombin-activated platelets was very low. adp-induced or col ... | 1995 | 7667831 |
| purification to homogeneity of a novel human b cell differentiation factor. | by immunization with a partially purified form of a novel human b cell differentiation factor, 446-bcdf, we generated a series of mabs that inhibit the functional activity of this cytokine (induction of ig secretion by staphylococcus aureus cowan i strain organisms-activated b cells). two mabs, 929 and 204, were shown to be specific for 446-bcdf in that they failed to inhibit b cell differentiation in response to other known cytokines (il-2, il-6) or mitogens (pwm). more importantly, passage of ... | 1995 | 7673706 |
| immunoregulatory role of cd40 in human b cell differentiation. | cd40 is a member of a family of surface molecules with homology to the nerve growth factor receptor and is expressed on b cells. the role of cd40 in regulating human b cell differentiation and the production of specific ig isotypes was examined. in the absence of other stimuli, anti-cd40 and il-4 induced the secretion of igm, igg, and ige by purified human peripheral blood b cells. however, addition of formalinized staphylococcus aureus (sa) enhanced the response. in the presence of sa, anti-cd4 ... | 1993 | 7679424 |
| immunomodulatory effect of fosfomycin on human b-lymphocyte function. | fosfomycin (fom) is an unique antibiotic which is chemically unrelated to any other known antimicrobial agent. recent investigations have demonstrated that fom inhibits histamine release from basophils. in this study, we examined the effect of fom on human b-cell functions. fom inhibited the proliferative response of resting b cells induced by staphylococcus aureus cowan 1 in a dose-dependent manner. fom interfered with the transition from the g0 to the g1 phase of the cell cycle, leading to cel ... | 1993 | 7680847 |
| suppression of polymorphonuclear leukocyte bactericidal activity by suramin. | suramin is a polyanionic compound with potent antineoplastic properties. because polymorphonuclear leukocytes (pmns) are a crucial component of host defenses against bacteria and fungi, the effects of suramin on pmn function were studied in vitro. pmns from healthy donors were incubated with concentrations of suramin of 1 to 1,000 micrograms/ml (within and exceeding the therapeutic range) for 30 min, and pmn functional parameters were subsequently assessed. suramin had no effect on viability, ch ... | 1993 | 7681657 |
| cephalosporins and inflammatory host reactions. | the oral cephalosporins cefaclor, cefetamet, ro 40-6890 and cefpodoxime were studied with regard to their effects to modulate the chemiluminescence response, the rate of phagocytosis, bactericidal action and leukotriene formation from human granulocytes, the release of histamine from human basophils and the induction and release of tumor necrosis factor (tnf) and interleukin-6 (il-6) from a preparation of human lymphocytes, monocytes and basophils. the cephalosporins increased the histamine rele ... | 1993 | 7683137 |
| induction of cd5 antigen on human cd5- b cells by stimulation with staphylococcus aureus cowan strain i. | we have examined whether the cd5 phenotype could be induced on human b cell surfaces by the polyclonal b cell stimulator, staphylococcus aureus cowan strain i (sac). fresh tonsillar b cells were prepared by percoll density gradient from e- cells. the proportion of cd5+ b cells in the 50/60% and 60/70% interface high-density fractions varied between 1.2 and 10.2% depending on the tonsil preparations when they were placed on the in vitro culture 12-60 h prior to flow cytometric analysis. the expre ... | 1993 | 7691163 |
| g-csf enhances the immunoglobulin generation rather than the proliferation of human b lymphocytes. | the effect of granulocyte-colony stimulating factor (g-csf) on human b-cell function was studied in in vitro cultures. g-csf alone had no effect on the proliferative response of resting b cells, but it slightly enhanced the proliferative response of these cells in the presence of polyclonal b-cell mitogen, staphylococcus aureus cowan strain i (sac) at concentrations of 0.2 to 25 micrograms/ml (1.5-fold increase in the dna synthesis). in contrast, immunoglobulin (ig) secretion of activated b cell ... | 1993 | 7691653 |