| induction of monocytic suppression after stimulation of peripheral human mononuclear cells with staphylococcal protein a and staphylococcus aureus. | staphylococcal protein-a (spa) and staphylococcus aureus are known to be polyclonal human b-cell activators. it was noted that they induced plaque-forming-cell (pfc) responses lower than those induced by pokeweed mitogen (pwm) and the possibility of early triggering of a suppressor cell was investigated in the present series of experiments. peripheral mononuclear cells (mnc) were passed through sephadex g-10 columns to eliminate monocytes. the pfc responses to spa and s. aureus were thereby incr ... | 1983 | 6342815 |
| storage of protein a-conjugated sheep erythrocytes for use in plaque-forming cell (pfc) assays. | sheep red blood cells conjugated with protein a from staphylococcus aureus were frozen in liquid nitrogen. after thawing these cells were used as indicator cells in a plaque-forming cell assay visualizing human b lymphocytes secreting igg, igm or iga. the thawed erythrocytes performed as well in the assay as erythrocytes freshly conjugated with protein a. recovery of conjugated erythrocytes after freezing and thawing averaged 66%. by the method described indicator cells for a number of pfc assay ... | 1983 | 6343496 |
| [nosocomial dyspepsia in newborn and young infants. a 15-month prospective study with continuous rotavirus surveillance]. | a 15 months prospective study of gastroenteritis in hospitalized newborns and infants (n = 201) was combined with a rotavirus infection surveillance. stool specimens were investigated weekly (cft). from patients with gastroenteritis (n = 320) an infectious agent could be detected on average in 41% of each patient group: rotavirus n = 54, staph. aureus n = 22, toxin producing e. coli n = 4, other bacteria n = 25, candida albicans n = 17, echovirus 11 n = 1. with certain exceptions, the pathogens ... | 1983 | 6353207 |
| bacterial adherence to fibronectin and endothelial cells: a possible mechanism for bacterial tissue tropism. | in the pathophysiology of endocarditis, bacteria must initially adhere to the endothelial surface components of the cardiac valve before invasion and colonization. the attachment of bacteria to endothelial cell surfaces is dependent on surface characteristics of both the bacteria and the endothelial cell. fibronectin, a glycoprotein produced by endothelial cells, binds to some bacteria but not all. this report examines bacterial adherence to human endothelial cells and to fibronectin-coated surf ... | 1984 | 6361186 |
| multiple isoelectric and molecular weight variants of choline acetyltransferase. artifact or real? | the existence of isoelectric variants of bovine brain and human placental choline acetyltransferase was confirmed by chromatofocusing. the identification of molecular weight variants (bovine brain, mr = 68,000 and 63,000; human placental, mr = 66,000 and 64,000) was also demonstrated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by "western blotting" and immunochemical visualization of choline acetyltransferase with monoclonal antibodies. no correlation between the iso ... | 1984 | 6363403 |
| lipolytic and proteolytic properties of staphylococci. | lipolytic and proteolytic activities were estimated in coagulase-positive and coagulase-negative strains of human origin. several different substrates were used for determination of these activities. staphylococcus aureus strains were very frequently lipolytic (95%) and proteolytic (87.5%). among coagulase-negative strains, 85.4% were proteolytic and 10.25% exhibited lipolytic activity. | 1983 | 6372327 |
| [stimulation of lymphokine formation as an index of reactivity to bacterial antigens]. | in the cultures of lymphocytes from healthy donors, activated by staphylococcus aureus, streptococcus pyogenes and enterococcal antigens (allergens) the formation of lymphokines stimulating the oxygen-dependent metabolism of human neutrophils was studied. the preparations yielded positive reactions in a wide range of concentrations (2-60 micrograms/ml); in experiments with concentrations of 2 micrograms/ml these reactions were observed, respectively, in 71.5%, 40.0% and 47.7% of the donors. indi ... | 1984 | 6377781 |
| enhanced bacterial phagocytosis by peripheral blood monocytes in rheumatoid arthritis. | to assess the functional state of peripheral blood monocytes in rheumatoid arthritis, we have measured phagocytosis of staphylococcus aureus and proteus mirabilis in 48 patients and 28 controls. using radiolabelled bacteria preopsonised in normal human serum we have demonstrated significantly enhanced uptake of both organisms by patients' monocytes: (median % uptake staph. aureus: patients = 35.8; controls = 19.3; p less than 0.001. median % uptake p. mirabilis: patients = 32.3; controls = 19.8; ... | 1984 | 6378106 |
| clinical trials with staphylococcus aureus and protein a in the treatment of malignant disease. | perfusion of plasma from tumor-bearing animals over staphylococcus aureus with membrane-bound protein a has resulted in significant tumor shrinkage. similar therapy has now been given to 16 patients by three methods. no tumor responses have been observed. five patients were treated with perfusion over fixed and killed s. aureus cowan i. cardiovascular and respiratory toxicity was excessive and appeared to be related to volume and rate of plasma infused. eight patients were treated with perfusion ... | 1984 | 6379115 |
| ability of various oral bacteria to bind human plasma fibronectin. | the present study describes the ability of various oral bacteria to bind human plasma fibronectin (pfn). avid binding of 125i-pfn was found for streptococcus mutans (serotypes a to h), streptococcus sanguis, group a streptococcus pyogenes and staphylococcus aureus, while other gram-positive bacterial species tested demonstrated only weak or negligible pfn binding ability. two gram-negative bacterial species, bacteroides gingivalis and escherichia coli, did not significantly bind pfn. 125i-pfn bi ... | 1984 | 6390098 |
| protease activity in fibromyoma and normal human myometrium. | the potential involvement of intracellular proteases in the pathophysiology of fibromyoma was studied by assay of activity of two proteolytic enzymes in fibromyoma and adjacent normal myometrium. protease i, a soluble acid protease and protease ii, a particulate protease optimally active at the neutral ph range are present in fibromyoma as well as in normal myometrial tissue. no consistent difference was observed between the specific activities of these enzymes in the fibromyoma and the surround ... | 1984 | 6396176 |
| peripherin, a new member of the intermediate filament protein family. | peripherin, a triton-insoluble protein, whose distribution was found to be restricted to neurons in the rodent and human peripheral nervous system, was characterized by its electrophoretic features (isoelectric point: 5.6; molecular weight: 56,000 daltons) and by its peptidic map after limited proteolysis. comparative peptide analysis of the 70,000-dalton subunit of neurofilaments (70k nfp), vimentin and peripherin, was performed by two different methods; limited proteolysis with staphylococcus ... | 1983 | 6399022 |
| partial amino acid sequence of a rabbit immunoglobulin light chain of allotype b5. | the amino acid sequence of a rabbit immunoglobulin light chain of allotype b5 has been nearly completed. a comparison of its structure with that of light chains of allotypes b4, b6, and b9 confirms that the constant regions of these various kappa chains differ by 20-35%. the substitutions are clustered in parts of the second half of the chain, and the b5 form bears more resemblance to the b6 chain than to any other, in good agreement with previous serological data. the analysis of the variable r ... | 1983 | 6404296 |
| [molecular profile of mitogen-active metabolites of staphylococcus aureus]. | a fraction with a molecular weight of 16000-24000 was isolated from 5-day s. aureus broth culture grown in the dialysate of martin-hottinger broth. at a concentration of 2-15 micrograms/ml this fraction induced the nonspecific blast transformation of human blood lymphocytes with the stimulation index 99.6 +/- 15.2 (72-hour cultures). the mitogenic fraction was immunochemically inert and unrelated to the main part of biologically active components, resistant to heating at 100 degrees c for 10 min ... | 1983 | 6405563 |
| in vitro inhibition of lymphocyte proliferation by pseudomonas aeruginosa phenazine pigments. | human lymphocyte proliferation is inhibited in vitro in the presence of killed pseudomonas aeruginosa or cell-free p. aeruginosa culture supernatants. a comparison of culture supernatants obtained under similar conditions from staphylococcus aureus, escherichia coli, p. aeruginosa, and pseudomonas cepacia strains demonstrated that all p. aeruginosa supernatants were strongly inhibitory, whereas supernatants from other bacteria were mildly inhibitory or not inhibitory at all. these p. aeruginosa ... | 1983 | 6408002 |
| susceptibility of brain to aerobic, anaerobic, and fungal organisms. | the utility of an experimental animal model is dependent on its ability to simulate the actual clinical situation. with a stereotaxic injection procedure, the susceptibility of rat brain to the spectrum of organisms commonly associated with human brain abscess was determined. two strains of escherichia coli were more infective than pseudomonas aeruginosa, staphylococcus aureus, and streptococcus pyogenes. even between the e. coli strains it was possible to document significant differences in deg ... | 1983 | 6409808 |
| cefamandole therapy in hand infections. | hand lacerations, especially when inadequately treated, may result in infections caused by aerobic or anaerobic bacteria. anaerobic infections most commonly result from human bite injuries in which there is contact between hand and mouth. the search continues for an ideal antibiotic to employ when anaerobic organisms are suspected. in this study cefamandole, a new cephalosporin antibiotic, was employed whenever anaerobic hand infections were suspected following trauma. in each patient quantitati ... | 1983 | 6415153 |
| quantitative adherence of bacteria to human corneal epithelial cells. | an in vitro assay was used to quantitatively determine the ability of a variety of bacteria to adhere to human corneal epithelial cells. staphylococcus aureus, streptococcus pneumoniae, and pseudomonas aeruginosa were found to adhere to corneal epithelium significantly better than other species that were tested. however, there was considerable variability in the adherence of individual strains of these bacterial species. the results of this study support the hypothesis that the frequency with wh ... | 1984 | 6433864 |
| human interleukin-2 promotes proliferation of activated b cells via surface receptors similar to those of activated t cells. | human interleukin-2 (il-2) is a glycoprotein of relative molecular mass (mr) 15,000, which is released by t lymphocytes on stimulation with antigen or mitogen and functions as a t-cell growth factor (tcgf) by inducing proliferation of activated t cells. it is generally accepted that resting or activated b cells do not respond directly to il-2 but require for their proliferation other t-cell-derived lymphokines usually referred to as b-cell growth factors (bcgfs). recently, however, a monoclonal ... | 1984 | 6438535 |
| [bathing caps and germ shedding of human scalp hair]. | with three groups of 30 test subjects each, having short, medium-long and long hair, as well as with a model test using a woman's wig it was possible to show that by wearing bathing caps the transfer of germs from the scalp hairs to the swimming-pool water can be reduced considerably, either by the isolation of the hairs from water when snugly fitting water-tight bathing caps are used, or by a reduction of the germ-carrying hair surface rinsed by the water, which is always the case, even when us ... | 1984 | 6441382 |
| [determination of macroglobulin antibodies by the methods of treating the blood sera with 2-mercaptoethanol and staphylococcus aureus protein a]. | | 1983 | 6443862 |
| resistance transfer in mixed cultures of staphylococcus aureus. | transfer of resistance markers was regularly observed in mixed cultures of pairs of constructed strains of staphylococcus aureus. this transfer was shown to be due to general transduction and required a lysogenic donor strain, carrying a transducing phage of serotype b and a transducible plasmid, and an acceptor strain without either strong restriction or an incompatible plasmid. transfer was sometimes bidirectional when both strains carried a transducing phage. unidirectional transfer occurred ... | 1981 | 6450835 |
| rarity of gene transfer between animal and human isolates of staphylococcus aureus in vitro. | antibiotic-resistant staphylococcus aureus strains of animal origin were studied for transfer of resistance to human strains in vitro, with special reference to tylosin, an antibiotic which is used only in animals. resistance to tylosin in animal and human strains was part of a constitutive resistance to all macrolide antibiotics. resistance to tylosin could not be transferred from animal to human strains in vitro. transfer of other resistances occurred between few strains and at low frequency. ... | 1980 | 6453205 |
| anomalous effect of serum on the antimicrobial activity of cefoperazone. | this study investigated the effect of added serum on cefoperazone activity in vitro. due to protein-binding, an 8-fold difference would be expected between minimum inhibitory concentrations in broth and in broth plus 90% serum. using supplemented mueller-hinton broth (smh) and smh plus 90% heat-inactivated human serum there were differences of 3.36 +/- 0.92 for staphylococcus aureus strain atcc1 25923, 1.39 +/- 0.47 for escherichia coli strain atcc 25922 and 1.43 +/- 0.39 for pseudomonas aerugin ... | 1981 | 6456892 |
| studies on possible cross transmission of mercuric chloride resistant staphylococcus aureus between dogs and kennel attendants. | ten s. aureus strains were each introduced into the nostrils of each of 10 dogs. the dogs retained the strains of s. aureus for the period of the experiment (eight weeks). five of them with human strains of s. aureus, and the two control dogs acquired strains of canine origin during the period, but no human strain was detected in the nostrils of those into which canine strains were introduced. one of the dogs (dog 4) died during the period of the experiment and both human and canine s. aureus we ... | 1981 | 6460716 |
| enhancement of human polymorphonuclear leucocyte motility by erythromycin in vitro and in vivo. | the effects of erythromycin on the migration, phagocytosis and antimicrobial activity of human polymorphonuclear leucocytes (pmnls) were investigated in vitro. therapeutic concentrations of erythromycin potentiated pmnl staphylocidal activity without affecting phagocytosis. pmnl random motility and migration to leuco-attractant endotoxin-activated serum (eas) were significantly enhanced by erythromycin at concentrations of greater than 5 x 10(-5)m (16,5 micrograms/ml). to assess the possible in ... | 1984 | 6463793 |
| staphylococcal toxic shock toxin specifically binds to cultured human epithelial cells and is rapidly internalized. | staphylococcal toxic shock syndrome toxin (tst) was labeled with 125i under mild conditions without apparent destruction of the molecule. [125i]tst bound specifically to human epithelial (chang) cells in culture; the binding was inhibited by a 100-fold excess of unlabeled toxin. scatchard analysis of the binding data indicated about 10(4) receptor sites per cell and a dissociation constant (kd) of 4 x 10(-9) m. when cells pretreated with tst at 4 degrees c were swiftly transferred to 37 degrees ... | 1984 | 6469347 |
| rifampicin in the treatment of osteoarticular infections due to staphylococci. | rifampicin is a suitable drug for treating staphylococcal bone and articular infections, because high levels are obtained in the human tissues. the best oral dose is 600 mg 12 hourly. the serum levels range from 10 to 15 mg/l at the peak concentration and from 0.2 to 0.6 mg/l 8 h after oral administration of 600 mg. the ratio for cancellous bone/serum is 0.41 at 3 h and 0.39 at 12 h, and for cortical bone/serum is 0.20 at 3 h after a dose of 600 mg. in every case, tissue levels paralleled serum ... | 1984 | 6469888 |
| flow cytometric quantitation of oxidative product formation by polymorphonuclear leukocytes during phagocytosis. | stimulation of the oxidative metabolic burst of human polymorphonuclear leukocytes (pmnl) may occur by an all-or-none trigger mechanism or by a graded response to increasing stimulation of an individual cell. if the proposed all-or-none mechanism occurred during phagocytosis, a pmnl would expend all of its metabolic potential at once, yet pmnl can proceed to ingest multiple organisms. this study employed dual laser flow cytometry to correlate the number of cell-associated organisms with oxidativ ... | 1984 | 6491287 |
| subclass distribution of human anti-staphylococcus aureus alpha toxin antibodies: suggestion of an igg1, iga1, igg4 switch pattern. | the subclass distribution of antibodies against alpha toxin from staphylococcus aureus in man was investigated. antibodies were restricted to igg1, iga1, and igg4 and appeared to develop sequentially. these data suggest a distinct pattern of class and subclass switches in response to protein antigens. however, studies in immunodeficient (class- or subclass-deficient) donors show that the proposed patterns is not obligatory. | 1984 | 6494808 |
| correlation between toxin binding and hemolytic activity in membrane damage by staphylococcal alpha-toxin. | the binding of staphylococcus aureus alpha-toxin to rabbit and human erythrocytes was studied by hemolytic assays and sodium dodecyl sulfate-polyacrylamide gel electrophoresis immunoblotting. hemolytic assays showed that toxin binding to 10% cell suspensions at neutral ph was very ineffective in the concentration range 3 x 10(-8) to 3 x 10(-7) m (1 to 10 micrograms/ml), and less than 5% of added toxin became cell bound. however, binding was augmented as toxin levels were raised, abruptly increas ... | 1984 | 6500692 |
| opsonic activity of immunoglobulin prepared for intravenous use. | the opsonic activity of two immunoglobulin preparations modified for intravenous infusion was tested against streptococcus pneumoniae types 3, 7f, and 14 and two strains of staphylococcus aureus by polymorphonuclear leukocyte uptake of 3h-thymidine-labeled bacteria. reduced and alkylated immunoglobulin (chem-igg) and immunoglobulin prepared by chromatography with diethylaminoethyl-sephadex (deae-igg) were evaluated with and without complement and compared with the opsonic activity of immune seru ... | 1984 | 6502004 |
| substitution of anti-human globulin by protein a-bearing staphylococci in the detection of brucella antibodies. | a coagglutination test using protein a-bearing staphylococci has been developed for the detection of brucella antibodies. comparing the results of a random sample of 57 sera collected from malta fever patients, suggestive titers of 1: greater than or equal to 160 were found in 8 sera (14%) with the standard agglutination test, in 22 sera (39%) with the coombs test, and in 23 sera (40%) with the coagglutination test. the titers in the coombs test and the coagglutination test coincided in 54 (95%) ... | 1984 | 6513866 |
| modulation of phagocytosis and intracellular bactericidal activity of polymorphonuclear and mononuclear cells by cationic proteins from human granulocytes: alternative pathway of phagocytic enhancement. | cationic lysosomal proteins from human polymorphonuclears (pmn) were isolated by column chromatography and divided into five fractions. on acrylamide gel electrophoresis, fraction i had four bands slower than lysozyme (lzm) mobility; fraction ii had five or six bands slower than lzm; fraction iii had at least seven bands slower and two bands faster than lzm; fraction iv contained lzm, two bands faster and a few faint bands slower than lzm; fraction v was composed of almost pure lzm. partial char ... | 1984 | 6519776 |
| [comparative bacteriological and chemical analysis of kidney calculi. apropos of 135 cases]. | the formation of some urinary tract stones (struvite stones) is known to be related to infection by urease-possessing microorganisms, such as proteus sp. and some other bacteria. ureaplasma urealyticum, a genital mycoplasma, contains also urease and is predominantly located in the urogenital tract. its significance in the production of human urinary stones has not yet been elucidated. in this study, 135 human calculi obtained by surgery were analysed chemically and were cultured for the presence ... | 1984 | 6531061 |
| effect of human igg and fibrinogen on staphylococcus aureus intraperitoneal infection in mice. | human serum and plasma have been demonstrated to enhance mortality in staphylococcus aureus intraperitoneal infection in mice. two different mechanisms seem to be involved. the effect of serum could be removed by adsorption to s. aureus protein a coupled to sepharose 4 b and could be reconstituted by the addition of human igg to igg-depleted serum. plasma diluted 1/10 in combination with purified fibrinogen also enhanced mouse mortality. both effects could be demonstrated, when a coagulase-free ... | 1984 | 6532110 |
| laboratory evaluation of the serum dilution test in serious staphylococcal infection. | four test media were studied to determine performance characteristics of serum dilution tests used to monitor antimicrobial therapy during serious staphylococcus aureus infection being treated with highly protein-bound antibiotics. serum inhibitory titers and serum bactericidal titers obtained with mueller-hinton broth supplemented with calcium and magnesium were 3- to 16-fold higher than titers obtained with whole human serum buffered with n-2-hydroxyethylpiperazine-n'-2-ethanesulfonic acid (he ... | 1983 | 6554031 |
| gentamicin- and methicillin-resistant, clinical isolates of staphylococcus aureus: comparative in vitro and in vivo efficacy of alternative antimicrobial drugs. | six representative clinical isolates of gentamicin- and methicillin-resistant (grmr) staphylococcus aureus, constituting phage groups ii and iii, were susceptible only to amikacin, cefamandole, clindamycin, fosfomycin, fusidic acid, netilmicin, nitrofurantoin, trimethoprim-sulfamethoxazole, and vancomycin (bauer-kirby test). with few exceptions, the minimal bactericidal concentrations of the beta-lactam and aminoglycoside antibiotics tested, fusidic acid, and irregularly those of vancomycin, but ... | 1984 | 6559664 |
| identification, clinical distribution, and susceptibility to methicillin and 18 additional antibiotics of clinical staphylococcus isolates: nationwide investigation in italy. | a multicentric study of clinical staphylococcus isolates was performed by seven operative units working in different areas of italy. over a 6-month period, a total of 3,226 staphylococci, isolated from in- and outpatients, were identified and tested for antimicrobial susceptibility by a protocol agreed upon by all units. on the basis of their bacteriolytic-activity patterns and other conventional tests, the isolates were identified by lyogroups , which closely correlate with human staphylococcus ... | 1984 | 6565709 |
| methicillin-resistant staphylococcus epidermidis blepharitis. | blepharoconjunctivitis caused by methicillin-resistant strains of staphylococcus epidermidis occurred in two hospitalized patients, a 74-year-old man and a 19-year-old man. both strains were resistant to multiple courses of topical antibiotic therapy. successful treatment in both cases depended upon antibiotic sensitivity testing. these methicillin-resistant strains of s. epidermidis, like methicillin-resistant strains of staphylococcus aureus, demonstrated almost complete cross-resistance to ce ... | 1984 | 6568091 |
| recognition of staphylococcus aureus by human phagocytes. signals and disguises of the bacterial surface. | phagocytic cells provide the host its major defense against invasive staphylococcus aureus, and the staphylococcal surface, by its influence on phagocyte recognition, is a primary determinant of the function of these cells. the peptidoglycan component of the cell wall plays a key role in both opsonic and chemotactic recognition, mediated by igg, c3b, and c5a, respectively. while cell wall protein a inhibits opsonic recognition by polymorphonuclear leukocytes, it promotes an opsonin-independent m ... | 1983 | 6589763 |
| amino acid sequence of human beta-factor xiia. | human factor xii was activated by limited proteolysis with trypsin, and the resulting beta-factor xiia (mr = 30,000) was isolated by deae-sephacel column chromatography. the complete amino acid sequence of beta-factor xiia was then determined on peptides produced by enzymatic digestion with either trypsin, chymotrypsin, or staphylococcus aureus v8 protease and by chemical cleavage at methionyl and tryptophyl bonds. beta-factor xiia is a glycoprotein composed of a heavy chain (243 amino acid resi ... | 1983 | 6604055 |
| monocyte-independent stimulation of human b lymphocytes by phorbol myristate acetate. | phorbol myristate acetate (pma) induces a low level of proliferation in purified human b lymphocytes when added in nanomolar concentrations to the culture medium. much higher levels of thymidine incorporation, however, are obtained in the presence of other b cell stimuli such as anti-igm antibodies or staphylococcus aureus cowan strain 1 (sac). the peak activity of pma was observed on day 3 of b cell cultures containing either anti-igm or sac. the rigorous depletion of monocytes as well as add-b ... | 1983 | 6605383 |
| comparison of in vitro activity of fce 22101, a new penem, with those of other beta-lactam antibiotics. | the in vitro activity of fce 22101, a new semisynthetic penem derivative, was compared with that of ceftriaxone, moxalactam, imipenem (formerly imipemide, n-formimidoyl thienamycin, or mk 0787), cefuroxime, ceftazidime, and other beta-lactams, when appropriate, against 472 recent isolates and known beta-lactam-resistant strains. the minimum inhibitory concentrations of fce 22101 against 90% of the members of the family enterobacteriaceae, haemophilus influenzae, staphylococcus aureus, lancefield ... | 1983 | 6607032 |
| changes in deoxyadenosine production during human lymphocyte mitogenesis. | immunodeficient children who lack the purine metabolic enzyme adenosine deaminase have markedly elevated plasma concentrations of 2'-deoxyadenosine and adenosine. however, little information exists concerning the magnitude of endogenous 2'-deoxyadenosine and adenosine synthesis by normal human hematopoietic cells. in the present experiments, we have used the sensitive technique of high performance liquid chromatography to quantitate changes in 2'-deoxyadenosine and adenosine production during hu ... | 1984 | 6607841 |
| [characteristics of staphylococci isolated from normal human skin]. | | 1983 | 6608194 |
| perfusion of canine serum over staphylococcus aureus cowan 1: evidence for release of protein a and changes in specific antibody activity. | sera from three normal dogs were assessed for levels of clq binding igg and complement consumption after perfusion over staphylococcus aureus cowan i (sac). increased levels of clq binding igg were detected after perfusion of sera over sac and were associated with complement consumption. canine antiserum to human erythrocytes were also perfused over sac and assessed for clq binding igg and hemolytic activity. increased levels of clq binding igg in post-perfusion samples were detected which were ... | 1983 | 6609117 |
| in vitro activity of fludalanine combined with pentizidone compared with those of other agents. | the in vitro activity of fludalanine ( mk641 ) combined with pentizidone ( mk642 ) so as to give a fludalanine /d-cycloserine ratio of 1:1 was compared with the activities of ampicillin, ticarcillin, cefuroxime, ceftazidime, and trimethoprim against 452 recent isolates and known beta-lactam- and trimethoprim-resistant strains. in addition, the in vitro activity of fludalanine - pentizidone on four different media, including a defined medium ( dfn -2), was studied. the mic of fludalanine - pentiz ... | 1984 | 6610389 |
| effect of beta-lactam antibiotics on migration and bactericidal activity of human phagocytes. | the effect of beta-lactam antibiotics upon some functions of human phagocytes was examined. penicillins (carbenicillin and piperacillin), thienamycin, and cephalosporins (cefotetan, ceftazidime, and moxalactam) had no effect on either the random or directional migration or on the bactericidal activity of neutrophils and monocytes against staphylococcus aureus. on the contrary, cefoperazone at therapeutic levels was shown to inhibit neutrophil chemotaxis. | 1983 | 6614895 |
| microbiological investigations on cryopreserved human semen. | primary mycological contamination was studied in 565 random human ejaculates. after sperm preservation at low temperature, secondary mycological contamination of 55 of the ejaculates was investigated. 25 ejaculates were cryopreserved with and without antibiotics and examined bacteriologically before and after different phases of cryopreservation. furthermore, semen samples of several donors were inoculated with suspensions of different concentrations of yeasts and with the test bacteria escheric ... | 1983 | 6618690 |
| ozone killing action against bacterial and fungal species; microbiological testing of a domestic ozone generator. | the action of ozone generated from a small domestic device was examined with a view to using it in clinical isolation units accommodating immunosuppressed patients. over a six-hour period in an average size room the device did not generate sufficient ozone to suppress bacterial and fungal growth. a useful bactericidal action, against a variety of human pathogens was achieved with ozone concentrations between 0.3 to 0.9 ppm. bactericidal ozone concentrations are close to the limit permitted for h ... | 1983 | 6619309 |
| effect of staphylococcus aureus enterotoxin f on human neutrophil oxidative metabolism. | epidemiologic and animal challenge studies have suggested that sef may play a critical role in toxic-shock syndrome [1]. however, the pathogenic mechanism of sef activity is not known. one means by which this toxin could elicit the widespread organ dysfunction observed in toxic-shock syndrome would be by directly promoting pmn production of toxic oxygen species and the resultant secondary endothelial cell damage [2]. to evaluate this possibility, we assayed the effect of sef on pmn oxidative met ... | 1983 | 6631066 |
| [quantitative characteristics of milk microflora in the diagnosis of puerperal mastitis]. | | 1983 | 6638368 |
| clindamycin enhances opsonization of staphylococcus aureus. | staphylococcus aureus 502a was grown in the presence of one-third of the minimal inhibitory concentration of clindamycin. phagocytosis of the antibiotic-treated bacteria by human polymorphonuclear leukocytes was significantly enhanced, compared with that of the untreated control (p less than 0.001). study of opsonization kinetics by a chemiluminescence assay demonstrated that clindamycin-treated staphylococci were opsonized more rapidly than control bacteria and that the serum concentration requ ... | 1983 | 6638997 |
| examination of neutrophil function in a rat model of decreased host resistance following burn trauma. | the high incidence of serious opportunistic infection following human burn injury has been well documented. investigations of the mechanisms of this acquired susceptibility have demonstrated several defects in phagocytic defenses. an established rat burn infection model was modified for study of neutrophil function in animals with a 60% burn injury. these 350-g rats received a 35-ml saline resuscitation, and when not further stressed, 80% of the animals survived to healing. burned animals were f ... | 1983 | 6658285 |
| [reactivity to staphylococcus aureus peptidoglycan in a neutrophil phagocytosis system]. | the mechanisms of the formation of reactivity in the system "s. aureus peptidoglycan--serum--neutrophils" were studied. the direct interaction of peptidoglycans with neutrophils isolated from human blood was not accompanied by their stimulation, evaluated by an increase in the reduction of nitro blue tetrazolium. antibodies to peptidoglycan determined the final result of the opsonic activity of normal serum: the igg fraction isolated from commercial immunoglobulin was active at a concentration o ... | 1983 | 6666448 |
| latex agglutination test to identify coagulase, clumping factor and protein a produced by staphylococci. | since a rapid latex agglutination method for identifying staphylococcus aureus has recently been described, we attempted to identify which factors in staphylococci produce a positive reaction so as to indicate the possible applications and limitations of the test. latex particles prepared with human plasma (latex-plasma), immune serum globulin (isg) (latex-isg) or bovine fibrinogen (latex-fibrinogen) were evaluated. purified protein a or staphylococcal colonies possessing protein a caused latex- ... | 1983 | 6671360 |
| monoclonal antibody to human eosinophils recognizing 95 kd surface membrane antigen. | mice were immunized with purified eosinophils obtained from patients with the idiopathic hypereosinophilic syndrome. a hybridoma initially producing an igm antibody which switched to an igg1 antibody was selected for cloning and further testing. this igg1 antibody reacted with human eosinophils, granulocytes, monocytes and large granular lymphocytes, but did not react with t lymphocytes, b lymphocytes, platelets, erythrocytes, or a panel of human leukemia cells and cell lines. bone marrow analys ... | 1983 | 6678804 |
| effect of iron (iii) in the presence of various ligands on the phagocytic and metabolic activity of human polymorphonuclear leukocytes. | fecl3 or fe(iii) that attached to chelating ligands such as citrate or nitrilotriacetic acid (nta) at a molar ratio of 1:1 had a toxic effect on pmn. uptake of radiolabeled staphylococcus aureus by pmn, preincubated for 2 hr at 37 degrees c in a medium containing fe(iii)-citrate or fe(iii)-nta, was significantly lower than that of control pmn preincubated without excess iron (p less than 0.002). however, at a 1:2 molar ratio of fe(iii) to citrate or nta, the iron was not toxic. in contrast, the ... | 1984 | 6690620 |
| asbestos-induced chemiluminescence response of human polymorphonuclear leukocytes. | products of the oxidative metabolism of polymorphonuclear leukocytes (pmn), namely hydrogen peroxide and superoxide, may play a role in tissue injury at sites of inflammation. oxidant production by pmn was measured by the luminol-enhanced chemiluminescence (cl) of cells exposed in vitro to asbestos fibers. human peripheral blood pmn were incubated with 1-1000 micrograms/ml of uicc chrysotile b asbestos fibers opsonized with either normal or heat-inactivated human serum. cl was measured over 210 ... | 1984 | 6692804 |
| [brain abscess--a possible complication of cerebral infarction?]. | a 68-year-old man with arterial hypertension and diabetes mellitus suffered a stroke with hemiparesis. the ischemic infarction was demonstrated by computer assisted tomography and angiography. at the site of the original infarction a large brain abscess developed several months later and caused the patient's death. a secondary hematogenous infection of the necrotic area (in this case staphylococcus aureus, probably from bronchiectasis) seems likely. other cited case reports show that after cereb ... | 1984 | 6701486 |
| staphylococcus aureus and human platelets cause pulmonary hypertension and thromboxane generation in isolated saline-perfused rabbit lungs. | the potential contributions of bacterial-platelet interactions to the development of acute edematous lung injury, such as that seen in the adult respiratory distress syndrome (ards), remains unknown. we found that the addition of staphylococcus aureus, 502a to isolated rabbit lungs perfused with saline, and human platelets rapidly decreased the number of circulating platelets, increased pulmonary artery perfusion pressures, and generated thromboxane b2, the stable derivative of thromboxane a2. i ... | 1984 | 6703490 |
| advantages of sequential polyethylene glycol (peg) precipitations to isolate and analyze circulating immune complexes. | the present report describes a new approach utilizing sequential polyethylene glycol 6000 (peg) precipitations to isolate and analyze circulating immune complexes (ic). two experimental systems were studied. first, ic were formed by adding labeled or unlabeled bovine serum albumin (bsa) to anti-bsa antiserum. sequential precipitations with peg concentrations ranging from 2 to 6.2 indicated that immunoglobulin (ig) molecules were precipitated in all experimental and control sera tested. in contra ... | 1984 | 6706381 |
| characterization of partially purified group b streptococcal clumping factor. | using a strain of group b streptococci, characteristics of the streptococcal clumping factor was investigated. in contrast to that of staphylococcus, the streptococcal clumping factor reacted with plasmas from guinea-pig and sheep although staphylococcal clumping factor did not react with them. in addition, streptococcal clumping factor was resistant to heat, sonication, and chemical reagents; it was also degradable by proteolytic enzyme and galactose oxidase. hydrochloric acid extract of the wh ... | 1982 | 6753418 |
| studies on bacterial hypersensitivity in man. interrelationship between skin reactions to bacterial peptidoglycan and serum peptidoglycan antibody titers. | the nature and extent of bacterially induced allergies are difficult to define. since peptidoglycan, the main component of the cell wall of almost all bacteria, has been available in a highly purified, chemically and immunologically well-defined form, investigation of the allergological significance of this cell component is feasible. intracutaneous tests were carried out on 181 test subjects with five different peptidoglycan (pg) preparations from staphylococcus aureus, staphylococcus epidermid ... | 1982 | 6753628 |
| do well-to-do and repeatedly rejecting renal allografts express the transplantation antigens similarly on their surface? | we have performed fine-needle aspiration biopsies to well-to-do and repeatedly rejecting renal allografts. after biopsy the aspirated cells were treated either (a) with monospecific rabbit antisera to hla-abc, -dr or beta 2m, or (b) with ordinary anti-hla-a and b alloantisera. after wash, the cell-bound antibody was chased with staphylococcus aureus cowan i bacteria and the antibody-binding cell type(s) was analyzed from stained cytocentrifuged cell smears. in normal, non-transplanted human kid ... | 1981 | 6755686 |
| short-time low-temperature pasteurisation of human milk. | using the oxford human milk pasteuriser human milk was exposed to lower temperature and shorter time periods than employed in classical holder pasteurisation. heating human milk at 62.5 degrees c for 5 min completely destroyed escherichia coli, staphylococcus aureus, and group b beta-haemolytic streptococci inoculated into the samples. heating at 56.0 degrees c for 15 min destroyed over 99% of the inoculated organisms. the mean percentage remaining activity of certain antimicrobial proteins afte ... | 1982 | 6756884 |
| molecular organization of human l' and l pyruvate kinases. | extremities, peptide maps and phosphorylatable site localization of human erythrocyte l' and liver l pyruvate kinases (ec 2.7.1.40) were investigated. l' and l subunits seemed to have similar, blocked nh2 termini and differ in their sensitivity to carboxypeptidase a, that is to say in their c-terminal ends. after digestion by staphylococcus aureus v8 protease, the phosphorylated sites of both l' and l subunits were located on those peptides which were different in l' and l, that is to say on the ... | 1982 | 6758852 |
| bacteriolytic activity of human gingival exudate. | we investigated the bacteriolytic activity of gingival crevicular fluid (cf) on 14c-labeled streptococcus faecalis, streptococcus mutans, staphylococcus aureus, and on whole dental plaque. cf was collected from 100 healthy donors pooled and centrifuged at 200 g. cf supernate and a frozen and thawed extract of the pellet were interacted with the different bacterial strains, while streptococcus faecalis and staphylococcus aureus released 60% and 75% of the radioactive label, only 38% of it was sol ... | 1980 | 6771214 |
| effects of human neutrophils and monocytes on nocardia asteroides: failure of killing despite occurrence of the oxidative metabolic burst. | to study mechanisms of resistance to nocardia asteroides in vitro, suspensions of human neutrophils and monocytes were challenged with n. asteroides in the presence of autologous serum. at 4 hr, numbers of viable n. asteroides incubated with neutrophils and monocytes averaged only 10% and 21% less, respectively, than numbers of n. asteroides incubated without phagocytes. in contrast, a mean of > 90% of listeria monocytogenes and staphylococcus aureus incubated with neutrophils and monocytes was ... | 1980 | 6777435 |
| postantibiotic suppression of bacterial growth. | persistent suppression of bacterial growth following exposure of both gram-positive and gram-negative bacteria to numerous antimicrobial agents was studied. the persistent, or postantibiotic, effect was quantitated by periodic counts of colony-forming units after removal of the drug by washing, dilution, or inactivation with penicillinase. although a postantibiotic effect was observed with all drugs studied, there were marked differences among drugs in their postantibiotic effects on certain org ... | 1981 | 6784225 |
| human seminal plasma inhibition of antibody complement-mediated killing and opsonization of neisseria gonorrhoeae and other gram-negative organisms. | seminal plasma diluted 1:5-1:1,000 gave marked inhibition of serum antibody complement-mediated bactericidal and opsonic effects against neisseria gonorrhoeae and other gram-negative organisms. serum that was bactericidal at a dilution of 1:5,120 was not bactericidal at a dilution of 1:10 when seminal plasma was added. bactericidal action of immune human or rabbit sera, or purified immunoglobulin (ig)g or igm plus complement for six strains of n. gonorrhoeae, serogroups a, b, c, and y of neisser ... | 1981 | 6785314 |
| expression of biological effector functions by immunoglobulin g molecules lacking the hinge region. | several biological effector functions mediated by sites on the fc region of human igg1 have been studied in two variant igg1 kappa monoclonal proteins (dob and lec) which contain deletions corresponding to the entire hinge region of the heavy chains. neither dob nor lec protein in aggregated form was able to activate the classical complement pathway, and this was shown to be due to an inability to bind the first component of complement (c1). by rosette inhibition assays, dob and lec proteins wer ... | 1981 | 6787591 |
| [causes of the antibacterial action of amniotic fluid (author's transl)]. | the activity of e. coli, group b streptococci, strept. faecalis, stap. epidermidis and staph. aureus in human amniotic fluid was studied. in comparison to standard i broth an inhibited growth of test organisms was observed. the bacteriostatic effect of amniotic fluid was most significant with staph. epidermidis. we further investigated the causes for this bacterial growth inhibition with staph. epidermidis. we showed that it is independent from the initial number of bacteria as well as from zinc ... | 1981 | 6790329 |
| human serum precipitins to human dental plaque and oral bacteria. | | 1981 | 6791620 |
| [studies on the relationship between staphylococcus aureus inhabited in human and staphylococcal food poisoning. 2. a long-term observation of staphylococcus aureus isolated from mouth washings of healthy adults (author's transl)]. | | 1981 | 6799595 |
| [studies on relationship between staphylococcus aureus inhabiting human and staphylococcal food poisoning. 3. the outbreaks of food poisoning due to rice ball contaminated with staphylococci inhabiting human (author's transl)]. | | 1981 | 6801152 |
| isolation and characterization of circulating immune complexes in cystic fibrosis. | a methodology for the isolation and immunologic characterization of igg-containing circulating immune complexes (igg-cic) as detected by the 125iclq-binding assay (clqba) is described. we applied this methodology to sera from patients with cystic fibrosis (cf), both positive and negative for igg-cic. we used latex-fixation-positive rheumatoid arthritis sera and normal human sera as positive and negative controls, respectively. all igg-cic-positive serum samples from cf patients were found to con ... | 1982 | 6804143 |
| antimicrobial activity of topical anaesthetic preparations. | eight commercial topical anaesthetic preparations were tested for their ability to inhibit microbial growth in vitro by incubating serial dilutions with each of 4 micro-organisms. in addition corneas of mice were infected with staphylococcus aureus, and the effect of the anaesthetics on isolation rates of bacteria was investigated. the preparations were shown to have a wide range of antimicrobial activity, correlating both with the active agents and the preservatives. we suggest that some prepar ... | 1982 | 6805500 |
| labeling of rh antibodies on solid-phase protein a. | rh0(d) antibodies which retain immune specificity after radiolabeling were prepared by a procedure which does not require igg isolation from serum, requires 10-fold less isotope than conventional techniques and yields antibody solutions of defined composition. the method involves radioiodination of igg on immobilized protein a, depends on employing human red cells reduced in surface cytophilic igg, and exploits the inability of goat igg to interact with staphylococcus aureus protein a. the techn ... | 1982 | 6806390 |
| polyclonal response of human lymphocytes to bacterial cell walls, peptidoglycans and teichoic acids. | it has been found earlier that many bacteria are polyclonal activators of human lymphocytes. this phenomenon was further analysed by preparing cell walls, peptidoglycans and teichoic acids from staphylococcus aureus wood 46 and bacillus subtilis and studying their capacity to stimulate human adult and newborn lymphocytes to proliferate and to produce leucocyte inhibitory factor (lif). all these bacterial surface components acted as polyclonal activators. in our opinion these findings further str ... | 1982 | 6807836 |
| crosslinkage of antibodies to staphylococcal protein a matrices. | crosslinkage of anti-human albumin (anti-hsa) with varying concentrations of glutaraldehyde to staphylococcus aureus cowan 1 (spa-staph) and to staphylococcal protein a-sepharose (spa-sepharose) was tested. a concentration of 0.0075% glutaraldehyde was found efficient for an almost complete covalent binding of igg to the matrices. the antibody activity of crosslinked anti-hsa spa-staph and anti-hsa spa-sepharose was more than 60 and 90% respectively compared with the corresponding noncrosslinked ... | 1983 | 6827106 |
| selenium and immune functions in humans. | earlier animal experiments have shown that selenium depletion may decrease immune functions. in this human study, 40 volunteers from a population with low serum selenium concentrations were supplemented with selenium or placebo for 11 weeks. blood samples were drawn at intervals for analysis of selenium status and immune function. at the end of the supplementation period, plasma selenium levels were 74 ng/ml in the placebo group and 169 ng/ml in the supplemented group. the improvement in seleniu ... | 1983 | 6862626 |
| a radiolabeled staphylococcal protein a assay for detection of anti-erythrocyte igg in warm agglutinin autoimmune hemolytic anemia of dogs and man. | a cell wall protein from staphylococcus aureus, protein a, (spa) has been shown to have the ability to bind the fc region of most mammalian igg molecules. this study uses this unusual property as the basis for a quantitative assay for erythrocyte (rbc) bound antibodies. test serum is incubated in a suspension of normal rbc's. the cells are then washed and incubated with 125iodine-labeled spa (125i-spa). after incubation cells are pelleted and bound radiolabeled spa counted. this procedure has be ... | 1983 | 6868334 |
| in vitro evaluation of the bactec resin-containing blood culture bottle. | the bactec resin-containing blood culture bottle (16b; johnston laboratories) was designed to bind antibiotics and to support bacterial growth. however, only modest increases in the recovery rates of pathogens have been found in clinical studies. this in vitro study evaluated the ability of the 16b to recover organisms from human serum containing clinically achievable concentrations of antibiotics. escherichia coli, enterobacter cloacae, klebsiella pneumoniae, pseudomonas aeruginosa, haemophilus ... | 1983 | 6874903 |
| modulation of polymorphonuclear leukocyte function by doxorubicin (adriamycin) and alpha tocopherol (vitamin e). | the effect of various concentrations of adriamycin alone and adriamycin plus vitamin e on human polymorphonuclear leukocyte (pmnl) function was determined. aliquots of pmnls were incubated at 37 degrees c with saline or vitamin e and then exposed to adriamycin. a significant decrease in 1-14c glucose utilization and oxygen consumption by zymosan stimulated pmnls occurred when pmnls were incubated with 1, 10, 20, 1000 and 200 micrograms/ml concentrations of adriamycin. preincubation of the pmnls ... | 1983 | 6876145 |
| purification and characterization of the 47,000-dalton protein phosphorylated during degranulation of human platelets. | secretion of platelet granule constituents is closely associated with the phosphorylation of a cytosol polypeptide of mr = 47,000 that we have called p47 (haslam, r. j., lynham, j. a., and fox, j. e. b. (1979) biochem. j. 178, 397-406). this polypeptide is a substrate of ca2+-activated phospholipid-dependent protein kinase (kawahara, y., takai, y., minakuchi, r., sano, k., and nishizuka, y. (1980) biochem. biophys. res. commun. 97, 309-317). two-dimensional gel electrophoresis of protein from hu ... | 1983 | 6885823 |
| humoral reactions in human endocarditis due to streptococcus bovis: evidence for a common s bovis antigen. | humoral reactions to native culture filtrates of streptococcus bovis were studied in patients with endocarditis by means of two-dimensional crossed immunoelectrophoresis. serum from patients with s bovis endocarditis produced eight different precipitin arcs. lex antigen cross-reacted with sera from patients with viridans streptococcal endocarditis, and ta antigen cross-reacted with ribitol teichoic acid-positive sera from patients with staphylococcus aureus bacteremia. one antigen, common (c), w ... | 1983 | 6886490 |
| in vitro synthesis of human brain proteins including tubulin and actin by purified postmortem polysomes. | polysomes were prepared from human brain tissue 2-6 h postmortem; the polysomes were active in a cell-free protein synthesis system containing rabbit reticulocyte factors. protein synthesis was totally dependent upon added mgcl2, atp, the reticulocyte factor fraction, and the human polysome fraction. human brain proteins synthesized in the presence of l-[35s]methionine were analyzed by one- and two-dimensional polyacrylamide gel electrophoresis. over 250 proteins were synthesized and they extend ... | 1981 | 6894159 |
| use of immunoglobulin-loaded protein a-bearing staphylococci as a primary solid phase immunoadsorbent in radioimmunoassay. | protein a-bearing formalinized heat-inactivated staphylococcus aureus bind rabbit 125i-igg with high capacity of igg for antigen. the affinity of immobilized igg for antigen is equal to the affinity of soluble antibody, and the capacity for antigen approximates the capacity of soluble antibody for antigen. 125i-igg bound to high affinity sites on bacteria is not substantially displaced in the presence of human serum after 4 h at 4 degrees c, but rabbit immunoglobulin can displace bound rabbit ig ... | 1980 | 6965378 |
| effect of cyclosporin a on t-dependent and t-independent immunoglobulin synthesis in vitro. | an immunosuppressive cyclic polypeptide, cyclosporin a (cya), isolated originally from two species of fungi, prolongs organ allograft survival in several species. however, virtually nothing is known about the pharmacokinetics of this substance and its mode of action in man. we have quantified the impact of cya on t-dependent and t-independent immunoglobulin (ig) synthesis of human blood leukocytes in vitro, and on the proliferating capacity of the interacting lymphoid cell populations--t cells, ... | 1980 | 6968408 |
| human lymphocyte colony formation: a comparison of stimulation with intact staphylococcus, staph, protein a, pokeweed mitogen and phytohemagglutinin. | the efficacy of various mitogens for growing human lymphocyte colonies in semisolid agar cultures was tested. protein a as well as phytohemagglutinin (pha) exhibited a strong stimulatory capacity in contrast to cowan i strain of staphylococcus aureus (cowan i). pokeweed mitogen (pwm) was effective and generated a sizeable number of colonies. plating efficiency of lymphocytes from different donors and for the same donors cultured on different days were highly variable. regardless of mitogen, colo ... | 1980 | 6969477 |
| low responsiveness of human neonatal lymphocytes to a b-cell mitogen, staphylococcus aureus cowan i (spa coi). | responses of neonatal and adult lymphocytes to various mitogens were studied. lymphocytes from umbilical cord blood (ucb) responded well to both phytohemagglutinin and concanavalin a, and also to pokeweed mitogen and staphylococcus aureus protein a. the responses of ucb lymphocytes to these mitogens were not significantly lower than those of adult peripheral blood lymphocytes (pbl). in contrast, ucb lymphocytes showed only a minimal response to killed staphylococcus aureus cowan i (spa coi), a p ... | 1980 | 6971389 |
| polyclonal activation of human peripheral blood b lymphocytes by formaldehyde-fixed salmonella paratyphi b. i. immunoglobulin production without dna synthesis. | a "new" polyclonal activator of human peripheral blood b cells, formaldehyde-fixed salmonella paratyphi b, is described. this bacterium does not stimulate cell proliferation as measured by incorporation of tritiated thymidine but does stimulate a subpopulation of b cells to secrete large amounts of igm, igg, and iga in 7-day cell cultures. the immunoglobulins (ig) produced by cells responding to s. paratyphi b are not specific antibodies against the bacterial antigens. in comparison with other b ... | 1981 | 6972434 |
| polyclonal activation of human peripheral blood b lymphocytes by formaldehyde-fixed salmonella paratyphi b. ii. heterogeneity of b lymphocytes. | b-cell 'activation' in cultures stimulated with pokeweed mitogen (pwm), staphylococcus aureus strain cowan i, or formaldehyde-fixed salmonella paratyphi b (spb) was evaluated by enumeration of cells secreting immunoglobulin (ig) and by quantitation of ig released into culture supernatants. a dissociation between these two values was found after day 6 in cultures activated with pwm or spb, suggesting that ig-secreting cells (isc) are heterogeneous in terms of ig secretion rate. generation of isc ... | 1982 | 6979775 |
| protein a reactivity of lymphocytes from some patients with chronic lymphocytic leukaemia mediated by an interaction with the f(ab')2 region of surface immunoglobulin. | peripheral blood lymphocytes (pbl) from 15 of 38 patients with chronic lymphocytic leukaemia (cll) were capable of forming rosettes with human erythrocytes coated with staphylococcal protein a (spa-hrbc). pbl from seven patients also showed a marked proliferative response after stimulation with staphylococcus aureus bacteria strain cowan i (cowan staph). the spa-rosetting of cll cells was inhibited by incubation with f(ab')2 fragments of anti-immunoglobulin (ig) antibodies. in addition, incubati ... | 1982 | 6979776 |
| enhancement of human b lymphocyte differentiation in vitro by thyroid hormone. | a study was made of the regulatory influence of a thyroid hormone, triiodothyronine (t3), on human blood t- and b-lymphocytes responses in vitro. blood lymphocytes were stimulated with the t-cell mitogens concanavalin a (con a) and phytohaemagglutinin (pha), with the t-dependent b-cell activator pokeweed mitogen (pwm), and with the t-independent b-cell activator staphylococcus aureus cowan i bacteria in the presence of various hormone concentrations. t3 did not stimulate t-cell proliferation. th ... | 1982 | 6980446 |
| proliferative responses of normal human b lymphocytes. development of an assay system for human b cell growth factor (bcgf). | a simple and reproducible system for inducing and measuring proliferation of normal human peripheral blood b lymphocytes was developed and employed as an assay for bcgf activity contained in supernatants of cultured human mononuclear cells. sac was used to activate human peripheral blood b cells to develop into blasts with low levels of proliferation, and a marked synergistic effect on the proliferation of these activated b cell blasts was demonstrated when various culture-derived supernatants c ... | 1982 | 6980918 |
| specific serum ige antibodies to bacterial antigens in allergic lung disease. | a radio-allergosorbent test (rast) to measure specific ige antibodies in man to whole bacterial cells of streptococcus pneumoniae, staphylococcus aureus and haemophilus influenzae was developed to investigate different well-defined lung diseases (chronic bronchitis, allergic bronchopulmonary aspergillosis (abpa), bronchial asthma allergic rhinitis, cystic fibrosis) and also in urticaria as compared with non-atopic blood donors. in addition, total ige values and skin prick tests were assessed in ... | 1982 | 6982789 |
| peptide mapping by limited proteolysis in sodium dodecyl sulfate of the main intrinsic polypeptides isolated from human and bovine lens plasma membranes. | | 1980 | 6988013 |