| [digestive and cutaneous colonization of staphylococcus aureus, pseudomonas aeruginosa and corynebacterium xerosis in germ-free hairless mice]. | regardless of the method of germ-free hrs mouse contamination, the kinetics of staphylococcus aureus and pseudomonas aeruginosa cutaneous colonization was parallel to the kinetics of intestinal colonization; corynebacterium xerosis, however, was unable to colonize the skin or the gastrointestinal tract. there was little variability in intestinal and cutaneous colonization; this was quite different from observations in man and hairless holoxenic mice. it is possible to use these experimental mode ... | 1985 | 3936400 |
| kinetics of il-2 and interferon-gamma production, expression of il-2 receptors, and cell proliferation in human mononuclear cells exposed to staphylococcal enterotoxin a. | staphylococcal enterotoxin a (sea) at picogram amounts induces high levels of interleukin 2 (il-2) and interferon in human mononuclear cells. sea is a stronger inducer of il-2 than phytohemagglutinin, leukoagglutinin, and concanavalin a. the il-2 induction is very rapid with maximal levels being reached after 18 to 24 hr. the il-2 concentration decreases rapidly and almost no il-2 activity can be detected in supernatants of cells cultured for 3 days or more. maximal dna synthesis is recorded 3 d ... | 1985 | 3939109 |
| an in vitro investigation of the intracellular bioactivity of amoxicillin, clindamycin, and erythromycin for staphylococcus aureus. | the intraphagocytic bioactivities for staphylococcus aureus of amoxicillin, clindamycin, and erythromycin (0.0075-20 micrograms/ml) were measured in human polymorphonuclear leukocytes (pmnls) with the combination of a fluorochrome microassay and a radioassay. pmnls with normal or depleted membrane-associated oxidative metabolism were used to investigate the interactions that may occur between the intrinsic o2-dependent antimicrobial systems of human phagocytes and antimicrobial agents in the eli ... | 1986 | 3950443 |
| role of fibronectin in human monocyte and macrophage bactericidal activity. | fibronectin is a high-molecular-weight glycoprotein found as a soluble dimer in plasma and as an insoluble multimer in tissues. it has been proposed that plasma fibronectin facilitates phagocytic removal of lysed cells and damaged tissues. fibronectin binds avidly to several species of gram-positive bacteria and enhances staphylococcal and streptococcal attachment to cultured cells. determination of whether fibronectin will enhance the bactericidal activity of monocytes and macrophages has not b ... | 1985 | 3972444 |
| cellular and bacterial toxicities of topical antimicrobials. | cellular and bacterial toxicities of four commonly used topical antimicrobials (1% povidone-iodine, 3% hydrogen peroxide, 0.25% acetic acid, and 0.5% sodium hypochlorite) were assayed in vitro using cultures of human fibroblasts and staphylococcus aureus. all agents tested at full strength killed 100 percent of exposed fibroblasts. fibroblast toxicity exceeded bacterial toxicity with serial dilutions of hydrogen peroxide and acetic acid. dilutions of povidone-iodine (1:1000) and sodium hypochlor ... | 1985 | 3975287 |
| adherence of lysostaphin to and penetration into human monocytes. | the effect of lysostaphin on staphylococcus aureus phagocytosed by monocytes was investigated. the results showed that lysostaphin adheres to monocytes by a temperature-independent mechanism, is not adequately removed from monocytes by washing, and penetrates by means of a temperature-dependent mechanism. in in vitro assays of monocyte function, phagocytosed s. aureus can be killed by lysostaphin after penetration of the cells during incubation or by adhering lysostaphin when the monocytes are d ... | 1985 | 3975574 |
| neutralization of erythroid burst-promoting activity in vitro with antimembrane antibodies. | to investigate the relatedness of soluble and pelletable vesicular erythroid burst-promoting activity (bpa) present in lymphocyte-conditioned medium (lcm), we immunized rabbits with partially purified lymphocyte plasma membranes and tested the antisera for biological and immunologic crossreactivity with lcm and its component fractions. when preincubated with igg purified from post-immune but not from preimmune serum, bpa expression by unseparated lcm, lcm-derived pellets, and supernatants was ab ... | 1985 | 3978232 |
| the cross-linking of human met-hemoglobin with [14c]dimethyl adipimidate. | met-hemoglobin, cross-linked with [14c]dimethyladipimidate (cross-linking span 9 a; 1 a = 0.1 nm), yields four distinct molecular weight products (monomer, dimer, trimer, and tetramer) as observed on sodium dodecyl sulfate - polyacrylamide gels. the dimer species was purified to homogeneity by gel filtration. it was proteolytically degraded using a combination of staphylococcus aureus v8 protease, pepsin, trypsin, and chymotrypsin. the resultant peptides were fractionated using gel filtration an ... | 1985 | 3986666 |
| survival of gram-negative and gram-positive bacteria artificially applied on the hands. | we evaluated the survival of pseudomonas aeruginosa, klebsiella pneumoniae, serratia marcescens, escherichia coli, and staphylococcus aureus, derived from either hospitalized patients or culture collections, on the fingertips of human volunteers. over 99% of the bacteria died within 2 min of the application, and about 10(5) cells remained on the fingers for up to 90 min. | 1985 | 3988907 |
| in vitro translation of mrna from toxocara canis larvae. | 300 micrograms of total rna was extracted from 1 ml of packed toxocara canis larvae by centrifugation through a 5.7 m cesium chloride cushion. 60 micrograms of polyadenylated messenger rna was separated from 300 micrograms of total rna in an oligothymidylic acid-cellulose gel column. the in vitro translation of the mrna, isolated from t. canis larvae, was carried out using the rabbit reticulocyte cell-free translation system. incorporation of [35s]methionine into trichloroacetic acid precipitabl ... | 1985 | 3990707 |
| serological characteristics of coagulase-positive staphylococci isolated from man and animals. | a simplified method allowed staphylococcus aureus, staph. intermedius and coagulase-positive staph. hyicus subsp. hyicus isolated from humans, dogs, monkey, sheep, poultry, rabbits, giant rats (cricetomys gambianus) and other animals to be serotyped. the nine coagulase-positive staphylococcal strains of human origin possessed thermolabile and thermostable agglutinogens. two strains of staph. intermedius of human and canine origins examined had agglutinogen k1k2. the three staph. aureus strains i ... | 1985 | 3997688 |
| in vitro ige-secreting cells in man. i. mitogen-independent and -dependent subpopulations. | in vitro ige secretion by atopic and normal peripheral-blood lymphocytes was examined in culture with pokeweed mitogen or staphylococcus aureus strain cowan-i (stacw) or without mitogen. ige secreted in culture supernatants was measured with double antibody radioimmunoassay. enumeration of ige-secreting cells was made by a protein-a plaque assay. ige was detected in increasing quantities in supernatants of cultured lymphocytes without mitogen up to the 12th day. ige-plaque-forming cells were for ... | 1985 | 4038923 |
| bacterial adherence to human endothelial cells in vitro. | differences in the ability of bacteria to adhere to normal valvular endothelium may account for the predominance of particular species as pathogens in acute endocarditis. an in vitro adherence assay was developed to simulate the host surface encountered in acute bacterial endocarditis by using confluent monolayers of human endothelial cells. adherence of 32 gram-positive and -negative blood culture isolates to this surface was compared. all five staphylococcus aureus strains tested were highly a ... | 1985 | 4044035 |
| synergistic hemolysis exhibited by species of staphylococci. | the synergistic hemolysis reactions of 61 reference strains and 189 clinical isolates representing 17 species of staphylococci were examined on plates of trypticase soy blood agar (bbl microbiology systems, cockeysville, md.). some or all of the strains of staphylococcus aureus, s. epidermidis, s. capitis, s. cohnii, s. haemolyticus, s. hyicus, s. simulans, s. warneri, and s. xylosus produced a delta-hemolysin that gave synergistic, complete hemolysis of washed human, sheep, and ox blood cells i ... | 1985 | 4044799 |
| the expression of capsule in serum-soft agar by staphylococcus aureus isolated from human clinical sources. | staphylococcus aureus isolates from human clinical sources were incubated for various times in modified 110 medium and tested for production of capsule by the serum-soft agar technique. ten (5.7%) of 175 isolates were encapsulated after incubation for 24 h. a more detailed examination of 77 isolates showed that incubation period affected the production of capsule. after 2 h, 31% were encapsulated, but after 6 h and 24 h this decreased to 17% and 4% respectively. rapid passage in vitro induced th ... | 1985 | 4045993 |
| recognition and management of acute suppurative parotitis. | acute suppurative parotitis (asp) is a rare infectious process occurring after surgery or in debilitated patients. this case describes a 41-year-old man who was hospitalized initially for acute bacterial endocarditis. late in the hospital course his recovery was complicated by the development of asp. subsequent cultures of blood and parotid-fluid samples grew pseudomonas aeruginosa. despite early empiric antibiotic therapy followed by more specific therapy with an aminoglycoside, the patient con ... | 1985 | 4053524 |
| susceptibility of human and porcine neutrophils to hypothermia in vitro. | hypothermia may contribute to serious life-threatening infections. an experimental model has been established in pigs in order to study the effects of hypothermia on host bacterial defenses. the function of blood neutrophils from pigs and humans was examined in vitro at 37 and 29 degrees c. bacterial killing of staphylococcus aureus 502a by human neutrophils after 90 and 180 min incubation at 29 degrees c was reduced to 76 +/- 6% and 83 +/- 7% of killing at 37 degrees c. porcine neutrophil killi ... | 1985 | 4058977 |
| the multiplicity of human pancreatic secretory trypsin inhibitor. | four forms of pancreatic secretory trypsin inhibitor (psti; a1, a2, b, and c) were purified from human pancreatic juice. according to sequence results, the primary structure of b was different from that reported earlier (greene, l.j., et al. (1976) method enzymol. 45, 813-825) at two positions, i.e. asn21----asp21, asp29----asn29. a1 and a2 were deamidated forms of b judging from peptide mappings with staphylococcus aureus v8 protease. gln45 in b was replaced by glu in a1 and gln51 in b was repl ... | 1985 | 4086468 |
| hormone-dependent covalent modification and processing of human progesterone receptors in the nucleus. | in situ photoaffinity labeling, which minimizes in vitro incubations and proteolytic artifacts, was used to study the structure of progesterone receptors (pr) in intact t47d human breast cancer cells. these cells, rich in pr, were incubated with the photoreactive progestin [3h]r5020 at 0 degrees c for 3 hr to keep pr in their untransformed state, or at 37 degrees c for 5 min to transform pr and convert them to tight chromatin-binding proteins. the cells, still intact, were then irradiated with 3 ... | 1985 | 4092571 |
| isolation by electrofocusing of two lymphocyte mitogens produced by staphylococcus aureus. | staphylococcus aureus strain da352, grown in a diffusate of todd-hewitt broth, produced two extracellular nondialyzable lymphocyte mitogens having isoelectric points of 5.5 to 5.7 and 8.6 to 9.0. the mitogens were separable from one another by isoelectric focusing and could be isolated free of detectable amounts of other staphylococcal products by ethanol precipitation followed by isoelectric focusing. dose-response curves with both mitogens showed a maximum per cent transformation in the range ... | 1972 | 4118043 |
| the use of protein a-containing staphylococcus aureus as a solid phase anti-igg reagent in radioimmunoassays as exemplified in the quantitation of alpha-fetoprotein in normal human adult serum. | | 1974 | 4137309 |
| prevalence of staphylococcus aureus, phage types 71, 80 & 81 among healthy human carriers and their antibiotics resistance. | | 1974 | 4281416 |
| crystal-violet type a -- strains of human origin and their relation to host-adapted variants of staphylococcus aureus. | | 1973 | 4281736 |
| the hygiene and marketing of fresh cream as assessed by the methylene blue test. a report by a working party to the director of the public health laboratory service. | the hygiene and marketing of fresh cream in england and wales was investigated by a working party of the public health laboratory service (phls) between 1 october 1968 and 31 july 1969. thirty-one cream-producing dairies were visited and observations made in the light of the code of practice published by the milk and milk products technical advisory committee of the ministry of agriculture, fisheries and food, and the scottish home and health department. suggestions are made in this report to st ... | 1971 | 4326247 |
| epicillin: in vitro laboratory studies. | a new semisynthetic penicillin, structurally related to ampicillin, has been assigned the generic name epicillin, 6-[d-2-amino-2-(1, 4-cyclohexadienyl) acetamido]-penicillanic acid. the antimicrobial spectrum and level of activity of epicillin in vitro are similar to those of ampicillin. in studies with recent clinical isolates, these two antibiotics, when compared with carbenicillin, showed consistently higher antimicrobial activity against staphylococcus aureus, streptococcus pyogenes, escheri ... | 1971 | 4343402 |
| immune enhancement of pulmonary bactericidal activity in murine virus pneumonia. | bacterial multiplication in the lung associated with murine sendai virus pneumonia is caused by virus-induced defects in pulmonary bactericidal mechanisms. the nature of this effect has been studied in animals immunized against the challenge bacteria. mice were immunized against proteus mirabilis by intraperitoneal inoculation and by aerosol inhalation. after the development of immunity, mice were infected aerogenically with 10(4) tcid(50) of sendai virus. 7 days later, during the height of the ... | 1973 | 4356001 |
| bactericidal activity of aerobic and anaerobic polymorphonuclear neutrophils. | human polymorphonuclear neutrophils (pmn) were made anaerobic by nitrogen washout (oxygen saturation <1%, eh < -42 mv at ph 7.0), and the ability of the cells to kill bacteria was assayed and compared to the bactericidal activity of aerobic pmn. anaerobic pmn were able to kill staphylococcus epidermidis, enterococcus, viridans streptococci, pseudomonas aeruginosa, peptostreptococcus anaerobius, bacteroides fragilis, clostridium perfringens, and peptococcus magnus normally. organisms that were no ... | 1974 | 4361295 |
| in vitro antimicrobial activity and human pharmacology of cephaloglycin. | serum and urine concentrations of cephaloglycin (an orally absorbed derivative of cephalosporin c) were determined in normal volunteers and in patients. the in vitro activity of cephaloglycin was also studied. all strains of group a streptococci (streptococcus pyogenes) and diplococcus pneumoniae were inhibited by 0.4 mug of cephaloglycin per ml. eighty per cent of the staphylococcus aureus strains and about 50% of the escherichia coli and proteus mirabilis strains were inhibited by 1.6 mug of c ... | 1968 | 4385749 |
| anti-gamma globulins and chronic infection: comparative studies of the immune response to various bacteria and gamma globulin preparations. | a study of the relationship of clinical states associated with prolonged infection (bacterial endocarditis and osteomyelitis) and generation of serum anti-gamma globulins was made with particular reference to quantitative amounts of staphylococcal protein a in various infecting strains. no correlation between individual strain amounts of protein a and presence of anti-gamma globulins was detected. thirty-eight rabbits were immunized intravenously with various strains of bacteria (staphylococcus ... | 1972 | 4404684 |
| infection and immunoglobulin concentrations in chediak-higashi mice. | the chediak-higashi syndrome (chs) has been reported in man, cattle, mink, and mice. chs humans and cattle have an increased incidence of pyogenic infections, whereas chs mink are more susceptible to aleutian disease. age- and sex-matched groups of chs mice (mutant strain) and c57 bl/6n (parent strain) were challenged with candida albicans, escherichia coli, klebsiella pneumoniae, and staphylococcus aureus intravenously and streptococcus pneumoniae intraperitoneally. a significant increase (p < ... | 1974 | 4601767 |
| in vitro studies of tobramycin, an aminoglycoside antibiotic. | tobramycin is an aminoglycoside antibiotic which has excellent antibacterial activity against pseudomonas, staphylococcus aureus, and many members of the enterobacteriaceae. most strains of serratia, providence, streptococcus, and diplococcus pneumoniae were resistant to concentrations of tobramycin which could be achieved in man. tobramycin was effective against certain pseudomonas strains resistant to gentamicin. the growth medium used to determine the inhibitory level of tobramycin had a sign ... | 1972 | 4670427 |
| scanning electron microscopy of the attachment of human polymorphonuclear leukocytes to staphylococcus aureus. | | 1973 | 4707311 |
| virulence of two mastitis strains of staphylococcus aureus in bovine skin: enhancement by growth in high carbohydrate-high salt medium or in raw milk. | cows were inoculated intradermally with two strains of staphylococcus aureus, and the severity of the resulting skin lesions was assessed on the basis of size, degree of necrosis, and in some cases by measuring the area of inflammation in histological preparations of excised skin. at least 10(6) colony forming units were required to produce a detectable skin lesion, indicating that the cow has a resistance to staphylococcal skin infection comparable to man and rabbit. the severity of the lesions ... | 1973 | 4716546 |
| modified microbiological assay for rapid estimation of antibiotic concentrations in human sera. | antibiotic concentrations in human sera were estimated in 5 to 6 hr by a modified microbiological assay. by using staphylococcus aureus and streptococcus pyogenes as the assay organisms, the seeded assay plates were preincubated for 2 to 6 hr and then were stored at 4 c until used for assay. paper discs saturated with the specimen were placed on the preincubated assay plates with reference discs saturated with known concentrations of antibiotic. after 5 to 6 hr of incubation, zones of antibacter ... | 1969 | 4896101 |
| heterogenetic antigens of gram-positive bacteria. | chorpenning, frank w. (the ohio state university, columbus), and matthew c. dodd. heterogenetic antigens of gram-positive bacteria. j. bacteriol. 91:1440-1445. 1966.-soluble antigens obtained by various methods from gram-positive bacteria were used to modify erythrocytes whose hemagglutinating reactions with immune rabbit sera and normal human sera were then studied. antigens from all gram-positive organisms studied except corynbacteria altered red cells, causing them to react with specific bact ... | 1966 | 4956340 |
| serological activity of protein a of staphylococcus aureus: the precipitinogen as an antigen for determining antibodies by the passive hemagglutination test. | tanned sheep erythrocytes have been considered incapable of sensitization with the precipitinogen of protein a of staphylococcus aureus, so that the activity of this antigen in serological reactions has so far been studied by means of the agar diffusion test (adt) only. the precipitinogen of the protein a in this study was found to become attached to the tanned erythrocytes and to sensitize them for the passive hemagglutination test (pht). it was determined that, in contrast to nonspecific react ... | 1968 | 4969604 |
| ammonium sulfate coprecipitation antibody determination with purified staphylococcal enterotoxins. | the ammonium sulfate coprecipitation technique of farr was applied in a study of the purified enterotoxins of staphylococcus aureus. ammonium sulfate coprecipitation of iodine-131-labeled enterotoxins a, b, and c, with the use of a 1.6 m concentration of (nh(4))(2)so(4), revealed differences in the antigen-binding capacity of normal and immune rabbit sera for the enterotoxins. the coprecipitation technique provided a quantitative test for detecting antibody to enterotoxin that was more sensitive ... | 1969 | 4979439 |
| polyacrylamide gel identification of bacterial l-forms and mycoplasma species of human origin. | polyacrylamide gel electrophoretic patterns of acidified phenol extracts prepared from whole cells can be used for the identification of bacterial l-forms and mycoplasma species of human origin. ten human mycoplasma serotypes and eight l-forms belonging to five different genera were studied. the gel patterns were sufficiently distinct and reproducible that it was possible not only to identify l-forms at the genus level (group with streptococci) and different mycoplasma serotypes but also to diff ... | 1971 | 4994354 |
| protein a from staphylococcus aureus xi. fixation of human complement and of complement from guinea pig and rabbit. | | 1971 | 4999790 |
| effect of streptococcus pyogenes on tissue cells. | human tissue cell lines from each of the three primary germinal sources, ectoderm (conjunctiva and carcinoma of the buccal mucosa), entoderm (intestine and liver), and mesoderm (heart and monocytes) were inoculated with group a streptococcus pyogenes, staphylococcus aureus, and group d streptococci and were then observed. in addition, the effect of these bacteria on mouse fibroblasts was studied. all of the cell lines appeared to be equally susceptible to damage, but damage to the cells by s. py ... | 1967 | 5337771 |
| flucloxacillin, a new isoxazolyl penicillin, compared with oxacillin, cloxacillin, and dicloxacillin. | flucloxacillin, a new isoxazole penicillin, is active against penicillinase-producing strains of staphylococcus aureus and is well absorbed in man after oral and intramuscular administration. compared with isoxazole penicillins in current clinical use-namely, oxacillin, cloxacillin, and dicloxacillin-flucloxacillin has proved as active against gram-positive cocci, including penicillin-resistant staphylococci. the extent of binding of flucloxacillin to the protein of human serum was similar to th ... | 1970 | 5481218 |
| human plasma and the antibacterial effect of peritoneal dialysis solutions. | the influence of human plasma on the antibacterial effect of solutions for peritoneal dialysis was studied. the solutions contained 43 meq per litre of either acetate or lactate as the source of base. enough pooled human plasma was added to half of each solution to give a total concentration of a gramme of protein per litre. the numbers of viable organisms from 15 clinical isolates each of staphylococcus aureus, escherichia coli, and pseudomonas species were counted before and after incubation i ... | 1971 | 5541232 |
| [on the ecology of staphylococcus aureus on the human skin surface. i. on the phenomenon of the so-called self disinfective power of the skin surface]. | | 1967 | 5599882 |
| [on the ecology of staphylococcus aureus on human skin surface. 3. staphylococcus aureus artificially transferred onto normal skin surface of the forearm and other body regions]. | | 1968 | 5678275 |
| isolation and partial characterization of a staphylococcal leukocyte cytotaxin. | a factor which attracts rabbit polymorphonuclear leukocytes both in vitro and in vivo was isolated from the culture filtrate of a strain of staphylococcus aureus. the activity of the chemotactic factor was independent of fresh serum and it was nondialyzable. incubation of the factor with heat-inactivated human serum markedly inhibited its chemotactic property. the factor was heat-labile (80 c, 10 min) in the crude culture filtrate but was heat-stable when partially purified. | 1969 | 5776515 |
| the use of an antibiotic nasal spray in human nasal carriers of bacteriophage typable, coagulase positive staphylococcus aureus. | | 1965 | 5829271 |
| [results of the determination of resistance to corrosive sublimate in staphylococcus aureus strains of human and animal origin]. | | 1966 | 5979659 |
| cytotoxic potential of stimulated human lymphocytes. | viable and immunologically competent lymphocytes from unsensitized donors damage allogeneic tissue culture cells in the presence of phytohemagglutinin (pha). this cytotoxicity is specific since syngeneic tissue culture cells are not at all or only slightly damaged under similar experimental conditions. in this investigation, the relation between the stimulation of human lymphocytes and their cytotoxicity was studied. chang cells (human liver) served as target cells in all experiments. cell damag ... | 1967 | 6020009 |
| opsonizing factors in human serum. ii. the opsonin and inhibitor of phagocytosis of staphylococcus aureus. | | 1967 | 6057642 |
| murine monoclonal antibodies that identify antigenically distinct subpopulations of human sperm. | murine monoclonal antibodies (moabs) were isolated to characterize antigenically distinct subpopulations of human sperm. spleen cells from balb/c mice immunized with freshly prepared human sperm, were fused with murine p3-ns1-ag4-1 myeloma cells by somatic cell hybridization, and supernatants from the igg-secreting hybridomas were screened by an enzyme immunoassay (eia) for reactivity against fresh human sperm and a panel of human somatic cells. two moabs, sp1d1 and sp7a7, reacted specifically w ... | 1984 | 6084640 |
| epstein-barr virus receptor of human b lymphocytes is the c3d receptor cr2. | identity of the epstein-barr virus (ebv) receptor with the complement receptor type 2 (cr2) was established in three sets of experiments using the monoclonal antibodies, hb-5 and anti-b2, which recognize a mr 145,000 b-lymphocyte membrane protein that is cr2. first, the rank order for binding of fluoresceinated ebv to four lymphoblastoid cell lines (sb, jy, raji, and molt-4) was identical to the rank order for binding of hb-5 and anti-b2 by analytical flow cytometry. second, pretreatment of cell ... | 1984 | 6087328 |
| biosynthesis, transport and processing of myeloperoxidase in the human leukaemic promyelocytic cell line hl-60 and normal marrow cells. | the processing and intracellular transport of myeloperoxidase were studied in the human promyelocytic leukaemia cell line hl-60 and in normal marrow cells labelled with [35s]methionine or [14c]leucine. myeloperoxidase was precipitated with antimyeloperoxidase serum; the immunoprecipitates were subjected to sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and radiolabelled myeloperoxidase visualized by fluorography. during a 1 h pulse, myeloperoxidase was labelled in a chain of apparent ... | 1984 | 6095812 |
| structural and functional comparisons of chicken and human cellular fibronectins. | we have investigated the structural and functional differences between chicken and human cellular fibronectin by comparing the tryptic peptide patterns using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and by analyzing the binding properties of isolated trypsin-resistant polypeptide fragments. although the overall functional organization of chicken and human cellular fibronectins was similar, the tryptic patterns obtained from these two molecules were strikingly different. for exam ... | 1984 | 6146622 |
| use of soluble and staphylococcus aureus-immobilized second antibody compared in a radioimmunoassay for human alpha-fetoprotein. | staphylococcus aureus cells coated with rabbit anti-goat immunoglobulin g were compared with soluble second antibody in a radioimmunoassay for human serum alphafetoprotein. recovery of primary immune complexes with use of the bacterial immunoadsorbant (a) allowed second-antibody incubation time to be shortened from 3 h to 20 min at 37 degrees c, (b) decreased by 80% the amount of second antibody required per assay, thereby significantly reducing assay costs, and (c) provided a convenient and rep ... | 1980 | 6156032 |
| detection of factor ix antibodies by radioimmunoassay: effect of calcium on antibody-factor ix interaction. | a radioimmunoassay for alloantibodies (inhibitors) and heteroantibodies to human factor ix has been developed using radioiodinated human factor ix and formalin-fixed, heat-killed staphylococcus aureus cells (staph a). staph a was used as a solid-phase adsorbent for immune complexes. the assay is specific, shows excellent correlation with factor ix coagulant neutralization assays in detecting alloantibodies (r = 0.98), and is 60 times more sensitive. the staph a method allows rapid separation of ... | 1980 | 6158351 |
| complete amino acid sequence of bovine beta 2-microglobulin. | beta 2-microglobulin has been isolated and crystallized from bovine colostrum and represents the only crystalline form of this protein reported. the complete sequence of bovine beta 2-microglobulin was determined using only one proteolytic enzyme, staphylococcus aureus v8 protease. automated degradation of the intact molecule and two large peptides produced by enzymic digestion provided unequivocal placement of all residues. bovine beta 2-microglobulin, molecular weight 11,630, contains 98 resid ... | 1982 | 6174509 |
| a quantitative enzyme-linked immunosorbent assay for honeybee venom-specific immunoglobulin g. | a quantitative enzyme-linked immunosorbent assay (elisa) for honeybee venom-specific igg is reported. this elisa surmounts the problem of poor reproducibility due to nonparallelism of dilution curves in previously reported elisas. the assay is performed in polyvinyl "u" microtiter plates in which hbv is physically adsorbed to the wells. the antigen is sequentially overlaid with human serum albumin, unknown serum diluted in 10% normal goat serum (ngs), and peroxidase-labeled anti-human igg in ngs ... | 1982 | 6179974 |
| radioactive human lymphoblastoid interferon. one-step purification, regulation of heterogeneous species production and its use for radioimmunoassay. | human lymphoblastoid interferon (alpha type), labeled with [3h]leucine added to virus-induced namalwa cells, was purified quantitatively and in one step from the culture fluid by immune precipitation. the material showed, upon polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, only four radioactive bands with molecular weights ranging from 17000 to 21000, which coincided well with interferon activity. they coincided also with the four interferon protein bands in the el ... | 1982 | 6180896 |
| detection of fungemia obscured by concomitant bacteremia: in vitro and in vivo studies. | our recent clinical experience suggested that bacteremia may interfere with the detection of concomitant fungemia when standard blood culture methods are used. to determine the extent to which bacteria may interfere with fungal isolation from blood cultures, an in vitro model simulating blood cultures taken during concomitant fungemia and bacteremia was created. each of six bacteria (staphylococcus epidermidis, staphylococcus aureus, streptococcus faecalis, escherichia coli, klebsiella pneumonia ... | 1982 | 6186687 |
| immunochemical characterization of a human high molecular weight--melanoma associated antigen identified with monoclonal antibodies. | sodium dodecyl sulfate polyacrylamide gel analysis of a high molecular weight (hmw) human melanoma associated antigen (maa) defined by murine monoclonal antibodies revealed a number of distinct polypeptides ranging from 80,000 up to 280,000 daltons, in addition to an extremely heterogeneous group of components distributed over a wide range in apparent molecular weight (300,000-700,000 daltons). the 280,000 dalton and the larger heterogeneous molecular weight material are glycosylated since they ... | 1983 | 6188530 |
| monoclonal antibody against the n-terminal end of human plasma fibronectin. | purified human plasma fibronectin was digested with cathepsin g and the degradation products were tested for reactivity towards a monoclonal antibody. in an immunoblotting assay, after sodium dodecyl sulphate/polyacrylamide-gel electrophoresis of the digestion products, the 85 000-mr and 72 000-mr gelatin- and heparin-binding fragments as well as the n-terminal 30 000-mr heparin-binding fragment reacted with the antibody, whereas the 64 000-mr gelatin- and heparin-binding fragment did not. in en ... | 1983 | 6194791 |
| human b cell activation and cell cycle progression: stimulation with anti-mu and staphylococcus aureus cowan strain i. | the responses of resting human b lymphocytes to a variety of activation signals were studied. human tonsillar b lymphocytes were separated according to size by countercurrent elutriation. the small b lymphocytes were then stimulated in vitro with various concentrations of anti-mu antibody in the presence or absence of b cell growth factor (bcgf) or with staphylococcus aureus cowan strain i (sac). cellular volume changes and rna synthesis were measured over the first 24 h of stimulation and were ... | 1984 | 6199210 |
| deoxyadenosine modulates human suppressor t cell function and b cell differentiation stimulated by staphylococcus aureus protein a. | adenosine deaminase (ada) deficiency and the resultant accumulation of deoxyadenosine (adr) are associated with profound t cell dysfunction and variable b cell dysfunction in vivo. we examined the effects of adr on the in vitro function of normal human peripheral blood b and t lymphocytes whose ada activity was inhibited by 2'-deoxycoformycin. we found that okt8+ t cell-mediated suppression of spa-induced ig production was markedly reduced by concentrations of adr (3 to 10 microm) that did not a ... | 1984 | 6199411 |
| spontaneous and pokeweed mitogen-induced in vitro igg production specific for s. aureus cell wall determinants in man. | a system has been established to produce in vitro igg specific for cell wall determinants of s. aureus by human peripheral blood mononuclear cells (pbmc). pbmc of healthy individuals, of patients with s. aureus infections and of patients with other bacterial infections were cultured for 12 days. in the culture supernatants (sn) total igg was determined by a competitive ria, and igg to purified cell walls (pcw) of s. aureus strain h by a two step elisa. pbmc of 11 healthy persons produced anti-pc ... | 1984 | 6204800 |
| staphylococcus aureus peptidoglycan induces histamine release from basophil human leukocytes in vitro. | whole killed cells, cell walls, and peptidoglycans of staphylococcus aureus were found to release histamine from human leukocytes and isolated rat mast cells in vitro. the histamine-releasing capability increased in the order of whole bacteria, cell walls, and peptidoglycans. peptidoglycan was found to release histamine by a nonimmunological mechanism, as demonstrated by release in cells deprived of surface immunoglobulins, whereas whole bacteria and cell walls seemed to operate both by immunolo ... | 1984 | 6209223 |
| moxalactam concentration in human aqueous humor after intravenous administration. | two grams of moxalactam disodium was administered intravenously to 20 patients before cataract extraction. mean aqueous humor concentrations of moxalactam were 0.92, 0.87, 1.98, 2.13, and 1.24 micrograms/ml 30 minutes and 1, 2, 4, and 6 hours, respectively, after treatment. adequate levels against staphylococcus aureus and staphylococcus epidermidis were not achieved. with the exception of pseudomonas species, therapeutic levels three to ten times greater than the median minimum inhibitory conce ... | 1982 | 6213219 |
| induction of human immunoglobulin secretion. ii. t lymphocyte dependency and radiosensitivity of t-cell help for induction of b-cell differentiation by staphylococcus aureus strain cowan i. | | 1982 | 6215128 |
| varicella zoster and staphylococcal toxic shock syndrome in a young man. | | 1983 | 6218314 |
| ifn-alpha-induced modulations of the events in human mixed lymphocyte cultures. | autologous mixed lymphocyte cultures (amc) with t-enriched subset of human blood lymphocytes as responders and b-cells or plastic adherent cells as stimulators and allogeneic mixed lymphocyte cultures (mlc) were assayed for blastogenesis and generation of cytotoxic potential. the activated cells lyzed k562 and daudi, autologous and allogeneic pha-blasts. the amc population affected the autologous and allogeneic blasts at a similar strength and there was no indication for selective effects. b-bla ... | 1982 | 6219067 |
| suppression of in vitro lymphocyte dna synthesis by killed pseudomonas aeruginosa. | whole antibiotic-killed classic pseudomonas aeruginosa organisms elicited human lymphocyte [3h]thymidine (tdr) uptake in vitro after 5 days in culture. however, high concentrations of the same preparation did not elicit [3h]tdr incorporation. the investigation of this lymphocyte unresponsiveness revealed that a high dose of p. aeruginosa, when added to lymphocyte cultures together with optimal concentrations of lymphocyte activators (e.g., plant lectins or whole killed staphylococcus aureus cowa ... | 1983 | 6219951 |
| okt4/8 ratio in the blood and in the graft during episodes of human renal allograft rejection. | we have analyzed the frequency of t helper (th) and t suppressor/killer (ts/k) lymphocytes in the blood and in the renal allograft during episodes of rejection and during quiescence. monoclonal okt4 and okt8 antibodies were used to mark the th and ts/k cells, respectively. density centrifugation-separated mononuclear leukocytes and facs iv cell sorter or the staphylococcus aureus rosette assay were used to determine the ratio in the blood, with concordant results. fine needle aspiration biopsy ( ... | 1983 | 6220816 |
| effect of norfloxacin on staphylococcus aureus and pseudomonas aeruginosa in broth, serum and in combination with human polymorphonuclear leukocytes. | | 1983 | 6224689 |
| human helper t cell factor(s). iii. characterization of b cell differentiation factor i (bcdf i). | staphylococcus aureus cowan i (sac) induced proliferation of human b cells but did not lead the cells to differentiate to immunoglobulin (ig)-producing cells. the differentiation of sac-stimulated b cells to igg-producing cells was totally dependent on t cells or t cell factor, designated b cell differentiation factor i (bcdf i). bcdf i exerted its effect on b cells between 48 and 72 hr after the initiation of the culture, at which time the 3h-tdr uptake of sac-stimulated b cells had already rea ... | 1984 | 6228579 |
| association of alpha- and beta-subunits during the biosynthesis of beta-hexosaminidase in cultured human fibroblasts. | subunit association of beta-hexosaminidase was studied in intact fibroblasts using antisera that discriminate between free and associated alpha-chains. these were anti-beta-hexosaminidase a (anti-alpha beta), which precipitated all alpha-chains, free or associated; anti-beta-hexosaminidase b (anti-beta beta), which precipitated those alpha-chains that were associated with beta; and anti-alpha-chains, which recognized only monomeric alpha-chains. after biosynthetic labeling, beta-hexosaminidase o ... | 1984 | 6230359 |
| purification and properties of a receptor for the fc-component of immunoglobulin g from streptococcus dysgalactiae. | a receptor for the fc-component (fcr) of immunoglobulin (ig) g was isolated from the cell-free supernatant of a streptococcus dysgalactiae-culture by affinity chromatography on human igg-sepharose. the single-step procedure yielded purified fcr with a specific activity of 1.1 x 10(6) u/mg protein. it had an estimated molecular weight of 78 000 dalton and an isoelectric point between ph 4.0 and 4.5. it was destroyed by pronase e and was sensitive to trypsin. purified fcr gave strong single precip ... | 1983 | 6232781 |
| bactericidal effects of norfloxacin towards bacteria in urine. | norfloxacin produced a reliably bactericidal effect at concentrations from 3 to 90 mg/l against urine pathogens suspended in human urine. these included enterobacteriaceae, pseudomonas aeruginosa, streptococcus faecalis and staphylococci. resistant mutants of staphylococcus aureus were isolated on two occasions (out of 33 experiments). at high concentrations (c. 90 mg/l) the activity was less but this was probably due to the need for a low ph to dissolve the antibiotic. the ph for optimum activi ... | 1984 | 6234275 |
| characteristics and functional capacities of human cord blood granulocytes and monocytes. | cord blood phagocytic cells were characterized with respect to cytochemical activities, fc gamma and c3b receptors, and capacity to phagocytose and kill various species of bacteria. the percentages of peroxidase-positive granulocytes and monocytes from neonates and adults were comparable; the percentage of esterase-positive cord-blood monocytes was about two-thirds of that of adults' blood monocytes. the numbers of cord blood and adults' monocytes with fc gamma and c3b receptors were similar. ph ... | 1984 | 6240017 |
| a fluorometric determination of sphingomyelinase by use of fluorescent derivatives of sphingomyelin, and its application to diagnosis of niemann-pick disease. | we synthesized fluorescent derivatives of sphingomyelin (n-acyl-sphingosylphosphocholine) and used them as substrates for several sphingomyelinases. the following five fluorescent probes, each attached to the terminal carbon atom of the fatty acyl residue, were introduced into sphingomyelin: dansyl, pyrene, carbazole, 4-chloro-7-nitrobenz-2-oxa-1,3-diazole, and anthroic acid. we compared the rates at which the fluoro- and radiolabeled sphingomyelins were hydrolyzed. they were the same with the f ... | 1980 | 6243257 |
| functions of sendai virus nucleocapsid polypeptides: enzymatic activities in nucleocapsids following cleavage of polypeptide p by staphylococcus aureus protease v8. | | 1981 | 6258313 |
| determination of iga antibodies to human cytomegalovirus by enzyme-linked immunosorbent assay (elisa). | a sensitive enzyme-linked immunosorbent assay (elisa) is described for detection of iga antibodies cytomegalovirus (cmv). the antigen consisted of a sonicated extract of cmv infected human embryo cells. the tested sera were absorbed with staphylococcus aureus (strain cowan 1) before analysis. rabbit antihuman iga peroxidase conjugate was used to detect human iga bound to viral antigen. in parallel, igm and igg antibodies to cmv were studied by elisa and by the immunoperoxidase antibody to membra ... | 1980 | 6262458 |
| [the activity of imidazole derivatives in the presence of human plasma (author's transl)]. | by warburg technique using yeasts and staphylococci, any decrease in antimicrobial activity of the imidazole derivatives could be excluded in the presence of human plasma. econazole nitrate, the classical topical imidazole derivative, and ketoconazole, an oral active antifungal, were tested. the compatibility between imidazole derivatives and human plasma is of practical importance for obtaining optimal therapeutic results in treating human mycoses. | 1981 | 6267837 |
| comparisons of rotavirus polypeptides by limited proteolysis: close similarity of certain polypeptides of different strains. | the polypeptides of sa11 rotavirus produced in virus-infected cells were analyzed by limited proteolysis, using staphylococcus aureus v8 protease. this clearly distinguished between all the known primary gene products of the virus and allowed relationships between other infected-cell proteins, and between infected-cell and virus structural proteins, to be ascertained. a comparison of the proteolysis cleavage patterns between sa11 rotavirus and the human rotavirus wa was also performed which demo ... | 1981 | 6275113 |
| up-regulation of lactogenic receptors -- an immunological artifact? | we examined whether injection of heterologous hormones for more than one week might evoke a humoral immune response which would simulate receptor induction. male rats were injected daily for ten days with human growth hormone (hgh) or ovine prolactin (oprl), and binding of 125i-hgh and 125i-oprl was examined in serum and in membranes from liver and lung. specific binding of 125i-hgh and 125i-oprl increased in the sera of hgh- and oprl-injected animals, respectively. a marked increase in hgh but ... | 1982 | 6284491 |
| peptide mapping of the human transferrin receptor in normal and transformed cells. | human transferrin receptor protein from various human cell lines in tissue culture (phytohemagglutinin-activated lymphocytes, normal embryonic fibroblasts (wi-38), a choriocarcinoma (be wo), an epidermoid carcinoma (kb), and a metastatic breast carcinoma (mcf-7)) was metabolically labeled with [35s]methionine and purified by two methods. the first method involves affinity chromatography of the transferrin receptor with a transferrin-linked sepharose 4b resin. the second method involves direct im ... | 1983 | 6296151 |
| changes in camp metabolism during phagocytosis of s. aureus by human monocytes. | involvement of cyclic adenosine 3',5'-mono-phosphate (camp) in the phagocytosis of staphylococci by human monocytes was demonstrated by assay of adenylate cyclase and camp phosphodiesterase (pde). monocyte adenylate cyclase and pde activities were assayed on cell homogenates prepared from monocytes isolated by plate adherence. phagocytosis of staphylococci was associated with diminished adenylate cyclase activity, which reached a minimum after 10 min of incubation (p less than 0.05), and an incr ... | 1982 | 6296386 |
| rapid detection of herpes simplex virus and varicella-zoster virus in clinical specimens by the use of staphylococcus aureus rich in protein a. | herpes simplex virus (hsv) type 1 and type 2 and varicella-zoster virus (vzv) were detected in cytospin preparations from clinical material by using specific antisera and staphylococcus aureus, cowan 1 strain, rich in protein a (sra). virus was isolated in tissue culture from 22 of the 30 specimens submitted for examination. eighteen isolates showed cytopathic effect characteristic of hsv infection and 4 of vzv. in the cytospin preparations of the same samples hsv was detected in 15, two contain ... | 1983 | 6306990 |
| [relation between cyclic amp and phagocytosis in human monocytes]. | the involvement of cyclic adenosine 3'-5' monophosphate (camp) in the regulation of human monocyte phagocytosis of staphylococcus aureus in vitro was demonstrated by assay of adenylate cyclase (ac) and camp phosphodiesterase (pde). phagocytosis was associated with diminished ac activity (p less than 0,05) and concurrently increased pde activity (p less than 0,005). transmission electron microscopy provided evidence that these changes coincide with peak phagocytic activity occuring 10-20 minutes ... | 1983 | 6309337 |
| [measurement of alpha 2-macroglobulin in human sera by quantitative inhibition of an acid staphylococcal protease (author's transl)]. | concentrations of alpha 2-macroglobulin could be determined in the sera of 215 blood donors and 94 patients with various internal diseases by quantitative inhibition of an acid protease from staphylococcus aureus, m 135 (fig. 1, 2). the determinations agreed closely with those obtained by immunodiffusion (tab. 1, fig. 3). however, the alpha 2-macroglobulin-measurements by the protease method required only 4 h and 40 microliter serum. this procedure would also be suitable for the determination of ... | 1980 | 6158814 |
| immunoprecipitating human antigens associated with vesicular stomatitis virus grown in hela cells. | vesicular stomatitis virus (vsv) preparations made in hela cells, vsv(hela), appeared to contain non-viral structural proteins. this was suggested by neutralization of the virus with homologous and heterologous antisera made against vsv prepared in different cells. antisera against uninfected hela cells failed to neutralize vsv(hela) but did immunoprecipitate the virus in the presence of staphylococcus aureus. these immunoprecipitated vsv(hela) retained their infectivity, despite the presence of ... | 1983 | 6310874 |
| identification and structural characterization of two incompletely processed forms of the fourth component of human complement. | immunoprecipitates of human c4 from edta-plasma were incubated with [14c]methylamine and analyzed by sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis and fluorography. in addition to finding label in the alpha-chains of the secreted (c4s) and predominant plasma (c4p) forms of c4, two additional molecules with apparent molecular weights of approximately 168,000 (p168) and approximately 125,000 (p125) covalently incorporated methylamine, indicating the presence of an internal thioes ... | 1983 | 6313766 |
| human-human b cell hybridomas from in vitro stimulated lymphocytes of patients with common variable immunodeficiency. | human-human b cell hybridomas have been established from the peripheral blood lymphocytes of patients with common variable immunodeficiency (cvi) by fusion with an hgprt-negative b lymphoblastoid cell line. igm-secreting hybridomas were successfully obtained from cvi lymphocytes after stimulation for 5 days in vitro with a combination of pwm and staphylococcus aureus strain cowan i. fusion of peripheral blood lymphocytes that were stimulated for 5 days in vitro with a single mitogen resulted in ... | 1983 | 6313803 |
| hydroxyl radical scavengers produce similar decreases in the chemiluminescence responses and bactericidal activities of neutrophils. | the addition of hydroxyl radical (.oh) scavengers caused similar decreases in the chemiluminescence responses and killing of staphylococcus aureus 502a by human neutrophils in vitro. | 1984 | 6317572 |
| effects of lysosomotropic amines on human polymorphonuclear leucocyte function. | lysosomotropic agents interfere with lysosome function. we studied the effects of the lysosomotropic amines: lidocaine, diphenylamine and dansylcadaverine on several functions of human polymorphonuclear leucocytes (pmn): enzyme release, phagosome-lysosome fusion, superoxide anion generation upon stimulation with opsonized bacteria, and phagocytosis and killing of opsonized staphylococcus aureus. lidocaine depressed all cellular functions tested. diphenylamine reduced enzyme release and phagosome ... | 1984 | 6319273 |
| [effect of the ingestion of wheat bran on the fecal microbial flora of human donors and of recipient gnotoxenic mice, and on the barrier effects exerted by these flora against various potentially pathogenic microorganisms]. | the effect of bran ingestion on the flora of the human digestive tract was studied using two methods: quantitative enumeration of various microbial populations of the faecal flora, and a demonstration of the antagonistic effect exerted by the faecal flora against various potentially pathogenic bacteria of the environment. since this latter study cannot be effected in human subjects, we used a model constituted by axenic mice inoculated with patients' flora. faecal samples from 3 human donors rec ... | 1984 | 6326642 |
| comparison of platelet fibrinogen receptors on intact and proteolytically-treated platelets by use of an anti-glycoprotein iiia monoclonal antibody (ma 123). | a murine monoclonal antibody (ma 123) was selected by screening 153 supernatants of hybridoma cells secreting anti-human platelet antibodies for their ability to inhibit the fibrinogen-induced aggregation of chymotrypsin-treated platelets. ma 123 inhibited the binding of 125i-fibrinogen to adp-stimulated intact human platelets and to platelets treated with chymotrypsin or pronase. moreover, it inhibited the fibrinogen-induced aggregation of these platelet suspensions. the degree of inhibition wa ... | 1984 | 6328693 |
| [human immune interferon: production and action]. | the most marked production of immune interferon by human peripheral blood leukocytes and splenocytes stimulated with phytohemagglutinin (pha) and staphylococcal enterotoxin a (sea) was shown to be achieved when lymphoid cells are propagated under conditions of constant sparing mixing on roller apparatus at a temperature of 37 degrees +/- 0.5 degrees c. the resulting interferon was sensitive to low ph, thermolabile, inactivated by treatment with trypsin, and not neutralised by antisera to human a ... | 1984 | 6328769 |
| localization of viral-envelope-glycoprotein-binding sites in fibronectin. | purified viral-envelope glycoproteins from influenza a virus were found to bind to two fragments of the fibronectin molecule. human plasma fibronectin was digested by leucocyte cathepsin g, and three different fragments, of mr 30000, 40000 and 12000-140000, with specific binding functions were isolated. micelles of radiolabelled influenza a glycoprotein were allowed to bind to these fragments immobilized on polystyrene micro-titre wells. the c-terminal 120000-14000-mr fragments that carry the ce ... | 1984 | 6331388 |
| response of human b cells to staphylococcus aureus cowan i: t-independent proliferation and t-dependent differentiation to immunoglobulin secretion involve subsets separable by rosetting with mouse erythrocytes. | we separated t-depleted mononuclear cells into subsets by rosetting with mouse erythrocytes and studied proliferation and differentiation responses to staphylococcus aureus cowan i (sac), pokeweed mitogen (pwm), and a combination of the two polyclonal activators. all of the t cell-independent proliferation of unfractionated b cells in response to sac was attributable to mouse erythrocyte rosette-forming cells (bmr+). bmr- cells were not stimulated to proliferate by sac in the presence or absence ... | 1984 | 6332138 |
| the wi-l2-729-hf2 human hybridoma system. stable hybrids at high frequency. | a new system for the rapid production at high frequency (greater than 10(-5] of stable human hybridomas is described. this system is based on a high efficiency fusion variant human lymphoblastoid cell line designated wi-l2-729-hf2 and is comparable in many ways to commonly used murine hybridoma systems. wi-l2-729-hf2 cells fuse at high frequency with mitogen-stimulated human b cells resulting in the rapid appearance (within 2 weeks) of stable hybridomas in hypoxanthine/aminopterin/thymidine (hat ... | 1983 | 6334216 |
| species identification and biotyping of staphylococci by the api staph-ident system. | the api staph-ident system, a commercially available biochemical and chromogenic substrate micromethod, was evaluated as a means for identifying the species and determining the biotypes of human strains of staphylococci routinely encountered in a clinical microbiology laboratory. the species identity of 152 of 188 (80.9%) unique clinical isolates of staphylococci was correctly predicted by this method after 5 h of incubation according to the recommendations of the manufacturer. when results were ... | 1983 | 6339546 |