| microbiol growth in lipid emulsions used in parenteral nutrition. | parenteral nutrition via central venous catheterization is associated with serious risks, especially that of sepsis. lipid emulsion (intralipidsweden), which may be administered peripherally, was evaluated for its potential to support microbial growth. washed cultures of staphylococcus aureus, candida albicans, and three species of gram-negative rods were all capable of multiplying in the emulsion at room temperature. variations in inoculum size did not affect the growth rate. studies comparing ... | 1975 | 982 |
| microbicidal mechanisms of human granulocytes: synergistic effects of granulocyte elastase and myeloperoxidase or chymotrypsin-like cationic protein. | the antibacterial activity of a myeloperoxidase (mpo)-glucose oxidase system was found to be greatly increased by granulocyte elastase, present in azurophil granules of human neutrophils. the mpo-h2o3-mediated killing of both escherichia coli and staphylococcus aureus was potentiated by granuocyte elastase at an acid ph, whereas at ph 7.4 only killing of e. coli was potentiated. the potentiating effect of elastase was not dependent on the enzymatic properties of the protein since it was not abol ... | 1976 | 12111 |
| use of clindamycin-2-phosphate in ophthalmology (author's transl). | clindamycin-2-phosphate was studied to evaluate its possible use in ophthalmology. 1. sensitivity of organisms isolated from human clinical materials: of 44 staphylococcal strains in vitro studied 13 were highly sensitive to 0.1 mug/ml and 28 were resistant to 100 mug/ml or more of clindamycin-2-phosphate. 2. in experiments with rabbits, clindamycin-2-phosphate showed superior penetration into the ocular tissues after intravenous injection than after intramuscular injection. 3. clindamycin-2-pho ... | 1977 | 14269 |
| determinants of lung bacterial clearance in mice after acute hypoxia. | net lung bacterial clearance in normal mice is determined by the balance of in vivo bacterial multiplication on the one hand, and the defense mechanisms of mucociliary clearance and phagocytosis and killing by the oxygen-dependent alveolar macrophage on the other. the bactericidal function of the macrophage is the major component of the defense mechanism. the effect of acute hypoxia on the defense mechanism was studied in mice exposed to aerosols of staphylococcus aureus, escherichia coli, klebs ... | 1977 | 21603 |
| effect of leukocyte hydrolases on bacteria. x. the role played by leukocyte factors, cationic polyelectrolytes, and by membrane-damaging agents in the lysis of staphylococcus aureus: relation to chronic inflammatory processes. | a heat-stable factor present in extracts of human blood leukocytes is capable of lysing young staphylococcus aureus at ph 5.0. lysis is characterized by breakdown of cell-wall components as judged by electron microscopic and biochemical analysis. the leukocyte extracts can be replaced by a variety of agents known to injure cell membranes, e.g., leukocyte cationic protein histone, polymyxin b, colimycin, phospholipase a, and lysolecithin. the mechanisms by which all these agents bring about the d ... | 1977 | 33120 |
| factors causing the clumping reaction of streptococcal strains with human plasma. | fresh isolates of 204 strains of streptococcus haemolyticus, 75 strains of viridans group streptococcus, and 45 strains of streptococcus pneumoniae were studied for their clumping reactions with human plasma. the plasma and serum factors that clumped the streptococcal strains were compared with those that clumped a staphylococcus aureus strain. one hundred eleven strains of s. haemolyticus, 10 strains of viridans group streptococcus, and none of the strains of s. pneumoniae tested were clumped b ... | 1979 | 35572 |
| studies on the relationship between the degradative rates of proteins in vivo and their isoelectric points. | acidic proteins tend to be degraded more rapidly than neutral or basic proteins in rat liver, skeletal muscle, kidney and brain and in mouse liver and skeletal muscle. we now report a similar relationship among soluble proteins from rat lung, heart and testes, and from human fibroblasts and mouse-embryo cells grown in culture. these findings indicate that the correlation between protein net charge and degradative rate is a general characteristic of intracellular protein degradation in mammals. t ... | 1979 | 36075 |
| use of staphylococcus aureus for rapid radioimmunoassay of influenza a virus haemagglutinin. | in a rapid method for the radioimmunoassay (ria) of influenza a virus haemagglutinin, staphylococcus aureus (strain cowan i, czechoslovak state collection no mau 55/64) was used for separation of bound and free antigens. with rabbit and human immune sera, the binding of antigen-antibody complexes to heat-killed, formalin-fixed staphylocci was comparable to the double antibody technique. the time required for the completion of binding reaction was about 10 min compared to 18--24 hr required for d ... | 1979 | 39433 |
| opsonic activity of normal human cerebrospinal fluid for selected bacterial species. | the opsonic activity of normal human cerebrospinal fluid (csf) has not been well defined. in this study, the opsonic activity of normal csf for laboratory and blood culture isolates of staphylococcus aureus, streptococcus pneumoniae, escherichia coli, hemophilus influenzae type b, and neisseria meningitidis was measured by a quantitative assay employing radiolabeled bacteria and polymorphonuclear leukocytes. all isolates of s. aureus, except the wood 46 strain, were opsonized in undiluted csf (> ... | 1979 | 43289 |
| comparative study of erythromycin, amoxicillin and ampicillin antimicrobial activity against human respiratory tract pathogens. | an in vitro test system was used to compare the antimicrobial activity of erythromycin, amoxicillin and ampicillin against respiratory tract pathogens isolated from man. the minimum inhibitory concentrations (mics) of fresh clinical isolates of streptoccus pyogenes, streptocuccus pneumoniae, staphylococcus aureus and haemophilus influenzae to the macrolide and penicillins ranged between 0.01 and 0.9 microgram/ml. the microbes were exposed to each antibiotic for approximately 3 h at 1x,2x and 5x ... | 1979 | 44765 |
| [serological studies on staph. aureus strains from various processes of a disease of human origin (author's transl)]. | this paper deals with serological studies on 332 staph. aureus strains of human origin having been isolated from various sources. on the basis of typing with 17 factor sera, a great number of groups and types was obtained occurring among the strains of several processes of a disease without showing special serological structural formulae which would have been typical for pathological changes of certain localisation. but still deviations have been observed between the strains of most of the serie ... | 1975 | 52246 |
| cytomegalovirus specific igm and igg response in humans studied by radioimmunoassay. | an indirect solid phase micro-radioimmunoassay (ria) was adapted for the measurement of anti-cytomegalovirus class-specific immunoglobulin m (igm) and immunoglobulin g (igg). cytomegalovirus (cmv) antigen (ag) was added to the wells of microtiter plates and desiccated onto the bottom surface of the wells. serial dilution of human cmv antisera were added and allowed to react with the ag. the amount of viral antibody (ab) was determined by measuring the specific binding of 125i-labeled goat anti-h ... | 1976 | 62802 |
| differential staining of bacteria in clinical specimens using acridine orange buffered at low ph. | optimal conditions for acridine orange staining of air dried and methanol fixed bacteria on glass slides were studied. the ph of the staining buffer did not influence the fluorescence of an s. aureus and an e. coli strain at dye concentrations of 25-50 mg per litre. 81 bacterial strains representing 15 different species were stained with acridine orange under standard conditions, all strains showing orange fluorescence. the ph of the buffer influenced markedly the staining patterns of human cell ... | 1977 | 70957 |
| [experience in studying protein a in staphylococcal cultures]. | methods of quantitative and qualitative determination of protein a in staphylococcus cultures were studied comparatively. the maximal number of strains positive by protein a were revealed by means of indirect hemagglutination test with cell extracts. quantitative and qualitative characteristics by protein a can be used in studying the problems of strain and clone heterogeneity of s. aureus. a common antigen was revealed in the reactions with human gamma-globulin in 21 of 38 s. aureus strains, an ... | 1978 | 79281 |
| effects of human alpha-foetoprotein on human b and t lymphocyte proliferation in vitro. | | 1978 | 82499 |
| thermonuclease seroinhibition test for distinguishing staphylococcus aureus from other coagulase-positive staphylococci. | since coagulase-positive staphylococci from animals are heterogeneous, another test is necessary to distinguish staphylococcus aureus from them. staphylococcal thermonucleases appear to be heterogeneous; antisera raised against s. aureus isolated from humans inhibit thermonuclease activity as demonstrated by the metachromatic well-agar diffusion method. the serological specificity of the thermonuclease elaborated by s. aureus of human origin was demonstrated using three antisera and 407 strains ... | 1979 | 85633 |
| [therapy and prophylaxis of experimental staphylococcal nephritis of the rabbit with gamma-globulin and f(ab')2-fragments (author's transl)]. | prophylactic and therapeutic effects of immunoglobulins and their combination with ampicillin were studied in an experimental bacterial nephritis model after i.v. injection of staphylococcus aureus into rabbits. untreated human igg and a preparation containing f(ab')2-pieces were investigated on therapeutic effects. both antibody preparations prevented the lethal purulent inflammation of kidneys when injected immediately before the infection. the course of the pathological process after infectio ... | 1979 | 87206 |
| solid phase radioimmunoassay for quantitation of antigen-specific igg in human sera with 125i-protein a from staphylococcus aureus. | radiolabeled protein a from staphylococcus aureus (staph a) has been used to develop a solid phase, noncompetitive radioimmunoassay for quantitation of specific igg antibody. the assay involves two incubations: first, agarose-insolubilized antigen is mixed with serum samples for 1 to 4 hr during which specific antibody is bound; second, after a washing procedure, the solid phase immune complexes are incubated for 4 to 18 hr with 125i-staph a, during which the radiolabeled detection protein binds ... | 1979 | 87416 |
| bacteriological criteria for feeding raw breast-milk to babies on neonatal units. | aerobic cultures of 207 samples of drip breast-milk from seventy mothers in hospital showed that 6 (3%) were sterile and 170 (82%) contained only the commensal organisms coagulase-negative staphylococci and streptococcus viridans. 25 (15%) grew potential pathogens--staphylococcus aureus in 13 (6%); enterobacteria in 15 (7%); and group-b streptococci in 3 (2%). it is proposed that milk with commensals in any numbers may be considered for use unheated but not milk containing any potential pathogen ... | 1979 | 90814 |
| effect of leukocyte hydrolases on bacteria. xiv. bacteriolytic effects of human sera, synovial fluids, and purulent exudates on staphylococcus aureus and streptococcus faecalis: modulation by cohn's fraction ii and by polyelectrolytes. | normal sera and plasma, derived from humans, calves, rats, rabbits, horses, human synovial fluids, inflammatory exudates, and leukocyte extracts, when sufficiently diluted are highly bacteriolytic for staph, aureus, strep. faecalis, b. sutilis and to a variety of gram-negative rods. on the other hand, concentrated serum or the other body fluids are usually not bacteriolytic for these bacterial species. while the lysis of staph, aureus and b. subtilis by diluted serum is not lysozyme dependent, l ... | 1979 | 92458 |
| bacteria induce lymphokine synthesis polyclonally in human b lymphocytes. | we have studied the ability of various bacteria to stimulate human lymphocytes to produce leukocyte migration inhibitory factor (lif). mononuclear cells from adult and cord blood as well as purified t and b lymphocytes were stimulated with killed bacteria. the culture supernatants were tested for the presence of lif by the agarose migration method. all nine bacterial strains tested activated unseparated mononuclear cells and b lymphocytes but not t cells to produce lif. lif was also present in c ... | 1978 | 98583 |
| immunoglobulin m synthesized by human lymphoblastoid cells: interaction with staphylococcus aureus and protein a. | immunoglobulin m synthesized by a human lymphoblastoid cell line, la173, was found to bind specifically to the protein a-bearing cowan i strain of staphylococcus aureus. the (3h)-leucine-labeled, secreted igm from these la173 cells also formed precipitin complexes with purified protein a. soluble complexes formed at high protein a/igm ratios retained the ability to bind to the bacterial surface. precipitin complexes also were observed in double diffusion ouchterlony gels with a line of identity ... | 1978 | 100556 |
| effect of serum on gram-positive cocci grown in the presence of penicillin. | strains of staphylococcus aureus, streptococcus faecalis, streptococcus bovis, and a strain of streptococcus that produces filaments were grown on agar containing penicillin at concentrations of one-half to 1/10th the minimal inhibitory concentration. these penicillin-exposed organisms as well as untreated control organisms were incubated with human serum of plasma. both serum and plasma produced a remarkable bactericidal effect on the filament-forming streptococcus grown in the presence of peni ... | 1978 | 105057 |
| frequency and fate of human renal allografts contaminated prior to transplantation. | bacterial cultures were done from donor ureter and/or graft perfusion fluid on 92 of 112 patients who had renal transplantations during 1977. three samples of perfusion fluid and 14 samples of donor ureter contained bacteria. in nine cases there was a gram-positive organism, and in 10 cases a gram-negative rod was (among) the contaminant(s). there was no difference in the rate of graft survival, patient survival, or in the frequency of major postoperative complications between the two groups. on ... | 1979 | 107605 |
| staphylococcus aureus opsonization mediated via the classical and alternative complement pathways. a kinetic study using mgegta chelated serum and human sera deficient in igg and complement factors c1s and c2. | staphylococcus aureus opsonization was studied kinetically by: (1) determination of the uptake of [3h]-thymidine labelled bacteria by human pmn's; (2) fluorescent anti-c3 and anti-igg staining of opsonized bacteria; and (3) measuring bacterial complement consumption. maximum opsonization in normal serum occurred within 5 min of incubation. about 80% of staphylococci were then taken up by pmn's, and igg and c3b could be detected on the bacterial surface. in the absence of a functional classical c ... | 1979 | 108204 |
| effects of hyperbaric oxygen upon s. aureus, ps. aeruginosa and c. albicans. | numerous in vitro investigations have reported that prolonged, continuous hyperbaric oxygen (ohp) exposure to certain bacteria and yeast is bacteriostatic. while it is tempting to attribute the lower infection rates reported in ohp-treated patients to this bacteriostatic effect, the duration and intensity of ohp exposure in these experimental studies exceeds that of normal therapeutic use. this study was designed to investigate the effects of human ohp treatment protocols upon the in vitro growt ... | 1979 | 114160 |
| comparison of radioimmunoprecipitation assays for the detection of human anti-tumor virus antibodies. | the demonstration of human antibodies reactive in radioimmunoprecipitation assays (rias) with primate tumor virus (oncornavirus) antigen has implications for a possible previously published negative findings and led to considerable scientific controversy. we feel much of the discrepancy may be of methodological origin. an attempt is therefore made in this communication to resolve these apparent discrepancies by comparing various published parameters of the rias used in the search for human antib ... | 1979 | 117054 |
| characteristics of staphylococcus aureus associated with lysogenic conversion to loss of beta-hemolysin production. | staphylococcus aureus strains 7-8 and 57 that produce beta-hemolysin but not staphylokinase (beta + k-) were lysogenically converted by certain serological group f bacteriophages to the loss of beta-hemolysin production and the gain in staphylokinase production (beta-k+). serological group a phage 42e was found to convert s. aureus strains 7-8(beta-k-) and 57 (beta + k-) to beta - k-. conversion of beta-hemolysin by lysogenization of a serological group a phage has not previously been reported. ... | 1975 | 125146 |
| epidemiology of methicillin-resistant staphylococcus aureus in dairy herds. | sixty-eight methicillin-resistant staphylococcus aureus strains were isolated from mastitis milk samples originating from 20 belgian dairyherds. all these strains appeared to be representatives of one single strain which was probably of human origin. evidence is presented indicating a rapid in vivo evolutionary change in this strain. the following characteristics were found to be variable: the production of beta haemolysin inversely connected with fibrinolysin (staphylokinase) activity; the prod ... | 1975 | 125447 |
| [capsule formation in staphylococcus aureus as a reason for nontypability by phages (author's transl)]. | in staph. aureus strains isolated from human pathological material the frequency of strains which did not react with typing phages (nt-strains) was found to be about 30%. in one half of the nt strains the reaction with typing phages is prevented by a capsule. the capacity for capsule-formation is lost after propagation of the cells in a liquid glycerol-minimal-medium; thus the cells become typable by phages. the capacity for capsule-formation can be restored after intraperitoneal injection into ... | 1975 | 130760 |
| staphylococcal scalded-skin syndrome: development of a primary binding assay for human antibody to the exfoliative toxin. | exfoliative toxin (et) from a phage group ii staphylococcus aureus strain causing staphylococcal scalded-skin syndrome was purified by electrofocusing. ampholytes and salts were removed from the final product by column chromatography on g-50 sephadex. sodium dodecyl sulfate-polyacrylamide gels of the final product yielded a single band upon gel electrophoresis, even when 60 mug of protein was placed in the gels. radiolabeling of the purified toxin with 125i yielded a product that still caused ex ... | 1976 | 131109 |
| trends in the distribution of staphylococcus aureus phage patterns in new york state during 1966-1975. | the phage patterns of 15 790 s. aureus isolates collected from human sources in new york state (exclusive of new york city) during the 10-year period 1966-1975 were analysed. the results showed changes in the distribution of phage groups and a steadily increasing incidence of nontypable s. aureus. | 1976 | 142587 |
| [the mutual transmission of staphylococcus aureus between humans and cattle and the environmental adaptation of hemolysin and fibrinolysin formation]. | in an ecological investigation in 20 dairy herds of cattle we compared the characteristics of strains isolated from nasal swabs of milkers, from the udder of cows and from cases of mastitis in cows. in 7 herds we found definite strains in the nasal flora of the milkers and in the udder-flora of the cows which only differ in the formation of hemolysin and of fibrinolysin. in the other checked characteristics strains from man and strains from cattle show identical patterns. in 5 herds these strain ... | 1978 | 149523 |
| [protein a research in 1200 strains of staphylococcus (using a rapid detection technic by passive hemagglutination with glutaraldehyde treated erythrocytes]. | the technique used was that of passive hemaglutination of red cells of sheep prepared with glutaraldehyde and sensitized by anti-erythrocyte antibodies. a protein-a was not found in s. epidermidis strains, but was present in 96.3 per cent of 689 strains of s. aureus from human, animal and food origins. | 1978 | 150897 |
| phage typing of coagulase-negative staphylococci. | 218 bovine and 116 human strains of coagulase-negative staphylococci and 46 bovine staphylococcus aureus strains were typed with the verhoef-phage set for human staphylococci and the holmberg-set for bovine staphylococci. 22.5% of the bovine strains were lysed by the bovine phages and only 3.2% by the human phages. none of the bovine staph. aureus strains could be typed. 21% of the human strains tested were lysed by the human phages and only 5.2% by the bovine phages. these results clearly demon ... | 1978 | 151457 |
| phage typing of nontypable isolates of staphylococcus aureus using the new phages 94, 95, and 96 and the heat-shock treatment. | of 2,144 isolates of staphylococcus aureus (from human beings in new york state) received in the authors' laboratory during 1975, 992 (46.,%) were nontypable with the use of the international basic set of bacteriophages. these 2,144 isolates were retested with the use of the recently added phages 94, 95, and 96. nearly 60% of the isolates were lysed with one or more of these three phages, including 64% of those previously nontypable, thus reducing the percentage of nontypables to 16.5%. one hund ... | 1978 | 152059 |
| [staphylococcus aureus in poultry--biochemical characteristics, antibiotic resistance and phage pattern (author's transl)]. | in a poultry processing plant in northern germany 1412 swabs were taken from poultry carcasses together with 608 swabs from the personnel. the broilers came from 22 different chicken farms. the swabs taken from the poultry and those taken from the personnel proved to be 35% and 48% staph. aureus positive respectively. the swabs taken from the feathers and from the skin were staphylococcal positive at a higher level (47%) than the swabs taken from the cloaca (19%) and the throat (23%). between 8% ... | 1979 | 159590 |
| determination of toxin-induced leakage of different-size nucleotides through the plasma membrane of human diploid fibroblasts. | human diploid lung fibroblasts were treated with cytolytic bacterial toxins and the nature of the membrane damage was investigated. [3h] uridine was used for differential labeling of cytoplasmic components of small or large molecular size. two principal size categories were achieved by labeling the fibroblasts in either early growth phase or stationary phase, a high-molecular weight ribonucleic acid label and a low-molecular-weight nucleotide label. the size of the labeled molecules was determin ... | 1975 | 164404 |
| sensitive assay for detection of toxin-induced damage to the cytoplasmic membrane of human diploid fibroblasts. | a sensitive assay was developed for detection and quantitation of subtle permeability changes in the cytoplasmic membrane of human diploid fibroblasts. release of the non-metabolizable amino acid [1-14c]alpha-aminoisobutyric acid (aib; molecular weight (103) from the cytoplasm of prelabeled cells was used as an indicator of toxin-induced membrane damage. an optimal procedure for labeling these cells was designed after varying the conditions with regard to ph, temperature, concentration of aib, c ... | 1975 | 169201 |
| organization of phospholipids in human red cell membranes as detected by the action of various purified phospholipases. | 1. the action of eight purified phospholipases on intact human erythrocytes has been investigated. four enzymes, e.g. phospholipases a2 from pancreas and crotalus adamanteus, phospholipase c from bacillus cereus, and phospholipase d from cabbage produce neither haemolysis nor hydrolysis of phospholipids in intact cells. on the other hand, both phospholipases a2 from bee venom and naja naja cause a non-haemolytic breakdown of more than 50% of the lecithin, while sphingomyelinase c from staphyloco ... | 1975 | 169915 |
| synthesis of 5-amino-1-(5-deoxy-beta-d-ribofuranosyl)imidazole-4-carboxamide and related 5'-deoxyimidazole ribonucleosides. | 5-amino-1-(beta-d-ribofuranosyl)imidazole-4-carboxamide (1, aica ribonucleoside) was converted in two steps to 5-amino-1-(5-deoxy-5-iodo-2,3-o-isopropylidene-beta-d-ribofuranosyl)imidazole-4-carboxamide (3) which was hydrogenated in the presence of pd/c to yield 5-amino-1-(5-deoxy-2,3-o-isopropylidene-beta-d-ribofuranosyl)imidazole-4-carboxamide (4). the dehydration of 4 yielded 5-amino-1-(5-deoxy-2,3-o-isopropylidene-beta-d-ribofuranosyl)imidazole-4-carbonitrile (7). the compounds 3, 4, and 7 w ... | 1975 | 172632 |
| suppression of pulmonary antibacterial activity following sendai virus infection in mice: dependence on virus dose. | | 1975 | 173264 |
| hydrogen peroxide production and killing of staphylococcus aureus by human polymorphonuclear leukocytes. | the effects of bacterial neuraminidase on production of hydrogen peroxide (h2o2) and killing of staphylococcus aureus by human polymorphonuclear leukocytes (pmn) were studied. the concentration of h2o2 was measured by the disappearance of scopoletin fluorescence in the presence of horseradish peroxidase. the results indicated that desialylation of human pmn inhibited the stimulation of h2o2 production during phagocytosis. it also markedly impaired the killing of s. aureus. impaired killing of s. ... | 1977 | 189859 |
| the effect of phorbol myristate acetate on the metabolism and ultrastructure of human alveolar macrophages. | in the present investigation we examined the influence of the surface-active agent phorbol myristate acetate (pma) and opsonized heat-killed bacteria (hkb) on oxygen consumption, superoxide release, and glucose oxidation of human alveolar macrophages (am). both pma and hkb produced a surge in oxygen consumption, superoxide release, and oxidation of 1-14c-glucose and 6-14c-glucose by human am. examination of am by electron microscopy following stimulation by these two agents demonstrated membrane ... | 1978 | 207188 |
| interaction of the b95-8 and p3hr-1 substrains of epstein-barr virus (ebv) with peripheral human lymphocytes. | two substrains of the epstein-barr virus derived from the b95-8 and p3hr-1 cell lines were studied for their interaction with human peripheral lymphocytes. it has been previously shown that b95-8 virus has and p3hr-1 virus lacks lymphocyte-transforming ("immortalizing") properties. dna stimulation induced by b95-8 virus showed a good correlation with the number of surface ig-positive cells. p3hr-1 virus added before b95-8 virus completely abolished the stimulation of dna synthesis. it also preve ... | 1978 | 212371 |
| the effect of pneumonia induced in mice with mycoplasma pulmonis on resistance to subsequent bacterial infection and the effect of a respiratory infection with sendai virus on the resistance of mice to mycoplasma pulmonis. | the effect of pneumonia induced by mycoplasma pulmonis in mice on the resistance of the lung to additional bacterial infection was examined. the effect of pneumonia induced by sendai virus on the resistance of mice to m. pulmonis was also investigated and compared with the effect of sendai virus on resistance to staphylococcus aureus. sendai virus infection decreased subsequent resistance to m. pulmonis in proportion to the virus dose. decreased resistance to subsequent s. aureus and m. pulmonis ... | 1978 | 215702 |
| detection of virus and cellular-determined antigens in situ using [125i]protein a and autoradiography. | the present paper describes the use of [125i]protein a, isolated from staphylococcus aureus, in detecting antigen-antibody complexes by autoradiography on single cells. the method is relatively quick, reproducible, potentially more sensitive than immunofluorescence, and should be useful in combination with conventional radioimmuno-assays. we have used it to detect the cellular expression of igm, kappa, lambda, and beta 2-microglobulin, as well as the expression of epstein-barr virus (ebv)-associ ... | 1979 | 227963 |
| effects of sendai virus infection on function of cultured mouse alveolar macrophages. | cultured mouse alveolar macrophages supported the growth of sendai virus (murine parainfluenza i virus), as measured by both a 10-fold increase in extracellular virus titers and development of viral antigens on most of the cells. synthesis of virus continued for at least 1 month without cytopathic effects. macrophage phagocytic activity for candida, staphylococcus epidermidis, and opsonized erythrocytes remained unaffected by the infection, and the ability of the cells to kill s. epidermidis and ... | 1979 | 229749 |
| the bacteriolytic effect of human dentoalveolar purulent exudates and leukocyte extracts. | | 1977 | 273590 |
| neuraminidase alteration of human lymphocyte reactivity to mitogens, antigens and allogenic lymphocytes. | lymphocyte reactivity to phytohemagglutinin (pha) was reduced significantly in whole-blood cultures initiated with nontoxic concentrations of vibrio cholerae neuraminidase (vcn). reduced responsiveness was not due to degradation or inactivation of pha by this enzyme, and the reaction was dependent upon the dose and time at which this mitogen and enzyme were added to the cultures. in cultures from some subjects, vcn also inhibited reactivity to concanavalin a. in contrast, lymphocyte reactivity t ... | 1978 | 283169 |
| protein a from staphylococcus aureus-a mitogen for human t lymphocytes and b lymphocytes but not l lymphocytes. | | 1978 | 304871 |
| activation of human b and t lymphocytes by protein a of staphylococcus aureus. | protein a from staphylococcus aureus, in soluble form or coupled to sepharose beads, acts as a polyclonal b cell activator (pba) for human lymphocytes in blood and spleen. pba activity was demonstrated in spleen cells by the ability of protein a to induce the formation of intracellular immunoglobulin synthesis and to activate polyclonal antibody secretion demonstrated against fluorescein isothiocyanate-coupled sheep erythrocytes in a modified hemolysis in gel assay. more plaqueforming cells (pfc ... | 1978 | 305849 |
| cell mediated immune responses to bacterial antigens on human mucosal surfaces. | | 1978 | 311141 |
| [characteristics of the mitogenic activity of different staphylococcal strains]. | the mitogens of human peripheral lymphocytes were detected in the metabolic products of 59 out of 71 s. aureus strains. the preparations of s. epidermidis were inactive. when stimulated with filtrates of various s. aureus strains, 72-hour lymphocyte cultures were found to have 0--46% of blasts. two-year observation of a strain showed its stable mitogenic characteristics. the mitogenic properties of the preparations did not correlate with their coagulase, alpha-toxic, dermonecrotoxic, cytotoxic, ... | 1979 | 316242 |
| monocyte function in man. | the monocyte-macrophage cell line is an important member of the host defense system. this report describes a series of assays that can be applied routinely in the evaluation of human monocyte function and gives information as to the activity of normal monocytes in these systems. tests were chosen to assess various aspects of monocyte function that give some insight into the host defense status and the degree of "activation" of the monocyte. the assays outlined in this report are relatively simpl ... | 1977 | 318668 |
| [cytolytic activity of human tonsillar lymphocytes]. | the radioisotopic method was applied to the study of cytolytic activity of human tonsil lymphocytes against chick erythrocytes, intact and loaded with streptococcus and staphylococcus antigens. lymphocytes proved to be much more active in the lysis of target cells treated with microbial antigens than of the intact ones. the degree of erythrocytolysis dispension of the tonsillar cells and their treatment with antiglobulin serum. | 1977 | 331794 |
| expression of two differentiation antigens on normal and cultured human t cells. | an antiserum specific for human t lymphocytes (amt) was used to examine patterns of t cell surface antigen expression and to isolate their reactive membrane antigens. by a quantitative adsorption assay, different plateaus of amt reactivity with blood t cells were observed after serial adsorptions with individual t cell lines. molt-3 cells removed 95% of amt activity to blood t cells whereas molt-4 removed 70% and hsb-2 removed only 30%. a cross-adsorption analysis demonstrated that each of the t ... | 1978 | 355545 |
| the effect of methylmethacrylate on bacterial phagocytosis and killing by human polymorphonuclear leukocytes. | the phagocytic and bactericidal activities of human polymorphonuclear leukocytes in the presence of methylmethacrylate were determined by in vitro techniques. it was found that methylmethacrylate in concentrations as low as 0.156 per cent significantly decreased the ability of leukocytes to phagocytose and kill strains of staphylococcus epidermidis, staphylococcus aureus, and escherichia coli. the evidence indicates that methylmethacrylate impairs the killing more than the phagocytosis by the le ... | 1978 | 359561 |
| protection of phagocytosed bacteria against antimicrobial agents. | antibiotic sensitive staphylococcus aureus phagocytosed by human neutrophil granulocytes were exposed to penicillin g (benzyl penicillin), gentamicin or rifampicin in concentrations from 2.5 u/ml, 2.5 microgram/ml and 0.2 microgram/ml to 250 u/ml, 250 microgram/ml and 20 microgram/ml, respectively. the bacteria were protected from the antibacterial effects of penicillin g and gentamicin. considerable numbers of phagocytosed bacteria remained viable after 24 hours exposure to antibiotic concentra ... | 1978 | 360374 |
| immunofluorescent localization of staphylococcos aureus antigen in acute bacterial endocarditis nephritis. | a 75-year-old man with staphylococcus aureus endocarditis in whom acute diffuse proliferative glomerulonephritis developed is described. the light- and electron-microscopic changes of the glomeruli in this case were identical to those of acute poststreptococcal glomerulonephritis. immunofluorescence revealed deposition of immunoglobulins and complement in the glomeruli. in addition, bacterial antigenic material was demonstrated in the glomeruli by indirect immunofluorescence. these observations ... | 1978 | 362890 |
| antibody-coated protein a-bearing staphylococcus aureus: a versatile and stable immune reagent. | the human beta2-microglobulin antigen-antibody system was used as a model to illustrate the versatility of a microradioimmunoassay technique using protein a-bearing staphylococcus aureus cowan i strain (saci) bacteria as a non-specific immunoadsorbent in place of a second antibody. experimental conditions are described for a sensitive microassay which makes it possible to process large numbers of samples more rapidly and with minimum handling. furthermore, saci coated with specific antibodies by ... | 1979 | 368250 |
| antibody production and dna synthesis of human lymphocyte subpopulations induced by ppd tuberculin. | purified protein derivative (ppd) of tuberculin was found to induce antibody secretion and dna synthesis in human lymphocytes from blood, spleen, tonsil and lymph node. antibody secretion was measured as plaque-forming cells (pfc) against fluorescein isothiocyanate (fitc) coupled sheep red blood cells (srbc) in a haemolysis-in-gel assay. peak antibody secretion by 100 micrograms/ml of ppd was usually seen on day 6 for blood lymphocytes, and varied from day 3 to day 6 for spleen cells. peak dna s ... | 1979 | 385183 |
| lipopolysaccharide and lipid a-induced human blood lymphocyte activation as detected by a protein a plaque assay. | various purified cell wall lipopolysaccharides (lps) from gram-negative bacteria and derivatives of these lps were tested for their stimulatory capacity for human peripheral blood cells. immunoglobulin (ig) production was tested by an indirect plaque-forming cell assay using staphylococcus aureus protein a-coupled erythrocytes and specific anti-ig as developing serum. this method allows the detection of the majority of cells secreting ig of a single class, and the number of plaque-forming cells ... | 1979 | 387423 |
| interactions between human eosinophils and schistosomula of schistosoma mansoni. ii. the mechanism of irreversible eosinophil adherence. | previous work (1)(1) has shown that normal human eosinophils show a preferential capacity, in comparison with neutrophils, to bind to antibody- coated schistosomula of schistosoma mansoni. this effect is attributable to a temperature-dependent function of the eosinophil which renders its binding stable and irreversible by aggregated gamma globulin or staphylococcus aureus protein a. in contrast, the binding of neutrophils is readily reversible by these agents. it has now been shown that the diff ... | 1979 | 390086 |
| bacterial contamination of expressed breast milk. | in a study of breast milk collected into sterile bottles rinsed in 1% hypochlorite solution the hypochlorite solution adherent to the sides of the bottles apparently caused a large reduction in bacterial contamination of the milk after storage at 4 degrees c for up to four hours. heating expressed breast milk at 62.5 degrees c for five minutes destroyed over 90% of the escherichia coli, staphylococcus aureus, and group b beta-haemolytic streptococci inoculated into the milk samples. rinsing coll ... | 1979 | 391343 |
| localization of the third component of complement on the cell wall of encapsulated staphylococcus aureus m: implications for the mechanism of resistance to phagocytosis. | encapsulated staphylococcus aureus strains are more virulent than unencapsulated staphylococci, and this phenomenon has been associated with decreased opsonization of encapsulated bacteria by normal human serum. peptidoglycan, a major cell wall component of s. aureus, has been shown to promote opsonization of this bacterial species by certain components of the serum complement system. however, when the processes of complement activation and opsonization of encapsulated staphylococci have been st ... | 1979 | 393630 |
| comparative bactericidal effect of ceforanide (bl-s 786) and five other cephalosporins in an in vitro pharmacokinetic model. | the bactericidal activity of ceforanide was compared, in an in vitro kinetic model, with that of five other cephalosproins: cephalothin, cefazolin, cefamandole, cefuroxime, and cefoxitin. cultures of various pathogens in 95% human serum were incubated for 12 hours in the presence of the cephalosporins whose concentrations were modified periodically-by addition of a concentrated solution of drug or dilution with unmedicated serum-in order to simulate the variation of antibiotic concentration in h ... | 1979 | 396292 |
| serotyping of serratia marcescens: simplified tube o-agglutination test and comparison with other serological procedures. | a simplified tube o-agglutination test was developed and evaluated for the determination of somatic serogroup (o) antigens of serratia marcescens. use was made of tryptic soy broth (tsb)-grown o-cells that had been boiled for 1 hour; 0.145 m nacl proved a satisfactory diluent. various technical parameters of this test were examined as well. rabbit anti-o immune sera, that had been elicited with 5 x concentrated, tsb-grown, 1 hour-boiled o-cells of all 15 currently employed o-antigen reference st ... | 1979 | 396741 |
| [staphylococcus aureus infections in domestic fowls. 1. ecology and epizootiology. (review)]. | the ecological and epizootiological aspects relating to staphylococcus aureus in domestic fowl are expounded, on the basis of literature, with particular reference being made to five topics: occurrence of these conditionally pathogenic bacteria in domestic fowl, point of attack and routes of infection, conditions for possible occurrence of infections, reciprocal infection between man and animal, as well as aspects of therapy and prophylaxis. | 1979 | 399432 |
| enumeration and identification of human leukemic lymphocytes by their natural binding of bacteria. | the recently described property of bacteria to bind to human lymphocytes was used to distinguish between normal and chronic leukemic lymphocyte (cll) populations. strains of the following bacteria were used in this study: arizona hinshawii, escherichia coli strains 1 and 2, bacillus globigii, brucella melitensis, corynebacterium diphtheriae strains 1 and 2, corynebacterium xerosis, sarcina lutea, staphylococcus aureus, and staphylococcus epidermidis. for identification of immunoglobulin-bearing ... | 1977 | 404035 |
| antimicrobial systems of the surgical wound. ii. detection of antimicrobial protein in cell-free wound fluid. | human wound fluid contains heat-stable proteins with moderate antibacterial activity against staphylococcus aureus and escherichia coli, and different heat-labile proteins with antibacterial activity against e coli. blood serum also contains heat-labile antibacterial substances, but little heat-stable activity against staphylococcus aureus. both blood serum and wound fluid have bacteriostatic activity against s epidermidis, and early growth of streptococcus fecalis occurs in serum and wound flui ... | 1977 | 404931 |
| heterogeneity of nonimmune immunoglobulin fc reactivity among gram-positive cocci: description of three major types of receptors for human immunoglobulin g. | two hundred and thirty strains of various gram-positive cocci were tested for quantitative, nonimmune binding of radiolabeled human polyclonal immunoglobulin g (igg). the majority of coagulase-positive staphylococci and streptococci belonging to serogroups c and g showed a high uptake of igg. the binding of immunoglobulin to group a streptococci was considerably less, with a number of strains completely negative. none of the pneumococcal or the group b or d streptococcal strains displayed any bi ... | 1977 | 409673 |
| [diminution of the antibacterial activity of antibiotics in cultures and in experimental mixed infections]. | we have studied interactions between staphylococcus aureus and pseudomonas aeruginosa in the absence and the presence of four different antimicrobials in mixed cultures and experimental infections. these two bacterial species, in addition to having different properties, are known to be opportunistic pathogens often present in human microflora. two main aspects have been investigated and they are related to modifications in two species affecting their equilibrium in the mixed bacterial population ... | 1978 | 417782 |
| cryptic peptidoglycan and the antiphagocytic effect of the staphylococcus aureus capsule: model for the antiphagocytic effect of bacterial cell surface polymers. | the antiphagocytic effect of the staphylococcus aureus capsule is known to be related to its ability to interfere with opsonization by normal human serum. in this study, evidence is presented with isolated cell surface components which indicates that the capsule hinders opsonization by masking cell wall peptidoglycan. in contrast to intact, encapsulated s. aureus m cells, peptidoglycan particles isolated from the organism were efficiently opsonized by normal human serum and phagocytized by human ... | 1979 | 422250 |
| beneficial effect of granulocyte transfusions in patients with defects in granulocyte function and severe infections. | a 3-year-old boy (patient a) with a congenital and a 24-year-old man (patient b) with an acquired granulocyte function defect received supportive granulocyte transfusions for the management of severe infections. the boy had suffered from recurrent infections since bith. his granulocytes showed in vitro almost no chemotactic responsiveness, an impaired phagocytosis and reduced intracellular killing of candida albicans. family studies suggested that it was an inherited autosomal recessive defect. ... | 1979 | 424696 |
| enterotoxin production by staphylococcus aureus strains isolated from cases of chronic osteomyelitis. | of 264 staphylococcus aureus strains selected at random from 3,978 strains isolated from chronic osteomyelitis patients, 79 were found to produce enterotoxin. a majority of the strains (65%) were nontypable by phages belonging to the international phage set for human strain typing. the significance of these strains and their circulation among the population is discussed, especially from the standpoint of their possible role in the development of osteomyelitis. | 1979 | 429545 |
| the function of human alveolar macrophages. | | 1979 | 430467 |
| double-antibody method and the protein-a-bearing staphylococcus aureus cells method compared for separating bound and free antigen in radioimmunoassay. | we compared the protein-a-bearing staphylococcus aureus immunoadsorbent to the double-antibody precipitation method for separating bound and free radiolabeled antigen in a radioimmunoassay. with human albumin (antigen) and rabbit anti-human albumin (antibody) as a model, our results indicate that formalin-fixed, heat-killed s. aureus cells could be substituted for the double-antibody precipitation method. ease of preparation and high adsorption capacity of protein-a-bearing s. aureus for most ma ... | 1979 | 436246 |
| binding of immunoglobulin g aggregates and immune complexes in human sera to staphylococci containing protein a. | using the cowan i strain of staphylococcus aureus, we compared the binding properties of human monomeric immunoglobulin (ig)g and oligomeric or complexed igg. heat-aggregated igg served as a model for complexed igg and heat-killed, formalin-fixed s. aureus (stapha) as a cellular receptor for igg, in determining the parameters for oligomeric and monomeric binding. because of its capacity for multipoint attachment, complexed igg binding was favored over monomeric igg binding, and this preferential ... | 1979 | 438327 |
| circulating staphylococcal antigen in humans and immune rabbits with endocarditis due to staphylococcus aureus: inhibition of detection by preexisting antibodies. | a radioimmunoassay for staphylococcal antigen that had detected antigenemia in each of 12 nonimmune rabbits with staphylococcal endocarditis detected antigen in sera from one of nine humans and two of eight immune rabbits with staphylococcus aureus endocarditis. staphylococcal antigens could be detected at concentrations as low as 0.78 microgram/ml when diluted in normal rabbit serum, compared with 6.25 microgram/ml when diluted in normal human serum and greater than 25 microgram/ml when diluted ... | 1979 | 458201 |
| [maternal lactation. ii. intestinal colonization in breast fed newborn infants]. | twenty-three low birth weight infants were studied to establish the role that breast milk plays in the intestinal colonization of the preterm infant, and in the control of epidemic diarrhea due to enteropathogenic e. coli, in nurseries. twelve of these case were fed breast milk in bottles, and eleven with "humanized" powder cow's milk (nan m.r.). bacteriologic studies of feces were performed daily during the first 18 days of life. in 5 cases given breast milk, and in 8 receiving cow's milk, stap ... | 1979 | 465174 |
| radioimmunoassay of glycosphingolipids: application for the detection of forssman glycolipid in tissue extracts and cell membranes. | development of a radioimmunoassay for detecting glycosphingolipids has been difficult, primarily because of transfer of radiolabeled glycolipid antigen to the unlabeled antigen pool. this difficulty has been overcome by the use of a radiolabeled glycolipid-polymer. thus, an assay system has been developed for measuring picomolar quantities of forssman hapten glycolipid. this assay is based on competition for rabbit anti-forssman antibodies between forssman glycolipid and a radiolabeled forssman ... | 1979 | 469271 |
| passive protection by human serum in mice infected with encapsulated staphylococcus aureus. | the occurrence and nature of passive protective antibody in 100 samples of human serum was investigated in mice challenged with strains of staphylococcus aureus capsular types a (smith diffuse strain) and b (strain ns58d). sixty of the sera passively protected mice against the capsular type-a strain, three against type b, and one against both types. rabbit antisera against human igg, iga and igm could remove the protective activity from a human serum of high potency, and the activity was also se ... | 1979 | 469929 |
| staphylococcal septic arthritis in three horses. | three horses were diagnosed as having monarticular septic arthritis due to staphylococcus aureus on the basis of culture of articular cartilage, synovial membrane and/or synovial fluid. the organisms were all well recognised human phage types and in two cases demonstrated beta-lactamase (penicillinase) activity. details of case histories are presented and the bacteriological techniques and antibiotic management with cloxacillin, methicillin and penicillin discussed. following treatment, sterile ... | 1979 | 477650 |
| proteolysis of human igg by human polymorphonuclear leucocyte elastase produces an fc fragment with in vitro biological activity. | the fc fragment derived by proteolysis of human igg by human polymorphonuclear leucocyte (pmn) elastase was tested for in vitro biological activity. this fragment could attach to the specific igg receptor of staphylococcus aureus cowan i cells (protein a) and could be eluted from the cells with dissociating buffer. taking advantage of this attachment, it was shown that the fc fragment is capable of attaching to the antigen-combining site of an igm rheumatoid factor and can bind to the fc recepto ... | 1979 | 487653 |
| effects of staphylococcus aureus lysozyme on human fibroblasts. | the purified lysozyme excreted by staphylococcus aureus strains promotes elongation and spreading on plastic surfaces and stimulates dna synthesis of human fibroblasts (wi38). this enzyme also raises twofold the saturation density level of cultures of these cells. it is suggested that the primitive and main effect of lysozyme on fibroblasts is the triggering of morphogenesis. | 1979 | 498287 |
| significance of serum factors in the stimulation of human umbilical cord and fetal lymphocytes by soluble protein a from staphylococcus aureus. | | 1979 | 499329 |
| evaluation of human monocyte function in vitro. | mononuclear phagocytes play a role in immunologic phenomena as well as in defence reactions of the organism. it is therefore important that clinicians have possibilities of evaluating their functions. a combination of simple and reproducible methods was set up to make this evaluation possible. the tests described in this paper are performed using peripheral blood monocytes and include: (1) phagocytic capacity of staphylococcus aureus and 51cr-labelled sheep erythrocytes, (2) bactericidal capacit ... | 1979 | 525978 |
| [bacterial antagonism of oral secretions towards staphylococci]. | bacterial antagonism may be one of the mechanism which regulates the bacterial flora of the pharynx. we have investigated the bacterial antagonism exerted in oral secretion by alpha-hemolytic streptococci against s. aureus and s. epidermidis. we cultured viridans streptococci and staphylococci in human saliva and we noted a bactericidal activity towards staphylococci. such activity, referable to the salivary system (peroxidase-thiocyanate) activated by hydrogen peroxide produced by streptococci, ... | 1979 | 553513 |
| [use of staphylococcal protein a for the detection of rubella virus igm antibodies]. | the ability of staphylococcus aureus protein a to bind human serum immunoglobulin of different classes and the conditions which allow a selective reduction of igg in comparison to other immunoglobulin classes were preliminarly studied. working under standardized conditions, a recovery of 3.5% of the initial value for igg, of 40-50% for igm and of 70-75% for iga was obtained. rubella hi antibodies, before and after staphylococcus treatment, were titred in 88 sera collected from adult women at dif ... | 1979 | 553518 |
| [cefamandole: in vitro and in vivo activity]. | among the cephalosporin antibiotics, a relatively new derivative of cefamandole has been studied, the sodium salt of its formyl ester, cefamandole nafate. it has shown a broad spectrum of antibiotic activity against a wide variety of gram-positive and gram-negative bacteria (127 strains); the susceptibility was good even with beta-lactamase-producing bacteria. good protective doses (pd50) were found after mice infections with staphylococcus aureus and salmonella wien. the pharmacokinetics of cef ... | 1979 | 553842 |
| a preliminary research on nutritional requirements in defined media to differentiate human staphylococci strains. | | 1979 | 554137 |
| radioimmunoassay for quantitation of antibodies to alphaviruses with staphylococcal protein a. | a radioimmunoassay (ria) procedure is described for measuring antibodies to alphaviruses in human and other mammalian sera. the test employed protein abearing staphylococcus aureus as a solid-phase immunoadsorbent for (3)h-labeled viruses complexed with immunoglobulin g. using antibodies produced in humans and guinea pigs, the ria procedure clearly differentiated among antibodies to venezuelan, western, and eastern equine encephalomyelitis viruses. sensitivity of the ria depended on the concentr ... | 1978 | 566768 |
| detection of antiplatelet antibody in immune thrombocytopenic purpura. | two methods have been developed for the detection of antiplatelet antibodies in immune thrombocytopenic purpura (itp). the first, a competitive ria, is highly sensitive, reproducible and allows quantitation of platelet associated immunoglobulins (pai) and specifically adsorbed antiplatelet antibodies from serum of patients with itp. the second procedure developed is a simple and rapid qualitative test, where antiplatelet antibodies are detected through the formation of rosettes of protein a prod ... | 1978 | 569356 |
| ecology of staphylococcus aureus: comparative characterization of strains isolated from man, cattle and sheep in bulgaria and in the gdr. | staphylococcus aureus strains from man, cattle and sheep have been differentiated by biochemical tests and by phage typing. strains from pathological material of human origin mostly belong to the host-specific variety hominis, strains from mastitis in cattle mostly belong to the host-specific variety bovis. however, there are also strains from cattle which cannot be allotted to one of the known host-specific varieties and also strains which belong to the host-specific variety hominis. strains fr ... | 1978 | 570580 |
| mycotic aneurysms of the hepatic artery. treatment with arterial embolization. | in a 29 year old white man with acute staphylococcus aureus endocarditis of the aortic valve two mycotic aneurysms of the hepatic artery developed during antibiotic treatment. one aneurysm was treated with surgical ligation and the other with gelfoam embolization. successful obliteration of the embolized aneurysm was demonstrated by hepatic arteriography two months later. the pathogenesis, clinical manifestations and management of hepatic mycotic aneurysms is discussed with emphasis on arterial ... | 1979 | 582881 |
| inactivation of penicillin by purulent exudates. | four of 22 specimens of human pus inactivated up to 90% of added penicillin within one hour in vitro. ampicillin and cephaloridine were also inactivated, but streptomycin and fusidic acid were not. the effect was not related to the protein content of the pus, nor to its ph value. microbes that may produce beta-lactamase in small quantities were isolated from three of the four specimens, but the enzyme was not detected in the pus by physical methods nor by microbiological inhibition assay. the in ... | 1977 | 585339 |
| aerobic bacterial flora of oral and nasal fluids of canines with reference to bacteria associated with bites. | oral and nasal fluids of 50 dogs were examined to determine the prevalence of aerobic bacteria frequently associated with animal bite wounds. the most frequently isolated microorganisms included: iij, ef-4, pasteurella multocida, staphylococcus aureus, staphylococcus epidermidis, group d streptococci, corynebacterium sp., enterobacteria, neisseria sp., moraxella sp., and bacillus sp. other species and genera were infrequently recovered and may represent transient flora. the high incidence of iij ... | 1978 | 632349 |
| phagocytosis and killing of staphylococci by human polymorphonuclear and mononuclear leucocytes. | the phagocytosis and killing of 3h-thymidine-labelled staphylococcus aureus by polymorphonuclear leucocytes (pmns) and monocytes (mns) obtained from 50 health donors were evaluated. in addition, extracellular factors that might influence phagocytosis and killing were studied. the method described gave highly reproducible results. no significant difference was observed in the phagocytic and killing functions of a single donor's pmns and mns when studied several times in one day and longitudinally ... | 1978 | 670409 |
| opsonins in human serum for the phagocytosis of complexes between igg and protein a of staphylococcus aureus. | the phagocytosis of complexes between staphylococcal protein a and 125i-labelled igg is enhanced by fresh human serum and to a somewhat lesser extent by heat-inactivated serum. opsonizing activity is detected in two distinct peaks by sephadex g-200 column chromatography. one peak corresponds to the excluded material and the other to the ascending limb of the second protein peak. the active material in serum is retained on a protein a-sepharose but not on an igg-sepharose column. active material ... | 1978 | 680797 |