Publications

asbestos-induced chemiluminescence response of human polymorphonuclear leukocytes.	products of the oxidative metabolism of polymorphonuclear leukocytes (pmn), namely hydrogen peroxide and superoxide, may play a role in tissue injury at sites of inflammation. oxidant production by pmn was measured by the luminol-enhanced chemiluminescence (cl) of cells exposed in vitro to asbestos fibers. human peripheral blood pmn were incubated with 1-1000 micrograms/ml of uicc chrysotile b asbestos fibers opsonized with either normal or heat-inactivated human serum. cl was measured over 210 ...	1984	6692804
advantages of sequential polyethylene glycol (peg) precipitations to isolate and analyze circulating immune complexes.	the present report describes a new approach utilizing sequential polyethylene glycol 6000 (peg) precipitations to isolate and analyze circulating immune complexes (ic). two experimental systems were studied. first, ic were formed by adding labeled or unlabeled bovine serum albumin (bsa) to anti-bsa antiserum. sequential precipitations with peg concentrations ranging from 2 to 6.2 indicated that immunoglobulin (ig) molecules were precipitated in all experimental and control sera tested. in contra ...	1984	6706381
the multiplicity of human pancreatic secretory trypsin inhibitor.	four forms of pancreatic secretory trypsin inhibitor (psti; a1, a2, b, and c) were purified from human pancreatic juice. according to sequence results, the primary structure of b was different from that reported earlier (greene, l.j., et al. (1976) method enzymol. 45, 813-825) at two positions, i.e. asn21----asp21, asp29----asn29. a1 and a2 were deamidated forms of b judging from peptide mappings with staphylococcus aureus v8 protease. gln45 in b was replaced by glu in a1 and gln51 in b was repl ...	1985	4086468
hormone-dependent covalent modification and processing of human progesterone receptors in the nucleus.	in situ photoaffinity labeling, which minimizes in vitro incubations and proteolytic artifacts, was used to study the structure of progesterone receptors (pr) in intact t47d human breast cancer cells. these cells, rich in pr, were incubated with the photoreactive progestin [3h]r5020 at 0 degrees c for 3 hr to keep pr in their untransformed state, or at 37 degrees c for 5 min to transform pr and convert them to tight chromatin-binding proteins. the cells, still intact, were then irradiated with 3 ...	1985	4092571
bacterial adherence to human endothelial cells in vitro.	differences in the ability of bacteria to adhere to normal valvular endothelium may account for the predominance of particular species as pathogens in acute endocarditis. an in vitro adherence assay was developed to simulate the host surface encountered in acute bacterial endocarditis by using confluent monolayers of human endothelial cells. adherence of 32 gram-positive and -negative blood culture isolates to this surface was compared. all five staphylococcus aureus strains tested were highly a ...	1985	4044035
relationship between extracellular stimulation of intracellular killing and oxygen-dependent microbicidal systems of monocytes.	human monocytes require serum components immunoglobulin g, c3/c3b, and b/bb to exert optimal microbicidal action against ingested microorganisms. the present study was performed to find out whether these factors act by enhancing oxygen-dependent antimicrobial mechanisms. serum enhanced oxygen consumption and superoxide production by monocytes before phagocytosis, but did not further increase these processes in monocytes that had recently ingested bacteria. furthermore, serum did not boost iodina ...	1985	2981774
catalytic properties of a human cytomegalovirus-induced protein kinase.	human cytomegalovirus, a dna virus whose genome contains a fragment of transforming dna, induces a threonine-serine protein kinase having a molecular mass of 68 kda (p68). p68 was extracted from cells 96-144 h after infection, and immunoprecipitated with a monoclonal antibody (f6b). antibody-enzyme complexes were immobilized on heat/formaldehyde-inactivated staphylococcus aureus. the best substrates for p68 were acidic proteins, phosvitin and casein. glycogen synthase, phosphorylase alpha and hi ...	1985	2986975
permeabilization of rat hepatocytes with staphylococcus aureus alpha-toxin.	pathogenic staphylococci secrete a number of exotoxins, including alpha-toxin. alpha-toxin induces lysis of erythrocytes and liposomes when its 3s protein monomers associate with the lipid bilayer and form a hexomeric transmembrane channel 3 nm in diameter. we have used alpha-toxin to render rat hepatocytes 93-100% permeable to trypan blue with a lactate dehydrogenase leakage less than or equal to 22%. treatment conditions included incubation for 5-10 min at 37 degrees c and ph 7.0 with an alpha ...	1985	2987273
effect of ciprofloxacin on phagocytosis.	certain aspects of the relationship between host defence mechanisms and the new quinoline derivative ciprofloxacin in comparison to norfloxacin and ofloxacin were studied. ciprofloxacin did not affect chemotaxis of human polymorphonuclear leucocytes in agarose. in leucocytes exposed to ciprofloxacin, norfloxacin and ofloxacin neither the chemiluminescent response to opsonized zymosan and formyl-methionyl-leucyl-phenylalanine nor the phagocytic or bactericidal activity was affected. however, kill ...	1985	2936604
deposition of c3b and ic3b onto particulate activators of the human complement system. quantitation with monoclonal antibodies to human c3.	monoclonal antibodies were used to determine the number and molecular form of c3 bound to particulate activators of the complement (c) system by human serum. sheep erythrocytes (e) coated with igm (eigm) and igg (eigg) were used to study activation of the classical pathway (cp). yeast (y), rabbit erythrocytes (er), and five species of bacteria (escherichia coli, staphylococcus aureus, streptococcus pneumoniae type 3, streptococcus pyogenes, and hemophilus influenzae type b) were used to study ac ...	1985	2409200
immunological uniqueness of human monoamine oxidases a and b: new evidence from studies with monoclonal antibodies to human monoamine oxidase a.	monoamine oxidase (ec 1.4.3.4; mao) is the primary enzyme responsible for the intraneuronal degradation of biogenic amines in the central nervous system. an understanding of the physiological significance of the functional and regulatory differences between the two forms of the enzyme, maos a and b, would be facilitated by the availability of antibodies specific for the two forms of the enzyme. we previously isolated and characterized a monoclonal antibody (mao b-1c2, previously designated mao-1 ...	1985	2414414
antibodies to lipoteichoic acid from staphylococcus aureus. specificity of murine monoclonal and human antibodies.	two monoclonal antibodies against staphylococcal lipoteichoic acid (lta) were made by fusing p3x63ag8 myeloma cells and splenocytes from mice immunized with purified lta. both were isotyped as being igm kappa. their specificities were determined by enzyme-linked immunosorbent assays indicating that both antibodies reacted with the glycerol-phosphate backbone, while one of them also had some affinity for the alanyl substituent. antibodies in serum from 7 multiple sclerosis (ms) patients and serum ...	1985	2424261
regulation of human ige response by t cells and their products.	an in vitro experimental system for the induction of human ige production has been established with human peripheral blood lymphocytes (pbl). for the assessment of ige produced in vitro, a sensitive elisa method has been developed by employing monoclonal anti-ige antibodies. the stimulation of pbl with pokeweed mitogen plus staphylococcus aureus strain cowan i induced polyclonal ige response. t cells from patients with pulmonary tuberculosis, after activation with purified protein derivative and ...	1985	2409024
specific and cross-reacting antigens of staphylococcus aureus of human and canine origins.	biotype -specificity of staphylococcus aureus of human and canine origins has been found to be associated with thermolabile agglutinogens represented in s. aureus strains 17 and 61218, respectively. both strains also have exhibited a common thermostable antigen. on that basis, absorbed antisera have been developed for the differentiation of s. aureus of the two biotypes. in the present study, still another thermostable agglutinogen was established, shared by strain 17 and some s. aureus strains ...	1985	2578480
synergistic hemolysis exhibited by species of staphylococci.	the synergistic hemolysis reactions of 61 reference strains and 189 clinical isolates representing 17 species of staphylococci were examined on plates of trypticase soy blood agar (bbl microbiology systems, cockeysville, md.). some or all of the strains of staphylococcus aureus, s. epidermidis, s. capitis, s. cohnii, s. haemolyticus, s. hyicus, s. simulans, s. warneri, and s. xylosus produced a delta-hemolysin that gave synergistic, complete hemolysis of washed human, sheep, and ox blood cells i ...	1985	4044799
the expression of capsule in serum-soft agar by staphylococcus aureus isolated from human clinical sources.	staphylococcus aureus isolates from human clinical sources were incubated for various times in modified 110 medium and tested for production of capsule by the serum-soft agar technique. ten (5.7%) of 175 isolates were encapsulated after incubation for 24 h. a more detailed examination of 77 isolates showed that incubation period affected the production of capsule. after 2 h, 31% were encapsulated, but after 6 h and 24 h this decreased to 17% and 4% respectively. rapid passage in vitro induced th ...	1985	4045993
[hemolytic effect of staphylotoxin].	low concentrations of staphylotoxin hemolyzed human erythrocytes at 37 degrees following the kinetics of the first order, high concentrations--by means of kinetics of the second order. effect of the toxin was distinctly augmented with increase of temperature above 20 degrees. treatment of erythrocytes with proteolytic enzymes elevated the cell sensitivity to the staphylotoxin. liposomes, containing a mixture of lecithin, cholesterol and/or hydrocortisone as well as the total lipid fraction from ...	1985	3911574
in vitro ige-secreting cells in man. i. mitogen-independent and -dependent subpopulations.	in vitro ige secretion by atopic and normal peripheral-blood lymphocytes was examined in culture with pokeweed mitogen or staphylococcus aureus strain cowan-i (stacw) or without mitogen. ige secreted in culture supernatants was measured with double antibody radioimmunoassay. enumeration of ige-secreting cells was made by a protein-a plaque assay. ige was detected in increasing quantities in supernatants of cultured lymphocytes without mitogen up to the 12th day. ige-plaque-forming cells were for ...	1985	4038923
recognition and management of acute suppurative parotitis.	acute suppurative parotitis (asp) is a rare infectious process occurring after surgery or in debilitated patients. this case describes a 41-year-old man who was hospitalized initially for acute bacterial endocarditis. late in the hospital course his recovery was complicated by the development of asp. subsequent cultures of blood and parotid-fluid samples grew pseudomonas aeruginosa. despite early empiric antibiotic therapy followed by more specific therapy with an aminoglycoside, the patient con ...	1985	4053524
susceptibility of human and porcine neutrophils to hypothermia in vitro.	hypothermia may contribute to serious life-threatening infections. an experimental model has been established in pigs in order to study the effects of hypothermia on host bacterial defenses. the function of blood neutrophils from pigs and humans was examined in vitro at 37 and 29 degrees c. bacterial killing of staphylococcus aureus 502a by human neutrophils after 90 and 180 min incubation at 29 degrees c was reduced to 76 +/- 6% and 83 +/- 7% of killing at 37 degrees c. porcine neutrophil killi ...	1985	4058977
induction of human interleukin-1 by toxic-shock-syndrome toxin-1.	strains of staphylococcus aureus isolated from patients with toxic shock syndrome (tss) make a characteristic protein known as toxic-shock-syndrome toxin-1 (tsst-1), but the role of this protein in the pathogenesis of tss is not certain. we have purified tsst-1 by using a combination of alcohol precipitation, isoelectric focusing, and gel chromatography. tsst-1 has an isoelectric point of 7.2 and a molecular weight of 23,100, in accordance with previously published determinations for this protei ...	1985	3871826
detection of c-abl tyrosine kinase activity in vitro permits direct comparison of normal and altered abl gene products.	the v-abl transforming protein p160v-abl and the p210c-abl gene product of the translocated c-abl gene in philadelphia chromosome-positive chronic myelogenous leukemia cells have tyrosine-specific protein kinase activity. under similar assay conditions the normal c-abl gene products, murine p150c-abl and human p145c-abl, lacked detectable kinase activity. reaction conditions were modified to identify conditions which would permit the detection of c-abl tyrosine kinase activity. it was found that ...	1985	3879812
determination of maximal bactericidal activity in human granulocytes.	a conventional in vitro test assay was used to determine maximal bactericidal capabilities of human granulocytes. by means of a mathematical model the maximal phagocytosis and killing activity could be calculated for s. aureus and p. aeruginosa serving as test organisms. the evaluation allowed moreover the determination of the optimal bacterial load and also of critical bacterial concentrations leading to a complete depression of observable granulocyte killing functions. in contrast to other stu ...	1985	3919784
suppression of lymphocyte proliferation by pseudomonas aeruginosa: mediation by pseudomonas-activated suppressor monocytes.	pseudomonas aeruginosa has been shown to suppress cell-mediated immunity in experimental animals, but recent reports have also demonstrated that there is a strong t-cell response to this bacteria. our studies of human peripheral blood mononuclear cells showed a great variation in the in vitro proliferative response to killed p. aeruginosa, so we examined the interaction of the different mononuclear cells in cultures with this bacteria. p. aeruginosa stimulated the proliferation of t lymphocytes, ...	1985	3922895
survival of gram-negative and gram-positive bacteria artificially applied on the hands.	we evaluated the survival of pseudomonas aeruginosa, klebsiella pneumoniae, serratia marcescens, escherichia coli, and staphylococcus aureus, derived from either hospitalized patients or culture collections, on the fingertips of human volunteers. over 99% of the bacteria died within 2 min of the application, and about 10(5) cells remained on the fingers for up to 90 min.	1985	3988907
bacteriological analysis of different foods to determine the fitness for human consumption.		1985	3923224
pharmacokinetics of macrolides, lincosamides and streptogramins.	macrolide antibiotics are known to be effective in spite of their low blood levels. this results in an exception to the customary rule of antibiotics evaluation, of judging the in-vivo effect of an antibiotic in terms of blood levels and mics. most efforts to improve blood levels of macrolides have been unsuccessful because of hepatic toxicity. intravenous administration of macrolides has been difficult because of the frequent incidence of severe side effects. in the present paper, the in-vivo d ...	1985	3932301
[digestive and cutaneous colonization of staphylococcus aureus, pseudomonas aeruginosa and corynebacterium xerosis in germ-free hairless mice].	regardless of the method of germ-free hrs mouse contamination, the kinetics of staphylococcus aureus and pseudomonas aeruginosa cutaneous colonization was parallel to the kinetics of intestinal colonization; corynebacterium xerosis, however, was unable to colonize the skin or the gastrointestinal tract. there was little variability in intestinal and cutaneous colonization; this was quite different from observations in man and hairless holoxenic mice. it is possible to use these experimental mode ...	1985	3936400
kinetics of il-2 and interferon-gamma production, expression of il-2 receptors, and cell proliferation in human mononuclear cells exposed to staphylococcal enterotoxin a.	staphylococcal enterotoxin a (sea) at picogram amounts induces high levels of interleukin 2 (il-2) and interferon in human mononuclear cells. sea is a stronger inducer of il-2 than phytohemagglutinin, leukoagglutinin, and concanavalin a. the il-2 induction is very rapid with maximal levels being reached after 18 to 24 hr. the il-2 concentration decreases rapidly and almost no il-2 activity can be detected in supernatants of cells cultured for 3 days or more. maximal dna synthesis is recorded 3 d ...	1985	3939109
role of fibronectin in human monocyte and macrophage bactericidal activity.	fibronectin is a high-molecular-weight glycoprotein found as a soluble dimer in plasma and as an insoluble multimer in tissues. it has been proposed that plasma fibronectin facilitates phagocytic removal of lysed cells and damaged tissues. fibronectin binds avidly to several species of gram-positive bacteria and enhances staphylococcal and streptococcal attachment to cultured cells. determination of whether fibronectin will enhance the bactericidal activity of monocytes and macrophages has not b ...	1985	3972444
neutralization of erythroid burst-promoting activity in vitro with antimembrane antibodies.	to investigate the relatedness of soluble and pelletable vesicular erythroid burst-promoting activity (bpa) present in lymphocyte-conditioned medium (lcm), we immunized rabbits with partially purified lymphocyte plasma membranes and tested the antisera for biological and immunologic crossreactivity with lcm and its component fractions. when preincubated with igg purified from post-immune but not from preimmune serum, bpa expression by unseparated lcm, lcm-derived pellets, and supernatants was ab ...	1985	3978232
the cross-linking of human met-hemoglobin with [14c]dimethyl adipimidate.	met-hemoglobin, cross-linked with [14c]dimethyladipimidate (cross-linking span 9 a; 1 a = 0.1 nm), yields four distinct molecular weight products (monomer, dimer, trimer, and tetramer) as observed on sodium dodecyl sulfate - polyacrylamide gels. the dimer species was purified to homogeneity by gel filtration. it was proteolytically degraded using a combination of staphylococcus aureus v8 protease, pepsin, trypsin, and chymotrypsin. the resultant peptides were fractionated using gel filtration an ...	1985	3986666
effects of in vitro corticosteroids on b cell activation, proliferation, and differentiation.	the present study demonstrates the graded effect of in vitro corticosteroids (css) on the different phases of b cell activation, proliferation, and differentiation. early events such as activation and proliferation of high-dose anti-mu or staphylococcus aureus-stimulated b cells are profoundly suppressed by the presence of in vitro css. the suppressed proliferative response may be mediated by a direct effect on b cells and/or modulation of accessory cell function. later events in the b cell cycl ...	1985	3871795
in vitro translation of mrna from toxocara canis larvae.	300 micrograms of total rna was extracted from 1 ml of packed toxocara canis larvae by centrifugation through a 5.7 m cesium chloride cushion. 60 micrograms of polyadenylated messenger rna was separated from 300 micrograms of total rna in an oligothymidylic acid-cellulose gel column. the in vitro translation of the mrna, isolated from t. canis larvae, was carried out using the rabbit reticulocyte cell-free translation system. incorporation of [35s]methionine into trichloroacetic acid precipitabl ...	1985	3990707
serological characteristics of coagulase-positive staphylococci isolated from man and animals.	a simplified method allowed staphylococcus aureus, staph. intermedius and coagulase-positive staph. hyicus subsp. hyicus isolated from humans, dogs, monkey, sheep, poultry, rabbits, giant rats (cricetomys gambianus) and other animals to be serotyped. the nine coagulase-positive staphylococcal strains of human origin possessed thermolabile and thermostable agglutinogens. two strains of staph. intermedius of human and canine origins examined had agglutinogen k1k2. the three staph. aureus strains i ...	1985	3997688
cellular and bacterial toxicities of topical antimicrobials.	cellular and bacterial toxicities of four commonly used topical antimicrobials (1% povidone-iodine, 3% hydrogen peroxide, 0.25% acetic acid, and 0.5% sodium hypochlorite) were assayed in vitro using cultures of human fibroblasts and staphylococcus aureus. all agents tested at full strength killed 100 percent of exposed fibroblasts. fibroblast toxicity exceeded bacterial toxicity with serial dilutions of hydrogen peroxide and acetic acid. dilutions of povidone-iodine (1:1000) and sodium hypochlor ...	1985	3975287
adherence of lysostaphin to and penetration into human monocytes.	the effect of lysostaphin on staphylococcus aureus phagocytosed by monocytes was investigated. the results showed that lysostaphin adheres to monocytes by a temperature-independent mechanism, is not adequately removed from monocytes by washing, and penetrates by means of a temperature-dependent mechanism. in in vitro assays of monocyte function, phagocytosed s. aureus can be killed by lysostaphin after penetration of the cells during incubation or by adhering lysostaphin when the monocytes are d ...	1985	3975574
in vitro activity of ci-934, a quinolone carboxylic acid active against gram-positive and -negative bacteria.	ci-934 is a totally synthetic difluorinated quinolinecarboxylic acid with an ethyl-amino-methyl pyrrolidine side chain, which has broad-spectrum antibacterial activity, including particular potency directed against streptococci and staphylococci. the ci-934 mic (micrograms per milliliter) for 90% of the strains tested was 0.4 (range, 0.2 to 0.8) for a group of streptococci (pneumococci, viridans streptococci, streptococcus faecalis, and lancefield groups a, b, and c), 0.2 (0.05 to 0.2) for staph ...	1985	3866513
comparison of a new cephalosporin, bmy 28142, with other broad-spectrum beta-lactam antibiotics.	bmy 28142, a new broad-spectrum semisynthetic cephalosporin, was evaluated in vitro and in vivo in comparison with ceftazidime, cefotaxime, moxalactam, and cefoperazone. the activity of bmy 28142 compared favorably with the activities of the other compounds against both pseudomonas aeruginosa and staphylococcus aureus and was somewhat greater against members of the family enterobacteriaceae. the influence of inoculum size on mics of bmy 28142 was small for most of the isolates tested, except ent ...	1985	3885849
distribution of the major histocompatibility complex antigens in human and rat kidney.	we have compared the distribution of the major histocompatibility complex (mhc) antigens in human and rat kidney using monospecific antisera to class i and ii antigens of the mhc. fitc/tritc double immunofluorescence was used to demonstrate these antigens in frozen sections and the staphylococcus aureus cowan i rosette assay on the cell surface. in both species, the mhc antigens were prominently present on the passenger leukocytes. immunofluorescence analysis of human kidney demonstrated that th ...	1985	3892132
comparative in-vitro activity of sch 34343, a new penem antibiotic.	using an agar dilution technique we examined the in-vitro activity of sch 34343 against 485 clinical bacterial isolates. ampicillin, mezlocillin, cefuroxime, cefotaxime, cefotetan, ceftazidime, ceftriaxone, imipenem (n-formimidoyl thienamycin) and gentamicin were used for comparison. sch 34343 exhibited activity against all species tested, excepting pseudomonas aeruginosa and pseudomonas species. unlike many newer beta-lactams, sch 34343 was highly active against gram-positive species. it was co ...	1985	3849538
activation of neutrophils by antigen-induced lymphokine, with emphasis on antibody-independent cytotoxicity.	incubation of bovine neutrophils with antigen-stimulated mononuclear cell supernatant (lymphokine) caused an inhibition of neutrophil migration and an enhancement of the neutrophils' ability to adhere to plastic, reduce nitroblue tetrazolium, ingest staphylococcus aureus, and mediate antibody-dependent cell-mediated cytotoxicity (adcc) against chicken erythrocytes. lymphokine-treated neutrophils also became cytotoxic for chicken, turkey and human erythrocytes in the absence of specific antibody ...	1985	3862726
immunoglobulins in the hyperimmunoglobulin e and recurrent infection (job's) syndrome. deficiency of anti-staphylococcus aureus immunoglobulin a.	patients with the hyperimmunoglobulin e and recurrent infection syndrome (hie) characteristically have frequent skin and respiratory infections caused by staphylococcus aureus. we have developed a set of enzyme-linked immunosorbent assays that use whole s. aureus (wood's strain) immobilized on 0.22-micrometers filters and highly specific, affinity-purified enzyme conjugates of goat anti-human ige, anti-human igd, anti-human igg, anti-human iga, and anti-human igm. these reagents were used to det ...	1985	3871199
[studies of the mechanisms of human b-cell activation. iv. abnormalities at the b-cell level in patients with behçet's disease].		1985	3875902
role of class ii histocompatibility antigens in staphylococcus aureus protein a-induced activation of human t lymphocytes.	the capacity of peripheral blood monocytes and b lymphocytes to support staphylococcal protein a (spa)-induced proliferation of autologous and allogeneic t cells, as well as the role of major histocompatibility complex (mhc) class i and ii molecules in this activation process, were investigated. highly purified peripheral t lymphocytes did not proliferate in response to spa, but their response was reconstituted by both irradiated (or mitomycin c-treated) monocytes and b lymphocytes. the effect o ...	1985	3871365
effects of diacetyl diamines on in vitro activation and proliferation of human b lymphocytes.	n,n'-diacetylputrescine (tetramethylenebisacetamide [tmba]) and its six carbon analog, hexamethylenebisacetamide (hmba), inhibited the proliferative response of human b lymphocytes to anti-mu and formalinized cowan i strain staphylococcal aureus (sac) stimulation. in contrast, b cell growth factor-stimulated proliferation of human b cells was minimally inhibited by tmba or hmba. the antiproliferative effect of these diamine derivatives was specific for anti-mu (or sac) activation of normal b cel ...	1985	3877754
b cell growth factor activity of immunoaffinity-purified and recombinant human interleukin 2.	we investigated the effect of recombinant and affinity-purified human interleukin 2 (il2) on human b cell proliferation. five x 10(4) nonadherent spleen cells that had been depleted twice of t cells were activated by 3-day culture with formaldehyde-killed staphylococcus aureus cowan strain i (sac) prior to addition of tested growth factors. cultures were harvested 72 h later. it was found that both il2 preparations led to optimal cell proliferation compared with a control supernatant obtained by ...	1985	3871701
interleukin 2-induced proliferation of leukemic human b cells.	the proliferative responses of purified leukemic human b cells from nine b cell chronic lymphocytic leukemias to recombinant interleukin 2 (il-2), spontaneously, and after preactivation by staphylococcus aureus cowan i (sac) or anti-mu antibodies were studied. three patterns of response were observed: (a) no response (three cases); (b) a moderate spontaneous response enhanced by anti-mu (one case); (c) a high proliferative response after preactivation by anti-mu and/or sac (five cases). il-2 cou ...	1985	3872922
colonization of human wounds by escherichia vulneris and escherichia hermannii.	in this report we present clinical descriptions of 12 hawaiian patients from whom escherichia vulneris or e. hermannii strains were isolated. all but two patients had soft-tissue infections with multiple bacteria, particularly staphylococcus aureus. the other two had purulent conjunctivitis associated with s. aureus and infected malignant peritonitis with multiple organisms, respectively. in none of the cases were the escherichia spp. found in abundant quantities or considered pathogenic. in pre ...	1985	3897270
an enzyme immunoassay for the detection of staphylococcal protein a in affinity-purified products.	rabbit antiserum, specific for protein a from staphylococcus aureus, was conjugated to alkaline phosphatase and used in a double antibody solid-phase enzyme immunoassay. the assay was developed to monitor eluate from a large-scale protein a-sepharose affinity column used to purify monoclonal antibodies for human clinical trials. the assay detected soluble protein a in the presence of immunoglobulin at concentrations as low as 4 ng/ml. analysis of the product purified by affinity chromatography r ...	1985	3902970
the effects of hypothermia on neutrophil function in vitro.	hypothermia may be associated with compromised host defenses and serious bacterial infections in man. we have examined the effects of moderate hypothermia (29 degrees c) on neutrophil function in vitro. at 29 degrees c, neutrophil phagocytosis of staphylococcus aureus was impaired. in contrast, neutrophil killing of streptococcus faecalis was most affected by hypothermia. phagocytosis, as measured by neutrophil ingestion of opsonized oil-red-o-particles, was reduced at 29 degrees c over the 15 m ...	1985	3917485
effect of bacterial products on human ciliary function in vitro.	ciliary activity protects the respiratory tract against inhaled particles, including bacteria, by transporting them trapped in mucus towards the pharynx. we have studied the effect of bacteria (haemophilus influenzae, staphylococcus aureus, and pseudomonas aeruginosa) on human nasal cilia, measuring their in vitro ciliary beat frequency by a photometric technique. supernatant fluids were obtained from 18 hour broth cultures by centrifugation alone, by filtration, and by lysis. supernatants obtai ...	1985	3919460
the primary structure of rabbit and rat prealbumin and a comparison with the tertiary structure of human prealbumin.	the primary structures of rabbit and rat prealbumin have been determined. the amino acid sequence of rabbit prealbumin was determined by analyses of peptides obtained by trypsin and staphylococcus aureus protease digestions. the rat prealbumin sequence was deduced by analyses of tryptic peptides as well as by nucleotide sequencing of cdna clones. both amino acid sequences contain 127 amino acid residues, the same as human prealbumin. pairwise comparisons show that the three sequences are more th ...	1985	3922975
human mononuclear cells exposed to staphylococci rapidly produce an inhibitor of neutrophil chemotaxis.	serum-free cultures of human peripheral blood mononuclear cells from normal volunteers produce an inhibitor of neutrophil chemotaxis when exposed to heat-killed staphylococci. human neutrophils were exposed to 100-fold dilutions of supernatants from 6-hr cultures, washed repeatedly, and assayed for chemotactic responsiveness with a radiolabel assay. dilutions of supernatants from cell cultures exposed to staphylococci resulted in a mean chemotaxis of 856 +/- 83 cpm (n = 21), while that for mediu ...	1985	3874251
regulation of human b cell activation by prostaglandin e2. suppression of the generation of immunoglobulin-secreting cells.	the role of prostaglandin e2 (pge2) in the generation of immunoglobulin-secreting cells (isc) from human peripheral blood b cells was examined. initial studies demonstrated that monocyte (m phi)-mediated suppression of the generation of isc in staphylococcus aureus (sa)-stimulated cultures was mitigated by indomethacin, and thus suggested that the cyclooxygenase pathway products of arachidonic acid played a role in the regulation of b cell activation. the possibility that pge2, one of the major ...	1985	3872889
cathepsin d precursors in clathrin-coated organelles from human fibroblasts.	coated vesicles were isolated from metabolically labeled human fibroblasts with the aid of affinity-purified antibodies against human brain clathrin and staphylococcus aureus cells. the material adsorbed to the s. aureus cells was enriched in clathrin. when the s. aureus cells bearing the immunoadsorbed material were treated with 0.5% saponin, extracts containing the precursor form of cathepsin d were obtained. the extraction of the precursor was promoted in the presence of mannose 6-phosphate. ...	1985	3928634
bactericidal effects of photoradiation therapy with hematoporphyrin derivative.	hematoporphyrin derivative (hpd) localizes selectively in malignant and rapidly metabolizing tissues and undergoes a cytotoxic reaction when exposed to light of a specific wavelength. hpd has been studied extensively with regard to the diagnosis and treatment of tumors but not with regard to bactericidal activity. this investigation assessed the effect of light-activated hpd on various microorganisms, on human polymorphonuclear leukocytes, and on the interactions of polymorphonuclear leukocytes ...	1985	3155547
complexes prepared from protein a and human serum, igg, or fc gamma fragments: characterization by immunochemical analysis of ultracentrifugation fractions and studies on their interconversion.	protein a of staphylococcus aureus is an fc receptor for igg that has been used as a therapeutic reagent to treat cancer in humans and experimental animals. we used ultracentrifugation combined with analysis of isolated fractions by radioimmunoprecipitation and competitive radioimmunoassay with chicken antibodies that bind free protein a or protein a in complexes but do bind free immunoglobulin reagents to localize and characterize the types of complexes formed with different molar ratios of 125 ...	1985	3157047
recombinant interleukin 2 and gamma-interferon act synergistically on distinct steps of in vitro terminal human b cell maturation.	the effects of recombinant interleukin 2 (il-2) on the in vitro differentiation of human tonsillar b cells which were not preincubated with staphylococcus aureus cowan i or with anti-human igm were investigated. il-2 was shown to induce the generation of ig-containing cells in a dose-dependent fashion from 2.5 to 2,500 u il-2/ml. conversely, the quantities of ig secreted in the culture supernatant were found in the majority of experiments to peak at 25 u/ml. the possible presence, in cultures st ...	1986	3082936
effects of anti-igm on mitogen-induced proliferation of human b-lymphocyte malignancies.	therapeutic trials of anti-immunoglobulin antibody have produced a wide range of responses in attempts to control the growth of human b lymphoid neoplasms. this variability might reflect differences in intrinsic functional characteristics of malignant b lymphocytes that determine susceptibility to anti-immunoglobulin-mediated regulation of growth. to characterize b-lymphocyte malignancies, tissue samples from 24 patients were studied during short-term culture in vitro. malignant b lymphocytes we ...	1986	3084144
binding of chicken, bovine, and rabbit immunoglobulins by avian, bovine, and human strains of staphylococcus aureus.	sixty-six strains of staphylococcus aureus of avian, bovine, and human origin were tested for their ability to bind chicken, bovine, and rabbit immunoglobulin g (igg). a microtitration plate hemagglutination assay and a direct-tube enzyme immunoassay were used to determine qualitative differences. twice as many chicken and bovine s. aureus isolates than human strains reacted positively to chicken igg. mean binding values of chicken igg were also twice as high for chicken and bovine s. aureus iso ...	1986	2942849
opsonic activity of plasma fibronectin for staphylococcus aureus by human alveolar macrophages: inefficacy of trypsin-sensitive staphylococcal fibronectin receptor.	the binding of 125i-fibronectin (fn) to some bacteria and the phagocytic activity of human alveolar macrophages (am) by a culture method using 3h-labelled bacteria were examined. fn-binding of staphylococcus aureus (s. aureus) was higher than that of gram-negative rods and was reduced markedly by trypsin-treatment. fn enhanced the uptake of s. aureus by am in dose dependent manner. to determine whether fn-binding to s. aureus mediates the promotion of staphylococcal phagocytosis, we compared the ...	1986	2943056
immunomodulation of human leukocytes by staphylococcal enterotoxin a: augmentation of natural killer cells and induction of suppressor cells.	staphylococcal enterotoxin a (sea), a protein isolated from culture supernatants of staphylococcus aureus, is a potent t-cell mitogen and an inducer of interferon-gamma (ifn-gamma). we report here that sea exhibits a number of significant in vitro immunomodulatory functions. in vitro treatment of human peripheral blood monocyte-depleted lymphocytes with sea resulted in significant augmentation of their natural killer cytotoxicity against target cells from hemopoietic (k562, daudi) or solid (mela ...	1986	2943428
characteristics of staphylococci isolated from man, poultry and some other animals.	of 281 strains of staphylococci isolated from man and animals 36 (12.8%) were coagulase-positive and 245 (87.2%) were coagulase-negative. staphylococcus aureus and staph. intermedius were the commonest coagulase-positive staphylococci isolated from the hosts examined. of the 20 strains that remained unclassifiable, 14 were isolated from sheep and goats.	1986	3084438
expression and function of an early activation marker restricted to human b cells.	a b cell-specific monoclonal antibody (anti-ba) was prepared. in two-color facs analysis the anti-ba reacted with a subpopulation of ig+ or b1+ cells obtained from tonsils, but did not react with most b1+ cells derived from pbl. activation of b cells from pbl with tpa or anti-mu induced ba expression and the addition of pha-conditioned supernatant with anti-mu-enhanced ba expression. other b cell activators, such as staphylococcus aureus cowan i (staph-a) or pwm plus t cells, could induce ba exp ...	1986	3084651
effects of human serum on bacterial competition with neutrophils for molecular oxygen.	a dialyzable factor(s) in human serum is known to stimulate gonococcal oxygen consumption. its effect on other human pathogens was investigated. a 10% serum solution increased peak o2 consumption for escherichia coli and staphylococcus aureus to 157% (p less than 0.05) and 199% (p less than 0.02), respectively, of their o2 consumption when suspended in hanks balanced salt solution, compared with a 356% increase for neisseria gonorrhoeae with serum. dialyzed serum lacked stimulatory capacity. bac ...	1986	3086230
polyclonal antibody formation of human lymphocytes to bacterial components.	the capacity of various bacterial components to induce antibody formation in human lymphocyte cultures was studied in the present investigation. antibody levels were determined by an enzyme-linked immunosorbent assay (elisa). lipopolysaccharide (lps), bacterial cell walls (cw, isolated from bacillus subtilis and staphylococcus aureus wood 46) and peptidoglycans (pg) appeared to stimulate igm, igg and iga secretion, whereas lysozyme-solubilized pg and teichoic acids (ta) were ineffective. also, u ...	1986	3089918
the primary structure of apolipoprotein a-i from rabbit high-density lipoprotein.	the amino acid sequence of rabbit apolipoprotein a-i (apo a-i) has been determined by degradation and alignment of two overlapping sets of peptides obtained from tryptic and staphylococcal digestions. all of the peptides of rabbit apo a-i resulting from digestion by staphylococcal protease were isolated and sequenced except residues 33-37. a digestion with trypsin was employed to find overlapping and missing peptides. the n-terminus of rabbit apo a-i was confirmed by sequencing the intact protei ...	1986	3095115
the influence of interferon-gamma and various phagocytic stimuli on the expression of mhc-class ii antigens on human monocytes--relation to the generation of reactive oxygen intermediates.	human monocytes show a dose-dependent decrease of the mhc-class ii antigen expression (hla-dr and hla-dq) after addition of zymosan or staphylococcus aureus cowan i particles. interferon-gamma did not prevent this process. the expression of mhc-class i antigens was not affected. the dose-response and kinetic curves showed individual differences. an association between the capacity to form reactive oxygen intermediates and the downregulation of mhc-class ii antigen expression was observed. in add ...	1986	3095231
determination of the complete amino acid sequence of recombinant human gamma-interferon produced in escherichia coli.	the complete amino acid sequence of recombinant human gamma-interferon (huifn-gamma) produced in escherichia coli was determined using a gas-phase protein sequencer. the sequence was established by automated edman degradation on the intact protein and its peptides obtained after staphylococcus aureus v8 protease or trypsin digestion. the result was identical to the amino acid sequence predicted from the nucleotide sequence of the cloned huifn-gamma cdna except that it was missing the four carbox ...	1986	3095440
human interferon-gamma acts as a b cell growth factor in the anti-igm antibody co-stimulatory assay but has no direct b cell differentiation activity.	in this study it is illustrated that recombinant human interferon-gamma (ifn-gamma) acts as a b cell growth factor (bcgf) in the anti-igm antibody co-stimulatory assay. a monoclonal antibody that specifically inhibits the biological activities of ifn-gamma blocks its bcgf activity supporting the specificity of the ifn-gamma effect. various ifn-gamma obtained from different sources displayed the same bcgf activity. nonactivated b lymphocytes do not proliferate in response to ifn-gamma. ifn-gamma ...	1986	3097134
effects of interferons-alpha, -beta, and -gamma on human interleukin-2 production.	interleukin-2 (il-2) production was stimulated with staphylococcus enterotoxin a, heat-killed staphylococcus aureus, or concanavalin a (cona) in cultures of human peripheral blood mononuclear cells (pbmc). il-2 was characterized by its effect on il-2-dependent cytotoxic t lymphocytes (ctll cells) and mouse thymocytes. pretreatment of pbmc with greater than or equal to 100 iu/ml of human-alpha (huifn-alpha) or interferon-beta (huifn-beta), or with greater than or equal to 10 iu/ml huifn-gamma for ...	1986	3100666
requirement of macrophages (monocytes) for the induction of interleukin 2 receptors on b lymphocytes.	the role of macrophages (monocytes) for the induction of interleukin 2 receptors (il 2r) on human b lymphocytes was studied by a direct immunofluorescence method with the use of a fluoresceinated anti-il 2r monoclonal antibody and a flow cytofluorometer. highly purified b lymphocytes alone did not induce il 2r on their surface by stimulation with staphylococcus aureus cowan i, anti-mu antibody, or pokeweed mitogen. however, the addition of monocytes successfully induced il 2r on b lymphocytes st ...	1986	3082985
implications for the role of cognate interactions in in vitro human b cell activation by staphylococcus aureus cowan i and pokeweed mitogen.	human b cell-triggering mechanisms were investigated using the polyclonal activators staphylococcus aureus cowan i (sac) and pokeweed mitogen (pwm). when the cultures of b cells, t cells, and monocytes were stimulated for 5 d by sac or pwm, b cells could be activated by both mitogens to proliferate and secrete ig. even when t cells were substituted by t cell-derived soluble factors, sac-stimulated b cells could differentiate into ig-secreting cells. in contrast, interactions of b and t cells for ...	1986	3484752
[animal experiment studies on the modification of the immune system by ofloxacin].	ofloxacin, one of the new group of quinolone antibiotics, was investigated with respect to its possible interactions with different parameters of the immune system. the study was performed in vivo on balb/c-mice treated for seven days with different doses of the antibiotic and in vitro on human phagocytes from peripheral blood. it was found that ofloxacin does not affect cellular or humoral immune responses in mice. moreover, human phagocytic cells exposed in vitro even to high concentrations of ...	1986	3456983
human peripheral blood b lymphocyte subpopulations: functional and phenotypic analysis of surface igd positive and negative subsets.	highly purified human peripheral blood b cells stimulated with cowan i staphylococcus aureus (sa) and mitogen-activated t cell supernatants (t supt) generated large numbers of immunoglobulin (ig)-secreting cells (isc), whereas fewer isc developed in cultures containing t supt in the absence of sa. to determine whether surface ig isotype expression defined responsive b cell subsets, igd+ and igd- b cells were prepared with the fluorescence-activated cell sorter. whereas both the igd+ and igd- b c ...	1986	3484392
sequential chromosome abnormalities in b cell chronic lymphocytic leukemia: a study of 13 cases.	the chromosomal constitution of stimulated lymphocytes in 13 patients with b cell chronic lymphocytic leukemia (b-cll) were sequentially examined using polyclonal b cell activators (pba), i.e., epstein-barr virus (ebv), lipopolysaccharide w from e. coli (lps), pokeweed mitogen (pwm), and protein a from staphylococcus aureus (pa). of the 11 patients (44 samplings) with abnormal clones, 2 patients had only trisomy 12, 6 patients had trisomy 12 plus other clonal abnormalities, such as +8, +9, +16, ...	1986	3484671
staphylococcus aureus cowan i. potent stimulus of immunoglobulin m rheumatoid factor production.	these studies demonstrate that staphylococcus aureus cowan i (sac), a protein a-positive staphylococcal strain, is a potent and consistent inducer of igm rheumatoid factor production by normal human peripheral blood mononuclear cells. the frequency and magnitude of this response greatly exceeded that of parallel cultures stimulated with pokeweed mitogen or the protein a-negative s. aureus wood strain, although all three agents induced a similar amount of total igm. cell fractionation studies ind ...	1986	3489006
morphological studies on plasma cell differentiation induced by staphylococcus aureus cowan i strain in human peripheral blood b-lymphocytes.		1986	3486541
activation of human b cells: involvement of surface immunoglobulin as evidenced by two biochemically distinct types of response to staphylococcus aureus.	if activation of human b cells by staphylococcus aureus proceeds through interaction of surface immunoglobulin with staphylococcal protein a, then immunoglobulins should be produced that are capable of binding to protein a as a consequence of such stimulation. in the present report it is shown that two biochemically distinct types of response to s. aureus are demonstrable in human peripheral blood lymphocytes. two types of igm are produced: igm capable of binding to protein a, and igm that does ...	1986	3486859
the in vitro effects of methotrexate on the phagocytosis and intracellular killing of staphylococcus aureus by human neutrophils.	the in vitro effect of therapeutic concentrations of methotrexate on the phagocytosis and intracellular killing of staphylococcus aureus by circulating human neutrophils was assessed. neutrophils were isolated from whole blood of six healthy human volunteers by density centrifugation and incubated with 10(-3) m methotrexate. staphylococcus aureus was opsonized in human serum and added to the prepared neutrophils. phagocytosis was determined by serial dilutions and plating of unphagocytized bacte ...	1986	3697933
abilities of human oligodendroglial cells and mouse schwann cells to phagocytose mycobacterium leprae and other mycobacteria.	human oligodendroglial kg-1-c cells derived from human cerebral mixed glioma and mouse schwann cells derived from dorsal root ganglion were studied with respect to their abilities to phagocytose various mycobacteria, especially mycobacterium leprae, and other microorganisms. kg-1-c cells phagocytosed m. leprae at a markedly higher rate than balb/3t3, bhk 21, hela s3, mks-a tu-7, xc, tsv-5, n-18, and schwann cells but at a lower rate than peritoneal macrophages. schwann cells also exhibited subst ...	1986	3510165
induction of c-myc expression in human b lymphocytes by b-cell growth factor and anti-immunoglobulin.	purified human b lymphocytes were examined for transcriptional expression of c-myc in response to mitogenic stimulation by the method of in situ hybridization using 35s-labeled dna probes. the level of c-myc expression increased 10- to 20-fold within 2 hr after the addition of anti-mu, formalinized staphylococcus aureus cowan strain i, or b-cell growth factor, as compared to resting b cells. after 72-96 hr of mitogenic stimulation, c-myc expression remained elevated 5-fold, but expression among ...	1986	3513176
[microbiologic findings in nephrology].	infections of the urinary tract belong to the most frequently encountered bacterial diseases of man. up to 20% of urinary tract infections take a chronic course and thus give rise for complications. culture and identification of microorganisms as well as susceptibility testing are an essential part of the diagnostic procedures and give a basis for specific treatment. bacteriological reports have an increasing importance also for the physician in private practice, since therapy failures and compl ...	1986	3515774
bactericidal action of eosinophils from normal human blood.	the ability of normal human eosinophils to ingest and kill staphylococcus aureus and escherichia coli was investigated and compared with the reactions shown by neutrophils from the same donors. the rate of phagocytosis of s. aureus by eosinophils was 50% of that shown by neutrophils. unlike neutrophils, eosinophils were not able to kill ingested s. aureus at low bacterium/phagocyte ratios. the degree of s. aureus killing increased with increasing ratios, being equal to that of neutrophils when b ...	1986	3522428
microbial filtrates activate granulocytes without complement or prostaglandins.	cardiorespiratory dysfunction in sepsis may be mediated by circulating complement, activated leukocytes, prostaglandins, or by a direct effect of endotoxin. the purposes of this study were to determine if pathogenic microbes produce these substances and to evaluate the direct effects of substances released by micro-organisms on granulocyte aggregation (ga). escherichia coli, (e. coli), aeromonas hydrophila (aeromonas h.), staphylococcus aureus (s. aureus), and candida albicans, (candida a.) were ...	1986	3524892
effect of ciprofloxacin on intracellular organisms: in-vitro and in-vivo studies.	ciprofloxacin is taken up rapidly by both human neutrophils and mouse peritoneal macrophages. it does not appear to be bound firmly within the cell and can be eluted readily if the extracellular concentration is lowered. uptake does not depend on an active transport mechanism. intracellular ciprofloxacin is biologically active reducing the survival of intracellular staphylococcus aureus and mycobacterium fortuitum, in vitro. in vivo, ciprofloxacin was effective in treating a murine systemic infe ...	1986	3542948
the pulmonary disposition of theophylline and its influence on human alveolar macrophage bactericidal function.	we studied the pulmonary disposition of theophylline by performing bronchoalveolar lavage on 19 normal, nonsmoking volunteers who had taken theophylline orally for 14 days. in addition, we determined the influence of theophylline on human alveolar macrophage bacterial phagocytosis, intracellular killing, and hydrogen peroxide release. we found a 1:1 relationship between serum and bronchoalveolar lavage theophylline concentrations when lavage fluid concentrations were corrected for saline dilutio ...	1986	3789521
the amino-acid sequence of rat cu-zn superoxide dismutase.	the primary structure of cu-zn superoxide dismutase isolated from rat liver was determined. the enzyme was reduced, carboxymethylated and fragmented by treatment with cyanogen bromide, trypsin or staphylococcus aureus proteinase v8. the resulting peptides were separated by gel filtration or high performance liquid chromatography and sequenced by automated edman degradation. the total sequence of 153 amino-acid residues per subunit was reconstructed from overlapping peptides. rat cu-zn superoxide ...	1986	3790250
effect of human serum on inhibition of growth of staphylococcus aureus by antimicrobial agents.		1986	3743559
use of a synthetic peptidoglycan-precursor immunogen and its antibodies as a probe of infectious diseases in man.	antibodies to a peptide sequence found in peptidoglycan (pg)- precursors (lys-d-ala-d-ala) have been found in man and their titers are elevated in several human diseases. antibodies in rabbits were elicited by a synthetic immunogen containing these pg-precursor sequences, affinity purified with the precursor linked to sepharose and shown to cross-react with bacterial by-products, such as the high molecular weight soluble peptidoglycan (spg) from staphylococcus aureus, which contain these sequenc ...	1986	3743902
isolation and amino acid sequence of bovine platelet factor 4.	bovine platelet factor 4 was isolated by affinity chromatography using dextran sulfate sepharose and purified by subsequent gel filtration. the complete amino acid sequence of this 88-residue, 9505-da protein was determined by isolation and analysis of the overlapping peptides from tryptic and staphylococcus aureus hydrolysates of reduced, carboxymethylated, and reductive methylated protein. primary structure comparison was made between bovine platelet factor 4, human platelet factor 4, and huma ...	1986	3767377
non-tropical pyomyositis.	pyomyositis occurred in a man who had not been to the tropics. the condition is common in the tropics but most unusual in temperate climates and is nearly always caused by staphylococcus aureus. pyomyositis must be borne in mind in obscure cases of sepsis, as early recognition and treatment are essential to prevent a fatal outcome.	1986	3782487
the effect of 1,25-dihydroxyvitamin d3 on in vitro immunoglobulin production in human b cells.	1,25-dihydroxyvitamin d3 (1,25(oh)2d3) dose-dependently suppressed immunoglobulin (ig) production of human b cells, as evaluated by igg-plaque-forming cells (igg-pfc) in the culture of pokeweed mitogen (pwm)-activated b cells. similar suppressive effect of 1,25(oh)2d3 on ig production of b cells was observed in the staphylococcus aureus cowan i(sac)-induced ig-producing system. the mean percentage of inhibitions at a concentration of 10(-9) m were 60.0 +/- 8.2% (mean +/- se, n = 6) and 65.1 +/- ...	1986	3519769
binding of staphylococcus aureus by human serum spreading factor in an in vitro assay.	human serum spreading factor, an animal cell adhesion-promoting glycoprotein, bound staphylococcus aureus in an assay in which association of bacteria to protein-precoated microtiter wells was quantitated. interaction of human serum spreading factor with s. aureus suggests that this protein may play a role in host defenses and in the invasiveness and pathogenicity of microbial infections.	1986	3710583
modulation of igg effector functions by a monovalent fragment of staphylococcal protein a.	the monovalent v-1 fragment of protein a (fspa) with a mol. wt of 13,000 obtained from an u.v. mutant of staphylococcus aureus cowan i strain was proved to be able to modulate significantly some of the effector functions of igg, such as complement fixation, catabolism, attachment to fc receptors and antibody-dependent cell-mediated cytotoxicity. moreover fspa-like protein a obtained from the a676 strain is mitogenic and enhances nk activity of human peripheral lymphocytes. the efficiency of fspa ...	1986	3724757
rapid method for the detection of group b streptococci from human sources.	a modification of the classical camp test has been devised for the rapid detection of streptococci of serological group b from human sources. the method was compared with detection based on the development of orange pigmented colonies on a starch-based medium and with detection by conventional methods. in a survey of vaginal carriage of group b streptococci in parturient women, the modified camp test detected a carriage rate of 13.09%, the starch-based, pigment enhancing medium, 5.76% and the co ...	1986	3536831
an in vitro investigation of the intracellular bioactivity of amoxicillin, clindamycin, and erythromycin for staphylococcus aureus.	the intraphagocytic bioactivities for staphylococcus aureus of amoxicillin, clindamycin, and erythromycin (0.0075-20 micrograms/ml) were measured in human polymorphonuclear leukocytes (pmnls) with the combination of a fluorochrome microassay and a radioassay. pmnls with normal or depleted membrane-associated oxidative metabolism were used to investigate the interactions that may occur between the intrinsic o2-dependent antimicrobial systems of human phagocytes and antimicrobial agents in the eli ...	1986	3950443
monoclonal antibody recognizing pre-s(2) epitope of hepatitis b virus: characterization of pre-s(2) epitope and anti-pre-s(2) antibody.	a hybrid cell line producing monoclonal antibodies recognizing an epitope encoded by the pre-(s)2 region of hepatitis b virus (hbv) genome was obtained by fusion of mouse myeloma cells with lymphocytes from mice immunized with hbv. the monoclonal antibody mo-f124 secreted from the hybrid line reacted with the pre-s(2) epitope expressed on the surface of both viral and recombinant hbsag particles--pre-s(2) and s gene product--localised on 34 kd glycoprotein of the viral envelope. the pre-s(2) epi ...	1986	2430050
[range of the antigenic specificity of peptidoglycan in staphylococcus aureus compared to other representatives of the staphylococcus genus].	in investigations based on the use of a highly sensitive test system permitting the detection of normal human antibodies to s. aureus peptidoglycan, the antigenic relationships between the peptidoglycans of s. aureus and other representatives of the genus staphylococcus have been studied. among other staphylococcal species, s. simulans, s. xylosus, s. hyicus, s. cohnii, s. hyicus s. s. chromogenes have been found to possess peptidoglycans most closely related to s. aureus peptidoglycans, while s ...	1986	2428191