Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| bacteriolytic activity of human gingival exudate. | we investigated the bacteriolytic activity of gingival crevicular fluid (cf) on 14c-labeled streptococcus faecalis, streptococcus mutans, staphylococcus aureus, and on whole dental plaque. cf was collected from 100 healthy donors pooled and centrifuged at 200 g. cf supernate and a frozen and thawed extract of the pellet were interacted with the different bacterial strains, while streptococcus faecalis and staphylococcus aureus released 60% and 75% of the radioactive label, only 38% of it was sol ... | 1980 | 6771214 |
| effects of human neutrophils and monocytes on nocardia asteroides: failure of killing despite occurrence of the oxidative metabolic burst. | to study mechanisms of resistance to nocardia asteroides in vitro, suspensions of human neutrophils and monocytes were challenged with n. asteroides in the presence of autologous serum. at 4 hr, numbers of viable n. asteroides incubated with neutrophils and monocytes averaged only 10% and 21% less, respectively, than numbers of n. asteroides incubated without phagocytes. in contrast, a mean of > 90% of listeria monocytogenes and staphylococcus aureus incubated with neutrophils and monocytes was ... | 1980 | 6777435 |
| use of immunoglobulin-loaded protein a-bearing staphylococci as a primary solid phase immunoadsorbent in radioimmunoassay. | protein a-bearing formalinized heat-inactivated staphylococcus aureus bind rabbit 125i-igg with high capacity of igg for antigen. the affinity of immobilized igg for antigen is equal to the affinity of soluble antibody, and the capacity for antigen approximates the capacity of soluble antibody for antigen. 125i-igg bound to high affinity sites on bacteria is not substantially displaced in the presence of human serum after 4 h at 4 degrees c, but rabbit immunoglobulin can displace bound rabbit ig ... | 1980 | 6965378 |
| effect of cyclosporin a on t-dependent and t-independent immunoglobulin synthesis in vitro. | an immunosuppressive cyclic polypeptide, cyclosporin a (cya), isolated originally from two species of fungi, prolongs organ allograft survival in several species. however, virtually nothing is known about the pharmacokinetics of this substance and its mode of action in man. we have quantified the impact of cya on t-dependent and t-independent immunoglobulin (ig) synthesis of human blood leukocytes in vitro, and on the proliferating capacity of the interacting lymphoid cell populations--t cells, ... | 1980 | 6968408 |
| human lymphocyte colony formation: a comparison of stimulation with intact staphylococcus, staph, protein a, pokeweed mitogen and phytohemagglutinin. | the efficacy of various mitogens for growing human lymphocyte colonies in semisolid agar cultures was tested. protein a as well as phytohemagglutinin (pha) exhibited a strong stimulatory capacity in contrast to cowan i strain of staphylococcus aureus (cowan i). pokeweed mitogen (pwm) was effective and generated a sizeable number of colonies. plating efficiency of lymphocytes from different donors and for the same donors cultured on different days were highly variable. regardless of mitogen, colo ... | 1980 | 6969477 |
| low responsiveness of human neonatal lymphocytes to a b-cell mitogen, staphylococcus aureus cowan i (spa coi). | responses of neonatal and adult lymphocytes to various mitogens were studied. lymphocytes from umbilical cord blood (ucb) responded well to both phytohemagglutinin and concanavalin a, and also to pokeweed mitogen and staphylococcus aureus protein a. the responses of ucb lymphocytes to these mitogens were not significantly lower than those of adult peripheral blood lymphocytes (pbl). in contrast, ucb lymphocytes showed only a minimal response to killed staphylococcus aureus cowan i (spa coi), a p ... | 1980 | 6971389 |
| peptide mapping by limited proteolysis in sodium dodecyl sulfate of the main intrinsic polypeptides isolated from human and bovine lens plasma membranes. | 1980 | 6988013 | |
| kinetics of intracellular killing of staphylococcus aureus and escherichia coli by human granulocytes. | 1980 | 7000519 | |
| bactericidal properties of lysosomal proteins obtained from human polymorphonuclear leucocytes (pmnl) examined in vitro. ii. morphological estimation of cells treated with lysosomal proteins. | the present paper is concerned with the action of lysosomal proteins isolated from granulocytes of human peripheral blood on gram-plus and gram-minus bacteria. differences in bacterial sensitivity to these proteins were found. the highest bactericidal activity was observed while bacteria from staphylococcus and escherichia were treated with lysosomal proteins. no structural changes in cells of bacteria from streptococcus and mycobacterium tuberculosis were observed. the sensitive bacteria cell w ... | 1980 | 7001810 |
| amino acid sequence and location of the three glycopeptides in the fc region of human immunoglobulin d. | 1980 | 7008791 | |
| measurements of extracellular immunoglobulins as an index of b cell function. | we have developed a method for measuring the activation or suppression of b cells in culture by determination of immunoglobulins released into the extracellular fluid, using a conventional, nonisotopic assay. to avoid the interference of human serum proteins in the assays, cultures were established in medium supplemented with fetal calf serum. this caused some b cell activation, as reflected by the release of small amounts of immunoglobulins (1.5 to 0.7 microgram/ml igg, 0.8 to 1.4 microgram/ml ... | 1980 | 7019022 |
| circulation of enterotoxigenic strains of staphylococcus aureus in humans and their environment. | in the study of eight groups of staphylococci covering 870 strains of staphylococcus aureus, isolated from humans and ten kinds of animals, it was found that a total of 271 strains [31.4%] produced some of the types of staphylococcal enterotoxin [se]. with the exception of 34 strains isolated from staphylococcal enterotoxicosis the other strains were unrelated to this disease. while among healthy persons 14% [students]---57% [employees of food industry undertakings] enterotoxigenic strains were ... | 1980 | 7190989 |
| plasma membrane-associated antigens on tumor cells derived from transitional-cell carcinoma of the human urinary bladder. ii. identification at the molecular level of plasma membrane-associated antigens. | the surface proteins of seven human cell lines (three bladder carcinomas (tcc), two normal urothelial lines, one colon carcinoma, and one malignant melanoma) were labelled with 125i by the glucose oxidase-lactoperoxidase technique. plasma membranes of the cells were isolated and analysed by sodium dodecyl sulphate electrophoresis (sds-page). when analysed under reducing conditions by staining with protein stain, approximately 45 distinct membrane polypeptides were detected in all membrane prepar ... | 1980 | 7203713 |
| mammalian bite wounds. | clinical data were collected prospectively from a series of 160 patients presenting with mammalian bite wounds. anaerobic and aerobic cultures were prepared from sterile swabs placed in 65 bite wounds prior to cleansing. infection was noted in 11 of 22 cat bites, six of 37 human bites, three of 80 dog bites, and in none of the 21 bites caused by other mammals. pasteurella multocida was recovered from six infected cat and dog bites, all of which developed infection within 24 hours of injury. stap ... | 1980 | 7356213 |
| solid-phase radioimmunoassay with protein-a-bearing staphylococcus aureus cells used to assay a protein (ferritin) and a hapten (digoxin). | we compared use of protein-a-containing staphylococcus aureus bacteria with conventional ammonium sulfate precipitation and second-antibody methods of separating bound and free antigen in the radioimmunoassay of a hapten (digoxin) and protein (ferritin) in human sera. in each case, values obtained with the heat-killed, formalin-fixed bacteria correlated well with those found by established methods. no matrix effects were detected in either hapten or protein measurements. because of the affinity ... | 1980 | 7356569 |
| a new haemolysin from staphylococcus aureus which lyses horse erythrocytes. | a new haemolysin from staphylococcus aureus produced opaque zones of haemolysis on horse blood agar but did not lyse equine erythrocytes suspended in phosphate-buffered saline. the haemolysin was not neutralized by normal rabbit serum and was distinct from alpha-, beta- and delta-haemolysins as well as human leucocidin. partially purified preparations produced erythema when injected intradermally into rabbit skin. | 1980 | 7365453 |
| identity and interspecific transfer of gentamicin-resistance plasmids in staphylococcus aureus and staphylococcus epidermidis. | the hypothesis that emergence of gentamicin-resistant strains of staphylococcus aureus and staphylococcus epidermidis in a neonatal special care nursery was the result of transfer of a single plasmid between these two species was examined. in experiments with mixtures of staphylococci, either in mixed cultures or on human skin, isolates of s. aureus and s. epidermidis transferred their gentamicin-resistance plasmids both intra- and interspecifically. by electron microscopy, the molecular masses ... | 1980 | 7391616 |
| clinical and pharmacokinetic evaluation of parental cefoxitin in infants and children. | thirty-two infants and children ranging in age from 3 to 151 months (mean, 26 months) were treated with parenteral cefoxitin (150 mg/kg per day). ten patients with isolates of haemophilus influenzae (six with cellulitis, two with arthritis, and two with mastoiditis), four with staphylococcus aureus (one with lymphadenitis, one with septicemia, and two with abscess), and three patients with streptococcus pneumoniae (one each with cellulitis, abscess, and arthritis), were clinically and bacteriolo ... | 1980 | 7396456 |
| [hemoglobins, xxxii. analysis of the primary structure of the monomeric hemoglobin ctt viia (erythrocruorin) or chironomus thummi thummi, diptera (author's transl)]. | the dimeric hemoglobin ctt viia (erythrocruorin) was isolated from the hemolymph of the larva from chironomus thummi thummi and purified by preparative polyacrylamide gel electrophoresis. peptides obtained by limited tryptical digestion were sequenced by automatic edman degradation. for the elucidation of the sequence in the c-terminal region of the chain, additional cleavages with proteinase of staphylococcus aureus and chymotrypsin were necessary. ctt viia is compared with human beta-chains an ... | 1980 | 7399413 |
| [detection of "staphylococcus aureau" protein a on selective culture media (author's transl)]. | production of protein a by 100 various animal strains and 106 human strains of staphylococcus aureus has been detected by conditioned haemagglutination after growth on seven different culture media: agar medium at n degrees 2 and columbia agar with and without nalidixic acid (10.8 micrograms/ml), baird-parker, chapman and tellurite-glycine-agar media. rate of protein a-producing strains is as high on nalidixic acid media as on the same media without antibiotic. baird-parker, chapman and tellurit ... | 1980 | 7425451 |
| experimental osteomyelitis due to staphylococcus aureus or pseudomonas aeruginosa: a radiographic-pathological correlative analysis. | a previously-described experimental model of bacterial osteomyelitis was used to investigate systematically the sequential radiographic and histopathological changes in the tibias of rabbits infected with either staphylococcus aureus or pseudomonas aeruginosa. the radiographic changes induced by both organisms were progressive, increasing in severity from the first to the fourth week after infection. the severity and extent of radiographic changes, especially that of bond destruction, were signi ... | 1980 | 7426395 |
| modulation of human polymorphonuclear leukocyte chemotaxis by leukocyte extracts, bacterial products, inflammatory exudates, and polyelectrolytes. | human polymorphonuclear leukocytes (pmn) chemotaxis was tested during exposure to leukocyte and platelet extracts, a variety of polyelectrolytes, inflammatory exudates, and bacterial products. the chemoattractants employed were either zymosan-activated serum or supernatant from autolyzed staphylococcus aureus. chemotaxis to both chemoattractants was markedly inhibited by leukocyte and platelet extracts; inflammatory exudates; anionic polyelectrolytes, dna, hyaluronic acid, liquoid; and by cation ... | 1980 | 7429609 |
| mitogenic responsiveness of mouse, rat and human lymphocytes to staphylococcus aureus cell wall, teichoic acid, and peptidoglycan. | the mitogenic activity of staphylococcus aureus peptidoglycan (pg), teichoic acid (ta) and cell wall (cw, a pg-ta complex) for mouse, rat and human lymphocytes was determined by measuring dna synthesis. at optimal concentrations pg was the most potent mitogen, inducing maximum stimulation of mouse spleen lymphocytes on day 2 of culture, rat spleen lymphocytes on day 3, rat lymph node cells on day 2, human peripheral blood lymphocytes on day 5, and human cord blood lymphocytes on day 4. cw was le ... | 1980 | 7429652 |
| platelet interaction with bacteria. vi. contrasting the role of fibrinogen and fibronectin. | the roles of fibrinogen and fibronectin were contasted in the responses of human platelets to staphylococcus aureus and collagen. congenital afibrinogenemic (ca) platelets and washed normal platelets had delayed aggregation due to a prolonged latent phase in response to contact with the bacteria when fibrinogen was absent from the suspending media. when a physiologic level of human fibrinogen was added to the ca platelets in autologous plasma or to the washed platelets in balanced saline, the ti ... | 1980 | 7435500 |
| effect of extracellular serum in the stimulation of intracellular killing of streptococci by human monocytes. | this study shows that the intracellular killing of streptococcus pyogenes, streptococcus faecalis, and streptococcus pneumoniae by human monocytes is stimulated by the extracellular presence of both heat-stable and heat-labile serum factors. a similar kind of stimulation of monocytes has been described in respect of catalase-positive microorganisms. however, killing of these bacteria is negligible in the absence of extracellular serum factors, whereas a large proportion of the ingested catalase- ... | 1980 | 7439986 |
| bactericidal activity of cerumen. | freshly collected cerumen (dry form) suspended at a concentration of 3% in glycerol-sodium bicarbonate buffer showed bactericidal activity against some strains of bacteria tested. this suspension reduced the viability of haemophilus influenzae, escherichia coli k-12, and serratia marcescens by more than 99%, whereas the viability of two pseudomonas aeruginosa isolates, e. coli k-1, streptococcus, and two staphylococcus aureus isolates of human origin was reduced by 30 to 80%. the results support ... | 1980 | 7447422 |
| binding of murine myeloma proteins of different ig classes and subclasses to fc-reactive surface structures in gram-positive cocci. | twelve different murine myeloma proteins were tested for binding to seventy gram-positive strains belonging to group a, c and g streptococci and to staphylococcus aureus. group a streptococci, known to bind human igg, were incapable of binding any of eight murine igg immunoglobulins tested except for one strain that bound an igg2b myeloma protein. in contrast, group c and g streptococci interacted with murine immunoglobulins of subclasses igg2a, igg2b and igg3, and g strains also to a lesser ext ... | 1980 | 9537027 |
| expression of hla-abc and -dr locus antigens on human kidney, endothelial, tubular and glomerular cells. | the distribution of the major histocompatibility complex antigens on the various cellular structures of the human kidney was analysed by using a modified staphylococcus aureus cowan 1 method. conventional alloantisera and heterologous antisera raised against isolated molecules were used for the hla-abc antigens, whereas the hla-dr (ia) antigens were detected with heterologous antisera only. the hla-abc antigens were expressed on all types of kidney passenger leucocytes, on vascular endothelial c ... | 1980 | 9537058 |
| sensitivity of staphylococci to fatty acids: novel inactivation of linolenic acid by serum. | the inhibition of coagulase-negative staphylococci and staphylococcus aureus of human or animal origin by most free fatty acids was similar, but coagulase-positive staphylococci were sensitive and coagulase-negative cultures were resistant to linolenic acid. animal strains of s. aureus were more sensitive to linolenic acid than were human strains. these differences were reflected in the relative abilities of the three categories of strains to survive on human skin. the antibacterial effects of 2 ... | 1981 | 7463466 |
| binding of radiolabeled staphylococcus aureus delta-toxin to human erythrocytes. | the addition f [3h]isoleucine to a chemically defined medium resulted in the production of delta-toxin (dt) with a high specific radioactivity (0.47 microci/mg). the purified tritium-labeled toxin ([3h]dt) was found to migrate in sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a single band with a molecular weight of 1,600. upon electrofocusing, [3h]dt yielded one major peak (pi = 5.90) and two minor peaks (pi = 5.10, 6.95) of radioactivity. the percentage of [3h]dt associated with ... | 1981 | 7228396 |
| chemical and carbon-13 nuclear magnetic resonance studies of the blood group m and n active sialoglycopeptides from human glycophorin a. | the nh2-terminal sialoglycopeptides from human erythrocyte glycophorin a have been obtained by specific proteolytic cleavage and gel filtration chromatography. by cyanogen bromide cleavage, a glycosylated octapeptide was obtained from blood group m donors having an amino acid composition and 13c nmr spectrum consistent with the structure (formula: see text) was demonstrated. by staphylococcus aureus protease cleavage, a glycosylated pentapeptide was obtained from n donors having the same structu ... | 1981 | 7240172 |
| abnormalities in membrane phospholipid organization in sickled erythrocytes. | in contrast to the wealth of information concerning membrane phospholipid asymmetry in normal human erythrocytes, very little is known about membrane phospholipid organization in pathologic erythrocytes. since the spectrin-actin lattice, which has been suggested to play an important role in stabilizing membrane phospholipid asymmetry, is abnormal in sickled erythrocytes, we determined the effects of sickling on membrane phospholipid organization. we used two enzymatic probes: been venom phosphol ... | 1981 | 7240412 |
| [toxic-shock syndrome. three cases (author's transl)]. | several cases of toxic shock syndrome (t.s.s) have been recently reported from the u.s.a. clinical features of this new syndrome include fever, desquamative scarlatiniform rash, hypotension and involvement of central nervous system, liver, kidney and muscles. more than 90% of cases are women with staphylococcic vaginitis using tampons during menstruations. a toxin produced by staphylococcus aureus is thought to be the causative agent, because the germ has been isolated in local (vaginal, pharyng ... | 1981 | 7267317 |
| [addition to the scheme of ecologic variants of staphylococcus aureus]. | altogether 55 s. aureus strains were isolated from the nose and skin of 2 monkey species (cercopithecus sabaeus and macaca fascicularis). the strains were studied in 19 biological tests. 45 of these strains were found to differ in their biological properties from the ecological s. aureus variants described in literature. of these grounds the strains were classified with the new ecovar simiae comprising 2 subvariants. the strains of the ecovar simiae coagulated human plasma, did not coagulate bov ... | 1981 | 7282191 |
| [resistance development in human staphylococcus aureus due to the use of oxytetracycline and tylosin in industrialized swine production units]. | staphylococcus aureus strains from human nasal carriers without vocational contact with pigs could be differentiated from the majority of strains obtained from pathologically affected pigs by determination of host-specific-variety, according to meyer (1966) and witte et al. (1977). less unambiguous findings regarding site variability were recorded from strains in nasal mucosa of pig-handling personnel, clinically intact pigs on farms, and slaughtered pigs. antibiotics, such as oxytetracycline an ... | 1981 | 7283644 |
| antibody levels to bacterial peptidoglycan in human sera during the time course of endocarditis and bacteremic infections caused by staphylococcus aureus. | sera from patients with endocarditis and bacteremia due to staphylococcus aureus were compared for peptidoglycan-binding capacity with those from normal blood donors. those patients treated with beta-lactam antibiotics had higher antigen-binding levels than normal donors and patients treated exclusively with vancomycin (p less than 0.01). the factor responsible for this activity was purified by affinity chromatography from a normal donor and shown to be an immunoglobulin. specificity studies ind ... | 1981 | 7287182 |
| comparison of mechanisms of interaction between protein a from staphylococcus aureus and human monoclonal igg, iga and igm in relation to the classical fc gamma and the alternative f(ab')2 epsilon protein a interactions. | four purified human monoclonal igg, iga and igm proteins were tested for their inhibitory effect on the binding of protein-a-reactive 125i-ige and 125i-fc gamma, respectively, to protein-a-sepharose. only igg myeloma proteins significantly inhibited the binding of 125i-fc gamma to protein-a-sepharose, whereas most, but not all, myeloma proteins, irrespective of their immunoglobulin class and with varying efficiency, inhibited the binding of protein-a-reactive 125i-ige to protein-a-sepharose. the ... | 1981 | 7302528 |
| variability in the degree of opsonization and phagocytosis of strains of staphylococcus aureus isolated from patients with disseminated intravascular coagulation. | patients with septicemia due to staphylococcus aureus occasionally manifest dic. we studied the ability of organisms isolated from 30 patients with s. aureus (5 with dic) to be opsonized and phagocytosed by normal human pmns in the presence of normal human serum chelated with mgegta as a possible measure of alternative complement pathway activation. by this assay, nine s. aureus strains were found to be opsonized at 50% level or greater. five of those organisms were isolated from the five patien ... | 1981 | 7310231 |
| radioimmunoassay of human adenosine deaminase. | 1981 | 7321887 | |
| classification of macrolide-lincosamide-streptogramin-b-type antibiotic resistance determinants. | we have examined the relationship between the genes specifying resistance towards macrolid-lincosamide-streptogramin-b-type (mls) antibiotics from group d and h streptococci, staphylococcus aureus, bacillus licheniformis, and bacteroides fragilis by dna annealing. we detected homology between the mls resistance determinants from the gram-positive cocci (streptococci and staphylococci) but not between these and the genes from b. licheniformis and b. fragilis. based on these dna-dna hybridizations ... | 1981 | 7332186 |
| enterotoxin production by staphylococcus aureus related to the origin of the strains. | the production of enterotoxins by strains of staphylococcus aureus of human and animal origin seems to be common. 104 out of 170 strains (61%) produced one or more of the a, b, and c enterotoxins. strains from cow and milk often produced enterotoxin c, and enterotoxin a producing strains were mainly isolated from dogs. human food poisoning seemed in our material to be induced by enterotoxin a producing strains. | 1981 | 7336929 |
| the clinical spectrum of toxic shock syndrome. | toxic shock syndrome (tss) is a recently recognized acute multisystem illness that may recur. epidemiologic links with menstruation and use of tampons have been identified. we report the cases of seven patients (six women and one man), 12 to 31 years old, seen over nine months, who met the criteria for tss. four were menstruating at onset. all had hypotension, fever, erythematous rash and distal desquamation. a prodrome of myalgias and diarrhea occurred in all patients. clinical features of the ... | 1981 | 7340115 |
| protein a-like activity and streptococcal cross-reactions. | recognition of the protein a-like activity of some strains of group a streptococci has thrown doubt on much previous work suggesting antigenic cross-reactions between these streptococci and mammalian tissues. the strains used in our previous studies have now been examined by the mixed reverse passive antiglobulin reaction (mrpah) for the 'non-specific' absorption of purified fc portion of human igg. they were found to have only traces of activity. the strain of staphylococcus aureus used to cont ... | 1981 | 7039880 |
| tumor-associated bacteria capable of producing a human choriogonadotropin-like substance. | aerobic microorganisms were isolated and identified from 9 of 10 malignant tissues aseptically obtained from surgical patients. the organisms isolated are species commonly associated with the flora of the human body. when these cancer-associated organisms were grown in trypticase soy broth (bbl microbiology systems), a protein substance was isolated from the culture filtrates by acetone precipitation. the acetone precipitates of 12 of 14 organisms tested were positive when assayed by radioimmuno ... | 1981 | 7019090 |
| malakoplakia: evidence for an acquired disease secondary to immunosuppression. | a 44-year-old man was maintained on prednisone and azathioprine after renal transplantation. one and a half years after transplantation, he developed multiple perianal escherichia coli abscesses. these became chronic and resisted antibiotic therapy. histologically, the lesions were typical of malakoplakia. peripheral blood neutrophils and monocytes had impaired killing of staphylococcus aureus and e. coli in vitro. no abnormality of hexose monophosphate pathway activity, tetrazolium dye reductio ... | 1981 | 7020170 |
| application of partition affinity ligand assay (pala) in quick test for quantitation of staphylococcus aureus bacterial cells. | a novel immunochemical method for the quantitation of bacterial cells is described. the method, which is based on separation of bound from free reactants in aqueous two-phase systems has been studied both as direct and competitive binding assays, with a system consisting of intact cells of staphylococcus aureus and human immunoglobulin g molecules. to achieve high resolution, one of the reactants was modified so that the two reactants, the bacterial cells and the immunoglobulin molecules, occurr ... | 1981 | 7021681 |
| detection of igd-secreting human lymphocytes at the single cell level by a protein a plaque assay. | by the use of staphylococcus aureus protein a-coupled erythrocytes as target cells, and anti-ig antibodies as a developing serum, immunoglobulin-secreting cells can be detected as plaque-forming cells in a complement-dependent haemolytic plaque assay. this method is based on the fact that the fc portion of igg will bind to protein a molecules. using a rabbit anti-human igd immunoglobulin as a developing serum we were able to detect secretion of igd molecules from human cells. in a patient with a ... | 1981 | 7026092 |
| analysis of serum igg against treponema pallidum protein antigens in experimentally infected rabbits. | sensitive and highly specific radioimmunoprecipitation and gel electrophoreses-fluorography were used to monitor the serum igg response of experimentally infected rabbits against key protein antigens of treponema pallidum. the sera from six rabbits challenged intradermally (id) and six intratesticularly (it) with t pallidum were examined over a period of four months. a specific antibody response to most treponemal surface proteins (including those ligands implicated as mediating host-cell-surfac ... | 1981 | 7028207 |
| effect of human saliva on glucose uptake by streptococcus mutans and other oral microorganisms. | we examined the effects of human whole salivary supernatant and parotid fluid on glucose uptake by streptococcus mutans, streptococcus sanguis, streptococcus mitis, actinomyces viscosus, staphylococcus aureus, and escherichia coli. the following three effects of saliva were observed: (i) inhibition of glucose uptake (s. mutans, s. sanguis), (ii) promotion of a transient, rapid (0 to 30 s) burst of glucose uptake (s. mutans, s. sanguis), and (iii) enhancement of glucose uptake (s. mitis, a. visco ... | 1981 | 7012014 |
| postantibiotic leukocyte enhancement: increased susceptibility of bacteria pretreated with antibiotics to activity of leukocytes. | exposure of escherichia coli and staphylococcus aureus to high levels of certain antibiotics for brief periods of time increases the susceptibility of the organisms to the antimicrobial action of normal human leukocytes. the degree of sensitization varies with the antibiotic used for the pretreatment of the bacteria and is most pronounced in pretreatment of e. coli with chloramphenicol. studies on the rates of phagocytosis and intracellular killing of e. coli indicate that the postantibiotic leu ... | 1981 | 7012999 |
| polyclonal activation of human peripheral blood b lymphocytes by formaldehyde-fixed salmonella paratyphi b. i. immunoglobulin production without dna synthesis. | a "new" polyclonal activator of human peripheral blood b cells, formaldehyde-fixed salmonella paratyphi b, is described. this bacterium does not stimulate cell proliferation as measured by incorporation of tritiated thymidine but does stimulate a subpopulation of b cells to secrete large amounts of igm, igg, and iga in 7-day cell cultures. the immunoglobulins (ig) produced by cells responding to s. paratyphi b are not specific antibodies against the bacterial antigens. in comparison with other b ... | 1981 | 6972434 |
| postantibiotic suppression of bacterial growth. | persistent suppression of bacterial growth following exposure of both gram-positive and gram-negative bacteria to numerous antimicrobial agents was studied. the persistent, or postantibiotic, effect was quantitated by periodic counts of colony-forming units after removal of the drug by washing, dilution, or inactivation with penicillinase. although a postantibiotic effect was observed with all drugs studied, there were marked differences among drugs in their postantibiotic effects on certain org ... | 1981 | 6784225 |
| human seminal plasma inhibition of antibody complement-mediated killing and opsonization of neisseria gonorrhoeae and other gram-negative organisms. | seminal plasma diluted 1:5-1:1,000 gave marked inhibition of serum antibody complement-mediated bactericidal and opsonic effects against neisseria gonorrhoeae and other gram-negative organisms. serum that was bactericidal at a dilution of 1:5,120 was not bactericidal at a dilution of 1:10 when seminal plasma was added. bactericidal action of immune human or rabbit sera, or purified immunoglobulin (ig)g or igm plus complement for six strains of n. gonorrhoeae, serogroups a, b, c, and y of neisser ... | 1981 | 6785314 |
| expression of biological effector functions by immunoglobulin g molecules lacking the hinge region. | several biological effector functions mediated by sites on the fc region of human igg1 have been studied in two variant igg1 kappa monoclonal proteins (dob and lec) which contain deletions corresponding to the entire hinge region of the heavy chains. neither dob nor lec protein in aggregated form was able to activate the classical complement pathway, and this was shown to be due to an inability to bind the first component of complement (c1). by rosette inhibition assays, dob and lec proteins wer ... | 1981 | 6787591 |
| [causes of the antibacterial action of amniotic fluid (author's transl)]. | the activity of e. coli, group b streptococci, strept. faecalis, stap. epidermidis and staph. aureus in human amniotic fluid was studied. in comparison to standard i broth an inhibited growth of test organisms was observed. the bacteriostatic effect of amniotic fluid was most significant with staph. epidermidis. we further investigated the causes for this bacterial growth inhibition with staph. epidermidis. we showed that it is independent from the initial number of bacteria as well as from zinc ... | 1981 | 6790329 |
| human serum precipitins to human dental plaque and oral bacteria. | 1981 | 6791620 | |
| [studies on the relationship between staphylococcus aureus inhabited in human and staphylococcal food poisoning. 2. a long-term observation of staphylococcus aureus isolated from mouth washings of healthy adults (author's transl)]. | 1981 | 6799595 | |
| [studies on relationship between staphylococcus aureus inhabiting human and staphylococcal food poisoning. 3. the outbreaks of food poisoning due to rice ball contaminated with staphylococci inhabiting human (author's transl)]. | 1981 | 6801152 | |
| functions of sendai virus nucleocapsid polypeptides: enzymatic activities in nucleocapsids following cleavage of polypeptide p by staphylococcus aureus protease v8. | 1981 | 6258313 | |
| resistance transfer in mixed cultures of staphylococcus aureus. | transfer of resistance markers was regularly observed in mixed cultures of pairs of constructed strains of staphylococcus aureus. this transfer was shown to be due to general transduction and required a lysogenic donor strain, carrying a transducing phage of serotype b and a transducible plasmid, and an acceptor strain without either strong restriction or an incompatible plasmid. transfer was sometimes bidirectional when both strains carried a transducing phage. unidirectional transfer occurred ... | 1981 | 6450835 |
| anomalous effect of serum on the antimicrobial activity of cefoperazone. | this study investigated the effect of added serum on cefoperazone activity in vitro. due to protein-binding, an 8-fold difference would be expected between minimum inhibitory concentrations in broth and in broth plus 90% serum. using supplemented mueller-hinton broth (smh) and smh plus 90% heat-inactivated human serum there were differences of 3.36 +/- 0.92 for staphylococcus aureus strain atcc1 25923, 1.39 +/- 0.47 for escherichia coli strain atcc 25922 and 1.43 +/- 0.39 for pseudomonas aerugin ... | 1981 | 6456892 |
| studies on possible cross transmission of mercuric chloride resistant staphylococcus aureus between dogs and kennel attendants. | ten s. aureus strains were each introduced into the nostrils of each of 10 dogs. the dogs retained the strains of s. aureus for the period of the experiment (eight weeks). five of them with human strains of s. aureus, and the two control dogs acquired strains of canine origin during the period, but no human strain was detected in the nostrils of those into which canine strains were introduced. one of the dogs (dog 4) died during the period of the experiment and both human and canine s. aureus we ... | 1981 | 6460716 |
| do well-to-do and repeatedly rejecting renal allografts express the transplantation antigens similarly on their surface? | we have performed fine-needle aspiration biopsies to well-to-do and repeatedly rejecting renal allografts. after biopsy the aspirated cells were treated either (a) with monospecific rabbit antisera to hla-abc, -dr or beta 2m, or (b) with ordinary anti-hla-a and b alloantisera. after wash, the cell-bound antibody was chased with staphylococcus aureus cowan i bacteria and the antibody-binding cell type(s) was analyzed from stained cytocentrifuged cell smears. in normal, non-transplanted human kid ... | 1981 | 6755686 |
| in vitro synthesis of human brain proteins including tubulin and actin by purified postmortem polysomes. | polysomes were prepared from human brain tissue 2-6 h postmortem; the polysomes were active in a cell-free protein synthesis system containing rabbit reticulocyte factors. protein synthesis was totally dependent upon added mgcl2, atp, the reticulocyte factor fraction, and the human polysome fraction. human brain proteins synthesized in the presence of l-[35s]methionine were analyzed by one- and two-dimensional polyacrylamide gel electrophoresis. over 250 proteins were synthesized and they extend ... | 1981 | 6894159 |
| [the activity of imidazole derivatives in the presence of human plasma (author's transl)]. | by warburg technique using yeasts and staphylococci, any decrease in antimicrobial activity of the imidazole derivatives could be excluded in the presence of human plasma. econazole nitrate, the classical topical imidazole derivative, and ketoconazole, an oral active antifungal, were tested. the compatibility between imidazole derivatives and human plasma is of practical importance for obtaining optimal therapeutic results in treating human mycoses. | 1981 | 6267837 |
| comparisons of rotavirus polypeptides by limited proteolysis: close similarity of certain polypeptides of different strains. | the polypeptides of sa11 rotavirus produced in virus-infected cells were analyzed by limited proteolysis, using staphylococcus aureus v8 protease. this clearly distinguished between all the known primary gene products of the virus and allowed relationships between other infected-cell proteins, and between infected-cell and virus structural proteins, to be ascertained. a comparison of the proteolysis cleavage patterns between sa11 rotavirus and the human rotavirus wa was also performed which demo ... | 1981 | 6275113 |
| up-regulation of lactogenic receptors -- an immunological artifact? | we examined whether injection of heterologous hormones for more than one week might evoke a humoral immune response which would simulate receptor induction. male rats were injected daily for ten days with human growth hormone (hgh) or ovine prolactin (oprl), and binding of 125i-hgh and 125i-oprl was examined in serum and in membranes from liver and lung. specific binding of 125i-hgh and 125i-oprl increased in the sera of hgh- and oprl-injected animals, respectively. a marked increase in hgh but ... | 1982 | 6284491 |
| moxalactam concentration in human aqueous humor after intravenous administration. | two grams of moxalactam disodium was administered intravenously to 20 patients before cataract extraction. mean aqueous humor concentrations of moxalactam were 0.92, 0.87, 1.98, 2.13, and 1.24 micrograms/ml 30 minutes and 1, 2, 4, and 6 hours, respectively, after treatment. adequate levels against staphylococcus aureus and staphylococcus epidermidis were not achieved. with the exception of pseudomonas species, therapeutic levels three to ten times greater than the median minimum inhibitory conce ... | 1982 | 6213219 |
| induction of human immunoglobulin secretion. ii. t lymphocyte dependency and radiosensitivity of t-cell help for induction of b-cell differentiation by staphylococcus aureus strain cowan i. | 1982 | 6215128 | |
| changes in camp metabolism during phagocytosis of s. aureus by human monocytes. | involvement of cyclic adenosine 3',5'-mono-phosphate (camp) in the phagocytosis of staphylococci by human monocytes was demonstrated by assay of adenylate cyclase and camp phosphodiesterase (pde). monocyte adenylate cyclase and pde activities were assayed on cell homogenates prepared from monocytes isolated by plate adherence. phagocytosis of staphylococci was associated with diminished adenylate cyclase activity, which reached a minimum after 10 min of incubation (p less than 0.05), and an incr ... | 1982 | 6296386 |
| complete amino acid sequence of bovine beta 2-microglobulin. | beta 2-microglobulin has been isolated and crystallized from bovine colostrum and represents the only crystalline form of this protein reported. the complete sequence of bovine beta 2-microglobulin was determined using only one proteolytic enzyme, staphylococcus aureus v8 protease. automated degradation of the intact molecule and two large peptides produced by enzymic digestion provided unequivocal placement of all residues. bovine beta 2-microglobulin, molecular weight 11,630, contains 98 resid ... | 1982 | 6174509 |
| a quantitative enzyme-linked immunosorbent assay for honeybee venom-specific immunoglobulin g. | a quantitative enzyme-linked immunosorbent assay (elisa) for honeybee venom-specific igg is reported. this elisa surmounts the problem of poor reproducibility due to nonparallelism of dilution curves in previously reported elisas. the assay is performed in polyvinyl "u" microtiter plates in which hbv is physically adsorbed to the wells. the antigen is sequentially overlaid with human serum albumin, unknown serum diluted in 10% normal goat serum (ngs), and peroxidase-labeled anti-human igg in ngs ... | 1982 | 6179974 |
| radioactive human lymphoblastoid interferon. one-step purification, regulation of heterogeneous species production and its use for radioimmunoassay. | human lymphoblastoid interferon (alpha type), labeled with [3h]leucine added to virus-induced namalwa cells, was purified quantitatively and in one step from the culture fluid by immune precipitation. the material showed, upon polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, only four radioactive bands with molecular weights ranging from 17000 to 21000, which coincided well with interferon activity. they coincided also with the four interferon protein bands in the el ... | 1982 | 6180896 |
| detection of fungemia obscured by concomitant bacteremia: in vitro and in vivo studies. | our recent clinical experience suggested that bacteremia may interfere with the detection of concomitant fungemia when standard blood culture methods are used. to determine the extent to which bacteria may interfere with fungal isolation from blood cultures, an in vitro model simulating blood cultures taken during concomitant fungemia and bacteremia was created. each of six bacteria (staphylococcus epidermidis, staphylococcus aureus, streptococcus faecalis, escherichia coli, klebsiella pneumonia ... | 1982 | 6186687 |
| ifn-alpha-induced modulations of the events in human mixed lymphocyte cultures. | autologous mixed lymphocyte cultures (amc) with t-enriched subset of human blood lymphocytes as responders and b-cells or plastic adherent cells as stimulators and allogeneic mixed lymphocyte cultures (mlc) were assayed for blastogenesis and generation of cytotoxic potential. the activated cells lyzed k562 and daudi, autologous and allogeneic pha-blasts. the amc population affected the autologous and allogeneic blasts at a similar strength and there was no indication for selective effects. b-bla ... | 1982 | 6219067 |
| short-time low-temperature pasteurisation of human milk. | using the oxford human milk pasteuriser human milk was exposed to lower temperature and shorter time periods than employed in classical holder pasteurisation. heating human milk at 62.5 degrees c for 5 min completely destroyed escherichia coli, staphylococcus aureus, and group b beta-haemolytic streptococci inoculated into the samples. heating at 56.0 degrees c for 15 min destroyed over 99% of the inoculated organisms. the mean percentage remaining activity of certain antimicrobial proteins afte ... | 1982 | 6756884 |
| molecular organization of human l' and l pyruvate kinases. | extremities, peptide maps and phosphorylatable site localization of human erythrocyte l' and liver l pyruvate kinases (ec 2.7.1.40) were investigated. l' and l subunits seemed to have similar, blocked nh2 termini and differ in their sensitivity to carboxypeptidase a, that is to say in their c-terminal ends. after digestion by staphylococcus aureus v8 protease, the phosphorylated sites of both l' and l subunits were located on those peptides which were different in l' and l, that is to say on the ... | 1982 | 6758852 |
| isolation and characterization of circulating immune complexes in cystic fibrosis. | a methodology for the isolation and immunologic characterization of igg-containing circulating immune complexes (igg-cic) as detected by the 125iclq-binding assay (clqba) is described. we applied this methodology to sera from patients with cystic fibrosis (cf), both positive and negative for igg-cic. we used latex-fixation-positive rheumatoid arthritis sera and normal human sera as positive and negative controls, respectively. all igg-cic-positive serum samples from cf patients were found to con ... | 1982 | 6804143 |
| antimicrobial activity of topical anaesthetic preparations. | eight commercial topical anaesthetic preparations were tested for their ability to inhibit microbial growth in vitro by incubating serial dilutions with each of 4 micro-organisms. in addition corneas of mice were infected with staphylococcus aureus, and the effect of the anaesthetics on isolation rates of bacteria was investigated. the preparations were shown to have a wide range of antimicrobial activity, correlating both with the active agents and the preservatives. we suggest that some prepar ... | 1982 | 6805500 |
| labeling of rh antibodies on solid-phase protein a. | rh0(d) antibodies which retain immune specificity after radiolabeling were prepared by a procedure which does not require igg isolation from serum, requires 10-fold less isotope than conventional techniques and yields antibody solutions of defined composition. the method involves radioiodination of igg on immobilized protein a, depends on employing human red cells reduced in surface cytophilic igg, and exploits the inability of goat igg to interact with staphylococcus aureus protein a. the techn ... | 1982 | 6806390 |
| polyclonal response of human lymphocytes to bacterial cell walls, peptidoglycans and teichoic acids. | it has been found earlier that many bacteria are polyclonal activators of human lymphocytes. this phenomenon was further analysed by preparing cell walls, peptidoglycans and teichoic acids from staphylococcus aureus wood 46 and bacillus subtilis and studying their capacity to stimulate human adult and newborn lymphocytes to proliferate and to produce leucocyte inhibitory factor (lif). all these bacterial surface components acted as polyclonal activators. in our opinion these findings further str ... | 1982 | 6807836 |
| characterization of partially purified group b streptococcal clumping factor. | using a strain of group b streptococci, characteristics of the streptococcal clumping factor was investigated. in contrast to that of staphylococcus, the streptococcal clumping factor reacted with plasmas from guinea-pig and sheep although staphylococcal clumping factor did not react with them. in addition, streptococcal clumping factor was resistant to heat, sonication, and chemical reagents; it was also degradable by proteolytic enzyme and galactose oxidase. hydrochloric acid extract of the wh ... | 1982 | 6753418 |
| studies on bacterial hypersensitivity in man. interrelationship between skin reactions to bacterial peptidoglycan and serum peptidoglycan antibody titers. | the nature and extent of bacterially induced allergies are difficult to define. since peptidoglycan, the main component of the cell wall of almost all bacteria, has been available in a highly purified, chemically and immunologically well-defined form, investigation of the allergological significance of this cell component is feasible. intracutaneous tests were carried out on 181 test subjects with five different peptidoglycan (pg) preparations from staphylococcus aureus, staphylococcus epidermid ... | 1982 | 6753628 |
| polyclonal activation of human peripheral blood b lymphocytes by formaldehyde-fixed salmonella paratyphi b. ii. heterogeneity of b lymphocytes. | b-cell 'activation' in cultures stimulated with pokeweed mitogen (pwm), staphylococcus aureus strain cowan i, or formaldehyde-fixed salmonella paratyphi b (spb) was evaluated by enumeration of cells secreting immunoglobulin (ig) and by quantitation of ig released into culture supernatants. a dissociation between these two values was found after day 6 in cultures activated with pwm or spb, suggesting that ig-secreting cells (isc) are heterogeneous in terms of ig secretion rate. generation of isc ... | 1982 | 6979775 |
| protein a reactivity of lymphocytes from some patients with chronic lymphocytic leukaemia mediated by an interaction with the f(ab')2 region of surface immunoglobulin. | peripheral blood lymphocytes (pbl) from 15 of 38 patients with chronic lymphocytic leukaemia (cll) were capable of forming rosettes with human erythrocytes coated with staphylococcal protein a (spa-hrbc). pbl from seven patients also showed a marked proliferative response after stimulation with staphylococcus aureus bacteria strain cowan i (cowan staph). the spa-rosetting of cll cells was inhibited by incubation with f(ab')2 fragments of anti-immunoglobulin (ig) antibodies. in addition, incubati ... | 1982 | 6979776 |
| enhancement of human b lymphocyte differentiation in vitro by thyroid hormone. | a study was made of the regulatory influence of a thyroid hormone, triiodothyronine (t3), on human blood t- and b-lymphocytes responses in vitro. blood lymphocytes were stimulated with the t-cell mitogens concanavalin a (con a) and phytohaemagglutinin (pha), with the t-dependent b-cell activator pokeweed mitogen (pwm), and with the t-independent b-cell activator staphylococcus aureus cowan i bacteria in the presence of various hormone concentrations. t3 did not stimulate t-cell proliferation. th ... | 1982 | 6980446 |
| proliferative responses of normal human b lymphocytes. development of an assay system for human b cell growth factor (bcgf). | a simple and reproducible system for inducing and measuring proliferation of normal human peripheral blood b lymphocytes was developed and employed as an assay for bcgf activity contained in supernatants of cultured human mononuclear cells. sac was used to activate human peripheral blood b cells to develop into blasts with low levels of proliferation, and a marked synergistic effect on the proliferation of these activated b cell blasts was demonstrated when various culture-derived supernatants c ... | 1982 | 6980918 |
| specific serum ige antibodies to bacterial antigens in allergic lung disease. | a radio-allergosorbent test (rast) to measure specific ige antibodies in man to whole bacterial cells of streptococcus pneumoniae, staphylococcus aureus and haemophilus influenzae was developed to investigate different well-defined lung diseases (chronic bronchitis, allergic bronchopulmonary aspergillosis (abpa), bronchial asthma allergic rhinitis, cystic fibrosis) and also in urticaria as compared with non-atopic blood donors. in addition, total ige values and skin prick tests were assessed in ... | 1982 | 6982789 |
| reduced hemoglobin as an inhibitor of human polymorphonuclear leukocyte bacterial killing. role of hemoglobin--oxygen tension in polymorphonuclear function. | the effect of reduced hemoglobin (hb) on in vitro human polymorphonuclear leukocyte (pmnl) bactericidal activity was investigated. addition of hb at physiologic concentrations and oxygen partial pressures (150 mg/ml, 35 torr) to pmnl bactericidal assays significantly inhibited the killing of blood culture isolates of escherichia and staphylococcus aureus. raising the oxygen partial pressure above 35 torr greatly decreased this inhibition. carboxy hemoglobin, produced by equilibrium of hg with ca ... | 1982 | 7034261 |
| expression of treponema pallidum antigens in escherichia coli. | treponema pallidum dna was cloned in a bacteriophage. clones were screened for expression of treponema pallidum antigens by an in situ radioimmunoassay on nitrocellulose, with the use of subsequent reactions with syphilitic serum and radioiodinated staphylococcus aureus protein a. one clone, which gave a strong signal, codes for at least seven antigens that react specifically with human antibodies to treponema pallidum. | 1982 | 7041257 |
| isoelectric points and surface hydrophobicity of gram-positive cocci as determined by cross-partition and hydrophobic affinity partition in aqueous two-phase systems. | thirty-nine streptococcal strains belonging to groups a, c, and g and 12 staphylococcal strains were investigated with respect to surface charge and hydrophobicity. isoelectric points of the bacteria were determined by cross-partition experiments in dextran-polyethylene glycol two-phase systems containing charged polymers. the results obtained indicate that group a, c, and g streptococci have isoelectric points of ph 3.75 +/- 0.15 standard deviation. staphylococci show an isoelectric point of ar ... | 1982 | 7042571 |
| the primary structure of human erythrocyte copper/zinc superoxide dismutase: cleavage with staphylococcus aureus protease, determination of the n-terminal blocking group and location of the disulfide bond. | results obtained after digestion of copper/zinc superoxide dismutase from human erythrocytes with s. aureus protease are described. in particular, peptides soluble in alkaline conditions proved essential for completing the determination of the primary structure of the enzyme; other peptides were important for establishing the amidation state of dicarboxylic amino acid residues and for confirming controversial sequences. the human enzyme is acetylated at the nh2 terminus and contains an intrasubu ... | 1982 | 7045030 |
| in vitro evaluation of centa, a new beta-lactamase-susceptible chromogenic cephalosporin reagent. | centa is a newly synthesized, beta-lactamase-labile, chromogenic cephalosporin reagent which changes color from light yellow (lambda maximum ca. 340 nm) to chrome yellow (lambda maximum ca. 405 nm) concomitant with hydrolysis of the beta-lactam ring. this compound offers promise as a diagnostic reagent comparable to other chromogens (padac and nitrocefin) for the early detection of beta-lactamase-producing clinical isolates, while retaining some antimicrobial effect against escherichia coli, kle ... | 1982 | 7047560 |
| [possibilities of resuming breast feeding after suppurative or inflammatory breast diseases]. | 1982 | 7070913 | |
| characterization of the binding domains in the fragments cleaved by cathepsin g from human plasma fibronectin. | 1982 | 7075587 | |
| high-level potentiation of lysostaphin anti-staphylococcal activity by lysozyme. | the purpose of this study was to determine whether lysostaphin would enhance its anti-staphylococcal efficacy in combination with lysozyme. minimum inhibitory concentrations (mics) of lysostaphin and lysozyme were separately determined for 41 strains belonging to 10 different species of human staphylococci. lysozyme was virtually inactive and showed mics of 15 mg/ml. on the contrary, all strains were susceptible to lysostaphin and showed mics ranging from 2.5 to 60 micrograms/ml for the differen ... | 1982 | 7081976 |
| anti-poly(glycerolphosphate) in human sera. | ultrasonic extracts from staphylococci contained red cell-sensitizing poly(glycerolphosphate)teichoic acid antigen. the antigen may contribute to the variability of precipitin reactions used in the diagnosis of staphylococcus aureus infection. | 1982 | 7107847 |
| enterotoxin production by staphylococcus aureus strains isolated from humans, foods and animals in malaysia. | enterotoxin production by strains of staphylococcus aureus isolated from human, food and animal sources was investigated. of the 130 isolates studied, 27 (20.8%) were found to be enterotoxigenic. the most common enterotoxin detected from human sources was enterotoxin c whereas enterotoxin b occurred more frequently in staphylococcal strains of food origin. the 2 enterotoxigenic strains, from animals isolated from a dog and a goat, produced enterotoxins a and c, respectively. enterotoxin e was no ... | 1982 | 7112212 |
| fixed protein a-containing staphylococci as solid-phase immunoadsorbents. | staphylococcus aureus cowan i containing protein a were rapidly fixed in hot 5% (w/v) trichloroacetic acid (tca) for 6 min in order to remove the negatively charged cell wall polymer teichoic acid. this resulted in a stable igg adsorbent able to bind 1.4 mg of human igg/ml 10% (v/v) suspension for at least 5 months. this igg binding ability is 25% less than for formalin-fixed bacteria, which were stable for at least 1 year. preincubation of the bacterial adsorbents in 0.5% (v/v) tween 20 after f ... | 1982 | 7119455 |
| the effect of exposure of staphylococcus aureus to penicillin on susceptibility to the bactericidal activity of human leukocytes. | the effect of brief exposure of staphylococci to penicillin upon susceptibility to leukocidal activity was studied. antibiotic pretreated staphylococci were incubated with normal human leukocytes and viability of the bacteria was determined. penicillin pretreated staphylococci were more susceptible to killing by leukocytes than untreated control bacteria. the extent of sensitization varied between different strains of staphylococci. when staphylococci were exposed simultaneously to leukocytes an ... | 1982 | 7133758 |