Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| interaction between the helicobacter pylori caga and alpha-pix in gastric epithelial ags cells. | the gastric pathogen helicobacter pylori (h. pylori) translocates the caga protein into epithelial cells by a type iv secretion process. upon translocation into the host cell cytosol, caga undergoes tyrosine phosphorylation. phosphorylation of caga occurs within the c terminus of the protein and is mediated by members of the src family of tyrosine kinases. phosphorylation of caga induces the dephosphorylation of as yet unidentified cellular proteins, rearrangements of the host cell actin cytoske ... | 2007 | 17405911 |
| primary hepatic low-grade b-cell lymphoma of malt-type associated with helicobacter pylori infection. | primary marginal zone b-cell lymphoma of mucosa associated tissue (malt) type in the liver is extremely rare, and the etiology of this disease is yet to be clarified. we present the first report of a primary hepatic low-grade lymphoma of malt-type associated with helicobacter pylori (h. pylori) infection. a 64-year-old man was referred to our hospital for the treatment of early gastric carcinoma. he underwent distal gastrectomy with regional lymph node dissection. in the operation, several small ... | 2007 | 18019744 |
| [carbamazepine and clarithromycin: a clinically relevant drug interaction]. | carbamazepine is associated with clinically relevant drug interactions especially with macrolide antibiotics such as troleandomycin and erythromycin. these drugs inhibit the metabolism of carbamazepine. clarithromycin, a macrolide antibiotic similar to erythromycin, is widely used to treat respiratory tract infections and is used for the treatment of atypical mycobacterial infections and helicobacter pylori-associated peptic ulcer disease. | 2007 | 18033049 |
| evidence for cancer-associated expression of nadph oxidase 1 (nox1)-based oxidase system in the human stomach. | helicobacter pylori infection has been suggested to stimulate expression of the nadph oxidase 1 (nox1)-based oxidase system in guinea pig gastric epithelium, whereas nox1 mrna expression has not yet been documented in the human stomach. pcr of human stomach cdna libraries showed that nox1 and nox organizer 1 (noxo1) messages were absent from normal stomachs, while they were specifically coexpressed in intestinal- and diffuse-type adenocarcinomas including signet-ring cell carcinoma. immunohistoc ... | 2007 | 18037128 |
| association of toll-like receptor-4 (asp299gly and thr399ileu) gene polymorphisms with gastritis and precancerous lesions. | a toll-like receptor-4 (tlr-4) asp299gly and thr399ileu substitution reduces responsiveness to helicobacter pylori (h. pylori) lipopolysaccharide. caga+ strains of h. pylori are known to be associated with gastroduodenal diseases. therefore we aimed to evaluate association of tlr-4 substitutions and caga seropositivity with gastritis and precancerous lesions in a northern indian population. after upper gastrointestinal endoscopy, 130 rapid urease test (rut)-positive patients with nonulcer dyspep ... | 2007 | 18082569 |
| predictive role of nuclear factor-kappab activity in gastric cancer: a promising adjuvant approach with caffeic acid phenethyl ester. | the biologic significance of nuclear factor-kappab (nf-kappab) activation in human gastric cancer is unclear. we clarify the clinical significance of nf-kappab activation and its relationship to helicobacter pylori infection, a well-known pathogenesis of gastric cancer. moreover, we examine the effects and underlying mechanisms induced by caffeic acid phenethyl ester (cape), an inhibitor of nf-kappab, for gastric carcinoma. | 2007 | 18090157 |
| [a case of lung cancer with idiopathic thrombocytopenic purpura effectively treated for helicobacter pylori]. | a 58-year-old man with cough and bloody sputum was admitted because of right lung tumor and thrombocytopenia. idiopathic thrombocytopenic purpura (itp) was diagnosed. for treatment of itp, he was treated with platelet transfusions, intravenous gamma-globulin and oral prednisolone. however, the platelet count did not completely improved. since a relationship between itp and helicobacter pylori (hp) infection has been reported, and result of urease test was positive, the patient was treated by hp ... | 2007 | 18186249 |
| how to influence health providers. | in spite of the recognition that helicobacter pylori is responsible for most duodenal ulcer and peptic ulcer and that it is the underlying cause of most gastric cancer, little or no attention has been paid to its effect upon public health and in particular no measures have been put in place to address them. for over 20 years relatively inexpensive and noninvasive testing and treatment have been available, but no country has introduced a "screen-and-treat" policy, and relatively little research h ... | 2007 | 17991182 |
| probiotics and small bowel mucosa: molecular aspects of their interactions. | probiotics are described as "friendly bacteria" that could improve the intestine defense by interacting with the resident microflora. there is a large body of evidence suggesting that consumption of functional food containing probiotics exerts positive effects on human health. several clinical trials have highlighted the efficiency of probiotics in the prevention and treatment of different gastrointestinal disorders including the prevention of antibiotic associated diarrhea, the remission in pat ... | 2006 | 18850204 |
| gastric stump lymphoma after distal gastrectomy for benign peptic ulcer: report of a case. | we report a case of primary non-hodgkin gastric stump lymphoma, found in a 78-year-old man 30 years after a distal gastrectomy for a benign peptic ulcer. the development of lymphoma in the gastric stump is rare. in fact, to our knowledge only 37 cases, including this one, have been documented. although helicobacter pylori is thought to be a predisposing factor, we found no histological evidence of this infection in our patient. conversely, bile reflux and nitrite and n-nitrous compounds caused b ... | 2006 | 17072720 |
| interleukin-8 induction by helicobacter pylori in gastric epithelial cells is dependent on apurinic/apyrimidinic endonuclease-1/redox factor-1. | helicobacter pylori infection causes inflammation and increases the expression of il-8 in human gastric epithelial cells. h. pylori activates nf-kappab and ap-1, essential transcriptional factors in h. pylori-induced il-8 gene transcription. although colonization creates a local oxidative stress, the molecular basis for the transition from infection to the expression of redox-sensitive cytokine genes is unknown. we recently reported that the expression of apurinic/apyrimidinic endonuclease-1/red ... | 2006 | 17114472 |
| trials of novel 13c-urea-containing capsule for more economic and sensitive diagnosis of helicobacter pylori infection in human subjects. | to develop a 13c-urea-containing capsule for more economic and sensitive diagnosis of helicobacter pylori infection, the 13c-urea-containing capsules were prepared with various additives such as polyethylene glycol, microcrystalline cellulose, sodium lauryl sulfate and citric acid. their dissolution test and 13c-urea breath test in human volunteers were then performed. polyethylene glycol increased the initial dissolution rates of urea and difference delta 13c values from 13c-urea, while microcr ... | 2006 | 17121183 |
| molecular mechanisms involved in anti-inflammatory effects of proton pump inhibitors. | interleukin (il)-8 has been reported to participate in neutrophil infiltration in helicobacter pylori (h. pylori)-induced gastritis in humans. in this study, we investigated the anti-inflammatory actions beyond the suppression of acid secretion by proton pump inhibitors (ppi), such as omeprazole and lansoprazole, on il-8 production by gastric epithelial cells (mkn45) and human umbilical vein endothelial cells (huvec) and on the transendothelial migration of polymorphonuclear neutrophils (pmn). | 2006 | 17122965 |
| assay of human gastric mucin as a natural antibiotic against helicobacter pylori. | helicobacter pylori infects more than half of the world's population and is considered a leading cause of peptic ulcer and gastric carcinoma. although a large number of persons are infected with h. pylori, only a limited number of those infected (approximately 3%) develop peptic ulcers and gastric carcinoma. the progression of the disease is restricted by deeper portion of the gastric mucosa, and in many persons glandular atrophy appears to be prevented by mucins secreted in the deeper portion o ... | 2006 | 17116474 |
| age-dependent seroprevalence of antibodies against a helicobacter pylori-like organism and helicobacter pylori in commercially reared swine. | to determine the prevalence of antibodies against a swine-origin helicobacter pylori-like organism (hplo) and h pylori in conventionally reared swine. | 2006 | 17078751 |
| clustering of helicobacter pylori vaca in lipid rafts, mediated by its receptor, receptor-like protein tyrosine phosphatase beta, is required for intoxication in az-521 cells. | helicobacter pylori vacuolating cytotoxin, vaca, induces multiple effects on epithelial cells through different cellular events: one involves pore formation, leading to vacuolation, mitochondrial damage, and apoptosis, and the second involves cell signaling, resulting in stimulation of proinflammatory responses and cell detachment. our recent data demonstrated that vaca uses receptor-like protein tyrosine phosphatase beta (rptpbeta) as a receptor, of which five residues (qttqp) at positions 747 ... | 2006 | 17030583 |
| helicobacter pylori-induced atrophic gastritis progressing to gastric cancer exhibits sonic hedgehog loss and aberrant cdx2 expression. | the loss of sonic hedgehog is an early change that occurs in the mucosa prior to neoplastic transformation and correlates with the type of intestinal metaplasia. aberrant expression of cdx has also been shown to correlate with the development of intestinal metaplasia. | 2006 | 17209848 |
| initial 48-hour acid inhibition by intravenous infusion of omeprazole, famotidine, or both in relation to cytochrome p450 2c19 genotype status. | faster and stronger acid inhibition is required for the treatment of hemorrhage from peptic ulcers. we compared the effects of intravenous infusion regimens of a proton pump inhibitor (ppi) alone, a histamine 2 receptor antagonist (h2ra) alone, and the combination of a ppi with an h2ra on acid inhibition in the early postadministration phase in relation to cytochrome p450 (cyp) 2c19 genotype status. | 2006 | 17112810 |
| o-glycosylation of serum iga1 antibodies against mucosal and systemic antigens in iga nephropathy. | in iga nephropathy (igan), serum iga1 with abnormal o-glycosylation deposits in the glomerular mesangium. the underlying mechanism of this iga1 o-glycosylation abnormality is poorly understood, but recent evidence argues against a generic defect in b cell glycosyltransferases, suggesting that only a subpopulation of iga1-committed b cells are affected. for investigation of whether the site of antigen encounter influences iga1 o-glycosylation, the o-glycosylation of serum iga1 antibodies against ... | 2006 | 17093066 |
| interplay between helicobacter pylori and the immune system. clinical implications. | helicobacter pylori (h. pylori) is a gram-negative bacteria infecting more than 50% of human population. h. pylori selectively colonizes gastric mucosa and represents the major cause of gastroduodenal pathologies, such as gastric ulcer, autoimmune gastritis, gastric cancer and b cell lymphoma of mucosa associated lymphoid tissue (malt). in this review interplay between h. pylori and both innate and adaptive immune responses is discussed. the second part of this article presents current knowledge ... | 2006 | 17033103 |
| tlr4-dependent nf-kappab activation and mitogen- and stress-activated protein kinase 1-triggered phosphorylation events are central to helicobacter pylori peptidyl prolyl cis-, trans-isomerase (hp0175)-mediated induction of il-6 release from macrophages. | helicobacter pylori infection is associated with the local production of chemokines and cytokines, of which il-6 is overexpressed at the margin of gastric ulcer in h. pylori-positive gastritis. cells of the monocytic lineage are the major sources of il-6, and mononuclear cell infiltration in the lamina propria is characteristic of h. pylori-induced chronic infection. our study shows for the first time that a secreted peptidyl prolyl cis-, trans-isomerase, hp0175 elicits il-6 gene expression and ... | 2006 | 17114467 |
| helicobacter pylori vaca toxin promotes bacterial intracellular survival in gastric epithelial cells. | helicobacter pylori colonizes the gastric epithelium of at least 50% of the world's human population, playing a causative role in the development of chronic gastritis, peptic ulcers, and gastric adenocarcinoma. current evidence indicates that h. pylori can invade epithelial cells in the gastric mucosa. however, relatively little is known about the biology of h. pylori invasion and survival in host cells. here, we analyze both the nature of and the mechanisms responsible for the formation of h. p ... | 2006 | 17000720 |
| clinical application of pharmacogenetics in gastrointestinal diseases. | as knowledge of the human genome grows, there will be a direct impact on the management of specific diseases. within gastroenterology and hepatology, there has been a change in the understanding of how variations or mutations in genes involved in drug metabolism or disease pathophysiology affect response to therapy. this review discusses the application of clinical pharmacogenetics to the following diseases and disorders: inflammatory bowel disease, helicobacter pylori infections, gastroesophage ... | 2006 | 17020413 |
| effect of oral administration of bismuth compounds on campylobacter colonization in broilers. | bismuth compounds have been used since the 18th century to treat gastrointestinal ailments in man. colloidal bismuth subcitrate (de-nol) is currently used in combination with antibiotics to reduce enteric helicobacter pylori colonization as a treatment of stomach ulcers. we investigated whether bismuth citrate or its parent compound, colloidal bismuth subcitrate, would reduce colonization of the closely related foodborne pathogen, campylobacter jejuni in chickens. in 2 studies, birds were either ... | 2006 | 17032837 |
| a population genetics pedigree perspective on the transmission of helicobacter pylori. | the inference of transmission pathways for medicinally important bacteria is important to our understanding of pathogens. here we report analyses of transmission in helicobacter pylori, a major carcinogen. our study is novel in that the focal community comprises detailed family pedigrees and has a high prevalence of h. pylori. to infer transmission, we performed high-resolution analyses of nucleotide sequences for three genes and accounted for the occurrence of mutation and recombination through ... | 2006 | 17057244 |
| bioinformatic analysis of helicobacter pylori xgprtase: a potential therapeutic target. | xanthine-guanine phosphoribosyltransferase (xgprtase) is an enzyme of purine nucleotide salvage synthesis. the gpt gene of helicobacter pylori has been annotated as encoding an xgprtase and proposed as essential for survival of the bacterium in vitro. the aims of this work were to investigate the structure of h. pylori xgprtase and to compare the key features of the enzyme to other phosphoribosyltransferases employing computational, modelling, and bioinformatic tools. | 2006 | 16882332 |
| human milk vacuolating cytotoxin a immunoglobulin a antibodies modify helicobacter pylori infection in gambian children. | we collected data, including the weights, urea breath test results, and presence of maternal milk cytotoxin-associated gene-specific and vacuolating cytotoxin a-specific immunoglobulin a monthly from 48 mothers and infants (to 44 weeks of age) in the gambia. in all, 11 children (23%) had negative urea breath test results, and 37 (77%) had positive results. weight loss associated with helicobacter pylori colonization was restricted to children whose mothers did not produce anti-vacuolating cytoto ... | 2006 | 16983617 |
| helicobacter pylori induces mono-(adenosine 5'-diphosphate)-ribosylation in human gastric adenocarcinoma. | mono-(adenosine 5'-diphosphate) (adp)-ribosylation, which transfers an adp-ribose from nicotinamide adenine dinucleotide (nad) to an acceptor protein, is an important post-translational modification of cellular proteins. several bacterial toxins are known to possess the mono-adp-ribosyltransferase activity to catalyze this reaction as a possible pathogenic factor. therefore, the aim of this study was to examine whether h. pylori may also induce mono-adp-ribosylation in a human gastric mucosal pr ... | 2006 | 16964392 |
| neuroimmune link in the mucosa of chronic gastritis with helicobacter pylori infection. | it is suggested that different neuropeptides regulate gastric mucosal integrity and participate in the development of chronic gastritis. the aim of this study was to examine the roles and changes of immunoreactive (ir) nerves and immunocompetent cells in human gastritis. immunohistochemical, immunocytochemical, and confocal laser microscopic methods were used. all investigated nerve fibers were found in different quantities in the mucosa of both control and gastritis samples. the number of sp, n ... | 2006 | 16983504 |
| cytotoxic 5-aryl-1-(4-nitrophenyl)-3-oxo-1,4-pentadienes mounted on alicyclic scaffolds. | the 5-aryl-1-(4-nitrophenyl)-3-oxo-1,4-pentadienyl pharmacophore was incorporated into four series of compounds 1-4. compounds 1a-g comprised a cluster of 3-arylidene-1-(4-nitrophenylmethylene)-2-oxo-3,4-dihydro-1h-naphthalenes while the analogues 2a-g consisted of a group of 6-arylidene-2-(4-nitrophenylmethylene)cyclohexanones. three other compounds prepared in this study were 1-(4-nitrophenylmethylene)-3-(3,4,5-trimethoxyphenylmethylene)-2-oxo-2,3-dihydro-1h-indene 3a as well as two 5-aryliden ... | 2006 | 16581158 |
| a case of cutaneous t cell pseudolymphoma in a patient with helicobacter pylori infection. | cutaneous pseudolymphomas (cpl) are benign cutaneous lymphoproliferative infiltrations of various origin, including among others bacterial infections, viral infections and drugs . helicobacter pylori has been frequently founded in the stomach of patients with malt lymphoma. in january 2001, a 43-year-old man was referred to our department because of a 1-month history of itchy erythematous patches, plaques and flat tumors on his body. histological examination revealed nodular infiltrations compos ... | 2006 | 16902296 |
| function and recruitment of mucosal regulatory t cells in human chronic helicobacter pylori infection and gastric adenocarcinoma. | cd4(+)cd25(high) foxp3-expressing regulatory t cells (treg) can suppress immune responses to infections and tumors, thereby promoting microbial persistence and tumor progression. however, little is known about the phenotype and function of human mucosal treg. therefore, we analyzed the suppressive activity and homing phenotype of treg in gastric mucosa of helicobacter pylori-infected gastric adenocarcinoma patients. we found increased numbers of cd4(+)foxp3(+) treg in the tumor compared to tumor ... | 2006 | 16934529 |
| h pylori receptor mhc class ii contributes to the dynamic gastric epithelial apoptotic response. | to investigate the role of mhc class ii in the modulation of gastric epithelial cell apoptosis induced by h pylori infection. | 2006 | 16937440 |
| urokinase system expression in gastric carcinoma: prognostic impact in an independent patient series and first evidence of predictive value in preoperative biopsy and intestinal metaplasia specimens. | the prognostic relevance of urokinase-type plasminogen activator (u-pa), u-pa receptor (u-par), and plasminogen activator inhibitor 1 (pai-1) in gastric carcinoma was demonstrated in an independent patient series. to the authors' knowledge,the roles of these activators as predictors of aggressive phenotypes in preoperative biopsies, helicobacter pylori infection, and intestinal metaplasia have to date not been investigated simultaneously in resected tumors. the objectives of the current study we ... | 2006 | 16435385 |
| preferential expression of cyclooxygenase-2 in colonic-phenotype of gastric intestinal metaplasia: association with helicobacter pylori and gastric carcinoma. | gastric intestinal metaplasia (gim) associated with h. pylori (hp) has been considered a premalignant lesion. however, gim phenotype associated with hp infection and gastric cancer is unclear. the expression of cox-2 in relation to gim phenotype is also unknown. | 2006 | 16394872 |
| carbonic anhydrase inhibitors: cloning and sulfonamide inhibition studies of a carboxyterminal truncated alpha-carbonic anhydrase from helicobacter pylori. | a library of sulfonamides/sulfamates has been investigated for the inhibition of the carboxyterminal truncated form of the alpha-carbonic anhydrase (ca, ec 4.2.1.1) isolated from the gastric pathogen helicobacter pylori (hpca). this enzyme, incorporating 202 amino acid residues, showed a catalytic activity similar to that of the full length hpca, with k(cat) of 2.35 x 10(5)s(-1) and k(cat)/k(m) of 1.56 x 10(7)m(-1)s(-1) at 25 degrees c and ph of 8.9, for the co(2) hydration reaction. all types o ... | 2006 | 16459077 |
| secretory leukocyte protease inhibitor expression in various types of gastritis: a specific role of helicobacter pylori infection. | secretory leukocyte protease inhibitor (slpi) represents a multifunctional protein of the gastrointestinal mucosa exerting antimicrobial and anti-inflammatory effects. slpi expression is generally induced during inflammation; however, helicobacter pylori-mediated gastritis is associated with significantly decreased antral slpi levels. the aim of the study was to investigate whether slpi downregulation of gastric mucosa represents a specific phenomenon of h. pylori infection or is generally linke ... | 2006 | 16462541 |
| helicobacter pylori diagnosis and management. | h pylori is a global human pathogen and is the major cause of gastritis and the gastritis-associated diseases: gastric ulcer, duodenal ulcer, gastric cancer, and primary gastric b-cell lymphoma (maltoma). although several reliable diagnostic tests are widely available, the ideal regimen for treating the infection re-mains to be established. the current first-line or legacy triple therapy regimens fail in 20% to 40% of patients. causes of treatment failure include antibiotic resistance, poor comp ... | 2006 | 16880064 |
| helicobacter pylori eradication therapy on histologic change in the distal esophagus. | although cases of reflux esophagitis (re) developing after treatment to eradicate helicobacter pylori have been discussed in some detail, no reports are available concerning the histologic examination of re both before and after eradication therapy. | 2006 | 16882323 |
| helicobacter pylori regulates the expression of inhibitors of dna binding (id) proteins by gastric epithelial cells. | id transcription factors control proliferation, differentiation and apoptosis by inhibiting the dna binding of basic helix-loop-helix transcription factors. increased expression of id proteins promotes proliferation, inhibits differentiation, and is associated with intestinal tumorigenesis. we aimed to determine how helicobacter pylori may alter the expression of id proteins by gastric epithelial cells: it was hypothesised that h. pylori, a known carcinogen, would result in increased expression ... | 2006 | 16473539 |
| mannan-binding lectin in children with chronic gastritis. | the involvement of mannan-binding lectin (mbl) insufficiency in the pathogenesis of chronic gastritis (cg) in children was investigated. blood samples were collected from 78 paediatric patients suffering from cg associated with helicobacter pylori infection (group hp(+)) and from 41 with the disease not associated with such an infection (group hp(-)). control group consisted of 77 children. the frequency of mbl-2 gene mutations and serum protein concentrations did not differ significantly in bot ... | 2006 | 16476012 |
| helicobacter pylori inhibits expression of heat shock protein 70 (hsp70) in human epithelial cell line. importance of cag a protein. | heat shock proteins (hsp) are crucial for the maintenance of cell integrity under normal cell growth and at pathophysiological conditions such as colonization of gastric mucosa by helicobacter pylori (hp). the effect of hp on mrna expression for hsp70 in the gastric epithelial cells in vitro has been little studied and remains inconclusive. in this study we attempted to determine the alterations in gene expression for hsp70 induced by two live strains of hp in the epithelial mkn7 cells. the foll ... | 2006 | 16845230 |
| expression of b7-h1 on gastric epithelial cells: its potential role in regulating t cells during helicobacter pylori infection. | helicobacter pylori infection is associated with gastritis, ulcers, and gastric cancer. the infection becomes chronic as the host response is unable to clear it. gastric epithelial cells (gec) play an important role during the host response, and their expression of class ii mhc and costimulatory molecules such as cd80 and cd86 suggests their role in local ag presentation. although t cells are recruited to the infected gastric mucosa, they have been reported to be hyporesponsive. in this study, w ... | 2006 | 16493058 |
| the pak1 autoregulatory domain is required for interaction with nik in helicobacter pylori-induced nf-kappab activation. | helicobacter pylori, the etiological agent of various human gastric diseases, induces the transcription factor nuclear factor kappab (nf-kappab) and proinflammatory cytokines/chemokines. we have characterised the direct interaction between p21-activated kinase 1 (pak1) and nf-kappab-inducing kinase (nik) in h. pylori-infected epithelial cells. the dimerisation (di) motif, which is part of the nh2-terminal autoregulatory domain of pak1, is critical for this interaction, whereas nik forms complexe ... | 2006 | 16497167 |
| hla-dqb1 locus and gastric cancer in helicobacter pylori infection. | it has been suggested that the incidence of digestive diseases associated with helicobacter pylori is influenced by the strain diversity of h. pylori, factors involving the host or environment, and the duration of infection. the authors have previously reported that human leukocyte antigen (hla)-dqb1*0401 plays an important role in the development of atrophic gastritis in h. pylori infected patients. the aim of the present study was to investigate the relationship between hla-dqb1 genotype and c ... | 2006 | 16509868 |
| effects of helicobacter pylori infection on mucin expression in gastric carcinoma and pericancerous tissues. | helicobacter pylori infection is one of the major causes of human gastric carcinoma and can disturb the gastric mucosa barrier. mucins have not only lubricating and protecting functions, but are also related to signal transduction, turnover of gastric epithelium and carcinogenesis of gastric mucosa. the aim of this study was to investigate the relationship between h. pylori infection and aberrant mucin expression in patients with gastric carcinoma. | 2006 | 16509869 |
| nucleotide-binding oligomerization domain-1 and epidermal growth factor receptor: critical regulators of beta-defensins during helicobacter pylori infection. | host-pathogen interactions that allow helicobacter pylori to survive and persist in the stomach of susceptible individuals remain unclear. human beta-defensins (hbds), epithelial-derived antimicrobial peptides are critical components of host-defense at mucosal surfaces. the role of h. pylori-mediated nf-kappab and epidermal growth factor receptor (egfr) activation on beta-defensin expression was investigated. transient transfection studies utilizing beta-defensin promoter constructs were conduct ... | 2006 | 16513653 |
| [development and clinical application of invader assay--detection of resistant mutation to clarithromycin in helicobacter pylori]. | invader assay is a novel technology used for the direct analysis of genomic dna without pcr amplification, including the detection of single nucleotide polymorphisms (snps) in human genome. it has high accuracy and reproducibility. we have previously developed snps assays of cytochrome p450 (cyp) 2c19 and many other drug metabolized enzymes by invader technology to realize personalized medicine. here, we developed invader assays to detect mutations with resistance to clarithromycin (cam) in h. p ... | 2006 | 16548239 |
| detection of lactobacillus gasseri oll2716 strain administered with yogurt drink in gastric mucus layer in humans. | in animal models and human trials, lactobacillus gasseri oll2716 (lg21) strain suppressed helicobacter pylori colonization in the stomach. the aim of the present study was to clarify whether orally administered lg21 strain can enter the gastric mucus layer. biopsy samples were taken from the gastric antrum and corpus of two healthy volunteers (h. pylori infected and non-infected) who drank yogurt supplemented with lg21 strains. dna of lg21 and h. pylori in the mucus layer was detected using the ... | 2006 | 17032235 |
| correlation of epstein-barr virus and its encoded proteins with helicobacter pylori and expression of c-met and c-myc in gastric carcinoma. | to investigate the interrelationship of epstein-barr virus (ebv) and ebv- encoded proteins with helicobacter pylori (h pylori) infection and the expression of c-met and c-myc oncogene proteins in gastric carcinoma, and to explore their role in gastric carcinogenesis. | 2006 | 16609989 |
| the stringent response is required for helicobacter pylori survival of stationary phase, exposure to acid, and aerobic shock. | the gastric pathogen helicobacter pylori must adapt to fluctuating conditions in the harsh environment of the human stomach with the use of a minimal number of transcriptional regulators. we investigated whether h. pylori utilizes the stringent response, involving signaling through the alarmone (p)ppgpp, as a survival strategy during environmental stresses. we show that the h. pylori homologue of the bifunctional (p)ppgpp synthetase and hydrolase spot is responsible for all cellular (p)ppgpp pro ... | 2006 | 16855239 |
| role of toll-like receptors in health and diseases of gastrointestinal tract. | the human gastrointestinal (gi) tract is colonized by non-pathogenic commensal microflora and frequently exposed to many pathogenic organisms. for the maintenance of gi homeostasis, the host must discriminate between pathogenic and non-pathogenic organisms and initiate effective and appropriate immune and inflammatory responses. mammalian toll-like receptors (tlrs) are members of the pattern-recognition receptor (prr) family that plays a central role in the initiation of innate cellular immune r ... | 2006 | 16610014 |
| [expression and immunogenicity analysis of a recombinant fusion protein of v. cholera ctb and h. pylori ure i]. | to express fusion protein of the cholera toxin b subunit (ctb) and the urea membrane channel gene (ure i) of h. pylori in e. coli, and analyze its immunogenicity. | 2006 | 16643777 |
| the infection connection. helicobacter pylori is more than just the cause of gastric ulcers--it offers an unprecedented opportunity to study changes in human microecology and the nature of chronic disease. | 2006 | 16670677 | |
| differential caspase-3 expression in noncancerous, premalignant and cancer tissues of stomach and its clinical implication. | caspase-3 is a critical apoptosis-promoting element but its status during stepwise gastrocarcinogenesis needs to be further clarified. | 2006 | 16697119 |
| erythrocyte lewis (a+b-) host phenotype is a factor with familial clustering for increased risk of helicobacter pylori-related non-cardiac gastric cancer. | the purpose of the present study was to test whether host erythrocyte lewis phenotypes correlated with the risk of gastric cancers. because of the association of gastric cancer with familial clustering, cancer relatives were investigated as to whether they had unique distribution of lewis phenotypes. | 2006 | 16724994 |
| oxidative phosphorylation and its coupling to mitochondrial creatine and adenylate kinases in human gastric mucosa. | energy metabolism in gastrobiopsy specimens of the antral and corpus mucosa, treated with saponin to permeabilize the cells, was studied in patients with gastric diseases. the results show twice lower oxidative capacity in the antral mucosa than in the corpus mucosa and the relative deficiency of antral mitochondria in complex i. the mucosal cells expressed mitochondrial and cytosolic isoforms of creatine kinase and adenylate kinase (ak). creatine (20 mm) and amp (2 mm) markedly stimulated mitoc ... | 2006 | 16741143 |
| gene-expression profiles in gastric epithelial cells stimulated with spiral and coccoid helicobacter pylori. | human gastric epithelial immortalized ges-1 cells were infected with spiral and coccoid helicobacter pylori. scanning electron microscopy was used to determine the ability of the two forms of h. pylori to adhere to ges-1 cells. ges-1 cell apoptosis induced by coccoid and spiral h. pylori was analysed using flow cytometry. a cdna microarray for 22,000 human genes was used to identify the gene-expression differences in ges-1 cells infected with the two forms of h. pylori, and the gene expression i ... | 2006 | 16849720 |
| demonstration and characterization of mutations induced by helicobacter pylori organisms in gastric epithelial cells. | helicobacter pylori gastritis increases gastric cancer risk. microsatellite instability-type mutations are secondary to deficient dna mismatch repair. h. pylori gastritis is more frequent in patients with microsatellite instability-positive gastric cancers, and h. pylori organisms independently of inflammation can reduce dna mismatch repair protein levels, raising the hypothesis that h. pylori organisms might lead to mutagenesis during infection. | 2006 | 16882331 |
| should the presence of polymorphisms of cyp2c19 enzymes influence the choice of the proton pump inhibitor for treatment of helicobacter pylori infection? | there are variations in the cyp2c19 genotypes, that are important for the metabolism of ppis. patients who are heterozygotes for the mutation, but especially homozygotes, have a much slower metabolism, which will result in more profound acid suppression. studies have been published, that suggest that the success rate of anti-helicobacter therapy is in part related to the cyp2c19 genotype of the patient. however, it is important to keep in mind that most studies that have evaluated this have been ... | 2006 | 16863548 |
| natural mannose-binding lectin (mbl) down-regulates phagocytosis of helicobacter pylori. | considering the role of lectin-carbohydrate interactions between helicobacter pylori bacteria and the host cells we addressed the question on how mannose binding lectin - mbl, present in human plasma, may influence the phagocytosis of h. pylori by peripheral blood granulocytes. for phagocytosis assay the granulocytes separated from peripheral blood of healthy h. pylori-seronegative donors were used. phagocytosis was estimated by fluorescence assay using fitc-labelled h. pylori cells. the mbl lev ... | 2006 | 17419286 |
| helicobacter pylori eradication induces marked increase in h+/k+-adenosine triphosphatase expression without altering parietal cell number in human gastric mucosa. | gastric acid secretion is downregulated by helicobacter pylori infection and upregulated after its eradication, but the mechanisms are still unclear. we examined the effects of h pylori eradication on the number of parietal cells and on expression of molecules functioning in acid secretion in the human gastric mucosa. | 2006 | 15872000 |
| influence of antigen on the development of malt lymphoma. | mucosa-associated lymphoid tissue (malt) b-cell lymphomas develop in the context of autoimmune or chronic inflammations like helicobacter pylori-induced gastritis. remission of most gastric malt lymphomas after eradication of h pylori links tumor cell proliferation to antigen-induced inflammation and the need for antigenic contact. furthermore, the tumor cells correspond to antigen-activated memory b cells. to investigate the reactivity of the tumor immunoglobulins we employed in vitro-generated ... | 2006 | 16204314 |
| association between serum pepsinogens and polymorphismof ptpn11 encoding shp-2 among helicobacter pylori seropositive japanese. | helicobacter pylori (h. pylori) plays a crucial role in the development of gastric atrophy and cancer, and caga-positive strains, which are universal in japan, increase the risk of these outcomes substantially. the caga protein is injected from attached h. pylori into gastric epithelial cells and undergoes src-dependent tyrosine phosphorylation and activation of the eukaryotic phosphatase shp-2. the caga/shp-2 interactions elicit cellular changes that increase the risk of carcinogenesis. we inve ... | 2006 | 16032704 |
| nickel represses the synthesis of the nickel permease nixa of helicobacter pylori. | nickel acquisition is necessary for urease activity, a major virulence factor of the human gastric pathogen helicobacter pylori. nixa was identified as a specific nickel uptake system in this organism. addition of small amounts of nickel to media strongly stimulates urea hydrolysis. on the other hand, high nickel concentrations are deleterious to cell growth. as a possible protective reaction, nickel uptake seems to be reduced in h. pylori grown in nickel-rich media. these observations led to in ... | 2006 | 16452405 |
| helicobacter felis and helicobacter bizzozeronii induce gastric parietal cell loss in mongolian gerbils. | non-pylori helicobacter infections are associated with gastritis, gastric ulcers and malt lymphomas in man. approximately 50% of these are caused by helicobacters commonly found in dogs and cats, including helicobacter felis, helicobacter bizzozeronii and h. salomonis. in contrast to helicobacter pylori, the virulence mechanisms of these species are unknown. in this study the virulence of h. felis, h. bizzozeronii and h. salomonis was investigated in mongolian gerbils. female spf gerbils were in ... | 2006 | 16311055 |
| association between herpes simplex virus type 1 and helicobacter pylori in us adolescents. | to understand how helicobacter pylori infection is acquired and the role that herpes simplex virus type 1 (hsv-1) may have, we determined whether an association between hsv and h pylori exists at the individual level and for what reason. | 2006 | 16360609 |
| signalling pathways and molecular interactions of nod1 and nod2. | the nod (nucleotide-binding oligomerization domain) proteins nod1 and nod2 have important roles in innate immunity as sensors of microbial components derived from bacterial peptidoglycan. the importance of these molecules is underscored by the fact that mutations in the gene that encodes nod2 occur in a subpopulation of patients with crohn's disease, and nod1 has also been shown to participate in host defence against infection with helicobacter pylori. here, we focus on the molecular interaction ... | 2006 | 16493424 |
| bacteria and cancer: cause, coincidence or cure? a review. | research has found that certain bacteria are associated with human cancers. their role, however, is still unclear. convincing evidence links some species to carcinogenesis while others appear promising in the diagnosis, prevention or treatment of cancers. the complex relationship between bacteria and humans is demonstrated by helicobacter pylori and salmonella typhi infections. research has shown that h. pylori can cause gastric cancer or malt lymphoma in some individuals. in contrast, exposure ... | 2006 | 16566840 |
| the cell-specific phenotype of the polymorphic vaca midregion is independent of the appearance of the cell surface receptor protein tyrosine phosphatase beta. | there are two alleles, m1 and m2, of the midregion of the vacuolating cytotoxin gene (vaca) of helicobacter pylori which code for toxins with different cell specificities. here we describe the construction of five chimeric strains in which regions of vaca were exchanged between the two genotypes. by analyzing the toxicity of these strains for hela and rk13 cells we have confirmed that a 148-amino-acid region determines the phenotypic differences between the two forms of the protein and that this ... | 2006 | 16368956 |
| the global health burden of infection-associated cancers in the year 2002. | several infectious agents are considered to be causes of cancer in humans. the fraction of the different types of cancer, and of all cancers worldwide and in different regions, has been estimated using several methods; primarily by reviewing the evidence for the strength of the association (relative risk) and the prevalence of infection in different world areas. the estimated total of infection-attributable cancer in the year 2002 is 1.9 million cases, or 17.8% of the global cancer burden. the p ... | 2006 | 16404738 |
| disposition of exogenous urea and effects of diet in rats. | although breath test using 13c-labeled urea (cas 57-13-6, ubt) is becoming popular for the diagnosis of helicobacter pylori (h. pylori) infection, disposition of exogenously given urea is not fully understood. the purpose of the present study is to elucidate the disposition of exogenous urea and to consider its relation with the ubt safety and biobehavior of endogenous urea. with 14c-labeled urea ([14c]urea), the absorption, distribution, metabolism and excretion including that into breathed air ... | 2006 | 16618019 |
| epstein-barr virus (ebv) infection and p53 protein expression in gastric carcinoma. | in the presented studies p53 protein expression was evaluated in samples of gastric carcinoma originating from 32 selected adult patients (with documented diagnosis of adenocarcinoma of the stomach and without the presence of helicobacter pylori infection). among the patients 14 individuals carried ebv-positive gastric carcinoma (group 1) while the 18 remaining patients carried ebv-negative gastric carcinoma (group 2). ebv infection was detected testing the tissue material for the presence of eb ... | 2006 | 16413625 |
| helicobacter pylori has stimulatory effects on naive t cells. | despite an apparently active host response, helicobacter pylori infection can persist for life. unexpectedly, t cells from apparently uninfected individuals respond to h. pylori antigen by proliferating. also, the t-cell proliferative response appears to be less in infected compared with uninfected individuals. | 2006 | 16423086 |
| antibacterial and antioxidant activities in sideritis italica (miller) greuter et burdet essential oils. | sideritis italica (miller) greuter et burdet is a widespread lamiacea in the mediterranean region used in traditional medicine. essential oils were antibacterial against nine atcc and as many clinically isolated gram-positive and gram-negative bacterial strains. antibacterial activity was also found against helicobacter pylori: a dose-dependant inhibition was shown between 5 and 25 microg/ml. the antibacterial activity of the oils was expressed as mics (minimum inhibitory concentrations) and mbc ... | 2006 | 16690235 |
| herpes simplex virus type 1 in peptic ulcer disease: an inverse association with helicobacter pylori. | to assess the frequency of herpes simplex virus type i in upper gastrointestinal tract ulcers and normal mucosa with the modern and better assays and also with a larger number of well characterized patients and controls and its relationship to helicobacter pylori(h pylori). | 2005 | 16425358 |
| [long-term follow-up of h. pylori infection--from gastritis to gastric cancer]. | 2005 | 16363529 | |
| [recent topics on important drugs for h. pylori eradication: omeprazole]. | 2005 | 16363556 | |
| [relationship between helicobacter pylori caga-shp-2 interaction and gastric cancer]. | 2005 | 16363503 | |
| [concomitant dosing of a h2 receptor antagonist (h2ra) with a triple therapy increases the cure rate of helicobacter pylori infection]. | 2005 | 16363566 | |
| [long-term outcome in patients with gastric malt lymphoma after h. pylori eradication]. | 2005 | 16363592 | |
| [influence of cytochrome p450 2c19 genetic polymorphism on h. pylori eradication therapy]. | 2005 | 16363603 | |
| [immune and inflammatory reaction to helicobacter pylori in the stomach]. | 2005 | 16363522 | |
| [transmission route of h. pylori]. | 2005 | 16363526 | |
| interleukin-8, cyclo-oxygenase-2, and trefoil factor family 1 gene expression and their association with helicobacter pylori infection in the remnant stomach. | the risk factors for secondary stomach carcinogenesis after distal gastrectomy have not been evaluated in detail. | 2005 | 16341482 |
| no correlation of baba2 with vaca and caga genotypes of helicobacter pylori and grading of gastritis from peptic ulcer disease patients in brazil. | the baba2 gene, which encodes a blood-group antigen-binding adhesin that mediates attachment of helicobacter pylori to human lewis(b) antigens on gastric epithelial cells, has been associated with a higher risk of peptic ulcer and gastric cancer. the purpose of this study was to ascertain the frequency of baba2 genotype in h. pylori strains of patients with peptic ulcer and to correlate with other virulence factors. | 2005 | 16302986 |
| role of surfactant protein d (sp-d) in innate immunity in the gastric mucosa: evidence of interaction with helicobacter pylori lipopolysaccharide. | surfactant protein d (sp-d) is a collagenous glycoprotein, a collectin, which functions as a pathogen-associated molecular pattern (pamp) recognition receptor in the innate immune response. although originally identified in the lung as a component of surfactant, sp-d also occurs in the gastric mucosa at the luminal surface and within gastric pits of mucus-secreting cells. infection with the gastroduodenal pathogen helicobacter pylori up-regulates expression of sp-d in human patients with gastrit ... | 2005 | 16303091 |
| molecular cloning and functional expression of a novel helicobacter pylori alpha-1,4 fucosyltransferase. | helicobacter pylori is an important human pathogen which causes both gastric and duodenal ulcers and is associated with gastric cancer and lymphoma. this microorganism synthesizes fucosylated oligosaccharides, predominantly the galb-1,4glcnac (type ii) blood group antigens lewis x and y, whereas a small population also expresses the galb-1,3glcnac (type i) blood group antigens lewis a and b. these carbohydrate structures are known to mimic host cell antigens and permit the bacteria to escape fro ... | 2005 | 16000696 |
| increased risk of intestinal type of gastric adenocarcinoma in japanese women associated with long forms of ccttt pentanucleotide repeat in the inducible nitric oxide synthase promoter. | tandem repeat number polymorphism of a ccttt pentanucleotide in the promoter region of the inducible nitric oxide synthase gene (inos) and a polymorphism of the interleukin-1beta (il-1b) promoter at position -31 were analyzed in dna samples from 181 japanese control subjects and 158 gastric cancer patients, including 96 intestinal type and 62 diffuse type. an association between the intestinal type of gastric adenocarcinoma and higher promoter activity of the inos gene was found in women, especi ... | 2005 | 15617837 |
| suppressive effects of fruit-juice concentrate of prunus mume sieb. et zucc. (japanese apricot, ume) on helicobacter pylori-induced glandular stomach lesions in mongolian gerbils. | helicobacter pylori (hp) infection is an important factor in human gastric disorders, including chronic active gastritis, peptic ulcers, intestinal metaplasia and cancer. since epidemiologic studies overwhelmingly agree on a protective influence of fruits and vegetables in reducing the risk of gastric neoplasia and processed foods made from prunus mume sieb. et zucc. (japanese apricot or "ume" in japanese) are traditionally known for their miscellaneous medical effects, in the present study we i ... | 2005 | 16235996 |
| helicobacter pylori-stimulated interleukin-8 (il-8) promotes cell proliferation through transactivation of epidermal growth factor receptor (egfr) by disintegrin and metalloproteinase (adam) activation. | helicobacter pylori infection increases the risk of hyperplastic polyps and gastric cancer, but the mechanisms remain to be elucidated. h. pylori was recently shown to transactivate epidermal growth factor receptor (egfr) through metalloprotease stimulation. the present study was designed to investigate the effect of interleukin-8 (il-8) induced by h. pylori infection on egfr transactivation and epithelial cell growth. h. pylori sydney strain 1 (ss1) having wild-type cag(+)a was used. phospho-eg ... | 2005 | 16240219 |
| upregulation of cathepsin w-expressing t cells is specific for autoimmune atrophic gastritis compared to other types of chronic gastritis. | to investigate a pathophysiological role of cathepsin w (catw), a putative thiol-dependent cysteine protease, which is specifically expressed in cytotoxic lymphocytes, in different types of chronic inflammation of the gastric mucosa. | 2005 | 16273605 |
| [cytochrome p450 2c19 genotype -based second line therapy with amoxicillin and lansoprazole after failure of initial helicobacter pylori eradication therapy]. | 2005 | 16416832 | |
| multiplex-touchdown pcr assay for the detection and genotyping of helicobacter pylori from artificially contaminated sheep milk. | helicobacter pylori (hp) is an organism commonly present worldwide in the human population, sometimes causing serious illnesses such as duodenal and gastric ulcers, adenocarcinoma of the stomach, and low-grade b-cell mucosa-associated lymphoid tissue lymphoma of the stomach. this article describes a multiplex-touchdown pcr method for the identification and genotyping (vaca-s1/m1, sl/m2, and s2/m2-and caga genes) of hp directly from sheep milk artificially contaminated with hp strains from human ... | 2005 | 16245719 |
| is the hypoxia-inducible factor pathway important in gastric cancer? | tumour hypoxia is well recognised in oncology to be a key factor resulting in treatment resistance and poor prognosis. hypoxia leads to the expression of a number of gene products that are involved in tumour progression, invasion and metastasis formation. the most important of these proteins is thought to be hypoxia-inducible factor-1alpha (hif-1alpha), which appears to be a master regulator of the cellular response to hypoxia. hif-1alpha expression is associated with a poor prognosis and treatm ... | 2005 | 16290133 |
| [helicobacter pylori caga and shp-2 tyrosine phosphatase]. | 2005 | 16296318 | |
| synergism of helicobacter pylori infection and stress on the augmentation of gastric mucosal damage and its prevention with alpha-tocopherol. | despite evidence that helicobacter pylori (h. pylori) infection is closely associated with stress in gastric ulcer patients, the underlying mechanism why ulcer recurrence after stress is augmented especially in patients with h. pylori remains unknown. in this study, we found that oxidative stress played a critical role in the augmented mucosal damage provoked by water immersion restraint stress (wirs) in h. pylori infection and that an antioxidant, alpha-tocopherol, could ameliorate the aggravat ... | 2005 | 15890619 |
| helicobacter pylori and other helicobacter species in gallbladder and liver of patients with chronic cholecystitis detected by immunological and molecular methods. | despite several recent reports on the detection of helicobacter dna in human bile, there are still uncertainties concerning the correlation of these findings with biliary tract and liver diseases. | 2005 | 15841721 |
| primerselect: a transcriptome-wide oligonucleotide primer pair design program for kinetic rt-pcr-based transcript profiling. | we describe primerselect, a program capable of transcriptome-wide design of primer pairs for optimal performance in kinetic reverse-transcriptase polymerase chain reaction (rt-pcr). for the yeast saccharomyces cerevisiae, primerselect designs primer pairs for 86% of genomic open reading frames (orfs) using design criteria we previously established to be optimal for kinetic rt-pcr (krt-pcr)-based transcript quantitation. primer pairs designed by primerselect for 230 yeast orfs were evaluated for ... | 2005 | 15865983 |
| alpha-catenin expression is decreased in patients with gastric carcinoma. | to assess the expression of alpha-catenin in gastric carcinoma and to determine the role of alpha-catenin expression in gastric carcinogenesis. | 2005 | 15948257 |