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ability of bacteria associated with chronic inflammatory disease to stimulate e-selectin expression and promote neutrophil adhesion.porphyromonas gingivalis, pseudomonas aeruginosa, and helicobacter pylori have been shown to be associated with adult periodontal disease, chronic lung infections, and peptic ulcers, respectively. the ability of these bacteria to stimulate e-selectin expression and promote neutrophil adhesion, two components necessary for the recruitment of leukocytes in response to infection, was investigated. little or no stimulation of e-selectin expression was observed with either p. gingivalis or h. pylori ...19957534275
royal society of tropical medicine and hygiene meeting at manson house, london, 16 february 1995. aspects of helicobacter pylori infection in the developing and developed world. helicobacter pylori infection, nutrition and growth of west african infants.helicobacter pylori is probably the commonest bacterial infection of humankind. in adults, colonization of the stomach is associated with chronic gastritis and duodenal ulcer disease. however, children in the developing world acquire h. pylori soon after birth, and there is evidence that it plays a part, through suppression of the gastric acid barrier, in the pathogenesis of the syndrome of diarrhoea, malnutrition and growth failure. infants born of mothers who secrete milk with high levels of a ...19957570858
expression of adhesion molecules on human granulocytes after stimulation with helicobacter pylori membrane proteins: comparison with membrane proteins from other bacteria.type b gastritis in its active form is characterized by a dense infiltration of the lamina propria with granulocytes. since the bacterium helicobacter pylori does not invade the epithelial barrier, a signaling pathway chemoattractive for granulocytes must exist across this mucosal boarder. one possible mechanism tested was whether granulocytes are directly activated by water-soluble membrane proteins (wsp) from h. pylori. these findings were compared with the effects of wsp from other bacteria ( ...19957540595
production of acute gastric erosions in the cat by individual human pepsins.1. differing amounts of human pepsins 1, 3 (pepsin a) and 5 (pepsin c or gastriscin) in aqueous hcl/nacl at ph 1.3 were placed in perspex rings on the exposed luminal surface of the perfused cat stomach to test for mucolytic and erosive activity in vivo, with the acid medium in control rings. 2. after incubation at 37 degrees c for 60 or 120 min, the test and control solutions were replaced by the same volume of each test or control solution containing 18 mg of aspirin and incubated for a furthe ...19957705000
adhesion to and invasion of hela cells by helicobacter pylori.eight clinical isolates and two reference strains of helicobacter pylori were studied with regard to their interactions with hela cells. all the isolates adhered poorly to hela cells and the number of invasive bacteria was very low. no correlation was found between the adherence and invasiveness of the isolates on one hand, and the corresponding patients having ulcer or non-ulcer disease, or the ability of the strains to produce cytotoxin and to induce an oxidative burst of human polymorphonucle ...19957734829
treatment of helicobacter pylori infection.helicobacter pylori infects the human stomach and is associated with chronic gastritis, peptic ulcer disease and gastric cancer. most patients with peptic ulcers are infected with h pylori. these patients suffer a high relapse rate following initial healing of the ulcer with anti-secretory agents. the rate of relapse can be greatly diminished following successful eradication of the organism. although experience is still being accumulated, it is reasonable to use eradication therapy in any patien ...19957749079
a 35-year-old man with epigastric pain. 19957629961
[helicobacter pylori infection in children].helicobacter pylori (hp) is a pathogen of human gastric mucosa and is considered as the major cause of chronic gastritis and duodenal ulcer disease recurrence. the prevalence rate of hp infection increases with age and is related to low socioeconomic status in childhood. childhood appears to be a high-risk period for hp infection. clinical and histological features of hp infection are variable and often moderate in children. this is probably due to both genetic and environmental factors. abdomin ...19957640761
comparison of helicobacter mustelae and helicobacter pylori adhesion to eukaryotic cells in vitro.bacterial adhesion to mucosal surfaces is an important pathogenic mechanism for helicobacter-induced gastritis. the aims of this study were to compare binding of selected helicobacter mustelae and helicobacter pylori strains to lipids extracted from hep-2, chinese hamster ovary, human embryonic lung cells, and ferret gastrointestinal tissues as well as to intact tissue culture cells and to analyze the fatty acids of the receptor.19957657097
early gastric cancer in a patient with menetrier's disease, lymphocytic gastritis and helicobacter pylori.to describe a patient with early gastric cancer, menetrier's disease, lymphocytic gastritis and helicobacter pylori infection.19957712313
helicobacter pylori: human pathogen or simply an opportunist? 19957715345
activation of human thp-1 cells and rat bone marrow-derived macrophages by helicobacter pylori lipopolysaccharide.the mechanism by which helicobacter pylori, which has little or no invasive activity, induces gastric-tissue inflammation and injury has not been well characterized. we have previously demonstrated that water-extracted proteins of h. pylori are capable of activating human monocytes by a lipopolysaccharide (lps)-independent mechanism. we have now compared activation of macrophages by purified lps from h. pylori and from escherichia coli. lps was prepared by phenol-water extraction from h. pylori ...19957890370
characterization of a helicobacter pylori neutrophil-activating protein.helicobacter pylori-associated gastritis is mainly an inflammatory cell response. in earlier work we showed that activation of human neutrophils by a cell-free water extract of h. pylori is characterized by increased expression of neutrophil cd11b/cd18 and increased adhesiveness to endothelial cells. the work reported here indicates that the neutrophil-activating factor is a 150,000-molecular-weight protein (150k protein). neutrophil proadhesive activity copurified with this protein, which is a ...19957768601
uptake site of lansoprazole, a proton pump inhibitor, in human fundic mucosa: possible relevance with fibroblast and helicobacter pylori.to clarify the mechanism of the effect of lansoprazole in the healing of human gastric ulcer, the uptake sites of lansoprazole were studied using endoscopically biopsied specimens from the margin of the gastric ulcer. the specimens were incubated in a medium containing 3h-lansoprazole for 5 or 15 min., postfixed with 1% osmic acid and embedded in epon. the semithin or ultrathin sections were made and radioautographic emulsion films were applied by the wire-loop method. 30 days after the incubati ...19957773126
protein hpn: cloning and characterization of a histidine-rich metal-binding polypeptide in helicobacter pylori and helicobacter mustelae.helicobacter pylori is a human gastrointestinal pathogen involved in gastritis, duodenal ulcers, and gastric neoplasia. this microorganism produces large amounts of a urease which, like all known ureases, has nickel in the active site. we have identified a protein in clinical isolates of h. pylori and an identical protein in the ferret pathogen helicobacter mustelae that strongly binds ni2+ and zn2+. this protein has been named hpn to emphasize its origins in h. pylori and its affinity for nicke ...19957790085
human milk kappa-casein and inhibition of helicobacter pylori adhesion to human gastric mucosa.readily digested caseins, which account for almost half of the protein content in human milk, are important as nutritional protein for breast-fed infants. it has also been advocated that part of the antimicrobial activity of human milk resides in the caseins, most likely the glycosyated k-casein. top explore this possibility, we purified k-casein from human milk to homogeneity by a two-step size-exclusion chromatography procedure. purified human k-casein, in contrast to k-casein purified from bo ...19958523212
immunogenetic analysis of the human leukocyte antigen dqa1 locus in patients with duodenal ulcer or chronic atrophic gastritis harbouring helicobacter pylori.to examine the human leukocyte antigen (hla)-dqa1 locus in patients harbouring helicobacter pylori with chronic atrophic gastritis or duodenal ulcer as part of an investigation into immunogenetic differences in the host.19958574742
effect of il-4 on peripheral blood lymphocyte proliferation: implication in immunopathogenesis of h. pylori infection.interleukin (il) 4 is a type 2 cytokine which has a negative immunoregulatory role in human infection. il-4 suppresses the production of interferon-gamma and enhances il-10 synthesis. however, the effect of il-4 on proliferative response of lymphocytes remains to be elucidated. we have previously reported an increase in production of il-4 in subjects with helicobacter pylori (h. pylori) infection. to evaluate whether the increased il-4 is responsible for the down-regulation of immune responses i ...19958847090
reduced amoxicillin uptake into human gastric mucosa when gastric juice ph is high.amoxicillin when administered with gastric acid suppressors has been shown to be effective in eradication of helicobacter pylori in 50 to 80% of subjects. the aim of this investigator-blind crossover study was to determine if gastric mucosal amoxicillin uptake was affected by increasing gastric juice ph. fifteen male subjects (7 h. pylori positive and 8 h. pylori negative) were randomized to receive 150 mg of ranitidine twice a day, 300 mg of ranitidine twice a day, or no drug for 2 days prior t ...19958540720
intrastrain differences in helicobacter pylori: a key question in mucosal damage?helicobacter pylori causes persistent infection and inflammation in the human stomach, yet only a small fraction of infected people develop illness. an important question is why this diversity exists in infection outcome. in recent years, there has been evidence of substantial phenotypic as well as genotypic diversity of h. pylori. three different phenotypes--production of vacuolating cytotoxin, presence of caga, and ability for strong pmn activation--appear to be linked to one another and to th ...19958541032
spiral bacteria in the human stomach: the gastric helicobacters.during the past decade, helicobacter pylori has become recognized as one of the most common human pathogens, colonizing the gastric mucosa of almost all persons exposed to poor hygienic conditions from childhood. it also is often found, albeit with a lower frequency, in groups of high socioeconomic status. h. pylori causes chronic active gastritis and is a major factor in the pathogenesis of duodenal ulcers and, to a lesser extent, gastric ulcers. in addition, the presence of this bacterium is n ...19958903168
early ultrastructural changes of antral mucosa with aspirin in the absence of helicobacter pylori.to describe the ultrastructural changes that occur in human antral mucosa following direct application of aspirin in volunteers without helicobacter pylori infection.19958543644
[helicobacter pylori and azithromycin].helicobacter pylori is a gram-negative bacterium with man as the unique reservoir and where the niche is the stomach. transmission between individuals could be by fecal and oral route. in the stomach, the bacterium is mainly located at the mucus level and adhere to antral cells which are the privileged target. within the colonized mucosa, h. pylori generates or secretes different deleterious compounds against the epithelial cells: urease, hydrolyzing urea into ammonia, a cytotoxic agent: monochl ...19958539082
the significance of helicobacter pylori colonization of the stomach.helicobacter pylori (hp) was discovered in 1982 by the australians robin warren and barry marshall. initially rejected by a skeptical scientific community, it has since gained worldwide recognition as a clinically significant bacterium. the incidence of colonization with hp increases with age, affecting approximately one third of the world's population. hp is uniquely capable of surviving in the acid environment of the stomach, and has properties of adherence to epithelial cells that resist para ...19958548210
bacteriology of helicobacter pylori.the discovery and first isolation of h. pylori in pure culture from gastric biopsies in 1982 provided the basis for a completely new area of microbiology. since then, h. pylori has been an intensively pursued topic world-wide, and extensive data have been acquired on all aspects of its basic microbiology, both at the conventional phenotypic level and at the molecular level. h. pylori is a remarkable microorganism because of its ability to readily colonize a major proportion of human population w ...19958563046
animal models and vaccine development.following the demonstration of helicobacter pylori as a major gastroduodenal pathogen there was a need to develop animal models in order to investigate mechanisms of pathogenesis and to be able to test new treatment strategies. helicobacter pylori will only colonize a limited number of hosts including non-human primates, germ-free or barrier raised piglets, germ-free dogs and recently laboratory raised cats. although these models have proved useful there is a need for more convenient small anima ...19958563056
investigation of the metabolism of ebrotidine in human urine by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry.ebrotidine is a new h2-receptor antagonist that, in addition to its antisecretory activity, exhibits a remarkable ability for gastric mucosal protection and acts as a potent inhibitor of protease and lipase enzymes elaborated by helicobacter pylori. to study the metabolism of ebrotidine in human urine, hplc/ms with an atmospheric pressure chemical ionization (apci) interface and simultaneous uv detection was conducted. hplc/ms separation of the reference compounds was performed, and positive and ...19958565788
localization of sulfatides in the epithelial lining of gastric mucosa: studies with a monoclonal antibody to sulfatides.a specific monoclonal antibody against sulfatides was used to examine the cellular localization of sulfatides, the most predominant acidic glycosphingolipids in both rabbit and human gastric mucosa. the monoclonal antibody selectively recognized sulfatides among the acidic glycolipids extracted from rabbit gastric mucosa. immunofluorescence staining revealed that specific staining was localized in the epithelial cells of the stomach. both epithelial and glandular cells were stained in the fundic ...19958774999
gastric inflammation and neutrophil-activating and cytotoxin-producing helicobacter pylori strains.some helicobacter pylori strains activate human neutrophils without opsonins and/or produce vacuolating cytotoxin.19968819210
the stimulation and inhibition of t cell proliferation by helicobacter pylori components.the purified t cells from peripheral blood of healthy human volunteers, seronegative for anti-helicobacter pylori antibody were stimulated in cultures with live or heat-killed h. pylori rods or with bacterial sialic acid-specific surface haemagglutinin (sha), a crude surface (sf) or cytoplasmic (cf) fractions. it is demonstrated that h. pylori bacteria contain both stimulatory and inhibitory components for t cells of healthy individuals. the sha as well as sf (5-20 micrograms) induced the prolif ...19968777299
[the milk-alkali syndrome--a rare differential diagnosis for hypercalcemia].a 54-year-old man was hospitalised because hypercalcaemia and associated renal failure were suspected. he had a history suggesting gastric ulcer, with nonspecific back and shoulder pain and spontaneously passed kidney stone. on admission the patient reported reduction in physical fitness, fatigue, headache and nausea without vomiting. physical examination was unremarkable except for pain on pressure over the thoracic and lumbar vertebrae.19968801073
manifestations of the local gastric immune response in gnotobiotic piglets infected with helicobacter pylori.helicobacter pylori, a human gastric bacterial pathogen, was inoculated into gnotobiotic piglets and manifestations of the resultant gastric inflammation was analyzed by in situ immunochemistry and flow cytometric analysis of isolated lamina propria leukocytes (lpl) and peripheral blood leukocytes (pbl) recovered from infected and control piglets. gastric mucosa tissue sections from uninfected control piglets were essentially negative for cluster differentiation- (cd-) positive leukocytes. failu ...19968809998
resolution of a metaplastic duodenal polyp after cure of helicobacter pylori infection.although there have been several reports of hyperplastic gastric polyps associated with persistent helicobacter pylori gastritis, the association of h. pylori infection with metaplastic polyps in the duodenum has not hitherto been described. after a 52-year-old man had a single episode of hematemesis, endoscopy showed a smooth polyp 1 cm in greatest dimension formed by a proliferation of gastric epithelial cells of fundic and antral type found in the duodenal bulb. the outer surface was entirely ...19968835902
the safety and efficacy of ranitidine bismuth citrate in combination with antibiotics for the eradication of helicobacter pylori.ranitidine bismuth citrate is a novel salt of ranitidine and a bismuth citrate complex. it has intrinsic antisecretory and anti-helicobacter pylori activity, but monotherapy rarely eradicates h. pylori infection in man.19968853768
antigastric autoantibodies in helicobacter pylori gastritis: prevalence, in-situ binding sites and clues for clinical relevance.colonization of human gastric mucosa with helicobacter pylori leads to chronic active gastritis and induces the occurrence of an acquired mucosa-associated lymphoid tissue (malt) in the stomach. this remodelling of the gastric mucosa together with chronic antigen persistence may induce autoimmune reactions. the aim of this study was to investigate humoral autoimmune reactions to human gastric mucosa in h. pylori gastritis and their clinical relevance. sera from patients with dyspeptic symptoms w ...19968624577
helicobacter pylori urease is a potent stimulus of mononuclear phagocyte activation and inflammatory cytokine production.helicobacter pylori surface proteins induce the production of proinflammatory mediators by mononuclear phagocytes, but the protein responsible for this stimulation has not been identified. this study determined whether urease, the major component of the soluble proteins extracted from h. pylori grown in culture, activates mononuclear phagocytes and stimulates them to produce proinflammatory cytokines.19968690207
use of pcr and culture to detect helicobacter pylori in naturally infected cats following triple antimicrobial therapy.helicobacter pylori causes gastritis and peptic ulcers and is linked to gastric cancer. domestic cats from a commercial source were found to be naturally infected with h. pylori, and studies were undertaken to eradicate h. pylori from infected cats by using triple antimicrobial therapy. eight cats infected with h. pylori were used in the study. six cats received a 21-day course of oral amoxicillin, metronidazole, and omeprazole, and two cats served as controls. two weeks and 4 weeks posttreatmen ...19968726024
biochemical aspects of helicobacter pylori colonization of the human gastric mucosa.unlike helicobacter felis and other helicobacter species of animal origin, helicobacter pylori colonizes the lower gastric mucin layer of the stomach and adheres to human gastric epithelial cells. it is still an open question if h. pylori can interact with specific glycoconjugates in the gastric mucin layer. it is possible that colonization of the oral cavity is a first step of a complex infectious process. most likely resting or slow growing cells of h. pylori interact with lewis blood group su ...19968730256
helicobacter pylori infection and its role in human disease--an overview.helicobacter pylori (h. pylori) is probably the commonest chronic bacterial infection in humans. the bacterium colonizes the gastric epithelium and induces inflammation of the underlying mucosa. the recognition that this infection is the most important acquired factor in the pathogenesis of duodenal and gastric ulcers has transformed the management of these common disorders. most patients with ulcers can be cured by a 1-week course of anti-h. pylori therapy, thereby removing the need for long-te ...19968739257
specific neutrophil hyporesponsiveness in chronic helicobacter pylori infection.the release from mucosal neutrophils of reactive oxygen species (ros) is believed to be an important part of the pathogenesis of helicobacter pylori-associated gastritis and duodenal ulcer. in this study, bacterial sonicates prepared from 1 reference and 29 patient strains were used to stimulate ros release from peripheral blood neutrophils. in 29 h. pylori-positive patients, the neutrophil response toward the homologous strain was absent. in contrast, a significant response was observed toward ...19968769612
[effect of homogenized human pathogenic helicobacter pylori on the viability of isolated rat gastric mucosal cells].aim of this study was to detect the possible direct cellular effect of helicobacter pylori (hp) (obtained by biopsy from patients with gastric and duodenal ulcer) on freshly isolated rat gastric mucosal cells. urease test positive hp cultured from human biopsy sample was sonicated and incubated with freshly isolated rat gastric mucosal cells. the direct cellular damaging effect of hp was evaluated alone and in combination with ethanol and indomethacin. the cellular damaging effect was tested mor ...19968966016
gastric mucosal biopsy via a nasogastric tube: a nonendoscopic method for diagnosing fundic and antral mucosal gastritis and helicobacter pylori infection in man. 19968885353
[gastrin and gastrin receptor].gastrin is a well known endogenous stimulator of gastric acid. in addition, recent studies have revealed that gastrin has a growth promoting effect on gastric ecl cells. indeed, development of ecl carcinoid tumor occurs almost exclusively in patients with hypergastrinemia such as autoimmune gastritis and zollinger-ellison syndrome with men type i. we have recently cloned human gastrin receptor gene, and by using it, we found that both gastric carcinoid tumor and endocrine cell carcinoma of the s ...19968920678
the vacuolar atpase proton pump is present on intracellular vacuoles induced by helicobacter pylori.cytotoxic strains of helicobacter pylori cause an intense vacuolar degeneration of cells, due to the enlargement of late endosomes in the presence of membrane permeant weak bases. bafilomycins, specific inhibitors of the vacuolar-type (v-) atpase proton pump, prevent vacuole formation. the presence of the v-atpase on vacuolar membranes was demonstrated by immunofluorescence with a monoclonal antibody (mab) specific for the human 116-kda regulatory subunit. the v-atpase co-localised with the late ...19968683556
extract of helicobacter pylori induces neutrophils to injure endothelial cells and contains antielastase activity.previous studies indicate that a water extract of helicobacter pylori promotes leukocyte adhesion and emigration as well as endothelial barrier disruption (increased vascular protein leakage) in rat mesenteric venules. the aims of this study were to assess whether h. pylori extract-activated neutrophils disrupt endothelial cell monolayers and to identify the mechanisms involved in this process.19968536858
the novel selected-ion flow tube approach to trace gas analysis of air and breath.we present an overview of the development and use of our selected-ion flow tube (sift) technique as a sensitive, quantitative method for the rapid, real-time analysis of the trace gas content of atmospheric air and human breath, presenting some pilot data from various research areas in which this method will find valuable application. we show that it is capable of detecting and quantifying trace gases, in complex mixtures such as breath, which are present at partial pressures down to about 10 pa ...19968759327
cell surface glycosaminoglycans are not involved in the adherence of helicobacter pylori to cultured hs 198.st human gastric cells, hs 746t human gastric adenocarcinoma cells, or hela cells.hs 198.st cells (a line derived from normal human gastric tissue), hs 746t cells (a line derived from human gastric adenocarcinoma), and hela cells were used together with 3h-labelled helicobacter pylori, strain nctc 11637 to determine if cell surface glycosaminoglycans could act as initial receptors for adherence of the bacteria. although as much as 40% of the 3h-labelled bacteria adhered to monolayers of the cultured cells, removal of glycosaminoglycans by prior treatment of the cells with hep ...19968910015
binding and internalization of the helicobacter pylori vacuolating cytotoxin by epithelial cells.many helicobacter pylori strains produce a cytotoxin (vaca) that induces vacuolation in epithelial cells. in this study, binding and internalization of the cytotoxin by hela or ags (human gastric adenocarcinoma) cells were characterized by indirect fluorescence microscopy. cells incubated with the cytotoxin at 4 degrees c displayed a uniform fluorescent plasma membrane signal. preincubation of the cytotoxin with either rabbit antiserum to approximately 90-kda h. pylori vaca or sera from h. pylor ...19968926088
effects of sucralfate on vacuolating cytotoxin activity and adherence of helicobacter pylori to human gastric epithelial cells.sucralfate inhibits activity of certain helicobacter pylori enzymes, implying that this medication may limit gastric cell injury associated with h pylori infection. this study evaluates the ability of sucralfate and its two major structural components, sucrose octasulfate and aluminum hydroxide, to reduce the cytotoxic effects of h pylori and to inhibit binding of h pylori to human gastric epithelial cells. experiments were performed using human gastric epithelial cells isolated from gastric bio ...19968936934
effects of omeprazole and amoxycillin on the human oral and gastrointestinal microflora in patients with helicobacter pylori infection.fourteen patients with helicobacter pylori infection were treated with omeprazole capsules 20 mg and amoxycillin capsules 1000 mg twice daily for 14 days and 14 patients with omeprazole capsules 20 mg and placebo twice daily for 14 days. samples from saliva, dental plaque and faeces and biopsies from antrum and corpus were analysed in order to determine the ecological changes in the normal microflora. several microorganisms were affected by both treatment regimens. two patients were colonised wi ...19969023640
simple and rapid quantitative assay of 13c-labelled urea in human serum using liquid chromatography-atmospheric pressure chemical ionization mass spectrometry.a simple and rapid quantitative method for 13c-labelled urea ([13c]urea) in human serum was developed by using high-performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (hplc-apci-ms). this method is used to establish and normalize the [13c]urea breath test, which is considered as an effective diagnostic method for helicobacter pylori infection. hplc-apci-ms, involving a simple pretreatment process such as diluting serum with water, was shown to be able to ...19968891910
role of vaca and the caga locus of helicobacter pylori in human disease.helicobacter pylori are 'slow' bacteria that may cause disease decades after acquisition. bacterial pathogenesis often involves features, including conserved genes, shared by many different species. as such, despite its unique niche in the human body, the pathogenesis of h. pylori infection most likely shares mechanisms with other bacteria. in this paper, two genes, vaca and caga, which appear unique to h. pylori and which may reflect the particular requirements of h. pylori for long-term reside ...19968730262
helicobacter colonization of biopsy specimens cultured in vitro is dependent on both mucosal type and bacterial strain.colonization by helicobacter pylori is strictly tissue-specific. we have previously reported on an in vitro adhesion model for pig and human gastric mucosa, in which biopsy specimens were successfully infected and cultured for 72h. the aim of this study was to compare h. pylori colonization of different mucosae and by different helicobacter strains.19968734338
effects of misoprostol on healing and prevention of biopsy-induced gastroduodenal lesions occurring during the administration of diclofenac to volunteers.to determine whether misoprostol promotes the healing of non-steroidal anti-inflammatory drug-induced gastroduodenal lesions in a human experimental model.19968853760
helicobacter pylori eradication: therapy other than with bismuth or proton-pump inhibitors.antibiotics alone do not achieve helicobacter (h. pylori) eradication perhaps because of reduced activity in an acid environment. some reports suggest that ranitidine combined with amoxicillin and metronidazole is highly successful in eradicating h. pylori, but other have found less success with h2-antagonists. studies have shown that sucralfate is equally as effective as omeprazole when combined with clarithromycin and metronidazole in achieving h. pylori eradication. similarly, sucralfate trip ...19968898434
molecular mimicry of host structures by bacterial lipopolysaccharides and its contribution to disease.the core oligosaccharides of low-molecular-weight lipopolysaccharide (lps), also termed lipooligosaccharide (los), of pathogenic neisseria spp. mimic the carbohydrate moieties of glycosphingolipids present on human cells. such mimicry may serve to camouflage the bacterial surface from the host. the los component is antigenically and/or chemically identical to lactoneoseries glycosphingolipids and can become sialylated in neisseria gonorrhoeae when the bacterium is grown in the presence of cytidi ...19968988391
gastric malt lymphoma and helicobacter pylori.primary gastric low-grade b-cell lymphomas are neoplastic mimics of mucosa associated lymphoid tissue (malt) as exemplified by peyer's patches in the terminal ileum. architectural and immunophenotypic properties of the neoplastic cells suggest that they originate from malt-derived marginal zone b-cells. paradoxically, the normal human stomach is devoid of organized malt within which a lymphoma can develop. lymphoid tissue is acquired in the stomach in response to antigenic stimulation, predomina ...19969041690
helicobacter pylori and the surface mucous gel layer of the human stomach.the colonization of helicobacter pylori in the surface mucous gel layer (smgl) was investigated.19969398871
presentation and management of helicobacter pylori infection in childhood.helicobacter pylori is responsible for one of the most frequently encountered infectious diseases worldwide. helicobacter pylori infection can lead to the development of gastritis and peptic ulcer disease. the presence of helicobacter pylori in the human stomach also represents an increased risk of gastric cancer and gastric lymphoma. epidemiological data obtained in adults suggest that the actual colonization with helicobacter pylori is in fact determined by childhood factors. therefore, the pe ...199610830008
helicobacter pylori in tumor tissues of patients with advanced gastric adenocarcinoma: high prevalence but failure to detect integration.helicobacter pylori has been known to be associated with gastric adenocarcinoma by case control studies. however, significant portion of patients with gastric carcinoma are negative for h. pylori by serological test. to further detect the presence of h. pylori infection in serum and tissue of patients with gastric adenocarcinoma, paired tissues and serum samples from 32 patients with gastric adenocarcinoma were tested. antibodies to h. pylori were tested by an enzyme linked immunosorbent assay ( ...199610592795
helicobacter pylori infection and tumour necrosis factor-alpha increase gastrin release from human gastric antral fragments.to investigate the mechanism of the hypergastrinaemia associated with helicobacter pylori infection by examining the effect of h. pylori infection and the cytokine tumour necrosis factor-alpha (tnf-alpha) on gastrin release from human antral fragments.19979282274
antibacterial activity of (+/-) 6-benzyl-1-(3-carboxypropyl) indane; a possible way to identify leading novel anti-h. pylori agents.magainin 2, isolated from the skin of the xenopus laevis, is an antimicrobial peptide which reacts directly with the biological membrane to lyse various bacteria from negative and positive microorganisms. in a previous report, we showed that (+/-)1-(4-aminobutyl)-6-benzylindane (pm2), which mimicked the conformation of the side-chains of a complementary unit on the amino acid sequence of magainin 2 analogs, expressed the in vitro antibacterial activity not only against helicobacter, pylori (atcc ...19979401733
are helicobacter pylori differences important in the development of helicobacter pylori-related diseases?helicobacter pylori colonises the stomach of man and induces a strong mucosal inflammation and a local and systemic immune response. differences in virulence characteristics of helicobacter pylori isolates can account for different clinical outcomes of infection. most determinants of helicobacter pylori pathogenicity factors are present in all isolates examined; some are present only in or expressed more intensively by certain strains. many enzymes, i.e., urease, mucinase, phospholipases, alcoho ...19979476194
mucosal chemokine activity in helicobacter pylori infection.we examined secretion, mrna expression, and histologic localization of interleukin-8 (il-*) and growth-related gene product-alpha (gro alpha) in the helicobacter pylori-infected gastric antral mucosa. antral biopsies were obtained from an area of endoscopically intact mucosa. significantly higher levels of il-8 and gro alpha were secreted in organ cultures from patients with h. pylori infection, and their elevation was prominent in patients with duodenal ulcer. there was a significant associatio ...19979479649
mucosa-associated lymphoid tissue lymphoma coexisting with epithelioid granulomas in the stomach of a patient with systemic sarcoidosis.malignant lymphoma arising in the stomach of a 23-year-old japanese man with systemic sarcoidosis is presented. the patient was followed because of systemic sarcoidosis involving the lungs, eyes, and lymph nodes. biopsy specimens from the stomach were repeated because of recurrent epigastralgia and multiple ulcerations. some of the specimens revealed epithelioid granulomas with no caseous necrosis, which confirmed gastric involvement of sarcoidosis. three years after the initial diagnosis, biops ...19979503470
helicobacter pylori, individual host specificity and human disease. european helicobacter study group meeting, copenhagen, october 16-19, 1996. 19979008421
heat-shock protein 60 homologue of helicobacter pylori is associated with adhesion of h. pylori to human gastric epithelial cells.a previous study reported a relationship between the expression of heat-shock protein 60 (hsp60) by helicobacter pylori and its adhesion to human gastric carcinoma (mkn45) cells. to examine whether the hsp60 homologue of h. pylori is associated with the adhesion of h. pylori to human gastric epithelial cells, an inhibition assay of adhesion of h. pylori to mkn45 cells was performed by flow cytometric analysis with monoclonal antibody (mab) designated as h20 recognising hsp60 of h. pylori. the ra ...19979364138
recognition of glycoconjugates by helicobacter pylori. comparison of two sialic acid-dependent specificities based on haemagglutination and binding to human erythrocyte glycoconjugates.helicobacter pylori expresses separate binding characteristics depending on growth conditions, as documented by binding to human erythrocyte glycoconjugates. cells grown in ham's f12 liquid medium exhibited a selective sialic acid-dependent binding to polyglycosylceramides, pgcs (miller-podraza et al.(1996) glycoconjugate j13:453-60). there was no binding to traditional sialylated glycoconjugates like shorter-chain gangliosides, glycophorin or fetuin. however, cells grown on brucella agar bound ...19979249144
attachment of helicobacter pylori strains to human epithelial cells.the aim of the study was to characterize several clinical isolates of h. pylori as regards the activity and specificity of their haemagglutinins and the involvement of surface sialic acid-specific and heparin-binding compounds in the adhesin of the bacteria to human epithelial cell lines. although h. pylori strains caused haemagglutination (ha) of sheep erythrocytes, they differed markedly by activity and specificity. on the basis of haemagglutination inhibition study three types of h. pylori st ...19979376622
helicobacter pylori survival in gastric mucosa by generation of a ph gradient.helicobacter pylori has been established as the major causative agent of human active gastritis and is an essential factor in peptic ulcer disease and gastric cancer. the mechanism that has been proposed for h. pylori to control its inhospitable microenvironment happens to coincide with the ph control technique developed by us. this technique was developed to separate an acidic environment from a basic environment for a sequential enzymatic reaction by the hydrolysis of urea within a thin layer ...19979251824
ecology of helicobacter pylori in the human stomach. 19979259572
molecular mechanisms for inflammation-promoted pathogenesis of cancer--the sixteenth international symposium of the sapporo cancer seminar. 19979270037
how is helicobacter pylori transmitted?helicobacter pylori is one of the world's most common pathogens. it colonizes about 60% of the world's population, causes gastritis and peptic ulcer, and is strongly associated with gastric adenocarcinoma and lymphoma. however, most individuals never develop clinical disease. thirteen years after the culture of h. pylori by marshall and warren, we still do not know its major mode of transmission. childhood represents the major period of acquisition of infection in the third world, but infection ...19979394753
evaluation of a rapid, new method for detecting serum igg antibodies to helicobacter pylori.there is an increased need for rapid, inexpensive tests to diagnose helicobacter pylori infection. our objective was to determine the performance characteristics of an immunochromatographic test (ict) for detection of anti-h. pylori igg antibodies. a commercially available ict kit (flexsure hp) was tested with a well-characterized cohort of banked sera as well as with fresh serum from randomly selected symptomatic patients. the ict was evaluated with 107 stored sera and 96 prospective patients. ...19979166238
binding of helicobacter pylori to sialic acid-containing glycolipids of various origins separated on thin-layer chromatograms.two standard strains of helicobacter pylori, grown on solid or in liquid medium, were studied for their binding to sialic acid-containing glycosphingolipids on thin-layer plates. nctc 11637, but not strain 11638, bound to mixtures of gangliosides of various human and animal origins with similar binding patterns and also to polyglycosylceramides of human erythrocytes, leukocytes, and placenta. there was an apparent specificity for neuac alpha3gal of the neolacto series of gangliosides, since neua ...19979169796
helicobacter pylori stimulates granulocyte-macrophage colony-stimulating factor (gm-csf) production from cultured antral biopsies and a human gastric epithelial cell line.to examine the regulation of granulocyte-macrophage colony-stimulating factor (gm-csf) production by gastric epithelial cells in helicobacter pylori infection.19979187876
role of lipopolysaccharide and related cytokines in helicobacter pylori infection.helicobacter pylori is a gram-negative bacterium which accounts for the development of chronic gastritis and peptic ulcer in man. in this review, emphasis has been laid on the role of lipopolysaccharide (lps) of the h. pylori cellular wall in the pathogenesis of gastroduodenal disease. h. pylori lps exhibits a reduced endotoxic potency in terms of pyrogenicity, lethality, toxicity, mitogenicity, cytokine (ck) release and chromogenic limulus amebocyte lysate assay. this low biological activity of ...19979200967
carbohydrate binding specificity of the neutrophil-activating protein of helicobacter pylori.the possible interaction of the neutrophil-activating protein of helicobacter pylori with target cell glycoconjugates was investigated by the binding of 125i-labeled recombinant protein to glycosphingolipids from human neutrophils in solid phase assays. thereby, a distinct binding of the neutrophil-activating protein to four bands in the acid glycosphingolipid fraction from human neutrophils was detected, whereas no binding to the non-acid glycosphingolipids or polyglycosyl ceramides from these ...19979228091
increased motility of helicobacter pylori by methylcellulose could upregulate the expression of proinflammatory cytokines in human gastric epithelial cells.the inflammatory reaction in the human gastric mucosa to helicobacter pylori could be initially triggered by an array of cytokines expressed in infected gastric epithelial cells. the spiral morphology and flagella of these organisms could increase their velocity in a viscous environment such as methylcellulose solution. the goal of this study was to determine whether modification of h. pylori motility could influence the expression of cytokine genes from gastric epithelial cells infected with h. ...19979238762
a rapid and simple method to quantify helicobacter pylori adhesion to human gastric mkn-28 cells.a rapid and simple method to quantitatively analyse helicobacter pylori adhesion to human gastric epithelial cells was developed by using an enzyme-linked immunosorbent assay. this method made it possible to quantitatively evaluate the adhesion activities of many different h. pylori strains at a time. our studies indicate that this method is well suited for the quantitative analysis of h. pylori adhesion to gastric epithelial cells.19979195383
viewing aids from a glycobiological perspective: potential linkages to the human fetoembryonic defence system hypothesis.the primary molecular changes that lead to development of acquired immunodeficiency syndrome (aids) are very poorly understood, as are the mechanisms underlying the protection of the developing human from the maternal immune response. recent data that the human immunodeficiency virus (hiv) may be using the glycosylation system of the t lymphocytes to acquire glycans for its glycoproteins that enable it to disrupt carbohydrate dependent immune cell interactions or induce aberrant immune reactions ...19979239703
seroepidemiological study of helicobacter pylori infection in south african children.the seroepidemiology of helicobacter pylori infection was studied in 681 randomly selected black children from newborn to 13 years of age (333 boys, mean age 8.05 years, and 348 girls, mean age 7.76 years) in kwazulu/natal, south africa. h. pylori infection was identified serologically using an enzyme-linked immunosorbent assay to detect the presence of immunoglobulin g against h. pylori. demographic information collected included age, gender, family income, overcrowding, educational level, and ...19979373630
[the modes of transmission of helicobacter pylori infection].helicobacter pylori plays an essential role in the development of several both acid-related and neoplastic gastroduodenal pathologies. there are still uncertainties about the transmission routes and the sources of h. pylori infection. man is the only well established "reservoir" of h. pylori, while the role of other mammalians (cat, pig, primates), as sources of infection, is still controversy. literature data suggest four different modalities of transmission of the infection: faeco-oral, oro-or ...19979244959
monoclonal antibodies against helicobacter pylori cross-react with human tissue.h. pylori is a causative agent of chronic gastritis. however, the pathogenic mechanism by which h. pylori induces chronic inflammation and epithelial injuries in the gastric and duodenal mucosa is not well known. investigators have recently reported that some monoclonal antibodies against h. pylori cross-react with the gastric epithelial cells. so, there exists the possibility that the autoimmune mechanism may be involved in the pathogenesis of chronic gastritis caused by h. pylori. the purpose ...19979421126
seroepidemiology of helicobacter pylori infection and hepatitis a in a rural area: evidence against a common mode of transmission.recent studies have shown that the age-specific seroprevalence of h pylori infection parallels hepatitis a (hav), suggesting similar modes of transmission. the aim of this study was to investigate the seroepidemiology of h pylori and hav in the same setting.19979301493
in vitro evaluation of the role of antibodies against helicobacter pylori in inhibiting adherence of the organism to gastric cells.once helicobacter pylori infection is established, it is difficult to eradicate despite a persistent systemic and local immune response. it is not known whether immunisation can be used to prevent h pylori infection in humans.19979245926
prevention of helicobacter pylori infection by lactobacilli in a gnotobiotic murine model.helicobacter pylori is a bacterium which causes gastric inflammatory diseases. oral inoculation of h pylori usually results in only a temporary colonisation without a successful infection in the stomach of conventional mice in which lactobacilli are the predominant indigenous bacteria.19979274471
[flies--reservoirs and vectors of helicobacter pylori].the route by which humans get infected with h.p. is unknown. viable bacteria have been shown to be excreted in human feces from infected individuals. we have recently shown that flies are able to carry and disseminate viable h. p. with their excreta, when they have fed on h.p. we propose a hypothesis that h. p. is acquired from human feces by flies, which then, while crawling on human food, contaminate it with their intestinal excreta. the food, swallowed by a susceptible individual then leads t ...19979102386
evaluation of experimental therapeutics in a new mouse model of helicobacter felis utilizing 16s rrna polymerase chain reaction for detection.a new mouse model of helicobacter felis infection, which mimics the human infection observed with h. pylori, has recently been developed utilizing polymerase chain reaction (pcr) based on the 16s rrna gene sequence for detection of infection.19979140149
helicobacter pylori gastritis in a child with sickle cell anemia and recurrent abdominal pain.recurrent abdominal pain is a common complaint in children with sickle cell disease. helicobacter pylori gastritis has recently been described in association with recurrent abdominal pain in children.19979149750
urea breath tests for the detection of helicobacter pylori infection.helicobacter pylori is recognized as an important human pathogen. the urea breath test, using either 13c or 14c, provides a noninvasive diagnostic method for the detection of active h. pylori infection.19979432352
summary of avoidable cancers in the nordic countries.an overview is given of the most important known causes of cancer in the five nordic countries and the resulting number of cancers that are potentially avoidable. the main causes include active and passive smoking, alcohol consumption, exposure to asbestos and other occupational carcinogens, solar and ionizing radiation, obesity, human papillomavirus infection in the female genital tract and infection with helicobacter pylori. the organs most commonly affected are those of the respiratory system ...19979462826
gastric cancer and helicobacter pylori: a critical evaluation of the epidemiological evidence.despite a dramatic reduction in incidence and mortality, gastric cancer (gc) still was recently reported to be the second most common neoplastic cause of death worldwide. gc treatment has not been improved substantially, and large-scale early diagnosis programs have not proved feasible outside japan. on the other hand, even if diet has been shown to play a relevant role in the etiology of gc, primary prevention programs based on dietary modifications are difficult to implement, and results of hu ...19979432355
non-random loss of chromosome 3 during transition of helicobacter pylori-associated gastric malt to b-cell malt lymphoma revealed by fluorescence in situ hybridization.acquired gastric mucosa-associated lymphoid tissue (malt) accumulates as a result of long-standing helicobacter pylori (h. pylori) infection and from this acquired malt, low-grade b-cell malt lymphoma may develop. carcinogenesis is a multistep, multifactorial process involving the progressive accumulation of genetic changes. to determine whether numerical chromosomal alterations are involved in the transition of h. pylori-associated human gastric malt to low-grade b-cell malt lymphoma, frozen bi ...19979459178
helicobacter pylori infection activates nf-kappa b in gastric epithelial cells.helicobacter pylori adheres to gastric epithelial cells and stimulates interleukin (il)-8 production. this may be instrumental in neutrophil infiltration of the gastric epithelium that characterizes h. pylori gastritis. this study examined the molecular mechanisms leading to h. pylori-induced epithelial cell il-8 production.19979322504
hunterian peptic ulcers and helicobacter pylori.gastric spiral organisms were first described in man in 1939 and identified as helicobacter pylori causing peptic ulcers in the early 1980s. surgical specimens of gastric resections from 1939 showed h. pylori to be present. full-thickness sections of gastric mucosa from gastric specimens from the eighteenth-century hunterian collection at the royal college of surgeons of england were examined by histology for the presence of h. pylori. four gastric ulcers and a section from an oesophageal varix ...19979326130
[using polymerase chain reaction to detect helicobacter heilmannii in gastric biopsy materials].helicobacter heilmannii is one of the species of helicobacter other than helicobacter pylori noticed in human gastric mucosa. in our study, a polymerase chain reaction (pcr) was designed for the detection of helicobacter heilmannii in gastric biopsy specimens. two broad-range bacterial 16s rdna primers and one helicobacter genus-specific 16s rdna primer were matched for detecting helicobacter genus bacteria. a hp-specific primer pair was used simultaneously to exclude the presence of hp. the pcr ...19979812529
focally enhanced gastritis: a frequent type of gastritis in patients with crohn's disease.gastric histology is not well studied in patients with crohn's disease. the aim of this study was to analyze the histological appearance of gastric mucosa in patients with crohn's disease.19979041230
inhibition of helicobacter pylori binding to gastrointestinal epithelial cells by sialic acid-containing oligosaccharides.helicobacterpylori, the ulcer pathogen residing in the human stomach, binds to epithelial cells of the gastric antrum. we have examined binding of 13 bacterial isolates to epithelial cell lines by use of a sensitive microtiter plate method in which measurement of bacterial urease activity provides the means for quantitation of bound organisms. several established human gastrointestinal carcinoma cell lines grown as monolayers were compared for suitability in these assays, and the duodenum-derive ...19979009338
helicobacter pylori culture supernatant inhibits binding and proliferative response of human gastric cells to epidermal growth factor: implications for h.pylori interference with ulcer healing?helicobacter pylori (h. pylori) infection of gastric mucosa is strongly associated with gastritis and peptic ulcer disease. however, the mechanisms of the ulcerogenic action of h. pylori and/or the interference of h. pylori with ulcer healing are unknown. through binding to its receptor, epidermal growth factor (egf) accelerates cells migration and triggers epithelial cell proliferation which are both important for the healing of gastroduodenal ulcers. h. pylori seems to interfere with ulcer hea ...19979243127
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