Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| characterization of a helicobacter hepaticus puta mutant strain in host colonization and oxidative stress. | helicobacter hepaticus is a gram-negative, spiral-shaped microaerophilic bacterium associated with chronic intestinal infection leading to hepatitis and colonic and hepatic carcinomas in susceptible strains of mice. in the closely related human pathogen helicobacter pylori, l-proline is a preferred respiratory substrate and is found at significantly high levels in the gastric juice of infected patients. a previous study of the proline catabolic puta flavoenzymes from h. pylori and h. hepaticus r ... | 2008 | 18458068 |
| probiotics, prebiotics, and synbiotics. | according to the german definition, probiotics are defined viable microorganisms, sufficient amounts of which reach the intestine in an active state and thus exert positive health effects. numerous probiotic microorganisms (e.g. lactobacillus rhamnosus gg, l. reuteri, bifidobacteria and certain strains of l. casei or the l. acidophilus-group) are used in probiotic food, particularly fermented milk products, or have been investigated--as well as escherichia coli strain nissle 1917, certain entero ... | 2008 | 18461293 |
| the effects of cyclooxygenase2-prostaglandine2 pathway on helicobacter pylori-induced urokinase-type plasminogen activator system in the gastric cancer cells. | urokinase-type plasminogen activator (upa) and its receptor (upar) play an important role in the destruction of the extracellular matrix and basement membrane. the induction of upa and upar in the gastric cancer cells with h. pylori has been demonstrated previously. the involvement of cox-2-pge2 pathway in the upa system (upa and upar) expression is unclear. | 2008 | 18466392 |
| helicobacter pylori induces beta3gnt5 in human gastric cell lines, modulating expression of the saba ligand sialyl-lewis x. | chronic helicobacter pylori infection is recognized as a cause of gastric cancer. h. pylori adhesion to gastric cells is mediated by bacterial adhesins such as sialic acid-binding adhesin (saba), which binds the carbohydrate structure sialyl-lewis x. sialyl-lewis x expression in the gastric epithelium is induced during persistent h. pylori infection, suggesting that h. pylori modulates host cell glycosylation patterns for enhanced adhesion. here, we evaluate changes in the glycosylation-related ... | 2008 | 18483624 |
| breast cancer and microbial cancer incidence in female populations around the world: a surprising hyperbolic association. | current literature on cancer epidemiology typically discusses etiology of cancer by cancer type. risks of different cancer types are, however, correlated at population level and may provide etiological clues. we showed previously an unexpected very high positive correlation between breast cancer (bc) and young-adult hodgkin disease incidence rates. in a population-based case-control study of bc, older ages at the first epstein-barr virus exposure, indicated by older ages at onset of infectious m ... | 2008 | 18537160 |
| the effects of physical and psychological stress on the gastro-intestinal tract: lessons from animal models. | physical and psychological stresses are widely accepted as triggers and / or modifiers of the clinical course of diverse gastrointestinal disorders such as peptic ulcer, irritable bowel syndrome or inflammatory bowel disease. growing experimental evidence from a variety of models such as immobilization, thermal injury or early maternal deprivation in laboratory animals uniformly supports the ability of stress to induce the development of gastric ulcers, altered gastrointestinal motility and ion ... | 2008 | 18537637 |
| [proteomic analysis of helicobacter pylori in human gastritis and gastric cancer]. | to establish 2-dimensional electrophoresis (2-de) maps of helicobac-ter pylori in human gastritis, and gastric cancer, to identify the differentially expressed proteins,and to discuss the role of bacterial factor in pathogenesis. | 2008 | 18544840 |
| [human salivary mucins--their role and meaning]. | the aim of this work is to present the current views concerning the role and the meaning of human salivary mucins. | 2007 | 18557382 |
| association of helicobacter pylori and epstein-barr virus with gastric cancer and peptic ulcer disease. | helicobacter pylori and epstein-barr virus (ebv) infections are common world-wide. though h. pylori infection is a major factor in gastroduodenal diseases, its role in association with ebv infection is unknown. we prospectively studied the association of h. pylori and ebv in patients with gastric cancer (gc) and peptic ulcer disease (pud). | 2008 | 18569983 |
| the role of infectious agents in the etiology of ocular adnexal neoplasia. | given the fact that infectious agents contribute to around 18% of human cancers worldwide, it would seem prudent to explore their role in neoplasms of the ocular adnexa: primary malignancies of the conjunctiva, lacrimal glands, eyelids, and orbit. by elucidating the mechanisms by which infectious agents contribute to oncogenesis, the management, treatment, and prevention of these neoplasms may one day parallel what is already in place for cancers such as cervical cancer, hepatocellular carcinoma ... | 2008 | 18572051 |
| effects of different helicobacter pylori culture filtrates on growth of gastric epithelial cells. | to study the effects of different helicobacter pylori (h pylori) culture filtrates on growth of gastric epithelial cells. | 2008 | 18595143 |
| cross-reactivity between immune responses to helicobacter bilis and helicobacter pylori in a population in thailand at high risk of developing cholangiocarcinoma. | helicobacter bilis dna has been detected in human tissue and is a candidate for etiologic investigations on the causes of hepatic and biliary tract diseases, but reliable serologic tests need to be developed in order to pursue such investigations. the scope of this study was to assess the specificity of two assays for h. bilis immune response allowing for h. pylori, and their cross-reactivity in a population in thailand at high risk for cholangiocarcinoma. plasma samples from 92 thai volunteers ... | 2008 | 18596203 |
| loss of imprinting of igf2 and h19, loss of heterozygosity of igf2r and ctcf, and helicobacter pylori infection in laryngeal squamous cell carcinoma. | imprinting analyses of igf2 and h19, loss of heterozygosity (loh) analyses of igf2r and ctcf and helicobacter pylori detection, were performed on 35 human laryngeal squamous cell carcinomas (lscc). forty-six percent of the tumors were heterozygous for igf2, and 54% were informative for the h19. biallelic expression of igf2 was observed in 33% (5 out of 15) of the tumors and in 27% (4 out of 15) of adjacent non-tumorous laryngeal tissues. h19 loss of imprinting (loi) was observed in 24% (4 out of ... | 2008 | 18604514 |
| gg genotype of cyclin d1 g870a polymorphism is associated with non-cardiac gastric cancer in a high-risk region of china. | cyclin d1 (ccnd1) is a regulatory protein involved in the cell cycle of both normal and neoplastic cells. polymorphism of this gene at codon 242 in exon 4 (a870g) has an impact on the risk of several human cancers. the purpose of this study was to study the relation between the ccnd1 a870g gene polymorphism and the risk of non-cardiac gastric cancer in a chinese population. | 2008 | 18609126 |
| [decrease of the prevalence of helicobacter pylori infection in the czech republic]. | helicobacter pylori (hp) is the most common chronic bacterial infection in humans. hp has been demonstrated worldwide and in individuals of all ages. the overall prevalence of hp infection has fallen dramatically in the czech republic over recent 15 years (from 70% in 1993 to 35% in 2006). this decrease can be explained mostly by the relatively favourable and improving socioeconomic conditions after the fall of communist regime. however, it is necessary to consider also the fundamental determina ... | 2008 | 18630179 |
| helicobacter pylori produces unique filaments upon host contact in vitro. | helicobacter pylori exists in 2 distinct morphological states, helicoid and coccoid. both have been observed in in vitro culture and in gastric biopsies. we visualized h. pylori during ags cell infections using immunofluorescence microscopy. anti-h. pylori mouse serum as well as human serum from h. pylori-positive patients recognized long, thin bacterial filaments, which formed on helicoids and more frequently on coccoids. these filaments reached lengths of 59 microm and often connected bacteria ... | 2008 | 18641700 |
| the relationship between updated sydney system score and ldl cholesterol levels in patients infected with helicobacter pylori. | helicobacter pylori (h. pylori) chronically infects the human stomach and may cause extra-gastrointestinal diseases. the role of h. pylori in the pathogenesis of atherosclerosis and its effect on lipids remains controversial. the aim of this study was to examine lipid levels in patients with and without h. pylori infection. | 2009 | 18649137 |
| [gastric lymphoma. morphological and immunohistochemical study]. | most of extranodal lymphomas are localized in gastrointestinal tract, gastric lymphoma representing more than 50% of them. the role that helicobacter pylori (h. pylori) plays in pathogenesis of gastric lymphoma has changed the therapeutic approach. | 2008 | 18677925 |
| expression, purification, crystallization and preliminary x-ray analysis of an nad-dependent glyceraldehyde-3-phosphate dehydrogenase from helicobacter pylori. | helicobacter pylori is a dangerous human pathogen that resides in the upper gastrointestinal tract. little is known about its metabolism and with the onset of antibiotic resistance new treatments are required. in this study, the expression, purification, crystallization and preliminary x-ray diffraction of an nad-dependent glyceraldehyde-3-phosphate dehydrogenase from h. pylori are reported. | 2008 | 18678942 |
| targeting focal adhesions:helicobacter pylori-host communication in cell migration. | abstract: highly dynamic integrin-based focal adhesions provide an important structural basis for anchoring the cellular actin cytoskeleton to the surrounding extracellular matrix. the human pathogen helicobacter pylori (h. pylori) directly targets integrins with drastic consequences on the epithelial cell morphology and migration, which might contribute to the disruption of the gastric epithelium in vivo. in this review, we summarize the recent findings concerning the complex mechanism through ... | 2008 | 18684322 |
| cyp2c19 genotypes and helicobacter pylori eradication. | 2008 | 18699976 | |
| multiple early gastric cancer with gastritis cystica profunda showing various histological types. | we report a case of multiple early gastric cancer showing varied histological types associated with gastritis cystica profunda (gcp). a 61-year-old man who had early gastric cancer associated with gcp underwent a distal gastrectomy with lymphadenectomy. histological examination showed various histological types of cancer -well differentiated, moderately differentiated, poorly differentiated adenocarcinoma, mucinous adenocarcinoma and signet ring cell carcinoma- that had developed independently i ... | 2008 | 18705349 |
| parp-1 val762ala polymorphism, caga+ h. pylori infection and risk for gastric cancer in han chinese population. | parp-1 plays important role in the ber (base excision repair) and maintenance of genomic integrity. previous study found the val762ala genetic variant in the parp-1 gene contributed to susceptibility of some cancers and decreased parp-1 enzyme activity in response to oxidative damage. helicobacter pylori (h. pylori) infection was thought to be one of the major causes of gastric cancer. in this study, we investigated the association between the parp-1 val762ala polymorphism, caga(+) h. pylori inf ... | 2009 | 18716896 |
| hancornia speciosa: indications of gastroprotective, healing and anti-helicobacter pylori actions. | mangaba (hancornia speciosa gomez) is a medicinal plant frequently cited in ethnopharmacological inventories of the central region of brazil against gastrointestinal disorders such as diarrhoea, ulcer, gastritis and stomach ache. | 2008 | 18761076 |
| zn2+-linked dimerization of ureg from helicobacter pylori, a chaperone involved in nickel trafficking and urease activation. | the biosynthesis of the active metal-bound form of the nickel-dependent enzyme urease involves the formation of a lysine-carbamate functional group concomitantly with the delivery of two ni(2+) ions into the precast active site of the apoenzyme and with gtp hydrolysis. in the urease system, this role is performed by ureg, an accessory protein belonging to the group of homologous p-loop gtpases, often required to complete the biosynthesis of nickel-enzymes. this study is focused on ureg from heli ... | 2009 | 18767150 |
| helicobacter pylori suppresses glycogen synthase kinase 3beta to promote beta-catenin activity. | the human pathogen helicobacter pylori influences cell adhesion, proliferation, and apoptosis and is involved in gastric adenocarcinoma formation. in our study we analyzed the impact of h. pylori infection on the regulation of beta-catenin, which plays a central role in both cell adhesion and tumorigenesis. infection of madin-darby canine kidney cells with h. pylori led to suppression of ser/thr phosphorylation and ubiquitin-dependent degradation of beta-catenin and to up-regulation of lymphoid ... | 2008 | 18772141 |
| recombination-based in vivo expression technology identifies helicobacter pylori genes important for host colonization. | here we undertook to identify colonization and gastric disease-promoting factors of the human gastric pathogen helicobacter pylori as genes that were induced in response to the stomach environment. using recombination-based in vivo expression technology (rivet), we identified six promoters induced in the host compared to laboratory conditions. three of these promoters, designated pivi10, pivi66, and pivi77, regulate genes that h. pylori may use to interact with other microbes or the host. pivi10 ... | 2008 | 18794279 |
| helicobacter pylori protein hp0175 transactivates epidermal growth factor receptor through tlr4 in gastric epithelial cells. | the pathophysiology of helicobacter pylori-associated gastroduodenal diseases, ulcerogenesis, and carcinogenesis is intimately linked to activation of epidermal growth factor receptor (egfr) and production of vascular endothelial growth factor (vegf). extracellular virulence factors, such as caga and vaca, have been proposed to regulate egfr activation and vegf production in gastric epithelial cells. we demonstrate that the h. pylori secretory protein, hp0175, by virtue of its ability to bind tl ... | 2008 | 18806258 |
| evaluation of the clinical significance of homb, a novel candidate marker of helicobacter pylori strains associated with peptic ulcer disease. | homb codes for a putative helicobacter pylori outer membrane protein and has previously been associated with peptic ulcer disease (pud) in children. | 2008 | 18811585 |
| role of cyclooxygenase-2 functional gene polymorphisms in helicobacter pylori induced gastritis and gastric atrophy. | in india, the role of host genetic factors is poorly studied for helicobacter pylori associated diseases. therefore, we evaluated the association of functionally relevant cox-2 gene polymorphisms (-765 g>c and +8473 t>c) in gastritis and precancerous lesions susceptibility. after upper gi endoscopy, 130 rapid urease test positive patients with non-ulcer dyspepsia, also showed positivity for h. pylori using modified geimsa staining and anti-caga igg serology were included. all patients and 260 as ... | 2009 | 18825488 |
| effect of helicobacter pylori eradication on oncogenes and cell proliferation. | helicobacter pylori , the main cause of chronic gastritis, is a class 1 gastric carcinogen. however, it remains unclear whether h. pylori affects molecular alterations in chronic gastritis. thus, this study was designed to investigate the effect of h. pylori eradication on the expression of human telomerase rna (htr), human telomerase reverse transcriptase (htert), c-myc and proliferation nuclear cell antigen (pcna) in h. pylori associated chronic gastritis. | 2008 | 18837738 |
| probiotics and small bowel mucosa: molecular aspects of their interactions. | probiotics are described as "friendly bacteria" that could improve the intestine defense by interacting with the resident microflora. there is a large body of evidence suggesting that consumption of functional food containing probiotics exerts positive effects on human health. several clinical trials have highlighted the efficiency of probiotics in the prevention and treatment of different gastrointestinal disorders including the prevention of antibiotic associated diarrhea, the remission in pat ... | 2006 | 18850204 |
| molecular expression of gastrokine 1 in normal mucosa and in helicobacter pylori-related preneoplastic and neoplastic gastric lesions. | we have analyzed the expression of gastrokine 1 (gkn1), one of the most abundant protein of gastric mucosa, in helicobacter pylori-related preneoplastic and neoplastic gastric lesions. the gkn1 expression was downregulated in 36 h. pylori-positive patients with respect to 29 h. pylori-negative subjects as evaluated by western blot, reverse transcriptase polymerase chain reaction (rt-pcr) and immunohistochemistry, showing a progressive decrease from chronic gastritis to atrophy and intestinal met ... | 2008 | 18927498 |
| effective prevention and treatment of helicobacter pylori infection using a combination of catechins and sialic acid in ags cells and balb/c mice. | the increasing emergence of helicobacter pylori strains resistant to antibiotics may cause unsuccessful treatment. an alternative agent or mixture with anti-h. pylori effect is urgently required to reduce h. pylori infection. we explored the preventive and therapeutic potential of a combination of catechins and sialic acid on h. pylori-infected human gastric cells in vitro and in mice in vivo. we evaluated the anti-h. pylori activity of catechins and/or sialic acid using the agar dilution and ch ... | 2008 | 18936202 |
| a case of cronkhite-canada syndrome: remission after treatment with anti-helicobacter pylori regimen. | a 67-year-old man with nausea, appetite loss, frequent diarrhea and severe weight loss presented with alopecia, skin hyperpigmentation and onychodystrophy. laboratory investigations showed mild anemia, hypoproteinemia and hypoalbuminemia. colonoscopy identified the numerous, hyperemic and sessile polyps with mucous exudation of various sizes throughout the colorectum. the ileocecal valve was substantially swollen. magnified chromoendoscopy revealed sparsely distributed crypt openings with wideni ... | 2008 | 18948692 |
| [establishment of mongolian gerbil model of long-term helicobacter pylori infection]. | to establish a model of long-term infection with helicobacter pylori (hp) in mongolian gerbil (meriones unguiculatus), and to investigate if hp combined with n-methyl-n'-nitro-n-nitrosoguanidine (mnng) has a synergistic effect to induce gastric mucosa injury. to investigate pathological changes of gastric mucosa during long-term hp infection in mongolian gerbil model. | 2008 | 18956630 |
| [effects of tumor necrosis factor-alpha inducing protein-alpha secreted by helicobacter pylori on human gastric epithelial cells]. | to investigate the effects of tumor necrosis factor-alpha-inducing protein-alpha (tipalpha) released from helicobacter pylori (hp) on human gastric epithelial cells. | 2008 | 18956632 |
| aetiology of cancer in asia. | cancer has become the leading cause of death in many asian countries. there is an increasing trend in breast, prostate and colon cancers, which are considered as typical of economically developed countries. although breast and prostate cancer rates are still lower than in western countries, they are particularly rapidly increasing. in this paper, we review recently published literature to identify important etiologic factors affecting the cancer risk in asian populations. infectious agents such ... | 2008 | 18990005 |
| infectious agents and colorectal cancer: a review of helicobacter pylori, streptococcus bovis, jc virus, and human papillomavirus. | based on the high volume of bacteria and viruses that the intestine is exposed to and the importance of infectious agents in some gastrointestinal and anogenital cancers, it is not surprising the many studies have evaluated the association between colorectal cancer and infectious agents. this review highlights investigations of four agents in relation to colorectal cancer. helicobacter pylori, streptococcus bovis, jc virus, and human papillomavirus have all been evaluated as possible etiologic a ... | 2008 | 18990738 |
| cdx2 expression is induced by helicobacter pylori in ags cells. | 2009 | 18991163 | |
| causality and gastrointestinal infections: koch, hill, and crohn's. | the gastrointestinal tract contains a complex mix of microorganisms. therefore, the finding of a particular microbe in the gastrointestinal tract or stools of a patient with intestinal disease does not necessarily indicate that the clinical state resulted from the presence of this organism. for this reason, extending from epidemiological association to causality is particularly challenging for gastrointestinal diseases. most established agents of acute bacterial gastroenteritis (eg, escherichia ... | 2008 | 18992408 |
| helicobacter pylori mutants defective in the clpp atp-dependant protease and the chaperone clpa display reduced macrophage and murine survival. | the atp-dependent caseinolytic proteases (clp) are important in resistance against environmental stresses, antibiotic treatments and host immune defences for a number of pathogenic bacteria. clpp is the proteolytic subunit, whilst clpa acts both as a chaperone and as an atpase driving the degradation of damaged or mis-made proteins. the gastric pathogen helicobacter pylori infects approximately half of the world's population and can cause gastric or duodenal ulcers, gastric malignancies and muco ... | 2009 | 18992803 |
| helicobacter pylori vaca-induced inhibition of gsk3 through the pi3k/akt signaling pathway. | helicobacter pylori vaca toxin contributes to the pathogenesis and severity of gastric injury. we found that incubation of az-521 cells with vaca resulted in phosphorylation of protein kinase b (akt) and glycogen synthase kinase-3beta (gsk3beta) through a pi3k-dependent pathway. following phosphorylation and inhibition of gsk3beta,beta-catenin was released from a gsk3beta/beta-catenin complex, with subsequent nuclear translocation. methyl-beta-cyclodextrin (mcd) and phosphatidylinositol-specific ... | 2009 | 18996844 |
| [assessment of rate of hydroxyl anions production by helicobacter pylori in stomach]. | production of hydroxyl anions by tissue samples of pylorus mucous membrane obtained from 45 patients with gastric or duodenal ulcers was investigated. the production was estimated using the recently developed method based on measurement of rate of ph change in urea-containing reaction mixture. the rate of [oh-] generation as a result of h. pylori metabolism accounted on pylorus square varied from 0.4 to 1318.9 mcmol [oh-]/min, and in 90.2% of cases it did not exceed 128.1 mcmol [oh-]/min. this r ... | 2008 | 19004294 |
| h. pylori selectively blocks egfr endocytosis via the non-receptor kinase c-abl and caga. | helicobacter pylori infection is a primary cause of peptic ulcers and is associated with gastric carcinogenesis. the h. pylori-induced pathophysiology may be linked to the deregulation of egfr signalling. elevated mucosal levels of egf and the egfr have been found in antral gastric biopsies of h. pylori-infected patients. a critical mechanism for regulating egfr signalling is ligand-induced endocytosis. the internalized receptor recycles back to the plasma membrane for continued signalling or is ... | 2009 | 19016792 |
| mosaic dna imports with interspersions of recipient sequence after natural transformation of helicobacter pylori. | helicobacter pylori colonizes the gastric mucosa of half of the human population, causing gastritis, ulcers, and cancer. h. pylori is naturally competent for transformation by exogenous dna, and recombination during mixed infections of one stomach with multiple h. pylori strains generates extensive allelic diversity. we developed an in vitro transformation protocol to study genomic imports after natural transformation of h. pylori. the mean length of imported fragments was dependent on the combi ... | 2008 | 19030104 |
| interaction between cd14-159c>t polymorphism and helicobacter pylori is associated with serum total immunoglobulin e. | total serum ige is regulated by both environmental and genetic factors. association and linkage studies have suggested a role of cd14-159c>t polymorphism in the regulation of serum total ige, but the results have been contradictory. it seems that gene-environment interactions are involved in this regulation. | 2008 | 19037967 |
| importance of egf receptor, her2/neu and erk1/2 kinase signalling for host cell elongation and scattering induced by the helicobacter pylori caga protein: antagonistic effects of the vacuolating cytotoxin vaca. | helicobacter pylori is the causative agent of gastric pathologies ranging from chronic gastritis to peptic ulcers and even cancer. virulent strains carrying both the cag pathogenicity island (cagpai) and the vacuolating cytotoxin vaca are key players in disease development. the cagpai encodes a type iv secretion system (t4ss) which forms a pilus for injection of the caga protein into gastric epithelial cells. injected caga undergoes tyrosine phosphorylation and induces actin-cytoskeletal rearran ... | 2009 | 19046339 |
| does the expression of cyclin e, prb, and p21 correlate with prognosis in gastric adenocarcinoma? | cyclin e is a protein that plays a key role in the g1 --> s transition of the normal cell cycle. the product of retinoblastoma gene (prb) is the master regulator of entry into the cell cycle and p21 protein is a cyclin-dependent kinase inhibitor that disturbs the progression through the cell cycle. the expression of these proteins, among many others, is being deregulated in tumorogenesis. the aim of this study was to investigate whether cyclin e, prb, and p21 can be used as prognostic indicators ... | 2009 | 19058005 |
| ulcers and gastritis. | 2009 | 19058127 | |
| tight junctions as targets of infectious agents. | the epithelial barrier is a critical border that segregates luminal material from entering tissues. essential components of this epithelial fence are physical intercellular structures termed tight junctions. these junctions use a variety of transmembrane proteins coupled with cytoplasmic adaptors, and the actin cytoskeleton, to attach adjacent cells together thereby forming intercellular seals. breaching of this barrier has profound effects on human health and disease, as barrier deficiencies ha ... | 2009 | 19059200 |
| role of the gastrin-gastrin receptor system in the expansive growth of human gastric neoplasms. | gastrin is one of the most important gut hormones. however, the role of the gastrin-gastrin receptor (gr) system in the growth of gastric tumors is still unclear. | 2008 | 19065055 |
| [the change of cyclooxygenase-2 and inducible nitric oxide synthase in the gastric mucosa one year after eradication of helicobacter pylori]. | the cyclooxygenase-2 (cox-2) and inducible nitric oxide synthase (inos), the proteins that have the role in the gastric carcinogenesis, are stimulated by h. pylori infection in the gastric mucosa. the aim of this study was to evaluate the expression of cox-2 and inos proteins one year after the eradication of h. pylori. | 2008 | 19077474 |
| novel single nucleotide polymorphism of the vegf gene as a risk predictor for gastroduodenal ulcers. | the gastroduodenal ulcer (gdu) mostly develops on the lesser curvature side of stomach and the anterior wall of duodenal bulb, irrespective of the etiologies including helicobacter pylori infection, non-steroidal anti-inflammatory drugs, alcohol, etc. however, a clear explanation as to why ulcers are prevalent in these areas has still not been given. the current study was designed to evaluate whether the vascular endothelial growth factor (vegf) polymorphism could predict susceptibility to gdu t ... | 2008 | 19120886 |
| expression of inducible nitric oxide synthase and nitric oxide-modified proteins in helicobacter pylori-associated atrophic gastric mucosa. | induction of inducible nitric oxide synthase (inos) may be involved in carcinogenesis of the stomach, because nitric oxide (no) derived from inos can exert dna damage and post-transcriptional modification of target proteins. in the present study, we investigated the correlation between endoscopic findings and inos mrna expression/no-modified proteins in the gastric mucosa. | 2008 | 19120907 |
| the influence of the eradication of helicobacter pylori on gastric ghrelin, appetite, and body mass index in patients with peptic ulcer disease. | helicobacter pylori (h. pylori) infection has been known to influence the gastric leptin and ghrelin secretion, for which the exact pathogenic role has not been documented yet. this study was designed to investigate the influence of h. pylori eradication on plasma or gastric levels of ghrelin, body mass index (bmi), and resultant levels of appetite in patients with peptic ulcer disease. | 2008 | 19120912 |
| identification of small noncoding rnas in helicobacter pylori by a bioinformatics-based approach. | small noncoding rnas (srnas) are a group of regulatory rna molecules normally without a protein-coding function. in recent years, the importance of srnas as mediators of gene expression in bacteria has begun to be recognized. more than 70 srnas have been known in escherichia coli. however, little is known about srnas in helicobacter pylori, a human pathogen associated with gastric diseases. here, we systematically identified srnas in the h. pylori genome by a computational approach based on gene ... | 2009 | 19123032 |
| genome sequence analysis of helicobacter pylori strains associated with gastric ulceration and gastric cancer. | persistent colonization of the human stomach by helicobacter pylori is associated with asymptomatic gastric inflammation (gastritis) and an increased risk of duodenal ulceration, gastric ulceration, and non-cardia gastric cancer. in previous studies, the genome sequences of h. pylori strains from patients with gastritis or duodenal ulcer disease have been analyzed. in this study, we analyzed the genome sequences of an h. pylori strain (98-10) isolated from a patient with gastric cancer and an h. ... | 2009 | 19123947 |
| expression of helicobacter pylori caga domains by library-based construct screening. | highly pathogenic strains of helicobacter pylori use a type iv secretion system to inject the caga protein into human gastric cells. there, caga associates with the inner side of the membrane and is tyrosine-phosphorylated at epiya motifs by host kinases. the phosphorylation triggers a series of interactions between caga and human proteins that result in a dramatic change of cellular morphology. structural and functional analyses of the protein have proved difficult, due to the proteolytically s ... | 2009 | 19143840 |
| helicobacter pylori caga phosphorylation-independent function in epithelial proliferation and inflammation. | caga, a major virulence factor of helicobacter pylori (hp), is delivered into gastric epithelial cells and exists in phosphorylated and nonphosphorylated forms. the biological activity of the phosphorylated form is well established; however, function(s) of the nonphosphorylated form remain elusive. here, we report that a conserved motif in the c-terminal region of caga, which is distinct from the epiya motifs used for phosphorylation and which we designate crpia (conserved repeat responsible for ... | 2009 | 19154985 |
| the peopling of the pacific from a bacterial perspective. | two prehistoric migrations peopled the pacific. one reached new guinea and australia, and a second, more recent, migration extended through melanesia and from there to the polynesian islands. these migrations were accompanied by two distinct populations of the specific human pathogen helicobacter pylori, called hpsahul and hspmaori, respectively. hpsahul split from asian populations of h. pylori 31,000 to 37,000 years ago, in concordance with archaeological history. the hpsahul populations in ne ... | 2009 | 19164753 |
| effect of helicobacter pylori's vacuolating cytotoxin on the autophagy pathway in gastric epithelial cells. | host cell responses to helicobacter pylori infection are complex and incompletely understood. here, we report that autophagy is induced within human-derived gastric epithelial cells (ags) in response to h. pylori infection. these autophagosomes were distinct and different from the large vacuoles induced during h. pylori infection. autophagosomes were detected by transmission electron microscopy, conversion of lc3-i to lc3-ii, gfp-lc3 recruitment to autophagosomes, and depended on atg5 and atg12. ... | 2009 | 19164948 |
| h. pylori-induced apoptosis in human gastric cancer cells mediated via the release of apoptosis-inducing factor from mitochondria. | our previous study of helicobacter pylori-induced apoptosis showed the involvement of bcl-2 family proteins and cytochrome c release from mitochondria. here, we examine the release of other factors from mitochondria, such as apoptosis-inducing factor (aif), and upstream events involving caspase-8 and bid. | 2008 | 19166416 |
| [correlation of helicobacter pylori infection with the expression of cox-2 and egfr and vegf in human gastric carcinoma]. | to investigate the correlation of helicobactor pylori (hp) infection with the expression of cox-2, egfr and vegf in human gastric carcinoma. | 2008 | 19173907 |
| methylation of p16ink4a and mitotic arrest defective protein 2 (mad2) genes in gastric marginal-zone b-cell lymphomas. | products of cyclin-dependent kinase inhibitor p16(ink4a)and mitotic arrest defective protein 2 (mad2) genesare key regulator proteins at the g1 restriction point and mitotic checkpoint of the cell cycle. the objective of this study was to investigate the role of promoter methylation of p16(ink4a) and mad2 genes in gastric marginal-zone b-cell lymphoma (mzbcl). | 2009 | 19174606 |
| cross-reaction of antibody against helicobacter pylori urease b with platelet glycoprotein iiia and its significance in the pathogenesis of immune thrombocytopenic purpura. | many clinical investigations have suggested that helicobacter pylori (h. pylori) infection might be associated with immune thrombocytopenic purpura (itp), but its role in the pathogenesis of itp is unsettled. in this study, we cultured h. pylori, produced recombinant h. pylori urease (ure) b, and then prepared monoclonal antibody (moab) against ureb, 1f11, both 1f11 and moab against human platelet glycoprotein (gp) iiia, sz21, could bind to the band of gp iiia of normal platelet lysate, but not ... | 2009 | 19184277 |
| chronic aspirin use suppresses cdh1 methylation in human gastric mucosa. | there have been reports showing a protective role of aspirin and other nonsteroidal anti-inflammatory drugs (nsaids) against gastrointestinal cancers. e-cadherin (cdh1) is an adhesion molecule involved in tumour invasion/metastasis. silencing of cdh1 by promoter cpg island methylation was shown in gastric cancer, precancerous lesion, and helicobacter pylori-infected chronic gastritis. we investigated the methylation status of cdh1 in noncancerous gastric mucosa in chronic aspirin user, and asses ... | 2010 | 19184424 |
| expression of beta-defensin 4 is increased in human gastritis. | infection with helicobacter pylori (h. pylori) leads to the initiation of innate immune responses with increased antimicrobial peptide (amp) expression in the gastric epithelium. this study aimed to determine the expression of the novel peptides beta-defensin 4 (hbd-4) and rnase 7 in infectious and non-infectious gastritis. furthermore, pattern recognition receptors and mechanisms of regulation were characterized. | 2009 | 19200166 |
| cancer prevention by vaccination against hepatitis b. | chronic inflammation caused by persistent infection is closely related to a number of cancers; these include hepatitis b (hbv) or c and hepatoma, human papilloma virus and cervical cancer, and helicobacter pylori and gastric cancer. the first evidence of cancer prevention by vaccination in humans was provided by hbv vaccination in infants. chronic hbv is related to approximately 60%-90% of hepatocellular carcinomas (hcc) in adults and nearly 100% of childhood hcc in areas endemic for hbv infecti ... | 2009 | 19213561 |
| helicobacter pylori, asthma and allergy. | bronchial asthma and allergic diseases are orchestrated by t-cells producing t-helper type 2 (th2) cytokines, such as interleukin-4 (il-4) and il-5, and are inhibited by th1 responses. helicobacter pylori has chronically infected the human population for c. 100,000 years and preferentially elicits a th1 mucosal immune response with the production of interferon-gamma and il-12. among several bacterial factors, the neutrophil-activating protein of h. pylori (hp-nap) not only plays a key role in dr ... | 2009 | 19220467 |
| differences in genome content among helicobacter pylori isolates from patients with gastritis, duodenal ulcer, or gastric cancer reveal novel disease-associated genes. | helicobacter pylori establishes a chronic infection in the human stomach, causing gastritis, peptic ulcer, or gastric cancer, and more severe diseases are associated with virulence genes such as the cag pathogenicity island (pai). the aim of this work was to study gene content differences among h. pylori strains isolated from patients with different gastroduodenal diseases in a mexican-mestizo patient population. h. pylori isolates from 10 patients with nonatrophic gastritis, 10 patients with du ... | 2009 | 19237517 |
| therapeutic strategies for hepatitis- and other infection-related immune thrombocytopenias. | secondary thrombocytopenia may result from autoimmune diseases, lymphoproliferative disorders, infections, myelodysplastic syndromes, common variable immunodeficiency, agammaglobulinemia, hypogammaglobulinemia, immunoglobulin a deficiency, and drugs. the presence of thrombocytopenia may result from chronic infections with hepatitis c virus (hcv), human immunodeficiency virus (hiv), and helicobacter pylori and should be considered in the differential diagnosis of immune thrombocytopenic purpura ( ... | 2009 | 19245929 |
| epidermal growth factor receptor activation protects gastric epithelial cells from helicobacter pylori-induced apoptosis. | helicobacter pylori infection disrupts the balance between gastric epithelial cell proliferation and apoptosis, which is likely to lower the threshold for the development of gastric adenocarcinoma. h pylori infection is associated with epidermal growth factor (egf) receptor (egfr) activation through metalloproteinase-dependent release of egfr ligands in gastric epithelial cells. because egfr signaling regulates cell survival, we investigated whether activation of egfr following h pylori infectio ... | 2009 | 19250983 |
| extracellular and intracellular pattern recognition receptors cooperate in the recognition of helicobacter pylori. | helicobacter pylori infects half of the world's population, thereby causing significant human morbidity and mortality. the mechanisms by which professional antigen-presenting cells recognize the microbe are poorly understood. | 2009 | 19272387 |
| in this issue: proteomics 6/2009. | in this issue of proteomics you will find the following highlighted articles:keeping up with the lung cancersyou're in good company if you smoke and develop lung cancer. the world health organization estimates 1.2 million new cases occur every year. on the other hand, 1.1 million people die from it every year-bummer. one reason for the high death rate is the frequent development of resistance to several of the most commonly used drugs simultaneously. multiple drug resistance (mdr) is the major c ... | 2009 | 19294620 |
| single nucleotide polymorphism of interleukin-1beta associated with helicobacter pylori infection in immune thrombocytopenic purpura. | to examine the role of genetic factors in development of immune thrombocytopenic purpura (itp) in association with helicobacter pylori infection, gene polymorphisms within the loci for human leukocyte antigen class ii, interleukin (il)-1beta (-511), tumor necrosis factor-beta (+252), immunoglobulin (ig)g1 heavy chain (+643), and igkappa light chain (+573) were determined in 164 adults with itp and 75 healthy controls. of these gene polymorphisms, the il-1beta (-511) t allele was less frequently ... | 2009 | 19317746 |
| childhood leukemias and other hematopoietic malignancies: interdependence between an infectious event and chromosomal modifications. | a large number of epidemiological observations suggest an infectious origin of hematopoietic malignancies, including various forms of leukemia, nonhodgkin's lymphomas, hodgkin's lymphomas and multiple myelomas. incidence of nonhodgkin's lymphomas and hodgkin's lymphomas, although not of leukemias, is substantially increased under immunosuppression. specific chromosomal modifications (translocations) resulting in fusion genes frequently emerge as early, but not sufficient, events for malignant pr ... | 2009 | 19330827 |
| an african perspective on helicobacter pylori: prevalence of human infection, drug resistance, and alternative approaches to treatment. | helicobacter pylori is a gram-negative, micro-aerophilic, motile, curved rod that inhabits the gastric mucosa of the human stomach. it chronically infects thousands of millions of people world-wide, and is one of the most genetically diverse of bacterial species. infection with the bacterium leads to chronic gastritis, peptic ulceration, gastric cancers and gastric mucosa-associated lymphoid-tissue (malt) lymphoma. the prevalence of infection appears to be partly determined by geographical and s ... | 2009 | 19341534 |
| helicobacter-based mouse models of digestive system carcinogenesis. | animal models are necessary to reproduce the complex host, microbial and environmental influences associated with infectious carcinogenesis of the digestive system. today, mouse models are preferred by most researchers because of cost efficiencies, rapid reproduction, choice of laboratory reagents, and availability of genetically engineered mutants to study specific gene functions in vivo. mouse models have validated the once-provocative hypothesis that helicobacter pylori infection is a major r ... | 2009 | 19347301 |
| an unusual mechanism of thymidylate biosynthesis in organisms containing the thyx gene. | biosynthesis of the dna base thymine depends on activity of the enzyme thymidylate synthase to catalyse the methylation of the uracil moiety of 2'-deoxyuridine-5'-monophosphate. all known thymidylate synthases rely on an active site residue of the enzyme to activate 2'-deoxyuridine-5'-monophosphate. this functionality has been demonstrated for classical thymidylate synthases, including human thymidylate synthase, and is instrumental in mechanism-based inhibition of these enzymes. here we report ... | 2009 | 19370033 |
| analysis of bacterial proteins by 2de. | two-dimensional gel electrophoresis (2de) is a key analytical method for investigating bacterial -proteomes. the relatively simple genomes of many bacteria combined with only limited post--translational modifications of bacterial proteins mean that a significant proportion of the proteome is open to analysis by 2de. the applications of 2de in the field of microbiology are diverse and range from analysing physiological responses of the bacteria to environmental stress to investigating bacterial p ... | 2009 | 19381581 |
| dual nuclease and helicase activities of helicobacter pylori addab are required for dna repair, recombination, and mouse infectivity. | helicobacter pylori infection of the human stomach is associated with disease-causing inflammation that elicits dna damage in both bacterial and host cells. bacteria must repair their dna to persist. the h. pylori addab helicase-exonuclease is required for dna repair and efficient stomach colonization. to dissect the role of each activity in dna repair and infectivity, we altered the adda and addb nuclease (nuc) domains and the adda helicase (hel) domain by site-directed mutagenesis. extracts of ... | 2009 | 19395381 |
| a single nucleotide change affects fur-dependent regulation of sodb in h. pylori. | helicobacter pylori is a significant human pathogen that has adapted to survive the many stresses found within the gastric environment. superoxide dismutase (sodb) is an important factor that helps h. pylori combat oxidative stress. sodb was previously shown to be repressed by the ferric uptake regulator (fur) in the absence of iron (apo-fur regulation) [1]. herein, we show that apo regulation is not fully conserved among all strains of h. pylori. apo-fur dependent changes in sodb expression are ... | 2009 | 19399190 |
| dual infection system identifies a crucial role for pka-mediated serine phosphorylation of the epec-tir-injected effector protein in regulating rac1 function. | many gram-negative pathogenic bacteria possess type-iii or type-iv secretion systems to inject 'effector' proteins directly into host cells to modulate cellular processes in their favour. a common target is the actin-cytoskeleton linked to the delivery of a single (caga) effector by helicobacter pylori and multiple effectors by enteropathogenic escherichia coli (epec) respectively. here we report co-infection as a powerful strategy for defining effector protein function and mechanisms by which t ... | 2009 | 19438518 |
| serum bactericidal activity against helicobacter pylori in patients with hypogammaglobulinaemia. | the two major primary antibody deficiency disorders are x-linked hypogammaglobulinaemia (xla) and common variable immunodeficiency (cvid). cvid patients have an elevated risk for gastric cancer and extra-nodal marginal zone lymphoma. both diseases are associated with helicobacter pylori infection. we investigated whether antibody deficiency leads to defective serum bactericidal activity against h. pylori. we also investigated the correlation with immunoglobulin (ig)m levels and observed the term ... | 2009 | 19438595 |
| adult onset schönlein-henoch purpura associated with helicobacter pylori infection. | schönlein-henoch purpura (shp) is a systemic vasculitis, primarily involving the skin, gastrointestinal (gi) tract, joints, and kidneys. a wide variety of different conditions may be implicated in the pathogenesis of shp. we report a 33-year-old man who presented with shp accompanied by gastric helicobacter pylori (hp) infection. the gi manifestations and purpuric rashes were dramatically resolved after hp eradication therapy. to date, very few publications have focused on the possible pathogene ... | 2009 | 19443983 |
| aneuploidy involving chromosome 1 may be an early predictive marker of intestinal type gastric cancer. | intestinal type gastric cancer is a significant cause of mortality, therefore a better understanding of its molecular basis is required. we assessed if either aneuploidy or activity of the oncogenic transcription factor nuclear factor kappa b (nf-kappab), increased incrementally during pre-malignant gastric histological progression and also if they correlated with each other in patient samples, as they are both induced by oxygen free radicals. in a prospective study of 54 (aneuploidy) and 59 (nf ... | 2009 | 19481101 |
| involvement of ras and ap-1 in helicobacter pylori-induced expression of cox-2 and inos in gastric epithelial ags cells. | helicobacter pylori (h. pylori) is an important risk factor for chronic gastritis, peptic ulcer, and gastric cancer. the genetic differences of h. pylori isolates play a role in the clinical outcome of the infection. inflammatory genes including cyclooxygenase-2 (cox-2) and inducible nitric oxide synthase (inos) are involved in h. pylori gastritis. transcription factor ap-1 is composed of c-fos and c-jun and mediates inflammation and carcinogenesis. ras acts as a regulator for ap-1 activation in ... | 2010 | 19495976 |
| a genetic variant of the p22phox component of nadph oxidase c242t is associated with reduced risk of functional dyspepsia in helicobacter pylori-infected japanese individuals. | although inflammatory changes in the gastric mucosa are commonly observed in japanese patients with functional dyspepsia (fd), detailed data regarding the relationship between the genetic regulatory factors of inflammation and fd are not available. superoxide has been implicated in the pathogenesis of helicobacter pylori-related diseases through inflammation. nicotinamide adenine dinucleotide phosphate oxidase, a major source of superoxide generation plays a critical role in h. pylori-related ga ... | 2009 | 19531958 |
| late reactivation of sonic hedgehog by helicobacter pylori results in population of gastric epithelial cells that are resistant to apoptosis: implication for gastric carcinogenesis. | as much as that a disturbance of tissue homeostasis through dysregulated apoptosis is generally associated with carcinogenesis, gastric carcinogenesis after helicobacter pylori infection could be the accumulated consequence of imbalances between apoptosis and proliferation. since sonic hedgehog (shh) has been reported to play versatile roles in various tumorigenesis, we hypothesized that late reactivation of sonic hedgehog by h. pylori infection results in population of gastric epithelial cells ... | 2010 | 19540662 |
| associations of a ptpn11 g/a polymorphism at intron 3 with helicobactor pylori seropositivity, gastric atrophy and gastric cancer in japanese. | previous studies have revealed the significance of helicobacter pylori (h. pylori) infection as a risk factor of gastric cancer. cytotoxin-associated gene a (caga) positivity has been demonstrated to determine the clinical outcome of h. pylori infection in the presence of shp-2 (src homology 2 domain-containing protein tyrosine phosphatase-2). this study aimed to examine the formerly reported association of g/a ptpn11 (protein-tyrosine phosphatase, nonreceptor-type 11) polymorphism (rs2301756) w ... | 2009 | 19589142 |
| lansoprazole, a proton pump inhibitor, suppresses production of tumor necrosis factor-alpha and interleukin-1beta induced by lipopolysaccharide and helicobacter pylori bacterial components in human monocytic cells via inhibition of activation of nuclear factor-kappab and extracellular signal-regulated kinase. | pathogenic bacterial components play critical roles in initiation of gastrointestinal inflammation via activation of intracellular signaling pathways which induce proinflammatory cytokines such as tumor necrosis factor (tnf)-alpha and interleukin (il)-1beta. lansoprazole (lanso), a proton pump inhibitor, has been widely used for the treatment of peptic ulcers and reflux esophagitis due to its potent acid-suppressive effect. it has also been reported to have anti-inflammatory effects. in this stu ... | 2009 | 19590712 |
| influences of cyp2c19 polymorphism on recurrence of reflux esophagitis during proton pump inhibitor maintenance therapy. | patients with erosive gastroesophageal reflux disease (gerd) have rapid recurrence after treatment withdrawal. the aim is to study the influences of cyp2c19 polymorphism on recurrence of gerd during proton pump inhibitor maintenance therapy. | 2009 | 19621685 |
| diet synergistically affects helicobacter pylori-induced gastric carcinogenesis in nonhuman primates. | gastric cancer results from a combination of helicobacter pylori (h pylori) infection, exposure to dietary carcinogens, and predisposing genetic make-up. because the role of these factors in gastric carcinogenesis cannot be determined readily in human beings, the present study examined the role of an oral carcinogen and h pylori infection in rhesus monkeys. | 2009 | 19622359 |
| a comparison of glycan expression and adhesion of mouse-adapted strains and clinical isolates of helicobacter pylori. | adherence to the mucosal surface, essential for successful colonization of the gastric mucosa by the human pathogen helicobacter pylori, is predominantly mediated by lectin-like molecules on the helicobacter binding carbohydrates expressed by host epithelial cells. while clinical isolates of h. pylori do not normally infect mice, some strains have been adapted to colonize this host. we hypothesized that adaptation of h. pylori for colonization of mice may involve alterations in either bacterial ... | 2009 | 19624738 |
| helicobacter pylori infection, host genetics and gastric cancer. | helicobacter pylori infects half the world's population and is responsible for a considerable global health burden, including peptic ulcer disease and gastric cancer. the infection causes a chronic gastritis, the severity and distribution of which determine the clinical outcome. bacterial, environmental and host genetic factors combine to define the degree of gastric damage. most patients have a limited mild pan-gastritis with no significant clinical consequences. antral-predominant gastritis is ... | 2009 | 19659782 |
| the bb genotype of heat-shock protein (hsp) 70-2 gene is associated with gastric pre-malignant condition in h. pylori-infected older patients. | heat-shock protein (hsp) 70 plays essential roles in cellular response to a variety of environmental stresses or unfavorable conditions. a to g transition at the 1267 position of the hsp70-2 gene confers different levels of hsp70 mrna expression. we aimed to clarify the effect of hsp70-2 polymorphism on the risk of premalignant condition, on the degree of acute or chronic inflammation in the stomach. | 2009 | 19661373 |
| role for alpha-l-fucosidase in the control of helicobacter pylori-infected gastric cancer cells. | infecting about one-half of the global human population, helicobacter pylori is well established as the primary cause of gastritis, duodenal ulcer, and gastric cancer. currently there is no clear information regarding if and how host cells interact with h. pylori, and if such interactions are dependent on the type of gastric disease. using fluorescently labeled fucose-containing glycoconjugates, we provide evidence observing both the uptake of l-fucose from gastric cancer cells to h. pylori and ... | 2009 | 19666478 |
| metabolic profiling of helicobacter pylori glycosylation. | metabolic oligosaccharide engineering was used to profile glycoproteins of the human pathogen helicobacter pylori. | 2009 | 19668851 |
| human gastrin: a true growth factor for helicobacter pylori? | 2009 | 19674135 |