Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| analysis of the survival of h. pylori within a laboratory-based aquatic model system using molecular and classical techniques. | despite the significance of helicobacter pylori infection for man, its transmission is not clearly known. the human stomach is considered the reservoir of this pathogen, and one of the accepted routes is fecal-oral, in which water acts as a vector. however, although h. pylori epidemiology associates its transmission with water, only molecular and not cultural analysis detects the bacteria in water. this study was carried out to understand these data through studying the survival of h. pylori in ... | 2007 | 17401696 |
| which has superior acid-suppressive effect, 10 mg omeprazole once daily or 20 mg famotidine twice daily? effects of single or repeated administration in japanese helicobacter pylori-negative cyp2c19 extensive metabolizers. | low-dose omeprazole is superior to full-dose famotidine in maintenance therapy for gastroesophageal reflux disease, whereas "on-demand" famotidine is more effective for relief of episodes of heartburn. to explain this apparent discrepancy, intragastric ph was measured for 24-hr seven times in eight japanese helicobacter pylori-negative cytochrome p450 2c19 extensive metabolizers; on days 1, 8, and 15 of repeated administration of 10 mg of omeprazole once daily and of 20 mg of famotidine twice da ... | 2007 | 17211705 |
| mucin expression in reactive gastropathy: an immunohistochemical analysis. | reactive gastropathy is the second most common diagnosis made on gastric biopsies. increased epithelial proliferation and modifications of epithelial cytokeratin profile, distinct from those of helicobacter pylori gastritis, have been previously reported. however, the evaluation of mucins, important components of the protective mucosal mucous layer, has not been reported. | 2007 | 17227128 |
| epidemiology and pathophysiology of helicobacter pylori infection in children. | helicobacter pylori is one of the commonest bacterial pathogens in human. the organism is associated with development of peptic ulcer diseases, lymphoproliferative disorders and gastric cancer. residence in a developing country, poor socio-economic conditions and genetic predisposition are regarded as risk factors. prevalence of infection is higher in developing countries and re-infection is higher among under five children. it is transmitted mainly through feco-oral route in developing countrie ... | 2007 | 17401270 |
| interaction between the helicobacter pylori caga and alpha-pix in gastric epithelial ags cells. | the gastric pathogen helicobacter pylori (h. pylori) translocates the caga protein into epithelial cells by a type iv secretion process. upon translocation into the host cell cytosol, caga undergoes tyrosine phosphorylation. phosphorylation of caga occurs within the c terminus of the protein and is mediated by members of the src family of tyrosine kinases. phosphorylation of caga induces the dephosphorylation of as yet unidentified cellular proteins, rearrangements of the host cell actin cytoske ... | 2007 | 17405911 |
| expanding the helicobacter pylori genetic toolbox: modification of an endogenous plasmid for use as a transcriptional reporter and complementation vector. | helicobacter pylori is an important human pathogen. however, the study of this organism is often limited by a relative shortage of genetic tools. in an effort to expand the methods available for genetic study, an endogenous h. pylori plasmid was modified for use as a transcriptional reporter and as a complementation vector. this was accomplished by addition of an escherichia coli origin of replication, a kanamycin resistance cassette, a promoterless gfpmut3 gene, and a functional multiple clonin ... | 2007 | 17921278 |
| host response to helicobacter pylori infection before initiation of the adaptive immune response. | helicobacter pylori persistently colonizes the human stomach. in this study, immune responses to h. pylori that occur in the early stages of infection were investigated. within the first 2 days after orogastric infection of mice with h. pylori, there was a transient infiltration of macrophages and neutrophils into the glandular stomach. by day 10 postinfection, the numbers of macrophages and neutrophils decreased to baseline levels. by 3 weeks postinfection, an adaptive immune response was detec ... | 2007 | 17919297 |
| cyp2c19 pharmacogenomics associated with therapy of helicobacter pylori infection and gastro-esophageal reflux diseases with a proton pump inhibitor. | proton pump inhibitors (ppis), such as omeprazole, lansoprazole and rabeprazole, are metabolized by cyp2c19 in the liver. there are genetic differences in the activity of this enzyme. genotypes of cyp2c19 are classified into three groups, rapid metabolizer (rm: *1/*1), intermediate metabolizer (im: *1/*x) and poor metabolizer (pm: *x/*x) (*1 and 'x' represent the wild-type and mutant allele, respectively). the pharmacokinetics and pharmacodynamics of ppis differ among three different cyp2c19 gen ... | 2007 | 17924835 |
| identification, structure and mode of action of a new regulator of the helicobacter pylori hp0525 atpase. | helicobacter pylori is one of the world's most successful human pathogens causing gastric ulcers and cancers. a key virulence factor of h. pylori is the cag pathogenicity island, which encodes a type iv secretion system. hp0525 is an essential component of the cag system and acts as an inner membrane associated atpase. hp0525 forms double hexameric ring structures, with the c-terminal domains (ctds) forming a closed ring and the n-terminal domains (ntds) forming a dynamic, open ring. here, the c ... | 2007 | 17972918 |
| fluorescence diagnostics of helicobacter pylori-infected human gastric mucosa: establishing technique and validity. | bacterial factors, including strain type, anatomic distribution and density, and host responses are important determinants in the pathogenesis of erosive and neoplastic changes linked to gastric helicobacter pylori (h. pylori) infection. the purpose of this study was to investigate the potential use of photodiagnostics in mapping h. pylori infection. the relationship between fluorescence in individual gastric pits of h. pylori(+) and h. pylori(-) subjects versus that in larger field views of the ... | 2007 | 17613923 |
| interaction of muc1 with beta-catenin modulates the wnt target gene cyclind1 in h. pylori-induced gastric cancer. | beta-catenin can function as an oncogene when it is translocated to the nucleus, binds to t-cell factor (tcf) or lymphoid enhance factor and transactivate its target gene. the mechanism responsible for the activation of wnt signaling pathway in the cytotoxin-associated antigen a (caga) helicobacter pylori (h. pylori)-infected gastric carcinoma has not been elucidated. we hypothesize that whether interaction of muc1 with beta-catenin modulates the wnt signaling and its target gene cyclind1 in cag ... | 2007 | 17393422 |
| flavodoxin:quinone reductase (fqrb): a redox partner of pyruvate:ferredoxin oxidoreductase that reversibly couples pyruvate oxidation to nadph production in helicobacter pylori and campylobacter jejuni. | pyruvate-dependent reduction of nadp has been demonstrated in cell extracts of the human gastric pathogen helicobacter pylori. however, nadp is not a substrate of purified pyruvate:ferredoxin oxidoreductase (pfor), suggesting that other redox active enzymes mediate this reaction. here we show that fqrb (hp1164), which is essential and highly conserved among the epsilonproteobacteria, exhibits nadph oxidoreductase activity. fqrb was purified by nickel interaction chromatography following overexpr ... | 2007 | 17468253 |
| a search engine to identify pathway genes from expression data on multiple organisms. | the completion of several genome projects showed that most genes have not yet been characterized, especially in multicellular organisms. although most genes have unknown functions, a large collection of data is available describing their transcriptional activities under many different experimental conditions. in many cases, the coregulatation of a set of genes across a set of conditions can be used to infer roles for genes of unknown function. | 2007 | 17477880 |
| enhanced activation of cyclooxygenase-2 downregulates th1 signaling pathway in helicobacter pylori-infected human gastric mucosa. | evidence suggests that an impaired t-cell response against helicobacter pylori plays a role in the pathogenesis of h. pylori-related diseases. cyclooxygenase (cox) 2 has been shown to inhibit the production of t-helper (th) 1 cytokines. this study aimed to ascertain whether cox-2 downregulates th1 signaling pathway in human gastric mucosa colonized by h. pylori. | 2007 | 17492998 |
| a proteomic approach for the identification of bismuth-binding proteins in helicobacter pylori. | helicobacter pylori is a major human pathogen that can cause peptic ulcers and chronic gastritis. bismuth-based triple or quadruple therapies are commonly recommended for the treatment of h. pylori infections. however, the molecular mechanisms underlying treatment with bismuth are currently not fully understood. we have conducted a detailed comparative proteomic analysis of h. pylori cells both before and after treatment with colloidal bismuth subcitrate (cbs). eight proteins were found to be si ... | 2007 | 17503094 |
| helicobacter pylori caga targets par1/mark kinase to disrupt epithelial cell polarity. | helicobacter pylori caga-positive strains are associated with gastritis, ulcerations and gastric adenocarcinoma. caga is delivered into gastric epithelial cells and, on tyrosine phosphorylation, specifically binds and activates the shp2 oncoprotein, thereby inducing the formation of an elongated cell shape known as the 'hummingbird' phenotype. in polarized epithelial cells, caga also disrupts the tight junction and causes loss of apical-basolateral polarity. we show here that h. pylori caga spec ... | 2007 | 17507984 |
| [carbamazepine and clarithromycin: a clinically relevant drug interaction]. | carbamazepine is associated with clinically relevant drug interactions especially with macrolide antibiotics such as troleandomycin and erythromycin. these drugs inhibit the metabolism of carbamazepine. clarithromycin, a macrolide antibiotic similar to erythromycin, is widely used to treat respiratory tract infections and is used for the treatment of atypical mycobacterial infections and helicobacter pylori-associated peptic ulcer disease. | 2007 | 18033049 |
| re-expression of sonic hedgehog and reduction of cdx2 after helicobacter pylori eradication prior to incomplete intestinal metaplasia. | loss of sonic hedgehog (shh) and aberrant cdx2 expression are early changes correlating with the presence of intestinal metaplasia that occur in the gastric mucosa prior to neoplastic transformation. the aim of this study was to compare the improvement in corpus gastritis with shh and cdx2 expression after h. pylori eradication between subjects at high risk for gastric cancer and controls. the usefulness of serum pepsinogen levels as a predictor of resolved corpus gastritis was also examined. se ... | 2007 | 17520681 |
| microbial genomes to write our history. | 2007 | 17624832 | |
| isolation and characterization of human gastric cell lines with stem cell phenotypes. | the aim of this study was to develop an in vitro human gastric stem and/or progenitor cell model that may be used to study the mechanism of gastric carcinogenesis induced by helicobacter pylori infection. | 2007 | 17645461 |
| the language used by helicobacter pylori to regulate human cells. | 2007 | 17538876 | |
| prognostic significance of maspin expression in human gastric adenocarcinoma. | maspin is a member of the serpin family of protease inhibitors known to have tumor suppressor activity. however, molecular aspects in carcinogenesis and progression of gastric cancer remain largely unclear. previously we reported for the first time that maspin is induced in the course of gastric carcinogenesis. in the present study we evaluated maspin induction in gastric adenocarcinoma in relation to a number of clinicopathologic feature. | 2007 | 17591106 |
| improved in vitro model systems for gastrointestinal infection by choice of cell line, ph, microaerobic conditions, and optimization of culture conditions. | commonly used in vitro infection cultures do not mimic the human gastrointestinal tract with regard to ph and microaerobic conditions. furthermore, despite the importance of mucin-helicobacter interactions, the cell lines used have not been selected for appropriate mucin expression. to make in vitro studies more applicable to human disease, we have developed coculture methods taking these factors into account. | 2007 | 17669108 |
| expression of helicobacter pylori virulence factors and associated expression profiles of inflammatory genes in the human gastric mucosa. | helicobacter pylori virulence factors have been suggested to be important in determining the outcome of infection. the h. pylori adhesion protein baba2 is thought to play a crucial role in bacterial colonization and in induction of severe gastric inflammation, particularly in combination with expression of caga and vaca. however, the influence of these virulence factors on the pathogenesis of h. pylori infection is still poorly understood. to address this question, the inflammatory gene expressi ... | 2007 | 17709414 |
| helicobacter pylori in the palatine tonsils of patients with iga nephropathy compared with those of patients with recurrent pharyngotonsillitis. | helicobacter pylori infection is acquired by oral ingestion. however, the morphology and microscopic localization of h pylori in the human oral cavity and pharynx are unknown. in the present study, we performed immunohistochemistry, immunoelectron microscopy, in situ hybridization, and polymerase chain reaction to identify h pylori in the palatine tonsils of 32 patients with immunoglobulin a nephropathy (igan) and 141 patients with recurrent pharyngotonsillitis (rpt). h pylori in coccoid form wa ... | 2007 | 17714758 |
| clinical impact of genetic aberrations in gastric malt lymphoma: a comprehensive analysis using interphase fluorescence in situ hybridisation. | there is a need for genetic biomarkers to guide prognosis and management of gastric mucosa-associated lymphoid tissue (malt) lymphomas. we assessed the incidence and clinical significance of the malt lymphoma-associated genetic abnormalities t(11;18)/api2-malt1, t(1;14)/bcl10-igh, t(14;18)/igh-malt1, t(3;14)/foxp1-igh, and extra copies of malt1 and foxp1 in gastric malt lymphomas from japan. | 2007 | 17525089 |
| overexpression of helicobacter pylori-associated urease mrnas in human gastric cancer. | urease is involved in helicobacter pylori infection and survival in acid circumference. this study explored the overexpression of h. pylori-associated urease mrnas in human gastric cancers by using a well-established membrane array analysis method in our lab. analysis of 30 gastric cancer tissue specimens and 30 paired adjacent normal tissues demonstrated that urease genes involved in h. pylori infection were upregulated in gastric cancer tissues. urea, g, and i are predominant genotypes found i ... | 2007 | 17716001 |
| helicobacter pylori induces ccl20 expression. | ccl20 attracts immature dendritic cells and memory t cells and plays a role on mucosal surfaces in inflammation. however, whether helicobacter pylori infection induces ccl20 in human gastric epithelial cells remains to be determined. the aim of this study was to analyze the molecular mechanism of h. pylori-induced ccl20 expression. expression of ccl20 mrna was assessed by reverse transcription-pcr. five normal and five h. pylori-infected gastric tissue samples were stained immunohistochemically ... | 2007 | 17724069 |
| association of myeloperoxidase -463 g/a polymorphism with clinical outcome of helicobacter pylori infection in iranian patients with gastrointestinal diseases. | polymorphisms in the immune related genes are important in the clinical outcome of helicobacter pylori infection. myeloperoxidase -463 g/a polymorphism has been shown to reduce enzyme expression and activity. | 2007 | 17767014 |
| primary gastrointestinal lymphoma: spectrum of imaging findings with pathologic correlation. | gastrointestinal lymphoma is an uncommon disease but is the most frequently occurring extranodal lymphoma and is almost exclusively of non-hodgkin type. primary gastrointestinal lymphoma most commonly involves the stomach but can involve any part of the gastrointestinal tract from the esophagus to the rectum. risk factors for the development of gastrointestinal lymphoma include helicobacter pylori infection, immunosuppression after solid organ transplantation, celiac disease, inflammatory bowel ... | 2007 | 17848697 |
| muc1 mucin limits both helicobacter pylori colonization of the murine gastric mucosa and associated gastritis. | the muc1 mucin is expressed on the cell surface of epithelial cells lining the gastric mucosa. epidemiologic studies suggest that functional allelic variations in the muc1 gene may play a role in human susceptibility to helicobacter pylori-associated pathologies, including gastric adenocarcinoma. we have evaluated the impact of muc1 expression on the colonization and pathogenesis of gastric helicobacter infections. | 2007 | 17919495 |
| role of a muty dna glycosylase in combating oxidative dna damage in helicobacter pylori. | muty is an adenine glycosylase that has the ability to efficiently remove adenines from adenine/7,8-dihydro-8-oxoguanine (8-oxo-g) or adenine/guanine mismatches, and plays an important role in oxidative dna damage repair. the human gastric pathogen helicobacter pylori has a homolog of the muty enzyme. to investigate the physiological roles of muty in h. pylori, we constructed and characterized a muty mutant. h. pylori muty mutants incubated at 5% o2 have a 325-fold higher spontaneous mutation ra ... | 2007 | 16996809 |
| expression, purification and characterization of the membrane-associated hrca repressor protein of helicobacter pylori. | helicobacter pylori, a microaerophilic, gram-negative bacterium is a human pathogen that colonizes the gastric niche and is associated with several acute and chronic stomach diseases. in order to survive in the gastric environment and become pathogenic, the bacterium relies on a plethora of virulence factors, which also include heat shock proteins. we previously showed that two out of the three operons encoding the major cellular chaperone machineries are transcriptionally repressed by two regul ... | 2007 | 16997572 |
| interplay between helicobacter pylori and host gene polymorphisms in inducing oxidative dna damage in the gastric mucosa. | infection by helicobacter pylori is the most important risk factor for gastric cancer. however, only a small fraction of colonized individuals, representing at least half of the world's population, develop this malignancy. in order to shed light on host-microbial interactions, gastric mucosa biopsies were collected from 119 patients suffering from dyspeptic symptoms. 8-hydroxy-2'-deoxyguanosine (8-oxo-dg) levels in the gastric mucosa were increased in carriers of h.pylori, detected either by cul ... | 2007 | 17127715 |
| effects of vaccination by a recombinant antigen ureb138 (a segment of the beta-subunit of urease) against helicobacter pylori infection. | helicobacter pylori has to counteract acidity during colonization in the stomach. the most important region for the enzymic activity of h. pylori urease, consisting of 138 aa (ureb138), was determined by a comparison of the homology of amino acid sequences, and a structural analysis, between urease of h. pylori and various other species. this region was expressed in escherichia coli as a fusion protein with glutathione s-transferase (gst), which was cleaved by prescission protease between the gs ... | 2007 | 17510273 |
| serological assays for identification of human gastric colonization by helicobacter pylori strains expressing vaca m1 or m2. | the helicobacter pylori vaca gene encodes a secreted protein (vaca) that alters the function of gastric epithelial cells and t lymphocytes. h. pylori strains containing particular vaca alleles are associated with differential risk of disease. because the vaca midregion may exist as one of two major types, m1 or m2, serologic responses may potentially be used to differentiate between patients colonized with vaca m1- or vaca m2-positive h. pylori strains. in this study, we examined the utility of ... | 2007 | 17267587 |
| the relationship between helicobacter pylori infection and promoter polymorphism of the nrf2 gene in chronic gastritis. | the transcription factor nrf2 regulates the expression of detoxifying and antioxidant genes. three polymorphisms of the nrf2 gene have been reported. we attempted to clarify the relationship between nrf2 gene polymorphism and chronic gastritis in a japanese population. the study was performed in 159 patients with no evidence of gastric malignancy on upper gastrointestinal endoscopy (mean age, 62.03 years; male:female ratio, 102:57; peptic ulcer diseases in 69 patients, and helicobacter pylori (h ... | 2007 | 17143558 |
| cytochrome p450 2c19 polymorphism influences the preventive effect of lansoprazole on the recurrence of erosive reflux esophagitis. | the efficacy of lansoprazole (lpz) at inhibiting gastric acid secretion is influenced by cytochrome p450 2c19 (cyp2c19) polymorphism. the purpose of the present study was to investigate whether cyp2c19 polymorphism had an influence on the remission of erosive reflux esophagitis (re) during maintenance therapy with lpz. | 2007 | 17295875 |
| cytokine expression due to helicobacter pylori in a tissue culture model. | helicobacter pylori, in recent years, has been recognized as the major causative agent in chronic gastritis and peptic ulcer disease in humans. h. pylori is a ubiquitous organism, with at least half of the world's population infected. of those individuals with peptic ulcer disease, it is estimated that 90% of cases are caused by h. pylori. currently, the efficacy of therapies is starting to decline due to increasing resistance rates, especially towards clarithromycin. due to this, new therapies ... | 2007 | 17160356 |
| variant size- and glycoforms of the scavenger receptor cysteine-rich protein gp-340 with differential bacterial aggregation. | glycoprotein gp-340 aggregates bacteria in saliva as part of innate defence at mucosal surfaces. we have detected size- and glycoforms of gp-340 between human saliva samples (n = 7) and lung gp-340 from a proteinosis patient using antibodies and lectins in western blots and elisa measurements. western blots of saliva samples, and of gp-340 purified, from the seven donors using a gp-340 specific antibody distinguished four gp-340 size variants, designated i to iv (n = 2,2,2 and 1). while saliva g ... | 2007 | 17243023 |
| defensins and cathelicidins in gastrointestinal infections. | to review recently published studies presenting novel and relevant information on antimicrobial peptides in gastrointestinal infections. | 2007 | 17133082 |
| quantitative determination of gland mucous cells-type mucin using a monoclonal antibody, hik1083: its pathophysiological changes in human gastric juice. | pathological alteration in gastric mucosa is caused by helicobacter pylori infection and is detectable by histological analysis. in particular, the alteration of gland mucous cells (gmcs)-type mucin, which plays a protective role against h. pylori infection, is critical in the pathogenesis of h. pylori-related gastritis. we established an assay for gmcs-type mucin and quantitatively assessed the pathophysiological changes in its content in human gastric juice samples. | 2007 | 17134689 |
| allergic sensitization and microbial load--a comparison between finland and russian karelia. | epidemiological data have indicated that some infections are associated with a low risk of allergic diseases, thus supporting the idea (hygiene hypothesis) that the microbial load is an important environmental factor conferring protection against the development of allergies. we set out to test the hygiene hypothesis in a unique epidemiological setting in two socio-economically and culturally markedly different, although genetically related, populations living in geographically adjacent areas. t ... | 2007 | 17302731 |
| [gastric relapse of stage i ocular adnexal mucosa-associated lymphoid tissue lymphoma]. | a 57-year-old male with right ocular adnexal mucosa-associated lymphoid tissue lymphoma (malt lymphoma) was successfully treated with local radiation therapy. the gastroendoscopic examination revealed a slight inflammatory change of the gastric mucosa, and the urease test was positive. eradication therapy against helicobacter pylori was successfully done, however, the patient developed gastric malt lymphoma two years after the initial treatment. southern blot analysis of the immunoglobulin heavy ... | 2007 | 17313077 |
| helicobacter pylori stimulates urokinase plasminogen activator receptor expression and cell invasiveness through reactive oxygen species and nf-kappab signaling in human gastric carcinoma cells. | the gastric pathogen, helicobacter pylori (h. pylori), has been associated with the progression of gastric cancer. it was previously reported that h. pylori induced urokinase plasminogen activator receptor (upar) expression and stimulated cell invasiveness in human gastric cancer ags cells. however, the precise mechanisms for how h. pylori upregulates upar are unclear. this study investigated the underlying signal pathways in h. pylori-induced upar in human gastric cancer ags cells. the intracel ... | 2007 | 17334646 |
| effect of mdr1 c3435t polymorphism on cure rates of helicobacter pylori infection by triple therapy with lansoprazole, amoxicillin and clarithromycin in relation to cyp 2c19 genotypes and 23s rrna genotypes of h. pylori. | polymorphism in mdr1 is associated with variation in the plasma level of a proton pump inhibitor. | 2007 | 17697203 |
| human gastric glycosphingolipids recognized by helicobacter pylori vacuolating cytotoxin vaca. | many bacterial toxins utilize cell surface glycoconjugate receptors for attachment to target cells. in the present study the potential carbohydrate binding of helicobacter pylori vacuolating cytotoxin vaca was investigated by binding to human gastric glycosphingolipids on thin-layer chromatograms. thereby a distinct binding of the toxin to two compounds in the non-acid glycosphingolipid fraction was detected. the vaca-binding glycosphingolipids were isolated and characterized by mass spectrometr ... | 2007 | 17400502 |
| genetic susceptibility to nonsteroidal anti-inflammatory drug-related gastroduodenal bleeding: role of cytochrome p450 2c9 polymorphisms. | several nonsteroidal anti-inflammatory drugs (nsaids) are metabolized by the cytochrome p450 2c9 (cyp2c9). two common variants of the cyp2c9 gene (cyp2c9*2 and *3) were reported to significantly affect the activity of the cyp2c9 enzyme. the aim of this study was to evaluate the impact of cyp2c9 polymorphisms on the risk of gastroduodenal bleeding in acute nsaid users. | 2007 | 17681167 |
| [antibiotic resistance of helicobacter pylori]. | to examine the infection and bacteria resistance of helicobacter pylori (h.pylori) to clarithromycin and furazolidone,to determine whether the antibiotic resistance is primary or secondary, and to decide if a new h.pylori infection plays a role in eradication failures. | 2007 | 17611323 |
| celecoxib inhibits cdx2 expression and prevents gastric cancer in helicobacter pylori-infected mongolian gerbils. | the aim of this study was to see whether administration of celecoxib, a selective cox-2 inhibitor, prior to the appearance of intestinal metaplasia could prevent the development of gastric cancer in helicobacter pylori-infected mongolian gerbils. | 2007 | 17341852 |
| no association between helicobacter pylori seropositivity and ornithine decarboxylase (odc) a317g polymorphism, and no modification by nad(p)h:qinone oxidoreductase 1 (nqo1) c609t. | our previous epidemiologic study reported that nad(p)h:quinone oxidoreductase 1 (nqo1) 609c/c with full enzyme activity was a high risk genotype for helicobacter pylori (h. pylori) seropositivity. since nqo1 stabilizes ornithine decarboxylase (odc), which attenuates the innate immune response through elevated polyamines, odc functional polymorphisms may also influence h. pylori seropositivity. this study aimed to examine the association with odc a317g polymorphism, as well as the modification by ... | 2007 | 17378176 |
| clarithromycin resistance, tumor necrosis factor alpha gene polymorphism and mucosal inflammation affect h. pylori eradication success. | several bacterial and host-related factors concur in causing helicobacter pylori eradication failure. we ascertained the role of bacterial virulence genes (caga, vaca), clarithromycin resistance [cla(r), 23s ribosomal rna (rrna) mutations], host polymorphism of cyp2c19 (polyphosphoinositide, ppi, metabolism) and of the cytokines il-1b-31c>t, il-1rn vntr, ifn-gamma+874a>t, tnf-alpha-1031t>c, tnf-alpha-857c>t, tnf-alpha-376g>a, tnf-alpha-308g>a, tnf-alpha-238g>a, il-10-1082a>g, il-10-819c>t, il-10 ... | 2007 | 17846855 |
| helicobacter pylori-negative low-grade extranodal b-cell primary gastric mucosa-associated lymphoid tissue lymphoma. | helicobacter pylori-negative primary mucosa-associated lymphoid tissue (malt) lymphoma of the stomach--a primary gastric lymphoma (pgl)--is not very common. herein, we report a 77-year-old man with the chief complaint of intermittent epigastric pain. initial upper gastrointestinal (ugi) endoscopy showed multiple irregular ulcers (gus) over the antrum with poor distention of the stomach, and gastric adenocarcinoma (borrmann type iv, linitis plastica) was suspected. but the biopsy specimens of the ... | 2007 | 17389157 |
| activation of human neutrophils with helicobacter pylori and the role of toll-like receptors 2 and 4 in the response. | the innate immune response to helicobacter pylori infection is beginning to be understood and recent works support a role for toll-like receptors (tlrs). our aim was to study the response of human neutrophils to h. pylori and to elucidate the role of tlr2 and tlr4. neutrophils from healthy h. pylori-negative volunteers were cocultured with h. pylori 26695 strain. the release of il-8, il-1beta, tumor necrosis factor (tnf)-alpha and il-10 was measured. the role of tlr2 and tlr4 was investigated wi ... | 2007 | 17892476 |
| [construction, expression and purification of ureb-omp11 fusion protein of helicobacter pylori and its immunocompetence]. | to construct h.pylori vaccine candidate strain expressing ureb-omp11 recombinant fusion protein of h.pylori. to express and purify the fusion protein ureb-omp11 and to determine its immunocompetence. | 2007 | 17908495 |
| helicobacter pylori caga induces ags cell elongation through a cell retraction defect that is independent of cdc42, rac1, and arp2/3. | helicobacter pylori, which infects over one-half the world's population, is a significant risk factor in a spectrum of gastric diseases, including peptic ulcers and gastric cancer. strains of h. pylori that deliver the effector molecule caga into host cells via a type iv secretion system are associated with more severe disease outcomes. in a tissue culture model of infection, caga delivery results in a dramatic cellular elongation referred to as the "hummingbird" phenotype, which is characterize ... | 2007 | 17194805 |
| participation of capsaicin-sensitive afferent nerves in the gastric mucosa of patients with helicobacter pylori-positive or-negative chronic gastritis. | capsaicin-sensitive afferent nerves (csans) are involved in the protection of gastric mucosa. to clarify the role of csans in human helicobacter pylori-negative or -positive chronic gastritis, after bacterium detection by rapid urease test, (14)c urea breath test, and specific histological staining, the immunodistribution of capsaicin receptor, calcitonin gene-related peptide (cgrp), and substance p (sp) was studied in 21 h. pylori-positive and 30 h. pylori-negative patients with chronic gastrit ... | 2007 | 17195120 |
| antisense rna modulation of alkyl hydroperoxide reductase levels in helicobacter pylori correlates with organic peroxide toxicity but not infectivity. | much of the gene content of the human gastric pathogen helicobacter pylori ( approximately 1.7-mb genome) is considered essential. this view is based on the completeness of metabolic pathways, infrequency of nutritional auxotrophies, and paucity of pathway redundancies typically found in bacteria with larger genomes. thus, genetic analysis of gene function is often hampered by lethality. in the absence of controllable promoters, often used to titrate gene function, we investigated the feasibilit ... | 2007 | 17337572 |
| resistance of primary murine cd4+ t cells to helicobacter pylori vacuolating cytotoxin. | persistent colonization of the human stomach by helicobacter pylori is a risk factor for the development of gastric cancer and peptic ulcer disease. h. pylori secretes a toxin, vaca, that targets human gastric epithelial cells and t lymphocytes and enhances the ability of h. pylori to colonize the stomach in a mouse model. to examine how vaca contributes to h. pylori colonization of the mouse stomach, we investigated whether murine t lymphocytes were susceptible to vaca activity. vaca inhibited ... | 2007 | 17074854 |
| construction of recombinant attenuated salmonella typhimurium dna vaccine expressing h pylori ureb and il-2. | to construct a recombinant live attenuated salmonella typhimurium dna vaccine encoding h pylori ureb gene and mouse il-2 gene and to detect its immunogenicity in vitro and in vivo. | 2007 | 17352028 |
| polymorphisms in th1-type cell-mediated response genes and risk of gastric cancer. | helicobacter pylori infection, the dominant risk factor for gastric cancers, has been shown to elicit t helper type 1 (th1) polarized immunological responses. we conducted a population-based study of 305 gastric cancer cases and 427 age- and gender-matched controls in warsaw, poland, to evaluate the association with several variants in genes responsible for th1-cell-mediated response. genotyping was performed on genomic dna by taqman(tm) assays to determine tnfa (-308 g>a, -417 g>a, -555 g>a, -1 ... | 2007 | 16885196 |
| eradication of helicobacter pylori might halt the progress to oesophageal adenocarcinoma in patients with gastro-oesophageal reflux disease and barrett's oesophagus. | 2007 | 17196756 | |
| identification of helicobacter pylori genes that contribute to stomach colonization. | chronic infection of the human stomach by helicobacter pylori leads to a variety of pathological sequelae, including peptic ulcer and gastric cancer, resulting in significant human morbidity and mortality. several genes have been implicated in disease related to h. pylori infection, including the vacuolating cytotoxin and the cag pathogenicity island. other factors important for the establishment and maintenance of infection include urease enzyme production, motility, iron uptake, and stress res ... | 2007 | 17101654 |
| dynamic colonization of helicobacter pylori in human gastric mucosa. | to investigate the dynamic behaviour of helicobacter pylori in the colonization of the human gastric mucosa in patients previously treated for h. pylori infection. | 2008 | 17918004 |
| toll-like receptor 2 (tlr) -196 to 174del polymorphism in gastro-duodenal diseases in japanese population. | toll-like receptors (tlrs) play important roles in the signaling of many pathogen-related molecules and endogenous proteins associated with immune activation. -196 to -174del polymorphism affects the tlr2 gene and alters its promoter activity. we investigated the influence of tlr2 -196 to -174del polymorphism on the risk of gastro-duodenal diseases, on the severity of helicobacter pylori-induced gastritis in a japanese population. the study was performed on 309 patients with abdominal discomfort ... | 2008 | 17934843 |
| enzymatic characterization and inhibitor discovery of a new cystathionine {gamma}-synthase from helicobacter pylori. | cystathionine gamma-synthase (cgs) catalyses the first step of the transsulfuration pathway that converts l-cysteine to l-homocysteine in bacteria, whereas this pathway is absent in human. in this report, we identified a new metb gene from helicobacter pylori strain ss1, and the recombinant h. pylori cystathionine gamma-synthase (hpcgs) was successfully cloned, expressed and purified in escherichia coli system. enzymatic study of hpcgs indicated that the k(m) and k(cat)/k(m) values against the s ... | 2008 | 17981822 |
| autoantibodies against appetite-regulating peptide hormones and neuropeptides: putative modulation by gut microflora. | peptide hormones synthesized in gastrointestinal and adipose tissues in addition to neuropeptides regulate appetite and body weight. previously, autoantibodies directed against melanocortin peptides were found in patients with eating disorders; however, it remains unknown whether autoantibodies directed against other appetite-regulating peptides are present in human sera and whether their levels are influenced by gut-related antigens. | 2008 | 18262391 |
| vitamin c supplementation does not protect l-gulono-gamma-lactone oxidase-deficient mice from helicobacter pylori-induced gastritis and gastric premalignancy. | in human studies, low vitamin c intake has been associated with more severe helicobacter pylori gastritis and a higher incidence of gastric cancer. however, vitamin c supplementation has not been definitively shown to protect against gastric cancer. using vitamin c-deficient b6.129p2-gulo(tm1umc/mmcd) (gulo(-/-)) mice lacking l-gulono-gamma-lactone oxidase, we compared gastric lesions and th1 immune responses in h. pylori-infected gulo(-/-) mice supplemented with low (33 mg/l) or high (3,300 mg/ ... | 2008 | 17990318 |
| altered gastric corpus expression of tissue inhibitors of metalloproteinases in human and murine helicobacter infection. | matrix metalloproteinases (mmps) have roles in inflammation and other processes relevant to the architectural disturbances seen in the gastric mucosa in response to helicobacter pylori infection. upregulation of mmps has been reported in h pylori infection, but there are no detailed reports regarding altered production of their inhibitors, the tissue inhibitors of metalloproteinases (timps). | 2008 | 17412866 |
| role of type iv secretion in helicobacter pylori pathogenesis. | helicobacter pylori is a highly successful human-specific gastric pathogen that colonizes more than half the world's population. infection with this bacterium can induce gastric pathologies ranging from chronic gastritis to peptic ulcers and even cancer. virulent h. pylori isolates harbour the cag (cytotoxin-associated genes) pathogenicity island, a 40 kb stretch of dna that encodes components of a type iv secretion system (t4ss). this t4ss forms a pilus for the injection of virulence factors in ... | 2008 | 18410539 |
| [proteomic analysis of helicobacter pylori in human gastritis and gastric cancer]. | to establish 2-dimensional electrophoresis (2-de) maps of helicobac-ter pylori in human gastritis, and gastric cancer, to identify the differentially expressed proteins,and to discuss the role of bacterial factor in pathogenesis. | 2008 | 18544840 |
| quadruple rescue therapy for helicobacter pylori infection after two treatment failures. | a standard third-line therapy for helicobacter pylori infection is lacking, and antimicrobial sensitivity data for patients who failed eradication therapy are often unavailable in clinical practice. we therefore designed the prospective study to assess the efficacy of levofloxacin, amoxicillin, bismuth and rabeprazole quadruple therapy as a third-line treatment for h. pylori infection. | 2008 | 18435764 |
| the alpha and beta classes carbonic anhydrases from helicobacter pylori as novel drug targets. | helicobacter pylori (h. pylori) successfully resides in the human stomach in highly acidic conditions, causing a variety of gastroduodenal lesions, including gastric ulcer, gastric cancer and malt lymphoma. for acid acclimation of h. pylori, two types of enzymes, urease and carbonic anhydrase (ca), play a central role. they cooperatively function to maintain neutral ph in the bacterial cytoplasm and periplasm. the genome project of h. pylori identified two different classes of ca with different ... | 2008 | 18336307 |
| the role of infectious agents in the etiology of ocular adnexal neoplasia. | given the fact that infectious agents contribute to around 18% of human cancers worldwide, it would seem prudent to explore their role in neoplasms of the ocular adnexa: primary malignancies of the conjunctiva, lacrimal glands, eyelids, and orbit. by elucidating the mechanisms by which infectious agents contribute to oncogenesis, the management, treatment, and prevention of these neoplasms may one day parallel what is already in place for cancers such as cervical cancer, hepatocellular carcinoma ... | 2008 | 18572051 |
| h pylori (caga) and epstein-barr virus infection in gastric carcinomas: correlation with p53 mutation and c-myc, bcl-2 and bax expression. | to investigate the interrelationship between h pylori and epstein-barr virus (ebv) infection in the gastric carcinogenesis having in focus the p53 mutation and the c-myc, bcl-2 and bax expression. | 2008 | 18240345 |
| yeast of the oral cavity is the reservoir of heliobacter pylori. | frequent occurrence of helicobacter pylori in the human gastrointestinal tract and its persistence due to unsuccessful antimicrobial therapy might be related to a stage in the life cycle of h. pylori in which the bacterium establishes itself as an intracellular symbiont in yeast. in this study, occurrence of non-culturable h. pylori in the oral yeast was assessed by targeting vacuolating cytotoxin a (vaca s1s2) and ureab genes in the total dnas of yeasts. | 2008 | 18266659 |
| difference of p53aip1 mrna expression in gastric mucosa between patients with gastric cancer and chronic gastritis infected with helicobacter pylori. | to examine the significance of alteration of p53-regulated apoptosis-induced protein 1 (p53aip1) expression in gastric carcinogenesis in patients with helicobacter pylori infection. | 2008 | 18277906 |
| identification of proteins related to nickel homeostasis in helicobater pylori by immobilized metal affinity chromatography and two-dimensional gel electrophoresis. | helicobacter pylori (h. pylori) is a widespread human pathogen causing peptic ulcers and chronic gastritis. maintaining nickel homeostasis is crucial for the establishment of h. pylori infection in humans. we used immobilized-nickel affinity chromatography to isolate ni-related proteins from h. pylori cell extracts. two-dimensional gel electrophoresis and mass spectrometry were employed to separate and identify twenty two ni-interacting proteins in h. pylori. these ni-interacting proteins can be ... | 2008 | 18288244 |
| pseudomyxoma peritonei: is disease progression related to microbial agents? a study of bacteria, muc2 and muc5ac expression in disseminated peritoneal adenomucinosis and peritoneal mucinous carcinomatosis. | pseudomyxoma peritonei (pmp) is characterized by peritoneal tumors arising from a perforated appendiceal adenoma or adenocarcinoma, but associated entry of enteric bacteria in the peritoneum has not been considered as a cofactor. because gram-negative organisms can upregulate muc2 mucin gene expression, we determined whether bacteria were detectable in pmp tissues. | 2008 | 18299935 |
| effect of eradication of helicobacter pylori on the histology and cellular phenotype of gastric intestinal metaplasia. | eradication of helicobacter pylori appears to reduce gastric cancer incidence. we examined the effect of successful h pylori therapy on histology, phenotype of gastric intestinal metaplasia (gim) (complete vs incomplete), and expression of several biomarkers related to carcinogenesis. | 2008 | 18321787 |
| cdh1 gene polymorphisms, smoking, helicobacter pylori infection and the risk of gastric cancer in the european prospective investigation into cancer and nutrition (epic-eurgast). | despite declining incidence rates, gastric cancer (gc) is a major cause of death worldwide. e-cadherin is an adhesion molecule that is thought to be involved in gc. germline mutations in the e-cadherin gene (cdh1) have been identified in hereditary diffuse gc. also, a promoter polymorphism at position -160 c/a has been suggested to lead to transcriptional down regulation and has been shown to affect gc risk in some studies. however, very little information exists on the gc risk association of ot ... | 2008 | 18342503 |
| chronic autoimmune thyroiditis (hashimoto's thyroiditis) in patients with malt lymphoma. | autoimmune diseases have been implicated in the genesis of malt lymphoma of various localizations. the development of thyroidal malt lymphoma has been described as an adverse event in patients suffering from long-standing chronic autoimmune thyroiditis (cat, hashimoto's thyroiditis). the percentage and possible association between cat and extrathyroidal malt lymphoma, however, have not been assessed so far. | 2008 | 18334510 |
| significantly elevated helicobacter pylori density and different genotype distribution in erosions as compared with normal gastric biopsy specimen detected by quantitative real-time pcr. | determination of the local densities of helicobacter pylori and its genotypic variations in gastric biopsy specimens by using novel real-time pcr-based methods could support the precise diagnosis and understanding of h. pylori infections. | 2008 | 18334874 |
| the distribution of 4-hydroxynonenal-modified proteins in gastric mucosa of duodenal peptic ulcer patients. | this study used monoclonal antibody specific for 4-hydroxynonenal (hne)-histidine to evaluate immunohistochemical distribution of hne-protein adducts in gastric mucosa biopsies of 52 peptic ulcer patients (all positive for h. pylori) and of 20 healthy volunteers (eight positive and 12 negative for h. pylori). hne-modified proteins were present in glandular epithelium in all subjects, both patients with duodenal peptic ulcer and healthy subjects. hence, the presence of hne did not appear to be re ... | 2008 | 18344114 |
| cyp2c19 genotypes and helicobacter pylori eradication. | 2008 | 18699976 | |
| polymorphisms of death pathway genes fas and fasl and risk of premalignant gastric lesions. | tumorigenesis is a multistep process that begins with the abrogation of normal controls of apoptosis and cell proliferation, and the fas receptor-ligand system is a key regulator of apoptosis. the aim of this study was to investigate whether functional polymorphisms of death pathway genes (fas and fasl) are associated with the development of gastric atrophy and intestinal metaplasia. | 2008 | 18383830 |
| tnf-alpha-inducing protein, a carcinogenic factor secreted from h. pylori, enters gastric cancer cells. | tnf-alpha inducing protein (tip alpha) is secreted from helicobacter pylori (h. pylori): it is a potent inducer of tnf-alpha and chemokine genes, mediated through nf-kappab activation, and it also induces tumor-promoting activity in bhas 42 cells. to investigate the carcinogenic mechanisms of h. pylori with tip alpha, we first examined how tip alpha acts on gastric epithelial cells. we found that fluorescent-tip alpha specifically bound to, and then entered, the cells in a dose- and temperature- ... | 2008 | 18412243 |
| hancornia speciosa: indications of gastroprotective, healing and anti-helicobacter pylori actions. | mangaba (hancornia speciosa gomez) is a medicinal plant frequently cited in ethnopharmacological inventories of the central region of brazil against gastrointestinal disorders such as diarrhoea, ulcer, gastritis and stomach ache. | 2008 | 18761076 |
| transgenic expression of helicobacter pylori caga induces gastrointestinal and hematopoietic neoplasms in mouse. | infection with caga-positive helicobacter pylori is associated with gastric adenocarcinoma and gastric mucosa-associated lymphoid tissue (malt) lymphoma of b cell origin. the caga-encoded caga protein is delivered into gastric epithelial cells via the bacterial type iv secretion system and, upon tyrosine phosphorylation by src family kinases, specifically binds to and aberrantly activates shp-2 tyrosine phosphatase, a bona fide oncoprotein in human malignancies. caga also elicits junctional and ... | 2008 | 18192401 |
| polymorphisms in toll-like receptor genes and risk of cancer. | host genetic factors are emerging as key determinants of disease risk for many cancers. identifying candidate genes is a major challenge that has to stem from a profound understanding of the pathophysiology of the disease. the toll-like receptors are important members of the host's innate immune response and their genes have been found to be polymorphic. this genetic variation allows for a more intricate repertoire that enables the host to withstand microbial challenges. while this may be advant ... | 2008 | 18176606 |
| helicobacter pylori activates gastric epithelial cells to produce interleukin-8 via protease-activated receptor 2. | recently, it has been shown that serine proteases derived from microorganisms stimulate epithelial cells to produce inflammatory mediators through protease-activated receptor (par). we investigated the involvement of par2 in the interleukin (il)-8 production by helicobacter pylori-infected gastric epithelial cells. | 2008 | 18196899 |
| helicobacter pylori protein response to human bile stress. | the ability of helicobacter pylori to tolerate bile is likely to be important for its colonization and survival in the gastrointestinal tract of humans. as bile can be acidified after reflux into the low ph of the human stomach, the inhibitory effect of fresh human bile with normal appearance on h. pylori before and after acidification was tested first. the results showed that acidification of bile attenuated its inhibitory activity towards h. pylori. next, the protein profiles of h. pylori unde ... | 2008 | 18201979 |
| ccl28 is increased in human helicobacter pylori-induced gastritis and mediates recruitment of gastric immunoglobulin a-secreting cells. | human helicobacter pylori infection gives rise to an active chronic gastritis and is a major risk factor for the development of duodenal ulcer disease and gastric adenocarcinoma. the infection is accompanied by a large accumulation of immunoglobulin a (iga)-secreting cells in the gastric mucosa, and following mucosal immunization only h. pylori-infected volunteers mounted a b-cell response in the gastric mucosa. to identify the signals for recruitment of gastric iga-secreting cells, we investiga ... | 2008 | 18426876 |
| discovering differential protein expression caused by caga-induced erk pathway activation in ags cells using the seldi-proteinchip platform. | to identify the protein expression differences related to the caga-induced erk pathway activation in ags cells. | 2008 | 18203287 |
| long-term follow-up of gastrectomized patients with mucosa-associated lymphoid tissue lymphoma: need for a revisit of surgical treatment. | gastric mucosa-associated lymphoid tissue (malt) lymphoma is characterized by multifocality of the tumors, which often scatter over the mucosa of the stomach and adjacent upper gastrointestinal tract, and is therefore theoretically not curable by surgical resection. | 2008 | 18216531 |
| chronic active gastritis in x-linked lymphoproliferative disease. | gastric lesions in primary constitutive immune deficiencies include multifocal atrophic gastritis, erosive pangastritis, and a pattern of gastric lesions reminiscent of graft-versus-host disease. we describe the genetic anomalies in 2 monozygotic twins with an x-linked lymphoproliferative disease (xlp; mim 308240), a rare familial setting of high susceptibility to epstein-barr virus (ebv). since early childhood, both twin brothers exhibited a severe chronic active atrophic pangastritis. a germli ... | 2008 | 18223336 |
| effects of helicobacter pylori infection on gastric mucin expression. | this study was performed to determine the gastric distributions of muc5ac and muc6 depending on helicobacter pylori (h. pylori) infection, and to evaluate whether the expressions of muc5 and muc6 change in h. pylori-associated gastroduodenal diseases. in addition, muc5ac and muc6 expressional changes were investigated before and after h. pylori eradication. | 2008 | 18097286 |
| role of toll-like receptors in gastrointestinal malignancies. | mounting evidence supports the tenet that innate immune responses to luminal microbes participate in the development of gastrointestinal malignancies. the gastrointestinal tract is relatively unique in that it has evolved in the presence of diverse enteric microflora. intestinal flora is required to develop a normal adaptive immune response in the periphery. with the characterization of the innate immune system, we have begun to understand the adaptations the intestine has made to the microbiota ... | 2008 | 18176605 |
| probiotics, prebiotics, and synbiotics. | according to the german definition, probiotics are defined viable microorganisms, sufficient amounts of which reach the intestine in an active state and thus exert positive health effects. numerous probiotic microorganisms (e.g. lactobacillus rhamnosus gg, l. reuteri, bifidobacteria and certain strains of l. casei or the l. acidophilus-group) are used in probiotic food, particularly fermented milk products, or have been investigated--as well as escherichia coli strain nissle 1917, certain entero ... | 2008 | 18461293 |
| the effects of cyclooxygenase2-prostaglandine2 pathway on helicobacter pylori-induced urokinase-type plasminogen activator system in the gastric cancer cells. | urokinase-type plasminogen activator (upa) and its receptor (upar) play an important role in the destruction of the extracellular matrix and basement membrane. the induction of upa and upar in the gastric cancer cells with h. pylori has been demonstrated previously. the involvement of cox-2-pge2 pathway in the upa system (upa and upar) expression is unclear. | 2008 | 18466392 |