Publications

antibiotic treatment in lyme borreliosis.	before the etiology of lyme borreliosis became known, its human manifestations were most often treated with penicillin. treatment was mostly successful, in spite of the fact that lower doses were generally used, but the doses were given with shorter intervals. lately, other antibiotics have been tried, more or less successfully, but there is still no consensus as to which--if any--antibiotic is superior to others, or if one manifestation of the infection is better treated than another with a par ...	1991	1947804
susceptibility of iron-loaded borrelia burgdorferi to killing by hydrogen peroxide and human polymorphonuclear leucocytes.	borrelia burgdorferi grew more slowly in iron-depleted than in iron-sufficient media. the addition of increasing concentrations of iron stimulated borrelial growth and resulted in the intracellular accumulation of this element. compared with iron-starved borrelia, iron-enriched organisms showed enhanced sensitivity to hydrogen peroxide. intracellular iron-content did not, however, influence susceptibility to killing by human polymorphonuclear leucocytes [corrected].	1991	1874405
ixodes scapularis (acari: ixodidae) in kansas: a new distribution record.	the blacklegged tick, ixodes scapularis say, previously known to occur only in the extreme southeastern corner of kansas, has been collected in douglas and jefferson counties in the northeast. the new collections extend the northern range of this species in kansas by approximately 240 km. the role of i. scapularis as a vector of the lyme borreliosis spirochete, borrelia burgdorferi, makes this a noteworthy extension of its known range. the proximity of these new collection sites to large centers ...	1991	1770524
babesia microti, human babesiosis, and borrelia burgdorferi in connecticut.	babesia microti was isolated from a white-footed mouse (peromyscus leucopus) that was captured in southeastern connecticut in 1988, when the first human case of babesiosis acquired in connecticut was recognized. to date, 13 cases of babesiosis have been reported in connecticut, the largest number of human cases reported on the mainland united states. two of nine patients quiried remembered a prior tick bite. since babesia parasites are known to be vectored only by ticks, we surmise that 12 of th ...	1991	1757548
[transient complete av block as a sequela of borrelia myocarditis].	a 49-year-old man suddenly developed dyspnoea, sweating, fever (up to 38.5 degrees c), vertigo and angina. after emergency admittance to hospital the ecg showed 3 degrees a-v block, requiring temporary pacemaker insertion. the patient reported that a month before he had been bitten, probably by a tick. serological tests demonstrated a recent borrelia infection (rise of igg antibody titre to 1:2048, igm antibody titre to 1:128). coronary angiography excluded any haemodynamically significant coron ...	1992	1735375
immunological characterization of a low molecular mass polypeptidic antigen of borrelia burgdorferi.	the presence of a low molecular mass polypeptidic antigen in borrelia burgdorferi was described. the protein was exposed at the bacterial surface since it was clearly identified by mab 3h4 using the immunofluorescence test performed with living bacteria. this antigen was cleaved by proteinase k treatment, whereas it was resistant to the action of chymotrypsin, trypsin and thermolysin. western blotting analysis of the immunological reactivity of this antigenic structure performed using monoclonal ...	1991	1725956
t cell and antibody reactivity with the borrelia burgdorferi 60-kda heat shock protein in lyme arthritis.	the reactivity of cloned t cells and serum antibodies, obtained from patients with chronic lyme arthritis, with expressed recombinant b. burgdorferi 60-kda heat shock protein homologue (hsp60) was analyzed. the expressed recombinant borrelia burgdorferi hsp60 was bound by antibodies in the sera of patients with lyme arthritis, but not by control sera. a t cell clone (cr253), isolated from one of four patients examined, exhibited an hla-dr2 restricted proliferative response to the expressed recom ...	1991	1709664
antibodies to borrelia burgdorferi in rodents in the eastern and southern united states.	serologic studies were conducted to determine whether white-footed mice (peromyscus leucopus) and cotton mice (peromyscus gossypinus) contained serum antibodies to borrelia burgdorferi, the causative agent of lyme borreliosis. enzyme-linked immunosorbent assays detected antibodies to this spirochete in 35.7 and 27.3% of 56 p. leucopus and 535 p. gossypinus serum samples, respectively, collected in connecticut, north carolina, south carolina, georgia, florida, alabama, and mississippi. antibody t ...	1992	1624561
distribution, density, and lyme disease spirochete infection in ixodes dammini (acari: ixodidae) on white-tailed deer in maryland.	a statewide survey of ticks parasitizing white-tailed deer was carried out in maryland during november 1989 to assess the status of the deer tick, ixodes dammini spielman, clifford, piesman & corwin, the major vector of lyme disease in the northeastern united states. ticks were collected from deer carcasses brought in by hunters at 23 check stations (one per county). a total of 3,437 i. dammini were collected from 538 of 1,281 deer (42%), together with 2,013 dermacentor albipictus (packard) and ...	1992	1552529
population genetic analysis of borrelia burgdorferi isolates by multilocus enzyme electrophoresis.	fifty borellia burgdorferi strains isolated from humans and ticks in europe and the united states were analyzed by multilocus enzyme electrophoresis. eleven genetic loci were characterized on the basis of the electrophoretic mobilities of their products. ten loci were polymorphic. the average number of alleles per locus was 5.9, with a mean genetic diversity of 0.673 among electrophoretic types (ets). the strains were grouped into 35 ets constituting three main divisions (i, ii, and iii) separat ...	1992	1548090
in vitro and in vivo induction of tumor necrosis factor alpha by borrelia burgdorferi.	tumor necrosis factor alpha (tnf-alpha) is an immunoregulatory cytokine with many biological activities including the mediation of inflammation. we examined sera and synovial fluids from patients seropositive for infection with borrelia burgdorferi using a radioimmunoassay specific for tnf-alpha. significant elevation of tnf-alpha was found in the sera and synovial fluids of patients examined, while controls showed no elevation. sera of mice infected with b. burgdorferi contained elevated levels ...	1992	1541526
serodiagnosis of lyme borreliosis by western immunoblot: reactivity of various significant antibodies against borrelia burgdorferi.	the significance of various antibodies against borrelia burgdorferi was studied by western blot (immunoblot) by using 578 human serum samples. the proteins regularly detected by using samples from patients with lyme borreliosis were those with bands with molecular masses of 94, 83, 75, 66, 60, 55, 46, 41, 39, 34, 31, 29, 22, and 17 kda. the detectable frequencies of most of these proteins appeared to be significantly different between the sera from patients with lyme borreliosis and those from n ...	1992	1537905
borrelia burgdorferi stimulates release of interleukin-1 activity from bovine peripheral blood monocytes.	infection with borrelia burgdorferi is suspected to be a cause of lameness and arthritis in cattle. interleukin-1 (il-1) activity has been detected in joint fluids from human patients affected by various arthritides, including lyme arthritis. in addition, human monocytes and murine macrophages have been reported to release il-1 activity when incubated with b. burgdorferi in vitro. to address a possible mechanism by which b. burgdorferi might cause a bovine arthritic syndrome, we determined wheth ...	1992	1500171
cluster of tick-borne infections at fort chaffee, arkansas: rickettsiae and borrelia burgdorferi in ixodid ticks.	human intrusion into pristine habitats increases the likelihood of acquiring infectious agents from potentially infective ticks. as part of a larger human serological investigation into tick-borne illnesses, 3,000 ixodid ticks were collected during may, august, and november 1990 at fort chaffee, arkansas. ticks were examined to determine whether they harbor rickettsiae, ehrlichiae, and borrelia burgdorferi, and to assess relationship to human exposure to tick-borne infections at fort chaffee, ar ...	1992	1495077
the 93-kilodalton protein of borrelia burgdorferi: an immunodominant protoplasmic cylinder antigen.	using immunoblots, we identified proteins of borrelia burgdorferi recognized by sera from 62 patients with either acute or chronic lyme disease. in all groups studied, the 41-kda flagellar protein and a relatively minor 93-kda protein (p93) were the most commonly recognized antigens in patients with acute and chronic disease due to b. burgdorferi. a murine monoclonal antibody (mab 181.1) was developed against p93, and the antigen was detected by immunoblot analysis in four european and american ...	1992	1398941
molecular detection of persistent borrelia burgdorferi in a man with dermatomyositis.	a 40-year-old white man with a several year history of various immunologic disorders, including anti-jo-1 autoantibody positive dermatomyositis, developed clinical lyme disease after being biten by a tick. the patient was treated with oral tetracycline and his initial symptoms resolved; however, he suffered an exacerbation of his muscle disease which was difficult to control despite cytotoxic therapy. antibiotic therapy was reinstituted after borrelia burgdorferi was detected in the patient's pe ...	1992	1395222
lyme disease.	lyme disease is a multisystem disorder caused by the spirochete borrelia burgdorferi. it is transmitted to human and animal hosts primarily by ticks of the ixodes ricinis complex. recognition of its characteristic skin and eye manifestations facilitates diagnosis and treatment.	1992	1395157
sequence homology between spirochaete flagellin and human myelin basic protein.		1992	1382434
borrelia burgdorferi hsp70 homolog: characterization of an immunoreactive stress protein.	the gene encoding an immunoreactive borrelia burgdorferi hsp70 homolog was isolated and characterized. the predicted amino acid sequence of this spirochetal protein confirms that this gene encodes a member of the hsp70 family of proteins. although there appears to be a single copy of this gene on the spirochetal chromosome, two distinct transcripts hybridizing to the hsp70 probe are detected in rna isolated from b. burgdorferi. the amount of spirochetal hsp70 rna transcripts is shown to be therm ...	1992	1379988
epitopes on the outer surface protein a of borrelia burgdorferi recognized by antibodies and t cells of patients with lyme disease.	we have characterized immunogenic epitopes of the 31-kda outer surface protein a (ospa) protein of borrelia burgdorferi, which is a major surface ag of the spirochete causing lyme disease. full length and truncated forms of rospa proteins were expressed in escherichia coli, and their reactivities with antibodies and human t cell clones isolated from patients with lyme disease were determined. the epitopes recognized by three of four ospa-reactive t cell clones are contained within the 60 cooh-te ...	1992	1370170
comparison of whole-cell antibodies and an antigenic flagellar epitope of borrelia burgdorferi in serologic tests for diagnosis of lyme borreliosis.	a recombinant protein (p41-g) of an antigenic region of flagellin was used in a standard and amplified enzyme-linked immunosorbent assay (elisa) to detect antibodies to borrelia burgdorferi, the causative agent of lyme borreliosis. comparable sensitivities (88 to 94%) were noted when sera from 17 persons who had erythema migrans and antibodies to whole-cell b. burgdorferi were tested against the p41-g antigen. in tests of a second study group of 36 persons who had erythema migrans but no detecta ...	1992	1280650