Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| detection of specific reticuloendotheliosis virus sequence and protein from rev-integrated fowlpox virus strains. | the detection is described of reticuloendotheliosis virus (rev) protein in tissue culture of chicken embryonated cells (cefs) infected with field isolates of fowl poxvirus (fpv). by the polymerase chain reaction (pcr), five out of the six field isolates, but two out of the seven vaccine strains of fpv, were found to have had a 291 bp repeat sequence of rev-ltr integrated in their genomic dna. an immunofluorescence (if) method was employed using a monoclonal antibody (mab) known to specify strain ... | 2003 | 12757926 |
| fowlpox virus infection causes a lymphoproliferative response in chickens. | while attenuated fowlpox virus (fpv) strains are widely used for vaccination of chickens and turkeys for prevention of fowlpox, recombinant fpv expressing various foreign genes have been evaluated for their ability to offer protection against various diseases in poultry as well as mammals. little is known regarding the cell-mediated immune responses to fpv infection. in this study, immune response in chickens infected with a virulent and a vaccine strain of fpv were compared by a lymphoprolifera ... | 2003 | 12828873 |
| characterization of an avianpox virus isolated from an andean condor (vultur gryphus). | a novel pox virus, condorpox virus (cpv) isolated from the spleen of an andean condor (vultur gryphus) by inoculation of chorioallantoic membranes (cam) of specific pathogen free (spf) chicken embryos was compared biologically, antigenically and genetically with fowlpox virus (fpv), the type species of the genus avipoxvirus. susceptible chickens inoculated with cpv developed only mild localized lesions but were not protected against subsequent challenge with fpv. based on western blotting, in ad ... | 2003 | 14559171 |
| vaccination against fowlpox virus via drinking water. | the oral vaccination against fowlpox was investigated via drinking water containing the f132-c strain of fowlpox virus to be effective even though the vaccine virus-titer was 10(4) tcid (50)/dose each time. when the virus-titer of the f132-c strain was 10(4-5 )tcid(50)/dose per single drinking water vaccination, 90% or more of chickens were not protected, however, they were protected when vaccinated twice via drinking water. a weak immune response occurred by a slight infection after the first v ... | 2003 | 14600354 |
| severe acute respiratory syndrome vaccine development: experiences of vaccination against avian infectious bronchitis coronavirus. | vaccines against infectious bronchitis of chickens (gallus gallus domesticus) have arguably been the most successful, and certainly the most widely used, of vaccines for diseases caused by coronaviruses, the others being against bovine, canine, feline and porcine coronaviruses. infectious bronchitis virus (ibv), together with the genetically related coronaviruses of turkey (meleagris gallopovo) and ring-necked pheasant (phasianus colchicus), is a group 3 coronavirus, severe acute respiratory syn ... | 2003 | 14676007 |
| protection from infectious bursal disease virus (ibdv)-induced immunosuppression by immunization with a fowlpox recombinant containing ibdv-vp2. | immunosuppression resulting from infectious bursal disease virus (ibdv) infection has critical health and welfare implications for birds, yet it is incompletely understood and largely overlooked as a measure of vaccine efficacy. the ability of a fowlpoxvirus recombinant (fpibd1) containing the vp2 protein of ibdv to protect against ibdv-induced immunosuppression was investigated by measuring the convalescent chicken's ability to mount antibody responses to ibdv infection, and to inactivated ibdv ... | 2003 | 14676010 |
| enhanced cd8+ t cell immune responses and protection elicited against plasmodium berghei malaria by prime boost immunization regimens using a novel attenuated fowlpox virus. | sterile immunity can be provided against the pre-erythrocytic stages of malaria by ifn-gamma-secreting cd8(+) t cells that recognize parasite-infected hepatocytes. in this study, we have investigated the use of attenuated fowlpox virus (fpv) strains as recombinant vaccine vectors for eliciting cd8(+) t cells against plasmodium berghei. the gene encoding the p. berghei circumsporozoite (pbcs) protein was inserted into an fpv vaccine strain licensed for use in chickens, webster's fpv, and the nove ... | 2004 | 14978115 |
| the efficacy of recombinant fowlpox vaccine protection against marek's disease: its dependence on chicken line and b haplotype. | earlier studies have shown that the b haplotype has a significant influence on the protective efficacy of vaccines against marek's disease (md) and that the level of protection varies dependent on the serotype of md virus (mdv) used in the vaccine. to determine if the protective glycoprotein gene gb is a basis for this association, we compared recombinant fowlpox virus (rfpv) containing a single gb gene from three serotypes of mdv. the rfpv were used to vaccinate 15.b congenic lines. nonvaccinat ... | 2004 | 15077806 |
| the dna repair enzyme, cpd-photolyase restores the infectivity of uv-damaged fowlpox virus isolated from infected scabs of chickens. | fowlpox virus (fwpv), an important pathogen of poultry, replicates very efficiently in the featherless areas of skin, and persists in dried and desiccated scabs for prolonged periods. although the molecular mechanisms underlying the stability of the virus are not completely known, we recently identified the presence of a virus-encoded novel dna repair enzyme, cpd-photolyase, in fwpv. this enzyme repairs the ultraviolet (uv)-induced pyrimidine dimers, converting them to monomers using photons fro ... | 2005 | 15936904 |
| [construction of recombinant fowlpox virus coexpressing gp120 of chinese hiv-1 strain and il-18 and its immunogenicity in mice]. | to screening out chinese vaccine candidate against hiv-1, chinese vaccine strain 282e4 of fowlpox virus was used as the vector to construct the recombinant fowlpox virus (rfpv) coexpressing gp120 of chinese hiv-1 strain and il-18, and the recombinant virus was indentified by pcr and western blot. the specific dna fragment could be amplified by pcr from the genome of rfpv. western blot analysis showed that gp120 and il-18 could be expressed not only in chicken embryo fibroblast (cef) cells infect ... | 2004 | 15971601 |
| [influence of nonessential region on protective efficacy of recombinant fowlpox viruses]. | because of the interference of maternal antibodies, the recombinant fowlpox virus (rfpv) vaccine has not been used widely. the selection of a well-defined fpv nonessential region might be a desirable way to solve this problem. two pairs of primers were designed according to the genome of a pathogenic fpv to amplify two flanking regions (fpv1 and fpv2) of the supposed nonessential region by pcr, and then a series of plasmid vectors were constructed to generate the expression vector pp12ls, which ... | 2005 | 15989226 |
| construction and characterization of recombinant fowlpox virus coexpressing hiv-1(cn) gp120 and il-2. | the acquired immunodeficiency syndrome (aids) has now become one of the most serious infectious disease in the world, the safe and effective aids vaccines would be the best tools to control the disease. fowlpox virus has been studied recently as a potential vector for the delivery of heterologous vaccine antigen. in this study, a recombinant fowlpox virus coexpressing gp120 of chinese prevalent hiv-1 strain and il-2 was constructed and the cellular immune responses against hiv-1 gp120 in balb/c ... | 2005 | 16029899 |
| characterization of canarypox-like viruses infecting endemic birds in the galápagos islands. | the presence of avian pox in endemic birds in the galápagos islands has led to concern that the health of these birds may be threatened by avipoxvirus introduction by domestic birds. we describe here a simple polymerase chain reaction-based method for identification and discrimination of avipoxvirus strains similar to the fowlpox or canarypox viruses. this method, in conjunction with dna sequencing of two polymerase chain reaction-amplified loci totaling about 800 bp, was used to identify two av ... | 2005 | 16107669 |
| [construction of recombinant fowlpox virus expressing chicken il-2 and assay of biologic activity of the product in vitro]. | in order to determine the adjuvant effects of the chicken il-2 (chil-2) on new generation vaccines, chil-2 gene was amplified from cona-stimulated chicken spleen cells by rt-pcr and was directionally inserted into fowlpox virus (fpv) transferring vector p1175 under the control of fpv early/late promoter (pe/l), resulting in recombinant transferring vector p1175il2. then the p1175il2 plasmid was transfected into chicken embryo fibroblasts (cef) pre-infected with wild type fpv to generate recombin ... | 2004 | 16108505 |
| the phytochemical composition and in vitro antiviral activity of decoctions from galls of guiera senegalensis j.f. gmel. (combretaceae) and their relative non-toxicity for chickens. | aqueous decoctions obtained from the galls of guiera senegalensis were screened to determine their phytochemical composition and in vitro antiviral activity against fowlpox virus. in addition, we wanted to investigate the toxic effects, if any, of crude extracts in chickens. steroids as well as cardiac glycosides not previously reported, an alkaloid, polyphenols and saponins were detected in the various fractions of organic solvents used for extracting the decoctions. antiviral activity was dete ... | 2005 | 16137128 |
| in ovo inhibition of fowlpoxvirus replication by a gall extract from guiera senegalensis. | several field isolates of fowlpoxvirus (fpv) from burkina faso, west africa, were isolated and partly evaluated by molecular analysis. in addition, the in ovo antiviral activity against fpv of a gall extract from guiera senegalensis was determined. three viral isolates were obtained from suspected fowlpox cases after passage in embryonating chicken eggs and their poxviral identity confirmed by electron microscopy. all isolates were found to be pathogenic for chicks and all grew well in cell cult ... | 2005 | 16191693 |
| construction and characterization of a fowlpox virus field isolate whose genome lacks reticuloendotheliosis provirus nucleotide sequences. | fowlpox virus (fwpv) has been isolated from vaccinated chicken flocks during subsequent fowlpox outbreaks that were characterized by a high degree of mortality and significant economic losses. this inability of current vaccines to induce adequate immunity in poultry could be reflective of an antigenic and/or biologic distinctiveness of fwpv field isolates. in this regard, whereas an infectious reticuloendotheliosis virus (rev) provirus is present in the majority of the field viruses' genomes, on ... | 2005 | 16252496 |
| [selection of recombinant fowlpox virus coexpressing hiv-1 gag-gp120 and il-6]. | to construct the recombinant fowlpox virus (rfpv) coexpressing hiv-1 gag-gp120 and hil-6. | 2005 | 16261213 |
| immunogenicity of fowlpox virus expressing the avian influenza virus h5 gene (trovac aiv-h5) in cats. | vaccination of cats with fowlpox virus expressing the avian influenza (ai) virus h5 hemagglutinin gene (trovac ai) resulted in detectable hemagglutination inhibition (hi) antibody responses to the homologous a/turkey/ireland/1378/83 (h5n8) (a/tky/ire/83) ai virus antigen. the hi antibody responses to heterologous a/chicken/indonesia/7/03 (h5n1) (a/ck/indonesia/03) ai virus antigen were also detected in all vaccinated cats, but only after booster vaccinations. the vaccine described in this study ... | 2005 | 16275953 |
| [construction, genetic stability and protective efficacy of recombinant fowlpox virus expressing hemagglutinin gene of h5n1 subtype avian influenza virus]. | construction, genetic stability and protective efficacy of a recombinant fowlpox virus expressing hemagglutinin (ha) gene (rfpv-ha) of h5 subtype avian influenza virus were conducted. the results indicated that the recombinant fowlpox virus was obtained and purified by blue plaque selection. ha expressed by rfpv-ha was identified by indirect immunofluorescence array. the rfpv-ha could express reporter gene lacz and ha gene after fifteen successive passages in chicken embryo fibroblast. the rfpv- ... | 2003 | 16276892 |
| development of a recombinant fowlpox virus vector-based vaccine of h5n1 subtype avian influenza. | the genetic stability of the recombinant fowlpox virus (named rfpv-ha-na) was confirmed by serial passage on chicken embryo fibroblast (cef) cells. the immune efficacy, safety, the minimum immunising dose, the time of immunity induced and the immune duration of the vector-based vaccine was evaluated in specific-pathogen-free (spf) chickens. the recombinant virus vaccine containing 100 plaque form units (pfu) could induce complete protection against challenge with h5n1 highly pathogenic avian inf ... | 2006 | 16447503 |
| [recombinant fowlpox virus coexpressing ha and na gene from subtype h5n1 of avian influenza virus and its protective efficacy]. | the hemagglutinin (ha) and neuraminidase(na)gene from subtype h5n1 avian influenza virus were directly inserted into the transferring vector plls, resulting in the recombinant transferring vector p11sh5ana. then p11sh5ana was transfected into the chicken embryo fibroblasts (cef), which was pre-infected with wild type fowlpox virus, to generate the recombinant fowlpox virus coexpressing h5a and na (rfpv-11sh5ana). by selection of blue plaques on the cef, rfpv-11sh5ana was obtained and purified. e ... | 2006 | 16579476 |
| in ovo dna immunisation followed by a recombinant fowlpox boost is fully protective to challenge with virulent ibdv. | the aim of this study was to investigate the potential use of dna vaccination delivered in ovo for protecting against challenge with infectious bursal disease virus (ibdv). using a plasmid expressing the beta-galactosidase gene, dna was successfully delivered to the embryo after in ovo injection and localises to the proventriculus and thymus. the coding sequence for the immunogenic ibdv protein, vp2, was cloned into pci-neo, creating pci-vp2. complete protection against ibdv was obtained by prim ... | 2006 | 16621184 |
| construction and immunogenicity of recombinant fowlpox vaccines coexpressing ha of aiv h5n1 and chicken il18. | cdnas of the ha genes of subtype h5n1 aiv were fused to form a single open reading frame, designated h5ha-h7ha. the h5ha-h7ha cdna and chicken interleukin-18 (il18) were inserted into the fowlpox virus (fpv) expression vector puta-16-lacz to produce putal-h5-h7-il18. cdna of h5n1 aiv ha was inserted into the fpv expression vector puta2 to create the recombinant expression plasmid puta2-h5. plasmids were then co-transected into cef cells. the two recombinant fowlpox viruses (rfpv) were produced b ... | 2006 | 16621199 |
| evaluation of immune effects of fowlpox vaccine strains and field isolates. | the immune effects of fowlpox virus (fpv) field isolates and vaccine strains were evaluated in chickens infected at the age of 1 day and 6 weeks. the field isolates and the obsolete vaccine strain (fpv s) contained integrated reticuloendotheliosis virus (rev) provirus, while the current vaccine strain (fpvst) carries only rev ltr sequences. an indirect antibody elisa was used to measure the fpv-specific antibody response. the non-specific humoral response was evaluated by injection of two t-cell ... | 2006 | 16650660 |
| antitumor effects of a recombinant fowlpox virus expressing apoptin in vivo and in vitro. | apoptin is a chicken anemia virus-derived, p53-independent, bcl-2-insensitive apoptotic protein with the ability to specifically induce apoptosis in tumor cells. to explore the use of the apoptin gene in cancer gene therapy, we constructed a recombinant fowlpox virus expressing the apoptin protein (vfv-apoptin) and compared the tumor-killing activity of the recombinant virus with that of wild-type fowlpox virus in the human hepatoma cell line hepg2. we found that although cells were somewhat res ... | 2006 | 17036330 |
| detection of reticuloendotheliosis virus in live virus vaccines of poultry. | in vitro and in vivo assays have been used for the detection of reticuloendotheliosis virus (rev) in live virus vaccines of poultry. the presence of rev is confirmed by the demonstration of viral antigen or provirus in chicken embryo fibroblasts (cefs) or in specific-pathogen-free chickens inoculated with vaccine. using rev polyclonal or monoclonal antibodies, cefs inoculated with vaccines can be examined for rev by immunofluorescence or immunoperoxidase staining methods. cell lysates from such ... | 2006 | 17058506 |
| full genome sequence and some biological properties of reticuloendotheliosis virus strain apc-566 isolated from endangered attwater's prairie chickens. | reticuloendotheliosis virus (rev) causes runting, high mortality, immunosuppression, and chronic neoplasia associated with t and/or b cell lymphomas in a variety of domestic and wild birds, including attwater's prairie chickens (apc) (tympanuchus cupido attwateri). the complete proviral sequence of a recent rev isolate from apc (rev apc-566) was determined. this virus was isolated from an apc maintained in captivity in a reproduction program intended to avoid its extinction. rev apc-566 was dete ... | 2007 | 17098316 |
| development and use of fowlpox vectored vaccines for avian influenza. | the avian influenza (ai) vaccine designated trovac-aiv h5 (trovac-h5) contains a live recombinant fowlpox rec. (fp) recombinant (recfp), expressing the hemagglutinin (ha) gene of an ai h5 subtype isolate. this recombinant vaccine was granted a license in the united states for emergency use in 1998 and full registration in mexico, guatemala, and el salvador where over 2 billion doses have been administered. one injection of trovac-h5 protects chickens against ai-induced mortality and morbidity fo ... | 2006 | 17135511 |
| [construction and characterization of a recombinant fowlpox virus co-expressing f, hn genes of newcastle disease virus and gb gene of infectious laryngnotracheitis virus]. | the fusion (f) and haemagglutinin-neuraminidase (hn) genes of newcastle disease virus (ndv) and the glycoprotein b (gb) gene of infectious laryngothracheitis virus (iltv) as well as a lacz reporter gene were all inserted into a nonessential gene of fowlpox virus (fpv) 017 strain by homologous recombination. the ndv and iltv genes were each under the control of a fowlpox virus immediate early/late promoter (lp2ep2) while the lacz reporter gene expression cassette was regulated by a p11 late promo ... | 2006 | 17168315 |
| [construction and screening of recombinant fowlpox virus expressing eimeria tenella f2 hybrid strain so7 gene]. | to construct the recombinant fowlpox virus expressing eimeria tenella f2 hybrid strain so7 gene. | 2006 | 17361816 |
| [self-excising reporter gene in recombinant fowlpox virus expressing h5 hemagglutinin gene of influenza a virus using cre-loxp systerm]. | hemagglutinin gene of subtype h5 avian influenza virus was amplified by polymerase chain reaction to construct expression cassette containing fpv early, late promoter and sv40 polya tail. then delivery vector was constructed by subcloning hemagglutinin gene of subtype h5 and gfp gene into fowlpox virus recombinant arm. the delivery vector and lipid were transfected into cef cells preinfected with fpv 282e4 strain virus. recombinant fowlpox virus expressing the green fluorescence protein and hema ... | 2007 | 17672322 |
| fowlpox virus encodes a bcl-2 homologue that protects cells from apoptotic death through interaction with the proapoptotic protein bak. | poxviruses are renowned for encoding numerous immunomodulatory proteins capable of undermining potent immune defenses. one effective barrier against infection is apoptosis, a process controlled at the mitochondria by pro- and antiapoptotic members of the highly conserved bcl-2 family of proteins. although poxviruses are known to encode an array of effective inhibitors of apoptosis, members of the avipoxvirus genus, which includes fowlpox virus, encode proteins with bcl-2 homology. here, we show ... | 2007 | 17686864 |
| detection and differentiation of re-emerging fowlpox virus (fwpv) strains carrying integrated reticuloendotheliosis virus (fwpv-rev) by real-time pcr. | current strains of fowlpox virus (fwpv) carrying circulating reticuloendotheliosis virus (fwpv-rev) sequence are becoming more pathogenic to poultry. this is evidenced by the fact that vaccination with current available fwpv vaccines provides limited protection against them. to characterize rev insertions in a collection of both older and more recent field isolates, we developed three different types of adjacent oligoprobes and primer sets from specific genomic locations of fwpv and rev: rev-env ... | 2008 | 17913400 |
| creation of diversity in the animal virus world by inter-species and intra-species recombinations: lessons learned from poultry viruses. | the biological diversity within viruses is one of the largest found in all other forms of nature. many mechanisms contribute to virus diversity and include incorporating genetic material from the host, recombination between viruses belonging to the same or to a different family, and even recombination between viruses normally infecting different hosts. in particular, avian viruses can utilize all three of these mechanisms to generate new viruses. it is well documented that recombinations can occ ... | 2008 | 17922180 |
| improvements to the hemagglutination inhibition test for serological assessment of recombinant fowlpox-h5-avian-influenza vaccination in chickens and its use along with an agar gel immunodiffusion test for differentiating infected from noninfected vaccinated animals. | in general, avian influenza (ai) vaccines protect chickens from morbidity and mortality and reduce, but do not completely prevent, replication of wild ai viruses in the respiratory and intestinal tracts of vaccinated chickens. therefore, surveillance programs based on serological testing must be developed to differentiate vaccinated flocks infected with wild strains of ai virus from noninfected vaccinated flocks in order to evaluate the success of vaccination in a control program and allow conti ... | 2007 | 17992929 |
| concurrent infection in chickens with fowlpox virus and infectious laryngotracheitis virus as detected by immunohistochemistry and a multiplex polymerase chain reaction technique. | a concurrent infection of chickens with infectious laryngotracheitis virus (iltv), a herpesvirus, and fowlpox virus (fwpv), an avipoxvirus, is described. two techniques, an immunohistochemistry (ihc) technique and a multiplex polymerase chain reaction (pcr), were used to examine 11 tissue samples from chickens clinically diagnosed as fwpv-infected, but only ihc was used to examine six tissue-paraffin blocks prepared from turkeys suspected of having fwpv infection. by multiplex pcr, both fwpv and ... | 2007 | 17992932 |
| avipoxvirus in blackcaps (sylvia atricapilla). | from july to september 2005, 1075 wild birds of 37 species were mist-netted at a location in the north-eastern part of the czech republic. the birds were examined for the presence of avipoxvirus lesions. this was demonstrated by electron microscopy in skin lesions in nine of 244 blackcaps (sylvia atricapilla) examined (4% prevalence). blackcaps skin bioptates were processed using the ultrathin section method. in skin bioptates, avipoxviruses were demonstrated in intracytoplasmic inclusions where ... | 2008 | 18202957 |
| eimeria tenella: construction of a recombinant fowlpox virus expressing rhomboid gene and its protective efficacy against homologous infection. | a recombinant fowlpox virus (rfpv) expressing the eimeria tenella rhomboid gene was constructed and its protective efficacy against homologous infection in chickens determined. three-day-old-specific pathogen free (spf) chickens were immunized s.c. with 10(2) plaque forming units (pfu), 10(4) pfu, or 10(6) pfu of rfpv-rhomboid, and challenged with 5x10(4) homologous sporulated oocysts 14 days post-immunization (p.i.). the specific antibody response and lymphocyte proliferation were measured 1, 2 ... | 2008 | 18261729 |
| [construction of recombinant fowlpox virus expressing e0 gene of classical swine fever virus shimen strain and the animal immunity experiment]. | the csfv e0 gene was amplified from the plasmid pmd18-t-e0 by pcr and cloned into the fpv-p11 and fpv-psy. the identified recombinant dna was transfected into chicken embryo fibroblasts (cef) to package fowlpox virus. e0 gene was confirmed to be integrated into the genome of recombinant fowlpox virus by pcr, and western blot was employed for detection of e0 expression in the chicken embryo fibroblasts infected with recombinant fowlpox virus . the results of elisa showed that systemic immune resp ... | 2008 | 18320824 |
| protection of chickens from newcastle disease and infectious laryngotracheitis with a recombinant fowlpox virus co-expressing the f, hn genes of newcastle disease virus and gb gene of infectious laryngotracheitis virus. | a recombinant fowlpox virus (rfpv) coexpressing the newcastle disease virus (ndv) fusion and hemagglutinin-neuraminidase genes and infectious laryngothracheitis virus (iltv) glycoprotein b gene was constructed. this virus was then evaluated for its ability to protect specific-pathogen-free (spf) chickens against clinical symptoms and death after challenge by virulent ndv and iltv. spf chickens were grouped and vaccinated with the rfpv and commercial ndv (la sota) and iltv attenuated live vaccine ... | 2008 | 18459306 |
| field isolates of fowlpox virus contaminated with reticuloendotheliosis virus. | the polymerase chain reaction (pcr) method was used to examine samples from field cases of fowlpox for the presence of reticuloendotheliosis virus (rev). the s-strain fowlpox vaccine, known to be contaminated with rev, served as a positive control. fowlpox virus was grown from field samples and vaccines by inoculation of embryonated hen eggs by the chorioallantoic membrane (cam) route. dna was extracted from the cam lesions and examined for rev proviral sequences using primers specific for the l ... | 1998 | 18483965 |
| recombinant fowlpox virus elicits transient cytotoxic t cell responses due to suboptimal innate recognition and recruitment of t cell help. | recombinant fowlpox viruses (fpvs) have been used in a variety of vaccine strategies; however strong data clearly demonstrating the characteristics of the strength and nature of the resultant immune response elicited by these vectors are lacking. by utilising a recombinant variant of fpv which expresses the nominal antigen chicken ovalbumin (ova), and assessing innate fpv- and ova-specific adaptive immune responses, we show that recombinant fpv induces a rapid type i interferon (ifn) response, m ... | 2008 | 18541346 |
| fowlpox vaccination: routes of inoculation and pathological effects. | chickens were vaccinated against fowlpox via the wing web, oral route, drinking water or by aerosol. using two inoculations of virus, at 5 and 26 days of age, protective immunity was induced in chickens which resisted challenge with a pathogenic fowlpox virus given either via the wing web or intravenously at 46 days of age. aerosol and wing web vaccination induced slightly better protective immunity than drinking water or oral vaccination. virus was detected in lung cells of chicks vaccinated by ... | 1990 | 18679975 |
| outbreak of atypical fowlpox in chickens with persistent reticuloendotheliosis viraemia. | during the autumn in s/eastern australia, eight chickens of 7 months of age with an experimentally-induced reticuloendotheliosis virus (rev) viraemia developed cutaneous manifestations similar to fowlpox. ten hatchmates housed in a separate pen nearby, and known to be free of rev infection, remained clinically normal. fowlpox virus was isolated from the affected birds and histologically the lesions were characteristic of fowlpox. unsuccessful application of fowlpox vaccine by wing web 'stab', 2 ... | 1987 | 18766601 |
| fowlpox virus: its structural proteins and immunogens and the detection of viral-specific antibodies by elisa. | this study showed a possible 30 structural polypeptides in the fowlpox virion. there were three major structural proteins, of relative molecular mass (rmm) 91, 64, and 58 kda which were not major immunogens. although most viral polypeptides were shown to be immunogenic, antibodies in chicken sera taken after a primary fowlpox inoculation reacted strongly only with polypeptides of 37 and 35 kda, whereas antibodies in sera taken after a secondary inoculation also reacted strongly with six other vi ... | 1987 | 18766637 |
| integration of the reticuloendotheliosis virus envelope gene into the poultry fowlpox virus genome is not universal. | fowlpox virus (fwpv) is found worldwide in poultry and wild birds. fwpv is a natural example of recombination between viruses, as reticuloendotheliosis virus (rev) fragments have been found in all poultry fwpvs and these are implicated in virulence alteration. we aimed to determine the commonality of this phenomenon and analysed fwpvs collected from 128 poultry flocks and birds over the last 10 years. various fragments of both viruses were amplified and sequenced at the fwpv integration site, lo ... | 2008 | 18796713 |
| [construction of recombinant fowlpox virus coexpressing ha from subtype h5 of avian influenza virus and chicken interleukin-18]. | we developed recombinant fowlpox viruses (rfpv) coexpressing chicken il-18 and h5 aiv ha. | 2008 | 18956750 |
| recombinant fowlpox virus vector-based vaccine completely protects chickens from h5n1 avian influenza virus. | with the widespread presence of influenza virus h5n1 in poultry and wildlife species, particularly migrating birds, vaccination has become an important control strategy for avian influenza (ai). in this study, the immune efficacy and hemagglutination inhibition (hi) antibody responses induced by a recombinant fowlpox virus (fpv) vector-based rfpv-ha-na vaccine was evaluated in spf and commercial chickens. four-week old spf chickens vaccinated with one dose of vaccine containing 2 x 10(3) plaque ... | 2009 | 19110002 |
| re-emerging fowlpox: evaluation of isolates from vaccinated flocks. | vaccines of fowlpox or pigeonpox virus origin have been routinely used for more than half a century to prevent fowlpox in commercial poultry in areas where the disease is endemic. however, in recent years, outbreaks of fowlpox have occurred in previously vaccinated flocks. one possible explanation for this problem is the emergence of variant strains of fowlpox virus (fpv). a second, not mutually exclusive, postulate is that the novel fpv exhibit enhanced virulence due to the integration of avian ... | 2000 | 19184837 |
| protection of chickens from infectious laryngotracheitis with a recombinant fowlpox virus expressing glycoprotein b of infectious laryngotracheitis virus. | infectious laryngotracheitis (ilt) is an economically important disease of chickens caused by a type i gallid herpesvirus, infectious laryngotracheitis virus (iltv). the vaccines currently available are modified live viruses, which are effective in preventing disease outbreaks. however, they have often been associated with a variety of adverse effects including spread of vaccine virus to non-vaccinates, inadequate attenuation, production of latently infected carriers, and increased virulence as ... | 2001 | 19184888 |
| detection and quantitation of fowl adenovirus genome by a real-time pcr assay. | the purpose of the study was to develop a highly sensitive real-time polymerase chain reaction (pcr) assay to detect and quantitate fowl adenovirus (fadv) dna in chicken tissues, using fadv-9 as a model. the assay had a dynamic range of 7 logs and minimum detection limit of 9.4 viral genome copies. it was shown to be highly specific, as tissues from uninfected chickens and other viral genomes, such as those of marek's disease virus, fowlpox virus and infectious laryngotracheitis virus did not pr ... | 2009 | 19442846 |
| [anti-tumor effects on human laryngeal carcinoma hep-2 of recombinant fowlpox virus expressing chicken anemia virus apoptin gene]. | to investigate the anti-tumor effects and the mechanism of the recombinant fowlpox virus expressing apoptin gene on human laryngeal carcinoma hep-2. | 2009 | 19621600 |
| molecular biological characterization of avian poxvirus strains isolated from different avian species. | fifteen strains of avipoxvirus from different avian species were isolated and molecular biologically characterized. most strains did not produce evident pocks on the chorioallantoic membranes of commercial and specific-pathogen free embryonated chicken eggs where, on the contrary, microscopic signs of viral growth were always detected. polymerase chain reaction of highly conserved p4b gene was positive for all cases confirming to be a reliable diagnostic method for avipoxvirus. sequencing of the ... | 2010 | 19646826 |
| protective immune response of chickens to oral vaccination with thermostable live fowlpox virus vaccine (strain tpv-1) coated on oiled rice. | the objective of the present study was to develop and evaluate a local vaccine (strain tpv-1) against fowl pox (fp) in chickens. two separate groups of chickens were vaccinated with fp vaccine through oral (coated on oiled rice) and wing web stab routes, respectively. the results showed that the haemagglutination-inhibition (hi) antibody titres in both vaccinated groups were comparable and significantly higher (p < 0.05) than the control chickens. it was further revealed that 14 days after vacci ... | 2010 | 19714476 |
| protection of chickens against infectious bronchitis by a recombinant fowlpox virus co-expressing ibv-s1 and chicken ifngamma. | a fowlpox virus expressing the chicken infectious bronchitis virus (ibv) s1 gene of the lx4 strain (rfpv-ibvs1) and a fowlpox virus co-expressing the s1 gene and the chicken type ii interferon gene (rfpv-ibvs1-chifngamma) were constructed. these viruses were assessed for their immunological efficacy on specific-pathogen-free (spf) chickens challenged with a virulent ibv. although the antibody levels in the rfpv-ibvs1-chifngamma-vaccinated group were lower than those in the attenuated live ib vac ... | 2009 | 19786144 |
| characterization of host responses against a recombinant fowlpox virus-vectored vaccine expressing the hemagglutinin antigen of an avian influenza virus. | there currently are commercial fowlpox virus (fpv)-vectored vaccines for use in chickens, including trovac-aiv h5, which expresses the hemagglutinin (ha) antigen of an avian influenza virus and can confer immunity against avian influenza in chickens. despite the use of recombinant fpv (rfpv) for vaccine delivery, very little is known about the immune responses generated by these viruses in chickens. the present study was designed to investigate host responses to rfpv in vivo and in vitro. in cul ... | 2010 | 20071494 |
| [construction of recombinant fowlpox virus coexpressing ha gene from h5n1 avian influenza virus and chicken interleukin-2 gene and assessment of its protective efficacy]. | the hemagglutinin (ha) gene from h5n1 avian influenza virus and the chicken interleukin 2 (chiil-2) gene were inserted into a expressing vector p12ls to construct a recombinant transferring vector p12lsh5ail2, in which ha gene under the control of the promoter ps was in inverse tandem connection with the chiil-2 gene under the control of the promoter pe/l. the p12lsh5ail2 was then used to transfect the chicken embryo fibroblasts (cef) pre-infected with a wild-type fowlpox virus 282e4 strain, to ... | 2009 | 20077933 |
| severe histiolymphocytic and heterophilic bronchopneumonia as a reaction to in ovo fowlpox vaccination in broiler chicks. | broiler chickens on several farms from a single poultry company experienced neurological signs and mortality in chicks between 3 days and 10 days of age over a 3-week period after use of a fowlpox-vectored infectious laryngotracheitis virus vaccine in ovo. at necropsy the lungs contained numerous tan or gray, opaque to translucent, 0.5- to 2.0-mm nodules in the parenchyma. microscopic lesions were a multifocal severe lymphohistiocytic and heterophilic bronchopneumonia. immunohistochemistry was p ... | 2010 | 20080499 |
| diagnosis of a naturally occurring dual infection of layer chickens with fowlpox virus and gallid herpesvirus 1 (infectious laryngotracheitis virus). | an outbreak of acute respiratory disease in layers was diagnosed as being of dual nature due to fowlpox and infectious laryngotracheitis using a multidisciplinary approach including virus isolation, histopathology, electron microscopy and polymerase chain reaction (pcr). the diagnosis was based on virus isolation of gallid herpesvirus 1 (gahv-1) in chicken kidney cells and fowlpox virus (fwpv) in 9-day-old chicken embryonated eggs inoculated via the chorioallantoic membrane. the histopathology o ... | 2010 | 20390533 |
| a safety assessment of a fowlpox-vectored mycoplasma gallisepticum vaccine in chickens. | a recombinant fowlpox virus vaccine expressing key protective mycoplasma gallisepticum antigens could facilitate in the prevention both of fowlpox virus and m. gallisepticum infections. vectormune fp-mg vaccine, a recombinant fowlpox virus expressing both m. gallisepticum 40k and mgc genes, was assessed for its safety in 8-wk-old specific-pathogen-free white leghorn chickens. the vaccine virus was serially passaged 5 times by wing-web inoculation. based on the postinoculation clinical observatio ... | 2010 | 20460677 |
| [induction of potent immune responses by recombinant fowlpox virus with deleted orf73 or orf214]. | to determine if the fowlpox virus (fpv) orf73 or orf214 gene encoding protein has the function of il-18 binding protein, and to assess the role that orf73 or orf214 gene in regulating the immune response. | 2010 | 20560355 |
| interaction of poxvirus intracellular mature virion proteins with the tpr domain of kinesin light chain in live infected cells revealed by two-photon-induced fluorescence resonance energy transfer fluorescence lifetime imaging microscopy. | using two-photon-induced fluorescence lifetime imaging microscopy, we corroborate an interaction (previously demonstrated by yeast two-hybrid domain analysis) of full-length vaccinia virus (vacv; an orthopoxvirus) a36 protein with the cellular microtubule motor protein kinesin. quenching of enhanced green fluorescent protein (egfp), fused to the c terminus of vacv a36, by monomeric red fluorescent protein (mdsred), fused to the tetratricopeptide repeat (tpr) domain of kinesin, was observed in li ... | 2010 | 20943972 |
| pathology and microbiology of dermal squamous cell carcinoma in young brown chickens reared on reused litter. | dermal squamous cell carcinoma (dscc) was found in young brown chicken flocks reared on reused litter in japan. dscc was often detected at slaughter from april 2007 to march 2009, especially in june and july 2007. no dscc was observed in the broiler chickens on the farms. twelve 11-wk-old brown chickens with dscc were investigated pathologically and microbiologically. various degrees of crater-like skin lesions were found on the back, waist, neck, legs, abdomen, and wings of the carcasses. the f ... | 2010 | 20945801 |
| construction and immunogenicity of a recombinant fowlpox vaccine coexpressing s1 glycoprotein of infectious bronchitis virus and chicken il-18. | infectious bronchitis virus (ibv) poses a major threat to the chicken industry worldwide. in this study, we developed a recombinant fowlpox virus (rfpv) vaccine expressing the ibv s1 gene and chicken interleukin-18 gene (il-18), rfpv-s1/il18. recombinant plasmid psy-s1/il18 was constructed by cloning chicken il-18 into fowlpox virus transfer plasmid containing s1 gene and transfected into the chicken embryo fibroblasts cell pre-infected with s-fpv-017 to generate rfpv-s1/il18. expression of the ... | 2010 | 20951182 |
| adjuvant activity of chicken interleukin-12 co-administered with infectious bursal disease virus recombinant vp2 antigen in chickens. | a recombinant fowlpox virus (rfpv/vp2) expressing infectious bursal diseases virus (ibdv) vp2 gene has been constructed. after purification and identification of rfpv/vp2, the adjuvant activity of the recombinant chicken il-12 (rchil-12), synthesized by our previous construct of rfpv/chil-12, in rfpv/vp2-expressed rvp2 antigen was assessed in one-week-old specific-pathogen free chickens. the results indicated that rchil-12 alone or rchil-12 plus mineral oil (mo) co-administered with rvp2 antigen ... | 2010 | 21035196 |
| evaluation of recombinant fowlpox virus expressing infectious bronchitis virus s1 gene and chicken interferon-γ gene for immune protection against heterologous strains. | a recombinant fowlpox virus (rfpv-ifnγs1) that co-expressed the infectious bronchitis virus (ibv) s1 gene and the chicken interferon-γ gene has been constructed. to evaluate the efficacy of the recombinant fowlpox virus vaccine against heterotypic ibv strains, 60 4-week-old specific-pathogen-free (spf) chickens were inoculated with this vaccine and 3 weeks post inoculation challenged with the homotypic ibv strain lx4 and the heterotypic ibv strains lhb, lhlj04xi, ltj95i and lsc99i. antibodies ag ... | 2011 | 21216313 |
| antigen-specific t-cell responses to a recombinant fowlpox virus are dependent on myd88 and interleukin-18 and independent of toll-like receptor 7 (tlr7)- and tlr9-mediated innate immune recognition. | fowlpox virus (fwpv) is a double-stranded dna virus long used as a live-attenuated vaccine against poultry diseases, but more recent interest has focused on its use as a mammalian vaccine vector. here, in a mouse model system using fwpv encoding the nominal target antigen chicken ovalbumin (ova) (fwpv(ova)), we describe for the first time some of the fundamental processes by which fwpv engages both the innate and adaptive immune systems. we show that toll-like receptor 7 (tlr7) and tlr9 are impo ... | 2011 | 21248035 |
| efficacy of avian influenza vaccine in poultry: a meta-analysis. | vaccination is an effective method for controlling avian influenza (ai), especially in countries with endemic infection. this study conducted a bayesian meta-analysis to evaluate the efficacy of ai vaccines in chickens. we included both inactivated and recombinant fowlpox virus expressing h5 (rfpv-h5) vaccine studies that used specific-pathogen-free chickens where outcomes against the h5n1 or h5n2 ai viruses were measured. vaccine efficacy was evaluated by protection from mortality, protection f ... | 2010 | 21313840 |
| immunoadjuvant activities of a recombinant chicken il-12 in chickens vaccinated with newcastle disease virus recombinant hn protein. | recombinant fowlpox virus (rfpv/hn) expressing newcastle disease virus (ndv) hn gene and rfpv/hn/chil-12 co-expressing chicken il-12 (chil-12) and hn (rhn/chil-12) genes have been characterized. rhn/chil-12 or rchil-12, expressed by our previous construct rfpv/chil-12, co-administered with rhn was assessed for adjuvant activities of chil-12. chickens were vaccinated with various amounts of rhn/chil-12 mixed with mineral oil (mo), intramuscularly. levels of hemagglutination-inhibition (hi) antibo ... | 2011 | 21420255 |
| immune responses of chickens inoculated with a recombinant fowlpox vaccine coexpressing ha of h9n2 avain influenza virus and chicken il-18. | control of the circulation of h9n2 avian influenza virus (aiv) is a major concern for both animal and public health, and h9n2 aiv poses a major threat to the chicken industry worldwide. here, we developed a recombinant fowlpox virus (rfpv-ha) expressing the haemagglutinin (ha) gene of the a/ch/jy/1/05 (h9n2) influenza virus and a recombinant fowlpox virus (rfpv-ha/il18) expressing the ha gene and chicken interleukin-18 (il-18) gene. recombinant plasmid psy-ha/il18 was constructed by cloning chic ... | 2011 | 21549153 |
| gene expression profiling in rmd5- and rmd5deltameq-infected chickens. | marek's disease (md) is a lymphoproliferative disorder of domestic chickens caused by a highly contagious and oncogenic alpha-herpesvirus, marek's disease virus (mdv). md is characterized by bursal-thymic atrophy and rapid onset of t-cell lymphomas that infiltrate lymphoid tissues, visceral organs, and peripheral nerves with severe clinical signs that include transient paralysis, anemia, weight loss, and neurologic disorders. using overlapping cosmids- and bac-cloned mdv, it has been shown that ... | 2011 | 22017031 |
| outbreak of cutaneous form of poxvirus on a commercial turkey farm caused by the species fowlpox. | the present report documents the occurrence of a poxvirus infection in commercial meat turkeys. the affected farm had six flocks, with a total of 11,680 birds at different ages; birds from two of these flocks were affected. the clinical picture was characterized by severe epithelial lesions and proliferations on the head and neck regions as reported for the cutaneous form of poxvirus infection. except for these lesions, no adverse clinical signs or gross pathologic lesions were observed. only a ... | 2011 | 22312999 |
| [detection of fowlpox in chickens and turkeys in germany]. | the present work outlines molecular diagnostic examinations for detection of poxvirus infection in chickens and turkeys in germany over a time period of twelve years. diagnostic samples suspected for fowlpox were investigated using the polymerase chain reaction (pcr) in combination with restriction enzyme analysis (rea) for presence of fowlpox virus (fpv) specific dna. for a long period of time fowlpox did not play a role in commercial poultry farms in germany. beginning in 1999 an increasing nu ... | 2012 | 22372326 |
| the immune response of a recombinant fowlpox virus coexpressing the ha gene of the h5n1 highly pathogenic avian influenza virus and chicken interleukin 6 gene in ducks. | ducks have played an important role in the emergence of h5n1 subtype of highly pathogenic avian influenza (hpai), and the development of an effective vaccine against hpai in ducks is a top priority. it has been shown that a recombinant fowlpox virus (fpv)-vectored vaccine can provide protection against hpai in ducks. in this study, a recombinant fowlpox virus (rfpv-aih5ail6) coexpressing the haemagglutinin (ha) gene of the h5n1 subtype of the avian influenza virus (aiv) and chicken interleukin 6 ... | 2012 | 22902682 |
| development of an antigen-capture elisa for the detection of avian leukosis virus p27 antigen. | an antigen-capture enzyme-linked immunosorbent assay (ac-elisa) employing monoclonal and polyclonal antibodies against p27 was developed for the detection of the avian leukosis virus (alv). the specificity of the optimized ac-elisa was evaluated using avian leukosis virus subgroup j (alv-j), avian leukosis virus subgroup a (alv-a), avian leukosis virus subgroup b (alv-b), avian infectious bronchitis virus (ibv), marek's disease virus (mdv), avian infectious laryngotracheitis virus (iltv), fowlpo ... | 2013 | 23201286 |
| recombinant duck enteritis virus works as a single-dose vaccine in broilers providing rapid protection against h5n1 influenza infection. | although vaccination is an important strategy for controlling h5n1 avian influenza virus infections, broilers (short-lived meat chickens) remain the major victims in disease-endemic countries. inactivated vaccine usually requires 2-3weeks to establish solid protection, and recombinant vaccines, including the recombinant fowlpox virus vaccine and the recombinant newcastle disease virus vaccine are affected by maternal antibodies against the vectors. these disadvantages compromise the protective e ... | 2012 | 23267833 |
| genetic screen of a library of chimeric poxviruses identifies an ankyrin repeat protein involved in resistance to the avian type i interferon response. | viruses must be able to resist host innate responses, especially the type i interferon (ifn) response. they do so by preventing the induction or activity of ifn and/or by resisting the antiviral effectors that it induces. poxviruses are no exception, with many mechanisms identified whereby mammalian poxviruses, notably, vaccinia virus (vacv), but also cowpox and myxoma viruses, are able to evade host ifn responses. similar mechanisms have not been described for avian poxviruses (avipoxviruses). ... | 2013 | 23427151 |
| genetic screen of a mutant poxvirus library identifies an ankyrin repeat protein involved in blocking induction of avian type i interferon. | mammalian poxviruses, including vaccinia virus (vacv), have evolved multiple mechanisms to evade the host type i interferon (ifn) responses at different levels, with viral proteins targeting ifn induction, signaling, and antiviral effector functions. avian poxviruses (avipoxviruses), which have been developed as recombinant vaccine vectors for permissive (i.e., poultry) and nonpermissive (i.e., mammals, including humans) species, encode no obvious equivalents of any of these proteins. we show th ... | 2013 | 23427153 |
| the peptide motif of the single dominantly expressed class i molecule of the chicken mhc can explain the response to a molecular defined vaccine of infectious bursal disease virus (ibdv). | in contrast to typical mammals, the chicken mhc (the bf-bl region of the b locus) has strong genetic associations with resistance and susceptibility to infectious pathogens as well as responses to vaccines. we have shown that the chicken mhc encodes a single dominantly expressed class i molecule whose peptide-binding motifs can determine resistance to viral pathogens, such as rous sarcoma virus and marek's disease virus. in this report, we examine the response to a molecular defined vaccine, fp- ... | 2013 | 23644721 |
| protection against h7n3 high pathogenicity avian influenza in chickens immunized with a recombinant fowlpox and an inactivated avian influenza vaccines. | beginning on june 2012, an h7n3 highly pathogenic avian influenza (hpai) epizootic was reported in the state of jalisco (mexico), with some 22.4 million chickens that died, were slaughtered on affected farms or were preemptively culled on neighboring farms. in the current study, layer chickens were vaccinated with a recombinant fowlpox virus vaccine containing a low pathogenic ai (lpai) h7 gene insert (rfpv-h7-aiv) and an inactivated oil-emulsified h7n3 aiv vaccine, and subsequently challenged a ... | 2013 | 23707445 |
| an outbreak of lymphomas in a layer chicken flock previously infected with fowlpox virus containing integrated reticuloendotheliosis virus. | visceral lymphomas occurred in a 236-day-old layer flock previously diagnosed with reticuloendotheliosis virus (rev)-integrated fowlpox virus (fpv) infection at the age of 77 days. common pathologic lesions were multiple neoplastic nodules of homogeneous lymphocytes in the livers and spleens of all submitted chickens. all neoplastic tissues were positive for the rev envelope (env) gene by pcr. in a retrospective molecular study of fpv-infected 77-day-old chickens from the same flock, we identifi ... | 2013 | 24597128 |
| newcastle disease virus (ndv) recombinants expressing infectious laryngotracheitis virus (iltv) glycoproteins gb and gd protect chickens against iltv and ndv challenges. | infectious laryngotracheitis (ilt) is a highly contagious acute respiratory disease of chickens caused by infectious laryngotracheitis virus (iltv). the disease is controlled mainly through biosecurity and vaccination with live attenuated strains of iltv and vectored vaccines based on turkey herpesvirus (hvt) and fowlpox virus (fpv). the current live attenuated vaccines (chicken embryo origin [ceo] and tissue culture origin [tco]), although effective, can regain virulence, whereas hvt- and fpv-v ... | 2014 | 24829337 |
| highly pathogenic fowlpox virus in cutaneously infected chickens, china. | we investigated an acute outbreak of the cutaneous form of fowlpox among chickens in china in november 2009. using pathologic and virologic methods, we identified a novel type of fowlpox virus that carried an integrated genomic sequence of reticuloendotheliosis virus. this highly pathogenic virus could lead to severe ecologic effects and economic losses. | 2014 | 24963887 |
| differential diagnosis of fowlpox and infectious laryngotracheitis viruses in chicken diphtheritic manifestations by mono and duplex real-time polymerase chain reaction. | infectious laryngotracheitis virus (iltv) and fowlpox virus (fpv) cause diphtheritic lesions in chicken tracheas and can simultaneously infect the same bird. a differential molecular diagnostic test, the duplex real-time polymerase chain reaction, is now reported using iltv and fpv vaccine viruses and clinical samples from chickens, either uninfected or naturally infected with iltv or fpv, or with both viruses. the dual virus amplification by real-time polymerase chain reaction was demonstrated ... | 2015 | 25317604 |
| immunogenicity and efficacy of fowlpox-vectored and inactivated avian influenza vaccines alone or in a prime-boost schedule in chickens with maternal antibodies. | inactivated and fowlpox virus (fp)-vectored vaccines have been used to control h5 avian influenza (ai) in poultry. in h5 ai endemic countries, breeder flocks are vaccinated and therefore, maternally-derived antibodies (mda) are transferred to their progeny. results of three immunogenicity and one efficacy studies performed in birds with or without mda indicated that the immunogenicity of an inactivated vaccine based on a h5n9 ai isolate (inh5n9) was severely impaired in chicks hatched from inh5n ... | 2014 | 25359591 |
| detection of t- and b-cell target antigens of fowlpox virus isolated from backyard chickens in india. | with the aim of assessing the antigenic characteristics of a circulating pool of fowlpox virus (fpv) that exists in the backyard poultry system in india, one of the field isolates generated was characterized by in vitro immunologic techniques. fpv was isolated from clinically positive fowlpox cases (n = 10) from the jhargram (west midnapur district) and kakdwip (south 24 pargana district) areas of west bengal state, india. initially, fpv-specific pcr was performed for confirmation of the sampl ... | 2015 | 26473675 |
| evaluation of the protection efficacy of a serotype 1 marek's disease virus-vectored bivalent vaccine against infectious laryngotracheitis and marek's disease. | laryngotracheitis (lt) is a highly contagious respiratory disease of chickens that produces significant economic losses to the poultry industry. traditionally, lt has been controlled by administration of modified live vaccines. in recent years, the use of recombinant dna-derived vaccines using turkey herpesvirus (hvt) and fowlpox virus has expanded, as they protect not only against the vector used but also against lt. however, hvt-based vaccines confer limited protection against challenge, with ... | 2015 | 26473676 |
| vaccination of 1-day-old turkey poults with fowlpox vaccine by subcutaneous route. | in high-challenge areas, 1-day-old chicks are often vaccinated with fowlpox vaccine to provide early protection against the disease. however, we were unable to find any information in the published literature on the safety and efficacy of live fowlpox virus vaccine in 1-day-old turkeys. in this study, we evaluated a freeze-dried chicken-embryo-origin live fowlpox virus vaccine for its safety and efficacy in 1-day-old turkey poults by administering the vaccine subcutaneously in the upper back of ... | 2015 | 26478161 |
| emergence of reticuloendotheliosis virus in pigeons in guangdong province, southern china. | reticuloendotheliosis virus (rev), an important immunosuppressive pathogen, has many hosts, including chickens, ducks, geese, turkeys, and wild birds. clinically, rev may lead to increased susceptibility to other pathogens, resulting in serious tissue damage (especially tumors) and the death of its host. in this study, we encountered a disease outbreak resulting in a large number of deaths of pigeons in guangdong province, southern china. histopathological analysis revealed apparent tumor-like l ... | 2016 | 27120185 |
| concurrent fowlpox and candidiasis diseases in backyard chickens with unusual pox lesions in the bursa of fabricius. | concurrent fowlpox and candidiasis diseases occurred in a backyard chicken flock. four deceased chickens (one nagoya breed and three white silkie chickens) were examined for diagnosis. at necropsy, white curd-like plaques were observed in the crop. fungal elements that stained positive for candida albicans with immunohistochemistry were distributed throughout the tongue, choanal mucosa, esophagus, and crop. typical fowlpox lesions, composed of proliferating epithelial cells with ballooning degen ... | 2016 | 27610735 |
| identification of clade e avipoxvirus, mozambique, 2016. | analysis of scab samples collected from poultry during outbreaks of fowlpox in mozambique in 2016 revealed the presence of clade e avipoxviruses. infected poultry were from flocks that had been vaccinated against fowlpox virus. these findings require urgent reevaluation of the vaccine formula and control strategies in this country. | 2017 | 28820373 |