Publications

spontaneous spinal plexiform perineurioma in a juvenile chicken ( gallus domesticus).	a 2-mo-old pet chicken ( gallus domesticus) was presented because of lameness and a hind limb mass of 1 mo duration. radiographs revealed a soft tissue mass extending from the mid-femur into the body wall. cytology of a sample obtained from a fine-needle aspirate was unremarkable. the bird was prepared for surgical investigation of the mass but died acutely at the time of pre-medication. autopsy revealed an extradural mesenchymal neoplasm that focally compressed the lumbar spinal cord, extended ...	2017	28381111
domestic chickens activate a pirna defense against avian leukosis virus.	piwi-interacting rnas (pirnas) protect the germ line by targeting transposable elements (tes) through the base-pair complementarity. we do not know how pirnas co-evolve with tes in chickens. here we reported that all active tes in the chicken germ line are targeted by pirnas, and as tes lose their activity, the corresponding pirnas erode away. we observed de novo pirna birth as host responds to a recent retroviral invasion. avian leukosis virus (alv) has endogenized prior to chicken domesticatio ...	2017	28384097
integrated host and viral transcriptome analyses reveal pathology and inflammatory response mechanisms to alv-j injection in spf chickens.	avian leukosis virus (alv) is detrimental to poultry health and causes substantial economic losses from mortality and decreased performance. because tumorigenesis is a complex mechanism, the regulatory architecture of the immune system is likely to include the added dimensions of modulation by mirnas and long-noncoding rna (lncrna). to characterize the response to alv challenge, we developed a novel methodology that combines four datasets: mrna expression and the associated regulatory factors of ...	2017	28401895
circular rna alterations are involved in resistance to avian leukosis virus subgroup-j-induced tumor formation in chickens.	avian leukosis virus subgroup (alv-j) is an oncogenic neoplasm-inducing retrovirus that causes significant economic losses in the poultry industry. recent studies have demonstrated circular rnas (circrnas) are implicated in pathogenic processes; however, no research has indicated circrnas are involved in resistance to disease. in this study, over 1800 circrnas were detected by circrna sequencing of liver tissues from alv-j-resistant (n = 3) and alv-j-susceptible chickens (n = 3). 32 differential ...	2017	28415618
development of a taqman-based real-time pcr assay for rapid and specific detection of fowl aviadenovirus serotype 4.	twelve serotypes of fowl aviadenovirus, namely, fadv-(1-8a and 8b-11), have been identified, among which fadv-4 is the aetiologic agent of hepatitis hydropericardium syndrome (hhs) in chickens. outbreaks of hhs have been documented in many countries, causing significant economic losses. real-time pcr methods described so far in the literature cross-detect different serotypes of fadvs. in this study, we aimed to develop a taqman-based real-time pcr assay for the specific detection of fadv-4. a pa ...	2017	28437156
molecular characterization, expression of chicken tbk1 gene and its effect on irf3 signaling pathway.	traf family member-associated nf-κb activator (tank)-binding kinase1 (tbk1) is a serine-threonine kinase at the crossroads of multiple interferon (ifn)-inducing signaling pathways in innate immunity. the importance of tbk1 in antiviral immunity is well established in mammal models, but in chicken, the molecular characterization and potential function of tbk1 remain unclear. in the present study, the open-reading frame (orf) of chicken tbk1 (chtbk1) was cloned and characterized. the sequencing re ...	2017	28493975
a novel avian retrovirus associated with lymphocytoma isolated from a local chinese flock induced significantly reduced growth and immune suppression in spf chickens.	avian leukosis viruses (alvs) are associated with neoplasias, immune suppression and reduced performance in chicken flocks. in the present study, a naturally occurring recombinant strain of alv (fj15ht0) was isolated from an infected flock of chinese "hetian" chickens, and was subsequently identified as an exogenous alv by immuno-fluorescence assay (ifa), pcr and following entire proviral dna nucleotide sequencing. this isolate is revealed as a novel recombinant virus, lacking viral oncogenes, w ...	2017	28622858
vertical transmission of avian leukosis virus subgroup j (alv-j) from hens infected through artificial insemination with alv-j infected semen.	avian leukosis virus (alv) is one of the main causes of tumour development within the poultry industry in china. the subgroup j avian leukosis viruses (alv-j), which induce erythroblastosis and myelocytomatosis, have the greatest pathogenicity and transmission ability within this class of viruses. alv can be transmitted both horizontally and vertically; however, the effects of alv infection in chickens-especially roosters-during the propagation, on future generations is not clear. knowing the ro ...	2017	28662658
discovery of novel long non-coding rnas induced by subgroup j avian leukosis virus infection in chicken.	avian leukosis virus subgroup j (alv-j) is an avian oncogenic retrovirus that has led to severe economic losses in the poultry industry in china in recent decades. here, using high throughput transcriptome sequencing of hd11 and cef cells infected with alv-j, a set of 4804 novel long non-coding transcripts and numerous differentially expressed long non-coding rnas (lncrnas) were identified. we also found that they share relatively shorter transcripts and fewer exon numbers compared to mrna. corr ...	2017	28673822
dynamic co-evolution and interaction of avian leukosis virus genetic variants and host immune responses.	subgroup j avian leukosis virus (alv-j), a typical retrovirus, is characterized of existence of a cloud of diverse variants and considerable genetic diversity. previous studies describing the evolutionary dynamics of alv-j genetic variants mainly focused on the early infection period or few randomly selected clones. here, we inoculated 30 specific-pathogen-free chickens with the same founder alv-j stock of known genetic background. six (three antibody positive and three antibody negative) chicke ...	2017	28694798
full-length genome sequence analysis of four subgroup j avian leukosis virus strains isolated from chickens with clinical hemangioma.	since 2014, cases of hemangioma associated with avian leukosis virus subgroup j (alv-j) have been emerging in commercial chickens in guangxi. in this study, four strains of the subgroup j avian leukosis virus (alv-j), named gx14hg01, gx14hg04, gx14lt07, and gx14zs14, were isolated from chickens with clinical hemangioma in 2014 by df-1 cell culture and then identified with elisa detection of alv group specific antigen p27, the detection of subtype specific pcr and indirect immunofluorescence assa ...	2017	28718045
complete genome sequencing and characterization revealed a recombinant subgroup b isolate of avian leukosis virus with a subgroup j-like u3 region.	one natural recombinant subgroup b avian leukosis virus (alv) with a subgroup j-like u3 region was isolated from commercial native chickens that experienced disease in 2014 and named gx14ff03. gx14ff03 was isolated by df-1 cell culture and then identified with elisa detection of avian leukosis virus p27 group-specific antigen, the detection of subtype specific pcr, and indirect immunofluorescence assay with alv-b-specific monoclonal antibody. its complete proviral genome was sequenced and compar ...	2017	28718046
16s rrna genes illumina sequencing revealed differential cecal microbiome in specific pathogen free chickens infected with different subgroup of avian leukosis viruses.	intestinal flora play important roles in the pathogenisis of many pathogens. this study examined the cecal microbiome of chickens infected with avian leukosis virus (alv) using 16s rrna genes illumina sequencing. one-day-old specific pathogen free chicks were inoculated in the abdomen with subgroup j or k of alv. at 21-day-old, chickens positive for alv viremia were selected and their cecal contents were extracted and examined for the composition of gut microflora by illumina sequencing of the v ...	2017	28757024