Publications

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on the gametogonic cycle of toxoplasma gondii. 19715162518
isolation of toxoplasma gondii from guinea pigs in singapore. 19715168251
life cycle of toxoplasma gondii. 19695359949
observations on the faecal transmission of toxoplasma gondii. 19695377776
the new cyst of toxoplasma gondii. 19695383714
[experimental toxoplasma infections in cats. i. repeated transmission of toxoplasma gondii through feces of cats infected by nematodes]. 19695393352
coccidian-like nature of toxoplasma gondii.specific pathogen-free domestic cats were fed with tissue cysts containing toxoplasma gondii. in two infected cats large numbers of oocysts were produced in the faeces; no oocysts were observed in the faeces of the uninfected control cat. five days after the feeding of the toxoplasms profuse schizogonic and gametogonic stages were observed in the epithelial cells of the small intestine of one infected cat. a single schizont was observed in an intestinal epithelial cell of a second cat six days a ...19705413951
toxoplasma gondii: the oocyst, sporozoite, and infection of cultured cells.the infective form of toxoplasma gondii fouind in cat feces is an oocyst wihich, when sporutlated, resembles that of the genus isospora in havinig two sporocysts. sporozoites obtained by artificial excystation of the oocyst are infective for monkey kidney cell cultures. ultrastructural characteristics of sporozoites resemble those seen previously in proliferative stages of toxoplasma gondii.19705460694
characterization of the new fecal form of toxoplasma gondii. 19705467864
toxoplasma gondii life cycle in cats. 19705530378
use of the microprecipitation method in agar gel (mpa) in the diagnostics of toxoplasmosis. ii. correlation of mpa and the sft (sabin-feldman dye test) with results of toxoplasma gondii isolation experiments. 19705531288
[endopolygeny in toxoplasma gondii]. 19715555447
experimental toxoplasmosis in chimpanzees.two chimpanzees were given by mouth large numbers of viable oocysts of toxoplasma gondii obtained from the faeces of experimentally infected cats. before the experiment the first chimpanzee had a positive dye test reaction (1:250), an indication that it had undergone an earlier infection of toxoplasmosis; the serum antibody titres remained unchanged, no evidence of illness was found, and oocysts did not appear in its faeces during the subsequent six weeks. the second chimpanzee showed a negative ...19715575975
isolation of toxoplasma gondii from the feces of a helminth free cat. 19685719075
the faecal transmission of toxoplasma gondii. 19685721204
[tests on the transmission of toxoplasma gondii by nematodes in domestic animals]. 19685752817
toxoplasma gondii: transmission through feces in absence of toxocara cati eggs.when incubated at room temperature (23 degrees c) for 3 to 14 days, feces from cats infected 4 to 8 days with toxoplasma gondii, and free of toxocara cati eggs, produced toxoplasmosis in mice. results indicate that the nematode egg is not necessary for transmission of the parasite.19695813385
toxoplasma gondii: fecal forms separated from eggs of the nematode toxocara cati.cats excreted the protozoan toxoplasma gondii in feces, generally between 5 to 12 days after ingesting mice with chronic toxoplasmosis. toxoplasma gondii and eggs of the nematode toxocara cati occurring together could be separated by washing them through sieves that retained the eggs. this finding negates the postulated role of toxocara cati in the transmission of toxoplasmosis.19695813386
experimental transmission of toxoplasma gondii. 19655839865
[infection of the cat, per os, by the vegetative and cystic forms of toxoplasma gondii]. 19655840744
[epidemiological studies on toxoplasmosis. isolation of strains of toxoplasma gondii from domestic animals]. 19645890780
toxoplasma gondii from toxocara cati. 19665945168
the nematode transmission of toxoplasma gondii. 19676031940
distribution of toxoplasma gondii in the tissues of infected cats. i. isolation in mice. 19676054492
distribution of toxoplasma gondii in the tissues of cats. ii. histopathological study. 19676054493
toxoplasma gondii: genetic crosses reveal phenotypic suppression of hydroxyurea resistance by fluorodeoxyuridine resistance.mutants resistant to sinefungin (sf) and hydroxyurea (hu) were isolated from an oocyst-producing strain of toxoplasma gondii with the aid of mutagenesis with ethylnitrosourea. these mutants were used with previously described mutants resistant to adenine arabinoside (araa) and fluorodeoxyuridine (fudr) in genetic crosses in cats. in order to interpret the data from crosses in which all four mutants were used to infect the same cat, it was necessary to devise a mathematical expression to predict ...19836219892
protozoan and viral infections of feral cats.identification of protozoan oocysts and serological tests were used to determine the prevalence of infections among 300 mainly adult feral cats in three different habitat types in south-eastern australia. oocysts of isospora rivolta and isospora felis were recovered from 3% and 4% respectively of 300 feral cat samples. haemagglutination inhibition antibody to toxoplasma gondii was detected in 20% of 75 cat sera tested. a high prevalence of specific antibody to feline panleukopaenia virus (79%) a ...19816280665
observations on some epidemiological aspects of toxoplasmosis in malaysia.a review of the various studies on toxoplasmosis in peninsular malaysia is presented. the period of review spanned between 1973 and 1980 during which a number of serological surveys were carried out for the presence of toxoplasma gondii antibody in malaysians, using either the indirect hemagglutination (i.h.a.) or the indirect fluorescent antibody (i.f.a.) tests. the prevalence rates of toxoplasma antibody were consistently foundhighest among malays, followed by indians, orang aslis (aborigines) ...19846389404
besnoitia darlingi (apicomplexa, sarcocystidae, toxoplasmatinae): transmission between opossums and cats.opossums (didelphis marsupialis), act as intermediate hosts for besnoitia darlingi and could be infected orally with sporozoites (oocysts) and bradyzoites (tissue cysts), or intraperitoneally (i.p.) with tachyzoites. infections could presumably be transmitted through cannibalism. cats (felis catus), the definitive host, could be infected only with bradyzoites but not sporozoites. oocysts shed by cats measure about 12 x 12 microns, resemble similarly sized oocysts of toxoplasma gondii and hammond ...19846439859
pathogenesis of acute toxoplasmosis in specific-pathogen-free cats.systemic toxoplasmosis was produced in specific-pathogen-free cats by intravenous inoculation with toxoplasma gondii tachyzoites. infectious organisms were recovered from all tissues studied, but the number of organisms recovered from liver, lungs and spleen was 10-fold to 10,000-fold higher than from heart and brain. the occurrence and severity of toxoplasma-induced lesions correlated with the number of infectious organisms recovered from the various tissues. in nonlymphoid tissues, the toxopla ...19816457439
immunizing efficacy of toxoplasma gondii strains isolated from different hosts.studies were carried out to determine the fate of virulent toxoplasma gondii challenge in immune animals. toxoplasma strains isolated from human, swine, rabbit and cat hosts were used for the primary immunization of mice. brains were removed at various intervals after the challenge and subinoculated into normal mice. prior immunization with one of the nine toxoplasma strains enabled the mice either to eliminate or harbour the challenge organisms without loss of virulence. the outcome of challeng ...19846499407
the prevalence of public health significance of toxoplasma gondii in domestic cats in the niger delta. 19846522573
toxoplasmosis infection associated with eating undercooked venison.toxoplasmosis infection was documented in one alabama and two south carolina deer hunters in 1980. all three cases were acutely ill and required hospitalization. physical findings included fever, leukopenia, and abnormal liver function tests. two of the three patients showed infiltrates on chest x-ray. all three hunters had recently consumed raw or nearly raw venison and lacked other known risk factors for toxoplasmosis such as cat contact or eating other undercooked meats. in a serosurvey and q ...19836650484
toxoplasma gondii--an environmental contaminant.toxoplasma gondii, the causal agent of toxoplasmosis, is a ubiquitous protozoan parasite which has the potential to infect man and all warm-blooded animals. it has as its definitive host the cat. infections acquired by the human adult are generally subclinical. however, if acquired for the first time during pregnancy, the parasite can invade the developing foetus. under these circumstances infection can result in abortion, stillbirth or production of live-born offspring with severe multiple orga ...19836678762
experimental infection of a bison with toxoplasma gondii oocysts. 19836684185
distribution of cysts and tachyzoites in calves and pregnant cows inoculated with toxoplasma gondii oocysts.sixteen calves and 6 cows were each inoculated with 100 000 infective oocysts of the gt-1 strain of toxoplasma gondii. cattle were necropsied between 3 and 287 days post-inoculation (dpi) and their tissues were inoculated into mice or fed to toxoplasma-free cats for the detection to toxoplasma in bovine tissues. ten to 10 000-fold more t. gondii were recovered from small intestine and mesenteric lymph nodes of calves at 3 and 6 dpi than from lungs and liver, and the number of t. gondii in bovine ...19836686377
feline bronchial cytology: histologic/cytologic correlation in 22 cats.trichrome-stained bronchial washings were obtained from 22 cats at necropsy and compared to lung tissue sections obtained at the same time. in three of the 22 cats, antemortem bronchial washings also were obtained. sixteen of the cats were clinically ill and six were clinically normal and served as controls. on the basis of comparison of histologic sections and bronchial washings, patterns were detected in the washings indicative of general processes such as alveolar edema, chronic passive conge ...19846730221
pediatric infectious cervical lymphadenitis.infectious cervical lymphadenitis is a frequently encountered problem in the pediatric population. the recent experience with infectious cervical lymphadenitis at the children's hospital of philadelphia was reviewed. the most common etiologic agent was staphylococcus aureus. beta-hemolytic streptococcus and mycobacterium were found in 15% and 6% of cases respectively. other agents included pneumococcus, cat scratch, toxoplasma gondii, actinomycosis, and mixed bacteroides and peptostreptococcus. ...19806821410
[isolation of toxoplasma gondii oocysts in 2 districts of recife (pe)]. 19836879049
animal toxoplasmosis in iran.a latex agglutination slide test for toxoplasma antibodies was used on serum samples collected from various animals (111 cats, 113 dogs, three jackals, 393 sheep, 272 goats, 69 cows) in different parts of iran. the sero-positive rate in titres 1:2-1:256 in different animals ranged from 12.6 to 56.0%; the highest (56.0%) was found in the stray dogs. suspensions of the brains of 120 sero-positive animals (17 cats, 14 dogs, one jackal, 66 sheep, 22 goats) as well as 13 sero-negative animals (11 dog ...19836887318
prevalence of toxoplasma gondii antibodies and oocysts in pound-source cats.the overall prevalence of serum antibodies to toxoplasma gondii in 87 pound-source cats from the state of washington was 31%; its was 41% for stray cats and 28% for owner-relinquished cats. approximately 60% of the 27 seropositive cats had dye-test titers greater than or equal to 1:256. oocysts of toxoplasma gondii were not observed in fecal specimens from 73 of the cats. it was concluded that the high antibody titers, indicative of recently acquired infection, could result in increased risk to ...19827096176
immunization of cats against shedding of toxoplasma oocysts.development of immunity to the shedding of oocysts was examined in 75 kittens that survived infection with the three stages of toxoplasma gondii. of 16 kittens fed bradyzoites in cysts, 94% were immune and did not shed oocysts. of seven injected with tachyzoites 86% were immune. of 18 fed sporozoites only 11% were immune, but following injection, 54% of 12 were immune. after the administration of either bradyzoites or tachyzoites from nonoocyst-producing strains, only 9% of 22 were immune. consi ...19827131181
prevalence of toxoplasma gondii antibodies in pet dogs and cats. 19827138437
isolation of encysted toxoplasma gondii from muscles of mule deer in montana.toxoplasma gondii was isolated from muscles of 2 road-killed mule deer found near bozeman, mont, by feeding muscles to a toxoplasma-free cat. the cat shed t gondii oocysts 5 days after ingesting muscles pooled from the 2 deer. one infective oocyst of this isolate of t gondii was lethal to mice.19827161172
induced toxoplasma gondii, toxocara canis, and isospora canis infections in coyotes.six littermate laboratory-raised coyotes (no. 1-6) were used to study the transmission of toxoplasma gondii from cats and mice, and toxocara canis and isospora canis from dogs and mice. coyotes 1 and 2 were inoculated orally with 1,000 infective oocysts of the tc-1 strain of t gondii; coyotes 3 and 4 were fed mice infected with tissue cysts of the tc-1 strain of t gondii. eight days after inoculation with t gondii, the same 4 coyotes were exposed to toxocara canis: coyotes 1 and 2 were inoculate ...19827174440
epizootic toxoplasmosis associated with abortion in dairy goats in montana.perinatal toxoplasmosis was diagnosed in goats from 2 farms in montana. of 7 pregnant does from one farm, 1 aborted a dead fetus and 2 each had a kid infected in utero. toxoplasma gondii was demonstrated histologically in the placenta of 2 of the infected does and by mouse inoculation in all 3 of them. the organism also was isolated from internal organs of 6 of 7 does, 4 of 4 cats, and 3 of 11 chickens from the farm. a pregnant doe from the other farm delivered 3 kids infected with t gondii. one ...19817204243
[prevalence of toxoplasma gondii infection in domestic and wild animals, and human groups of the amazonas region]. 19807231162
characterization of toxoplasma gondii isolates from an outbreak of toxoplasmosis in atlanta, georgia.toxoplasma gondii was isolated from tissue of 5 of 7 cats and of 4 of 4 field mice trapped in a riding stable in atlanta where an outbreak of toxoplasmosis had occurred in persons. dye-test antibodies were not found in serum of 4 cats and 4 field mice shown to be infected with t gondii by mouse inoculation. the pathogenicity and infectivity of the 9 isolates of t gondii from cats and mice were compared in mice with the same characteristics of an isolate from a person who acquired toxoplasmosis i ...19817283229
an in vitro study on the toxoplasmacidal activity of lonomycin a in host cells.lonomycin a at various concentrations was tested for its inhibitory effect on toxoplasma multiplication in host cells cultured in vitro. results indicate that lonomycin a at a concentration of 0.01 micrograms per ml in tc-199 medium demonstrated a high degree of antitoxoplasma activity with complete inhibition of toxoplasma multiplication in the host cells. lymphokines, a supernatant produced from spleen cells of mice infected chronically with toxoplasma gondii, inhibited toxoplasma multiplicati ...19817298512
sex determination and sex differentiation in isospora (toxoplasma) gondii.although sexual differentiation in the life-cycle of eimeriina (coccidia) becomes morphologically evident during development of the gamonts, there are indications in the literature that pregametogonic schizonts might already be sexually differentiated. on the other hand development of both sexes from cloned postzygotic, presumably haploid stages has been reported. in the present experiments we cloned successive stages of the coccidian representative isopora (toxoplasma) gondii to determine the t ...19817324138
toxoplasmosis update and public health implications.toxoplasma gondii has a coccidian life cycle in the intestine of domestic and wild felids that includes a series of asexual and sexual stages and an oocyst stage that is shed in the feces. oocysts complete their development outside the body, eventually becoming infective for about 350 species of vertebrates including cats and man. the effects of climate on oocyst survival and the physical and biological means of oocyst dispersal are discussed. infectivity and pathogenicity for livestock species ...19817337909
toxoplasma gondii isolated from amniotic fluid.a pregnant woman contracted toxoplasmosis from exposure to oocysts shed by cats. she underwent amniocentesis for a therapeutic abortion, and toxoplasma gondii was isolated from the amniotic fluid and placenta. this method may be useful in determining whether the fetus is infected in cases of toxoplasmosis acquired during pregnancy.19807360443
experimental toxoplasmosis in calves and pregnant cows.experimental toxoplasma gondii infections were studied in pregnant cows and in calves. in tests to compare their virulence, three strains of the toxoplasmal parasite were red to cats; then fecal oocysts were collected and given per os to calves. in tests to determine their effects, virulent tachyzoites or oocysts were given to 10 calves and to 22 pregnant cows by the oral, iv, or intraamniotic routes. clinical signs were fever and inappetence. one cow in early gestation aborted 24 days after iv ...19807362114
experimental toxoplasmosis in elk (cervus canadensis).one cow elk (approx 4 months pregnant) and one calf elk were each inoculated intraruminally with 10(5) infective oocysts of toxoplasma gondii. both animals were seronegative when inoculated, but became seropositive to t gondii. sabin-feldman dye test antibody titers in relation to day postinoculation were as follows: 1:1024, 1:1024, and 1:2048 (days 28, 69, and 73) for the calf; 1:2048, 1:128, and 1:128 (days 34, 69, and 73) for the cow; and 1:2546 (73 days after inoculation of the cow) for the ...19807406299
prevalence of toxoplasma gondii antibodies in the chinese population of hong kong.sera from 2,499 randomly selected chinese inhabitants of hong kong were tested for the presence of toxoplasma gondii antibodies using the indirect fluorescent antibody test. the over-all prevalence was 9.8%. the prevalence was significantly higher in the 10 to 19 and 30 to 39 year age group and the highest level of titres was found in the 20 to 29 group. there was no significant difference in prevalence between the male and female population and between the rural and city dwellers. the distinctl ...19807434429
toxoplasma gondii in costa rican cats.toxoplasma gondii infection of cats was studied in seven localities in costa rica. a total of 237 cats were tested for antibody by the dye test, and for oocysts by injection into mice. toxoplasma was isolated from 55 (23%) and antibody was found in 109 (46%). sixty percent of cats were infected as shown by shedding or antibody. of the 55 isolations, 64% were from kittens without antibody and 36% from kittens with antibody. almost half of the toxoplasma isolations were made from the feces of kitt ...19807446806
intermediate and transport hosts of toxoplasma gondii in costa rica.toxoplasma gondii was isolated from 3.5% of 202 mus musculus, 12.5% of 120 rattus norvegicus, 16% of 106 sparrows (zonotrichia capensis), and from 54% of 50 chickens from costa rica. the infection rate in chickens increased from 23% in those weighing less than 500 g, to 73% in those between 500 and 1,000 g, indicating a rapid rate of acquiring infectin, probably from the soil in which they constantly search for food. no isolates were made from musca domestica, or from four genera of roaches caug ...19807446807
human toxoplasmosis and cat contact in costa rica.we studied the prevalence of antibody to toxoplasma gondii in 883 costa ricans between the ages of 15 and 26 years (mean 20.3). there were seven populations, two rural and five urban. the overall antibody prevalence was 61.4%. because antibody prevalence was 57.1% in the 14 people who ate raw meat, and 61.6% in the 294 people who consumed raw eggs, meat and eggs could be excluded as important modes of transmission. we ascertained the presence of cats, type of soil contact, and flooring in the ki ...19807446808
scanning electron microscopy of the cat small intestine and mucosal alteration observed during toxoplasma gondii infection. 19807458458
sources and reservoirs of toxoplasma gondii infection on 47 swine farms in illinois.field studies were conducted on 47 swine farms in illinois during 1992 and 1993 to identify sources and reservoirs of toxoplasma gondii infection. blood samples were obtained from swine and from trapped wildlife. serum antibodies to t. gondii were determined using the modified agglutination test, incorporating mercaptoethanol. antibodies to t. gondii (titer > or = 25) were found in 97 of 4,252 (2.3%) finishing pigs, 395 of 2,617 (15.1%) sows, 267 of 391 (68.3%) cats, 126 of 188 (67.0%) raccoons, ...19957472862
risk factors for transmission of toxoplasma gondii on swine farms in illinois.two epidemiologic studies of risk factors for transmission of toxoplasma gondii to swine were conducted for farms in illinois. the first study was a cross-sectional survey of swine farms from the state of illinois pseudorabies testing program, in which farm owners or managers were interviewed by telephone regarding presence of risk factors for transmission of t. gondii on the farm. there were 123 farms surveyed that provided blood samples for at least 30 sows. the mean sow seroprevalence was 19. ...19957472865
hammondia hammondi cysts in cell cultures.in an attempt to obtain the continuous development of hammondia hammondi in vitro, culture of the parasite was performed in 3 cell lines. although organisms were present in culture fluids of feline kidney cells (crfk) for as long as 3 mo, continuous culture was not possible. however, for the first time, cysts of h. hammondi were observed in cell culture from day 6 after inoculation of sporozoites. ultrastructure of the h. hammondi cysts was similar to that observed for in vitro-obtained toxoplas ...19957472890
[epidemiology of toxoplasma gondii infection in the municipality of ribeirâo das neves, mg. i. importance of domestic animals as sources of t. gondii infection in humans].during a six month period (june 1983 to january 1984), a case-control study was carried out to determine if the variables associated with domestic animals contact would be correlated with the prevalence of toxoplasma gondii infection. a randomized sample of 500 subjects from the population of ribeirão das neves, minas gerais, brazil was studied. a statistically significant correlation was found with cat, chicken and pig contact but none with consumption of meat, milk and eggs.19957480915
cockroaches as transport hosts of the protozoan toxoplasma gondii. 19947480943
human toxoplasma infection in kuna and embera children in the bayano and san blas, eastern panama.we conducted a survey of 760 amerindian children 2-12 years of age in the bayano and san blas areas of panama in 1991 to determine the prevalence of serum antibodies to toxoplasma gondii and the importance of hypothesized risk factors in human-induced native and sylvatic conditions, which have had few environmental changes, as opposed to rural and urban areas in panama previously studied. the overall prevalence of infection ranged between 0% and 42.5%. no age curve was detected, indicative of no ...19957485702
transmission of toxoplasma gondii in panama city, panama: a five-year prospective cohort study of children, cats, rodents, birds, and soil.a cohort of more than 500 children from panama city, panama was studied prospectively over five years for acquisition of antibody to toxoplasma gondii. the direct agglutination test showed that 72 of 571 children seroconverted between one and six years of age, for a cumulative incidence of 12.6%. children were examined by pediatricians quarterly, and illnesses that had occurred in the interval and their activities were noted on questionnaires. thirty-eight variables were examined for their role ...19957485703
comparison of methods for estimation of toxoplasma gondii-specific antibody production in the aqueous humor of cats.intraocular production of toxoplasma gondii-specific antibody in cats has been estimated by comparing the ratio of t gondii-specific antibody in aqueous humor and serum with the ratio of total immunoglobulins in serum and aqueous humor (goldmann-witmer coefficient; aqueous antibody coefficient; c value). it has been proposed that in human beings, comparison of the ratio of t gondii-specific antibody in aqueous humor and serum with the ratio of antibodies against a nonocular pathogen in serum and ...19957486396
[animal foci and transmission routes of toxoplasma gondii].in the transmission of t. gondii from animal sources to man a major part is played by animal tissues containing toxoplasmatic cysts and toxoplasmatic oocysts which result from the sexual reproduction of the parasites in the small intestine of feline animals. tachyzoits play a minor role in the development of toxoplasmosis in man, most frequently when animals, their tissues and secretions are handled in the stage of parasitaemia. as to domestic animals, rabbits and sheep are infected most frequen ...19957489135
[prevalence of toxoplasmosis in pregnant women of the province of la habana].a seroepidemiological study of toxoplasma gondii was carried out in four municipalities of havana province from october 1990 to april 1991 using a 10 microl ultra micro-elisa. we tested 362 serum samples, from pregnant women, and 71% of toxoplasmic infection was found. toxoplasmic infection was more frequent in women living in rural zones having domestic contacts with cats. the relationship of toxoplasmic infection and spontaneous abortion antecedent in this group was analyzed but no statistical ...19947569612
prevalence of toxoplasma gondii antibodies in cats in the western part of great buenos aires, argentina, 1993.the serological prevalence of toxoplasma gondii was determined, using indirect haemagglutination assay, in the sera of 169 cats. classification by age, sex, alimentary, hunting and roaming habits was made in conjunction with the number of cats living in each house. an important prevalence was detected (19.5%) from 1 year of age and statistical significance was found in those animals that had predatory habits or lived in groups.19957571341
diagnosis of induced toxoplasmosis in neonatal cats.sixteen pregnant queens were inoculated orally with tissue cysts of toxoplasma gondii, and fetal membranes and offspring were examined for t gondii infection by bioassay in mice. queens appeared clinically normal, although all shed t gondii oocysts. toxoplasma gondii was isolated from tissues of 7 of 33 fetuses or kittens from 5 litters (at 13, 23, 26, 27, and 29 postinoculation days) from 8 queens euthanatized between 10 and 31 postinoculation days. infection with t gondii was found in kittens ...19957601711
detection of toxoplasma gondii-specific iga in the serum of cats.an elisa for detection of toxoplasma gondii-specific iga in feline serum was developed. a group of cats (n = 7) was inoculated orally with t gondii bradyzoites. toxoplasma gondii-specific serum igm, igg, and iga responses were followed sequentially by use of the elisa for 34 weeks. serum iga was detected later than igm or igg, and was detected in most cats on week 34 after inoculation. none of the cats was seropositive for iga during the oocyst-shedding period. a group of client-owned cats with ...19957653886
detection of toxoplasma gondii-specific iga in the aqueous humor of cats.toxoplasma gondii-specific iga, igm, and igg were measured by elisa in the serum and aqueous humor of 29 client-owned cats with endogenous uveitis and 7 specific-pathogen-free cats tested sequentially for 20 weeks after inoculation with t gondii. local antibody production in aqueous humor was estimated by multiplying the aqueous humor-to-serum t gondii-specific antibody ratio by the serum-to-aqueous humor total igg (c value) or calicivirus-specific igg (ctc value) ratio. evidence for local produ ...19957653887
risk factors associated with transmission of toxoplasma gondii to sows kept in different management systems in tennessee.of 3841 serum samples from sows received from the tennessee state diagnostic laboratory in 1991-1992, 1130 were positive for toxoplasma gondii antibody. the total number of farms sampled was 343. the observed seroprevalence was 36% for both years. a questionnaire that dealt with herd management practices was mailed to 303 of the swine farms that were sampled; 107 (35%) responses were returned and analyzed. there was no significant difference in the seroprevalence of t. gondii in the sows on the ...19957660566
[parasitology and human medical preventive importance of toxoplasma gondii].present knowledge of the biology and distribution of toxoplasma gondii allows to provide recommendations for primary prevention of infection with the parasite. the recommendations are chiefly designed for "seronegative" pregnant women without specific serum anti-t.-gondii-igg and for persons with continuous or temporary immune deficiencies. prevention should focus on 3 main sources of infection risk: meat: meat should only be eaten when well cooked or when it has been frozen prior to preparation ...19957716446
[toxoplasma gondii: perspectives for a vaccine].to date no single vaccine has been commercialized in the field of human parasitology, and therefore a practical approach to a potential toxoplasma vaccine in the field can only be discussed theoretically. the aim of such a vaccine would consist either in inhibiting endogenous parasite multiplication (tachyzoite formation) and thus dissemination, or in preventing the final formation of toxoplasma cysts (bradyzoite formation). immune protectivity should confer resistance to disease and parasite di ...19957716458
antibody prevalence of toxoplasmosis in arabian gazelles and oryx in saudi arabia.we determined the antibody prevalence of toxoplasmosis (toxoplasma gondii infections) in antelopes in saudi arabia. serum samples from 324 gazella subgutturosa marica, 202 gazella gazella, 70 gazella dorcas, and 12 arabian oryx (oryx leucoryx) were tested by the indirect hemagglutination test. antibodies to t. gondii were detected in 13(4.0%) g. subgutturosa marica, 12(5.9%) g. gazella, 3(4.3%) g. dorcas, and in none of the arabian oryx sampled. more adult gazelles were seropositive than juvenil ...19947760490
duration of immunity to shedding of toxoplasma gondii oocysts by cats.cats that have shed toxoplasma gondii oocysts are considered to be immune to reshedding of oocysts. to investigate if this immunity persists in cats for 6 yr, 12 4-6-mo-old cats without t. gondii antibodies were inoculated orally with tissue cysts of the me-49 strain (6 cats) and the ts-2 strain (6 cats) of t. gondii. all of them shed > or = 20 million oocysts between 4 and 13 days after feeding tissue cysts. two cats became ill between 11 and 13 days after primary infection; 1 died on the 13th ...19957776126
prevalence of antibodies to toxoplasma gondii in army dogs in the czech republic and slovak republic.army dogs, mostly german sheep-dogs, were tested for antibodies to t. gondii using sabin-feldman dye test (dt) and complement fixation test (cft). titers greater than or equal to 4 and 10 were considered as positive in dt and cft, respectively. seroprevalences in the group of 720 dogs from the civil sector of different areas in the former czechoslovakia were 39.3% and 15.4% in dt and cft, respectively. during dogs' stay in army seroprevalences decreased only slowly. the decrease was probably due ...19957793014
safety and efficacy of modified live feline toxoplasma gondii vaccine. 19957796964
phylogenetic relationship of sarcocystis neurona to other members of the family sarcocystidae based on small subunit ribosomal rna gene sequence.sarcocystis neurona is a coccidial parasite that causes a neurologic disease of horses in north and south america. the natural host species are not known and classification is based on ultrastructural analysis. the small subunit ribosomal rna (ssurna) gene of s. neurona was amplified using polymerase chain reaction techniques and sequenced by sanger sequencing reactions. the sequence was compared with partial sequences of s. muris, s. gigantea, s. tenella, s. cruzi, s. arieticanis, s. capracanis ...19947799170
toxoplasma gondii-specific antibodies in the aqueous humor of cats with toxoplasmosis.we decided to determine whether toxoplasma gondii-specific igm or igg is produced locally or is deposited in the aqueous humor of t gondii-naive cats after primary or secondary inoculation with t gondii. cats were orally inoculated with t gondii tissue cysts during weeks 0 and 36. aqueous humor and serum t gondii-specific igm and igg were measured, using elisa, during weeks 0, 2, 4, 6, 8, 12, 16, 20, 26, 34, 38, 40, 42, 44, 48, 56, 62, 66, and 72 after primary oral inoculation. total immunoglobu ...19947802391
tissue cyst formation of toxoplasma gondii t-263 in cell culture. 19947804222
safety aspects of a vaccine for cats containing a toxoplasma gondii mutant strain. 19947804276
the effect of toxoplasma gondii and other parasites on activity levels in wild and hybrid rattus norvegicus.using both correlational and experimental evidence, the relationship between parasite load and host activity was assessed in brown rats, rattus norvegicus. two hypotheses were tested--(1) that parasites with indirect life-cycles, involving transmission between a prey and its predator, will alter the activity of the intermediate host so as to increase its susceptibility to predation by the definitive host and (2) that activity levels in parasitized rats would be increased rather than decreased. f ...19947831094
congenital toxoplasmosis among premature infants with different clinical pictures in saudi arabia.no doubt, toxoplasmosis is a prevalent infection in all the arab countries. however, the incidence differs from one country to another and even within the same country. congenital toxoplasmosis is the most important form of the infection. in the present study, some 78 premature infants with different clinical pictures were examined for toxoplasma igg (by ihat and elisa) and toxoplasma igm (by elisa). the results showed toxoplasma igg ranging between 32.1% (ihat) and 46.2% (elisa) while igm (elis ...19947844430
prevalence of viable toxoplasma gondii tissue cysts and antibodies to t. gondii by various serologic tests in black bears (ursus americanus) from pennsylvania.during the hunting season of 1993, hearts from 28 black bears (ursus americanus) from pennsylvania were examined for toxoplasma gondii infection. serum samples from heart blood were examined for t. gondii antibodies by the use of the modified agglutination test (mat), the sabin-feldman dye test (dt), the latex agglutination test (lat), and the indirect hemagglutination test (iha). one-hundred grams of myocardium from each bear were bioassayed in mice. approximately 500 g of myocardium from 11 be ...19957876963
toxoplasma gondii in iowa sows: comparison of antibody titers to isolation of t. gondii by bioassays in mice and cats.hearts of 1,000 pigs killed at an abattoir in iowa were bioassayed for the prevalence of tissue cysts of toxoplasma gondii. one hundred grams of cardiac muscle from each pig was homogenized, digested in pepsin solution, and bioassayed in 10 mice. five hundred grams of heart tissue from each of a subset of 183 pigs was also bioassayed in cats. serum collected from the heart from each pig was assayed for anti-t. gondii antibodies in the modified agglutination test using formalin-fixed whole tachyz ...19957876977
fatal toxoplasmosis in five cats.fatal toxoplasmosis was diagnosed in five of 155 cats necropsied in denmark during a 2 year period, thus representing 3.2% of the necropsied animals. lesions were dominated by scattered necrotic foci in liver and spleen. toxoplasma gondii was demonstrated primarily by an immunoperoxidase technique. the clinical signs were rhinitis, conjunctivitis, dyspnoea and neurological malfunction. the relatively high prevalence indicates that clinical and fatal toxoplasmosis, naturally acquired, seems more ...19947886915
effects of incidental infections and immune activation on disease progression in experimentally feline immunodeficiency virus-infected cats.specific pathogen-free cats were experimentally infected with feline immunodeficiency virus (fiv) and subsequently exposed to common infectious pathogens and immune stimuli over a 3-year period. cats with preexisting fiv infection showed signs of disease after exposure to haemobartonella felis, toxoplasma gondii, feline herpesvirus-1, and feline calicivirus similar to signs in non-fiv-infected cats, although they were more severe. no adverse effects of immunization with inactivated rabies virus ...19947916048
killing of different strains of toxoplasma gondii tissue cysts by irradiation under defined conditions.to study the effect of gamma-irradiation on the viability of toxoplasma gondii tissue cysts, brains of mice inoculated with 95 newly isolated strains of t. gondii from pigs and 10 other laboratory isolates were pooled, flattened, packed in vacuum, and irradiated to absorbed doses of 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, and 0.9 kgy at 5 c (+/- 0.5 c) with a 137cs gamma-radiation source. treated samples were bioassayed for viable t. gondii in mice, cats, or both. tissue cysts of all strains were ren ...19947931910
evaluation of a direct agglutination test for detection of antibodies against toxoplasma gondii in cat, pig and sheep sera. 19947942387
characterization of toxoplasma and trichinella isolates from muscles of black bears in pennsylvania.during the hunting season of 1992, 322 black bears from pennsylvania were examined for toxoplasma gondii- and trichinella spp-induced infections. toxoplasma gondii antibodies were found in 79.8% of 322 bears--titer < 1:25 in 65 (20.2%), 1:25 in 18 (5.6%), 1:50 in 11 (34.5%) and 1:500 in 128 (38.7%) bears--by use of the modified agglutination test. muscle tissues from 89 of these bears were bioassayed for t gondii parasites. muscles from 64 bears, including heart from 1 bear, and heart alone from ...19947944020
prevalence and transmission of toxoplasma gondii in wild brown rats, rattus norvegicus.prevalence of toxoplasma gondii within 6 uk farmstead wild rat populations, and 1 population maintained within a captive cat-free environment for 2.5 years, was examined. the mean prevalence found was 35% (n = 235), which is more than 3 times as high as all other wild rat prevalence levels previously reported in the uk. there were no significant age, sex or site differences in prevalence between or within populations irrespective of habitat type or presence of cats. toxoplasma was also maintaine ...19948008454
serologic survey and serum biochemical reference ranges of the free-ranging mountain lion (felis concolor) in california.serum samples from 58 mountain lions (felis concolor) in california (usa) were collected between april 1987 and february 1990. nineteen serum samples were used for serum biochemistry determinations; the ranges were similar to reference values in domestic cats, captive exotic felidae and free-ranging mountain lions. a serological survey was conducted to determine whether antibodies were present against selected infectious agents. fifty-four (93%) of 58 sera had antibodies against feline panleukop ...19948028105
elisas based on recombinant antigens for seroepidemiological studies on toxoplasma gondii infections in cats.two recombinant toxoplasma gondii polypeptides, h4 and h11, were tested as diagnostic antigens in enzyme-linked immunosorbent assays (elisas). the results obtained by elisas based on single h4 (h4-elisa), on single h11 (h11-elisa) and on a mixture of h4 and h11 (h4/h11-elisa) were compared with results obtained by an elisa based on traditional elisa antigen (tea-elisa), an indirect fluorescent antibody test (ifat), the sabin-feldman dye test (sfdt) and a direct agglutination test (dat). a total ...19948058366
effect of toxoplasma gondii upon neophobic behaviour in wild brown rats, rattus norvegicus.the effect of toxoplasma gondii on neophobic behaviour (the avoidance of novel stimuli) was assessed in four groups of wild rats with naturally occurring toxoplasma infection. two groups were placed in individual cages and tested in a series of experiments which examined the effect of toxoplasma on the rat's reaction to 3 food-related novel stimuli (odour, food-container, food). a trappability study was performed on the other two groups to test whether toxoplasma had an effect on probability of ...19948058367
epidemiologic observations on infection with rochalimaea species among cats living in baltimore, md.cats from several sources in baltimore, md, were tested for seropositivity to rochalimaea henselae and r quintana. co-infection with toxoplasma gondii or feline immunodeficiency virus was assessed as a risk factor for infection with rochalimaea spp. of 592 cats tested, 87 (14.7%) were seropositive for one or both rochalimaea spp, although titers to r henselae were significantly higher than those to r quintana. prevalence of seropositivity increased significantly with cat age and weight and was a ...19948063598
transfer of antibodies to kittens from mother cats chronically infected with toxoplasma gondii.by indirect immunofluorescence assay, anti-toxoplasma gondii antibody levels were examined in fetuses and kittens born from chronically infected cats. titer of anti-t. gondii igg in sera of kittens born from infected cats was significantly high on the seventh day post-birth, and decreased to a serologically non-detectable level at 8-12 weeks post-birth under continuous suckling of maternal milk. littermates nursed by a non-infected cat showed a faster rate of igg antibody depletion. in sera of f ...19948073605
toxoplasmosis and mental retardation--report of a case-control study.a case-control study evaluating the association between mental retardation and toxoplasmosis was conducted among 845 school children in belo horizonte, mg, brazil. cases (450) were mentally retarded children attending a public school for special education. controls (395) were children from the regular public school system. clinical and anthropometric examinations and interviews were carried out to determine risk factors for toxoplasmosis and mental retardation. diagnosis of toxoplasma gondii inf ...19938107587
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