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oocysts ingestion as an important transmission route of toxoplasma gondii in brazilian urban children.abstract toxoplasmosis is a cosmopolitan protozoan infection. data regarding risk factors for the post-natal acquisition of toxoplasma gondii infection in childhood are limited. we conducted a serological survey for t. gondii igg antibodies and associated risk factors in 1,217, 4- to 11-yr-old children from salvador, brazil using a commercial elisa kit; antibodies were found in 17.5% of the children. age (or=2.18; 95%ci: 1.50-3.17) and maternal schooling level (or=0.62; 95%ci: 0.42-0.92) were ne ...201121740247
results of parasitological examinations of faecal samples from cats and dogs in germany between 2003 and 2010.in a retrospective study, the results of parasitological examinations of faecal samples from 8,560 cats and 24,677 dogs between january 2003 and december 2010 in germany were analysed. 30.4 % of the examined dogs and 22.8 % of the cats were infected with endoparasites. the examination of the faecal samples from dogs revealed stages of giardia spp. (18.6 %), toxocara canis (6.1 %), toxascaris leonina (0.6 %), ancylostomatidae (2.2 %), trichuris vulpis (1.2 %), capillaria spp. (1.3 %), crenosoma v ...201121739375
genetic characterization of toxoplasma gondii isolates from pigs in southwestern china.abstract the genetic diversity of toxoplasma gondii varies in different geographical regions. isolates of t. gondii in south america, for example, are genetically and biologically divergent from those in north america and europe, where the population structure is highly clonal and composed mainly of three distinct lineages known as types i, ii, and iii. however, little is known of the t. gondii genotypes in people's republic of china. toxoplasma gondii infection in pigs causes significant econom ...201121721904
lack of awareness of risk factors for primary toxoplasmosis in pregnancy.background: the overall seroprevalence of toxoplasma antibodies in women of childbearing age in ireland is 25% [1]. hence, 75% of women remain susceptible to primary toxoplasma infection during pregnancy, which if transmitted to the foetus can cause ocular, neurological and other sequelae. toxoplasma exposure during pregnancy can be avoided if there is an awareness of the potential sources of infection, mainly contaminated food, water, soil and cat faeces. aims: to determine risk factor exposure ...201121660651
ovine and caprine toxoplasmosis (toxoplasma gondii) in aborted animals in jordanian goat and sheep flocks.two hundred and fifty-five biological samples (106 aborted foetal tissue samples and 149 blood samples from aborted sheep and goats) were collected from 188 animals during the lambing season from september 2009 to april 2010 from the mafraq region of jordan. the sampled animals belonged to 93 goat and sheep flocks that had cases of abortion. a total of 169 (66.3%) biological samples were collected from sheep and 86 (33.7%) from goats. seventy-six (29.8%) biological samples (45 blood and 31 tissu ...201121643666
flowcytomix analysis for toxoplasma gondii infection in pregnant women in central taiwan.summary the study was to determine the seroprevalence of toxoplasmosis in the sera of pregnant women in central taiwan and to investigate the levels of cytokine in the sera of pregnant women with toxoplasma gondii infection. the 220 blood samples were collected from pregnant women. the haematological parameters of peripheral blood were analysed by a haematology analyser. serum samples of the pregnant women were analysed by a commercially available anti-t. gondii igm/igg antibody enzyme-linked im ...201121627416
seroepidemiology of toxoplasma gondii, neospora caninum, and leishmania spp. infections and risk factors for cats from brazil.the seroprevalence of infection by toxoplasma gondii, neospora caninum, and leishmania spp. was detected through an indirect immunofluorescence in 70 cats from the andradina municipality, são paulo state, brazil. anti-t. gondii antibodies (titer >64) were detected in 15.7% (11/70) of animals, whereas positivity for n. caninum (titer 16) was not observed in any animal. of the cats from urban and rural areas, 10.4% (5/48) and 27.2% (6/22) were positive for t. gondii, respectively. breed, age, food ...201121626423
virulence differences in toxoplasma mediated by amplification of a family of polymorphic pseudokinases.the population structure of toxoplasma gondii includes three highly prevalent clonal lineages referred to as types i, ii, and iii, which differ greatly in virulence in the mouse model. previous studies have implicated a family of serine/threonine protein kinases found in rhoptries (rops) as important in mediating virulence differences between strain types. here, we explored the genetic basis of differences in virulence between the highly virulent type i lineage and moderately virulent type ii ba ...201121586633
prevalence of bartonella species, haemoplasmas and toxoplasma gondii in cats in scotland.the objective of this study was to determine the prevalence rates for select infectious agents of cats presented to the royal (dick) school of veterinary studies at the university of edinburgh, scotland. whole blood, serum, and oral mucosal and nail bed swabs were collected. while ehrlichia species, anaplasma species or rickettsia felis dna were not amplified from any cat, 44.2% of the cats had evidence of infection or exposure to either a bartonella species (15.3% were seropositive and 5.8% pol ...201121570883
sporulation and survival of toxoplasma gondii oocysts in different types of commercial cat litters.abstract toxoplasma gondii oocysts are environmentally resistant and can survive outdoors for months in dry and cold climates. in the present study, sporulation and survival of t. gondii oocysts was studied in different types of cat litters commercially available in the u.s. oocysts sporulated within 2-3 days in all types of bedding and occasionally remained viable for 14 days. results indicate that cat litter should be changed daily to prevent sporulation and infectivity to people.201121539466
prevalence of specific igg-antibodies against toxoplasma gondii in domestic turkeys determined by kinetic elisa based on recombinant gra7 and gra8.the protozoan parasite toxoplasma (t.) gondii is one of the most common zoonotic infectious agents worldwide. besides its sexual reproduction in cats, t. gondii can also infect a wide spectrum of other warm-blooded animals. these include animals used for human consumption such as pigs or chickens. nevertheless, the role of turkeys for the epidemiology of t. gondii infections has not been studied thoroughly. we have established a kinetic elisa (kela) for the detection of t. gondii-specific igg an ...201121524853
besnoitiosis in a southern plains woodrat (neotoma micropus) from uvalde, texas.abstract recently, besnoitia neotomofelis was described from a southern plains woodrat (neotoma micropus) from southern texas. during may 2010, 1 of 55 southern plains woodrats trapped in uvalde county, texas was diagnosed with besnoitiosis. grossly, the woodrat had bilateral swellings of the cheeks and numerous besnoitia sp.-like cysts were observed in the tongue, facial region, musculature of the limbs, and subcutis of the dorsum and flanks. little to no inflammation was noted around cysts. th ...201121524194
seroprevalence of toxoplasma gondii infection in feral raccoons (procyon lotor) in japan.abstract antibodies to toxoplasma gondii were found in 92 (9.9%) of 929 feral raccoons (procyon lotor) in japan using the latex agglutination test (lat, 1: 64 or higher). seropositivity varied by geographic location, season, weight, but by the sex of raccoons trapped. seroprevalences in the northern, central, and western areas of japan were found to be 7.9% (39/492), 16.5% (47/285), and 3.9% (6/152), respectively. the seroprevalence by season varied from 8.5% (13/153) in spring to 18.9% (14/74) ...201121510744
first description of naturally acquired tritrichomonas foetus infection in a persian cattery in spain.tritrichomonas foetus has been identified as the causative agent of feline intestinal trichomonosis, characterized by clinical signs of chronic large bowel diarrhoea. this disease has been reported in cats from the usa, europe and australia. however, its epidemiology is still unclear. the aim of the present study was to describe t. foetus infection in a persian cattery in spain. t. foetus infection was sequentially diagnosed in 20 cats by direct faecal smear examined under the microscope, specif ...201121509446
seroprevalence of toxoplasma gondii and concurrent bartonella spp., feline immunodeficiency virus, feline leukemia virus, and dirofilaria immitis infections in egyptian cats.abstract toxoplasma gondii and bartonella spp. are zoonotic pathogens of cats. feline immunodeficiency virus (fiv) and feline leukemia virus (felv) are related to human immunodeficiency virus and human leukemia virus, respectively, and these viruses are immunosuppressive. in the present study, the prevalence of antibodies to t. gondii , bartonella spp., fiv, as well as felv and dirofilaria immitis antigens was determined in sera from feral cats ( felis catus ) from cairo, egypt. using a modified ...201121506874
detection and differentiation of coccidian oocysts by real-time pcr and melting curve analysis.abstract rapid and reliable detection and identification of coccidian oocysts are essential for animal health and food-borne disease outbreak investigations. traditional microscopy and morphological techniques can identify large and unique oocysts, but are often subjective and require parasitological expertise. the objective of this study was to develop a real-time quantitative pcr (qpcr) assay with melting curve analysis (mca) to detect, differentiate, and identify dna from coccidian species of ...201121506835
identification of a sporozoite-specific antigen from toxoplasma gondii.abstract reduction of risk for human and food animal infection with toxoplasma gondii is hampered by the lack of epidemiological data documenting the predominant routes of infection (oocyst vs. tissue cyst consumption) in horizontally transmitted toxoplasmosis. existing serological assays can determine previous exposure to the parasite, but not the route of infection. we have used difference gel electrophoresis, in combination with tandem mass spectroscopy and western blot, to identify a sporozo ...201121506817
prevalence of antibodies to toxoplasma gondii in horses in riyadh province , saudi arabia.abstract the aim of the present study was to determine the seroprevalence of toxoplasma gondii in horses for sporting purposes in the province of riyadh, saudi arabia. in total, 266 serum samples from clinically healthy horses were analyzed for anti-t. gondii antibodies using the sabin-feldman dye test. antibodies to t. gondii were found in 84 (31.6%) horses, with specific titers of 1:16 (78 with a prevalence of 29.3%), 1:64 (4 with a prevalence of 1.5%), and 1:256 (2 with a prevalence of 0.8%). ...201121506811
isolation of viable toxoplasma gondii from feral guinea fowl (numida meleagris) and domestic rabbits (oryctolagus cuniculus) from brazil.abstract toxoplasma gondii was isolated from a feral guinea fowl (numida meleagris) and domestic rabbits (oryctologus cuniculus) from brazil for the first time. serum and brains from 10 guinea fowl and 21 rabbits from brazil were examined for t. gondii infection. antibodies to t. gondii were found in 2 of 10 fowl and 2 of 21 rabbits by the modified agglutination test (titer 1:25 or higher). viable t. gondii (designated tgnmbr1) was isolated from 1 of the 2 seropositive fowl by bioassay in mice, ...201121506805
seroprevalence and risk factors for toxoplasma gondii infection on pig farms in central china.abstract toxoplasma gondii is a protozoan parasite that causes severe diseases in mammals, including humans, around the world. in china, pork is the main meat source; accordingly, t. gondii in pigs is considered an important source for human toxoplasmosis. understanding the epidemiology of toxoplasmosis in pig farms is thus important for control of the disease in humans. the purpose of the present study was to investigate the epizootiology of t. gondii infections in pig farms in central china by ...201121506784
serological response of cats to experimental besnoitia darlingi and besnoitia neotomofelis infections and prevalence of antibodies to these parasites in cats from virginia and pennsylvania.abstract besnoitia darlingi and besnoitia neotomofelis are cyst-forming tissue apicomplexan parasites that use domestic cats ( felis domesticus ) as definitive hosts and opossums ( didelphis virginiana ) and southern plains woodrats ( neotoma micropus ) as intermediate hosts, respectively. nothing is known about the prevalence of b. darlingi or b. neotomofelis in cats from the united states. besnoitia darlingi infections have been reported in naturally infected opossums from many states in the u ...201121506782
occurrence and risk factors associated with infection by toxoplasma gondii in goats in the state of alagoas, brazil.goats are considered very susceptible to infection by toxoplasma gondii and when this occurs during pregnancy, it may cause fetal death with subsequent fetal resorption, abortion, mummification, stillborn or the birth of weak goats. the objective of this work was to determine the occurrence of and identify risk factors for t. gondii infection in goats in different mesoregions in the state of alagoas.201121503550
prevalence of antibody to toxoplasma gondii in terrestrial wildlife in a natural area.we conducted a cross-sectional study from 2008 to 2009 to evaluate the occurrence of feral and wild cats and the risk of toxoplasma gondii infection in terrestrial wildlife in a natural area in illinois, usa. felids are definitive hosts for t. gondii and cats are a key component of rural and urban transmission of t. gondii. we selected four forest sites within the interior of the park and four edge sites within 300 m of human buildings. feline and wildlife occurrence in the natural area was dete ...201121441191
medical microbiologytoxoplasma gondii is an intestinal coccidium that parasitizes members of the cat family as definitive hosts and has a wide range of intermediate hosts. infection is common in many warm-blooded animals, including humans. in most cases infection is asymptomatic, but devastating disease can occur.199621413265
prevalence of toxoplasma gondii infection in feral cats in seoul, korea.the present study assessed the prevalence of toxoplasma gondii infection in feral cat populations in seoul using enzyme-linked immunosorbent assay (elisa) and nested polymerase chain reaction (pcr). a total of 456 feral cats from 17 wards in seoul was surveyed. the overall prevalence of t. gondii infection was 15.8% (69/456) by elisa and 17.5% (80/456) by pcr; by gender, 17% (44/259) by elisa and 16.2% (42/259) by pcr in males and 14.3% (28/196) by elisa and 19.4% (38/196) by pcr in females. on ...201021348626
seroprevalence of toxoplasma gondii in cats from colombo, sri lanka.cats are essential in the life cycle of toxoplasma gondii because they are the only hosts that can excrete the environmentally resistant oocysts in nature. nothing is known of the prevalence of toxoplasma gondii in cats from sri lanka. serum samples from 86 cats from colombo, sri lanka, were tested for antibodies to t. gondii using the modified agglutination test; antibodies were found in 26 (30.2%) cats with titers of 1:25 in 4, 1:50 in 4, 1:100 in 3, 1:400 in 2, 1:800 in 3, 1:1,600 in 4, and 1 ...201021348625
toxoplasma gondii infection and schizophrenia: a case control study in a low toxoplasma seroprevalence mexican population.there are conflicting reports concerning the association of toxoplasma gondii infection and schizophrenia in humans. therefore, we determined such association in a mexican population of mestizo ethnicity. through a case-control study design, 50 schizophrenic patients and 150 control subjects matched by gender, age, residence place, and ethnicity were examined with enzyme-linked immunoassays for the presence and levels of t. gondii igg antibodies and for the presence of t. gondii igm antibodies. ...201121292026
prevalence of infectious diseases in cats and dogs rescued following hurricane katrina.to determine the prevalence of infectious diseases of animal and zoonotic importance in cats and dogs rescued and transferred from the gulf coast region following hurricane katrina.201121281213
immunological status against toxoplasma gondii in non-cat owners from an endemic region of mexico.a cross-sectional study was developed to determine anti-toxoplasma gondii immunoglobulin g (igg) and igm antibodies from 80 persons aged 18-21 years without a history of previous contact with cats. individuals who consented to take part in the survey were served with a questionnaire to obtain response on their eating habits. blood samples were taken and specific igm and igg antibodies against t. gondii were measured by indirect enzyme-linked immunoassay. seropositivity was found in 29 (37%) and ...201121254942
prevalence of igg antibodies to encephalitozoon cuniculi and toxoplasma gondii in cats with and without chronic kidney disease from virginia.kidney disease is a common and serious condition in domestic cats. there are numerous causes of kidney disease including parasitic infection. encephalitozoon cuniculi is a microsporidian parasite that develops in the kidneys of rabbits and causes chronic renal disease. little has been reported concerning e. cuniculi in cats and no serological studies on this parasite in cats have been conducted in the united states to date. the present study explored the possibility that e. cuniculi is an unreco ...201021247702
anti-toxoplasma gondii antibodies among volunteer blood donors in eastern saudi arabia.a cross-sectional study was conducted to evaluate the risk factors of toxoplasma gondii in 100 volunteer blood donors at blood bank. blood donors were interviewed about sociodemographic characteristics and risk factors toxoplasmosis. a required data were taken through a designed questionnaire. five ml. venous blood samples were withdrawn by disposable sterile syringes to separate sera. anti-toxoplasma antibodies were evaluated by using elisa. the results showed that 40 volunteers (40%) were posi ...201021246947
toxoplasmosis in a patient who was immunocompetent: a case report. 201121244658
isolation and genotyping of toxoplasma gondii causing fatal systemic toxoplasmosis in an immunocompetent 10-year-old cat.a 10-year-old male, neutered domestic shorthair cat was presented with fever, anorexia, vomiting, and diarrhea. serologic testing for feline immunodeficiency virus and feline leukemia virus were negative. fine-needle aspirates of mesenteric lymph nodes revealed the presence of banana-shaped apicomplexan parasites. the cat died after 4 days of hospitalization. postmortem polymerase chain reaction (pcr) analysis confirmed the presence of toxoplasma gondii in all examined organs. parasites were ex ...201121217037
use of crude, fml and rk39 antigens in elisa to detect anti-leishmania spp. antibodies in felis catus.visceral leishmaniasis is a disease caused by leishmania (leishmania) chagasi and represents a serious public health problem. the dog is the main urban reservoir of the disease; however, investigations regarding the occurrence and epidemiological importance of leishmaniasis in cats have recently been initiated. this study aimed to detect cats seropositive for leishmania spp. using different antigens. additional studies were performed using sera from cats with toxoplasma gondii (n=15) to evaluate ...201021195550
toxoplasma gondii antibodies on domiciled cats from lages municipality, santa catarina state, brazil.sera were collected from 300 domiciled cats from the municipality of lages, southern brazil, to determine the prevalence of toxoplasma gondii antibodies and risk factors associated. tests for t. gondii antibodies were performed using indirect immunofluorescent antibody test (ifat). positive reactions with titers ≥1:64 were found in 43 (14.33%) cats. a significant number of seropositive cats were ≥6 month old (p = 0.03758) and had access to the streets or/and rural areas (p = 0.04185). the result ...201021184709
prevalence and genotypes of toxoplasma gondii in feline faeces (oocysts) and meat from sheep, cattle and pigs in switzerland.the protozoan parasite toxoplasma gondii infects almost all warm blooded animal species including humans, and is one of the most prevalent zoonotic parasites worldwide. post-natal infection in humans is acquired through oral uptake of sporulated t. gondii oocysts or by ingestion of parasite tissue cysts upon consumption of raw or undercooked meat. this study was undertaken to determine the prevalence of oocyst-shedding by cats and to assess the level of infection with t. gondii in meat-producing ...201021183278
toxoplasma gondii antibodies in domestic cats in olsztyn urban area, poland.the aim of the investigation was to determine the prevalence of t. gondii among a domestic cat population in an urban area in olsztyn. in total, 135 serum samples of cats collected in several veterinary outpatient clinics in olsztyn were examined by direct agglutination assay. the toxo-screen da biomerieux commercial test detected anti-toxoplasma gondii igg antibodies. the results of studies indicated that cats bred under different conditions in the city of olsztyn have contact with various form ...201021174957
high prevalence of toxoplasmosis in cats from egypt: isolation of viable toxoplasma gondii, tissue distribution, and isolate designation.cats are important in the epidemiology of toxoplasma gondii because they are the only hosts that excrete environmentally resistant oocysts in feces. in the present study, 158 feral cats from giza, egypt, were examined for t. gondii infection. antibodies to t. gondii were found in 97.4% with the modified agglutination test. viable t. gondii was isolated from tissues (brain, heart, tongue) of 115 of 137 cats by bioassay in mice. these isolates were designated tgcateg 1-115; none of these isolates ...201021158619
genetic diversity of toxoplasma gondii isolates in egyptian feral cats reveals new genotypes.cats are important in the epidemiology of toxoplasma gondii because they are the only hosts that excrete environmentally resistant oocysts in feces. in the present study, 115 viable t. gondii isolates from tissues of cats from egypt were genotyped using 10 pcr-restriction fragment length polymorphism markers (sag1, sag2, sag3, btub, gra6, c22-8, c29-2, l358, pk1, and apico) and dna from tachyzoites. seven genotypes were recognized including the clonal type ii, type iii (2 genotypes), and 4 atypi ...201021158618
segmental meningomyelitis in 2 cats caused by toxoplasma gondii. 201021092003
infection dynamic of toxoplasma gondii in two fattening pig farms exposed to high and low cat density in an endemic region.the presence of cats in the farms is considered a risk factor for the infection of pigs with toxoplasma gondii (t. gondii). cats eliminate oocysts that contaminate food, water and promote the infection of host reservoir such as rodents and birds among others that are also involved in the infection of pigs. the objective of this study was to assess the dynamic of infection of t. gondii in seronegative weaned pigs from weaning to 20 weeks of age from two farms from an endemic region, one with high ...201021055875
multi-locus dna sequencing of toxoplasma gondii isolated from brazilian pigs identifies genetically divergent strains.five toxoplasma gondii isolates (tgpgbr1-5) were isolated from hearts and brains of pigs freshly purchased at the market of campos dos goytacazes, northern rio de janeiro state, brazil. four of the five isolates were highly pathogenic in mice. four genotypes were identified. multi-locus pcr-dna sequencing showed that each strain possessed a unique combination of archetypal and novel alleles not previously described in south america. the data suggest that different strains circulate in pigs desti ...201021051148
[a model of congenital toxoplasmosis transmission dynamics].toxoplasmosis is a parasitic zoonosis having worldwide distribution; it infects many human and animal populations and is produced by the parasite toxoplasma gondii, this being of great importance in contagion of pregnant women since this zoonosis causes illness in the fetus through transplacental infection. a constant mother-foetus infection rate has traditionally been used. nevertheless, there is evidence of a strong relationship between a mother's gestation week and the moment when a foetus be ...201021031242
serological and parasitological prevalence of toxoplasma gondii in wild birds from colorado.ground-feeding birds are considered important in the epidemiology of toxoplasma gondii because they serve as indicators of soil contamination by oocysts, and birds of prey are indicators of t. gondii prevalence in rodents and other small mammals. cats excrete environmentally resistant oocysts after consuming tissues of t. gondii -infected birds. in the present study, sera and tissues from 382 wild birds from colorado were tested for t. gondii infection. antibodies to t. gondii were found in 38 b ...201020950101
update on the diagnosis and management of toxoplasma gondii infection in cats. 201020937495
prevalence of toxoplasma gondii infection in stray and household cats in regions of seoul, korea.the principal objective of this study was to investigate the prevalence of toxoplasmosis in household and stray cats in seoul, republic of korea. we collected blood samples from 72 stray and 80 household cats, and all samples were examined by elisa and nested pcr. the overall positive rates of toxoplasma gondii in stray cats were 38.9% (28/72), with 15.3% (11/72) in elisa and 30.6% (22/72) in pcr. the positive rate in male stray cats was slightly higher than that of female stray cats. the highes ...201020877509
isolation and multilocus genotyping of toxoplasma gondii in seronegative rodents in brazil.synanthropic rodents, mainly rats and mice, become ecologically associated with men due to changes in their ecosystems caused by human activities. these animals may take part in the epidemiological cycles of several diseases, including toxoplasmosis. the presence of serum antibodies to toxoplasma gondii in 43 rodents captured in the urban area of umuarama, pr, brazil, was verified by modified agglutination test (mat). brain and heart samples were also collected and bioassayed in mice for the iso ...201020869811
low seroprevalence of leishmania infantum infection in cats from northern portugal based on dat and elisa.cats have been considered playing a role in the epidemiology of leishmaniasis caused by leishmania infantum, an endemic zoonosis in countries of the mediterranean basin. the present study assessed the prevalence of antibodies to l. infantum in 316 domestic cats from northern portugal, by means of the direct agglutination test (dat) and an enzyme-linked immunosorbent assay (elisa). seroreactivity to dat was found in six cats, and nine cats were positive in the elisa, including the six dat-positiv ...201020851524
bartonella species, feline herpesvirus-1, and toxoplasma gondii pcr assay results from blood and aqueous humor samples from 104 cats with naturally occurring endogenous uveitis.toxoplasma gondii, bartonella henselae and feline herpesvirus-1 (fhv-1) have been implicated as causative agents in feline uveitis. the usefulness of serum and aqueous humor (ah) antibody testing for these agents is limited as antibodies can be detected in both healthy cats and cats with uveitis. very few studies using polymerase chain reaction (pcr) assays to amplify organism dna from samples from cats with uveitis have been performed. in this study, assays to detect t gondii antibodies, feline ...201020817587
a serological and molecular study of leishmania infantum infection in cats from the island of ibiza (spain).the aim of this study was to evaluate the prevalence of leishmania infantum infection within a feline population by serologic and molecular methods and to identify associated risk factors. one hundred five cats living outdoors were studied. sera were tested for igg antibodies against l. infantum, toxoplasma gondii, and feline immunodeficiency virus (fiv) and for the detection of feline leukemia virus (felv) p27 antigen by enzyme-linked immunosorbent assay (elisa). l. infantum real-time polymeras ...201120804432
near-infrared raman spectroscopy to detect anti-toxoplasma gondii antibody in blood sera of domestic cats: quantitative analysis based on partial least-squares multivariate statistics.toxoplasmosis is an important zoonosis in public health because domestic cats are the main agents responsible for the transmission of this disease in brazil. we investigate a method for diagnosing toxoplasmosis based on raman spectroscopy. dispersive near-infrared raman spectra are used to quantify anti-toxoplasma gondii (igg) antibodies in blood sera from domestic cats. an 830-nm laser is used for sample excitation, and a dispersive spectrometer is used to detect the raman scattering. a serolog ...201020799833
seroprevalence of toxoplasma gondii infection amongst residents of tanga district in north-east tanzania.toxoplasmosis is a zoonotic disease, recognized as a serious public health problem worldwide. toxoplasma gondii infection has become a major public health concern in recent years due to the ravaging hiv/aids pandemic. a serological survey was carried out in tanga district of north-eastern tanzania to assess t. gondii infection rates among occupationally-exposed groups including abattoir workers, livestock keepers, animal health workers and other groups. the survey was undertaken in november 2005 ...200920734700
serological survey of toxoplasma gondii infection in the domestic goose (anser domestica) in southern china.in the present study, the antibodies to toxoplasma gondii in 191 farm-bred and 83 house-bred geese (anser domestica) were assessed for the prevalence of t. gondii infection in southern china with the modified agglutination test. antibodies to t. gondii (mat ≥ 1 : 5) were found in 27 (14.14%) of farm-bred geese and 14 (16.87%) of house-bred geese. geese infected with t. gondii may be a source of t. gondii infection for humans and cats.201120707861
transmission of toxoplasmosis (toxoplasma gondii) by foods.protozoan foodborne diseases are generally underrecognized. toxoplasma gondii is the causative agent of toxoplasmosis, one of the most prevalent parasitic infections to humans and domestic animals. the most likely source of t. gondii occurring through food is the consumption of raw or undercooked meat contaminated with tissue cysts. sporulated t. gondii oocysts, from the feces of infected cats, present in the environment are a potential source of infection. the ingestion of water contaminated wi ...201020691951
exposure to toxoplasma gondii in galapagos penguins (spheniscus mendiculus) and flightless cormorants (phalacrocorax harrisi) in the galapagos islands, ecuador.toxoplasma gondii is one of the most common protozoan parasites of humans and warm-blooded animals. members of the family felidae are the only definitive hosts of this parasite and, thus, important in the epidemiology of the disease. previous studies on pacific islands have found t. gondii infections in a number of avian species where domestic cats (felis catus) have been introduced. little is known about t. gondii in the galapagos islands, although introduced domestic cats in the archipelago ar ...201020688714
prevalence of antibodies to toxoplasma gondii in woodchucks across an urban-rural gradient.increasing urbanization has important consequences for wildlife, including the potential for higher prevalence of diseases within "urban adapter" species exposed to spillover from domestic animals. we investigated whether prevalence of antibodies to toxoplasma gondii in woodchucks (marmota monax) was related to urbanization in a midwestern landscape. we collected serum samples from adult woodchucks captured across an urban-rural gradient in illinois, usa in may-november 2007. we used an indirect ...201020688709
transmission dynamics of toxoplasma gondii along an urban-rural gradient.recently, several authors have proposed that the availability of intermediate hosts (ihs) for definitive hosts (dhs) may contribute to determining the dynamics and evolutionary ecology of parasites with facultative complex life cycles. the protozoa toxoplasma gondii may be transmitted to dhs either via predation of infected ihs through a complex life cycle (clc) or directly from a contaminated environment through a simple life cycle (slc). this parasite is also present in contrasting host densit ...201020685358
the occurrence of toxoplasma gondii infection in people and animals from rural environment of lublin region - estimate of potential role of water as a source of infection.a total of 254 humans and 489 domestic animals living on farms in the lublin province (eastern poland) were examined for the presence of antibodies against toxoplasma gondii using, respectively, the elfa and direct agglutination tests. in parallel, 182 samples of potable water, mostly from shallow household wells, were taken on farms and examined for the presence of t. gondii by microscopy and pcr. the frequency of seropositive reactions in farm inhabitants (66.9 percent) was significantly great ...201020684490
myelitis due to reactivated spinal toxoplasmosis in a cat.the diagnosis, management, and subsequent post-mortem confirmation of a case of suspected reactivated spinal toxoplasmosis in a 10-year-old female neutered cornish rex are described. while an ante-mortem diagnosis of toxoplasmosis was considered possible based on the neuroanatomical diagnosis of central nervous system (cns) disease primarily involving spinal cord segment c6-t2 and the progressive elimination of other potential causes, toxoplasma gondii antibody titres were consistent with previo ...201020674426
toxoplasma gondii strain-dependent effects on mouse behaviour.toxoplasma gondii reportedly manipulates rodent behaviour to increase transmission to its definitive feline host. we compared the effects of mouse infection by two type ii strains of t. gondii, prugniaud (pru) and me49, on attraction to cat odour, locomotor activity, anxiety, sensorimotor gating, and spatial working and recognition memory 2 months post-infection (mpi). attraction to cat odour was reassessed 7 mpi. at 2 mpi, mice infected with either strain exhibited significantly more attraction ...201020608478
the role of latent toxoplasmosis in the aetiopathogenesis of schizophrenia--the risk factor or an indication of a contact with cat?we assessed igg antibody to toxoplasma gondii in 300 inpatients with schizophrenia (sg), 150 outpatients with anxiety and depressive disorders (pcg), and 150 healthy blood donors (hcg). seropositivity rates were 60.7% for sg, 36.7% for pcg, and 45.3% for hcg (p<0.001). the seropositivity rate for anti-toxoplasma igg antibodies in sg was significantly higher that in pcg (chi2 = 23.11, or = 2.66, p = 0.001) and hcg (chi2 = 9.52, or = 1.86, p = 0.002). among sg, 85% of those who reported close cat ...201020608474
manipulation of host behaviour by toxoplasma gondii: what is the minimum a proposed proximate mechanism should explain?the behavioural manipulation hypothesis posits that parasites can change the behaviour of hosts to increase the reproductive fitness of the parasite. the protozoan parasite toxoplasma gondii fits this description well. sexual reproduction occurs in the cat intestine, from which highly stable oocysts are excreted in faeces. grazing animals, including rodents, can then ingest these oocysts. the parasite has evolved the capacity to abolish the innate fear that rodents have of the odours of cats, an ...201020608470
seroprevalence and risk factors associated with toxoplasma gondii in domestic pigs from spain.serum samples from 2970 (1400 sows, 1570 fattening) pigs, from 100 farms in the 10 main swine production regions in spain were tested for antibodies against t. gondii by the modified agglutination test (mat). antibodies to t. gondii (mat 1:25 or higher) were detected in 492 pigs (16.6%, 9.7% in fattening pigs and 24.2% in sows). the herd prevalence was 85.0% (95% ci: 78-92) and within-farm prevalence ranged from 2.9% to 92.8% (median=17.6%). statistically significant differences were observed am ...201020601110
epidemiology of toxoplasma gondii infection of stray cats in bangkok, thailand.the objective of this study was to investigate the epidemiology of toxoplasmosis in stray cats in bangkok. sera were collected during 2006 and examined by sabin-feldman dye test. five hundred sixty-four male and 926 female cats in and around monasteries from 50 districts were collected. toxoplasma gondii was detected in 72 (4.8%) of 1,490 cats. the prevalence was significantly higher in females (5.6%) than in males (3.6%). cats more than 5 years old had the highest infection rate (5.1%). fifty-s ...201020578476
toxoplasmosis in sand cats (felis margarita) and other animals in the breeding centre for endangered arabian wildlife in the united arab emirates and al wabra wildlife preservation, the state of qatar.the sand cat (felis margarita) is a small-sized felid found in sand and stone deserts ranging from the north of africa to asia, with the arabian peninsula as its centre of distribution. the sand cat captive breeding program at the breeding centre for endangered arabian wildlife (bceaw), sharjah, uae, has experienced high newborn mortality rates, and congenital toxoplasmosis was recently recognized as one of the causes of this mortality. in the present study, one 18-month-old sand cat (fm019) die ...201020570441
besnoitia neotomofelis n. sp. (protozoa: apicomplexa) from the southern plains woodrat ( neotoma micropus).certain species of the protozoan genus besnoitia cause clinical disease in livestock and wildlife. in the present paper a new species, besnoitia neotomofelis is described from the southern planes woodrat (neotoma micropus). the parasite was detected by bioassay of woodrat tissues in outbred swiss webster mice in an attempt to isolate toxoplasma gondii. initially, the organism was misdiagnosed as t. gondii because it was highly pathogenic for mice and its tachyzoites resembled t. gondii tachyzoit ...201020561393
seroepidemiology of toxoplasma gondii infection in patients with vision and hearing impairments, cancer, hiv, or undergoing hemodialysis in durango, mexico.toxoplasma gondii infection may cause a variety of symptoms involving virtually all organs. little is known of the epidemiology of t. gondii infection in different patient groups in mexico. we sought to determine the prevalence of t. gondii infection and associated epidemiological characteristics in 472 patients in durango, mexico. participants were tested for t. gondii igg and igm antibodies. in addition, sociodemographic, clinical, and behavioral characteristics from each participant were obta ...201020557194
decreased prevalence and age-specific risk factors for toxoplasma gondii igg antibodies in the netherlands between 1995/1996 and 2006/2007.to estimate the change in the seroprevalence and risk factors for toxoplasmosis in the netherlands, a study was conducted in the general population in 2006/2007, similarly designed as a previous study in 1995/1996. testing 5541 sera for igg antibodies against toxoplasma gondii showed a marked decrease of the overall seroprevalence to 26·0% [95% confidence interval (ci) 24·0-28·0], compared to 40·5% (95% ci 37·5-43·4) in 1995/1996. in women of reproductive age the seroprevalence decreased from 35 ...201120492743
a new atypical highly mouse virulent toxoplasma gondii genotype isolated from a wild black bear in alaska.most strains of toxoplasma gondii isolated in north america and europe are grouped into 3 (types i, ii, iii) genotypes and are considered clonal. recent evidence suggests that illness due to toxoplasmosis in immunocompetent persons may be related to infection with an atypical genotype; these strains are mouse virulent. in the present study, a new mouse-virulent atypical t. gondii genotype was isolated from an asymptomatic black bear ( ursus americanus ) from alaska. the bear had a titer of 1rati ...201020486739
new toxoplasma gondii genotypes isolated from free-range chickens from the fernando de noronha, brazil: unexpected findings.worldwide comparison of toxoplasma gondii isolates from free-range chickens ( gallus domesticus ) has indicated that t. gondii isolates from brazil are phenotypically and genetically different from isolates from other countries; most strains from brazil are pathogenic to mice, there is great genetic variability, most isolates are nonclonal, and type ii is absent or rare. the prevalence of t. gondii in 50 free-range chickens from the island of fernando de noronha, brazil (this island is 350 km fr ...201020486738
toxoplasmosis not transmitted by cat bite, but high prevalence of antibodies to toxoplasma gondii in patients bitten by their own cat.the aims of the study were to investigate the prevalence of antibodies to toxoplasma gondii in a group of patients bitten by cats, and also to determine if toxoplasmosis can be transferred by cat bite. seventy-two patients who attended the emergency wards at 3 hospitals in stockholm, sweden, due to infection by cat bite, were investigated for specific igm and igg antibodies to t. gondii in the acute phase, as well as in the convalescent phase about 2 weeks later. specific igg antibodies to t. go ...201020482458
seroepidemiology of toxoplasma gondii infection in a mennonite community in durango state, mexico.there is a lack of information concerning the epidemiology of toxoplasma gondii infection in mennonites (an ethnic group of mexican citizens of german descent living in rural communities). the prevalence of anti- t. gondii immunoglobulin (ig) g and igm antibodies was examined in 152 mennonites in durango state, mexico, using enzyme-linked immunoassays. in total, 46 (30.3%) of 152 participants (mean age 38.4 ± 15.5 yr) had igg t. gondii antibodies; 5 (3.3%) also had igm t. gondii antibodies. toxo ...201020481662
prevalence of selected infectious agents in cats in ireland.vector-borne bacterial and rickettsial agents and toxoplasma gondii, are common organisms in cats. some are potentially zoonotic or may be transmitted via blood transfusion. the current study investigated the prevalence of these agents in cats from dublin, ireland, for which no published data exists. whole blood (n=116) and sera (n=83) samples were obtained from 121 cats. dna was extracted from blood and assayed using polymerase chain reaction techniques for anaplasma species, bartonella species ...201020472484
serological survey of toxoplasma gondii in captive neotropical felids from southern brazil.toxoplasma gondii is the causative intracellular protozoan of toxoplasmosis in human being and animals. members of the felidae family are considered the single definitive host for the infection; both wild and domestic cats are able to excrete oocysts in the environment. wild cats maintained in captivity may serve as source of infection for other clinically susceptible animals in the same environment. the aim of this study was to determine the frequency of t. gondii igg antibodies in 57 neotropic ...201020472340
survey of infectious and parasitic diseases in stray cats at the lisbon metropolitan area, portugal.a survey of infectious and parasitic diseases of stray cats was carried out using biological samples collected from animals captured during a catch-neuter-release programme in four counties of the lisbon metropolitan area. the main objective was to investigate the potential threat of stray cats for animal and public health. samples of blood, stool, hair and auricular swabs were collected from 231 cats in 27 colonies. anti-toxoplasma gondii antibodies were detected in 47/194 samples (24.2%); anti ...201020466573
a comparative analysis of coprologic diagnostic methods for detection of toxoplama gondii in cats.the relative role of transmission of toxoplasma gondii infection from cats to humans appears to have recently increased in certain areas. large-scale screening of oocyst shedding in cats cannot rely on microscopy because oocyst identification lacks sensitivity and specificity, or on bioassays, which require test animals and weeks before examination. we compared a sensitive and species-specific coprologic-polymerase chain reaction (copro-pcr) for detection of t. gondii infected cats with microsco ...201020439968
[the effects of the infection caused by toxoplasma gondii on the cat duodenal wall].this paper analyzes the effects of the infection caused by toxoplasma gondii on the cat duodenal wall. six cats (felis catus) with 3-month-old were randomly divided into control group (g1; n = 3) and infected group (g2; n = 3). the animals from g2 received orarilly 200 t. gondii tissye cysts of me49-strain (type ii). after 40 days, the animals were submitted to euthanasia, laparotomy and had their duodenum removed, fixed in bouin solution and submitted to histological routine obtaining 3 μm tran ...201020385061
feline immunodeficiency virus, feline leukemia virus and toxoplasma gondii in stray and household cats in kerman-iran: seroprevalence and correlation with clinical and laboratory findings.this study was carried out to determine the seroprevalence of feline leukemia virus (felv), feline immunodeficiency virus (fiv) and toxoplasma gondii (t. gondii) infection among stray and owned cats in southeastern iran and to identify the influence of age, sex, lifestyle, health status, and laboratory findings on seropositivity. the overall infection rate for fiv, felv, and t. gondii was 19.2%, 14.2%, and 32.1% respectively. results of the multivariate logistic regression analysis showed that o ...201020362315
comparison of nested pcr and real-time pcr for the detection of toxoplasma gondii in biological samples from naturally infected cats.there are different protocols of molecular diagnosis methods available including dna extraction methods to diagnose of toxoplasma gondii, being necessary to perform comparative studies in biological samples. the aim of this study is to compare real-time pcr (rtpcr) and nested pcr (npcr) to evaluate the detection of t. gondii in naturally infected cats. biological samples of toxoplasma-seropositive cats were assayed for detection of t. gondii dna--extracted by both the lysis buffer and proteinase ...201020362309
seroprevalence of toxoplasma antibodies among individuals tested at hospitals and private laboratories in beirut.toxoplasmosis is a zoonotic disease of worldwide distribution. infection with toxoplasma gondii can result in debilitating disease especially in the immunocompromized host and pregnant women. determination of its seroprevalence and risk factors have been studied in many countries as a mean to understand its epidemiology and implement control measures. the scarcity of information on t. gondii infection in lebanon warranted studying the toxoplasma antibody findings among individuals tested at diff ...201020358853
seroprevalence of toxoplasma gondii in nazareth town, ethiopia.to determine seroprevalence of toxoplasmosis, assess its zoonotic importance and identify factors associated with seroprevalence.200720353125
modeling toxoplasmosis spread in cat populations under vaccination.in this paper we present an epidemiological model to study the transmission dynamics of toxoplasmosis in a cat population under a continuous vaccination schedule. we explore the dynamics of toxoplasmosis at the population level using a mathematical model that includes the effect of oocyst, since the probability of acquisition of toxoplasma gondii infection depends on the environmental load of the parasite. this model considers indirectly the infection of prey through the oocyst shedding by cats. ...201020304000
conventional and molecular diagnostic testing for the acute neurologic patient.the aim of this review is to describe and evaluate both conventional and molecular diagnostic testing utilized in dogs and cats with acute neurologic diseases. various types of polymerase chain reaction (pcr) are explored along with novel molecular diagnostic testing that ultimately may prove useful in the critical care setting.201020230434
seroprevalence and associated risk factors of toxoplasmosis in pregnant women in hebron district, palestine.to measure the prevalence of toxoplasmosis, we tested 204 pregnant women for igg and igm antibodies to toxoplasma gondii using an enzyme-linked immunoassay. the study was conducted in hebron district during the year 2005. the seroprevalence of igg antibodies to t. gondii was 27.9% from rural areas (36.8%) had igg antibodies to t. gondii than urban women (21.4%). possible routes of infection were contaminated soil, drinking rainwater and eating raw vegetables rather than eating uncooked meat or c ...200920214142
toxoplasma gondii: epidemiology, feline clinical aspects, and prevention.toxoplasma gondii is a parasite of birds and mammals. cats are the only definitive host and thus the only source of infective oocysts, but other mammals and birds can develop tissue cysts. although feline infections are typically asymptomatic, infection during human pregnancy can cause severe disease in the fetus. cat owners can reduce their pets' exposure risk by keeping all cats indoors and not feeding them raw meat. humans usually become infected through ingestion of oocyst-contaminated soil ...201020202907
seroprevalence and risk factors associated with toxoplasma gondii infection in pig farms from catalonia, north-eastern spain.seroprevalence and associated risk factors for toxoplasma gondii infection in pigs were analyzed in 1202 sera samples, including sows and pigs of three, seven, 11, 15 and 20 weeks of age, from 23 farms in catalonia, north-eastern spain. antibodies were tested by the modified agglutination test (mat) at titers > or =1:25. antibodies to t. gondii were found in 228 samples (19.0%; 95% ci: 16.8-21.2). the individual prevalence in animals higher than 7 weeks of age was 22.8% (174/762; 95% ci: 16.6-29 ...201020189210
the role of dna microarrays in toxoplasma gondii research, the causative agent of ocular toxoplasmosis.ocular toxoplasmosis, which is caused by the protozoan parasite toxoplasma gondii, is the leading cause of retinochoroiditis. toxoplasma is an obligate intracellular pathogen that replicates within a parasitophorous vacuole. infections are initiated by digestion of parasites deposited in cat feces or in undercooked meat. parasites then disseminate to target tissues that include the retina where they then develop into long-lived asymptomatic tissue cysts. occasionally, cysts reactivate and growth ...200920157353
a prospective study of toxoplasma-positive pregnant women in southern brazil: a health alert.we evaluated anti-toxoplasma gondii igm-reactive pregnant women seen at a high-risk pregnancy outpatient clinic. from march 2005 to january 2008 in paraná, brazil, pregnant women seen by the brazilian public health system, in any gestational period, who were anti-t. gondii igm-positive, were followed. clinical symptoms were noted, and tests performed including iga, igg avidity, ultrasonogram, and amniocentesis (pcr/inoculation in mice). of 75 patients, 8 showed low, 3 intermediate and 31 high ig ...201020138322
seroprevalence and factors associated with toxoplasma gondii infection in wild boar ( sus scrofa) in a mediterranean island.knowledge of the factors affecting the presence of toxoplasma gondii in wildlife is limited. here we analyse which local landscape characteristics are associated with the presence of toxoplasmosis in wild boar, sus scrofa, on the island of corsica, france. meat juice samples from 1399 wild boars collected during two hunting seasons were tested for t. gondii antibodies using the modified agglutination test (titre 1:4). the overall seroprevalence was 0.55 (95% ci 0.50-0.59) for the first year and ...201020096148
[serological study on toxoplasmosis among pregnant women from franceville, gabon].the serological prevalence of toxoplasma gondii was studied among 839 pregnant women in two hospitals from franceville (gabon), between may 2007 and december 2007. specific t gondii igg and igm were measured by enzyme linked fluorescent assay (elfa). datation of the infection was carried out by avidity test. fifty-six percent of women in this study were immunised compare to the 71% who were found as immunised in a previous study carried out fifteen years ago. 2.6% were found to be igm positive. ...201020084487
use of protein ag in an enzyme-linked immunosorbent assay for serodiagnosis of toxoplasma gondii infection in four species of animals.an enzyme-linked immunosorbent assay (elisa) employing protein ag (ag-elisa) as a conjugate was developed to detect anti-toxoplasma gondii antibodies (ab) in experimentally infected pigs and naturally infected pigs, goats, dogs, and cats. the results indicate that ag-elisa can be a useful method for serological diagnosis of t. gondii infection in these four species of animals.201020071493
[zoonotic parasites of cats and dogs found in playground sandboxes in the reykjavik area, iceland.]recent surveys on the parasites of household cats and dogs in iceland have revealed the zoonotic protozoans cryptosporidium parvwn and toxoplasma gondii and the zoonotic nematodes toxocara cati and t. canis. furthermore, a giardia sp., recently found in cats in iceland, is also suspected to be a zoonotic parasite. in iceland children frequently play in open sandboxes commonly found at kindergartens, in public areas or in private gardens. during the cold months of the year, when the soil is frequ ...199620065440
long-term survival of toxoplasma gondii sporulated oocysts in seawater.toxoplasma gondii is now recognized as an important pathogen in costal marine mammals. oocysts from cat feces are believed to be washed into seawater and serve as a source of infection via transport hosts. experimentally, it has been demonstrated that t. gondii oocysts can sporulate in seawater and remain infectious for mice for up to 6 mo. the present study examined the long-term survival of t. gondii in seawater (15 ppt nacl) kept at 4 c or at room temperature. oocysts kept at 4 c for 24 mo we ...200920050010
ovine toxoplasmosis.congenital infection with toxoplasma gondii is an important cause of abortion in sheep worldwide. the cat is the definitive host of the parasite, and infected cats may shed millions of oocysts in their faeces resulting in extensive environmental contamination and an important source of infection for grazing herbivorous animals. studies looking at development of specific antibodies in sheep, as an indicator of exposure to t. gondii, have shown that there is an increase in seroprevalence associate ...200919995468
epidemiological study of toxoplasma gondii infection in ovine breeding.an outbreak of toxoplasmosis occurring in a typical farm of 524 ovines was monitored for 1 year after the occurrence of 31 abortions. abortion events involved 7.2% of 430 pregnant sheep. presence of antibodies to toxoplasma gondii in sheep sera was investigated by the indirect fluorescence antibody test (ifat). a total of 422 ewes were bled four times during the year, and an epidemiological analysis was performed on all serology data collected in this subgroup. the prevalence of igg positives ra ...201019968848
genotype of toxoplasma gondii from blood of stray cats in gyeonggi-do, korea.genotyping of toxoplasma gondii has been performed in 23 pcr positive blood samples from stray cats in korea. we used 2 separate pcr-restriction fragment length polymorphism (rflp) patterns of sag2 gene, amplifying the 5' and 3' ends of the locus. the results revealed that all samples belonged to the type i clonal lineage. although t. gondii organisms were not isolated from the samples, the results of the present study represent that stray cats with t. gondii infection should be seriously concer ...200919967093
local meteorological conditions, dynamics of seroconversion to toxoplasma gondii in cats (felis catus) and oocyst burden in a rural environment.the aim of this study was to analyse the spatio-temporal dynamics of toxoplasma gondii infection in long-term monitoring of domestic cats (8-15 years) in three populations living in rural france. overall seroprevalence was 52.7% (modified agglutination test > or =1:40). incidence was 0.26-0.39 seroconversions/cat per year, and the estimated rate of soil contamination by t. gondii oocysts ranged between 31 and 3600 oocysts/m2 per year, depending on the population. incidence risk in cats was relat ...201019961642
toxoplasmosis and other intestinal coccidial infections in cats and dogs.toxoplasma gondii and related coccidians are intracellular protozoan parasites. coccidia are obligate intracellular parasites normally found in the intestinal tract. virtually all warm blooded animals, including humans are commonly infected with coccidians. this article reviews the diagnosis, treatment, and prevention of infections in cats and dogs related to isospora spp, toxoplasma gondii, and neospora caninum. much remains to be learned concerning the pathogenesis of clinical coccidiosis.200919932360
obtaining highly purified toxoplasma gondii oocysts by a discontinuous cesium chloride gradient.toxoplasma gondii is an obligate intracellular protozoan pathogen that commonly infects humans. it is a well characterized apicomplexan associated with causing food- and water-borne disease outbreaks. the definitive host is the feline species where sexual replication occurs resulting in the development of the highly infectious and environmentally resistant oocyst. infection occurs via ingestion of tissue cysts from contaminated meat or oocysts from soil or water. infection is typically asymptoma ...200919888193
factors affecting seroprevalence of toxoplasma gondii in the endangered iberian lynx (lynx pardinus).wild felids are considered important in maintaining the sylvatic cycle of toxoplasma gondii. although, t. gondii antibodies have been reported in several species of wild felids, little is known of the epidemiology and risk factors associated with t. gondii infection in wild cats. the iberian lynx (lynx pardinus) is the most endangered felid species in the world. in the present study, seroprevalence and associated risk factors for t. gondii infection in a large population of iberian lynx in spain ...201019879052
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