| prevalence of feline immunodeficiency virus and feline leukemia virus infections in random-source cats. | retroviral serologic profiles were generated for 506 random-source cats (felis catus) that were received by our facility during a twenty-month period. feline leukemia virus antigens were detected in plasma samples from 26 (5.1%) of the cats. antibodies to feline immunodeficiency virus were present in 24 (4.7%) of the samples tested. a single cat (0.2%) was positive for both viruses. neither gender nor vendor correlation with retroviral seropositivity could be demonstrated. | 1991 | 1667194 |
| molecular analysis of several classes of endogenous feline leukemia virus elements. | five recombinant dna clones of endogenous feline leukemia virus-related dna sequences were isolated by screening a lambda phage genomic library of cat placental dna with a probe specific to the gag-pol region of infectious feline leukemia virus. the clones containing retroviral long terminal repeat-like sequences demonstrated the existence of different size classes of endogenous elements in the cat genome, including those of nearly full length in which the gag region is heterogeneous but all of ... | 1985 | 2415715 |
| flvi-1, a common integration domain of feline leukemia virus in naturally occurring lymphomas of a particular type. | a locus in feline dna, termed flvi-1, which may play an important role in the natural induction of lymphomas by feline leukemia virus (felv) was identified. examination of a bank of 21 naturally occurring felv-positive feline lymphomas revealed that felv proviral integration occurs at flvi-1 in four independent tumors (19%). independent integrations occurred within a 2.4-kilobase region of flvi-1, the probability of which by random chance can be estimated as 10(-16). several lines of evidence, i ... | 1990 | 2161948 |
| molecular cloning of feline leukemia provirus genomes integrated in the feline large granular lymphoma cells. | feline leukemia virus (felv) is a horizontally transmitted agent of the domestic cat which is known to be associated with wide spectrum of diseases of the hematopoietic system. in the present study, proviral dnas of felv proviruses were examined in the tumor cells of natural killer cell lineage which is very rare in cats. in the chromosomal dna of the tumor cells, 5 distinct bands corresponding to exogenous felv provirus genomes were detected by digestion with ecori which does not cut most felv ... | 1990 | 2162159 |
| should cats be vaccinated for feline leukemia? | | 1990 | 2163809 |
| characterization and significance of delayed processing of the feline leukemia virus felv-faids envelope glycoprotein. | felv-faids, an immunodeficiency-inducing isolate of feline leukemia virus, is composed of a pathogenic but replication-defective genome (molecular clone 61c) and a replication-competent but non-immunodeficiency-inducing variant genome (molecular clone 61e). the chimeric virus eecc, composed of the 5' gag-pol of 61e fused to the env-3' ltr of 61c, also induces immunodeficiency. the 61c (or eecc) gp80 can be distinguished from that of 61e on the basis of antigenic recognition, size, and rate of po ... | 1990 | 2166820 |
| [feline immunodeficiency virus in switzerland: clinical aspects and epidemiology in comparison with feline leukemia virus and coronaviruses]. | serum samples from 1421 domestic cats (561 healthy, 860 sick) were tested for fiv-, felv- and coronavirus infection. the results were stored in a computer data base and compared with epidemiologic data and clinical findings. all 3 infections were significantly more prevalent in sick than healthy cats: fiv was found in 0.7% of healthy and 3.4% of sick cats. for felv the prevalence was 3.0% and 13.0% and for coronavirus 21% and 36.2%, respectively. fiv-infected cats were mostly male (73%); no sex ... | 1990 | 2167513 |
| vaccination against feline retroviruses. | | 1990 | 2178114 |
| feline lymphomas: immunological and cytochemical characterization. | the immunological and cytochemical phenotypes of five primary feline lymphomas and six feline lymphoma lines are reported. thymic lymphomas induced by the rickard strain of felv (felv-r) are of prothymocyte or (immature) cortical thymocyte origin, as these express terminal deoxynucleotidyl transferase, the guinea pig erythrocyte rosette receptor, ia antigens, partial cortisone sensitivity, and nonspecific esterase. lymphomas associated with other strains of felv form rosettes with guinea pig ery ... | 1989 | 2535958 |
| feline leukemia virus and feline immunodeficiency virus infections in a cat with lymphoma. | lymphoma was diagnosed in a 7-year-old domestic cat found to be infected with felv and feline immunodeficiency virus (fiv). the cat was affected by chronic disorders suggestive of immunosuppression, including gingivitis, periodontitis, keratitis, and abscesses. despite treatment, peripheral keratitis of the left eye progressed, resulting in uveitis, chronic glaucoma, and eventual corneal rupture. microscopic retinal and optic disk pathologic processes also were suspected. abnormal jaw movements ... | 1989 | 2537274 |
| evaluation of humoral immunity tests in felv infected cats. | | 1990 | 2178116 |
| retroviruses and malignant lymphoma. | retroviruses are single stranded rna viruses that possess a unique enzyme, reverse transcriptase. this enzyme facilitates the synthesis of double stranded dna intermediates able to integrate into the genome of the host cell. although primarily a means of remaining latent, this integration can have the effect of transforming the cell to malignant growth, either by activation of growth control genes in cis or trans, or by insertion of an integrated oncogene. malignant lymphomas caused by retroviru ... | 1989 | 2541838 |
| feline immunodeficiency virus and feline leukemia virus infections and their relationships to lymphoid malignancies in cats: a retrospective study (1968-1988). | sera from 353 cats with naturally occurring feline leukemia virus (felv) infection were collected in boston, los angeles, new york city, and seattle between 1968 and 1988. these sera were retrospectively assayed by enzyme-linked immunosorbent assay for antibodies to feline immunodeficiency virus (fiv). fifty-one (14.4%) of the felv-positive sera had antibodies to fiv, indicating dual oncovirus and lentivirus infections. fiv infections were confirmed by western blot analysis, antibodies against t ... | 1990 | 2159993 |
| a feline large granular lymphoma and its derived cell line. | a lymphoma cell line (mcc) was derived from an abdominal mass from a 13-yr-old castrated male cat. the cells resemble natural killer precursor cells, have membrane-bound granules, and are positive for chloroacetate esterase, alpha-naphthyl butyrate esterase, and tartrate-resistant acid phosphatase activities. the mcc cells are negative for rearranged feline t-cell receptor genes, negative for feline t-cytotoxic antigen, ia, and surface mu, tau, and lambda chains and do not form e-rosettes. the m ... | 1990 | 2161827 |
| existence of feline immunodeficiency virus infection in japanese cat population since 1968. | | 1990 | 2168001 |
| recombinant feline herpesviruses expressing feline leukemia virus envelope and gag proteins. | we constructed recombinant feline herpesviruses (fhvs) expressing the envelope (env) and gag genes of feline leukemia virus (felv). expression cassettes, utilizing the human cytomegalovirus immediate-early promoter, were inserted within the thymidine kinase gene of fhv. the felv env glycoprotein expressed by recombinant fhv was processed and transported to the cell surface much as in felv infection, with the exception that proteolytic processing to yield the mature gp70 and p15e proteins was les ... | 1990 | 2168977 |
| hematologic manifestations of feline immunodeficiency virus infection. | studies were done on 53 cats with community-acquired infection with the feline immunodeficiency virus (fiv) to determine if hematologic abnormalities were comparable with those observed in patients seropositive for the human immunodeficiency virus (hiv). nine cats were asymptomatic, 24 had clinical symptoms equivalent to aids-related complex (arc), and 20 had aids-like disease. hematologic abnormalities were detected in 75% (40 of 53) of fiv-seropositive cats, and multiple concurrent cytopenias ... | 1990 | 2400806 |
| feline leukemia virus-induced immunodeficiency syndrome in cats as a model for evaluation of antiretroviral therapy. | severe progressive immunodeficiency syndrome can be induced experimentally with a molecularly cloned isolate of feline leukemia virus (felv-faids). the resultant disease syndrome is characterized by persistent viremia, lymphopenia, progressive weight loss, persistent diarrhea, enteropathy, and opportunistic infections. the onset of clinical immunodeficiency disease is prefigured by the replication of the felv-faids variant virus in bone marrow and other tissues. the felv-faids system can be used ... | 1989 | 2540109 |
| on interpretation of feline leukemia test results. | | 1989 | 2557312 |
| characterization of a newly established feline lymphoma-derived cell line (bkd) lacking t and b cell surface markers. | a new feline lymphoma-derived cell line, designated bkd, was isolated from an anterior mediastinal tumor. cells of this line were characterized as lymphoid based on morphology, the lack of intracellular esterase and peroxidase activity, and absence of phagocytic function. in contrast with other established feline lymphoma-derived cell lines, cells of the bkd line lack characteristics of both feline t-cells and b-cells in that they neither form rosettes with guinea pig erythrocytes nor have demon ... | 1986 | 2423498 |
| effects of cobra venom factor treatment on latent feline leukemia virus infection. | the role of the complement system in containment of feline leukemia virus infection was studied by cobra venom factor treatment of feline leukemia virus-immune cats. one to three weeks after cobra venom factor treatment, an increase in viral antigen in marrow myelomonocytic cells and circulating immune complexes was noted. prevention of reactivation of feline leukemia virus infection may in part depend on an intact complement system. | 1985 | 2987539 |
| structural abnormality and over-expression of the myc gene in feline leukemias. | tumor cells of feline leukemia were examined for structural abnormality and expression of the myc gene. four of 12 spontaneous leukemia cases showed structural abnormalities of the myc gene detected by southern blotting analysis. of these 4 cases, one (case 1) showed a dna rearrangement in the 5' portion of the c-myc locus associated with integration of feline leukemia virus (felv) genome. another case had a newly inserted myc gene which was transduced by felv. in the 2 other cases, no relation ... | 1987 | 2822586 |
| effects of treatment with cobra venom factor on experimentally induced feline leukemia. | five- to six-month-old specific-pathogen-free cats were exposed to cobra venom factor (cvf) alone (4 cats), rickard feline leukemia virus (felv; 9 cats), or cvf and felv (6 cats). host-virus relationships were evaluated by monitoring the development of viremia, production of antibody against feline oncornavirus-associated cell membrane antigen, and amount of circulating immune complexes (cic). exposure to cvf induced complement depletion, which lasted 8 to 15 days. however, complement depletion ... | 1987 | 2820273 |
| feline leukemia virus: current status of the feline induced immune depression and immunoprevention. | this is a review of the current knowledge of feline leukemia virus (felv) associated with immune depression observed in cats. it will focus on the clinical and experimental observations associated with feline retroviral infection and presence in vivo and in vitro. we will briefly describe retroviral-associated acquired immune deficiency syndrome associated with felv infection in the cat and specific cellular pathology associated with felv latency. in addition, we will focus on the action of felv ... | 1987 | 2826030 |
| [cat retroviruses and human retroviruses: elements of comparison]. | following emotional head-lines of certain articles in the press, making believe that the cat could be susceptible to the aids virus, the authors present elements of comparison between principal feline retroviruses (the feline leucosis virus and the feline immunodeficiency virus) and the two human immunodeficiency viruses (hiv). the feline leucosis virus in differentiated from the human and the feline immunodeficiency viruses by its virological, pathological and epidemiological characteristics. b ... | 1989 | 2553219 |
| murine monoclonal antibodies cross-reactive with feline peripheral blood mononuclear cells. | twelve murine monoclonal antibodies (mab), specific for canine, human, and mouse cell surface determinants on lymphohemopoietic cells, were tested for reactivity (using indirect immunofluorescence) with peripheral blood mononuclear cells (pbmc) from 3 normal cats and 3 felv-positive cats with lymphoblastic leukemia. mab dly6 and 1h3, specific for canine lymphocytes, mab hb57s, specific for human mu(igm) chain, mab j-118 40.164.3 and h81.98.71, specific for murine and human ia, all had higher bin ... | 1987 | 2829413 |
| animal models of aids. | animal models of aids are essential for understanding the pathogenesis of retrovirus-induced immune deficiency and encephalopathy and for development and testing of new therapies and vaccines. aids and related disorders are etiologically linked to members of the lentivirus subfamily of retroviruses; these lymphocytopathic lentiviruses are designated human immuno-deficiency virus type 1 (hiv-1) and human immuno-deficiency virus type 2 (hiv-2). the only animals susceptible to experimental hiv-1 in ... | 1989 | 2556312 |
| use of a subunit feline leukemia virus vaccine in exotic cats. | three adult bengal tigers, 2 immature white tigers, and 3 adult servals were vaccinated im with three 1-ml doses of a subunit felv vaccine with dosage interval guidelines of the manufacturer. all cats had increased antibody titers to felv gp 70 capsular antigen and feline oncornavirus cell membrane-associated antigen during the vaccination trial. three weeks after the third vaccination, 7 of the 8 cats had gp70 antibody titers greater than 0.2 (optical density), and all 8 cats had feline oncorna ... | 1988 | 2835347 |
| effect of diethylcarbamazine on serum antibody to feline oncornavirus-associated cell membrane antigen in feline leukemia virus cats. | diethylcarbamazine (n,n-diethyl-4-methyl-1-piperazine carboxamide; dec) is a drug frequently used for prevention and treatment of the filariases. an opsonic action of dec may generate increased immune responses to microfilariae. we tested the hypothesis that dec treatment could result in higher antibody levels to other infectious agents. a retroviral animal model was studied, in light of the consideration that use of dec as an antifilarial agent could conceivably alter seroepidemiologic surveys ... | 1988 | 2836593 |
| structure and function of endogenous feline leukemia virus long terminal repeats and adjoining regions. | the nucleotide sequence of the 5' long terminal repeat (ltr) of three independent loci (cfe-6, cfe-16, and cf-14) of endogenous feline leukemia virus (felv) dnas of the domestic cat genome was determined. the 3' ltr of the cfe-6 clone was also sequenced. the endogenous felv ltrs, which were very similar to each other in sequence and in organization of the functional domains, differed considerably from the exogenous felv ltr in the u3 region. the major differences in u3 included variations in set ... | 1988 | 2843665 |
| fluorometric enzyme immunoassay for measurement of infectious feline leukemia virus and its neutralization. | a fluorometric enzyme immunoassay using an alkaline phosphatase-conjugated monoclonal antibody was developed to quantitate feline leukemia virus (felv) infection. monoclonal antibodies, directed against the felv structural protein p27, were conjugated with alkaline phosphatase using a modified maleimide method. the enzyme immunoassay requires only 4 days to reproducibly measure felv production instead of the 12 days required for the commonly used transformation assay using c81 cells. a linear co ... | 1988 | 2846700 |
| transmission of feline leukaemia virus in the milk of a non-viraemic cat. | the possibility of the transmission of feline leukaemia virus (felv) from latently infected cats was studied. five female cats with latent infections were examined for evidence of transmission of the virus to their kittens. one of the cats infected members of four consecutive litters of kittens which subsequently became persistently viraemic and transmitted the virus to other susceptible kittens by contact. shortly after birth its kittens were apparently felv-free since neither viral antigen nor ... | 1986 | 3012849 |
| tumorigenic potential of a myc-containing strain of feline leukemia virus in vivo in domestic cats. | the oncogenic capacity of a myc-containing strain of feline leukemia virus (felv), termed lc-felv, has been examined after inoculation of the virus into neonatal kittens. like other myc-containing strains of felv, lc-felv may induce with relatively short latency, but does not necessarily induce, thymic lymphosarcoma in viremic animals. naturally occurring and experimentally induced tumors are t-cell lymphomas which contain clonally integrated lc-felv proviral dna and which cannot readily be cult ... | 1988 | 2846887 |
| feline immunodeficiency syndrome--a comparison between feline t-lymphotropic lentivirus and feline leukemia virus. | a feline t-lymphotrophic lentvirus (ftlv) has recently been isolated from a domestic cat free of feline leukemia virus (felv). this virus is distinct from felv (an oncornavirus), although they share a common denominator, namely, the ability to cause immunosuppression and induce lymphadenopathy and anemia. their differences can be revealed by examining the following: the metal requirement for reverse transcriptase activity, the antigenic comparison by western blot analysis, the different suscepti ... | 1988 | 2848998 |
| treatment of felv-induced immunodeficiency syndrome (felv-faids) with controlled release capsular implantation of 2',3'-dideoxycytidine. | 2',3'-dideoxycytidine (ddc) inhibits replication of the immunodeficiency inducing strain of feline leukemia virus (felv-faids) in vitro at concentrations ranging from 1-10 micrograms/ml. additive antiviral effect is achieved when ddc is combined with either human recombinant alpha interferon (ifn alpha) or tumor necrosis factor (tnf) plus ifn alpha. initial in vivo pharmacokinetic studies in cats, utilizing bolus intravenous administration of ddc (20 mg/kg), resulted in peak plasma concentration ... | 1989 | 2544137 |
| humoral immune response of asymptomatic cats naturally infected with feline leukemia virus. | the humoral immune response of cats that were naturally infected with the feline leukemia virus (felv) was examined after antigenic stimulation with the synthetic antigen poly(l-tyr, l-glu)-poly(dl-ala)-poly(l-lys). the primary humoral antibody response in felv-infected cats was both delayed and greatly reduced, compared with that seen in uninfected control cats. a similar discordance was observed after secondary stimulation with the antigen, in the felv-infected cats had both a delayed response ... | 1986 | 3022000 |
| epizootiologic survey of feline leukemia virus in south-kyushu area. | | 1985 | 2995717 |
| transduction of endogenous envelope genes by feline leukaemia virus in vitro. | feline leukaemia viruses (felv) are exogenous retroviruses that can be detected in most cats with leukaemia, aplastic anaemia, myeloproliferative diseases and fatal immunosuppression. felv isolates have been divided into three subgroups, based on the viral envelope-determined properties of interference and host range in vitro. felv-a is present in all natural isolates and is generally minimally pathogenic. felv-b is found with felv-a in isolates from approximately 40% of natural infections and i ... | 1988 | 2895894 |
| experimental transmission and pathogenesis of immunodeficiency syndrome in cats. | we describe the identification, experimental transmission, and pathogenesis of a naturally occurring powerfully immunosuppressive isolate of feline leukemia virus (designated here as felv-faids) which induces fatal acquired immunodeficiency syndrome (aids) in 100% (25 of 25) of persistently viremic experimentally infected specific pathogen-free (spf) cats after predictable survival periods ranging from less than 3 months (acute immunodeficiency syndrome) to greater than one year (chronic immunod ... | 1987 | 2823940 |
| feline haemobartonellosis: clinical, haematological and pathological studies in natural infections and the relationship to infection with feline leukaemia virus. | haemobartonella felis infection was demonstrated in 38 cats which could be divided into four groups as follows: group a, feline leukaemia virus (felv) free cats with h felis infection alone; group b, felv free cats with h felis infection and other clinical conditions; group c, felv positive cats with h felis infection but no clinical manifestation of felv related or any other intercurrent disease; and group d, felv positive cats with h felis infection and clinical manifestations of felv related ... | 1988 | 2834861 |
| igg-fc-receptor positive and negative t cells from feline leukemia virus-infected cats. | | 1988 | 2850387 |
| feline cytotoxic large granular lymphocytes induced by recombinant human il-2. | large granular lymphocytes (lgl) have been characterized phenotypically and functionally as cytotoxic t lymphocytes, nk cells or lymphokine-activated killer cells. the most prominent morphologic feature of lgl is large cytoplasmic granules that are thought to contain the molecules responsible for cell lysis. in this study, we describe the morphologic and functional characteristics of il-2-dependent cytotoxic lymphocytes derived from feline pbl. stimulation of feline pbl with con a followed by cu ... | 1989 | 2544649 |
| induction of aplastic anemia by intra-bone marrow inoculation of a molecularly cloned feline retrovirus. | intra-bone marrow inoculation of cells infected with molecularly cloned feline retrovirus (felv-c-sarma [fsc]) associated with aplastic anemia was examined to test the hypothesis that cell-to-cell transmission of virus might facilitate marrow cell infection and anemogenesis, a possibility suggested by in-vitro co-culture experiments. ibm inoculation of either fsc-infected feline marrow cells or fibroblasts of weanling cats bypassed age-related restriction of fsc replication, initiated viremia, c ... | 1989 | 2552228 |
| diagnosis of feline leukemia virus and feline immunodeficiency virus infections. | feline leukemia virus is an oncogenic retrovirus that can result in a wide variety of neoplastic and non-neoplastic diseases, including immunosuppression. diagnosis of felv infection can be achieved by several methods, including virus isolation; ifa assay of a peripheral blood smear; and detection of a viral protein (called p27) by elisa testing of whole blood, plasma, serum, saliva, or tears. commercially available elisa kits have revolutionized felv testing and have become very popular as "in- ... | 1989 | 2549672 |
| isolation from cats of an endogenous type c virus with a novel envelope glycoprotein. | a search for variant endogenous cat viruses led to a novel isolate. although the major envelope glycoprotein of this virus was similar in size to that of an rd-114-like virus that was coisolated, it was unrelated to rd-114 or feline leukemia virus by immunological and biological criteria. this degree of dissimilarity suggests a different evolutionary progenitor from that for the rd-114 and feline leukemia virus viral envelopes. the novel virus did, however, code for gag gene polypeptides which a ... | 1985 | 2983093 |
| immunodeficiency in latent feline leukemia virus infections. | challenge of naive experimental animals with a retroviral inoculum may result in one of two broad sequelae. the first is the establishment of an appropriate humoral and cellular immune response leading to a condition of immunity to subsequent infection with the retrovirus. alternatively, the host may fail to develop a successful immune response, resulting in a chronic viremia associated with immunosuppression and ultimately death due to secondary pathogens. an alternate disease course is the est ... | 1989 | 2549692 |
| ultrastructural morphology of leukemic cells in the cat. | transmission and scanning electron microscopic characteristics of leukemic cells from eight cats with spontaneous leukemia are described. nuclear blebs, myelin figures, cytoplasmic microfibrils and c-type virus were seen more frequently in leukemic cells. surface ridges and ruffles seen with the scanning electron microscopy were helpful in distinguishing myelogenous from lymphogenous leukemia. | 1985 | 2984829 |
| control of feline leukaemia virus. | feline leukaemia virus (felv) usually occurs in its natural species, the domestic cat. felv is also important to human individuals as a comparative model, as it may cause a variety of diseases, some malignant and some benign, such as immunosuppression, which bears a resemblance to aids (acquired immune deficiency syndrome) in man. felv is transmitted among cats by contagion. the main sources of infection are persistently infected carrier cats which continuously excrete virus. dissemination of fe ... | 1989 | 2549695 |
| selective removal of antigen-complexed igg from cat plasma by adsorption onto a protein a-silica matrix. | the binding of normal cat igg, heat-aggregated cat igg and specific immune complexes (ic) containing cat igg to a silica matrix containing covalently bound staphylococcus aureus protein a was evaluated. the amounts of serum relative to protein a-silica, the flow rates and the perfusion times were representative of those existing when protein a-silica columns are used for therapeutic extracorporeal immunoadsorption of igg and ic from humans and animals. when cat igg was present in a large excess, ... | 1987 | 3039006 |
| killer cells of feline leukemia virus- and feline sarcoma virus-infected transformed cells: the role of nk, adcc, and in vitro generated cytotoxic cells. | feline white blood cells (wbc) manifested a primary in vitro mixed lymphocyte tumor cell culture (mltc) proliferative response to feline leukemia virus-feline sarcoma virus (felv-fesv)-infected transformed target cells, which reached a peak at day 15. furthermore, primary in vitro mltc cultures generated cytotoxic killer cells capable of killing a variety of targets in non-major histocompatibility gene complex restricted fashion, and effector cells were capable of killing targets introduced repe ... | 1985 | 2992811 |
| quantitation of specific antibodies bound to feline leukemia virus in the plasma of pet cats. | a method is described for determining levels of circulating immune complexes (cic) composed of feline leukemia virus (felv) antigens and corresponding antibodies in plasma of persistently-infected pet cats. the procedure is based on the ability of high-titered heterologous anti-felv serum to chase cat anti-felv igg from dissociated cic by successfully competing for binding of free antigen. the eluted cat antibody is then collected and quantitated. in a study of cats in the process of clearing pe ... | 1985 | 2995795 |
| antitumor activity of protein a administered intravenously to pet cats with leukemia or lymphosarcoma. | extracorporeal immunoadsorption with protein a (spa) containing staphylococcus aureus cowan i (sac) has previously been shown to induce an antitumor and antiviral response in some feline leukemia virus (felv)-infected, lymphosarcoma (lsa) cats. however, the mechanism by which this response is induced is unknown. since it is possible that spa dissociates from the sac column during treatment, and that intravenous infusion of spa could be a more efficacious form of treatment than extracorporeal imm ... | 1985 | 2983866 |
| feline leukemia virus-and feline sarcoma virus-related polypeptides released by virus producer and nonproducer cells. | polypeptides specific for feline leukemia virus (felv) have been identified in the media of cells that produce felv as well as in nonproducer cells transformed by feline sarcoma viruses (fesv). cat fibroblasts that were persistently infected with felv release the major virus envelope glycoprotein, whereas cultured cat lymphoma cells shed both glycopeptides related to the virus core gene (gag) and glycopeptides related to the virus envelope gene (env). mink cells and cat cells transformed by fesv ... | 1985 | 3002564 |
| [feline leukemia: current status regarding prevention]. | particularly as a result of the increasing popularity of keeping cats as pets, feline leukaemia constituted a risk to the cat population for many years; it was uncontrollable because of its insidious course and the spread of the virus, which passed unnoticed. during the past decade, diagnostic laboratory tests resulted in progress in the control of the infection for the first time. dissemination of the virus could then at least be checked to some extent. prevention by vaccination did not appear ... | 1986 | 3006279 |
| a feline retrovirus induced t-lymphoblastoid cell-line that produces an atypical alpha type of interferon. | a cell-line, designated lsa-1, was derived from a thymic lymphosarcoma that occurred in a cat with experimentally induced feline leukemia virus (felv) infection. lsa-1 cells possessed surface receptors and antigens of normal t-lymphocytes, but were unresponsive to interleukin-2 stimulation. the lsa cell-line was found to constitutively produce and release an interferon into the culture supernatants. production of this interferon was enhanced in certain clones of the original lsa-1 cell lines. th ... | 1986 | 3006326 |
| multilineage, non-species specific hematopoietic growth factor(s) elaborated by a feline fibroblast cell line: enhancement by virus infection. | in studies designed to determine the role of feline leukemia virus (felv) in the pathogenesis of marrow failure in the cat, we tested medium conditioned by uninfected and felv-infected feline embryonic fibroblasts (fea) for its effect on hematopoietic colony growth in culture. as opposed to an inhibitory effect, we found that the conditioned medium (cm) from fea or fea/felv increased the in vitro growth of multiple hematopoietic progenitor cell types including erythroid burst-forming cells (bfu- ... | 1986 | 3007539 |
| comments on evaluation of diagnostic test results. | | 1986 | 3015848 |
| a new acute transforming feline retrovirus with fms homology specifies a c-terminally truncated version of the c-fms protein that is different from sm-feline sarcoma virus v-fms protein. | the hz5-feline sarcoma virus (fesv) is a new acute transforming feline retrovirus which was isolated from a multicentric fibrosarcoma of a domestic cat. the hz5-fesv transforms fibroblasts in vitro and is replication defective. a biologically active integrated hz5-fesv provirus was molecularly cloned from cellular dna of hz5-fesv-infected fre-3a rat cells. the hz5-fesv has oncogene homology with the fms sequences of the sm-fesv. the genome organization of the 8.6-kilobase hz5-fesv provirus is 5' ... | 1986 | 3018286 |
| animal models: lessons from feline and bovine leukaemia virus infections. | | 1985 | 2989623 |
| lymphocytes and antibody in retrovirus-induced feline pure red cell aplasia. | the possible role of antibody and t-lymphocytes was investigated in the pure red cell aplasia (prca) associated with feline leukemia virus, subgroup c (felv-c), infection. in previous studies, erythroid colony-forming cells were undetectable in marrow culture of cats with prca. yet erythroid burst-forming cells (bfu-e) remained, suggesting that bfu-e were able to differentiate in vitro but not in vivo. it was inferred that immunologic suppression may contribute to the pathogenesis of feline prca ... | 1987 | 3025500 |
| retroviruses associated with leukemia and ablative syndromes in animals and in human beings. | t-lymphotropic retroviruses of cats cause lymphopenia and immunosuppression and represent the major cause of death in that species. similarly htlv-i which is t4 tropic is associated with an increased risk for development of infectious disease in regions where the virus is endemic. since htlv-i is also believed to be transmitted by blood and by sexual intercourse we considered the possibility that a variant form of htlv might cause aids. the identification of cross-reactive antibodies to htlv-i-m ... | 1985 | 2990682 |
| suppressive effect on polyclonal b-cell activation of a synthetic peptide homologous to a transmembrane component of oncogenic retroviruses. | purified feline leukemia virus, uv light-inactivated feline leukemia virus, and a synthetic peptide (cks-17) homologous to a well-conserved region of the transmembrane components of several human and animal retroviruses were each studied for their effects on igg production by feline peripheral blood lymphocytes. using a reverse hemolytic plaque assay, both the viable virus and the uv-inactivated feline leukemia virus, but not the cks-17, activated b lymphocytes to secrete igg. when staphylococca ... | 1987 | 3025858 |
| control of feline leukaemia virus infection by a removal programme. | | 1986 | 3026079 |
| retroviruses: leukemia and immunosuppression. the yohei ito memorial lecture. | | 1988 | 2904514 |
| feline glucose-6-phosphate dehydrogenase cellular mosaicism. application to the study of retrovirus-induced pure red cell aplasia. | neoplasms result from the uncontrolled proliferation of abnormal or transformed cells. the early stages of this process are difficult to study because of the lack of sensitive and specific markers of clonal evolution in an experimental system. we have developed a cat model using cellular mosaicism for glucose-6-phosphate dehydrogenase (g-6-pd). our findings confirm that the structural locus for feline g-6-pd is on the x-chromosome and demonstrate that it is randomly inactivated in somatic cells. ... | 1985 | 2981248 |
| suppression of in vitro neutrophil function by feline leukaemia virus (felv) and purified felv-p15e. | feline neutrophils (pmn) were isolated and exposed to ultraviolet light-inactivated feline leukaemia virus (uv-felv) and purified envelope component p15e (felv-p15e). functional capacity of exposed pmn was measured in vitro utilizing the chemiluminescence (cl) response. pmn exposed to uv-felv demonstrated depressed cl responses to ca2+-ionophore a23187 and latex particles. however, felv-p15e produced significant suppression in the cl response to a23187 but failed to produce significant alteratio ... | 1987 | 3029288 |
| retroviral transduction of t-cell antigen receptor beta-chain and myc genes. | support for multistage models of oncogenesis has been provided by several highly leukaemogenic retrovirus isolates that have transduced more than one host cell gene. where functional studies have been performed, these retroviral oncogenes show synergy for in vitro transformation and leukaemogenesis. in naturally occurring feline leukaemias associated with feline leukaemia virus (felv), retroviral transduction of myc is a frequent oncogenic mechanism. but evidence suggesting that the felv v-myc g ... | 1987 | 3029597 |
| [enzootic bovine leukosis and feline leukosis. 2 current animal models for the study of virus-induced lymphomas and immunodepression syndrome in man]. | | 1986 | 3030513 |
| [feline leukemia virus (felv) and felv-associated diseases in cats: a review]. | feline leukaemia virus (felv) usually occurs in its natural species, the domestic cat. felv is also important to human individuals as a comparative model, as felv may cause a variety of diseases which are partly malignant and partly benign, such as immunosuppression which bears a resemblance to aids (acquired immune deficiency syndrome) in man. although felv is a common infective agent, the incidence of disease due to felv is much higher in cats kept in closed households in which several of them ... | 1987 | 3039686 |
| transforming potential of a myc-containing variant of feline leukemia virus in vitro in early-passage feline cells. | we studied a naturally occurring variant of feline leukemia virus (felv) in which the oncogene myc has substituted for a portion of the viral structural genes (myc-felv). myc-felv was rescued by replication in the presence of felv as helper, and its biological activity was examined in early-passage feline cells in vitro. infection of leukocytes from peripheral blood, spleen, or thymus, or of kitten fibroblasts did not immortalize these cells or alter them morphologically. northern blot (rna blot ... | 1987 | 3041029 |
| chronic idiopathic neutropenia in a cat. | persistent neutropenia (0-0.6 x 10(9) neutrophils/l) was documented during a 10-month period in a 4-year-old spayed female domestic shorthair cat that was presented for anorexia and depression. salient abnormalities detected on physical examination were fever (40.3 degrees c), dehydration, and gingivitis. the cat was neutropenic (0.5 x 10(9) neutrophils/l) and enzyme-linked immunosorbent assay (elisa) test for feline leukemia virus was negative. a bone marrow aspirate showed decreased numbers of ... | 1988 | 3221355 |
| [aids--general characteristics and new clues in the studies of etiopathogenesis of the syndrome--the role of htlv]. | | 1985 | 3000090 |
| guinea pig erythrocyte rosette formation as a nonspecific cell surface receptor assay in the cat. | the specificity of guinea pig erythrocyte (gpe) rosettes for feline peripheral blood lymphocytes was studied. of the gpe rosette-positive cells from peripheral blood, 54% were monocytes, 29% were granulocytes, and only 17% were lymphocytes. results were similar for rosettes incubated at 4 c and those incubated at 37 c. mononuclear cells separated with polyvinylpyrrolidone-coated silica formed fewer monocyte rosettes (49%) and more granulocyte rosettes (34%) than did cells separated with sodium d ... | 1986 | 3954230 |
| a fluorescent antibody technique for the detection of the group-specific antigen of feline leukemia virus in infected tissue-culture cells. | | 1971 | 4105993 |
| histopathological and hematological findings in myeloid leukemia induced by a new feline leukemia virus isolate. | myeloid leukemia was induced by a new feline leukemia virus isolate felv-ab/gm-1 in a high proportion of cats. the latency period was short. three to 5 weeks after infection early changes were detectable in the bone marrow, and cats developed leukemia 5 to 8 weeks after infection. the results of the present histological and cytological studies suggested that there were two stages in the development of leukemia. the first stage appeared to be equivalent to the syndrome of bone marrow dysplasia or ... | 1986 | 3018983 |
| isolation of human amnion cells transformed by rescuable murine sarcoma virus. | | 1973 | 4350532 |
| immunology of oncornaviruses. | | 1974 | 4132209 |
| endogenous type c virus from a cat cell clone with properties distinct from previously described feline type c virus. | | 1973 | 4122424 |
| isolation of a t-lymphotropic virus from domestic cats with an immunodeficiency-like syndrome. | a highly t-lymphotropic virus was isolated from cats in a cattery in which all the animals were seronegative for feline leukemia virus. a number of cats in one pen had died and several had an immunodeficiency-like syndrome. only 1 of 18 normal cats in the cattery showed serologic evidence of infection with this new virus, whereas 10 of 25 cats with signs of ill health were seropositive for the virus. tentatively designated feline t-lymphotropic lentivirus, this new feline retrovirus appears to b ... | 1987 | 3643650 |
| review of viral disease of cats. | | 1972 | 4344966 |
| oncogenic activity of feline fibrosarcoma virus in newborn pigs. | | 1973 | 4348004 |
| trans-species rescue of defective genomes of murine sarcoma virus from hamster tumor cells with helper feline leukemia virus. | this report describes a trans-species rescue of defective msv genome with helper leukemia virus derived from cats. this rescue was achieved by in vitro co-cultivation of hamster tumor cells with feline embryo cells in the presence of helper feline leukemia virus (felv), or by inoculation of tumor cells into felv-infected newborn cats. the rescued focus-forming viruses produced foci in feline embryo cultures but not in cultures of mouse, rat, and hamster species. one isolate was tested and found ... | 1970 | 4313195 |
| human cancer in households containing cats with malignant lymphoma. | | 1972 | 4350192 |
| transformation by feline retroviruses. | | 1984 | 6099894 |
| [evidence for the existence of different antigenic determinants of the interspecies type in mammalian rna-c-type tumor viruses. comparative serological studies on viruses of various animal species including a virus suggested to be of human origin (author's transl)]. | | 1973 | 4131792 |
| differences in acute and convalescent-phase antibodies of cats infected with feline picornaviruses. | complement fixation inhibiting and complement-fixing antibodies were demonstrated in sera from cats during the acute and convalescent stages, respectively, of feline picornavirus infections. complement-fixing antibody activity was present in 19s and 7s globulins, whereas complement fixation inhibiting antibody activity was confined to the 7s globulins. sera with complement-fixing antibody and sera with complement fixation inhibiting antibody were also shown to have neutralizing and precipitating ... | 1974 | 4211925 |
| syncytial giant cell focus assay for viruses derived from feline leukemia and a simian sarcoma. | | 1973 | 4348580 |
| fucosylglycolipid metabolism in oncornavirus-transformed cell lines. | | 1973 | 4353889 |
| size differences in the ribonucleic acids of feline leukaemia viruses. | | 1973 | 4357376 |
| rd 114 virus-specific sequences in feline cellular rna: detection and characterization. | rna extracted from cat cells contains sequences homologous to rd-114 viral rna. the sequences are measured by molecular hybridization with a single-stranded dna probe synthesized by the virion polymerase using the endogenous viral rna as template. viral-specific rna has been detected in all cells of cat origin tested thus far, but not in cells of other animals, except for the virus-producing human rhabdomyosarcoma cell, rd-114. the extent of hybridization of the dna probe to cellular rna was equ ... | 1973 | 4358169 |
| feline leukemia. | | 1980 | 6251017 |
| electron microscopy of feline leukaemia virus rna. | | 1973 | 4360153 |
| 3'-azido-3'-deoxythymidine in feline leukemia virus-infected cats: a model for therapy and prophylaxis of aids. | due to similarities between human immunodeficiency virus and feline leukemia virus, the etiological agents of acquired immunodeficiency syndromes in humans and cats, the feline system was used as a model to conduct preliminary investigations as to the efficacy of the thymidine analogue 3'-azido-3'-deoxythymidine (azt) as a therapeutic and preventive agent against retroviruses. in vitro evaluations of azt cytotoxicity and its antiviral effects were conducted. subsequently, 50 6-week-old specific ... | 1987 | 3034403 |
| prevalence of feline leukemia virus infection in random source laboratory cats. | three years of quarantine data involving the prevalence of feline leukemia virus (felv) in laboratory cats (felis catus) was evaluated. testing was performed using a commercially available elisa system. annual prevalence of infection ranged from 5.4 to 10.7% of the 937 cats tested. results obtained with the enzyme linked immunosorbent assay (elisa) system compared well with tests performed using an immunofluorescent antibody assay system (ifa). seasonal periodicity was noted with higher infectio ... | 1987 | 3039250 |
| interspecies infection by feline leukemia virus: serial cell-free transmission in dogs of malignant lymphomas induced by feline leukemia virus. | | 1973 | 4360147 |
| significance of leukemia in animals for the human disease. | | 1973 | 4360150 |
| simple, quantitative assay for both xenotropic murine leukemia and ecotropic feline leukemia viruses. | a cat cell line carrying the genome of a murine sarcoma virus developed discrete foci in linear response to infection with feline leukemia virus or xenotropic murine c-type virus. | 1974 | 4365331 |
| editorial: cat leukemia: a threat to man? | | 1974 | 4359320 |
| editorial: the current status of feline leukemia virus research. | | 1974 | 4374332 |
| feline malignant mammary tumors. ii. immunologic and electron microscopic investigations into a possible viral etiology. | | 1974 | 4363687 |