Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| molecular detection of hepatozoon spp. and cytauxzoon sp. in domestic and stray cats from madrid, spain. | different species of apicomplexan protozoans of the genera hepatozoon and cytauxzoon can infect domestic cats, but their epidemiology and clinical relevance are not fully understood. the aim of this study was to assess the molecular prevalence of hepatozoon spp. and cytauxzoon spp. and to identify associated risk factors and clinical and laboratory abnormalities in a population of cats from madrid, spain. | 2017 | 28285590 |
| presence of a shared 5'-leader sequence in ancestral human and mammalian retroviruses and its transduction into feline leukemia virus. | recombination events induce significant genetic changes, and this process can result in virus genetic diversity or in the generation of novel pathogenicity. we discovered a new recombinant feline leukemia virus (felv) gag gene harboring an unrelated insertion, termed the "x-region", which was derived from fcerv-gamma4, a feline endogenous gammaretrovirus. the identified fcerv-gamma4 proviruses have lost their coding capability, but some can express their viral rna in feline tissues. although the ... | 2017 | 28768854 |
| molecular detection of viral agents in free-ranging and captive neotropical felids in brazil. | we describe molecular testing for felid alphaherpesvirus 1 (fhv-1), carnivore protoparvovirus 1 (cppv-1), feline calicivirus (fcv), alphacoronavirus 1 (feline coronavirus [fcov]), feline leukemia virus (felv), feline immunodeficiency virus (fiv), and canine distemper virus (cdv) in whole blood samples of 109 free-ranging and 68 captive neotropical felids from brazil. samples from 2 jaguars ( panthera onca) and 1 oncilla ( leopardus tigrinus) were positive for fhv-1; 2 jaguars, 1 puma ( puma conc ... | 2017 | 28677421 |
| host and viral traits predict zoonotic spillover from mammals. | the majority of human emerging infectious diseases are zoonotic, with viruses that originate in wild mammals of particular concern (for example, hiv, ebola and sars). understanding patterns of viral diversity in wildlife and determinants of successful cross-species transmission, or spillover, are therefore key goals for pandemic surveillance programs. however, few analytical tools exist to identify which host species are likely to harbour the next human virus, or which viruses can cross species ... | 2017 | 28636590 |
| fatal toxoplasmosis in an immunosuppressed domestic cat from brazil caused by toxoplasma gondii clonal type i. | the objective of the study was to report on a fatal case of feline toxoplasmosis with coinfection with the feline leukemia virus (felv). a domestic cat (felis silvestris catus) presented intense dyspnea and died three days later. in the necropsy, the lungs were firm, without collapse and with many white areas; moderate lymphadenomegaly and splenomegaly were also observed. the histopathological examination showed severe necrotic interstitial bronchopneumonia and mild necrotic hepatitis, associate ... | 2017 | 28746448 |
| epidemiologic survey of feline leukemia virus in domestic cats on tsushima island, japan: management strategy for tsushima leopard cats. | the tsushima leopard cat (tlc) prionailurus bengalensis euptilurus, a subspecies of p. bengalensis, is designated a national natural monument of japan, and lives only on tsushima island, nagasaki prefecture, japan. tlcs are threatened by various infectious diseases. feline leukemia virus (felv) causes a serious infectious disease with a poor prognosis in cats. therefore, the transmission of felv from tsushima domestic cats (tdcs) to tlcs may threaten the tlc population. we investigated the felv ... | 2017 | 28782421 |
| viral diagnostic criteria for feline immunodeficiency virus and feline leukemia virus infections in domestic cats from buenos aires, argentina. | a cross-sectional study was carried out on cats attending the small animal hospital at the faculty of veterinary sciences of the university of buenos aires to assess the prevalence and associated risk factors of feline immunodeficiency virus (fiv) and feline leukemia virus (felv) in the city of buenos aires, argentina. blood samples from 255 cats with symptoms compatible with fiv or felv infection, collected between 2009 and 2013 were analyzed by serology (immunochromatography, ia) and by hemi-n ... | 2016 | 27825735 |
| suspected myelodysplastic/myeloproliferative neoplasm in a feline leukemia virus-negative cat. | a 10-year-old castrated domestic short-haired cat was presented to a primary care veterinarian for a wellness examination and laboratory examination for monitoring of diabetes mellitus. the cbc revealed marked thrombocytosis, leukopenia and macrocytic, normochromic anemia. the cat tested negative for felv and feline immunodeficiency virus, but was positive for mycoplasma haemominutum by pcr. hematologic abnormalities were not responsive to therapy, so a repeat cbc and a bone marrow aspiration fo ... | 2016 | 27870069 |
| role of feline immunodeficiency virus in lymphomagenesis--going alone or colluding? | feline immunodeficiency virus (fiv) is a naturally occurring lentivirus of domestic and nondomestic feline species. infection in domestic cats leads to immune dysfunction via mechanisms similar to those caused by human immunodeficiency virus (hiv) and, as such, is a valuable natural animal model for acquired immunodeficiency syndrome (aids) in humans. an association between fiv and an increased incidence of neoplasia has long been recognized, with frequencies of up to 20% in fiv-positive cats re ... | 2016 | 27034392 |
| toxoplasma gondii, dirofilaria immitis, feline immunodeficiency virus (fiv), and feline leukemia virus (felv) infections in stray and pet cats (felis catus) in northwest china: co-infections and risk factors. | this study was conducted to estimate the prevalence of toxoplasma gondii, dirofilaria immitis, feline immunodeficiency virus (fiv), and feline leukemia virus (felv) infections among stray and pet cats in lanzhou, northwest china, and to identify the influence of age, gender, and regions on seropositivity. t. gondii antibodies were examined in cat sera by the modified agglutination test (mat). the circulating antigens of d. immitis and felv and specific antibodies to fiv were examined using kits ... | 2016 | 26362646 |
| detection of leishmania major and leishmania tropica in domestic cats in the ege region of turkey. | leishmaniosis is a group of diseases caused by different species of leishmania parasites in mammalian species. the aim of the present study was to investigate the presence of leishmania spp. dna in cats using real time polymerase chain reaction (rt-pcr) assays targeting internal transcribed spacer (its1) and heat-shock protein 70 gene (hsp70) regions with leishmania species-specific primers and probes. blood samples were collected from 147 cats (73 female; 74 male) in the endemic regions for zoo ... | 2015 | 26277567 |
| feline immunodeficiency virus and feline leukemia virus infection in free-ranging guignas (leopardus guigna) and sympatric domestic cats in human perturbed landscapes on chiloé island, chile. | feline immunodeficiency virus (fiv) and feline leukemia virus (felv) are two of the most common viruses affecting domestic cats (felis catus). during the last two decades, reports show that both viruses also infect or affect other species of the family felidae. human landscape perturbation is one of the main causes of emerging diseases in wild animals, facilitating contact and transmission of pathogens between domestic and wild animals. we investigated fiv and felv infection in free-ranging guig ... | 2015 | 25380363 |
| retroviral dna--the silent winner: blood transfusion containing latent feline leukemia provirus causes infection and disease in naïve recipient cats. | the feline leukemia virus (felv) is a gamma-retrovirus of domestic cats that was discovered half a century ago. cats that are infected with felv may develop a progressive infection resulting in persistent viremia, immunodeficiency, tumors, anemia and death. a significant number of cats mount a protective immune response that suppresses viremia; these cats develop a regressive infection characterized by the absence of viral replication and the presence of low levels of proviral dna. the biologica ... | 2015 | 26689419 |
| a soluble envelope protein of endogenous retrovirus (felix) present in serum of domestic cats mediates infection of a pathogenic variant of feline leukemia virus. | t-lymphotropic feline leukemia virus (felv-t), a highly pathogenic variant of felv, induces severe immunosuppression in cats. felv-t is fusion defective because in its phq motif, a gammaretroviral consensus motif in the n terminus of an envelope protein, histidine is replaced with aspartate. infection by felv-t requires felix, a truncated envelope protein encoded by an endogenous felv, for transactivation of infectivity and pit1 for binding felix. although pit1 is present in most tissues in cats ... | 2015 | 25395593 |
| gammaretrovirus-specific antibodies in free-ranging and captive namibian cheetahs. | the cheetah population in namibia is the largest free-ranging population in the world and a key population for research regarding the health status of this species. we used serological methods and quantitative real-time pcr to test free-ranging and captive namibian cheetahs for the presence of feline leukemia virus (felv), a gammaretrovirus that can be highly aggressive in populations with low genetic diversity, such as cheetahs. we also assessed the presence of antibodies to other gammaretrovir ... | 2015 | 25809630 |
| ancestral mutations acquired in refrex-1, a restriction factor against feline retroviruses, during its cooption and domestication. | endogenous retroviruses (ervs) are remnants of ancestral retroviral infections of germ cells. retroviral endogenization is an adaptation process for the host genome, and ervs are gradually attenuated or inactivated by mutation. however, some ervs that have been "domesticated" by their hosts eventually gain physiological functions, such as placentation or viral resistance. we previously reported the discovery of refrex-1, a soluble antiretroviral factor in domestic cats that specifically inhibits ... | 2015 | 26581999 |
| overexpression of feline tripartite motif-containing 25 interferes with the late stage of feline leukemia virus replication. | tripartite motif-containing 25 (trim25) regulates various cellular processes through e3 ubiquitin ligase activity. previous studies have revealed that the expression of trim25 is induced by type i interferon and that trim25 is involved in the host cellular innate immune response against retroviral infection. although retroviral infection is prevalent in domestic cats, the roles of feline trim25 in the immune response against these viral infections are poorly understood. because feline trim25 is ... | 2015 | 25913257 |
| annotated features of domestic cat - felis catus genome. | domestic cats enjoy an extensive veterinary medical surveillance which has described nearly 250 genetic diseases analogous to human disorders. feline infectious agents offer powerful natural models of deadly human diseases, which include feline immunodeficiency virus, feline sarcoma virus and feline leukemia virus. a rich veterinary literature of feline disease pathogenesis and the demonstration of a highly conserved ancestral mammal genome organization make the cat genome annotation a highly in ... | 2014 | 25143822 |
| analysis of single-nucleotide polymorphisms in the apobec3h gene of domestic cats (felis catus) and their association with the susceptibility to feline immunodeficiency virus and feline leukemia virus infections. | feline immunodeficiency virus (fiv) and feline leukemia virus (felv) are widely distributed retroviruses that infect domestic cats (felis catus). restriction factors are proteins that have the ability to hamper retroviruses' replication and are part of the conserved mechanisms of anti-viral immunity of mammals. the apobec3 protein family is the most studied class of restriction factors; they are cytidine deaminases that generate hypermutations in provirus dna during reverse transcription, thus c ... | 2014 | 25173082 |
| seroprevalence of feline leukemia virus, feline immunodeficiency virus and heartworm infection among owned cats in tropical mexico. | several infectious agents may be distributed within a healthy population of cats where diverse risk factors predispose them to come into contact with pathogens. blood samples from 227 owned cats in merida, mexico, were collected with the objective of determining the seroprevalence and associated risk factors of feline leukemia virus (felv) and dirofilaria immitis antigen, and feline immunodeficiency virus (fiv) antibody. serological detection of felv and d immitis antigens, and fiv antibodies wa ... | 2014 | 24196568 |
| detection of serum antibodies against bartonella species in cats with sporotrichosis from rio de janeiro, brazil. | cat scratch disease is a zoonosis caused by bartonella species, transmitted to humans through scratches or bites from infected cats and via direct contact with infected feces. sporotrichosis, caused by the fungal complex sporothrix, is transmitted by traumatic inoculation of the fungus. cats are important in zoonotic transmission. serum samples from 112 domestic cats with sporotrichosis and 77 samples from healthy cats were analyzed by indirect immunofluorescence assay (ifa), using the commercia ... | 2014 | 24127458 |
| serosurvey for selected viral infections in free-ranging jaguars (panthera onca) and domestic carnivores in brazilian cerrado, pantanal, and amazon. | we investigated the exposure of jaguar (panthera onca) populations and domestic carnivores to selected viral infections in the cerrado, amazon, and pantanal biomes of brazil. between february 2000 and january 2010, we collected serum samples from 31 jaguars, 174 dogs (canis lupus familiaris), and 35 domestic cats (felis catus). serologic analyses for antibodies to rabies virus, canine distemper virus (cdv), feline immunodeficiency virus (fiv), and for feline leukemia virus (felv) antigen were co ... | 2013 | 23778599 |
| refrex-1, a soluble restriction factor against feline endogenous and exogenous retroviruses. | the host defense against viral infection is acquired during the coevolution or symbiosis of the host and pathogen. several cellular factors that restrict retroviral infection have been identified in the hosts. feline leukemia virus (felv) is a gammaretrovirus that is classified into several receptor interference groups, including a novel felv-subgroup d (felv-d) that we recently identified. felv-d is generated by transduction of the env gene of feline endogenous gammaretrovirus of the domestic c ... | 2013 | 23966402 |
| the surface glycoprotein of feline leukemia virus isolate felv-945 is a determinant of altered pathogenesis in the presence or absence of the unique viral long terminal repeat. | feline leukemia virus (felv) is a naturally transmitted gammaretrovirus that infects domestic cats. felv-945, the predominant isolate associated with non-t-cell disease in a natural cohort, is a member of felv subgroup a but differs in sequence from the felv-a prototype, felv-a/61e, in the surface glycoprotein (su) and long terminal repeat (ltr). substitution of the felv-945 ltr into felv-a/61e resulted in pathogenesis indistinguishable from that of felv-a/61e, namely, thymic lymphoma of t-cell ... | 2013 | 23903838 |
| prevalence of feline leukemia virus infection in domestic cats in rio de janeiro. | peripheral blood smears of 1094 domestic cats were collected and tested by indirect immunofluorescence antibody assay for p27 antigen in cells to study the prevalence and risk factors for feline leukemia virus (felv) in the state of rio de janeiro. sex, age, breed, outdoor access, neutering status, type of habitation (household, shelter, veterinary clinics and other places), number of household cats and clinical signs were registered on a form. among the tested samples, 11.52% were positive. ris ... | 2012 | 22492347 |
| clinical aspects of feline retroviruses: a review. | feline leukemia virus (felv) and feline immunodeficiency virus (fiv) are retroviruses with global impact on the health of domestic cats. the two viruses differ in their potential to cause disease. felv is more pathogenic, and was long considered to be responsible for more clinical syndromes than any other agent in cats. felv can cause tumors (mainly lymphoma), bone marrow suppression syndromes (mainly anemia), and lead to secondary infectious diseases caused by suppressive effects of the virus o ... | 2012 | 23202500 |
| virological survey in free-ranging wildcats (felis silvestris) and feral domestic cats in portugal. | to determine the presence of viral pathogens in natural areas a survey was conducted on an opportunistic sample of fifty eight wild (felis silvestris silvestris) and feral cats (f. s. catus). the biological materials included serum, lung tissue extract and stool. feline leukemia virus p27 antigen was detected in 13/50 serum/lung tissue extract samples (26%), canine distemper virus antibodies were detected in 2/26 serum/lung tissue extract samples (7.7%), feline coronavirus rna was present in 6/2 ... | 2012 | 22424865 |
| cryptosporidium species screening using kinyoun technique in domestic cats with diarrhea. | cryptosporidium is a coccidian that can lead to diarrhea, especially in immunosuppressed individuals. retroviruses are considered a primary cause of immunosuppression in cats. fecal specimens and blood collected from the 60 cats were evaluated for the presence of acid-fast cryptosporidia in three consecutive stool samples and for feline leukemia virus (felv) antigen and feline immunodeficiency virus (fiv) antibody by elisa testing. five animals (8.33%) shedding oocysts were found, one was both f ... | 2012 | 22314086 |
| disruption of thiamine uptake and growth of cells by feline leukemia virus subgroup a. | feline leukemia virus (felv) is still a major cause of morbidity and mortality in domestic cats and some wild cats despite the availability of relatively effective vaccines against the virus. felv subgroup a (felv-a) is transmitted in natural infections, and felv subgroups b, c, and t can evolve directly from felv-a by mutation and/or recombination with endogenous retroviruses in domestic cats, resulting in a variety of pathogenic outcomes. the cell surface entry receptor for felv-a is a putativ ... | 2012 | 23269813 |
| emerging viruses in the felidae: shifting paradigms. | the domestic cat is afflicted with multiple viruses that serve as powerful models for human disease including cancers, sars and hiv/aids. cat viruses that cause these diseases have been studied for decades revealing detailed insight concerning transmission, virulence, origins and pathogenesis. here we review recent genetic advances that have questioned traditional wisdom regarding the origins of virulent feline infectious peritonitis (fip) diseases, the pathogenic potential of feline immunodefic ... | 2012 | 22470834 |
| quantification of the humoral immune response and hemoplasma blood and tissue loads in cats coinfected with 'candidatus mycoplasma haemominutum' and feline leukemia virus. | 'candidatus mycoplasma haemominutum' (cmhm) is a hemotropic mycoplasma (aka hemoplasma) of domestic cats and wild felids. in a transmission study, we exposed eight specified pathogen-free cats to blood from iberian lynxes (lynx pardinus) infected with cmhm. the cats were coinfected with feline leukemia virus (felv) from an iberian lynx or with a prototype felv. the goal of the present study was to quantify the humoral immune response to cmhm and to identify potential target tissues and sequestra ... | 2012 | 22588083 |
| survey of the feline leukemia virus infection status of cats in southern germany. | most studies that investigate the prevalence of infections with feline leukemia virus (felv) are based on the detection of p27 antigen in blood, but they do not detect proviral dna to identify the prevalence of regressive felv infections. the aim of the present study was to assess the prevalence and status of felv infection in cats in southern germany. p27 antigen enzyme-linked immunosorbent assay (elisa), anti-p45 antibody elisa, dna polymerase chain reaction (pcr) of blood and rna pcr of saliv ... | 2012 | 22403413 |
| infectious endogenous retroviruses in cats and emergence of recombinant viruses. | endogenous retroviruses (ervs) comprise a significant percentage of the mammalian genome, and it is poorly understood whether they will remain as inactive genomes or emerge as infectious retroviruses. although several types of ervs are present in domestic cats, infectious ervs have not been demonstrated. here, we report a previously uncharacterized class of endogenous gammaretroviruses, termed erv-dcs, that is present and hereditary in the domestic cat genome. we have characterized a subset of e ... | 2012 | 22674983 |
| exposure to selected pathogens in to selected pathogens in geoffroy's cats and domestic carnivores from central argentina. | wild carnivores share a high percentage of parasites and viruses with closely related domestic carnivores. because of increased overlap and potential contact with domestic species, we conducted a retrospective serosurvey for 11 common carnivore pathogens in 40 geoffroy's cats (leopardus geoffroyi) sampled between 2000 and 2008 within or near two protected areas in central argentina (lihué calel national park, la pampa, and campos del tuyú national park, buenos aires), as well as five domestic ca ... | 2012 | 23060491 |
| are endogenous feline leukemia viruses really endogenous? | full length endogenous feline leukemia virus (felv) proviruses exist within the genomes of many breeds of domestic cat raising the possibility that they may also exist in a transmissible exogenous form. such viruses would share receptor usage with the recombinant felv-b subgroup, a viral subgroup that arises in vivo by recombination between exogenous subgroup a virus (felv-a) and endogenous felv. accordingly, all isolates of felv-b made to date have contained a "helper" felv-a, consistent with t ... | 2011 | 21715022 |
| Surveillance using serological and molecular methods for the detection of infectious agents in captive Brazilian neotropic and exotic felids. | The aim of the current study was to investigate the exposure of captive wild felids to various infectious pathogens using serological and molecular methods. One hundred and fifty-nine neotropic felids and 51 exotic felids from 28 captive settings in Brazil were tested. While antibodies against Feline parvovirus and Feline coronavirus (FCoV), Feline calicivirus and Bartonella spp. were frequently detected by serologic tests, antibodies against Felid herpesvirus 1 or infection with hemotropic myco ... | 2011 | 21908268 |
| Feline leukemia virus infection requires a post-receptor binding envelope-dependent cellular component. | Gammaretrovirus receptors have been suggested to contain the necessary determinants to mediate virus binding and entry. Here, we show that murine NIH 3T3 and baby hamster kidney (BHK) cells overexpressing receptors for subgroup A, B, and C feline leukemia viruses (FeLVs) are weakly susceptible (10(1) to 10(2) CFU/ml) to FeLV pseudotype viruses containing murine leukemia virus (MLV) core (Gag-Pol) proteins, whereas FeLV receptor-expressing murine Mus dunni tail fibroblast (MDTF) cells are highly ... | 2011 | 21917946 |
| Viral determinants of FeLV infection and pathogenesis: lessons learned from analysis of a natural cohort. | Detailed analysis has been performed over many years of a geographic and temporal cohort of cats naturally infected with feline leukemia virus (FeLV). Molecular analysis of FeLV present in the diseased tissues and application of those viruses to experimental systems has revealed unique isolates with distinctive disease potential, previously uncharacterized virus-receptor interactions, information about the role of recombinant viruses in disease induction, and novel viral and cellular oncogenes i ... | 2011 | 21994802 |
| inhibition of feline leukemia virus replication by the integrase inhibitor raltegravir. | the oncogenic gammaretrovirus feline leukemia virus (felv) has been the leading cause of death among domestic cats until the introduction of efficient diagnostics and vaccines in the late 1980s. so far, no efficient treatment for viremic animals is available. hence, use of the felv model to evaluate antiretroviral therapies applied to hiv is a timely task. the efficacy of the integrase inhibitor raltegravir, which is widely used for the treatment of hiv in humans, has been assessed in vitro for ... | 2011 | 21570781 |
| seroepidemiology of toxoplasma gondii, neospora caninum, and leishmania spp. infections and risk factors for cats from brazil. | the seroprevalence of infection by toxoplasma gondii, neospora caninum, and leishmania spp. was detected through an indirect immunofluorescence in 70 cats from the andradina municipality, são paulo state, brazil. anti-t. gondii antibodies (titer >64) were detected in 15.7% (11/70) of animals, whereas positivity for n. caninum (titer 16) was not observed in any animal. of the cats from urban and rural areas, 10.4% (5/48) and 27.2% (6/22) were positive for t. gondii, respectively. breed, age, food ... | 2011 | 21626423 |
| assessment of feline blood for transfusion purposes in the dublin area of ireland. | the prevalence of a, b and ab blood types and of feline immunodeficiency virus (fiv) and feline leukaemia virus (felv) infection was determined in cats in ireland, in order to determine risk factors for blood taken for transfusion purposes. edta blood samples were available from 137 non-pedigree cats and 39 pedigree cats (91 females and 85 males, aged four months to 15.0 years) in the dublin area of ireland. of the 176 edta blood samples obtained, 112 (from 92 healthy cats and 20 sick cats) were ... | 2011 | 21498236 |
| the surface glycoprotein of a natural feline leukemia virus subgroup a variant, felv-945, as a determinant of disease outcome. | feline leukemia virus (felv) is a natural retrovirus of domestic cats associated with degenerative, proliferative and malignant diseases. studies of felv infection in a cohort of naturally infected cats were undertaken to examine felv variation, the selective pressures operative in felv infection that lead to predominance of natural variants, and the consequences for infection and disease progression. a unique variant, designated felv-945, was identified as the predominant isolate in the cohort ... | 2011 | 21764142 |
| a serological and molecular study of leishmania infantum infection in cats from the island of ibiza (spain). | the aim of this study was to evaluate the prevalence of leishmania infantum infection within a feline population by serologic and molecular methods and to identify associated risk factors. one hundred five cats living outdoors were studied. sera were tested for igg antibodies against l. infantum, toxoplasma gondii, and feline immunodeficiency virus (fiv) and for the detection of feline leukemia virus (felv) p27 antigen by enzyme-linked immunosorbent assay (elisa). l. infantum real-time polymeras ... | 2011 | 20804432 |
| clinical aspects of feline immunodeficiency and feline leukemia virus infection. | feline leukemia virus (felv) and feline immunodeficiency virus (fiv) are retroviruses with a global impact on the health of domestic cats. the two viruses differ in their potential to cause disease. fiv can cause an acquired immunodeficiency syndrome that increases the risk of developing opportunistic infections, neurological diseases, and tumors. in most naturally infected cats, however, fiv itself does not cause severe clinical signs, and fiv-infected cats may live many years without any healt ... | 2011 | 21807418 |
| isolation and genotyping of toxoplasma gondii causing fatal systemic toxoplasmosis in an immunocompetent 10-year-old cat. | a 10-year-old male, neutered domestic shorthair cat was presented with fever, anorexia, vomiting, and diarrhea. serologic testing for feline immunodeficiency virus and feline leukemia virus were negative. fine-needle aspirates of mesenteric lymph nodes revealed the presence of banana-shaped apicomplexan parasites. the cat died after 4 days of hospitalization. postmortem polymerase chain reaction (pcr) analysis confirmed the presence of toxoplasma gondii in all examined organs. parasites were ex ... | 2011 | 21217037 |
| establishment of a novel feline leukemia virus (felv)-negative b-cell cell line from a cat with b-cell lymphoma. | we established a novel feline b-cell line, ms4, from the neoplastic pleural effusion of a cat with cutaneous b-cell lymphoma. immunophenotype staining of the ms4 cells was positive for cd20, cd79a, and iga and negative for cd3, cd4, cd5, cd8a, cd18, cd21, cd22, igm, igg, ig light chain, and mhc class ii. pcr analysis for immunoglobulin heavy chain gene rearrangements revealed a monoclonal rearrangement, whereas no clonal rearrangement of the t-cell receptor ? gene was detected. southern blotting ... | 2011 | 21272942 |
| [FeLV infection in the cat: clinically relevant aspects]. | The feline leukemia virus (FeLV) is a retrovirus of the domestic cat that was described almost 50 years ago. The FeLV-infection may lead to fatal diseases in domestic and small wild cats. The use of efficacious diagnostics assays and vaccines led to a reduction of the FeLV prevalence; however, FeLV still poses a problem for the cat presented with the infection. This article aims to describe recent developments in diagnostics and findings in the infection pathogenesis that are clinically relevant ... | 2011 | 22045454 |
| when cats' ways of life interact with their viruses: a study in 15 natural populations of owned and unowned cats (felis silvestris catus). | in natural populations, virus circulation is influenced by host behavior and physiological characteristics. cat populations exhibit a great variability in social and spatial structure, the existence of different ways of life within a same population may also result in different epidemiological patterns. to test this hypothesis, we used a logistic regression to analyze the risk factors of feline immunodeficiency virus (fiv), feline herpes virus (fhv), feline calicivirus (fcv), and feline parvovir ... | 2011 | 21705099 |
| quantification and molecular characterization of the feline leukemia virus a receptor. | virus receptors and their expression patterns on the cell surface determine the cell tropism of the virus, host susceptibility and the pathogenesis of the infection. feline thiamine transport protein 1 (fthtr1) has been identified as the receptor for feline leukemia virus (felv) a. the goal of the present study was to develop a quantitative, taqman real-time pcr assay to investigate fthtr1 mrna expression in tissues of uninfected and felv-infected cats, cats of different ages, in tumor tissues a ... | 2011 | 21889617 |
| feline tetherin efficiently restricts release of feline immunodeficiency virus but not spreading of infection. | domestic cats endure infections by all three subfamilies of the retroviridae: lentiviruses (feline immunodeficiency virus [fiv]), gammaretroviruses (feline leukemia virus [felv]), and spumaretroviruses (feline foamy virus [ffv]). thus, cats present an insight into the evolution of the host-retrovirus relationship and the development of intrinsic/innate immune mechanisms. tetherin (bst-2) is an interferon-inducible transmembrane protein that inhibits the release of enveloped viruses from infected ... | 2011 | 21490095 |
| seroprevalence of toxoplasma gondii and concurrent bartonella spp., feline immunodeficiency virus, feline leukemia virus, and dirofilaria immitis infections in egyptian cats. | abstract toxoplasma gondii and bartonella spp. are zoonotic pathogens of cats. feline immunodeficiency virus (fiv) and feline leukemia virus (felv) are related to human immunodeficiency virus and human leukemia virus, respectively, and these viruses are immunosuppressive. in the present study, the prevalence of antibodies to t. gondii , bartonella spp., fiv, as well as felv and dirofilaria immitis antigens was determined in sera from feral cats ( felis catus ) from cairo, egypt. using a modified ... | 2011 | 21506874 |
| protection against feline leukemia virus challenge for at least 2 years after vaccination with an inactivated feline leukemia virus vaccine. | twelve cats were vaccinated at 8 and 11 weeks of age with a commercially available inactivated felv vaccine (nobivac felv, intervet/schering-plough animal health). eleven cats served as age-matched, placebo-vaccinated controls. all cats were kept in isolation for 2 years after vaccination and were then challenged with virulent felv to evaluate vaccine efficacy and duration of immunity. cats were monitored for 12 weeks after challenge for development of persistent viremia using a commercial felv ... | 2010 | 20957616 |
| encephalomyelitis resembling human and ruminant rhombencephalitis caused by listeria monocytogenes in a feline leukemia virus-infected cat. | 2010 | 20412434 | |
| polymerase chain reaction survey of feline haemoplasma infections in greece. | the aim of this study was to use real-time polymerase chain reaction assays to determine the prevalence of three haemoplasma species in cats from greece and to evaluate possible associations between haemoplasma infection and age, gender, feline immunodeficiency virus/feline leukaemia virus (fiv/felv) status and packed cell volume (pcv). ninety-seven cats (24 ill anaemic, 55 ill non-anaemic, 18 healthy non-anaemic) were included in the study. twenty cats (20.6%) were haemoplasma positive; seven c ... | 2010 | 20580298 |
| malignant lymphoma in african lions (panthera leo). | malignant lymphoma has become an increasingly recognized problem in african lions (panthera leo). eleven african lions (9 male and 2 female) with clinical signs and gross and microscopic lesions of malignant lymphoma were evaluated in this study. all animals were older adults, ranging in age from 14 to 19 years. immunohistochemically, 10 of the 11 lions had t-cell lymphomas (cd3(+), cd79a(-)), and 1 lion was diagnosed with a b-cell lymphoma (cd3(-), cd79a(+)). the spleen appeared to be the prima ... | 2010 | 20610770 |
| the frequency of occurrence and nature of recombinant feline leukemia viruses in the induction of multicentric lymphoma by infection of the domestic cat with felv-945. | during feline leukemia virus (felv) infection in the domestic cat, viruses with a novel envelope gene arise by recombination between endogenous felv-related elements and the exogenous infecting species. these recombinant viruses (felv-b) are of uncertain disease association, but have been linked to the induction of thymic lymphoma. to assess the role of felv-b in the induction of multicentric lymphoma and other non-t-cell disease, the frequency of occurrence and nature of felv-b were examined in ... | 2010 | 20451235 |
| light whole genome sequence for snp discovery across domestic cat breeds. | the domestic cat has offered enormous genomic potential in the veterinary description of over 250 hereditary disease models as well as the occurrence of several deadly feline viruses (feline leukemia virus--felv, feline coronavirus--fecv, feline immunodeficiency virus--fiv) that are homologues to human scourges (cancer, sars, and aids respectively). however, to realize this bio-medical potential, a high density single nucleotide polymorphism (snp) map is required in order to accomplish disease a ... | 2010 | 20576142 |
| does a feline leukemia virus infection pave the way for bartonella henselae infection in cats? | domestic cats serve as the reservoir hosts of bartonella henselae and may develop mild clinical symptoms or none after experimental infection. in humans, b. henselae infection can result in self-limiting cat scratch disease. however, immunocompromised patients may suffer from more-severe courses of infection or may even develop the potentially lethal disease bacillary angiomatosis. it was reasoned that cats with immunocompromising viral infections may react similarly to b. henselae infection. th ... | 2010 | 20610682 |
| evolutionary dynamics of endogenous feline leukemia virus proliferation among species of the domestic cat lineage. | endogenous feline leukemia viruses (enfelvs) occur in the germ lines of the domestic cat and related wild species (genus felis). we sequenced the long terminal repeats and part of the env region of enfelvs in domestic cats and five wild species. a total of 305 enfelv sequences were generated across 17 individuals, demonstrating considerable diversity within two major clades. distinct proliferations of enfelvs occurred before and after the black-footed cat diverged from the other species. diversi ... | 2010 | 20615520 |
| incidence of persistent viraemia and latent feline leukaemia virus infection in cats with lymphoma. | in the past, feline leukaemia virus (felv) infection, and also latent felv infection, were commonly associated with lymphoma and leukaemia. in this study, the prevalence of felv provirus in tumour tissue and bone marrow in felv antigen-negative cats with these tumours was assessed. seventy-seven diseased cats were surveyed (61 antigen-negative, 16 antigen-positive). blood, bone marrow, and tumour samples were investigated by two polymerase chain reaction (pcr) assays detecting deoxyribonucleic a ... | 2010 | 21131219 |
| exposure of cats to low doses of felv: seroconversion as the sole parameter of infection. | in felids, feline leukemia virus (felv) infection results in a variety of outcomes that range from abortive (virus readily eliminated and never detectable) to progressive infection (persistent viremia and viral shedding). recently, a novel outcome was postulated for low felv infectious doses. naïve cats exposed to faeces of persistently infected cats seroconverted, indicating infection, but remained negative for provirus and p27 antigen in blood. felv provirus was found in some tissues but not i ... | 2010 | 19861115 |
| feline leukemia virus infection: a threat for the survival of the critically endangered iberian lynx (lynx pardinus). | the iberian lynx (lynx pardinus) is considered the most endangered felid species in the world. to date, less than 200 animals remain in the wild. low numbers and genetic uniformity may contribute to render this species particularly susceptible to infectious diseases. different pathogens have been identified in iberian lynxes; including several feline bacterial and viral agents. within a 6-month period starting in december 2006, 12 lynxes living in the northern part of the doñana area were found ... | 2010 | 19896221 |
| 9th international feline retrovirus research symposium 2008. preface. | 2010 | 19931187 | |
| coombs', haemoplasma and retrovirus testing in feline anaemia. | to investigate the associations between coombs' testing, haemoplasma and retroviral infections, and feline anaemia. | 2010 | 20070499 |
| characterization of neutrophil extracellular traps in cats naturally infected with feline leukemia virus. | feline leukemia virus (felv), a common, naturally occurring gammaretrovirus in domestic cats, is associated with degenerative diseases of the haematopoietic system, immunodeficiency and neoplasia. felv infection causes an important suppression of neutrophil function, leading to opportunistic infections. recently, a new microbicidal mechanism named netosis was described in human, bovine and fish neutrophils, as well as in chicken heterophils. the purpose of the present study was to characterize n ... | 2010 | 19793908 |
| differential diagnosis of feline leukemia virus subgroups using pseudotype viruses expressing green fluorescent protein. | feline leukemia virus (felv) is classified into three receptor interference subgroups, a, b and c. in this study, to differentiate felv subgroups, we developed a simple assay system using pseudotype viruses expressing green fluorescent protein (gfp). we prepared gfp pseudotype viruses, named gfp(felv-a), gfp(felv-b) and gfp(felv-c) harboring envelopes of felv-a, b and c, respectively. the gfp pseudotype viruses completely interfered with the same subgroups of felv reference strains on fea cells ... | 2010 | 20124765 |
| high prevalence of non-productive felv infection in necropsied cats and significant association with pathological findings. | applying a combination of semi-nested pcr and immunohistology (ihc), the presence of exogenous feline leukemia virus infection was studied in 302 necropsied cats with various disorders. 9% showed the classical outcome of persistent productive felv infection which was represented by felv antigen expression in different organs. 152 cats (50%) harboured exogenous felv-specific proviral sequences in the bone marrow but did not express viral antigen. these cats were considered as horizontally but non ... | 2010 | 20398945 |
| [receptors for animal retroviruses]. | diseases caused by animal retroviruses have been recognized since 19th century in veterinary field. most livestock and companion animals have own retroviruses. to disclose the receptors for these retroviruses will be useful for understanding retroviral pathogenesis, developments of anti-retroviral drugs and vectors for human and animal gene therapies. of retroviruses in veterinary field, receptors for the following viruses have been identified; equine infectious anemia virus, feline immunodefici ... | 2009 | 20218331 |
| single-dose pharmacokinetics and genotoxicity of metronidazole in cats. | single-dose pharmacokinetics and genotoxicity of metronidazole in cats were evaluated. cats received either 5mg/kg metronidazole intravenously, or 20mg/kg metronidazole benzoate (12.4mg/kg metronidazole base) orally in a single dose. serial plasma samples were collected and assayed for metronidazole using high pressure liquid chromatography (hplc). genotoxicity was assessed in vitro in feline peripheral blood mononuclear cells (pbmc) and a feline t-cell lymphoma line incubated with metronidazole ... | 2009 | 19155181 |
| disease threats to the endangered iberian lynx (lynx pardinus). | the iberian lynx, (lynx pardinus), is the most endangered felid in the world. to determine whether sympatric carnivores are reservoirs of pathogens posing a disease risk for the lynx, evidence of exposure to 17 viral, bacterial and protozoan agents was investigated in 176 carnivores comprising 26 free-living lynx, 53 domestic cats, 28 dogs, 33 red foxes (vulpes vulpes), 24 egyptian mongooses (herpestes ichneumon), 10 common genets (genetta genetta) and 2 eurasian badgers (meles meles) in the are ... | 2009 | 18555712 |
| feline leukemia virus and other pathogens as important threats to the survival of the critically endangered iberian lynx (lynx pardinus). | background: the iberian lynx (lynx pardinus) is considered the most endangered felid species in the world. in order to save this species, the spanish authorities implemented a captive breeding program recruiting lynxes from the wild. in this context, a retrospective survey on prevalence of selected feline pathogens in free-ranging lynxes was initiated. methodology/ principal findings: we systematically analyzed the prevalence and importance of seven viral, one protozoan (cytauxzoon felis), and s ... | 2009 | 19270739 |
| myelodysplastic syndromes and acute myeloid leukemia in cats infected with feline leukemia virus clone33 containing a unique long terminal repeat. | feline leukemia virus (felv) clone33 was obtained from a domestic cat with acute myeloid leukemia (aml). the long terminal repeat (ltr) of this virus, like the ltrs present in felv from other cats with aml, differs from the ltrs of other known felv in that it has 3 tandem direct 47-bp repeats in the upstream region of the enhancer (ure). here, we injected cats with felv clone33 and found 41% developed myelodysplastic syndromes (mds) characterized by peripheral blood cytopenias and dysplastic cha ... | 2009 | 19035458 |
| seroprevalence of viral infections in domestic cats in costa rica. | a cross-sectional survey of a convenient sample of domestic cats from costa rica's greater metropolitan area was carried out to determine the prevalence of antibodies against feline herpesvirus type 1 (fhv-1), feline parvovirus (fpv), feline immunodeficiency virus (fiv) and antigens of feline leukemia virus (felv). blood samples were collected from at least 96 cats from june 1998 to december 2001; data related to the individual cats and household variables were obtained using a questionnaire. an ... | 2009 | 19498296 |
| seroprevalence of toxoplasma gondii and concurrent bartonella spp., feline immunodeficiency virus, and feline leukemia virus infections in cats from grenada, west indies. | toxoplasma gondii and bartonella spp. are zoonotic pathogens of cats. feline immunodeficiency virus (fiv) and feline leukemia virus (felv) are related to human immunodeficiency virus, and human leukemia virus, respectively; all of these viruses are immunosuppressive. in the present study, the prevalence of antibodies to t. gondi, bartonella spp., fiv, as well as felv antigen were determined in sera from 75 domestic and 101 feral cats (felis catus) from the caribbean island of grenada, west indie ... | 2009 | 19385716 |
| retroviral infections of small animals. | retroviral infections are particularly important in cats, which are commonly infected with feline leukemia virus and feline immunodeficiency virus. this article describes the biology of these viruses and explores current issues regarding vaccination and diagnosis. the seeming lack of a recognized retrovirus infection in dogs is speculated on, and current and potential future therapies are discussed. | 2008 | 18501285 |
| prevalence and risk factors for hemoplasmas in domestic cats naturally infected with feline immunodeficiency virus and/or feline leukemia virus in rio de janeiro--brazil. | the aim of this study was to determine the prevalence and risk factors for mycoplasma haemofelis (mhf) and 'candidatus mycoplasma haemominutum' (mhm) infections in domestic cats tested for feline immunodeficiency virus (fiv) and feline leukemia virus (felv) with a commercial enzyme-linked immunosorbent assay (elisa) kit. based on serological testing, cats were grouped as i) fiv-positive (n=25); ii) felv-positive (n=39); iii) fiv/felv-positive (n=8); and iv) fiv/felv-negative (n=77). complete blo ... | 2008 | 17905624 |
| association between endogenous feline leukemia virus loads and exogenous feline leukemia virus infection in domestic cats. | recently, we demonstrated that endogenous feline leukemia virus (enfelv) loads may vary among cats of different populations and that felv-infected cats have higher enfelv loads than uninfected cats. thus, we hypothesized that enfelv might influence the pathogenesis and outcome of felv infection. no significant difference in the infection outcome (regressive versus progressive infection) was observed between groups of cats with high or low enfelv loads following felv-a challenge. however, cats wi ... | 2008 | 18407367 |
| genetic characterization of feline leukemia virus from florida panthers. | from 2002 through 2005, an outbreak of feline leukemia virus (felv) occurred in florida panthers (puma concolor coryi). clinical signs included lymphadenopathy, anemia, septicemia, and weight loss; 5 panthers died. not associated with felv outcome were the genetic heritage of the panthers (pure florida vs. texas/florida crosses) and co-infection with feline immunodeficiency virus. genetic analysis of panther felv, designated felv-pco, determined that the outbreak likely came from 1 cross-species ... | 2008 | 18258118 |
| serological survey of toxoplasma gondii infection in domestic cats from northeastern portugal. | cats are very important hosts in the epidemiological cycle of toxoplasma gondii, a zoonotic protozoan parasite that can infect humans and many other animal species worldwide. we report a serological survey of antibodies to t. gondii in domestic cats from northeastern portugal, by means of the modified agglutination test. three cats had titres of 20 (3.9%), 18 had titres of 40 (23.7%) and 55 animals had titres of > or =800 (72.4%). results of three seropositive kittens with less than 4 months wer ... | 2008 | 18571327 |
| naturally occurring feline leukemia virus subgroup a and b infections in urban domestic cats. | a nested-pcr (n-pcr) was used to detect feline leukemia virus (felv) proviral dna in blood samples from 464 sick and 608 healthy domestic cats (felis catus) selected by convenience, and a significantly high prevalence of felv infection was observed. n-pcr results revealed the presence of felv proviral dna in 47.2 % of sick cats and 47.4 % of healthy cats. phylogenetic analysis revealed that felv samples from healthy or sick cats were grouped into separate clades. we determined felv subgroups by ... | 2008 | 18931078 |
| absolute quantitation of feline leukemia virus proviral dna and viral rna loads by taqman real-time pcr and rt-pcr. | sensitive taqman real-time polymerase chain reaction (pcr)-based methods have been developed recently for the detection and quantitation of feline leukemia virus (felv) proviral dna in infected cats. in this chapter, we outline the design and implementation of a taqman real-time pcr assay to quantify total felv proviral and viral rna loads in infected cats. the assay is designed to amplify all three felv subtypes (a-c), but not felv-related endogenous retroviral sequences. the system is tested a ... | 2008 | 18695960 |
| erythrophagocytic low-grade extranodal t-cell lymphoma in a cat. | a 13-year-old male castrated domestic shorthair cat was presented to the referring veterinarian with a 2-month history of weight loss and lethargy. splenomegaly, hepatomegaly, nonregenerative anemia, neutropenia, and hyperbilirubinemia were noted. results of testing for feline immunodeficiency virus, feline leukemia virus, toxoplasma gondii, and mycoplasma sp. were negative. on cytologic examination of aspirates from the enlarged spleen and liver, a population of erythrophagocytic round cells wa ... | 2008 | 19055577 |
| quantification of endogenous and exogenous feline leukemia virus sequences by real-time pcr assays. | endogenous retroviruses are integrated in the genome of most vertebrates. they represent footprints of ancient retroviral infection and are vertically transmitted from parents to their offspring. in the genome of all domestic cats, sequences closely related to exogenous felv known as endogenous feline leukemia virus (enfelv), are present. enfelv are incapable of giving rise to infectious virus particles. however, transcription and translation of enfelv have been demonstrated in tissues of health ... | 2008 | 18295344 |
| functions, structure, and read-through alternative splicing of feline apobec3 genes. | over the past years a variety of host restriction genes have been identified in human and mammals that modulate retrovirus infectivity, replication, assembly, and/or cross-species transmission. among these host-encoded restriction factors, the apobec3 (a3; apolipoprotein b mrna-editing catalytic polypeptide 3) proteins are potent inhibitors of retroviruses and retrotransposons. while primates encode seven of these genes (a3a to a3h), rodents carry only a single a3 gene. | 2008 | 18315870 |
| evaluation of the association of bartonella species, feline herpesvirus 1, feline calicivirus, feline leukemia virus and feline immunodeficiency virus with chronic feline gingivostomatitis. | gingivostomatitis (gs) is a significant condition in cats because of oral discomfort and associated periodontal disease. several infectious agents have been associated with the presence of gs, but a causal relationship is unclear. the cats in this study were housed together, had a history of flea exposure, and were vaccinated with a modified live fvrcp product. there were nine cats with active gs and 36 unaffected cats at the time of sample collection. serum was tested for feline leukemia virus ... | 2008 | 17766156 |
| epizootiology and management of feline leukemia virus in the florida puma. | feline leukemia virus (felv) was not detected in florida pumas (puma concolor coryi) in almost 20 yr of surveillance; however, the finding of two felv antigen-positive pumas during the 2002-2003 capture season led to an investigation of felv in the population. between january 1990 and april 2007, the proportion of pumas testing felv antibody positive increased, with antibody-positive pumas concentrated in the northern portion of puma range. five of 131 (4%) pumas sampled between july 2000 and ap ... | 2008 | 18689639 |
| how molecular methods change our views of felv infection and vaccination. | felv was discovered 40 years ago and vaccines have been commercially available for almost two decades. so far, most felv pathogenesis and vaccine studies were conducted assaying parameters, such as virus isolation and antigen detection. accordingly, regressive infection was characterized by transient or undetectable viremia, while persistent viremia is typically observed in cats with progressive infection. using real-time polymerase chain reaction assays, the spectrum of host response categories ... | 2008 | 18295346 |
| acute monoblastic leukemia in a felv-positive cat. | a 1.6-year-old male domestic short hair cat was brought to the veterinary medical teaching hospital, kasetsart university, with signs of severe anemia, depression, and general lymph node enlargement. complete blood count revealed leukocytosis and massive undifferentiated blasts. testing for antibodies specific to feline leukemia virus (felv) was positive, and felv nucleic acid was confirmed by nested polymerase chain reaction. base on cytochemistry and ultrastructure, the cat was diagnosed with ... | 2008 | 18296895 |
| development and application of a quantitative real-time pcr assay to detect feline leukemia virus rna. | we previously defined four categories of feline leukemia virus (felv) infection, designated as abortive, regressive, latent, and progressive. to determine if detectable viral dna is transcriptionally active in the absence of antigenemia, we developed and validated a real-time viral rna qpcr assay. this assay proved to be highly sensitive, specific, reproducible, and allowed reliable quantitation. we then applied this methodology, together with real-time dna qpcr and p27 capsid antigen capture el ... | 2008 | 18321595 |
| copy number polymorphism of endogenous feline leukemia virus-like sequences. | in the cat genome, endogenous feline leukemia virus (enfelv) exists as multiple, nearly full-length proviral sequences. even though no infectious virus is produced from enfelv sequences, transcription and translation have been demonstrated in tissues of healthy cats and in feline cell lines. to test the hypothesis that the enfelv loads play a role in exogenous felv-a infection and pathogenesis, we designed three real-time pcr assays to quantify u3 and env enfelv loads (two within u3 amplifying d ... | 2007 | 17329079 |
| worldwide occurrence of feline hemoplasma infections in wild felid species. | while hemoplasma infections in domestic cats are well studied, almost no information is available on their occurrence in wild felids. the aims of the present study were to investigate wild felid species as possible reservoirs of feline hemoplasmas and the molecular characterization of the hemoplasma isolates. blood samples from the following 257 wild felids were analyzed: 35 iberian lynxes from spain, 36 eurasian lynxes from switzerland, 31 european wildcats from france, 45 lions from tanzania, ... | 2007 | 17301277 |
| evidence of feline immunodeficiency virus, feline leukemia virus, and toxoplasma gondii in feral cats on mauna kea, hawaii. | we determined prevalence to feline immunodeficiency virus (fiv) antibodies, feline leukemia virus (felv) antigen, and toxoplasma gondii antibodies in feral cats (felis catus) on mauna kea hawaii from april 2002 to may 2004. six of 68 (8.8%) and 11 of 68 (16.2%) cats were antibody positive to fiv and antigen positive for felv, respectively; 25 of 67 (37.3%) cats were seropositive to t. gondii. antibodies to felv and t. gondii occurred in all age and sex classes, but fiv occurred only in adult mal ... | 2007 | 17495320 |
| diminished potential for b-lymphoid differentiation after murine leukemia virus infection in vivo and in eml hematopoietic progenitor cells. | infection with a recombinant murine-feline gammaretrovirus, mofe2, or with the parent virus, moloney murine leukemia virus, caused significant reduction in b-lymphoid differentiation of bone marrow at 2 to 8 weeks postinfection. the suppression was selective, in that myeloid potential was significantly increased by infection. analysis of cell surface markers and immunoglobulin h gene rearrangements in an in vitro model demonstrated normal b-lymphoid differentiation after infection but significan ... | 2007 | 17428873 |
| defibrinated bovine plasma inhibits retroviral transcription by blocking p52 activation of the nfkappab element in the long terminal repeat. | bovine leukemia virus (blv) induces a persistent but latent infection in cattle. viral latency is invoked by a protein known as plasma blocking factor (pbf) that is found in both bovine and human plasma. we report here on pathways that mediate latency in the presence of pbf. reporter-gene constructs driven by the promoters of 6 retroviruses were used to measure the production of chloramphenicol acetyl transferase (cat) in cell lines cultured with or without defibrinated bovine plasma. plasma inh ... | 2007 | 17479775 |
| plasma electrophoretogram in feline immunodeficiency virus (fiv) and/or feline leukaemia virus (felv) infections. | the electrophoretogram of 89 cats, including those infected by feline immunodeficiency virus (fiv+), feline leukaemia virus (felv+) and non-infected, showed statistically significant differences in several of the fractions. fiv+ cats had very high protein values (mean, 8.10 g/dl), mostly because of hypergammaglobulinemia (mean, 2.81 g/dl) as compared with non-infected animals and felv+. in addition, in these fiv+ animals, the albumin/globulins ratio (a/g) was very low (mean, 0.72). statistically ... | 2007 | 17493167 |
| quality of different in-clinic test systems for feline immunodeficiency virus and feline leukaemia virus infection. | many new diagnostic in-house tests for identification of feline immunodeficiency virus (fiv) and feline leukaemia virus (felv) infection have been licensed for use in veterinary practice, and the question of the relative merits of these kits has prompted comparative studies. this study was designed to define the strengths and weaknesses of seven fiv and eight felv tests that are commercially available. in this study, 536 serum samples from randomly selected cats were tested. those samples reacti ... | 2007 | 17604205 |
| association of germ-line polymorphisms in the feline p53 gene with genetic predisposition to vaccine-associated feline sarcoma. | a case-control study was conducted in order to investigate the association of polymorphisms in the genomic sequence of the feline p53 gene with a predisposition to vaccine-associated feline sarcoma (vafs). in the study, 50 domestic short hair cats with a confirmed histopathologic diagnosis of vafs were matched to disease-free controls (1:2) by age, sex, and breed. cats from both the diseased (case) and control groups were also negative for feline leukemia virus and feline immunodeficiency virus. ... | 2007 | 17641147 |
| follow-up of the health conditions of an urban colony of free-roaming cats (felis catus linnaeus, 1758) in the city of rio de janeiro, brazil. | similar to other urban areas where food and shelter are abundant, the zoological garden of rio de janeiro has dealt for years with a colony of feral or semi-feral domestic cats. a survey was conducted during 2002-2004 as a follow-up to a previous study in 2001 of the cat colony to identify pathogens circulating among the population and to annually follow the status of the cats to analyze morbidity coefficients and associations among infections and infestations identified in the colony. during th ... | 2007 | 17481822 |
| mediastinal lymphoma in a young turkish angora cat. | an 8-month old intact male turkish angora cat was referred to the veterinary medical teaching hospital (vmth), seoul national university, for an evaluation of anorexia and severe dyspnea. the thoracic radiographs revealed significant pleural effusion. a cytology evaluation of the pleural fluid strongly suggested a lymphoma containing variable sized lymphocytes with frequent mitotic figures and prominent nucleoli. the feline leukemia virus and feline immunodeficiency virus tests were negative. th ... | 2006 | 16645348 |
| rapid detection of feline leukemia virus provirus integration into feline genomic dna. | feline leukemia virus (felv) is a gamma retrovirus that induces fatal diseases in domestic cats. efficacious felv vaccines prevent persistent viremia and development of felv-related disease after virus exposure, but not minimal viral replication and a provirus-positive state as recently demonstrated using sensitive real-time pcr assays. proviral integration is an important parameter of latent infection and persistence of retroviruses in infected cells. so far, felv-specific real-time pcr assays ... | 2006 | 16488115 |