Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| vaccination against feline leukaemia using a new feline herpesvirus type 1 vector. | a recombinant feline herpesvirus type 1 (fhv-1) was constructed expressing the envelope glycoprotein gene from feline leukaemia virus (felv). the expression cassette containing the long terminal repeat promoter from rous sarcoma virus was stably integrated at the locus downstream of the gc homologue in fhv-1. oronasal vaccination with recombinant fhv-1 engendered significant protection against challenge with the homologous felv-a/glasgow-1 isolate. three of four vaccinated cats did not become vi ... | 1996 | 9014292 |
| feline leukemia virus detection by elisa and pcr in peripheral blood from 68 cats with high, moderate, or low suspicion of having felv-related disease. | clinicopathologic criteria were used to group 68 cats according to high, moderate, or low suspicion of having feline leukemia virus (felv)-related disease. peripheral blood samples were tested for felv antigen by enzyme-linked immunosorbent assay (elisa) and for felv dna by polymerase chain reaction (pcr). there was no significant difference between elisa and pcr results in the 68 cats. in the high-suspicion group, 46%(11/24) of cytopenic cats were test positive (elisa and pcr) and 87% (13/15) w ... | 1996 | 9026077 |
| feline immunodeficiency virus (fiv) infection in cats at necropsy: a serological study. | sera collected post mortem during a 6-month period from cats were tested for feline immunodeficiency virus (fiv)-specific antibodies by (1) an enzyme-linked immunosorbent assay (elisa), (2) an indirect peroxidase-based immunocytological test (ip), (3) a western immunoblotting (wb) method with fiv-infected cell lysates, and (4) a wb method with purified viral antigen. all four methods were capable of detecting fiv-specific antibodies in haemolysed sera. however, the elisa showed the lowest "posit ... | 1997 | 9179747 |
| cooperating events in lymphomagenesis mediated by feline leukemia virus. | feline leukemia virus (felv)-mediated lymphomagenesis in the domestic cat has been examined as a model of lymphoid malignancy in a naturally outbreeding population. the pathogenesis of two distinct, naturally occurring types of felv-induced tumors has been investigated: (1) a thymic lymphoma of t-cell origin, typical of felv-induced lymphoma, and (2) an extrathymic, extranodal lymphoma of non-b non-t-cell origin. the genetic features of these tumors are clearly distinguishable, and include deter ... | 1997 | 9209353 |
| dynamics of two feline retroviruses (fiv and felv) within one population of cats. | we present a deterministic model of the dynamics of two microparasites simultaneously infecting a single host population. both microparasites are feline retroviruses, namely feline immunodeficiency virus (fiv) and feline leukaemia virus (felv). the host is the domestic cat felis catus. the model has been tested with data generated by a long-term study of several natural cat populations. stability analysis and simulations show that, once introduced in a population, fiv spreads and is maintained, ... | 1997 | 9225475 |
| molecular characteristics of malignant lymphomas in cats naturally infected with feline immunodeficiency virus. | neoplastic disease, especially malignant lymphomas, are often observed in cats infected with feline immunodeficiency virus (fiv). in order to clarify the characteristics of lymphoma cells and to investigate the pathogenesis in fiv-infected cats, we examined the lymphoma tissues developed in five cats naturally infected with fiv by southern blot analyses using feline immunoglobulin (ig), t-cell receptors (tcr) and fiv probes. all of the five cases were serologically positive for anti-fiv antibody ... | 1997 | 9261955 |
| [placebo-controlled double-blind study on the efficacy of a paramunity inducer in the treatment of naturally felv-infected cats]. | the study involved 120 cats, of which 60 were treated with baypamun hk and 60 cats received a preparation of virus free cell culture medium. therapeutic efficacy was determined by monitoring the general status of health, body weight, skin, lymph nodes, oral cavity, the presence of the p27 antigen and the felv p27 antigen concentration in serum. no statistically significant differences between both groups could be demonstrated neither for clinical nor for virologic parameters. remission of the vi ... | 1997 | 9289888 |
| modelling the feline leukemia virus (felv) in natural populations of cats (felis catus). | a compartmental model was built in order to study the circulation and impact of feline leukemia virus (felv) in populations of domestic cats. the model was tested with data from a long-term study of several feline populations. the study of stability shows that felv is maintained in the population with a stable equilibrium and a slight reduction of population size. estimation of the transmission rate allows us to make a comparison with the values previously estimated in the literature. we compare ... | 1997 | 9345792 |
| the feline leukemia virus long terminal repeat contains a potent genetic determinant of t-cell lymphomagenicity. | feline leukemia virus (felv) is an important pathogen of domestic cats. the most common type of malignancy associated with felv is t-cell lymphoma. sl3-3 (sl3) is a potent t-cell lymphomagenic murine leukemia virus. transcriptional enhancer sequences within the long terminal repeats (ltrs) of sl3 and other murine retroviruses are crucial genetic determinants of the pathogenicities of these viruses. the ltr enhancer sequences of felv contain identical binding sites for some of the transcription f ... | 1997 | 9371646 |
| structure and function of the long terminal repeats of feline leukemia viruses derived from naturally occurring acute myeloid leukemias in cats. | long terminal repeats of feline leukemia viruses cloned from feline acute myeloid leukemias frequently contained direct repeats of 40 to 74 bp in the upstream region of the enhancer (ure). the repetitive ure conferred an enhancer function upon gene expression in myeloid cells, suggesting its association with tumorigenic potential in myeloid cells. | 1997 | 9371654 |
| a comparison of antibody responses to veterinary vaccine antigens potentiated by different adjuvants. | six adjuvant formulations were compared for their ability to potentiate the primary and memory antibody responses in mice to three companion animal vaccine immunogens--feline leukemia virus (felv), feline immunodeficiency virus (fiv), and a recombinantly-derived heartworm antigen. the combination of a novel bacterial immunostimulator, gliding bacterial adjuvant (gba), either adsorbed onto an aluminum hydroxide gel (rehydragel hpa), or emulsified with a vehicle of polyalcohol and detergent, elici ... | 1997 | 9413100 |
| parameters of disease progression in long-term experimental feline retrovirus (feline immunodeficiency virus and feline leukemia virus) infections: hematology, clinical chemistry, and lymphocyte subsets. | after several years of latency, feline immunodeficiency virus (fiv) and feline leukemia virus (felv) cause fatal disease in the cat. the aim of this study was to determine laboratory parameters characteristic of disease progression which would allow a better description of the asymptomatic phase and a better understanding of the pathogenesis of the two infections. therefore, experimentally infected cats (fiv and/or felv positive) and control animals were observed over a period of 6.5 years under ... | 1997 | 9008278 |
| tumorigenic potential of a recombinant retrovirus containing sequences from moloney murine leukemia virus and feline leukemia virus. | a recombinant retrovirus, termed mofe2-mulv, was constructed in which the u3 region of t-lymphomagenic moloney murine leukemia virus (mo-mulv) was replaced by that of felv-945, a provirus of unique long terminal repeat (ltr) structure identified only in non-t-cell, non-b-cell lymphomas of the domestic cat. the ltr of felv-945 is unusual in that it contains only a single copy of the transcriptional enhancer followed 25 bp downstream by a 21-bp sequence in triplicate in tandem. infectivity of mofe ... | 1998 | 9445002 |
| retroviruses and sexual size dimorphism in domestic cats (felis catus l.). | hochberg and co-workers have predicted that an increase in host adult mortality due to parasites is balanced by an earlier age at first reproduction. in polygynous species we hypothesize that such a pattern would lead to diverging selection pressure on body size between sexes and increased sexual size dimorphism. in polygynous mammals, male body size is considered to be an important factor for reproductive success. thus, under the pressure of a virulent infection, males should be selected for ra ... | 1998 | 9493404 |
| the host range and interference properties of two closely related feline leukemia variants suggest that they use distinct receptors. | the proviral clones 61e and 61c represent two closely related variants of feline leukemia virus (felv) that exhibit significant differences in their biological and pathogenic properties. the major pathogenic determinant has been mapped to the extracellular envelope glycoprotein (env-su), but the mechanism by which envelope differences influence pathogenesis is not well understood. moreover, it is unclear whether these viruses infect the same target cells and/or enter cells using the same recepto ... | 1998 | 9514965 |
| the receptor binding site of feline leukemia virus surface glycoprotein is distinct from the site involved in virus neutralization. | the external surface glycoprotein (su) of feline leukemia virus (felv) contains sites which define the viral subgroup and induce virus-neutralizing antibodies. the subgroup phenotypic determinants have been located to a small variable region, vr1, towards the amino terminus of su. the sites which function as neutralizing epitopes in vivo are unknown. recombinant su proteins were produced by using baculoviruses that contained sequences encoding the sus of felv subgroup a (felv-a), felv-c, and two ... | 1998 | 9525654 |
| clinicopathological and immunological characteristics of six cats with granular lymphocyte tumors. | clinical and immunological characteristics were investigated in six cases of feline granular lymphocyte (gl) tumor. the ages of the affected cats were relatively old, ranging from 4 to 13 years of age. gastrointestinal signs were commonly observed in these cases. only one of the six gl tumor cases was positive for feline leukemia virus (felv) antigen. phenotypic analysis revealed that the gl tumor cells from all of the six cases lacked the t- or b-cell markers. these gl tumor cells were examined ... | 1998 | 9597305 |
| dynamics of a feline retrovirus (felv) in host populations with variable spatial structure. | the predictions of epidemic models are remarkably affected by the underlying assumptions concerning host population dynamics and the relation between host density and disease transmission. furthermore, hypotheses underlying distinct models are rarely tested. domestic cats (felis catus) can be used to compare models and test their predictions, because cat populations show variable spatial structure that probably results in variability in the relation between density and disease transmission. cat ... | 1998 | 9684375 |
| feline lymphoma (145 cases): proliferation indices, cluster of differentiation 3 immunoreactivity, and their association with prognosis in 90 cats. | paraffin-embedded, formalin-fixed tissue samples from 145 cats with lymphoma were analyzed for cluster of differentiation 3 (cd3, a surface antigen) immunoreactivity, argyrophilic nucleolar organizer region (agnor) frequency, and proliferating cell nuclear antigen labeling index (pcna-li). this information along with signalment, anatomic site, and feline leukemia virus (felv) antigen status was used to determine the potential of these indicators to predict response to therapy, remission, and sur ... | 1998 | 9773411 |
| hepatozoon species infection in domestic cats: a retrospective study. | hepatozoon sp. is a protozoan parasite of peripheral blood neutrophils in cats. feline hepatozoonosis has been reported infrequently and little is known about the pathogenesis of this infection. in order to further clarify clinicopathological characteristics of hepatozoonosis in domestic cats, a retrospecitve study of hepatozoonosis in cats admitted during 1989-1995 to the hebrew university school of veterinary medicine was conducted. the study population comprised all the cats whose medical rec ... | 1998 | 9806492 |
| genetic analysis of the rat leukemia virus: influence of viral sequences in transduction of the c-ras proto-oncogene and expression of its transforming activity. | the rat leukemia virus (ralv) is an endogenous retrovirus that is spontaneously released by sprague-dawley rat embryo cells. the overall structure of the ralv genome resembles that of other simple, replication-competent retroviruses, but the sequence of the long terminal repeats (ltr) is unique and unrelated to the known retroviruses. phylogenetically, the ralv genome appears to be more closely related to the feline leukemia virus group of retroviruses than to the murine leukemia virus group. a ... | 1998 | 9811727 |
| primary central nervous system t-cell lymphoma in a cat. | a 5-year-old intact male persian cat was presented with progressive neurologic deficits, hyperesthesia, hyperreflexia, ataxia, and intention tremors, which resulted in death. serologic tests for feline leukemia virus and feline immunodeficiency virus infection were negative. neurohistologic examination revealed the presence of pleomorphic cellular infiltrates in cerebral leptomeninges and around parenchymal vessels. the majority of infiltrating cells were uniformly immunostained using an antiser ... | 1998 | 9823600 |
| feline immunodeficiency virus (fiv) infection leads to increased incidence of feline odontoclastic resorptive lesions (forl). | feline odontoclastic resorptive lesions (forl), previously known as 'neck lesions,' are commonly known in domestic, but also in non-domestic cats. they are characterized by odontoclastic resorptive processes, which take place at the dental root and at the periodontium. chronic inflammation of gingiva and periodontium is believed to be an important etiological factor in the development of forl. in this context, various feline viruses have been discussed to play a relevant role in the pathogenesis ... | 1998 | 9839881 |
| prevalence of igg and igm antibodies specific to toxoplasma gondii in cats. | igg and igm antibodies specific to toxoplasma gondii were determined simultaneously by ifat in cats. group 1 included 357 cats with various diagnoses, but negative for felv and fiv. specific igg antibodies in titres ranging from 10 to 2560 (mean 247) were detected in 61.3% animals, specific igm antibodies were found only in one cat (0.28%) in titre 1:40 showing an igg titre of 160. oocyst shedding was demonstrated in one cat (0.28%) showing an igg titre of 1:40. group 2 included 33 cats positive ... | 1998 | 9870370 |
| retrovirus receptor pit-1 of the felis catus. | we isolated a cdna encoding a feline homolog of human pit-1, a sodium-dependent phosphate symporter which is utilized by gibbon ape leukemia virus (galv) as a receptor for entry into host cells. the overall homology between the human and feline receptors is 92 and 93% at the nucleotide and deduced amino acid levels, respectively. hydropathy analyses implied ten potential membrane spanning regions and, in analogy to human and murine homologs, five extracellular and four intracellular loops. strik ... | 1998 | 9878855 |
| feline viruses in wildcats from scotland. | few data are available on the prevalence of feline viruses in european wildcats (felis silvestris). previous surveys have indicated that wildcats may be infected with the common viruses of domestic cats, apart from feline immunodeficiency virus (fiv). in the present study, 50 wildcats trapped throughout scotland (uk) between august 1992 and january 1997 were tested for evidence of viral infection. all were negative for fiv by several serological or virological methods. by contrast, 10% of the ca ... | 1999 | 10073361 |
| feline leukemia virus long terminal repeat activates collagenase iv gene expression through ap-1. | leukemia and lymphoma induced by feline leukemia viruses (felvs) are the commonest forms of illness in domestic cats. these viruses do not contain oncogenes, and the source of their pathogenic activity is not clearly understood. mechanisms involving proto-oncogene activation subsequent to proviral integration and/or development of recombinant viruses with enhanced replication properties are thought to play an important role in their disease pathogenesis. in addition, the long terminal repeat (lt ... | 1999 | 10233955 |
| the fit-1 common integration locus in human and mouse is closely linked to myb. | the fit-1 locus was originally identified as a common insertion site for feline leukemia virus (felv) in thymic lymphosarcomas induced by felv-myc recombinant viruses, suggesting that it harbors a gene that cooperates with myc in t-cell leukemogenesis. we have previously mapped the fit-1 locus to feline chromosome (chr) b2. we have now identified conserved sequences that allow the mapping of the murine homolog using the european interspecific backcross (eucib). this shows that fit-1 is located o ... | 1999 | 10341084 |
| a putative cell surface receptor for anemia-inducing feline leukemia virus subgroup c is a member of a transporter superfamily. | domestic cats infected with the horizontally transmitted feline leukemia virus subgroup a (felv-a) often produce mutants (termed felv-c) that bind to a distinct cell surface receptor and cause severe aplastic anemia in vivo and erythroblast destruction in bone marrow cultures. the major determinant for felv-c-induced anemia has been mapped to a small region of the surface envelope glycoprotein that is responsible for its receptor binding specificity. thus, erythroblast destruction may directly o ... | 1999 | 10400745 |
| treatment of feline leukemia virus (felv) infection. | felv infection is still considered to account for most disease-related deaths in pet cats. different treatment attempts with various drugs were performed in the past but none resulted in healing or complete virus elimination. therefore, it caused a sensation when horber and mayr [horber, d., mayr, b., 1991. prax. 19, 311-314; horber, d., schnabl, w., mayr, b., 1992. tierarztl. umschau 47, 556-560; mayr, b., horber, d., 1992. kleintierprax. 37, 515-518] published that they were able to cure 80 to ... | 1999 | 10515279 |
| high prevalence of borna disease virus in domestic cats with neurological disorders in japan. | a total of 15 (t-1-t-15) domestic cats with neurological disorders in tokyo area were examined for association with borna disease virus (bdv). none had detectable antibodies to feline immunodeficiency virus (fiv), feline leukemia virus, feline infectious peritonitis virus and toxoplasma gondii, and only cat t-8 had detectable antibody to fiv. serological and molecular epidemiological studies revealed a significantly high prevalence of bdv infection in these cats: antibodies against bdv p24 and/o ... | 1999 | 10596800 |
| the effects of feline retroviruses on cytokine expression. | to identify potential roles of cytokines in retroviral pathogenesis, we used reverse transcription-quantitative competitive polymerase chain reaction (rt-qcpcr) assays to characterize mrna levels of 19 different lymphokines, chemokines, monokines and hematopoietic growth factors in three feline cell lines productively infected with subgroup a feline leukemia virus (felv-a) or various feline immunodeficiency virus (fiv) strains. infection of a cd8+, cd5- large granular lymphocyte (lgl) cell line ... | 1999 | 10628677 |
| use of nested polymerase chain reaction (pcr) for detection of retroviruses from formalin-fixed, paraffin-embedded uveal melanomas in cats. | thirty-six formalin-fixed, paraffin-embedded enucleated globes from cats with a diagnosis of diffuse anterior uveal melanoma were obtained. sections of tumor were excised, deparaffinized, and subjected to nested polymerase chain reaction (pcr) to identify proviral dna sequences from the feline leukemia virus (felv)-feline sarcoma virus (fesv; 36 eyes), and the feline immunodeficiency virus (fiv; 18 eyes). all samples tested were negative for fiv dna. three samples were positive for felv-fesv dna ... | 1999 | 11397251 |
| prevalence of fiv and felv infections in cats in istanbul. | 2000 | 11716594 | |
| a 13-amino-acid pit1-specific loop 4 sequence confers feline leukemia virus subgroup b receptor function upon pit2. | feline leukemia virus subgroup b (felv-b) and gibbon ape leukemia virus (galv) utilize the human protein pit1 but not the related protein, pit2, as receptor. a stretch of 9 amino acids, named region a, was identified in the putative fourth extracellular loop of pit1 (residues 550 through 558) as critical for felv-b and galv receptor function. however, the presence of pit1 region a did not confer receptor function for felv-b upon pit2, while it did so for galv. we have here shown that the presenc ... | 2000 | 10684313 |
| prevalence of enteric zoonotic organisms in cats. | to determine prevalence of enteric zoonotic organisms in cats in north-central colorado. | 2000 | 10707683 |
| chronic myelomonocytic leukemia in a cat. | a 1-year-old spayed domestic short-haired cat was referred with anorexia and weight loss. hematologic findings indicated nonregenerative anemia, severe neutropenia and monocytosis. the feline leukemia virus (felv) antigen test was positive reaction by enzyme-linked immunosorbent assay. dysgranulopoiesis with slight increase in blast cells were observed in bone marrow smears. on the basis of blood and bone marrow findings, the cat was diagnosed as chronic myelomonocytic leukemia (cmmol), which po ... | 2000 | 10720191 |
| prevalence and pathogenicity of retroviruses in wildcats in france. | feline leukaemia virus (felv) and feline immunodeficiency virus (fiv) are frequently encountered in domestic cats (felis catus) and in wild felids, but only felv has been previously identified in wildcats (fellis silvestris). thirty-eight wildcats, either captured alive or found dead, were sampled in eastern and central france. nine of them (23.7 per cent) carried the felv p27 antigen, and three (7.9 per cent) had antibodies to fiv. there was a significant relationship between two measures of bo ... | 2000 | 10766116 |
| safety and efficacy of a recombinant felv vaccine combined with a live feline rhinotracheitis, calicivirus and panleukopenia vaccine. | 2000 | 10803986 | |
| weakly coupled hyperbolic systems modeling the circulation of felv in structured feline populations. | global existence and regularity results are provided for weakly coupled first order hyperbolic systems modeling the propagation of the feline leukemia virus (felv), a retrovirus of domestic cats (felis catus). in a simple example we find a threshold parameter yielding endemic stationary states. | 2000 | 10804261 |
| long-term impact on a closed household of pet cats of natural infection with feline coronavirus, feline leukaemia virus and feline immunodeficiency virus. | a closed household of 26 cats in which feline coronavirus (fcov), feline leukaemia virus (felv) and feline immunodeficiency virus (fiv) were endemic was observed for 10 years. each cat was seropositive for fcov on at least one occasion and the infection was maintained by reinfection. after 10 years, three of six surviving cats were still seropositive. only one cat, which was also infected with fiv, developed feline infectious peritonitis (fip). rising anti-fcov antibody titres did not indicate t ... | 2000 | 10811262 |
| differential pathogenicity of two feline leukemia virus subgroup a molecular clones, pfra and pf6a. | f6a, a molecular clone of subgroup a feline leukemia virus (felv) is considered to be highly infectious but weakly pathogenic. in recent studies with a closely related subgroup a molecular clone, fra, we demonstrated high pathogenicity and a strong propensity to undergo recombination with endogenous felv (enfelv), leading to a high frequency of transition from subgroup a to a/b. the present study was undertaken to identify mechanisms of felv pathogenesis that might become evident by comparing th ... | 2000 | 10846058 |
| precise gene localization by phenotypic assay of radiation hybrid cells. | a high resolution map of the human genome previously has been constructed by using the g3 panel of human/hamster radiation hybrid cell lines and >15,000 unique human genetic markers. by determining whether human dna sequences are present or absent in each of the hybrids, localization of single genes may routinely be achieved at approximately 250-kb resolution. in this paper we have tested whether similarly precise localization might be achieved by phenotypic screening of the hybrids to facilitat ... | 2000 | 10852967 |
| transduction of feline hematopoietic cells by oncoretroviral vectors pseudotyped with the subgroup a feline leukemia virus (felv-a). | the domestic cat is an outbred species with many identified analogues of human genetic diseases. therefore, it has the potential to serve as a large animal model for evaluating the feasibility of hematopoietic stem cell gene therapy. this study compared gene transfer rates into feline hematopoietic progenitors by oncoretroviral vectors pseudotyped with the subgroup a feline leukemia virus (felv-a), the gibbon ape leukemia virus (galv), and the murine amphotropic virus. gene transfer rates were s ... | 2000 | 10899828 |
| contrastive prevalence of feline retrovirus infections between northern and southern vietnam. | the prevalence of infections with three feline retroviruses; feline leukemia virus (felv), feline immunodeficiency virus (fiv) and feline foamy virus (fefv), was examined in domestic cats (felis catus) and leopard cats (felis bengalensis) in southern vietnam in 1998. we then compared this data with our previous study in northern vietnam in 1997. none of the cats had felv antigens in both the northern and southern areas. in contrast, there is a great distinction in the seropositivity of fiv. twen ... | 2000 | 10993195 |
| feline leukemia virus and feline immunodeficiency virus. | ophthalmic manifestations of felv or fiv infection can occur in all ocular tissues and may be manifestations of direct viral effects or secondary to viral-related malignant transformation. additionally, the manifestations of common feline ophthalmic pathogens may be more severe and poorly responsive to therapy because of the immunosuppressive effects of felv or fiv infection. prompt diagnosis of underlying viral infection in cats with ophthalmic disease is paramount for accurate diagnosis and pr ... | 2000 | 11033869 |
| clonality analysis of various hematopoietic disorders in cats naturally infected with feline leukemia virus. | the clonality analysis of the bone marrow cells was carried out by detecting the integrated proviruses of feline leukemia virus (felv) to understand the pathogenesis of felv-associated hematopoietic disorders in cats. bone marrow cells from 4 cases with acute myeloid leukemia (aml), 9 cases with myelodysplastic syndromes (mds), 2 cases with pure red cell aplasia (prca) and 3 healthy carriers infected with felv were subjected to southern blot analyses using an exogenous felv probe. clonal hematop ... | 2000 | 11073076 |
| evidence that an ires within the notch2 coding region can direct expression of a nuclear form of the protein. | we previously reported the isolation from a thymic tumor of a feline leukemia virus that had transduced a fragment of the notch2 gene. here we present evidence that a nuclear form of notch2 corresponding to the biologically active intracellular domain (n2icd) is expressed from this recombinant retrovirus through internal ribosome entry. internal ribosome entry sites (iress) are rna structural motifs that allow 5' cap-independent recruitment of ribosomal subunits to mrnas. the notch2 ires maps ex ... | 2000 | 11090631 |
| vaccine-associated sarcomas in cats: a unique cancer model. | epidemiologic evidence supports a relationship between vaccination of cats for rabies and feline leukemia virus with the development of soft tissue sarcomas at the site of administration. these tumors are locally invasive and histologically aggressive. as with high-grade soft tissue sarcoma in humans, combination treatment with radiation therapy and surgery provides for optimum tumor control. feline vaccine-associated sarcoma has become a difficult issue for the veterinary profession for legal, ... | 2001 | 11153990 |
| feline leukemia virus in a captive bobcat. | an 11-mo-old captive-bred male neutered bobcat (felis rufus) presented with lethargy, anorexia, leukopenia, neutropenia, lymphopenia, and nonregenerative anemia. the animal was diagnosed as feline leukemia virus (felv) positive by immunofluorescent antibody and enzyme-linked immunosorbant assay (elisa) testing. it died despite supportive care. pathologic examination revealed multifocal non-suppurative encephalitis, diffuse interstitial pneumonia, multifocal hepatocellular necrosis, non-suppurati ... | 2001 | 11272497 |
| feline vaccine-associated fibrosarcoma: an ultrastructural study of 20 tumors (1996-1999). | twenty feline vaccine-associated sarcomas were examined by transmission electron microscopy. tumors contained pleomorphic spindle cells, histiocytoid cells, and giant cells. most tumors contained myofibroblasts, which had morphologic features similar to those of fibroblasts. these cells were further distinguished by subplasmalemmal dense plaques and thin cytoplasmic actin myofilaments organized as elongated bundles concentrated at irregular intervals forming characteristic dense bodies. intracel ... | 2001 | 11280376 |
| feline leukaemia virus neutralising antibodies. | 2001 | 11761301 | |
| comparison of endogenous feline leukemia virus rna content in feline vaccine and nonvaccine site-associated sarcomas. | to determine whether feline vaccine site-associated sarcomas (vss) contain a higher amount of endogenous felv (enfelv) rna, compared with feline nonvaccine site-associated sarcomas (non-vss). | 2001 | 11763193 |
| identification of envelope determinants of feline leukemia virus subgroup b that permit infection and gene transfer to cells expressing human pit1 or pit2. | the retroviral vector systems that are in common use for gene therapy are designed to infect cells expressing either of two widely expressed phosphate transporter proteins, pit1 or pit2. subgroup b feline leukemia viruses (felv-bs) use the gibbon ape leukemia virus receptor, pit1, as a receptor for entry. our previous studies showed that some chimeric envelope proteins encoding portions of felv-b could also enter cells by using a related receptor protein, pit2, which serves as the amphotropic mu ... | 2001 | 11435563 |
| combined omental pedicle grafts and thoracodorsal axial pattern flaps for the reconstruction of chronic, nonhealing axillary wounds in cats. | to assess the results of an omental pedicle graft in combination with a thoracodorsal axial pattern flap for the reconstruction of chronic nonhealing axillary wounds in 10 cats caused by forelimb entrapment within a collar. | 2001 | 11443600 |
| comparative examination of cats with feline leukemia virus-associated enteritis and other relevant forms of feline enteritis. | cats with feline leukemia virus (felv)-associated enteritis (fae), enteritis of other known viral etiology (parvovirus [pv], enteric coronavirus [cov]), and enteritis of unknown etiology with histologic features similar to those of fae and pv enteritis (eue) and felv-negative and felv-positive cats without enterocyte alterations were examined. amount and types of infiltrating leukocytes in the jejunum and activity and cellular constituents of mesenteric lymph nodes, spleen, and bone marrow were ... | 2001 | 11467470 |
| bone marrow colony-forming unit assay in cats with naturally occurring myelodysplastic syndromes. | feline myelodysplastic syndromes (mds) has been diagnosed in many cats infected with feline leukemia virus, although the pathogenesis of this hematopoietic deficiency has been unclear. in this study, we assayed the bone marrow erythroid colony-forming units (cfu-e) and granulocyte-machrophage cfus (cfu-gm) to investigate the pathogenesis of feline mds. the number of cfu-e colonies was decreased in 4 of 7 cats with mds, and the number of cfu-gm colonies was also decreased in 4 cats. furthermore, ... | 2001 | 11503958 |
| a replication-competent feline leukemia virus, subgroup a (felv-a), tagged with green fluorescent protein reporter exhibits in vitro biological properties similar to those of the parental felv-a. | we previously established that lymphoid tumors could be induced in cats by intradermal injection of ecotropic feline leukemia virus (felv), subgroup a, plasmid dna. in preparation for in vivo experiments to study the cell-to-cell pathway for the spread of the virus from the site of inoculation, the green fluorescent protein (gfp) transgene fused to an internal ribosome entry site (ires) was inserted after the last nucleotide of the env gene in the ecotropic felv-a rickard (fra) provirus. the eng ... | 2001 | 11507228 |
| therapy for australian cats with lymphosarcoma. | to determine the response of australian cats with lymphosarcoma to chemotherapy and/or surgery in relation to patient and tumour characteristics, haematological and serum biochemical values and retroviral status. | 2001 | 11837901 |
| protection against oronasal challenge with virulent feline leukaemia virus lasts for at least 12 months following a primary course of immunisation with leukocell 2 vaccine. | the duration of immunity provided by a feline leukemia virus (felv) vaccine, leukocell 2, was determined. kittens were vaccinated when 9 and 12 weeks of age and were challenged 12 months later with felv-a/glasgow-1. an oronasal challenge protocol without corticosteroid enhancement was developed in order to induce a persistent viraemia in a high proportion of adult cats. fourteen of 18 (80%) of the vaccinated cats challenged in this way remained non-viraemic while 9/15 (60%) of age-matched contro ... | 2002 | 12126896 |
| retrospective study of 46 cases of feline haemobartonellosis in israel and their relationships with felv and fiv infections. | forty-six cats with clinical haemobartonellosis were studied; 75 per cent of the cats of known age were two-and-a-half years old or younger, 50 per cent were intact males and 19.5 per cent were castrated males. the predominant signs of the disease were tachypnoea, lethargy, depression, anorexia, infestation with fleas, pale mucous membranes, icterus, emaciation, dehydration, splenomegaly, anaemia, leucocytosis, increased activities of alanine aminotransferase and aspartate aminotransferase, and ... | 2002 | 12164225 |
| prevalence of feline leukemia virus infection and serum antibodies against feline immunodeficiency virus in unowned free-roaming cats. | to determine prevalence of felv infection and serum antibodies against feline immunodeficiency virus (fiv) in unowned free-roaming cats. | 2002 | 12418520 |
| deluge of questions prompts aafp to develop fiv vaccine brief. american association of feline practitioners. | 2002 | 12418682 | |
| analysis of the disease potential of a recombinant retrovirus containing friend murine leukemia virus sequences and a unique long terminal repeat from feline leukemia virus. | we have molecularly cloned a feline leukemia virus (felv) (clone 33) from a domestic cat with acute myeloid leukemia (aml). the long terminal repeat (ltr) of this virus, like the ltrs present in felv proviruses from other cats with aml, contains an unusual structure in its u3 region upstream of the enhancer (ure) consisting of three tandem direct repeats of 47 bp. to test the disease potential and specificity of this unique felv ltr, we replaced the u3 region of the ltr of the erythroleukemia-in ... | 2002 | 11773427 |
| longitudinal analysis of feline leukemia virus-specific cytotoxic t lymphocytes: correlation with recovery from infection. | feline leukemia virus (felv) is a common naturally occurring gammaretrovirus of domestic cats that is associated with degenerative diseases of the hematopoietic system, immunodeficiency, and neoplasia. although the majority of cats exposed to felv develop a transient infection and recover, a proportion of cats become persistently viremic and many subsequently develop fatal diseases. to define the dominant host immune effector mechanisms responsible for the outcome of infection, we studied the lo ... | 2002 | 11836409 |
| a highly efficient gene delivery system derived from feline immunodeficiency virus (fiv). | 2003 | 12526177 | |
| manufacturer addresses concerns about fiv vaccine. | 2003 | 12555972 | |
| demineralization for inactivation of infectious retrovirus in systemically infected cortical bone: in vitro and in vivo experimental studies. | clinical and experimental studies have demonstrated viral transmission through the transplantation of fresh-frozen infected bone. while sterilization methods sufficient to inactivate the human immunodeficiency virus (hiv) have been shown to markedly alter osteoconductive and osteoinductive properties of bone allografts, the ability of a process for creating demineralized bone matrix to abrogate transmission of a retrovirus has not been investigated, to our knowledge. we hypothesized that a clini ... | 2003 | 12571312 |
| 2001 report of the american association of feline practitioners and academy of feline medicine advisory panel on feline retrovirus testing and management. | 2003 | 12613492 | |
| feline leukaemia virus and vaccination. | 2003 | 12670435 | |
| a potential therapeutic strategy to combat leukemia virus infection. | to test the concept that a replication-competent retrovirus carrying a suicide gene could have potential utility in the control of the natural virus infection in mammalian species, we constructed derivatives of a feline leukemia virus (felv) that is commonly associated with leukemia-lymphomas in this species. the felv, rickard strain, subgroup a (fra) genome contained at the 3' end of the envgene, an insert of an internal ribosomal entry site (ires) linked to cdna sequence of either herpes simpl ... | 2003 | 12673127 |
| seroprevalence of bartonella henselae, toxoplasma gondii, fiv and felv infections in domestic cats in japan. | seroprevalence of bartonella henselae, toxoplasma gondii, feline immunodeficiency virus (fiv) and feline leukemia virus (felv) infections was investigated in 1,447 domestic cats derived from the north (hokkaido) to the south (okinawa) prefectures in japan. of the cats investigated, 8.8% (128/1,447) were seropositive to b. henselae, 5.4% (78/1,447) to t. gondii, 9.8% (107/1,088) to fiv, and 2.9% (32/1,088) to felv, respectively. for b. henselae infection, the positive rate varied from 11.5% in ca ... | 2003 | 12680718 |
| serosurvey for feline leukemia virus and lentiviruses in captive small neotropic felids in são paulo state, brazil. | feline leukemia virus (felv), gammaretrovirus, and feline immunodeficiency virus, a lentivirus, are members of the family retroviridae, and may establish persistent infections in the domestic cat (felis catus). cytoproliferative and cytosuppressive disorders may result from infection with these viruses. morbidity and mortality rates are high in domestic cats worldwide. infection of endangered neotropic small felids with these viruses could be devastating. to investigate the prevalence of felv an ... | 2003 | 12723802 |
| principles of treatment for feline lymphoma. | lymphoma is the most commonly diagnosed neoplasm in cats. as feline leukemia virus antigenemia has decreased over the past 15 years, there has been a profound shift in the presence, signalment, and frequency of sites of feline lymphoma in north america. there is variation in anatomic classification systems, but most studies have divided lymphoma into four groups: alimentary, mediastinal, multicentric, or extranodal. clinical signs and common differential diagnoses for each of the forms are descr ... | 2003 | 12831069 |
| disease propagation in connected host populations with density-dependent dynamics: the case of the feline leukemia virus. | spatial heterogeneity is a strong determinant of host-parasite relationships, however local-scale mechanisms are often not elucidated. generally speaking, in many circumstances dispersal is expected to increase disease persistence. we consider the case when host populations show density-dependent dynamics and are connected through the dispersal of individuals. taking the domestic cats (felis catus)--feline leukemia virus (felv) as a toy model of host-microparasite system, we predict the disease ... | 2003 | 12875824 |
| evidence of horizontal transmission of feline leukemia virus by the cat flea ( ctenocephalides felis). | the feline leukemia virus (felv) is a naturally occurring and widespread retrovirus among domestic cats. the virus is mainly transmitted horizontally through saliva, blood and other body fluids by close contact between cats. vectors other than cats, e.g. blood-sucking parasites, have not been reported. this study tested the vector potential of the cat flea ( ctenocephalides felis) for felv. in a first feeding, fleas were fed for 24 h with blood from a felv-infected cat with persistent viremia. f ... | 2003 | 14557874 |
| multicentric t-cell lymphoma associated with feline leukemia virus infection in a captive namibian cheetah (acinonyx jubatus). | this case report describes a multicentric lymphoma in a 4 yr old female wildborn captive cheetah (acinonyx jubatus) in namibia after being housed in an enclosure adjacent to a feline leukemia virus (felv) infected cheetah that had previously been in contact with domestic cats. the year prior to the onset of clinical signs, the wild-born cheetah was felv antigen negative. the cheetah subsequently developed lymphoma, was found to be infected with felv, and then rapidly deteriorated and died. at ne ... | 2003 | 14567232 |
| t-cell lymphoma of the tympanic bulla in a feline leukemia virus-negative cat. | this report constitutes the first description of a t-cell lymphoma of the tympanic bulla in a cat. this feline leukemia virus (felv)-negative cat originally presented with signs referable to middle ear disease; it deteriorated rapidly after definitive diagnosis. lymphoma of the middle ear is extremely rare in all species. | 2003 | 14703086 |
| prevalence and risk factors for heartworm infection in cats from northern florida. | necropsies were performed on 630 adult cats in northern florida to determine the prevalence and risk factors for heartworm infection in cats of this region. heartworms were identified in 4.9% of cats, and serological evidence of heartworm exposure was present in 17% of cats. not all cats from which heartworms were recovered were seropositive for heartworm antigen or antibody. there was no association between heartworm infection and co-infection with feline leukemia virus (felv) or feline immunod ... | 2003 | 14736717 |
| exposure to feline and canine pathogens in bobcats and gray foxes in urban and rural zones of a national park in california. | exposure of bobcats (lynx rufus) and gray foxes (urocyon cinereoargenteus) to a range of common canine and feline pathogens was assessed in urban and rural zones of golden gate national recreation area, a national park in the san francisco bay area, (california, usa) from 1992 to 1995. testing included serology for canine distemper virus, canine parvovirus (cpv), canine adenovirus, leptospira interrogans, feline calicivirus (fcv), feline panleukopenia virus, feline herpesvirus, feline enteric co ... | 2004 | 15137484 |
| serosurvey of viral infections in free-ranging namibian cheetahs (acinonyx jubatus). | cheetahs (acinonyx jubatus) in captivity have unusually high morbidity and mortality from infectious diseases, a trait that could be an outcome of population homogeneity or the immunomodulating effects of chronic stress. free-ranging namibian cheetahs share ancestry with captive cheetahs, but their susceptibility to infectious diseases has not been investigated. the largest remaining population of free-ranging cheetahs resides on namibian farmlands, where they share habitat with domestic dogs an ... | 2004 | 15137485 |
| centrosome amplification and chromosomal instability in feline lymphoma cell lines. | to evaluate the presence of centrosome amplification and the resulting chromosomal instability in cat tumors, a newly established feline lymphoma cell line and four already established feline lymphoma cell lines were examined using immunohistochemical analysis of centrosomes. the number of chromosomes were subsequently counted by metaphase spread. moreover, to explore whether mutational inactivation of the p53 gene or inactivation of the p53 protein caused by mdm2 gene overexpression, occurred i ... | 2004 | 15297751 |
| prevalence of infectious diseases in feral cats in northern florida. | objectives of this study were to determine prevalence of infection in feral cats in northern florida with a select group of infectious organisms and to determine risk factors for infection. blood samples or sera from 553 cats were tested with a panel of antibody, antigen or pcr assays. male cats were at higher risk for fiv, mycoplasma haemofelis, and m. haemominutum. infection with either felv or fiv was associated with increased risk for coinfection with the other retrovirus, m. haemofelis, or ... | 2004 | 15363760 |
| identification of a human heme exporter that is essential for erythropoiesis. | flvcr, a member of the major facilitator superfamily of transporter proteins, is the cell surface receptor for feline leukemia virus, subgroup c. retroviral interference with flvcr display results in a loss of erythroid progenitors (colony-forming units-erythroid, cfu-e) and severe anemia in cats. in this report, we demonstrate that human flvcr exports cytoplasmic heme and hypothesize that human flvcr is required on developing erythroid cells to protect them from heme toxicity. inhibition of flv ... | 2004 | 15369674 |
| regulation of felv-945 by c-myb binding and cbp recruitment to the ltr. | feline leukemia virus (felv) induces degenerative, proliferative and malignant hematologic disorders in its natural host, the domestic cat. felv-945 is a viral variant identified as predominant in a cohort of naturally infected animals. felv-945 contains a unique sequence motif in the long terminal repeat (ltr) comprised of a single copy of transcriptional enhancer followed by a 21-bp sequence triplicated in tandem. the ltr is precisely conserved among independent cases of multicentric lymphoma, ... | 2004 | 15507152 |
| sanitary conditions of a colony of urban feral cats (felis catus linnaeus, 1758) in a zoological garden of rio de janeiro, brazil. | the colony of urban stray cats living in the rio de janeiro zoological garden was studied in order to develop a population and health control program. as many cats as possible were captured during two months (47 animals) and were classified according to gender, age, weight and coat markings. they were submitted to a general health evaluation, examined for the presence of ectoparasites and sent to a surgical neutering program. all animals had a blood sample drawn for cbc, platelet count, heartwor ... | 2004 | 15517031 |
| characterization of a newly established nonproducer lymphoma cell line for feline leukemia virus. | a feline lymphoblastoid cell line (ko-1) was established from a 5-year-old neutered female cat with naturally occurring thymic lymphoma. ko-1 cells had a rearrangement of t-cell receptor beta-chain gene and a germ-line configuration of immunoglobulin heavy chain gene, however, they were devoid of t-cell-specific surface phenotype. cytogenetically, ko-1 cells showed a hyperploidy (2n = 41) due to the trisomy of b2, f2 and x chromosomes. although ko-1 cells were shown to be clonally expanded cells ... | 2004 | 15541796 |
| feline immunodeficiency virus diagnosis after vaccination. | prior to the widespread use of vaccination for the control of feline immunodeficiency virus (fiv) infection, diagnosis was made by the detection of antibodies against fiv. a number of commercial animal side tests perform quite well for this determination, with positive predictive values between 91 and 100% and negative predictive values between 96 and 100%. furthermore, results of these tests could be confirmed by western blot analysis of fiv test-positive sera. currently, a killed whole virus f ... | 2004 | 15984349 |
| in vitro selective suppression of feline myeloid colony formation is attributable to molecularly cloned strain of feline leukemia virus with unique long terminal repeat. | molecularly cloned feline leukemia virus (felv)-clone 33 (c-33), derived from a cat with acute myelocytic leukemia (aml), was examined to assess its relation to the pathogenesis of aml and myelodysplastic syndrome (mds). to evaluate in vitro pathogenicity of felv c-33, bone marrow colony-forming assay was performed on marrow cells infected with felv c-33 or an felv subgroup a strain (61e, a molecularly cloned strain with minimal pathogenicity). the myeloid colony-forming activity of feline bone ... | 2005 | 15563922 |
| substitution of feline leukemia virus long terminal repeat sequences into murine leukemia virus alters the pattern of insertional activation and identifies new common insertion sites. | the recombinant retrovirus, mofe2-mulv (mofe2), was constructed by replacing the u3 region of moloney murine leukemia virus (m-mulv) with homologous sequences from the felv-945 ltr. nih/swiss mice neonatally inoculated with mofe2 developed t-cell lymphomas of immature thymocyte surface phenotype. mofe2 integrated infrequently (0 to 9%) near common insertion sites (ciss) previously identified for either parent virus. using three different strategies, ciss in mofe2-induced tumors were identified a ... | 2005 | 15596801 |
| subtle mutational changes in the su protein of a natural feline leukemia virus subgroup a isolate alter disease spectrum. | felv-945 is a representative isolate of the natural feline leukemia virus (felv) variant predominant in non-t-cell malignant, proliferative, and degenerative diseases in a geographic cohort. the felv-945 surface glycoprotein (su) is closely related to natural horizontally transmissible felv subgroup a (felv-a) but was found to differ from a prototype to a larger extent than the members of felv-a differ among themselves. the sequence differences included point mutations restricted largely to the ... | 2005 | 15650161 |
| insertional polymorphisms of endogenous feline leukemia viruses. | the number, chromosomal distribution, and insertional polymorphisms of endogenous feline leukemia viruses (enfelvs) were determined in four domestic cats (burmese, egyptian mau, persian, and nonbreed) using fluorescent in situ hybridization and radiation hybrid mapping. twenty-nine distinct enfelv loci were detected across 12 of the 18 autosomes. each cat carried enfelv at only 9 to 16 of the loci, and many loci were heterozygous for presence of the provirus. thus, an average of 19 autosomal cop ... | 2005 | 15767400 |
| experimental quantification of the feline leukaemia virus in the cat flea (ctenocephalides felis) and its faeces. | cat fleas (ctenocephalides felis) were fed via artificial membranes and infected with the feline leukaemia virus (felv) from cell cultures. after removing the fleas from the blood source, the quantity of virus in the flea and its faeces was measured over a defined period of time. the virus was detectable in the fleas for up to 30 h at room temperature and up to 115 h at 4 degrees c. in the faeces, the amount of virus decreased much more slowly--after 2 weeks half of the initial amount of virus c ... | 2005 | 16228264 |
| serologic survey of domestic felids in the petén region of guatemala. | blood samples were analyzed from 30 domestic cats (felis domesticus) from the petén region of guatemala to determine the seroprevalence of common pathogens that may pose a potential risk to native wild felids. eight of the cats had been vaccinated previously; however, owners were unable to fully describe the type of vaccine and date of administration. in addition, blood samples were obtained from two captive margays (leopardus wiedii). samples were tested for antibodies to feline immunodeficienc ... | 2005 | 17315469 |
| real-time reverse transcription polymerase chain reaction assay to detect a broad range of feline calicivirus isolates. | this report describes a real-time reverse transcription polymerase chain reaction (rt-pcr) assay with sybr green targeting the vp2 (orf 3) of feline caliciviruses. all of the 44 feline calicivirus isolates tested were detected, whereas neither feline herpesvirus, feline panleukopenia virus, feline immunodeficiency virus and feline leukemia virus nor other calicivirus like rabbit hemorrhagic disease virus and a canine calicivirus isolate showed specific amplification products. the sensitivity of ... | 2006 | 16266755 |
| rapid detection of feline leukemia virus provirus integration into feline genomic dna. | feline leukemia virus (felv) is a gamma retrovirus that induces fatal diseases in domestic cats. efficacious felv vaccines prevent persistent viremia and development of felv-related disease after virus exposure, but not minimal viral replication and a provirus-positive state as recently demonstrated using sensitive real-time pcr assays. proviral integration is an important parameter of latent infection and persistence of retroviruses in infected cells. so far, felv-specific real-time pcr assays ... | 2006 | 16488115 |
| protection from challenge following administration of a canarypox virus-vectored recombinant feline leukemia virus vaccine in cats previously vaccinated with a killed virus vaccine. | to compare protection against felv challenge obtained following administration of 2 doses of an adjuvanted, chemically inactivated, whole felv (felv-k) vaccine with protection obtained following administration of 1 dose of an felv-k vaccine followed by 1 dose of a canarypox virus-vectored recombinant felv (rcp-felv) vaccine. | 2006 | 16506935 |
| feline leukaemia virus (felv) and feline immunodeficiency virus infections in cats in the pisa district of tuscany, and attempts to control felv infection in a colony of domestic cats by vaccination. | the seroprevalence of feline immunodeficiency virus (fiv) in 203 apparently healthy domestic cats living in the district of pisa, central italy, was 11.3 per cent, and the prevalence of feline leukaemia virus (felv) was 8.4 per cent. the prevalence of fiv depended significantly on the lifestyle and age of the cats; cats living outdoors were more likely to be fiv-positive than cats living indoors, and the proportion of fiv-positive cats increased with age. in contrast, there was no significant re ... | 2006 | 16632529 |
| mediastinal lymphoma in a young turkish angora cat. | an 8-month old intact male turkish angora cat was referred to the veterinary medical teaching hospital (vmth), seoul national university, for an evaluation of anorexia and severe dyspnea. the thoracic radiographs revealed significant pleural effusion. a cytology evaluation of the pleural fluid strongly suggested a lymphoma containing variable sized lymphocytes with frequent mitotic figures and prominent nucleoli. the feline leukemia virus and feline immunodeficiency virus tests were negative. th ... | 2006 | 16645348 |
| worldwide occurrence of feline hemoplasma infections in wild felid species. | while hemoplasma infections in domestic cats are well studied, almost no information is available on their occurrence in wild felids. the aims of the present study were to investigate wild felid species as possible reservoirs of feline hemoplasmas and the molecular characterization of the hemoplasma isolates. blood samples from the following 257 wild felids were analyzed: 35 iberian lynxes from spain, 36 eurasian lynxes from switzerland, 31 european wildcats from france, 45 lions from tanzania, ... | 2007 | 17301277 |