| replication of equid herpesvirus 4 in endothelial cells and synovia of a field case of viral pneumonia and synovitis in a foal. | equid herpesvirus 4 (ehv-4) infection was diagnosed as the cause of interstitial pneumonia in a 6-week-old conventionally reared welsh pony foal, by cocultivation and immunolabelling with specific monoclonal antibodies, ehv-4 specific amplification of viral dna, and immunohistological examination of infected tissues. the case was novel in that replication of the ehv-4 isolate in endothelial cells and in the synovial epithelium was a feature. restriction digests of this isolate were compared with ... | 1995 | 7769144 |
| equine herpesviruses 4 (equine rhinopneumonitis virus) and 1 (equine abortion virus). | | 1995 | 7793324 |
| immunologic relationships between equine herpesvirus type 1 (equine abortion virus) and type 4 (equine rhinopneumonitis virus). | the specificity of selected immune responses to equine herpesvirus type 1 (ehv-1) and type 4 (ehv-4) was examined in 3 colostrum-deprived specific-pathogen-free foals. single foals were vaccinated with inactivated ehv-1, inactivated ehv-4, or control cell lysate plus adjuvant followed by successive intranasal challenge exposures with ehv-1 and ehv-4 or with ehv-4 and ehv-1. vaccination with inactivated virus preparations elicited cellular immune responses and antibody which were augmented by sub ... | 1984 | 6208822 |
| vaccination of pregnant ponies against equine rhinopneumonitis. | bovine herpesvirus 1247 (one dose) was given subcutaneously to five pregnant pony mares between 227 and 319 days of their gestations. there were no adverse clinical reactions, and the virus was not recovered from nasal swabs collected during a 2-week period after vaccination. four ponies foaled full-term, live, healthy foals. the foal of the fifth mare (no. 1) was found dead, but on the basis of the pathologic and virologic examinations, the virus was not considered to be the cause of the death. ... | 1980 | 6254412 |
| another fence jumped in the ehv-1 stakes. | | 1994 | 7889915 |
| distribution of equid herpesvirus-1 (ehv-1) in the respiratory tract of ponies: implications for vaccination strategies. | twelve adult ponies and 2 conventional foals were exposed to 10(6.6) tcid50 of equid herpesvirus-1 (ehv-1), strain ab4 and samples of respiratory tract tissues were recovered. infectious virus in tissue homogenates was detected using susceptible cell monolayers and expression of viral antigens was monitored using indirect immunoperoxidase histochemistry of paraffin sections. the results illustrated the rapid dissemination of ehv-1 throughout the respiratory tract, with early replication in the l ... | 1994 | 7889920 |
| infection of the central nervous system of horses with equine herpesvirus serotype 1. | during the last 2 years different equine herpesviruses serotype 1 strains have been isolated from cases of paretic or paralytic disease among horses in the federal republic of germany. in this paper the available information is collated and briefly reviewed. a short description of the symptoms and the possible mechanism of the pathogenesis are given. | 1981 | 6273556 |
| mapping, cloning and sequencing of a glycoprotein-encoding gene from bovine herpesvirus type 1 homologous to the ge gene from hsv-1. | in order to map and identify the glycoprotein-encoding gene from bovine herpesvirus type 1 (bhv-1), homologous to the ge glycoprotein from herpes simplex virus type 1 (hsv-1), a region of the unique short sequence from the bhv-1 genome has been sequenced. the sequenced region contains an orf coding for a polypeptide of 575 amino acids (aa). the aa sequence presents substantial similarity to that of the glycoprotein ge from hsv-1 and to homologous proteins of related viruses such as pseudorabies ... | 1994 | 7958994 |
| nucleocapsid mass and capsomer protein stoichiometry in equine herpesvirus 1: scanning transmission electron microscopic study. | the brookhaven scanning transmission electron microscope was employed to measure the masses of two nucleocapsid species (of light and intermediate densities) of equine herpesvirus 1. these were found to be 196.7 +/- 9.2 and 229.0 +/- 9.5 megadaltons (mda), respectively. biochemical assays showed that neither nucleocapsid contained any significant amount of dna (less than 0.2% [wt/wt]). taking into account data on protein composition, we conclude that the difference between their masses is essent ... | 1989 | 2760983 |
| the extreme carboxyl terminus of the equine herpesvirus 1 homolog of herpes simplex virus vp16 is essential for immediate-early gene activation. | gene 12 of equine herpesvirus 1 (ehv-1), the homolog of herpes simplex virus (hsv) vp16 (alpha tif, vmw65), was cloned into a eukaryotic expression vector by pcr and used in transactivation studies of both the ehv-1 and hsv-1 ie1 promoters. results demonstrated that the product of gene 12 is a potent transactivator of immediate-early gene expression of both viruses, which requires sequences in the upstream hsv-1 promoter for activity. mutational analysis of the gene 12 open reading frame indicat ... | 1994 | 8035487 |
| serological and virological investigations of an equid herpesvirus 1 (ehv-1) abortion storm on a stud farm in 1985. | an extensive outbreak of ehv-1 abortions occurred on a stud farm in england in 1985. of the 67 pregnant mares present on the stud farm, 31 were challenged with ehv-1, resulting in the loss of 22 fetuses or foals. laboratory investigations revealed that the spread of the virus closely followed movement of apparently healthy mares (during the incubation period of the infection). during the outbreak mares were challenged 1-4 months before the expected foaling date. there was no relationship between ... | 1987 | 2824770 |
| large and small subunits of the aujeszky's disease virus ribonucleotide reductase: nucleotide sequence and putative structure. | we determined the entire dna sequence of two adjacent open reading frames of aujeszky's disease virus encoding ribonucleotide reductase genes with the intergenic sequence of 9 bp. from the sequence analysis we deduce that orfs encode large and small subunits, with sizes of 835 and 303 amino acids, respectively. amino acid sequence comparison of adv rr2 with that of equine herpesvirus type 1, bovine herpesvirus type 1, hsv-1 and varicella zoster virus revealed that 48% of amino acids represent cl ... | 1994 | 8086454 |
| molecular evolution of herpesviruses: genomic and protein sequence comparisons. | phylogenetic reconstruction of herpesvirus evolution is generally founded on amino acid sequence comparisons of specific proteins. these are relevant to the evolution of the specific gene (or set of genes), but the resulting phylogeny may vary depending on the particular sequence chosen for analysis (or comparison). in the first part of this report, we compare 13 herpesvirus genomes by using a new multidimensional methodology based on distance measures and partial orderings of dinucleotide relat ... | 1994 | 8107249 |
| the equine herpesvirus type 1 glycoprotein homologous to herpes simplex virus type 1 glycoprotein m is a major constituent of the virus particle. | glycoprotein 45 is a major envelope glycoprotein of equine herpesvirus type 1. the gene encoding this protein is located between map units 0.615 and 0.636 on the virus genome and evidence has suggested that it is encoded by gene 52, one of four genes within this region. using pcr we have amplified gene 52 and subsequently cloned it into a mammalian expression vector under the control of the human cytomegalovirus immediate early gene promoter. the gene was expressed in cos-7 cells and its product ... | 1994 | 8113768 |
| characterization of two equine herpesvirus (ehv) isolates associated with neurological disorders in horses. | | 1987 | 2829471 |
| demonstration of equine herpes virus 1 (ehv-1) in histological sections and tissue cultures by the peroxidase-antiperoxidase (pap) technique. | | 1987 | 2830744 |
| [recent information about the etiopathogenesis of paretic-paralytic forms of herpesvirus infection in horses]. | from spring 1990 to summer 1991 we investigated 21 horses with clinical symptoms of ehv-infection by means of serological and virological methods including dna-hybridization to identify the causative agents. the results indicated that, as already reported by us, ehv4 may also cause the paralytic form of the infection. the possibility of double infection with ehv4 and ehv1 cannot be excluded. in 3 out of 21 affected horses we could investigate brain tissue and/or spinal fluid by dotblot hybridiza ... | 1993 | 8248905 |
| neuropathogenicity for suckling mice of equine herpesvirus 1 from the lipizzan outbreak 1983 and of selected other ehv 1 strains. | | 1987 | 2830747 |
| association of microbiologic flora with clinical, endoscopic, and pulmonary cytologic findings in foals with distal respiratory tract infection. | undifferentiated distal respiratory tract disease (nasal discharge, cough, pneumonia) in foals (1 to 8 months old) is a burdensome economic problem on breeding farms; yet, the infective agents associated with these episodes have not been well described. possible causes of these episodes of illness were investigated by culturing specimens of proximal and distal airways of clinically diseased foals (n = 101), prior to any treatment, for aerobic and anaerobic bacteria and viruses (rhinoviruses, equ ... | 1993 | 8250386 |
| [new observations in the diagnosis of ehv (equine herpes virus) abortions]. | over the last years the percentage of foals lost by ehv at term or close to term seems to be higher than in former years. furthermore, the pathological findings seem to shift from liver to lung. so far there has been no explanation for this phenomenon. | 1987 | 2831640 |
| a suspected case of equine herpesvirus 1 encephalomyelitis. | | 1987 | 2833882 |
| isolation and characterization of monoclonal antibodies against an attenuated vaccine strain of equine herpesvirus type 1 (ehv-1). | the production and differentiation of monoclonal antibodies (mabs) against the rac-h strain of ehv-1 used as an attenuated live vaccine to prevent rhinopneumonitis and abortion is described. seven different antigenic sites were detected by the 15 mabs produced. ehv-1 specific mabs as well as ehv-1 and -4 common mabs could be established, allowing easy typing of ehv isolates. one mab recognized the vaccine strain only. this reaction was used to investigate a possible involvement of the vaccine st ... | 1988 | 2847404 |
| response of ponies to adjuvanted ehv-1 whole virus vaccine and challenge with virus of the homologous strain. | five yearling ponies were vaccinated with inactivated equid herpesvirus type 1 (ehv-1) in freund's complete adjuvant as a double emulsion and revaccinated 6 weeks later with ehv-1 in freund's incomplete adjuvant. these ponies and three age-matched controls were challenged intra-nasally after a further 6 weeks with homologous live virus and monitored clinically, biologically and serologically. after challenge, clinical signs were mild in both groups. no cell-associated viraemias were detected in ... | 1995 | 7735868 |
| the horserace betting levy board's code of practice for equine viral arteritis for the 1994 breeding season. | the horserace betting levy board formulates codes of practice for the control of contagious equine metritis and other equine bacterial venereal diseases, and equine viral arteritis and equid herpesvirus 1. this year's codes have just been published and the code of practice for eva, reproduced below, has been substantially amended following the recent outbreak in the uk. the code is intended for use by veterinary surgeons and breeders of thoroughbred and non-thoroughbred horses. the hblb states t ... | 1993 | 8310626 |
| an immunohistological study of the uterus of mares following experimental infection by equid herpesvirus 1. | twelve welsh mountain pony mares in late gestation were infected intranasally with ehv-1 (ab4 isolate) at dose rates from 10(3) to 10(7.3) tcid50. this resulted in 3 cases of paresis, at days 9, 10 and 12 after inoculation, and 5 abortions, at days 6, 9, 18, 19 and 20. euthanasia was performed between days 6 and 21, with collection of uterine specimens for histopathology, virus isolation and immunoperoxidase staining from the pregnant horn, non-pregnant horn and body. ehv-1 replication in endome ... | 1993 | 8380768 |
| the pseudorabies virus host-shutoff homolog gene: nucleotide sequence and comparison with alphaherpesvirus protein counterparts. | the virion host shutoff function (vhs) of many herpesviruses is required for inhibition of cellular gene expression in infected cells. this function corresponds to the ul41 open reading frame of herpes simplex virus type 1 (hsv-1) and homolog sequences have been found in other alphaherpesvirus, like hsv-2, varicella-zoster virus (vzv) and equine herpesvirus type 1 (ehv-1). in this work, we have cloned and sequenced a pseudorabies virus (prv) gene which is homologous to the vhs genes of the other ... | 1993 | 8384744 |
| nuclear localization and transcriptional activation activities of truncated versions of the immediate-early gene product of equine herpesvirus 1. | the equine herpesvirus 1 (ehv-1) immediate-early (ie) gene product encodes a nuclear regulatory protein capable of negatively autoregulating its own promoter, transactivating representative ehv-1 early promoters, and acting in a concerted fashion with accessory ehv-1 regulatory factors to transactivate ehv-1 late promoters. to identify ie amino acid sequences involved in nuclear localization and to examine the contribution of c-terminal portions of the ie polypeptide to transactivation, vectors ... | 1995 | 7745735 |
| expression of membrane-bound and secreted forms of equine herpesvirus 1 glycoprotein d by recombinant baculovirus. | analyses of the synthesis and processing of recombinant full-length glycoprotein d of equine herpesvirus type 1 (ehv-1; gd392) or recombinant truncated gd (gd352) expressed in baculovirus-infected sf9 cells revealed the following: (1) gd polypeptides encoded by both recombinant baculoviruses react with gd-specific antibodies including peptide-specific antiserum that neutralizes ehv-1 in a plaque reduction assay, (2) both the full-length recombinant gd392 and the truncated gd352 are expressed pre ... | 1995 | 7754672 |
| development of a dna probe for identification of bovine herpesvirus 4. | a sensitive and specific dna probe for detection and identification of bovine herpesvirus 4 (bhv-4) was developed. cloned fragments from a library of hindiii fragments of the bhv-4 (dn-599) genome were labeled with 32p or digoxigenin and were tested for sensitivity and specificity in detecting viral dna by dot-blot hybridization. two probes were identified that detected 10 pg of purified viral dna, and detected viral dna in 0.001 micrograms of total dna extracted from bhv-4-infected cells. both ... | 1993 | 8391228 |
| identification and nucleotide sequence of a gene in feline herpesvirus type 1 homologous to the herpes simplex virus gene encoding the glycoprotein h. | a gene encoding the glycoprotein h (gh) homologue of feline herpesvirus type 1 was identified and sequenced. it was located immediately downstream of the thymidine kinase gene within an ecori 6.6 kbp fragment. in addition, a partial ul21 homologous gene was located downstream of the gh homologous gene. the primary translation product of the gh homologous gene is predicted to consist of 821 amino acids with a molecular weight of 92.5 kda. it possesses several characteristics typical of transmembr ... | 1993 | 8394688 |
| evidence for a relationship between equine abortion (herpes) virus deoxyribonucleic acid synthesis and the s phase of the kb cell mitotic cycle. | autoradiographic analyses of deoxyribonucleic acid (dna) synthesis in randomly growing kb cell cultures infected with equine abortion virus (eav) suggested that viral dna synthesis was initiated only at times that coincided with the entry of noninfected control cells into the s phase of the cell cycle. synchronized cultures of kb cells were infected at different stages of the cell cycle, and rates of synthesis of cellular and viral dna were measured. when cells were infected at different times w ... | 1971 | 4254680 |
| immunization with glycoprotein c of equine herpesvirus-1 is associated with accelerated virus clearance in a murine model. | the glycoprotein c (gc) gene of equine herpesvirus-1 (ehv-1) was expressed in insect cells by a recombinant baculovirus as several products with apparent molecular weights of 66 kda-80 kda. the baculovirus ehv-1 gc products were recognised by monoclonal antibody and by ehv-1 convalescent equine sera, indicating conservation of antigenic determinants and confirming this glycoprotein as a target for the equine immune system. mice immunized with recombinant ehv-1 gc showed accelerated clearance of ... | 1995 | 7794119 |
| the equine herpesvirus 1 ir6 protein is nonessential for virus growth in vitro and modified by serial virus passage in cell culture. | the ir6 protein of different plaque isolates from three passages of the equine herpesvirus 1 strain rac was investigated. southern blot and dna sequence analyses revealed that plaque isolates from the 12th passage (racl11 and racl22) retained both copies of the ir6 gene, whereas two different genotypes were observed by the 185th passage: racm24 still harbored both copies of the ir6 gene, whereas racm36 deleted one of the two copies. in the 256th passage (rach), both copies of the ir6 gene were a ... | 1996 | 8610435 |
| localization of the us protein kinase of equine herpesvirus type 1 is affected by the cytoplasmic structures formed by the noval ir6 protein. | previous work revealed that the us (unique short) segment of equine herpesvirus type-1 (ehv-1), like that of other alphaherpesviruses, encodes a serine/threonine protein kinase (pk). experiments were carried out to identify the pk encoded by the ehv-1 eus2 gene (orf 69) and to ascertain its time course of synthesis and cellular localization. western blot and immunoprecipitation analyses of ehv-1-infected cell extracts using a pk-specific polyclonal antibody generated against a bacterially expres ... | 1996 | 8661393 |
| prolonged gene expression and cell survival after infection by a herpes simplex virus mutant defective in the immediate-early genes encoding icp4, icp27, and icp22. | very early in infection, herpes simplex virus (hsv) expresses four immediate-early (ie) regulatory proteins, icp4, icp0, icp22, and icp27. the systematic inactivation of sets of the ie proteins in cis, and the subsequent phenotypic analysis of the resulting mutants, should provide insights into how these proteins function in the hsv life cycle and also into the specific macromolecular events that are altered or perturbed in cells infected with virus strains blocked very early in infection. this ... | 1996 | 8709264 |
| amplification of strains of bovine herpesvirus 1 by use of polymerase chain reaction with primers in the thymidine kinase region. | a primer pair was designed from the published nucleotide sequence of the coding region of the bovine herpesvirus 1 (bhv-1) thymidine kinase (tk) gene for use in detection of the virus by use of polymerase chain reaction (pcr) amplification of 12 bhv-1 strains (3 atcc and 9 local isolates). a tk deletion mutant bhv-1, and 2 bhv-4 strains from atcc were used as negative controls. one strain each of feline herpes-virus, equine herpesvirus, and bovine adenovirus, and 2 noninoculated bovine cultured ... | 1994 | 7802385 |
| expression and characterisation of equine herpesvirus 1 glycoprotein h using a recombinant baculovirus. | a recombinant baculovirus capable of expressing the glycoprotein h (gh) gene of equine herpesvirus 1 (ehv-1) was constructed. ehv-1 gh gene products in recombinant baculovirus infected insect cells were identified as 105 kda and 110 kda species compared with a 115 kda product detected in ehv-1 infected mammalian cells. the extent of n-glycosylation of ehv-1 gh in both insect and mammalian cells was indicated by a shift in apparent molecular weights after pngase f treatment to 90 kda and 95 kda f ... | 1994 | 7944958 |
| alterations in the equine herpesvirus type-1 (ehv-1) strain rach during attenuation. | the equine herpesvirus type-1 modified live-vaccine strain rach (256th passage on porcine embryonic kidney cells) was investigated by restriction-enzyme analysis and compared to representative plaque isolates of the 12th passage (racl11, racl22) and 185th passage (racm24, racm36). the restriction patterns of all rac plaque isolates differed compared with reference strain ab4. the left ul terminus was shortened by 0.1 kbp and a missing bamhi site led to the fusion of the f and t fragments. in som ... | 1996 | 8919964 |
| herpesviral diseases affecting reproduction in the horse. | | 1980 | 6262988 |
| replication of equid herpesvirus-1 (ehv-1) in the testes and epididymides of ponies and venereal shedding of infectious virus. | six welsh mountain pony colts were infected intranasally with the ab4 isolate of ehv-1. clinical and virological monitoring demonstrated mild upper respiratory tract disease, with nasal shedding of virus and establishment of a cell-associated viraemia. detailed pathological examination of the urogenital tract was performed post mortem on days 4-9 post-infection (pi). ehv-1 was isolated from the epididymis on day 8 and the testis on day 9 pi, with viral replication in endothelial cells of these o ... | 1996 | 9004080 |
| direct detection of equine herpesvirus dna in tissues of aborted equine fetuses. | restriction endonuclease analysis of equine herpesviruses 1 (ehv-1) and 4 has been investigated using cultured cells infected with these viruses. the dna cleavage patterns of these viruses were observed in the intracellular dna after digestion with eco ri and electrophoresis. this procedure was applied to the diagnosis of equine herpesvirus infection in aborted equine fetuses. the characteristic eco ri restriction pattern of ehv-1 dna was directly detectable in the emulsion of lungs collected fr ... | 1996 | 9011160 |
| analysis of the contributions of the equine herpesvirus 1 glycoprotein gb homolog to virus entry and direct cell-to-cell spread. | experiments to analyze the functions of the equine herpesvirus 1 (ehv-1) glycoprotein gb were performed. cell lines which stably expressed either the full-length ehv-1 gb or only the extracellular portion of gb (amino acids 1 to 844) were constructed and were termed tcgbf and tcgb delta, respectively. using the cell line tcgbf, a gb-negative viral mutant, l11delta gb, was generated by replacing a 2.1-kb bglii-nrui fragment in the ehv-1 strain racl11 gb with the escherichia coli lacz gene. ehv-1 ... | 1997 | 9018127 |
| lack of virulence of the murine fibroblast adapted strain, kentucky a (kya), of equine herpesvirus type 1 (ehv-1) in young horses. | the virulence of the cell culture adapted kya strain of equine herpesvirus type 1 (ehv-1), which lacks at least six genes by deletions in its genome, was assessed by intranasal inoculation of six young horses that were serologically negative for ehv-1. no horses showed clinical signs, and a neutralizing antibody response against ehv-1 was detected in two horses which had antibodies against ehv-4 prior to the inoculation. a challenge experiment using a highly virulent strain of ehv-1 conducted 4 ... | 1996 | 9054131 |
| the gene downstream of the gc homologue in feline herpes virus type 1 is involved in the expression of virulence. | feline herpesvirus type 1 (fhv-1) mutants were constructed, carrying a beta-galactosidase marker gene integrated into the region downstream of the gene encoding the homologue of glycoprotein c (gc) of herpes simplex virus type 1. in cell culture, no differences in replication were observed between mutants and the parent fhv-1 strain. however, in experimentally infected cats, mutants caused fewer clinical signs after oronasal administration although they replicated to the same extent as the paren ... | 1994 | 7964620 |
| immunogenicity of equine herpesvirus type 1 (ehv1) and equine rhinovirus type 1 (erhv1) following inactivation by betapropiolactone (bpl) and ultraviolet (uv) light. | some kinetic data on the inactivation of equine herpesvirus type 1 (ehv1) and equine rhinovirus type 1 (erhv1) by betapropiolactone (bpl) and ultraviolet (uv) irradiation are reported. 0.25% bpl at 37 degrees c for 1 h reduced the titre of ehv1 by greater than 10(3 . 4) and of erhv1 by greater than 10(4 . 1) tcid50/ml. uv irradiation (334 microw/cm2) produced similar reductions in titre after 2 min. these data were used as a basis for inactivating ehv1 and erhv1 by the combined action of bpl and ... | 1982 | 6301141 |
| in vivo harboring of equine herpesvirus-1 in leukocyte populations and subpopulations and their quantitation from experimentally infected ponies. | | 1983 | 6309042 |
| herpesviral abortion in domestic animals. | abortion or neonatal disease may follow infection with several alpha, beta and gamma-herpesviruses. the alpha-herpesvirus, equid herpesvirus-1 (ehv-1), causes single or epizootic abortions or neonatal deaths in equids, and the closely related virus ehv-4 causes sporadic equine abortions. in cattle, the alpha-herpesviruses, bovine herpesvirus-1 (infectious bovine rhinotracheitis virus) and bovine herpesvirus-5 (bovine encephalitis virus), and a gamma-herpesvirus, bovine herpesvirus-4, have all be ... | 1997 | 9232116 |
| equine immunology 4: vaccines and antisera. | this paper attempts to relate the practicalities of vaccine development to the ideals which should be aimed for in a new vaccine. the type of immune response induced is dependent upon the nature of the antigen in the vaccine and the site and timing of its presentation to the immune system. in this respect the influence of age, maternal immunity and antigenic competition are discussed. the possible side effects associated with vaccination are defined and vaccines which are currently available for ... | 1983 | 6309511 |
| use of lambda gt11 to identify antigenic components of equine herpesvirus 4. | a library of the equine herpesvirus 4 (ehv-4) genome was constructed in the lambda gt11 expression vector. recombinant bacteriophage expressing ehv-4 antigens as beta-galactosidase fusion proteins were detected with rabbit antiserum raised against ehv-4 virions and convalescent horse serum. ehv-4 dna sequences contained in the immunopositive recombinants were used as hybridization probes for mapping the genes encoding the antigens on the viral genome. the dna sequence of the probes was determine ... | 1994 | 7521096 |
| antibodies against equine herpesviruses in free-ranging mountain zebras from namibia. | twenty-one blood samples of free-ranging mountain zebras (equus zebra) from namibia were tested for equine herpesvirus (ehv-1, -2, -3, -4) specific antibodies by immunofluorescence assay (ifa) and neutralization test (nt). additionally, type-specific nested polymerase chain reactions (nested pcr) were employed for detection of ehv-1, -2 and -4 dna. equine herpesvirus-1 antibodies were detected by ifa in all zebras, while only seven serum samples contained ehv-4 ifa antibodies. sera with high ifa ... | 1997 | 9391966 |
| application of an equine herpesvirus 1 (ehv1) type-specific elisa to the management of an outbreak of ehv1 abortion. | sera from 33 australian thoroughbred mares were tested during an outbreak of equine herpesvirus 1 (ehv1) abortion with an enzyme-linked immunosorbant assay (elisa) for the presence of ehv1-specific antibodies. the elisa used a recombinant ehv1 antigen derived from glycoprotein g (gg) and distinguished antibodies to ehv1 from those of the antigenically related and widespread herpesvirus ehv4. sera were obtained from most of the mares on three occasions, three, 13 and 67 days after the first abort ... | 1995 | 7571249 |
| an equine herpesvirus type 1 recombinant with a deletion in the ge and gi genes is avirulent in young horses. | the cell culture-adapted kya strain of equine herpesvirus type 1 (ehv-1) has been found to be attenuated in young horses (matsumura et al., 1996, vet. microbiol. 48, 353-365). the kya strain lacks at least six genes in its genome, including those encoding glycoproteins ge and gi. to elucidate whether ehv-1 glycoproteins ge and gi play a role in viral virulence, we have constructed an ehv-1 recombinant that has the genes encoding both ge and gi deleted from its genome and its revertant. growth pr ... | 1998 | 9501037 |
| nucleotide sequences of glycoprotein i and e genes of equine herpesvirus type 4. | the nucleotide sequences of the glycoprotein i (gi) and e (ge) genes of equine herpesvirus type 4 (ehv-4) strain th20 were determined. the predicted region encoding the ehv-4 gi gene is 1,263 nucleotides, corresponding to a polypeptide of 420 amino acids in length. the predicted region encoding the ehv-4 ge gene is 1,647 nucleotides, corresponding to a polypeptide of 548 amino acids in length. the ehv-4 gi and ge genes show 74% and 85% identity at the amino acid level with those of equine herpes ... | 1998 | 9524947 |
| genomic organization of the canine herpesvirus us region. | canine herpesvirus (chv) is an alpha-herpesvirus of limited pathogenicity in healthy adult dogs and infectivity of the virus appears to be largely limited to cells of canine origin. chv's low virulence and species specificity make it an attractive candidate for a recombinant vaccine vector to protect dogs against a variety of pathogens. as part of the analysis of the chv genome, the authors determined the complete nucleotide sequence of the chv us region as well as portions of the flanking inver ... | 1998 | 9620207 |
| guidelines for vaccination of horses. the american association of equine practitioners' vaccination guidelines subcommittee of the avma council on biologic and therapeutic agents. | | 1995 | 7591939 |
| transcriptional analyses of the unique short segment of ehv-1 strain kentucky a. | the unique short (us) segment of the genome of equine herpesvirus type 1 (ehv-1) strain kya is comprised of six open reading frames (orfs) that encode: a) a homolog of the us2 protein of herpes simplex virus type 1 (hsv-1); b) a serine threonine protein kinase that is a homolog of the hsv-1 us3 protein; c) a homolog of pseudorabies virus glycoprotein gx and hsv-2 gg; d) a novel glycoprotein, eus4, not encoded by other herpesviruses sequenced to date; e) a homolog of hsv-1 gd; and f) a homolog of ... | 1995 | 7597804 |
| herpesvirus myeloencephalopathy in horses: 11 cases (1982-1996). | to determine results of csf analysis in horses with equid herpesvirus myeloencephalopathy (ehm) and to determine whether results of csf analysis were associated with outcome. | 1998 | 9731262 |
| neuropathological study of gazelle herpesvirus 1 (equine herpesvirus 9) infection in thomson's gazelles (gazella thomsoni). | gazelle herpesvirus (ghv-1), correctly designated as equine herpesvirus 9, is a new type of equine herpesvirus immunologically related to equine herpesvirus 1 (ehv-1). as a sequel to a virological study, the neuropathology of encephalitis caused by ghv-1 in thomson's gazelles (gazella thomsoni) was examined. seven gazelles died with or without neurological symptoms between early september and mid-october in 1993. no gross abnormalities were observed at necropsy, but all animals had non-suppurati ... | 1998 | 9749360 |
| identification and initial characterization of the ir6 protein of equine herpesvirus 1. | the ir6 gene of equine herpesvirus 1 (ehv-1) is a novel gene that maps within each inverted repeat (ir), encodes a potential protein of 272 amino acids, and is expressed as a 1.2-kb rna whose synthesis begins at very early times (1.5 h) after infection and continues throughout the infection cycle (c. a. breeden, r. r. yalamanchili, c.f. colle, and d.j. o'callaghan, virology 191:649-660,1992). to identify the ir6 protein and ascertain its properties, we generated an ir6-specific polyclonal antise ... | 1994 | 8057419 |
| helicase-primase complex of herpes simplex virus type 1: a mutation in the ul52 subunit abolishes primase activity. | the ul52 gene product of herpes simplex virus type 1 (hsv-1) comprises one subunit of a 3-protein helicase-primase complex that is essential for replication of viral dna. the functions of the individual subunits of the complex are not known with certainty, although it is clear that the ul8 subunit is not required for either helicase or primase activity. examination of the predicted amino acid sequence of the ul5 gene reveals the existence of conserved helicase motifs; it seems likely, therefore, ... | 1994 | 8189507 |
| identification of an infectious laryngotracheitis virus gene encoding an immunogenic protein with a predicted m(r) of 32 kilodaltons. | the nucleotide sequence of an infectious laryngotracheitis virus (iltv) gene which maps immediately upstream from the glycoprotein 60 (gp60) gene was determined. the gene, designated p32, encodes a predicted polypeptide of 298 amino acids with an estimated m(r) of 32,000 daltons. the predicted protein sequence has four potential n-glycosylation sites and a signal sequence at the n-terminal region. amino acid residues in the nh2-terminal region of the p32 protein exhibit similarity to glycoprotei ... | 1993 | 8212855 |
| epidemiology of equine herpesvirus abortion: searching for clues to the future. | | 1998 | 9830567 |
| transcriptional control of the equine herpesvirus 1 immediate early gene. | transient expression assays measuring induction of an equine herpesvirus 1 (ehv-1) immediate early (ie) promoter construct, pie beta gal, were used to examine trans-induction of the ie gene (ir1) promoter by superinfection with intact ehv-1 (kentucky a strain), uv-inactivated ehv-1, or ehv-1 stocks highly enriched for defective interfering particles (dips), and by cotransfection with plasmids containing ehv-1 dna fragments. our results confirm reports by others in that the ie promoter can be ind ... | 1993 | 8249301 |
| a novel arrangement of zinc-binding residues and secondary structure in the c3hc4 motif of an alpha herpes virus protein family. | a highly conserved, cysteine-rich region plays a crucial role in the function of a family of regulatory proteins encoded by alpha herpes viruses. the so-called c3hc4 motif spans approximately 60 residues and has been predicted to bind zinc. this motif occurs in a number of other viral and cellular proteins, many of which appear to be involved in some aspect of the regulation of gene expression. we have cloned and expressed in bacteria a portion of immediate-early protein vmw110 of herpes simplex ... | 1993 | 8263911 |
| antigenic and biochemical analysis of gb of herpes simplex virus type 1 and type 2 and of cross-reacting glycoproteins induced by bovine mammillitis virus and equine herpesvirus type 1. | an antiserum was produced to the oligomeric form of glycoprotein b (gb) induced by herpes simplex virus type 1 (hsv-1) strain 17. this antiserum gave a single common precipitin line in agar gel immunodiffusion with hsv-1, hsv-2, bovine mammillitis virus (bmv) and equine herpesvirus type 1 (ehv-1). it also neutralized hsv-1, hsv-2 and bmv but not ehv-1. absorption of the antiserum with excess hsv-2 or bmv antigen resulted in an hsv-1-specific neutralizing antiserum. in immunoprecipitation, two pr ... | 1985 | 2981966 |
| dna-mediated immunization with glycoprotein d of equine herpesvirus 1 (ehv-1) in a murine model of ehv-1 respiratory infection. | dna-mediated immunization was assessed in a murine model of equine herpesvirus 1 (ehv-1) respiratory infection. a single intramuscular injection with plasmid dna encoding ehv-1 glycoprotein d (ehv-1 gd), including its predicted c-terminal membrane anchor sequence, induced a specific antibody response detectable by 2 weeks and maintained through 23 weeks post injection. a second injection at 4 weeks markedly enhanced the antibody response and all ehv-1 gd-injected mice developed neutralizing anti ... | 1999 | 9987159 |
| use of an immunoperoxidase technique to detect equine herpesvirus-1 antigen in formalin-fixed paraffin-embedded equine fetal tissues. | an indirect immunoperoxidase (ip) procedure using the avidin-biotin-peroxidase complex detection technique was developed to detect viral equine herpesvirus-1 (ehv-1) antigen in formalin-fixed paraffin-embedded tissues from aborted equine fetuses. the procedure was applied to liver, lung, and other tissues from 20 cases of confirmed or suspected ehv-1-induced abortions. specific staining was observed in tissue sections from ehv-1-infected fetuses. positive ip staining was present in tissues of 7 ... | 1993 | 8385497 |
| equine herpesvirus type 1 neurological disease and enterocolitis in mature standardbred horses. | | 1993 | 8389601 |
| expression of small regions of equine herpesvirus 1 glycoprotein c in escherichia coli. | a series of truncated equine herpesvirus 1 (ehv1) glycoprotein c (gc) molecules was examined for use as serodiagnostic antigens for ehv1 and ehv4. small regions of ehv1 glycoprotein c, an immunodominant ehv1 glycoprotein, were expressed in escherichia coli as glutathione s-transferase (gst) fusion proteins using the bacterial expression vector pgex-2t. sera obtained from horses, including sera from specific-pathogen-free (spf) foals, following exposure to either ehv1, ehv4 or both viruses were u ... | 1995 | 8545955 |
| high molecular weight polypeptide bands specific for equine herpesvirus 4. | serum neutralisation (sn) and immunoblotting were used in attempts to distinguish between natural infections with the closely related viruses equine herpesvirus 1 (ehv-1) and equine herpes-virus 4 (ehv-4). horse sera (n = 323) collected in 1990 from studs with no experience of ehv-1 abortions as well as 197 sera collected in 1992 from studs with a history of ehv-1 abortions were tested by sn. the two groups differed in the proportion with measurable ehv-1 antibody, the 1992 group being significa ... | 1995 | 8545957 |
| in vitro cytotoxic activity of equine lymphocytes on equine herpesvirus-1 infected allogenic fibroblasts. | the objectives of this study were to: (1) develop a technique to analyze the in vitro cytotoxic activity of lymphocytes from adult horses against equine herpesvirus-1 (ehv-1) infected allogenic equine dermal fibroblasts (edf); (2) evaluate the ability of a 72-h in vitro incubation with interleukin-2 (il-2) to enhance the lymphocytic cytolytic activity against ehv-1 infected edf; (3) compare the cytotoxic activity of lymphocytes isolated from pregnant mares and non-pregnant mares against ehv-1 in ... | 1996 | 8809999 |
| the detection of latency-associated transcripts of equine herpesvirus 1 in ganglionic neurons. | neural tissues from specific pathogen-free ponies that had been experimentally infected with equine herpesvirus 1 (ehv-1) were analysed by in situ hybridization. digoxigenin-labelled ehv-1 bamhi fragments spanning almost the entire ehv-1 genome were hybridized to rna in tissue sections from latently infected trigeminal ganglia. the bamhi e fragment detected ehv-1 rna antisense to gene 63 (hsv-1 homologue icp0) in a small number of neurons. sixteen other bamhi fragments gave negative results in 2 ... | 1995 | 8847517 |
| icp22 and the ul13 protein kinase are both required for herpes simplex virus-induced modification of the large subunit of rna polymerase ii. | herpes simplex virus type 1 (hsv-1) infection alters the phosphorylation of the large subunit of rna polymerase ii (rnap ii), resulting in the depletion of the hypophosphorylated and hyperphosphorylated forms of this polypeptide (known as iia and iio, respectively) and induction of a novel, alternatively phosphorylated form (designated iii). we previously showed that the hsv-1 immediate-early protein icp22 is involved in this phenomenon, since induction of iii and depletion of iia are deficient ... | 1999 | 10364308 |
| epidemiological studies of equine herpesvirus 1 (ehv-1) in thoroughbred foals: a review of studies conducted in the hunter valley of new south wales between 1995 and 1997. | sero-epidemiological studies conducted between 1995 and 1997 on two large thoroughbred stud farms in the hunter valley of nsw showed clear evidence of ehv-1 infection in foals as young as 30 days of age. similarly, serological evidence suggested that these foals were infected with ehv-1 from their dams or from other lactating mares in the group, with subsequent foal to foal spread of infection prior to weaning. these studies also provided evidence of ehv-1 infection of foals at and subsequent to ... | 1999 | 10501158 |
| association of herpes simplex virus regulatory protein icp22 with transcriptional complexes containing eap, icp4, rna polymerase ii, and viral dna requires posttranslational modification by the u(l)13 proteinkinase. | the expression of herpes simplex virus 1 gamma (late) genes requires functional alpha proteins (gamma1 genes) and the onset of viral dna synthesis (gamma2 genes). we report that late in infection after the onset of viral dna synthesis, cell nuclei exhibit defined structures which contain two viral regulatory proteins (infected cell proteins 4 and 22) required for gamma gene expression, rna polymerase ii, a host nucleolar protein (eap or l22) known to be associated with ribosomes and to bind smal ... | 1997 | 8995634 |
| demonstration of equine herpesvirus-1 neuronal latency in murine olfactory bulbs using a novel combined in situ pcr and protein synthesis method. | equine herpesvirus-1 (ehv-1) latency in murine olfactory bulbs was demonstrated by a novel combined in situ pcr and in vitro protein synthesis method (in situ ps-pcr). the escherichia coli lacz gene replacing a deletion in ehv-1 gene 71 (eus4) was thus amplified and transcribed/translated in situ followed by enzymatic detection using x-gal (5-bromo-4-chloro-3-indoyl-beta-d-galactopyranoside). beta-galactosidase was found to be concentrated over mitral/tufted neurons indicating those to be the si ... | 1997 | 9123871 |
| immunohistochemical demonstration of equine herpesvirus-1 antigen in neurons and astrocytes of horses with acute paralysis. | equine herpesvirus-1 (ehv-1) infection in a few widely scattered neurons and astrocytes plus endothelial cells in brain and spinal cord of two horses with naturally occurring paralytic disease was demonstrated by use of an immunoperoxidase technique. these horses were euthanatized less than 48 hours after the onset of clinical signs. no staining for ehv-1 was demonstrated in brain or spinal cord of three horses that had a longer duration of clinical disease or in two uninfected horses. | 1997 | 9150548 |
| synthesis and processing of the equine herpesvirus 1 glycoprotein m. | in a previous report, the function of the equine herpesvirus 1 (ehv-1) glycoprotein m (gm) homolog was investigated. it was shown that ehv-1 gm is involved in both virus entry and direct cell-to-cell spread of infection (n. osterrieder et al., j. virol. 70, 4110-4115, 1996). in this study, experiments were conducted to analyze the synthesis, posttranslational processing, and the putative ion channel function of ehv-1 gm. it was demonstrated that ehv-1 gm is synthesized as an mr 44,000 polypeptid ... | 1997 | 9185606 |
| high level expression of equine herpesvirus 1 glycoproteins d and h and their role in protection against virus challenge in the c3h (h-2kk) murine model. | n and c-terminal truncated forms of equine herpesvirus 1 (ehv 1) glycoproteins gd and gh were expressed in baculovirus resulting in the production of secreted recombinant proteins. a carboxy-terminal histidine tag was included on each of the genes for protein isolation by nickel affinity chromatography. recombinant gd was recognized by three gd specific monoclonal antibodies, 20c4, 5h6 and f3132. f3132 is a conformationally dependent monoclonal antibody with virus neutralizing activity. expressi ... | 1997 | 9282781 |
| the nucleotidylylation of herpes simplex virus 1 regulatory protein alpha22 by human casein kinase ii. | the products of the alpha genes of herpes simplex virus 1, the infected cells proteins (icp) 0, 4, 22, and 27 perform regulatory functions, are nucleotidylylated, and share the signaling or recognition sequence (rr(a/t)(p/s)r) that correctly predicted the nucleotidylylation of viral proteins encoded by ul21, ul31, ul49, and ul47 genes expressed later in infection. extracts from uninfected hela cells or casein kinase ii purified from sea star nucleotidylylated the icp22 moiety of a glutathione s- ... | 1997 | 9312161 |
| sequencing of a 5.5-kb dna fragment and identification of a gene coding for a subunit of the helicase/primase complex of avian laryngotracheitis virus (iltv). | the nucleotide sequence of a 5,520-bp ecori restriction fragment of avian infectious laryngotracheitis (iltv) dna was reported and submitted to genebank with an accession number of af001078. computer prediction revealed one large potential open reading frame (orf) with sequence similar to one subunit of the dna helicase-primase complex of alpha-herpesviruses. the dna helicase/primase complex of hsv-1 consists of three sub-units with molecular weights of 12,000, 97,000 and 70,000, encoded by gene ... | 1997 | 9421876 |
| phosphorylation of structural components promotes dissociation of the herpes simplex virus type 1 tegument. | the role of phosphorylation in the dissociation of structural components of the herpes simplex virus type 1 (hsv-1) tegument was investigated, using an in vitro assay. addition of physiological concentrations of atp and magnesium to wild-type virions in the presence of detergent promoted the release of vp13/14 and vp22. vp1/2 and the ul13 protein kinase were not significantly solubilized. however, using a virus with an inactivated ul13 protein, we found that the release of vp22 was severely impa ... | 1998 | 9696804 |
| diversity of genomic electropherotypes of naturally occurring equine herpesvirus 1 isolates in argentina. | the genomes of 10 equine herpesvirus 1 (ehv-1) strains isolated in argentina from 1979 to 1991, and a japanese hh1 reference strain were compared by restriction endonuclease analysis. two restriction enzymes, bamhi and bglii, were used and analysis of the electropherotypes did not show significant differences among isolates obtained from horses with different clinical signs. this suggests that the ehv-1 isolates studied, which circulated in argentina for more than 10 years, belong to a single ge ... | 1998 | 9698821 |
| protective immunity against equine herpesvirus type-1 (ehv-1) infection in mice induced by recombinant ehv-1 gd. | the ability of recombinant preparations of equine herpesvirus type 1 (ehv-1) glycoprotein d (gd) to elicit specific antibody and t lymphocyte responses in the balb/c mouse model of respiratory infection was investigated. recombinant gd (rgd) expressed as a glutathione-s-transferase (gst) fusion protein in escherichia coli elicited both high titer neutralizing antibody (nab) and cd4 t cell proliferative responses following subcutaneous or intranasal immunization, but elicited only a weak antibody ... | 1998 | 9784062 |
| mapping the sequences that mediate interaction of the equine herpesvirus 1 immediate-early protein and human tfiib. | the sole immediate-early (ie) gene of equine herpesvirus 1 encodes a 1,487-amino-acid (aa) regulatory phosphoprotein that independently activates expression of early viral genes. coimmunoprecipitation assays demonstrated that the ie protein physically interacts with the general transcription factor tfiib. using a variety of protein-binding assays that employed a panel of ie truncation and deletion mutants expressed as in vitro-synthesized or glutathione s-transferase fusion proteins, we mapped a ... | 2001 | 11581390 |
| the equine herpesvirus 1 ir6 protein that colocalizes with nuclear lamins is involved in nucleocapsid egress and migrates from cell to cell independently of virus infection. | the equine herpesvirus 1 (ehv-1) ir6 protein forms typical rod-like structures in infected cells, influences virus growth at elevated temperatures, and determines the virulence of ehv-1 rac strains (osterrieder et al., virology 226:243-251, 1996). experiments to further elucidate the functions and properties of the ir6 protein were conducted. it was shown that the ir6 protein of wild-type racl11 virus colocalizes with nuclear lamins very late in infection as demonstrated by confocal laser scan m ... | 1998 | 9811716 |
| diagnosis and sero-epizootiology of equine herpesvirus type 1 and type 4 infections in japan using a type-specific elisa. | recently, a type-specific elisa using equine herpesvirus type 1 (ehv-1) and type 4 (ehv-4) glycoprotein gs (ggs) was developed by crabb and studdert [1993]. to investigate the dissemination of ehv-1 and -4 among horses in japan, we applied their elisa as suitable for discriminating between ehv-1 and -4 infections serologically. type-specificity of the elisa was confirmed by using paired sera of infected horses with either ehv-1 or -4. application of the elisa to sera collected before and after t ... | 1998 | 9819768 |
| systemic infection by equid herpesvirus-1 in a grevy's zebra stallion (equus grevyi) with particular reference to genital pathology. | a severe multi-systemic form of equid herpesvirus-1 infection is described in an adult zebra stallion. there was multifocal necrotizing rhinitis, marked hydrothorax and pulmonary oedema, with viral antigen expression in degenerating epithelial cells, local endothelial cells and intravascular leucocytes of the nasal mucosa and lung. specific localization of ehv-1 infection was seen in the testes and epididymides, including infection of leydig cells and germinal epithelium, which would have facili ... | 1998 | 9839210 |
| an equine herpesvirus 1 mutant with a lacz insertion between open reading frames 62 and 63 is replication competent and causes disease in the murine respiratory model. | an equine herpesvirus 1 (ehv-1) mutant was constructed by inserting a lacz expression cassette into the intergenic region upstream of gene 62 (glycoprotein l; gl) and downstream of gene 63 (a homologue of the herpes simplex virus transcriptional activator icp0). the recombinant lacz62/63-ehv-1 had similar growth kinetics in cell culture to those of the parental wild type (wt) virus, with indistinguishable cytopathic effects and plaque morphology. reverse transcriptase pcr confirmed that the lacz ... | 1998 | 9856103 |
| increased susceptibility of peripheral blood mononuclear cells to equine herpes virus type 1 infection upon mitogen stimulation: a role of the cell cycle and of cell-to-cell transmission of the virus. | equine herpesvirus-1 (ehv-1) is an important pathogen of horses, causing abortion and nervous system disorders, even in vaccinated animals. during the cell-associated viremia, ehv-1 is carried by peripheral blood mononuclear cells (pbmc), mainly lymphocytes. in vitro, monocytes are the most important fraction of pbmc in which ehv-1 replicates, however, mitogen stimulation prior to ehv-1 infection increases the percentage of infected lymphocytes. the role of the cell cycle in viral replication an ... | 2002 | 11888698 |
| ehv-1 eicp22 protein sequences that mediate its physical interaction with the immediate-early protein are not sufficient to enhance the trans-activation activity of the ie protein. | the early 293 amino acid eicp22 protein (eicp22p) of equine herpesvirus 1 localizes within the nucleus and functions as an accessory regulatory protein (j. virol. 68 (1994) 4329). transient transfection assays indicated that although the eicp22p by itself only minimally trans-activates ehv-1 promoters, the eicp22p functions synergistically with the immediate-early protein (iep) to enhance expression of ehv-1 early genes (j. virol. 71 (1997) 1004). we previously showed that the eicp22 protein enh ... | 2002 | 11900834 |
| equine herpesvirus type 1 (ehv-1) myeloencephalopathy: a case report. | an outbreak of neurological disease occurred in a well-managed riding school. ataxia and paresis were observed in several horses, five of which became recumbent and were euthanized. post-mortem analysis revealed scattered haemorrhages along the spinal cord, that were reflected by multiple haemorrhagic foci on formalin-fixed sections, with the thoracic and lumbar segments being the most affected. pathohistologically, perivascular mononuclear cuffing and axonal swelling, especially in the white ma ... | 2002 | 11911591 |
| the c-terminal regions of the envelope glycoprotein gp2 of equine herpesviruses 1 and 4 are antigenically distinct. | the unusual mucin-like high molecular mass (mr) glycoprotein 2 (gp2) has only been described in the equid alphaherpesviruses, among which there is considerable antigenic cross-reactivity. equine herpesvirus 1 (ehv-1) gp2 is cleaved into a highly glycosylated n-terminal subunit and a 42 kda c-terminal cleavage product. in order to investigate their antigenic recognition by horses naturally infected with ehv-1 and/or equine herpesvirus 4 (ehv-4), the c-terminal cleavage product and high mr gp2 wer ... | 2002 | 11958459 |
| herpesvirus icp18.5 and dna-binding protein genes are conserved in equine herpesvirus-1. | the genome of equine herpesvirus-1 (ehv-1) contained three open reading frames (orfs) in a 3.9 kbp bamhi-smai fragment at 0.38-0.41 map units in the long unique region. the most 5' orf encoded the carboxy terminus of a protein with 45-55 percent amino acid homology to the dna-binding proteins (icp8-dbp) of four other alpha-herpesviruses. the middle orf translated to a polypeptide of 775 residues with 43-55% homology to the icp18.5 proteins. the most 3' orf encoded the ehv-1 glycoprotein b (gb) g ... | 1993 | 8279122 |
| inter- and intra-strain genomic variation in equine herpesvirus type 1 isolates. | restriction enzyme digests of dna from 22 unselected isolates of ehv-1 were analysed by hybridization with cloned dna fragments covering the genome. in addition to a small amount of inter-strain variation, heterogeneity within strains was observed, caused by loss of specific restriction endonuclease sites in the dna of a proportion of the virus particles of any one stock. fifteen strains demonstrated the same intra-strain variation involving loss of the bamhi l-m site which was shown to lie with ... | 1994 | 8279952 |
| equid herpesvirus 1 infection of endothelial cells requires activation of putative adhesion molecules: an in vitro model. | antisera to activated equine endothelial cells, which detected surface molecules of 116 kd, 97 kd, 42 kd and 38 kd, were made to investigate the role of endothelial adhesion molecules in equid herpes virus 1 infection. these putative adhesion molecules could be induced by 17-beta oestradiol, chorionic gonadotrophin, or il-2, as well as by lps and pwm. in an in vitro flow system, using equine veins or arteries, equid herpesvirus 1 in leucocytes was only transferred to infect endothelial cells if ... | 2002 | 12165084 |
| [vaccination against rhinopneumonia]. | | 1994 | 8291048 |
| construction and characterization of an equine herpesvirus 1 glycoprotein c negative mutant. | an equine herpesvirus 1 (ehv-1) strain racl 11 mutant was constructed that carries the escherichia coli lacz gene instead of the open reading frame encoding glycoprotein c (gc). the engineered virus mutant (l11(delta)gc) lacked codons 46-440 of the 1404 bp gene. on rabbit kidney cell line rk13 and equine dermal cell line edmin337, the l11(delta)gc virus grew to titers which were reduced by approximately 5- to 10-fold compared with wild-type racl11 virus or a repaired virus (r-l11(delta)gc). howe ... | 1999 | 10082388 |
| infection with equine herpesvirus 1 (ehv-1) strain hvs25a in pregnant mice. | the abortigenic effects of equine herpesvirus 1 (ehv-1) strain hvs25a, given intranasally, were assessed in pregnant balb/c, c57bl/6j and quakenbush mice at day 16 of pregnancy. all ehv-1-infected balb/c mice showed clinical signs typical of ehv-1-induced disease, together with evidence of abortion. however, although there were fetal and neonatal deaths in some c57bl/6j and quakenbush litters, the respiratory and systemic effects of ehv-1 infection in the dams were inconsistent. balb/c dams were ... | 1999 | 10098013 |
| the gamma2 late glycoprotein k promoter of equine herpesvirus 1 is differentially regulated by the ie and eicp0 proteins. | the equine herpesvirus 1 immediate-early (ie) phosphoprotein is essential for the activation of transcription from viral early and late promoters and trans-represses its own promoter. transient-transfection assays showed that the ie protein trans-represses the gamma2 late gk promoter. gel shift and dnase i footprinting assays demonstrated that the ie protein binds to the gk promoter sequences from -42 to -26 and from -13 to +12 that overlap the transcription initiation site (+1). these results i ... | 1999 | 10191181 |