Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| neuropathogenic and non-neuropathogenic variants of equine herpesvirus 1 in france. | equine herpesvirus 1 (ehv-1) is a common pathogen of the horse which may induce mild respiratory distress, abortion, neonatal death and neurological disease. a single nucleotide polymorphism in the ehv-1 dna polymerase (orf30 a(2254) to g(2254)) has been associated with clinical signs of equine herpes myeloencephalopathy (ehm). the aim of this work was to analyze the orf30 genomic region among a panel of ehv-1 dna extract in order to estimate the prevalence of the ehv-1 neuropathogenic genotype ... | 2010 | 20427133 |
| molecular investigation of the viral kinetics of equine herpesvirus-1 in blood and nasal secretions of horses after corticosteroid-induced recrudescence of latent infection. | recrudescence of latent equine herpesvirus 1 (ehv-1) with subsequent viral shedding via nasal secretions is a potential source of infection for susceptible horses and has been implicated in outbreaks occurring in closed populations. | 2010 | 20584139 |
| equus caballus major histocompatibility complex class i is an entry receptor for equine herpesvirus type 1. | in this study, equus caballus major histocompatibility complex class i (mhc-i) was identified as a cellular entry receptor for the alphaherpesvirus equine herpesvirus type 1 (ehv-1). this novel ehv-1 receptor was discovered using a cdna library from equine macrophages. cdnas from this ehv-1-susceptible cell type were inserted into ehv-1-resistant b78h1 murine melanoma cells, these cells were infected with an ehv-1 lacz reporter virus, and cells that supported virus infection were identified by x ... | 2010 | 20610718 |
| antibodies to pathogenic livestock viruses in a wild vicuña (vicugna vicugna) population in the argentinean andean altiplano. | serum samples from 128 wild vicuñas (vicugna vicugna) were tested for antibodies (ab) to rotavirus (rv), bovine parainfluenza virus 3 (bpiv-3), bovine herpesvirus-1 (bhv-1), bovine viral diarrhea virus (bvdv-1), foot-and-mouth disease virus (fmdv), bluetongue virus (btv), equine herpesvirus-1 (ehv-1), and influenza a virus equine (eiv). samples were collected in cieneguillas province of jujuy, in northern argentina. feces from 44 vicuñas were also collected to investigate rv shedding. llamas (la ... | 2010 | 20688660 |
| infection of central nervous system endothelial cells by cell-associated ehv-1. | infection with equine herpesvirus-1 (ehv-1) causes respiratory disease, late-term abortions and equine herpesvirus myeloencephalitis (ehm). our understanding of ehm pathogenesis is limited except for the knowledge that ehv-1 infected, circulating peripheral blood mononuclear cells (pbmc) transport virus to the central nervous system vasculature causing endothelial cell infection leading to development of ehm. our objective was to develop a model of cns endothelial cell infection using ehv-1 infe ... | 2010 | 20884134 |
| relationship between equine herpesvirus-1 myeloencephalopathy and viral genotype. | 2010 | 21039794 | |
| induction of a th-1-biased igg subclass response against equine herpesvirus type 1 in horses previously infected with type 4 virus. | an immunoglobulin g (igg) subclass response against equine herpesvirus type 1 (ehv-1) infection was investigated in horses that were naïve to ehv-1/4 and those that had previously been exposed to ehv-4. the igg subclass response was determined by an elisa using ehv-1-specific recombinant gg protein as an antigen. in most horses naïve to ehv-1/4, igga, iggb, and igg(t) were induced after experimental infection with ehv-1. in contrast, a subclass response dominated by igga and iggb, with no appare ... | 2010 | 21139350 |
| experimental infection with neuropathogenic equid herpesvirus type 1 (ehv-1) in adult horses. | equid herpesvirus type 1 (ehv-1)-associated myeloencephalopathy (ehm) may follow an infection with the virus in horses. this study tested three hypotheses: (1) a large inhaled dose of a neuropathogenic ehv-1 strain would induce a cell-associated viraemia in all infected horses; (2) neurological disease will only occur in viraemic horses, and (3) the cerebrospinal fluid (csf) composition following ehv-1 viraemia will be an indicator for ehm. four ehv-1 seronegative horses were inoculated with ehv ... | 2010 | 19726209 |
| the increased prevalence of neuropathogenic strains of ehv-1 in equine abortions. | a panel of 426 archived ehv-1 isolates collected (1951-2006) from equine abortions was analyzed using a real-time taq-man((r)) allelic discrimination pcr assay. based on previous findings, isolates possessing adenine at nucleotide position 2254 (a(2254)) in orf30 were classified as having a non-neuropathogenic genotype and those with guanine at 2254 (g(2254)) were designated as the neuropathogenic genotype. the resultant data demonstrated that viruses with the neuropathogenic genotype existed in ... | 2010 | 19733451 |
| a vectored equine herpesvirus type 1 (ehv-1) vaccine elicits protective immune responses against ehv-1 and h3n8 equine influenza virus. | vaccination is commonly used to control equine respiratory pathogens such as equine herpesvirus type 1 (ehv-1) and equine influenza virus (eiv). here, we describe the generation and characterization of a recombinant ehv-1 modified live virus vaccine (mlv) based on a recent abortogenic ehv-1 strain, ny03. the immunogenicity and efficacy of the mlv was tested in horses in an ehv-1 vaccination/challenge experiment using the highly virulent neurovirulent ehv-1 strain oh03. induction of a robust ehv- ... | 2010 | 19897066 |
| equine herpesvirus type 1 (ehv-1) utilizes microtubules, dynein, and rock1 to productively infect cells. | to initiate infection, equine herpesvirus type 1 (ehv-1) attaches to heparan sulfate on cell surfaces and then interacts with a putative glycoprotein d receptor(s). after attachment, virus entry occurs either by direct fusion of the virus envelope with the plasma membrane or via endocytosis followed by fusion between the virus envelope and an endosomal membrane. upon fusion, de-enveloped virus particles are deposited into the cytoplasm and travel to the nucleus for viral replication. in this rep ... | 2010 | 19713056 |
| equine major histocompatibility complex class i molecules act as entry receptors that bind to equine herpesvirus-1 glycoprotein d. | the endotheliotropism of equine herpesvirus-1 (ehv-1) leads to encephalomyelitis secondary to vasculitis and thrombosis in the infected horse central nervous system (cns). to identify the host factors involved in ehv-1 infection of cns endothelial cells, we performed functional cloning using an equine brain microvascular endothelial cell cdna library. exogenous expression of equine major histocompatibility complex (mhc) class i heavy chain genes conferred susceptibility to ehv-1 infection in mou ... | 2011 | 21306483 |
| the ul4 protein of equine herpesvirus 1 is not essential for replication or pathogenesis and inhibits gene expression controlled by viral and heterologous promoters. | defective interfering particles (dip) of equine herpesvirus 1 (ehv-1) inhibit standard virus replication and mediate persistent infection. the dip genome is comprised of only three genes: ul3, ul4, and a hybrid gene composed of portions of the ir4 (eicp22) and ul5 (eicp27) genes. the hybrid gene is important for dip interference, but the function(s) of the ul3 and ul4 genes are unknown. here, we show that ul4 is an early gene activated solely by the immediate early protein. the ul4 protein (ul4p ... | 2011 | 21324502 |
| equine alphaherpesviruses (ehv-1 and ehv-4) differ in their efficiency to infect mononuclear cells during early steps of infection in nasal mucosal explants. | equine herpesvirus type 1 (ehv-1) replicates extensively in the epithelium of the upper respiratory tract, after which it can spread throughout the body via a cell-associated viremia in mononuclear leukocytes reaching the pregnant uterus and central nervous system. in a previous study, we were able to mimic the in vivo situation in an in vitro respiratory mucosal explant system. a plaquewise spread of ehv-1 was observed in the epithelial cells, whereas in the connective tissue below the basement ... | 2011 | 21536394 |
| direct detection of equine herpesvirus type 1 dna in nasal swabs by loop-mediated isothermal amplification (lamp). | we evaluated loop-mediated isothermal amplification (lamp) as a means of detecting equine herpesvirus type 1 (ehv-1) dna directly from nasal swabs. to increase the sensitivity, we added a step in which the samples were heat-treated to the original lamp procedure. the detection limit of the lamp assay with heat treatment was 10 times more sensitive than the original lamp assay even when the dna extraction step was omitted. in addition, the lamp assay with heat treatment was more sensitive than th ... | 2011 | 21551979 |
| identification of functional domains of the ir2 protein of equine herpesvirus 1 required for inhibition of viral gene expression and replication. | the equine herpesvirus 1 (ehv-1) negative regulatory ir2 protein (ir2p), an early 1,165-amino acid (aa) truncated form of the 1487-aa immediate-early protein (iep), lacks the trans-activation domain essential for iep activation functions but retains domains for binding dna, tfiib, and tbp and the nuclear localization signal. ir2p mutants of the n-terminal region which lack either dna-binding activity or tfiib-binding activity were unable to down-regulate ehv-1 promoters. in ehv-1-infected cells ... | 2011 | 21794889 |
| structural and functional analysis of the tap-inhibiting ul49.5 proteins of varicelloviruses. | viral infections are counteracted by virus-specific cytotoxic t cells that recognize the infected cell via mhc class i (mhc i) molecules presenting virus-derived peptides. the loading of the peptides onto mhc i molecules occurs in the endoplasmic reticulum (er) and is facilitated by the peptide loading complex. a key player in this complex is the transporter associated with antigen processing (tap), which translocates the viral peptides from the cytosol into the er. herpesviruses have developed ... | 2011 | 21764135 |
| molecular data of ul24 homolog gene (orf37) from brazilian isolates of equine herpesvirus type 1. | equine herpesvirus type 1 (ehv-1) is associated with abortions, respiratory distress, and neurological disturbances in horses. the orf37 of ehv-1 encodes a protein homolog to ul24 gene product of human herpesvirus that has been associated with neurovirulence. in the present work, orf37 pcr fragments derived from two brazilian ehv-1 isolates, a german isolate and an american reference strain were sequenced and characterized by molecular phylogenetic analysis. this genomic region is highly conserv ... | 2011 | 21684566 |
| Equine herpesvirus type 1 (EHV-1) mediated oncolysis of human glioblastoma multiforme cells. | The cytolytic animal virus equine herpesvirus type 1 (EHV-1) was evaluated for its oncolytic potential against five human glioblastoma cell lines. EHV-1 productively infected four of these cell lines and the degree of infection was positively correlated with glioma cell death. No human MHC-I was detected in the resistant glioma line, while infection of the susceptible glioma cell lines, which expressed human MHC-I, were blocked with antibody to MHC-I, indicating that human MHC-I acts as an EHV-1 ... | 2011 | 22205738 |
| latent equine herpesvirus-1 in trigeminal ganglia and equine idiopathic headshaking. | trigeminal neuralgia or neuropathic pain has been regarded as a putative cause of idiopathic headshaking in horses. equine herpesvirus-1 (ehv-1) infection and resultant postherpetic pain have been suggested as a possible cause of such neuropathic pain. | 2012 | 22211434 |
| the common equine class i molecule eqca-1*00101 (ela-a3.1) is characterized by narrow peptide binding and t cell epitope repertoires. | here we describe a detailed quantitative peptide-binding motif for the common equine leukocyte antigen (ela) class i allele eqca-1*00101, present in roughly 25 % of thoroughbred horses. we determined a preliminary binding motif by sequencing endogenously bound ligands. subsequently, a positional scanning combinatorial library (pscl) was used to further characterize binding specificity and derive a quantitative motif involving aspartic acid in position 2 and hydrophobic residues at the c-terminus ... | 2015 | 26399241 |
| long term stability and infectivity of herpesviruses in water. | for viruses to utilize environmental vectors (hard surfaces, soil, water) for transmission, physical and chemical stability is a prerequisite. there are many factors including ph, salinity, temperature, and turbidity that are known to contribute to the ability of viruses to persist in water. equine herpesvirus type-1 (ehv-1) is a pathogenic alphaherpesvirus associated with domestic horses and wild equids. ehv-1 and recombinants of ehv-1 and ehv-9 are able to cause infections in non-equid animal ... | 2017 | 28429732 |
| host and viral traits predict zoonotic spillover from mammals. | the majority of human emerging infectious diseases are zoonotic, with viruses that originate in wild mammals of particular concern (for example, hiv, ebola and sars). understanding patterns of viral diversity in wildlife and determinants of successful cross-species transmission, or spillover, are therefore key goals for pandemic surveillance programs. however, few analytical tools exist to identify which host species are likely to harbour the next human virus, or which viruses can cross species ... | 2017 | 28636590 |
| a point mutation in a herpesvirus co-determines neuropathogenicity and viral shedding. | a point mutation in the dna polymerase gene in equine herpesvirus type 1 (ehv-1) is one determinant for the development of neurological disease in horses. three recently conducted infection experiments using domestic horses and ponies failed to detect statistically significant differences in viral shedding between the neuropathogenic and non-neuropathogenic variants. these results were interpreted as suggesting the absence of a consistent selective advantage of the neuropathogenic variant and th ... | 2017 | 28075374 |