Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| molecular cloning and expression of cu/zn-containing superoxide dismutase from fasciola hepatica. | the cytosolic superoxide dismutase (sod) of fasciola hepatica, a causative agent of fascioliasis, was purified and characterized. the enzyme consists of two identical subunits, each with an apparent molecular mass of 17.5 kda. an analysis of the enzyme's primary structure and inhibition studies revealed that the enzyme is a copper/zinc-containing sod (cu/zn-sod). the enzyme activity was relatively stable in a broad ph range, from ph 7.0 to 10.0, and the enzyme showed maximum activity at ph 7.5. ... | 2000 | 10858207 |
| the genetic diversity of predominant escherichia coli strains isolated from cattle fed various amounts of hay and grain(1). | when the 16s rdna of predominant escherichia coli strains from cattle was digested with hhai and haeiii, the strains could be sub-divided into four operational taxonomic units. when genomic dna was digested with xbai, strains could be grouped into 24 pulsed-field gel electrophoresis genotypes (>95% dice similarity) and five clades (>20% dice similarity). diet (hay versus grain) and gastrointestinal compartment (rumen versus colon) did not have a large impact on diversity. however, both analyses ... | 2000 | 10858581 |
| interactions of the nadp(h)-binding domain iii of proton-translocating transhydrogenase from escherichia coli with nadp(h) and the nad(h)-binding domain i studied by nmr and site-directed mutagenesis. | using the purified nadp(h)-binding domain of proton-translocating escherichia coli transhydrogenase (eciii) overexpressed in (15)n- and (2)h-labeled medium, together with the purified nad(h)-binding domain from e. coli (eci), the interface between eciii and eci, the nadp(h)-binding site and the influence on the interface by nad(p)(h) was investigated in solution by nmr chemical shift mapping. mapping of the nadp(h)-binding site showed that the nadp(h) substrate is bound to eciii in an extended c ... | 2000 | 11027139 |
| role of non-o157 vtec. | non-o157 vtec are typical escherichia coli that differ only in their ability to produce verocytotoxins (vt). the transmission of vtec is discussed in relation to the transmission of commensal e. coli. the emergence over the last few decades of a great variety of vtec serotypes from healthy and diseased humans and animals is described. particular attention is given to the distribution of the more important serogroups pathogenic for humans that have been described from around the world, particular ... | 2000 | 10880178 |
| survival of verocytotoxigenic escherichia coli o157 in soil, water and on surfaces. | cattle and sheep are major reservoirs of escherichia coli o157 and consequently these and certain other farm animals can pass out large numbers of this organism in their faeces. thus the ability of the organism to survive in faeces, on pastureland and in associated water systems has important implications for its spread to crops by direct application of manure, by irrigation with infected water or directly to man by contact with animals or contaminated soil. model systems were used to determine ... | 2000 | 10880181 |
| antigenic and genetic characterization of lipoprotein lppq from mycoplasma mycoides subsp. mycoides sc. | lipoprotein lppq, a predominant 48-kda antigen, and its corresponding gene, lppq, were characterized in mycoplasma mycoides subsp. mycoides sc, the etiological agent of contagious bovine pleuropneumonia. the lppq gene is specific to m. mycoides subsp. mycoides sc and was found in the type strain and in field strains isolated in europe, africa, and australia, as well as in vaccinal strains. lppq is encoded as a precursor with a consensus sequence for prokaryotic signal peptidase ii and a lipid at ... | 2000 | 10882657 |
| influence of hmg-1 and adenovirus oncoprotein e1a on early stages of transcriptional preinitiation complex assembly. | the tata-binding protein (tbp) in the tfiid complex binds specifically to the tata-box to initiate the stepwise assembly of the preinitiation complex (pic) for rna polymerase ii transcription. transcriptional activators and repressors compete with general transcription factors at each step to influence the course of the assembly. to investigate this process, the tbp.tata complex was titrated with hmg-1 and the interaction monitored by electrophoretic mobility shift assays. the titration produced ... | 2000 | 10882737 |
| rotational mobility and orientational stability of a transport protein in lipid membranes. | a single-cysteine mutant of the lactose transport protein lacs(c320a/w399c) from streptococcus thermophilus was selectively labeled with a nitroxide spin label, and its mobility in lipid membranes was studied as a function of its concentration in the membrane by saturation-transfer electron spin resonance. bovine rhodopsin was also selectively spin-labeled and studied to aid the interpretation of the measurements. observations of spin-labeled proteins in macroscopically aligned bilayers indicate ... | 2000 | 10920009 |
| chalcone and stilbene synthases expressed in eucaryotes exhibit reduced cross-reactivity in vitro. | chalcone synthase (chs) and stilbene synthase (sts) catalyze different cyclization reactions of the common tetraketide to give different products, naringenin chalcone and resveratrol, respectively. we have previously observed in vitro cross-reaction of chs and sts overexpressed in escherichia coli, resveratrol production by chs and chalcone production by sts. when expressed in eucaryotic cells, or in e. coli as thioredoxin-fusion proteins, chs and sts exhibited reduced cross-reaction. sts refold ... | 2000 | 10923839 |
| inhibition of six serine proteinases of the human coagulation system by mutants of bovine pancreatic trypsin inhibitor. | a series of 12 bovine pancreatic trypsin inhibitor variants mutated in the p(4) and p(3) positions of the canonical binding loop containing additional k15r and m52l mutations were used to probe the role of single amino acid substitutions on binding to bovine trypsin and to the following human proteinases involved in blood clotting: plasmin, plasma kallikrein, factors x(a) and xii(a), thrombin, and protein c. the mutants were expressed in escherichia coli as fusion proteins with the le1413 hydrop ... | 2000 | 10930417 |
| milk leucocyte population patterns in bovine udder infection of different aetiology. | this study compared the different leucocyte populations in milk from udders infected with different mastitic pathogens and in different stages of infection. milk samples were collected from quarters free of intramammary infection, acutely infected with escherichia coli or staphylococcus aureus and chronically infected with s. aureus, coagulase-negative staphylococci (cns) or streptococcus dysgalactiae. udder bacteriological status was confirmed after three consecutive bacteriological examination ... | 2000 | 11075546 |
| p64k meningococcal protein as immunological carrier for weak immunogens. | previously, the p64k meningococcal protein, an antigen of 64 kda expressed in escherichia coli, has been extensively characterized. we have successfully conjugated several synthetic peptides and meningococcal group c polysaccharide to p64k. in three out of four model peptides, the murine humoral immune response against the homologous peptide, evaluated after three doses of conjugate, was higher in the animals immunized with the coupled peptide than in those that received free peptide. the fourth ... | 2000 | 10931377 |
| isolation of shiga toxin-producing escherichia coli including o157:h7 strains from dairy cattle and beef samples marketed in calcutta, india. | 2000 | 10933264 | |
| coupling efficacy and selectivity of the human mu-opioid receptor expressed as receptor-galpha fusion proteins in escherichia coli. | two constructs encoding the human micro-opioid receptor (hmor) fused at its c terminus to either one of two galpha subunits, galpha(o1) (hmor-galpha(o1)) and galpha(i2) (hmor-galpha(i2)), were expressed in escherichia coli at levels suitable for pharmacological studies (0.4-0.5 pmol/mg). receptors fused to galpha(o1) or to galpha(i2) maintained high-affinity binding of the antagonist diprenorphine. affinities of the micro-selective agonists morphine, [d-ala(2),n-me-phe(4),gly(5)-ol]enkephalin (d ... | 2000 | 10936202 |
| a functional water channel protein in the pathogenic bacterium brucella abortus. | the gene for a new bacterial aquaporin, aqpx, was cloned from the pathogenic gram-negative bacterium brucella abortus. the gene was mapped on the large chromosome of b. abortus. it is flanked by one upstream and two downstream copies of the brucella repeated sequence bru-rs. prediction from the nucleotide sequence indicated that the protein is a member of the mip family, which comprises channels for water and/or solute transport. expression in xenopus oocytes and cryoelectron microscopy of esche ... | 2000 | 11101683 |
| [action of natural gamma-interferons on functional activity of phagocytes and antibody synthesis after vaccination]. | natural swine and cattle gamma-ifns were prepared for trials. one dose of gamma-suiferon contained 1000 iu, that of gamma-boviferon--2000 iu. three series of researches were carried out to estimate the in vitro and in vivo absorbing activity of phagocytes (monocytes and neutrophiles), their bactericidal ability (on new born pigs and calves, 2 months old animals, sows and cows with calf) and antibodygenesis after immunization of animals by colibacteriosis vaccine. it has been shown in trials that ... | 2000 | 11247346 |
| survival of salmonella typhimurium and escherichia coli o157:h7 in poultry manure and manure slurry at sublethal temperatures. | exponential inactivation was observed for salmonella typhimurium and escherichia coli o157:h7 in poultry manure with decimal reduction times ranging from half a day at 37 c to 1-2 wk at 4 c. there was no material difference in inactivation rates between s. typhimurium and e. coli o157:h7. inactivation was slower in slurries made by mixing two parts of water with one part of manure; decimal reduction times (time required for 90% destruction) ranged from 1-2 days at 37 c to 6-22 wk at 4 c. escheri ... | 2000 | 11195639 |
| [detection of escherichia coli strains harboring pathogenicity island of yersinia enterolitica in diarrheal patients and animals in china]. | to investigate the prevalence of escherichia coil strains harboring high pathogenicity island of yersinia enterocolitica in diarrhea patients, animals, food samples and resulted clinical symptoms. | 2000 | 11860774 |
| prevalence of escherichia coli o157:h7 from cull dairy cows in new york state and comparison of culture methods used during preharvest food safety investigations. | a number of protocols for the cultural detection of escherichia coli o157:h7 in clinical fecal specimens have been proposed. in the present study direct plating of cattle feces was compared to three different broth enrichment protocols, i.e., a protocol with modified e. coli broth with novobiocin, a protocol with trypticase soy broth with cefixime and vancomycin, and a protocol with gram-negative broth with novobiocin, for their relative abilities to detect e. coli o157:h7 in feces. in all enric ... | 2000 | 10618108 |
| typing of intimin genes in human and animal enterohemorrhagic and enteropathogenic escherichia coli: characterization of a new intimin variant. | enteropathogenic escherichia coli (epec) and enterohemorrhagic e. coli (ehec) produce the characteristic "attaching and effacing" (a/e) lesion of the brush border. intimin, an outer membrane protein encoded by eae, is responsible for the tight association of both pathogens with the host cell. several eae have been cloned from different epec and ehec strains isolated from humans and animals. these sequences are conserved in the n-terminal region but highly variable in the last c-terminal 280 amin ... | 2000 | 10603369 |
| epidemiological study of pap genes among diarrheagenic or septicemic escherichia coli strains producing cs31a and f17 adhesins and characterization of pap(31a) fimbriae. | the association of the pap operon with the cs31a and f17 adhesins was studied with 255 escherichia coli strains isolated from calves, lambs, or humans with diarrhea. the three classes of papg adhesin with different receptor binding preferences were also screened. the pap operon was associated with 50 and 36% of human strains that produced cs31a and ovine strains that produced f17, respectively. among the bovine isolates, the pap operon was detected in 61% of the cs31a-positive isolates and 72% o ... | 2000 | 10747134 |
| the influence of inorganic phosphate on the activity of adenosine kinase. | the enzyme adenosine kinase (ak; ec 2.7.1.20) shows a dependence upon inorganic phosphate (pi) for activity. the degree of dependence varies among enzyme sources and the ph at which the activity is measured. at physiological ph, recombinant ak from chinese hamster ovary (cho) cells and ak from beef liver (bl) show higher affinities for the substrate adenosine (ado), larger maximum velocities and lower sensitivities to substrate inhibition in the presence of pi. at ph 6.2, both bl and cho ak exhi ... | 2000 | 10606765 |
| inhibitory effects of bovine lactoferrin on the adherence of enterotoxigenic escherichia coli to host cells. | adherence is an essential and prerequisite step for the colonization of mucosal surfaces by enterotoxigenic escherichia coli (etec). we studied the effect of bovine lactoferrin (blf) on the adherence of etec to human epithelial cells in vitro, and to intestinal mucosa of icr germfree mice in vivo. in the in vitro study, blf was found to inhibit the adherence of etec. this adhesion-inhibiting activity of blf was found to lessen with decreasing blf concentration, but the data obtained suggest a po ... | 2000 | 10737192 |
| rapid and specific enzyme immunoassay on hydrophobic grid membrane filter for detection and enumeration of thermophilic campylobacter spp. from milk and chicken rinses. | six commercially available anti-campylobacter antibodies were examined for their applicability in an enzyme immunoassay on hydrophobic grid membrane filters, both for the detection and enumeration of campylobacter spp. when a panel of nine campylobacter (seven campylobacter jejuni and two campylobacter coli) and eight non-campylobacter strains were used in a dot-blot format enzyme immunoassay to test the specificity of these antibodies, only one polyclonal antibody (biodesign) detected all campy ... | 2000 | 10772214 |
| activity of hydrolysed lactoferrin against foodborne pathogenic bacteria in growth media: the effect of edta. | lactoferrin was hydrolysed with pepsin and the antimicrobial activity of the resulting hydrolysate (hlf) was studied in 1% peptone, 0.05% yeast extract, 1% glucose (pyg) medium and tryptic soy broth (tsb). hlf was effective against listeria monocytogenes, enterohaemorrhagic escherichia coli and salmonella enteritidis in pyg, however, the highest studied concentration (1.6 mg ml-1) did not inhibit growth of any of these organisms in tsb. the addition of edta enhanced the activity of hlf in tsb, i ... | 2000 | 10747257 |
| effect of ph on survival, thermotolerance, and verotoxin production of escherichia coli o157:h7 during simulated fermentation and storage. | heat treatment is increasingly being introduced to fermented meat processing, since the acid tolerance properties of escherichia coli o157:h7 can permit this organism to survive traditional processing procedures. this study investigated the effect of growth ph and fermentation on the thermotolerance at 55 degrees c of e. coli o157:h7 in a model fermented meat system. e. coli o157:h7 (strain 380-94) was grown at ph 5.6 or 7.4 (18 h at 37 degrees c), fermented to ph 4.8 or 4.4 in brain heart infus ... | 2000 | 10643763 |
| interventions to reduce microbiological contamination of beef variety meats. | hot water and solutions of acetic acid, lactic acid, or trisodium phosphate applied by immersion or spraying, chlorine solution applied by immersion, and exposure to steam in a pasteurization system, in a cabinet, or in combination with vacuum were evaluated for their effectiveness in reducing levels of bacterial contamination on samples of beef cheek meat, large intestine, lips, liver, oxtail, and tongue. treated samples (five per treatment) and controls were analyzed for aerobic plate counts ( ... | 2000 | 10643768 |
| use of 8-hydroxyquinoline-beta-d-glucuronide for presumptive identification of shiga toxin-producing escherichia coli o157. | 8-hydroxyquinoline-beta-d-glucuronide (hqg) was used to improve the presumptive identification of shiga toxin-producing escherichia coli o157 (stec o157) on sorbitol macconkey agars (smac). advantages of hqg are (i) that it is less expensive than 5-bromo-4-chloro-3-indoxyl-glucuronide; (ii) that it is visible in normal daylight and (iii) that it does not diffuse into the agar like 4-methylumbelliferryl-beta-d-glucuronide (mug). sixteen stec o157 isolates, 91 bovine mastitis-associated e. coli is ... | 2000 | 10792673 |
| biosynthesis of heparin/heparan sulphate: mechanism of epimerization of glucuronyl c-5. | in the biosynthesis of heparin and heparan sulphate, d-glucuronic acid residues are converted into l-iduronic acid (idoa) units by c-5 epimerization, at the polymer level. the reaction catalysed by the epimerase occurs by reversible abstraction and readdition of a proton at c-5 of target hexuronic acid residues, through a carbanion intermediate, with or without an inversion of configuration at c-5 [prihar, campbell, feingold, jacobsson, jensen, lindahl and rodén (1980) biochemistry 19, 495-500]. ... | 2000 | 10727403 |
| isolation, characterization and expression of a gra2 homologue from neospora caninum. | a cdna library derived from mrna of tachyzoites of neospora caninum (nc-liverpool strain) was screened with antisera from a cow naturally infected with n. caninum. the dna sequence of 1 recombinant isolated predicted a significant protein sequence homology of the gene product to the 28 kda (gra2) antigen of toxoplasma gondii. studies on the n. caninum gene coding for this antigen demonstrated the presence of a single intron flanked by 2 exons; the gene was also highly expressed in culture-derive ... | 2000 | 10811279 |
| comparison of membrane filtration rates and hydrophobic grid membrane filter coliform and escherichia coli counts in food suspensions using paddle-type and pulsifier sample preparation procedures. | food suspensions prepared by pulsifier contained less debris and filtered 1.3x to 12x faster through hydrophobic grid membrane filters (hgmfs) than those prepared by stomacher 400. coliform and escherichia coli counts made by an hgmf method yielded 84 and 36 paired samples, respectively, positive by both suspending methods. overall counts of pulsificates and stomachates did not differ significantly for either analysis, though coliform counts by pulsifier were significantly higher in mushrooms an ... | 2000 | 10643783 |
| brucella abortus strain rb51 as a vector for heterologous protein expression and induction of specific th1 type immune responses. | brucella abortus strain rb51 is a stable, rough, attenuated mutant widely used as a live vaccine for bovine brucellosis. our ultimate goal is to develop strain rb51 as a preferential vector for the delivery of protective antigens of other intracellular pathogens to which the induction of a strong th1 type of immune response is needed for effective protection. as a first step in that direction, we studied the expression of a foreign reporter protein, beta-galactosidase of escherichia coli, and th ... | 2000 | 10816476 |
| replacement of histidine 340 with alanine inactivates the group a streptococcus extracellular cysteine protease virulence factor. | streptococcus pyogenes expresses a highly conserved extracellular cysteine protease that is a virulence factor for invasive disease, including soft tissue infection. site-directed mutagenesis was used to generate a his340ala recombinant mutant protein that was made as a stable 40-kda zymogen by escherichia coli. purified his340ala protein was proteolytically inactive when bovine casein and human fibronectin were used as substrates. wild-type 28-kda streptococcal protease purified from s. pyogene ... | 2000 | 10816533 |
| subtypes of intimin among non-toxigenic escherichia coli from diarrheic calves in brazil. | one hundred and five strains of escherichia coli that were isolated from calves with diarrhea in the state of são paulo, brazil, and were negative for enterotoxins and cytotoxins, were examined for the eae gene. four (3.8%) strains were positive by polymerase chain reaction (pcr) and were shown to produce intimin by using western blot with specific antiserum against the conserved n-terminal region of intimin. subtyping of the intimins was done by pcr with specific primers and by western blot wit ... | 2000 | 10680651 |
| inhibition of protein phosphatase-1 by clavosines a and b. novel members of the calyculin family of toxins. | site-directed mutagenesis was used to investigate the mechanism of interaction between the catalytic subunit of human protein phosphatase-1 (pp-1cgamma) and members of the calyculin family of toxins. clavosines a and b are related to calyculins but are glycosylated with a trimethoxy rhamnose group. we provide experimental evidence implicating tyr-134 as an important residue in pp-1cgamma that mediates interactions with the calyculins. mutation of tyr-134 to phe, to prevent hydrogen bond formatio ... | 2000 | 10660582 |
| clonal turnover of enterohemorrhagic escherichia coli o157:h7 in experimentally infected cattle. | a total of 401 enterohemorrhagic escherichia coli (ehec) o157:h7 isolates from two experimentally infected calves were analyzed using molecular biological methods. genetic differences detected by pulsed-field gel electrophoresis were observed between the inoculated and recovered strains as early as 1 day post inoculation. the loss of the inoculated clone was observed in one calf. replication and dissemination of the ehec o157:h7 strains that mutated in cattle may result in the diversification of ... | 2000 | 10689170 |
| purification and characterization of chitin-binding proteins from the hemolymph of sweet potato hornworm, agrius convolvuli. | three chitin-binding proteins (cbps: cbp9, cbp15, cbp66) were identified from the larval hemolymph of sweet potato hornworm, agrius convolvuli. two (cbp9 and cbp15) of them have been isolated and purified by gel filtration (superdex hr 75), cation-exchange chromatography (mono s), and reverse-phase chromatography (murpc pc 2.1/3). in experiments to detect cbps in hemolymph, we examined whether ionic strength and existence of bovine serum albumin in the incubation solution influenced binding affi ... | 1999 | 10665375 |
| some emerging food and water borne pathogens. | emerging pathogens are those infective organisms whose incidence has recently increased or is likely to increase during the next two decades due to changes in demography, food habits, food technology, commerce, water sources and environmental factors. some important emerging food and water borne bacterial pathogens include listeria monocytogenes, campylobacter jejuni, yersinia enterocolitica, salmonella enteritidis, escherichia coli o157: h7, vibrio cholerae biotype e1 tor serotype 0139, vibrio ... | 1999 | 10810592 |
| the effects of the surface charge and hydrophobicity of escherichia coli on its adhesion to beef muscle. | the surface characteristics of escherichia coli strains were studied to evaluate the effect upon bacterial adhesion to beef muscle. the influence of suspension conditions upon the surface charge of a pathogenic strain, e. coli o157:h7 (ec01), and a saprophytic laboratory strain, e. coli jm109 (ec22) were investigated and compared. the cellular surface charge of most e. coli o157:h7 strains were much less affected by changes in the ph, ionic strength or concentration of surfactants in the suspend ... | 1999 | 10634709 |
| phosphoserine aminotransferase, the second step-catalyzing enzyme for serine biosynthesis. | as a step toward analyzing the serine biosynthetic pathway in mammals, we have studied the properties of phosphoserine aminotransferase, the second step-catalyzing enzyme. the k(m) values for 3-phosphohydroxypyruvate and l-phosphoserine are 5 and 35 microm, respectively, and those for glutamate and alpha-ketoglutarate are 1.2 and 0.8 mm, respectively. the product inhibition studies strengthened the support for a ping-pong mechanism and allowed evaluation of ki values for the four substrates. the ... | 1999 | 10637769 |
| protective cultures inhibit growth of listeria monocytogenes and escherichia coli o157:h7 in cooked, sliced, vacuum- and gas-packaged meat. | contamination of cooked meat products with listeria monocytogenes poses a constant threat to the meat industry. the aim of this study was therefore to investigate the use of indigenous lactic acid bacteria (lab) as protective cultures in cooked meat products. cooked, sliced, vacuum- or gas-packaged ham and servelat sausage from nine meat factories in norway were inoculated with 10(3) cfu/g of a mixture of three rifampicin resistant (rif-mutant) strains of l. monocytogenes and stored at 8 degrees ... | 1999 | 10598113 |
| crystal structure of the purine nucleoside phosphorylase (pnp) from cellulomonas sp. and its implication for the mechanism of trimeric pnps. | the three-dimensional structure of the trimeric purine nucleoside phosphorylase (pnp) from cellulomonas sp. has been determined by x-ray crystallography. the binary complex of the enzyme with orthophosphate was crystallized in the orthorhombic space group p212121 with unit cell dimensions a=64.1 a, b=108.9 a, c=119.3 a and an enzymatically active trimer in the asymmetric unit. x-ray data were collected at 4 degrees c using synchrotron radiation (embl/desy, hamburg). the structure was solved by m ... | 1999 | 10600382 |
| modulation of the remote heme site geometry of recombinant mouse neuronal nitric-oxide synthase by the n-terminal hook region. | the role of two essential residues at the n-terminal hook region of neuronal nitric-oxide synthase (nnos) in nitric-oxide synthase activity was investigated. full-length mouse nnos proteins containing single-point mutations of thr-315 and asp-314 to alanine were produced in the escherichia coli and baculovirus-insect cell expression systems. the molecular properties of the mutant proteins were analyzed in detail by biochemical, optical, and electron paramagnetic resonance spectroscopic technique ... | 1999 | 10075660 |
| role of lactosyl glycan sequences in inhibiting enteropathogenic escherichia coli attachment. | previously, we found that asialo-lactosamine sequences served as receptors for enteropathogenic escherichia coli (epec) binding to chinese hamster ovary (cho) cells. in the present report, we have extended these earlier results by examining the ability of lactosamine- or fucosylated lactosamine-bovine serum albumin (bsa) glycoconjugates to inhibit epec, strain e2348/69, binding to hep-2 cells. we found that, consistent with our previous findings with cho cells, n-acetyllactosamine-bsa was the mo ... | 1999 | 10377105 |
| expression of bovine cytokines in escherichia coli. | glutathione s-transferase fusion proteins of bovine interleukin-2 (il-2), il-4, il-6 and interferon-gamma (ifn-gamma) were expressed in escherichia coli. complementary dna (cdna) for open reading frame of each cytokine without signal peptide encoding region was amplified by reverse transcriptional polymerase chain reaction method and was subcloned into pgex-5x-1. in result, il-6 and ifn-gamma fusion proteins in bacteria were soluble, but il-2 and il-4 fusion proteins were insoluble. the insolubl ... | 1999 | 10081758 |
| unusual sites of arginine methylation in poly(a)-binding protein ii and in vitro methylation by protein arginine methyltransferases prmt1 and prmt3. | arginine methylation is a post-translational modification found mostly in rna-binding proteins. poly(a)-binding protein ii from calf thymus was shown by mass spectrometry and sequencing to contain ng, ng-dimethylarginine at 13 positions in its amino acid sequence. two additional arginine residues were partially methylated. almost all of the modified residues were found in arg-xaa-arg clusters in the c terminus of the protein. these motifs are distinct from arg-gly-gly motifs that have been previ ... | 1999 | 10224081 |
| effect of cattle diet on escherichia coli o157:h7 acid resistance. | the duration of shedding of escherichia coli o157 isolates by hay-fed and grain-fed steers experimentally inoculated with e. coli o157:h7 was compared, as well as the acid resistance of the bacteria. the hay-fed animals shed e. coli o157 longer than the grain-fed animals, and irrespective of diet, these bacteria were equally acid resistant. feeding cattle hay may increase human infections with e. coli o157:h7. | 1999 | 10388727 |
| characterization of the cardiac holotroponin complex reconstituted from native cardiac troponin t and recombinant i and c. | cardiac troponin i (ctni), the inhibitory subunit of cardiac troponin (ctn), is phosphorylated by the camp-dependent protein kinase a at two adjacently located serine residues within the heart-specific n-terminal elongation. four different phosphorylation states can be formed. to investigate each monophosphorylated form ctni mutants, in which each of the two serine residues is replaced by an alanine, were generated. these mutants, as well as the wild-type cardiac troponin i (ctni-wt) have been e ... | 1999 | 10103031 |
| real-time monitoring of escherichia coli o157:h7 adherence to beef carcass surface tissues with a bioluminescent reporter. | a method for studying bacteria that are attached to carcass surfaces would eliminate the need for exogenous sampling and would facilitate understanding the interaction of potential human food-borne pathogens with food animal tissue surfaces. we describe such a method in which we used a bioluminescent reporter strain of escherichia coli o157:h7 that was constructed by transformation with plasmid pcgls1, an expression vector that contains a complete bacterial luciferase (lux) operon. beef carcass ... | 1999 | 10103275 |
| catalase hpii from escherichia coli exhibits enhanced resistance to denaturation. | catalase hpii from escherichia coli is a homotetramer of 753 residue subunits. the multimer displays a number of unusual structural features, including interwoven subunits and a covalent bond between tyr415 and his392, that would contribute to its rigidity and stability. as the temperature of a solution of hpii in 50 mm potassium phosphate buffer (ph 7) is raised from 50 to 92 degrees c, the enzyme begins to lose activity at 78 degrees c and 50% inactivation has occurred at 83 degrees c. the ina ... | 1999 | 10194300 |
| expression of recombinant bovine l-selectin in escherichia coli and insect cells. | bovine l-selectin was expressed in bacteria using pgex vector and in insect cells infected with recombinant baculovirus in order to obtain recombinant protein for preparation of specific antiserum and its functional studies. in bacterial expression, l-selectin fusion protein with glutathione s-transferase was detected in the insoluble fraction with the expected molecular weight of 60 kda by sds-page and reacted with anti-bovine cd62l monoclonal antibody in immunoblot analysis. in insect cells in ... | 1999 | 10331197 |
| effect of antibiotic induced bacterial clearance in the udder on l-selectin shedding of blood neutrophils in cows with escherichia coli mastitis. | bacterial clearance, l-selectin adhesion receptor expression and neutrophil recruitment during experimentally induced escherichia coli mastitis were investigated. acute mastitis was induced by injection of 10(4) cfu e. coli into both left quarters of 12 clinically healthy lactating cows, 2-6 weeks after parturition. half the cows were treated intravenously 10 h after infection, and subcutaneously 30 h after infection with enrofloxacin. in both groups, bacterial count, milk serum albumin, milk so ... | 1999 | 10206204 |
| molecular cloning of a novel human collectin from liver (cl-l1). | collectins are a c-lectin family with collagen-like sequences and carbohydrate recognition domains. these proteins can bind to carbohydrate antigens of microorganisms and inhibit their infection by direct neutralization and agglutination, the activation of complement through the lectin pathway, and opsonization by collectin receptors. here we report the cloning of a cdna encoding human collectin from liver (cl-l1 (collectin liver 1)) that has typical collectin structural characteristics, consist ... | 1999 | 10224141 |
| excretion of vtec o157 by cattle. | 1999 | 10420488 | |
| expression of inducible nitric oxide synthase in spontaneous bovine bronchopneumonia. | the expression of inducible nitric oxide synthase (inos), major histocompatibility class ii molecules (mhc-ii), cd68, and the calcium-binding proteins s100a8 and s100a9 (also called mrp8 and mrp14, respectively) was assessed in lung tissues from cattle that succumbed to pneumonia. expression patterns of these markers were related to the types of lung lesion. inos expression was only observed in lungs infected with arcanobacterium pyogenes or pasteurella haemolytica but not in lungs from cattle w ... | 1999 | 10490207 |
| expression, purification, and crystal structure determination of recombinant human epidermal-type fatty acid binding protein. | we describe the crystal structure of human epidermal-type fatty acid binding protein (e-fabp) that was recently found to be highly upregulated in human psoriatic keratinocytes. to characterize e-fabp with respect to ligand-binding properties and tertiary structure, we cloned the respective cdna, overexpressed the protein in escherichia coli and purified it to homogeneity by a combination of ion-exchange and size-exclusion chromatographic steps with a yield of 30 mg/l broth. the purified protein ... | 1999 | 10493790 |
| production of engineered human pancreatic ribonucleases, solving expression and purification problems, and enhancing thermostability. | human pancreatic ribonuclease, the homolog of bovine pancreatic ribonuclease, has a significant therapeutic potential. its study has been hindered by the difficulty of obtaining the enzyme in a pure and homogeneous form, either from human source or using heterologous expression. engineering of different variants of human pancreatic ribonuclease has allowed us to study and overcome some problems encountered during its heterologous production in an escherichia coli system and its purification from ... | 1999 | 10497083 |
| micellar electrokinetic chromatography as a complementary method to sodium dodecyl sulfate-polyacrylamide gel electrophoresis for studying limited proteolysis of proteins. | micellar electrokinetic chromatography (mekc) has been utilized as an analytical method to perform investigations on limited proteolysis of proteins. to this purpose partial proteolysis experiments with a series of proteinases were performed, utilizing as model protein pyruvate kinase (pk) from escherichia coli, an enzyme that is regulated allosterically by fructose 1,6-bisphosphate (fbp). data obtained with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and mekc were compa ... | 1999 | 10499331 |
| detection of low dose radiation induced dna damage using temperature differential fluorescence assay. | a rapid and sensitive fluorescence assay for radiation-induced dna damage is reported. changes in temperature-induced strand separation in both calf thymus dna and plasmid dna (puc 19 plasmid from escherichia coli) were measured after exposure to low doses of radiation. exposures of between 0.004 and 1 gy were measured with doses as low as 0.008 gy yielding significant responses. the double-strand, sensitive dye picogreen was used as an indicator of dna denaturation. calibration plots indicate t ... | 1999 | 10517153 |
| polycitone a, a novel and potent general inhibitor of retroviral reverse transcriptases and cellular dna polymerases. | polycitone a, an aromatic alkaloid isolated from the ascidian polycitor sp. exhibits potent inhibitory capacity of both rna- and dna-directed dna polymerases. the drug inhibits retroviral reverse transcriptase (rt) [i.e. of human immunodeficiency virus type 1 (hiv), murine leukaemia virus (mlv) and mouse mammary tumour virus (mmtv)] as efficiently as cellular dna polymerases (i.e. of both dna polymerases alpha and beta and escherichia coli dna polymerase i). the mode and mechanism of inhibition ... | 1999 | 10548537 |
| rapid and sensitive detection of escherichia coli o157:h7 in bovine faeces by a multiplex pcr. | cattle are considered the major reservoir for escherichia coli o157:h7, one of the newly emerged foodborne human pathogens of animal origin and a leading cause of haemorrhagic colitis in humans. a sensitive test that can accurately and rapidly detect the organism in the food animal production environment is critically needed to monitor the emergence, transmission, and colonization of this pathogen in the animal reservoir. in this study, a novel multiplex polymerase chain reaction (pcr) assay was ... | 1999 | 10664910 |
| [fecal shedding and serum antibody responses of cattle experimentally infected with enterohaemorrhagic escherichia coli o157:h7]. | 1999 | 10565127 | |
| occurrence of escherichia coli o157:h7 in hamburgers produced in brazil. | the occurrence of escherichia coli o157:h7 was evaluated in 886 samples of hamburgers produced by eight manufacturers in the south and southeast of brazil from january to september of 1997. the pathogen was not detected in any of the samples analyzed, although 17 (1.9%) showed the presence of e. coli strains, which presented agglutination with o157 antiserum. these strains were not confirmed, since a subsequent identification indicated that they were fermentation positive for sorbitol, incapable ... | 1999 | 10571325 |
| 1,25-dihydroxyvitamin d3 enhances milk antibody titers to escherichia coli j5 vaccine. | recent evidence in cattle and mice has suggested that 1,25-dihydroxyvitamin d3 may stimulate t-lymphocyte differentiation pathways responsible for humoral immunity. the use of 1,25-dihydroxyvitamin d3 as an adjuvant for an escherichia coli j5 vaccine was tested. ten midlactation cows received j5 vaccine and were revaccinated 6 wk after the first vaccine administration. five of these cows were given 200 micrograms of 1,25-dihydroxyvitamin d3, in addition to the primary vaccination, and they recei ... | 1999 | 10509248 |
| prevalence and infection risks of zoonotic enteropathogenic bacteria in swiss cow-calf farms. | a longitudinal study was performed in 67 larger swiss cow-calf farms from september 1996 through november 1997. the objectives of the study were to estimate prevalence and risk factors for colonization with potentially zoonotic enteropathogenic bacteria in younger calves and in calves at weaning age. the study included data from 395 calves with three to four fecal samples each. fecal samples were analyzed for campylobacter spp., verotoxin producing e. coli (vtec), yersinia spp. and salmonella sp ... | 1999 | 10535771 |
| investigating site-specific effects of the -x glutamate in a parvalbumin cd site model peptide. | the -x glutamate in a 33-residue model peptide comprising the cd site of carp parvalbumin 4.25 (parvcd) was replaced with aspartate (parvcd-xd) and the effect on calcium-dependent dimerization and calcium affinity assessed. the peptide parvcd demonstrates a 10(5)-fold lower calcium affinity than the same site in the native protein. both the parvcd and parvcd-xd model peptides fail to bind magnesium. the low calcium affinity and failure of the model parvcd site to bind magnesium may be due to hig ... | 1999 | 10562419 |
| genotoxic effects of benzyl isothiocyanate, a natural chemopreventive agent. | benzyl isothiocyanate (bitc) is contained in cruciferous plants which are part of the human diet. numerous reports indicate that bitc prevents chemically induced cancer in laboratory animals and it has been postulated that bitc might also be chemoprotective in humans. on the other hand, evidence is accumulating that this compound is a potent genotoxin in mammalian cells by itself. to further elucidate the potential hazards of bitc, we investigated its genotoxic effects in different in vitro geno ... | 1999 | 10567035 |
| the effect of alpha-lactalbumin and beta-lactoglobulin hydrolysates on the metabolic activity of escherichia coli jm103. | bovine milk proteins alpha-lactalbumin (alpha-la) and beta-lactoglobulin (beta-lg) were hydrolysed with seven different proteolytic enzymes, and the effect of various hydrolysates on a genetically modified luminous escherichia coli jm103 was tested in vitro with a bioluminescence assay for bacterial growth and metabolism. undigested proteins did not inhibit the activity of tested e. coli jm103 at a concentration as high as 0.1 g ml-1. at the same concentrations, alpha-la hydrolysed with pepsin o ... | 1999 | 10583682 |
| agar underlay method for recovery of sublethally heat-injured bacteria. | a method of recovering sublethally heat-injured bacteria was developed. the procedure (termed the agar underlay method) uses a nonselective agar underlaid with a selective medium. in a two-chambered petri dish, the lutri plate (lp), a nonselective agar is inoculated with a population of sublethally heat-injured bacteria. after a 2-h repair incubation period, selective agar is added to the bottom chamber of the lp and incubated. by diffusing through the nonselective top agar, selective agents fro ... | 1999 | 10583985 |
| unusual amino acid usage in the variable regions of mercury-binding antibodies. | monoclonal antibodies (mab) specific for mercuric ions were isolated from balb/c mice injected with a mercury-containing, hapten-carrier complex. the antibodies reacted by enzyme-linked immunosorbent assay with bovine serum albumin-glutathione-mercuric chloride (bsa-gsh-hgcl) but not with bsa-gsh without mercury. nucleotide sequences from polymerase chain reaction products encoding six of the antibody heavy-chain variable regions and seven light-chain variable regions revealed that all the antib ... | 1999 | 10591102 |
| use of heme-protein complexes by the yersinia enterocolitica hemr receptor: histidine residues are essential for receptor function. | the abilities of two bacterial active heme transporters, hmbr of neisseria meningitidis and hemr of yersinia enterocolitica, to use different heme sources were compared. while hmbr-expressing cells used only hemoglobin (hb) and heme, hemr-expressing bacteria were able to grow on hb, heme, myoglobin, hemopexin, catalase, human and bovine serum albumin-heme, and haptoglobin-hemoglobin complexes as sources of iron. expression of functional hemr allowed escherichia coli cells to respond to heme-cont ... | 1999 | 10498719 |
| experimental infection of calves with escherichia coli o157:h7. | three 3-month-old japanese black calves were experimentally infected with escherichia coli o157:h7 to define the magnitude (cfu/g) and duration of fecal shedding of the organism. in two of the three calves, fecal shedding of e. coli o157:h7 ceased in 5 and 9 weeks. the remaining calf continued shedding e. coli o157:h7 for more than 31 weeks, and the magnitude of the shedding ranged from 10(1) to 10(4) cfu/g of feces. the possibility is suggested that a percentage of animals naturally infected wi ... | 1999 | 10563303 |
| comparative sensitivity of immunoassays for haptens using monomeric and dimeric antibody fragments. | a single-chain anti-atrazine antibody fragment, scab (single-chain fv with a ck domain), was expressed in escherichia coli, and monomeric and dimeric species were preferentially purified from periplasmic extracts by chromatography upon nickel chelate immunosorbent columns or by immunoaffinity purification using a constant domain (ck) tag. recombinant monomeric and dimeric antibody fragments, fab, and intact monoclonal antibodies were compared in assays by competition between free atrazine in sol ... | 1999 | 10563896 |
| high occurrence of shiga toxin-producing escherichia coli (stec) in healthy cattle in rio de janeiro state, brazil. | in order to evaluate the prevalence of shiga toxin-producing escherichia coli (stec) strains, 197 fecal samples of healthy cattle from 10 dairy farms, four beef farms and one slaughterhouse at rio de janeiro state, brazil, were examined for shiga toxin (stx) gene sequences by polymerase chain reaction (pcr). for presumptive isolation of o157:h7 e. coli, the cefixime-potassium tellurite-sorbitol macconkey agar (ct-smac) was used. a high occurrence (71%) of stx was detected, and was more frequentl ... | 1999 | 10591502 |
| characterization of two fungal-elicitor-induced rice cdnas encoding functional homologues of the rab-specific gdp-dissociation inhibitor. | by using the mrna differential display approach to isolate defense signaling genes active at the early stage of fungal infection two cdna fragments with high sequence homology to rab-specific gdp-dissociation inhibitors (gdis) were identified in rice (oryza sativa l.) suspension cells. using polymerase-chain-reaction products as probes, two full-length cdna clones were isolated from a cdna library of fungal-elicitor-treated rice, and designated as osgdi1 and osgdi2. the deduced amino acid sequen ... | 1999 | 10592042 |
| escherichia coli o157:h7 in retail ground beef in seattle: results of a one-year prospective study. | escherichia coli o157:h7 was sought systematically in 1,400 samples of retail ground beef in seattle in a 1-year prospective study. sorbitol-nonfermenting, lactose-fermenting, indole-positive colonies isolated after enrichment culture were probed for the presence of shiga toxin genes. totals of 67,040 sorbitol-nonfermenting and 66,705 sorbitol-fermenting colonies were characterized, but e. coli o157:h7 was not identified. the sensitivity of this technique was usually sufficient to detect e. coli ... | 1999 | 10030631 |
| interactions of the papovavirus dna replication initiator proteins, bovine papillomavirus type 1 e1 and simian virus 40 large t antigen, with human replication protein a. | papovaviruses utilize predominantly cellular dna replication proteins to replicate their own viral genomes. to appropriate the cellular dna replication machinery, simian virus 40 (sv40) large t antigen (tag) binds to three different cellular replication proteins, the dna polymerase alpha-primase complex, the replication protein a (rpa) complex, and topoisomerase i. the functionally similar papillomavirus e1 protein has also been shown to bind to the dna polymerase alpha-primase complex. enzyme-l ... | 1999 | 10233951 |
| cytochrome p450c17-expressing escherichia coli as a first-step screening system for 17alpha-hydroxylase-c17,20-lyase inhibitors. | we have designed and synthesized a number of cytochrome p450 17alpha-hydroxylase-c17,20-lyase (p450c17) inhibitors with the aim of inhibiting androgen synthesis. to select the most potent inhibitors, we initially used human testicular microsomes, which have a high level of expression of this enzyme. however, due to lack of availability of human tissue and variability among the samples, we utilized recombinant human enzyme expressed in escherichia coli. we designed a simple and economical protoco ... | 1999 | 10036137 |
| evaluation of the role of endotoxin and cortisol on modulation of cd18 adhesion receptors in cows with mastitis caused by escherichia coli. | to determine the effect of mastitis caused by escherichia coli on expression of cd18 cell surface receptors and to evaluate the involvement and regulation of receptors by lipopolysaccharide (lps) and cortisol. | 1999 | 10328420 |
| assessment of the adequacy of cleaning of equipment used for breaking beef carcasses. | enumeration of bacteria on product entering or leaving a beef carcass breaking process showed that the meat was being contaminated with escherichia coli during the process. the equipment used in the process appeared to be well cleaned, and few bacteria were recovered from meat-contacting surfaces of cleaned equipment. however, careful inspection of the cleaned equipment revealed obscured locations in the equipment which harboured detritus that carried large numbers of aerobic bacteria including ... | 1999 | 10050680 |
| collaborative evaluation of detection methods for escherichia coli o157:h7 from radish sprouts and ground beef. | for the evaluation of plating and immunological methods applicable to the detection of escherichia coli o157:h7 from ground beef and radish sprouts, a collaborative study was conducted. it focused on a comparison of the efficiency of the plating and immunological methods using various plating agars and immuno-kits in combination with enrichment in modified e. coli broth supplemented with novobiocin (mec + n), and using immunomagnetic separation. the plating media tested were sorbitol macconkey a ... | 1999 | 10050682 |
| characterization of rpos alleles in escherichia coli o157:h7 and in other e. coli serotypes. | the rpos nucleotide and predicted amino acid sequences from three escherichia coli o157:h7 isolates were compared with those from three other e. coli isolates, including the likely o157:h7 progenitor, e. coli o55:h7. these clinical and environmental isolates all had identical sigma s amino acid sequences, while laboratory strains k12 and dh1 had three and one amino acid alterations, respectively, in comparison with the majority sequence. to extend the analysis of sigma s sequence conservation to ... | 1999 | 10063629 |
| genomic dna restriction site heterogeneity in bovine pasteurella multocida serogroup a isolates detected with an rrna probe. | a total of 81 pasteurella multocida isolates from healthy and diseased dairy and beef cattle originating from various geographical locations was examined by rrna gene restriction site polymorphism analysis (ribotyping), restriction endonuclease analysis (rea), sds-page analysis of whole-cell (wcp) and outer-membrane (omp) proteins, and capsule and somatic serotyping. bacterial strains were isolated from nose, lung and in one case testicle, of holstein and cross-bred beef cattle. the isolates rep ... | 1999 | 10334595 |
| new general approach for determining the solution structure of a ligand bound weakly to a receptor: structure of a fibrinogen aalpha-like peptide bound to thrombin (s195a) obtained using noe distance constraints and an ecepp/3 flexible docking program. | a new approach incorporating flexible docking simulations and nmr data is presented for calculating the bound conformation of a ligand that interacts weakly with an enzyme. this approach consists of sampling directly the conformation of a flexible ligand inside a receptor active site containing surrounding flexible loops. to make this sampling efficient, a ligand-growing procedure has been adopted. optimization of the ecepp/3-plus-noe constraint function is carried out by using a collective vari ... | 1999 | 10336381 |
| detection of escherichia coli o157:h7 from musca domestica (diptera: muscidae) at a cattle farm in japan. | enterohemorrhagic escherichia coli (ehec) o157:h7 was isolated for the first time from musca domestica l. a total of 310 fly samples was collected from 4 different farms in obihiro-city, hokkaido, in the summer and autumn of 1997;5 samples carried e. coli serotype o157:h7. using elisa and vero cell cytotoxicity assay, 3 isolates from 1 cattle farm produced both active shiga-toxin type 1 (stx1) and 2 (stx2). these isolates also carried hemolysin and eaea genes and harbored the 90-kb virulence pla ... | 1999 | 10071501 |
| pattern of diarrhoea in newborn beef calves in south-west france. | a prospective study was carried out on 94 randomly selected beef herds in the midi-pyrénées region in france. the objective was to describe diarrhoea and mortality in beef calves from birth to 30 days of age. calves (3,080) were followed from december 1995 to april 1996, and a total of 700 visits allowed records of herd management practices, individual data and environmental conditions to be collected. the incidence rate for diarrhoea during the neonatal period was 14.6%, and varied markedly bet ... | 1999 | 10081113 |
| influence of oxygen availability on physiology, verocytotoxin expression and adherence of escherichia coli o157. | a strain of escherichia coli serotype o157 was grown in steady state chemostat culture under aerobic, oxygen-limited and anaerobic conditions. the growth and metabolic efficiency of oxygen-limited and anaerobic cultures was impaired, with biomass yield and the molar growth yield for glucose, yglucose, reduced markedly in comparison with aerobic cultures. steady state cells were typically short rods 2-3 microns long, and were encapsulated by a layer of extracellular material. the majority of cell ... | 1999 | 10030015 |
| a recombinant bovine gallbladder mucin polypeptide binds biliary lipids and accelerates cholesterol crystal appearance time. | mucin has a central role in the pathogenesis of cholesterol gallstones, in part because of its ability to bind biliary lipids and accelerate cholesterol crystal appearance time. previous studies have localized these properties to nonglycosylated mucin domains, and we have recently shown that these domains contain a series of 127-amino acid, cysteine-rich repeats. the aim of this study was to express a recombinant mucin polypeptide containing these repeats and investigate its lipid-binding and pr ... | 1999 | 10092316 |
| chloroplast nadh dehydrogenase from pisum sativum: characterization of its activity and cloning of ndhk gene. | the pea chloroplast ndhk gene coding for a component of a nadh-plastoquinone oxidoreductase has been cloned and sequenced. this gene codes for a polypeptide of 227 amino acids and a predicted molecular mass of 25,495 da which belongs to the family of the 20 kda psst subunit of the bovine mitochondrial complex i. a fragment of this gene has been overexpressed in escherichia coli, and antibodies against the expressed polypeptide recognize a protein of the predicted molecular mass from pea thylakoi ... | 1999 | 10202810 |
| effects of ph and acid resistance on the radiation resistance of enterohemorrhagic escherichia coli. | the effects of ph and the induction of ph-dependent stationary-phase acid resistance on the radiation resistance of escherichia coli were determined for seven enterohemorrhagic strains and one nonenterohemorrhagic strain. the isolates were grown in acidogenic or nonacidogenic media to ph levels of approximately 4.7 and 7.2, respectively. the cells were then transferred to brain heart infusion (bhi) broth adjusted to ph 4.0, 4.5, 5.0, and 5.5 (with hcl) that was preequilibrated to 2 degrees c, an ... | 1999 | 10090239 |
| vaccines and diagnostic methods for bovine mastitis: fact and fiction. | a number of problems are uniquely associated with vaccination of dairy cows for mastitis. one of these is that the number of mastitis pathogens is numerous and heterogeneous. vaccine efforts have concentrated mainly on the major mastitis pathogens. while at least one s. aureus bacterin has been commercially available for a number of years, no large-scale, independent field trials have been published in refereed journals which support the efficacy of this vaccine. experimental vaccines for s. aur ... | 1999 | 9890021 |
| roles of pilc and pile proteins in pilus-mediated adherence of neisseria gonorrhoeae and neisseria meningitidis to human erythrocytes and endothelial and epithelial cells. | unlike other type 4 pili, the neisserial pili consist of at least two distinct proteins, the highly variable major subunit pile forming the pilus fiber and the tip-associated adhesin pilc. pilc protein purified either from gonococci or from escherichia coli interacted with different human epithelial cell lines, primary epithelial and endothelial cells. the binding of pilc protein efficiently prevented the attachment of piliated neisseria gonorrhoeae and neisseria meningitidis to these cell types ... | 1999 | 9916098 |
| lipopolysaccharide enhances the production of vascular endothelial growth factor by human pulp cells in culture. | we investigated whether vascular endothelial growth factor (vegf) production by human pulp cells (hpc) is regulated by lipopolysaccharide (lps) in relation to the pathogenesis of pulpitis. although hpc incubated with medium alone only marginally expressed vegf mrna and produced a low level of vegf as detected by enzyme-linked immunosorbent assay, the vegf mrna expression and vegf production were markedly enhanced upon stimulation with lps from escherichia coli. prevotella intermedia lps, phorbol ... | 1999 | 10084996 |
| cryptosporidium and concurrent infections with other major enterophatogens in 1 to 30-day-old diarrheic dairy calves in central spain. | faeces samples from 218, 1 to 30-day-old, diarrheic dairy calves in 65 dairy herds were screened for the presence of cryptosporidium and concurrent infections with rotavirus, coronavirus, f5 escherichia coli and salmonella spp. calves were grouped according to their age as follows: 1-7, 8-14, 15-21 and 22-30 days. cryptosporidium infection was detected in 43.8%, 71.9%, 63.2% and 6.9% of the calves in the respective age groups. significant differences in the detection rate of cryptosporidium were ... | 1999 | 9950342 |
| detection of multidrug-resistant salmonella enterica serotype typhimurium dt104 based on a gene which confers cross-resistance to florfenicol and chloramphenicol. | salmonella enterica serotype typhimurium (s. typhimurium) dt104 (dt104) first emerged as a major pathogen in europe and is characterized by its pentadrug-resistant pattern. it has also been associated with outbreaks in the united states. the organism typically carries resistance to ampicillin, chloramphenicol, streptomycin, sulfonamides, and tetracycline. the mechanism of chloramphenicol resistance in dt104 was determined by producing antibiotic-resistant escherichia coli host strain clones from ... | 1999 | 10203484 |
| recurrent clinical mastitis caused by escherichia coli in dairy cows. | in this study, the occurrence of persistent intramammary infections caused by escherichia coli with recurrent episodes of clinical mastitis caused by e. coli are described for a cohort of 300 dutch dairy herds. calculations on the recurrent episodes were based on data collected by dairy farmers. the genotype of the e. coli strains was determined by means of a polymerase chain reaction using enterobacterial repetitive intergenic consensus (eric) primers, resulting in a dna fingerprint. quarters i ... | 1999 | 10022009 |
| antibacterial activity of gentamicin and ciprofloxacin against gram-negative bacteria: interactions with pig and calf sera. | the antibacterial activity of pig and calf serum and its ability to interact with gentamicin and ciprofloxacin were studied in vitro using escherichia coli k-12, proteus rettgeri (sanelli) and klebsiella pneumoniae atcc 10031. the antimicrobial activity of the above drugs, alone or in combination with serum, was investigated by the checkerboard method and expressed as the minimal inhibitory concentration (microg ml-1). pig serum (25%) with gentamicin had a synergistic antibacterial effect agains ... | 1999 | 10208763 |
| transcriptional inhibition of the operon for the spermidine uptake system by the substrate-binding protein potd. | inhibition of spermidine uptake in escherichia coli, which occurs in the presence of accumulated polyamines, has been studied using the spermidine uptake operon consisting of the pota, -b, -c, and -d genes. transcription of the potabcd operon was inhibited by potd, a spermidine-binding protein usually found in the periplasm, and the inhibitory effect of potd was increased by spermidine. transcription was not affected by bovine serum albumin, pota, or potf, suggesting that the effects of potd are ... | 1999 | 9890949 |
| role of carboxyl-terminal charges on s-modulin membrane affinity and inhibition of rhodopsin phosphorylation. | s-modulin shows a higher affinity for urea-stripped frog rod outer segment membranes than s26 (a cone homologue of s-modulin). nacl at a concentration of several hundred millimolar reduced the membrane affinity of s-modulin to the s26 level. chimeric s-modulin and s26 whose respective 23 and 29 amino acids at the carboxyl terminus were swapped showed membrane affinites similar to those of s26 and s-modulin, respectively. the membrane affinity of an s-modulin mutant lacking c-terminal positive ch ... | 1999 | 9930992 |
| pregnancy-associated glycoprotein and decreased polymorphonuclear leukocyte function in early post-partum dairy cows. | phagocytosis and oxidative burst activity of polymorphonuclear neutrophil leukocytes (pmn) isolated from blood and pregnancy-associated glycoprotein (bpag) concentrations in plasma were evaluated in two longitudinal studies in dairy cows from 3 weeks before until 5 weeks after calving, carried out in the united states and in europe. ingestion of staphylococcus aureus by blood pmn increased during the first week after calving and normalised 3 weeks post-partum. phagocytosis of escherichia coli di ... | 1999 | 9950353 |