Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| cellular requirements for anti-dna production induced in mice by immunization with bacterial dna. | to further define dna immunization as a model for anti-dna production, we investigated the cellular requirements for this response in mice immunized with single-stranded dna from e. coli. the anti-dna responses of genetically immune-deficient mice and congenic controls were measured by elisa after immunization with e. coli dna as complexes with methylated bovine serum albumin in complete freund's adjuvant. t cell-deficient balb/c-nu/nu mice failed to produce igg anti-dna by this protocol despite ... | 1990 | 2209690 |
| preinfection functions of blood polymorphonuclear leukocytes and the outcome of experimental escherichia coli mastitis in the cow. | the relationship between preinfection functions of blood neutrophils and outcome of experimental escherichia coli mastitis was studied in 11 cows. random migration, chemotaxis, phagocytosis, and chemiluminescence by neutrophils were determined in white blood cell suspensions, and in purified neutrophil suspensions. the course of e. coli mastitis (10(4) e. coli 0:157 in rear quarters) was monitored using clinical parameters, counts of e. coli in mastitic secretion, and milk production. regression ... | 1990 | 2184174 |
| growth of gram-negative bacteria in dry cow secretion. | gram-negative bacteria (n = 192) isolated from infected bovine mammary glands were tested for growth in a pooled source of dry cow secretion. growth of klebsiella pneumoniae in dry cow secretion was greater than growth of escherichia coli and klebsiella oxytoca. escherichia coli originating during the early dry period exhibited greater growth in dry cow secretion than those originating around calving or during lactation. klebsiella pneumoniae growth did not differ with time of origin of intramam ... | 1990 | 2184175 |
| glycoprotein glycans that inhibit adhesion of escherichia coli mediated by k99 fimbriae: treatment of experimental colibacillosis. | calf diarrhea due to infection by enterotoxigenic escherichia coli was treated by administration of glycoprotein glycans derived from bovine plasma. the glycan moieties of the nonimmunoglobulin fraction of plasma mimicked the oligosaccharide moiety of intestinal receptors recognized by k99 pili. these glycoprotein glycans inhibited adhesion of e. coli k99+ st+ to erythrocyte glycoconjugates in vitro, and they protected colostrum-deprived newborn calves against lethal doses of enterotoxigenic e. ... | 1990 | 2403535 |
| increased sensitivity of the rapid hydrophobic grid membrane filter enzyme-labeled antibody procedure for escherichia coli o157 detection in foods and bovine feces. | several strains of escherichia coli o157:h7 artificially inoculated into vegetables and dairy products were recovered on hydrophobic grid membrane filters and enumerated by an enzyme-labeled antibody assay. the mean of the recoveries from 12 fresh vegetables was 108.8%, whereas that from 10 dairy products was 93.2%. modified tryptic soy broth at 43 degrees c with shaking at 100 rpm provided optimum recovery of the organism from meat, with a sensitivity of less than or equal to 1 cfu/g, which is ... | 1990 | 2268161 |
| a statewide outbreak of escherichia coli o157:h7 infections in washington state. | in november 1986, a statewide outbreak of escherichia coli o157:h7 infections in washington state was identified after a physician in an eastern washington community hospitalized three patients with hemorrhagic colitis which progressed to thrombotic thrombocytopenic purpura. epidemiologic investigation identified 37 cases in this community and linked the illnesses to a local restaurant which had served ground beef that was the suspected initial vehicle of transmission. the plasmid profile and to ... | 1990 | 2196790 |
| translation of preprochymosin in vitro. evidence for folding of prochymosin to the native conformation. | 1. the cdna coding for preprochymosin has been sub-cloned into the transcription/translation vector pgem-3z, the t7 promoter used to transcribe the gene and the product expressed in an 'in vitro' cell-free system comprising rabbit reticulocyte lysate and dog pancreatic microsomes. 2. translations in various conditions, and analyses of the translation product in reducing and non-reducing conditions, indicate that oxidizing translation conditions and the cleavage of the n-terminal 'pre-' sequence ... | 1990 | 2268293 |
| hemagglutination and hemolysis by escherichia coli isolated from bovine intramammary infections. | seventy-six escherichia coli isolated from bovine intramammary infections were tested for hemagglutination and hemolysis of erythrocytes. fifty-seven percent of isolates were hemagglutination-positive for bovine erythrocytes compared with 46% that agglutinated guinea pig erythrocytes. twenty-eight percent of isolates were hemagglutination-positive for erythrocytes from both species. only 14.5 and 2.6% of isolates were mannose-resistant, hemagglutination-positive for bovine and guinea pig erythro ... | 1990 | 2273142 |
| protein stability and electrostatic interactions between solvent exposed charged side chains. | to investigate the contribution to protein stability of electrostatic interactions between charged surface residues, we have studied the effect of substituting three negatively charged solvent exposed residues with their side-chain amide analogs in bovine calbindin d9k--a small (mr 8,500) globular protein of the calmodulin superfamily. the free energy of urea-induced unfolding for the wild-type and seven mutant proteins has been measured. the mutant proteins have increased stability towards unfo ... | 1990 | 2217161 |
| modulation of resistance to mastitis pathogens by pretreatment of mice with t-2 toxin. | t-2 toxin, a secondary metabolite of fusarium species, is a mycotoxin with immunomodulatory activity. in the present investigation the effects of t-2 toxin on host resistance was studied. the virulence of escherichia coli and staphylococcus aureus in the mammary glands of mice treated with t-2 toxin was compared with their virulence in control mice. virulence was estimated from the ability to induce various types of lesions and bacterial growth in the mammary gland. pretreatment of mice with a s ... | 1990 | 2276697 |
| the three-dimensional structure of recombinant bovine chymosin at 2.3 a resolution. | the crystal structure of recombinant bovine chymosin (ec 3.4.23.4; renin), which was cloned and expressed in escherichia coli, has been determined using x-ray data extending to 2.3 a resolution. the crystals of the enzyme used in this study belong to the space group i222 with unit cell dimensions alpha = 72.7 a, b = 80.3 a, and c = 114.8 a. the structure was solved by the molecular replacement method and was refined by a restrained least-squares procedure. the crystallographic r factor is 0.165 ... | 1990 | 2217166 |
| genetic reconstruction and characterization of the recombinant transacylase (e2b) component of bovine branched-chain alpha-keto acid dehydrogenase complex. implication of histidine 391 as an active site residue. | genetically altered transacylase (e2b) proteins of the bovine branched-chain alpha-keto acid dehydrogenase complex were overexpressed in escherichia coli and characterized. deletion by psti or bal31 digestion of the amino-terminal region of the inner-core domain (residues 175-421) beyond residue 209 resulted in a complete loss of transacylase activity. the enzyme assay was carried out using [1-14c]isovaleryl-coa and exogenous dihydrolipoamide as substrates. the removal of 4 residues (thr-ile-pro ... | 1990 | 2198287 |
| antibodies to iron-regulated outer membrane proteins of coliform bacteria isolated from bovine intramammary infections. | expression of iron-regulated outer membrane proteins (omp) by escherichia coli and klebsiella pneumoniae initially isolated from bovine intramammary infections (imi) was investigated. additionally, the presence of antibodies in bovine serum and mammary secretion directed against the iron-regulated omp was examined. outer membrane proteins were separated by sodium-dodecyl polyacrylamide electrophoresis. detection of immunoglobulin g directed against omp was by immunoblotting. all gram-negative ba ... | 1991 | 1866891 |
| the effect of lipopolysaccharide on bovine mammary macrophage function. | the effect of escherichia coli lipopolysaccharide (lps) on the expression of major histocompatibility complex (mhc) class ii molecules by bovine mammary macrophages was examined. the ability of lps-treated mammary macrophages to support antigen-specific t-cell proliferation, as a measure of their antigen presentation ability, was also evaluated. for this purpose, control and lps-treated macrophages were pulsed with heat-killed staphylococcus aureus and then cultured with s. aureus-sensitized t-c ... | 1991 | 1889031 |
| conglutinin exhibits a complement-dependent enhancement of the respiratory burst of phagocytes stimulated by e. coli. | conglutinin is a mammalian c-type lectin which shows anti-bacterial activity when tested in vivo and in vitro. this study concerns the effect of conglutinin on the respiratory burst of murine spleen cells, using a chemiluminescence assay for measurement of generated reactive oxygen metabolites. conglutinin enhances, in a dose-dependent manner, the respiratory burst of spleen cells stimulated with serum-opsonized escherichia coli. the enhancement was only demonstrable in the presence of a functio ... | 1991 | 1783427 |
| prevalence of antibiotic-resistant escherichia coli in danish pigs and cattle. | the present paper reports on the antibiotic resistance of e. coli isolated from danish piglets and calves in 1987-1988, and compares the results with similar investigations performed during the periods 1971-1972 and 1977-1978. rectal swabs from 52 piglets and from 78 calves were examined. all the animals studied harboured resistant e. coli. this is a significant increase compared to the previously conducted investigations. the number of strains having three or more resistance markers did not dif ... | 1991 | 1772647 |
| in vitro studies on mild alkali induced lesions in dna. | s1 nuclease hydrolysis, benzoylated naphthoylated deae cellulose (bnd-cellulose) chromatography as well as certain immunological and genetic techniques have been used to evaluate the effect of mild alkali (ph10.0) on the dna molecule. native calf thymus dna after exposure to alkaline ph10.0 when subjected to s1 nuclease hydrolysis released significant amount of acid soluble nucleotides as compared to the untreated control. with pbr322 dna, the population of linear dna species increased on s1 dig ... | 1991 | 1789792 |
| expression of bovine inhibin beta subunit in escherichia coli. | 1. a dna fragment encoding the beta subunit of bovine inhibin was amplified using the polymerase chain reaction and was cloned in plasmids puc8 and pur291. 2. cultures of escherichia coli tg2 harbouring pkdk37, a pur291-derived recombinant plasmid, produced a novel protein with a molecular weight of 130,000 corresponding to a beta-galactosidase-inhibin beta fusion protein. 3. the fusion protein was purified from inclusion bodies by solubilization in 8 m urea followed by an ion-exchange and gel p ... | 1991 | 1794452 |
| nitric oxide from vascular smooth muscle cells: regulation of platelet reactivity and smooth muscle cell guanylate cyclase. | 1. incubation of smooth muscle cells (smc) from bovine aorta for 3 min with human washed platelets treated with indomethacin (10 microm) promoted a cell number-related inhibition of platelet aggregation induced by thrombin (40 mu ml-1). this inhibition was not attributable to products of the cyclo-oxygenase pathway for the smc were also treated with indomethacin (10 microm). 2. the inhibitory activity of the smc on platelet aggregation was enhanced by incubating the smc with e. coli lipopolysacc ... | 1991 | 1724627 |
| protective effect of tissue ferritins in experimental escherichia coli infection of mice in vivo. | the effect of ferritins from horse (fh) and bovine (fb) spleen and murine liver (fm) on the survival rate of cfw mice lethally infected with escherichia coli (strain 8440-78 k 80/b) was evaluated. ferritins given intravenously 24 h before intravenous inoculation of bacteria, protected mice most effectively from death due to infection. the effect was dose dependent. at 500 micrograms of ferritin per mouse, the maximum survival rates were 86% (fh), 81% (fm) and 79% (fb), while only 5% of the contr ... | 1991 | 1768608 |
| virulence factors associated with cytotoxic necrotizing factor type two in bovine diarrheic and septicemic strains of escherichia coli. | forty-three bovine isolates of escherichia coli producing a second type of cytotoxic necrotizing factor (cnf2) and three k-12 strains carrying different vir plasmids coding for cnf2 were tested for the presence of several virulence factors. most of the strains were serum resistant (79%), produced an aerobactin (70%), and adhered to calf villi (53%); some of them produced a colicin (32%) and a hemolysin (9%). these strains were also tested by a colony hybridization assay with gene probes for six ... | 1991 | 1774259 |
| comparative immunochemical analysis of the antigenic structures of the dna-binding histone-like hu protein from escherichia coli and eukaryotic histones. | the general antigenic structures of the dna-binding hu protein from e. coli, histones h1, h2a, h2b, h3, and h4 from calf thymus, and histone h5 from chick erythrocytes were compared in immunoassays with the aid of monospecific polyclonal antibodies to the hu protein and the individual histones. a partial cross-reaction between the hu protein and antibodies to histones h1 and h5 was demonstrated. the reaction titres obtained in a solid-phase enzyme immunoassay indicated that the cross-reactions w ... | 1991 | 1797140 |
| an f41-k88-related genetic determinant of bovine septicemic escherichia coli mediates expression of cs31a fimbriae and adherence to epithelial cells. | a genetic determinant related to that encoding the f41 fimbrial adhesin was cloned from a bovine septicemic isolate of escherichia coli. this determinant was found to mediate expression of morphologically distinct fimbriae in e. coli hb101. the gene encoding the fimbrial subunit protein was identified, and the nucleotide sequence was determined. homology with the amino-terminal amino acid sequence of cs31a (j. girardeau, m. der vartanian, j. ollier, and m. contrepois, infect. immun. 56:2180-2188 ... | 1991 | 1675627 |
| hp 0.35, a cephalosporin degradation product is a specific inhibitor of lentiviral rnases h. | penicillins, cephalosporins and other betalactam antibiotics are widely used antibacterial drugs. recently it was found that some of them also have effects on proliferating eukaryotic cells (neftel, k.a. and hübscher, u. (1987) antimicrob. agents chemother. 31, 1657-1661), and one such effect was shown to be the inhibition of dna polymerase alpha (huynh do,u., neftel, k.a., spadari, s. and hübscher, u. (1987) nucl. acids res. 15, 10495-10506). the data suggested that degradation products of beta ... | 1991 | 1714562 |
| modulation of function of bovine polymorphonuclear leukocytes and lymphocytes by high temperature in vitro and in vivo. | function of polymorphonuclear leukocytes (pmnl) and proliferation of lymphocytes after stimulation with mitogens were evaluated in vitro at incubation temperatures of 38.5 and 42 c, and after in vivo heat stress of lactating holstein cows. cytochrome-c reduction and random migration of pmnl were reduced when cells were preincubated or incubated at 42 c, but high incubation temperature had little or no effect on phagocytosis and killing of escherichia coli. proliferation of lymphocytes was reduce ... | 1991 | 1662922 |
| isolation of a cdna encoding a mammalian multiubiquitinating enzyme (e225k) and overexpression of the functional enzyme in escherichia coli. | the ubiquitin (ub)-conjugating enzyme e2(25k) catalyzes the synthesis of multi-ub chains in which successive ub units are linked by an isopeptide bond involving the epsilon-amino group of lys-48 of ubn, and the cooh-terminal gly residue of ubn+1 (chen, z., and pickart, c. m. (1990) j. biol. chem., 265, 21835-21842). we now describe the polymerase chain reaction (pcr)-based cloning of an e2(25k)-encoding cdna from a bovine thymus library, using degenerate oligonucleotide primers based on the sequ ... | 1991 | 1714895 |
| molecular cloning and expression of biologically active human glia maturation factor-beta. | glia maturation factor-beta, a protein found in the brains of all vertebrates thus far examined, appears to play a role in the differentiation, maintenance, and regeneration of the nervous system. using oligonucleotide probes based on the sequences of three tryptic peptides derived from bovine glia maturation factor-beta, we screened a human brainstem cdna library in lambda gt11. a 0.7-kb clone was isolated, sequenced in its entirety, and found to encode a polypeptide of 142 amino acids which co ... | 1991 | 1712830 |
| thrombin and h64a subtilisin cleavage of fusion proteins for preparation of human recombinant parathyroid hormone. | human parathyroid hormone, hpth, an 84 amino acid polypeptide, was produced intracellularly in escherichia coli as a fusion protein, linked to the c-terminus of a 15 kd igg-binding protein. approximately 100 mg fusion protein was obtained per liter fermentation medium. to test the efficiency of two alternative enzymatic cleavage methods, two fusion proteins differing only in the linker region were constructed. cleavage of a phe-phe-pro-arg linker was obtained with bovine thrombin and cleavage of ... | 1991 | 1799410 |
| potent antibacterial peptides generated by pepsin digestion of bovine lactoferrin. | the antibacterial properties of enzymatic hydrolysates of bovine lactoferrin were examined to determine whether active peptides are produced from this protein. hydrolysates prepared by cleavage of lactoferrin with porcine pepsin, cod pepsin, or acid protease from penicillium duponti showed strong activity against escherichia coli o111, whereas hydrolysates produced by trypsin, papain, or other neutral proteases were much less active. low molecular weight peptides generated by porcine pepsin clea ... | 1991 | 1787185 |
| controlling acute escherichia coli mastitis during the periparturient period with recombinant bovine interferon gamma. | the efficacy of recombinant bovine interferon (rboifn)-gamma against experimentally induced escherichia coli mastitis during the periparturient period was investigated. dairy cows intramammarily treated with rboifn-gamma 24 h before the e. coli challenge had fewer infected quarters, lower clinical scores, and infections of shorter duration when compared to placebo-treated animals. all rboifn-gamma treated cows survived the experimental e. coli challenge. however, placebo treated cows had a 42% m ... | 1991 | 1908159 |
| impact of transfer from animal-source insulins to biosynthetic human insulin (rdna e coli) in patients with diabetes mellitus. | six hundred forty-eight patients (50.5% men; 49.5% women) with diabetes mellitus on animal-source insulin therapy for at least five years were studied. in this patient population, approximately 68.7% had type i insulin-dependent diabetes mellitus and 31.3% had type ii noninsulin-dependent diabetes mellitus, nonetheless requiring insulin therapy. patients were voluntarily transferred from animal-source insulin to biosynthetic human insulin derived by recombinant dna technology from genetically al ... | 1991 | 1799920 |
| simian virus 40 vp2/3 small structural proteins harbor their own nuclear transport signal. | we have used a microinjection approach to identify a domain of the simian virus 40 (sv40) structural proteins vp2 and vp3(vp2/3) responsible for their nuclear transport. by using both synthetic peptides, containing small regions of vp2/3 conjugated to bovine serum albumin (bsa), and beta-galactosidase-vp3 fusion proteins, we have narrowed this nuclear transport signal (nts) to 9 amino acids (198 to 206 of vp3 or 316 to 324 of vp2), gly-pro-asn-lys-lys-lys-arg-lys-leu. the porter proteins carryin ... | 1991 | 1847270 |
| the amino-terminal half of rotavirus sa114fm vp4 protein contains a hemagglutination domain and primes for neutralizing antibodies to the virus. | we have previously reported the synthesis in escherichia coli of polypeptide ms2-vp8', which contains the amino-terminal half of the sa114fm vp4 protein fused to ms2 bacteriophage polymerase sequences (c. f. arias, m. lizano, and s. lópez, j. gen. virol. 68:633-642, 1987). in this work we have synthesized the carboxy-terminal half of the vp4 protein also fused to the ms2 polymerase. this protein, designated ms2-vp5', was recognized by sera to the complete virion and was able to induce antibodies ... | 1991 | 1847459 |
| glycosylation of insulin-like growth factor binding protein-3 (igfbp-3) is not required for potentiation of igf-i action: evidence for processing of cell-bound igfbp-3. | insulin-like growth factor binding protein-3 (igfbp-3) is unique among the igf binding proteins in its extensive glycosylation in the native state. to determine the functional significance of carbohydrate moieties on igfbp-3, we examined the effects of nonglycosylated escherichia coli-derived recombinant human igfbp-3 (higfbp-3e. coli) and glycosylated chinese hamster ovary cell-derived higfbp-3 (higfbp-3cho) on igf-i action in cultured bovine fibroblasts. both higfbp-3 preparations bound igf-i ... | 1991 | 1720092 |
| n-acetyl-beta-d-glucosaminidase, etiologic agent, and duration of clinical signs for sequential episodes of chronic clinical mastitis in dairy cows. | this study examined effects of repeated episodes of clinical mastitis in chronically infected quarters on milk n-acetyl-beta-d-glucosaminidase activity and duration of clinical signs. milk samples were collected at each clinical onset from 49 chronic mastitis cases on a 1700-cow michigan dairy farm. there were 49 first episodes of clinical mastitis, 49 second episodes, and 13 episodes of third or more. agents isolated were staphylococcus aureus (18.4%), staphylococcus (7.3%), no growth (20.2%), ... | 1991 | 1880262 |
| escherichia coli o157:h7 and its significance in foods. | escherichia coli o157:h7 was conclusively identified as a pathogen in 1982 following its association with two food-related outbreaks of an unusual gastrointestinal illness. the organism is now recognized as an important cause of foodborne disease, with outbreaks reported in the u.s.a., canada, and the united kingdom. illness is generally quite severe, and can include three different syndromes, i.e., hemorrhagic colitis, hemolytic uremic syndrome, and thrombotic thrombocytopenic purpura. most out ... | 1991 | 1854598 |
| interaction of animal mitochondrial ef-tu.ef-ts with aminoacyl-trna, guanine nucleotides, and ribosomes. | the mammalian mitochondrial complex consisting of elongation factors ef-tu and ef-ts (ef-tu.tsmt) is capable of efficiently binding aminoacyl-trna to the ribosome in the presence and absence of guanine nucleotides. in the presence of gtp the binding reaction is catalytic. in the absence of guanine nucleotides, or in the presence of a non-hydrolyzable gtp analog, only one round of ribosome binding occurs. ef-tu.tsmt is capable of forming a ternary complex with gtp and escherichia coli phe-trna as ... | 1991 | 1885567 |
| effect of milking without pulsation on teat duct colonization with streptococcus agalactiae and penetrability to endotoxin. | forty quarters of 10 cows were milked for a 9-d period with one of four treatments: 1) no liner pulsation, 2) conventional milking, 3) no pulsation for 4 d followed by conventional milking for 5 d, and 4) conventional milking for 4 d followed by milking without pulsation for 5 d. all teat orifices were inoculated with approximately .5 million cfu of streptococcus agalactiae and streptococcus dysgalactiae on d 1 and 5. recoveries of strep. agalactiae from the teat end were increased for teats mil ... | 1991 | 1779054 |
| [coliform bacilli which produce cytotoxins: importance in veterinary medicine and public health]. | this article reviews published data (october 1st, 1990) on 4 types of cytotoxins produced by animal and human pathogenic strains of e coli, ie, verotoxins, also named shiga-like toxins, cytotoxic necrotizing factors, haemolysin, and cytolethal distending toxin. the biological and molecular properties are described for each type of cytotoxin, the association of producing strains with animal pathology, and the role of domestic animals as reservoirs of strains pathogenic for man. | 1991 | 1897865 |
| analyzing the substrate specificity of saccharomyces cerevisiae myristoyl-coa:protein n-myristoyltransferase by co-expressing it with mammalian g protein alpha subunits in escherichia coli. | a dual plasmid system was used to examine the protein and acyl-coa specificities of saccharomyces cerevisiae myristoyl-coa:protein n-myristoyltransferase (nmt) by co-expressing it in escherichia coli with each of four homologous alpha subunits of the signal-transducing, heterotrimeric g proteins. exogenous [3h]myristate was incorporated into rat gi alpha 1 and rat go alpha but not into bovine gs alpha or human gz alpha. oxygen for methylene group substitutions in myristate result in analogs with ... | 1991 | 1903791 |
| dna hybridization and latex agglutination for detection of heat-labile- and shiga-like toxin-producing escherichia coli in meat. | dna-hybridization and latex-agglutination tests were used for screening of a group of escherichia coli isolates for heat-labile enterotoxin (lt)- and shiga-like toxin (slt1 or vt1) -producing strains, respectively. strains tested originated from 162 meat samples (poultry, pigs and beef) chosen at random. additionally lt- and slt1-producing reference strains were tested. the dna-hybridization technique allowed screening of large numbers of strains, whereas large scale testing of strains by latex ... | 1991 | 1863526 |
| antigenic homology among gram-negative organisms isolated from cattle with clinical mastitis. | this study examined the degree of serologic homology among mastitis pathogens. antibodies were raised against the rc mutant, escherichia coli o111:b4 (strain j5) and affinity purified against lipopolysaccharide derived from the ra mutant, salmonella typhimurium tv119. these antibodies reacted with a battery of unrelated gram-negative bacteria in whole cell elisa. bacteria with strong cross-reactions included a heterologous, smooth e. coli, salmonella dublin, s. typhimurium, salmonella newport, a ... | 1991 | 1907303 |
| rsr1 and rap1 gtpases are activated by the same gtpase-activating protein and require threonine 65 for their activation. | the rsr1 protein of saccharomyces cerevisiae has been shown to be essential for bud site selection (bender, a., and pringle, j. (1989) proc. natl. acad. sci. u.s.a. 86, 9976-9980). this protein of 272 amino acids shares approximately 50% sequence identity with both ras and rap gtpases. however, neither gtp binding nor gtpase activity of the rsr1 protein has been reported. the rsr1 protein shares with human rap1 gtpases the four specific motifs, i.e. gly-12, residues 32-40, ala-59, and residues 6 ... | 1991 | 1910037 |
| [the immunogenicity of mono- and associated vaccines made from strains of opportunistic bacteria]. | in experiments on white mice the immunogenic potency of two variants of combined vaccines prepared from strains of opportunistic bacteria was compared with that of monovaccines used as the components of the combined vaccines. all the vaccines under study were found highly immunogenic. decrease of monovaccine share in the combined vaccine did not reduce the immunogenic potency to any of the strains. an 8- to 16-fold increase in the level of specific antibodies in the colostric lactosera of cows i ... | 1991 | 1801485 |
| pathogenic escherichia coli o157:h7 and their detection. | enterohaemorrhagic escherichia coli o157:h7 is of major concern to the food industry due to high pathogenicity of this foodborne organism. for the detection of these bacteria a special agar medium with a fluorogenic substrate has been developed. the medium uses the characteristics of this e. coli serotype not to ferment sorbitol and not to produce beta-glucuronidase. in contrast, approximately 96% of all other strains of e. coli are sorbitol-positive and nearly all of them are beta-glucuronidase ... | 1991 | 1817428 |
| development of genomic probes to sarcocystis cruzi (apicomplexa). | a genomic library of sarcocystis cruzi sporozoite dna was constructed in bacteriophage lambda gt10. recombinant phages containing insert dna were selected by growth on escherichia coli strain c600 hfla150. of 14 clones examined, 11 contained dna inserts ranging in size from approximately 1.45 kilobase (kb) to 6.18 kb. insert dna from four of these clones specifically hybridized to 32p-labelled s. cruzi merozoite dna. one of these insert dna, clone sl41, was selected and labelled with 32p. this p ... | 1991 | 1837193 |
| interactions of the bovine brain a1-adenosine receptor with recombinant g protein alpha-subunits. selectivity for rgi alpha-3. | the ability of the bovine brain a1-adenosine receptor to discriminate between different g protein subtypes was tested using g protein alpha-subunits synthesized in escherichia coli (rg alpha-subunits). when combined with a 3-fold molar excess of beta gamma-subunit purified from bovine brain and used at high concentrations, all three subtypes of rgi alpha (rgi alpha-1, rgi alpha-2, and rgi alpha-3) and rgo alpha were capable of reconstituting guanine nucleotide-sensitive high-affinity binding of ... | 1991 | 1917922 |
| immunoreactive alpha a crystallin in rat non-lenticular tissues detected with a sensitive immunoassay method. | for the quantitative analysis of the a subunit of alpha crystallin (alpha a) in the lens and for the survey of possible existence of alpha a in the non-lenticular tissues, we have established a highly sensitive and specific immunoassay method for alpha a. antisera to alpha a were raised in rabbits with alpha a purified from bovine lens, or the c-terminal decapeptide (eekpssapss) of alpha a (alpha apep). the antibodies to alpha a and alpha apep were purified by the use of an alpha a-coupled sepha ... | 1991 | 1932094 |
| a new diarrhoeic syndrome with ataxia in young charolais calves: clinical and microbiological studies. | a new diarrhoeic syndrome was examined clinically in 19 one to two-week old charolais calves. it differs from other digestive disorders in calves of this age in the discrete diarrhoeic signs, the absence of dehydration and the presence of signs of ataxia. the microbiological study carried out for three consecutive years in 58 sick calves and nine healthy control calves demonstrated the special role of e coli possessing virulence markers from septicaemic strains (cs31a, col v). the clinical signs ... | 1991 | 1853535 |
| endometrial biopsy in holstein-friesian dairy cows. iii. bacteriological analysis and correlations with histological findings. | this study examines the results of bacterial culture from 159 endometrial biopsy samples from 97 commercial dairy cows and correlations between bacteriological and histological findings. bacteria were isolated from approximately 80% of biopsies taken at day 26 and day 40 postpartum. eleven percent of biopsies were positive for both aerobic and anaerobic culture. streptococci, escherichia coli and actinomyces pyogenes were the most common isolates. isolation of a. pyogenes from a biopsy at day 26 ... | 1991 | 1884297 |
| sensitive immunoassay for rat parvalbumin: tissue distribution and developmental changes. | a sensitive enzyme immunoassay for measurements of rat parvalbumin was established using antibodies raised in rabbits with parvalbumin purified from skeletal muscles. antibodies in the antiserum were purified with a parvalbumin-coupled sepharose column. the sandwich-type immunoassay system for parvalbumin was composed of polystyrene balls with immobilized purified antibodies and the same antibodies labeled with beta-d-galactosidase from escherichia coli. the assay was highly sensitive and the mi ... | 1991 | 1892868 |
| study of mammalian selenocysteyl-trna synthesis with [75se]hse. | the mechanisms of the synthesis of mammalian selenocysteyl-(scy)-trna were studied using [75se]h2se. h2se was prepared from [75se]selenite, glutathione, nadph and glutathione reductase, and was purified by chromatography. it was confirmed that this h2se was a se donor in the reaction of the synthesis of scy-trna. [75se]scy, liberated from aminoacyl-trna, was analyzed by tlc on silica gel an subsequent autoradiography. the activity of scy-trna synthesis was found in the supernatant at 105,000 x g ... | 1991 | 1894009 |
| effect of site-directed mutagenic alterations on adp-ribosyltransferase activity of the a subunit of escherichia coli heat-labile enterotoxin. | previous studies of the s1 subunit of pertussis toxin, an nad(+)-dependent adp-ribosyltransferase, suggested that a small amino-terminal region of amino acid sequence similarity to the active fragments of both cholera toxin and escherichia coli heat-labile enterotoxin represents a region containing critical active-site residues that might be involved in the binding of the substrate nad+. other studies of two other bacterial toxins possessing adp-ribosyltransferase activity, diphtheria toxin and ... | 1991 | 1908825 |
| cryptosporidiosis in neonatal calves: 277 cases (1986-1987). | from january 1986 through december 1987, 277 cases of cryptosporidiosis in calves were diagnosed by the south dakota state university diagnostic laboratory. cryptosporidium sp was the only pathogen identified in 142 (51.3%) of the calves. concurrent infections with rotavirus, coronavirus, escherichia coli, salmonella sp, bovine viral diarrhea virus, or other pathogens were identified in the remaining 135 calves. after elimination of cases involving autolyzed specimens or calves with chronic diar ... | 1991 | 1874678 |
| partial budget analysis of vaccinating dairy cattle against coliform mastitis with an escherichia coli j5 vaccine. | partial budget analysis of clinical coliform mastitis prevention supported vaccination of dairy cows with an escherichia coli j5 vaccine. increased profits of $57/cow lactation were predicted using a computer spreadsheet derived partial budget with generalized herd input data. herd vaccination programs were predicted to be profitable when greater than 1% of cow lactations resulted in clinical coliform mastitis. herd vaccination programs were predicted to be profitable at all herd milk production ... | 1991 | 1917656 |
| identification of enteropathogenic escherichia coli isolated in britain as enteroaggregative or as members of a subclass of attaching-and-effacing e. coli not hybridising with the epec adherence-factor probe. | strains of escherichia coli from sporadic cases of diarrhoea and belonging to serotypes o44:h18, o55:h7, o111ab:h21, o111ab:h25 or o126:h27 were examined for virulence properties. with the exception of o111ab:h25 these are considered to be classical enteropathogenic e. coli (epec) serotypes. the strains had been isolated in britain from the faeces of children less than 3 years old. of the serotypes examined, 7 of 13 o44:h18 strains, all of 10 o111ab:h21 strains and 13 of 21 o126:h27 strains belo ... | 1991 | 1941998 |
| cloning and sequence analysis of the human liver rhodanese: comparison with the bovine and chicken enzymes. | the cdna for the human rhodanese (thiosulfate: cyanide sulfurtransferase, ec 2.8.1.1), a nuclearly encoded protein of the mitochondrial matrix, was isolated from a human fetal liver cdna library. nucleotide sequence revealed an open reading frame coding for a polypeptide of 295 amino acids, which presented a 57% and 58% identity with the bovine and avian rhodanese, respectively. the analysis of the 5'-ends of the coding region gave no evidence for the presence of a cleavable signal sequence as f ... | 1991 | 1953758 |
| [synthesis of the e-antigen of the hepatitis b virus (hbeag) in eukaryotic cells by a recombinant strain of the vaccinia virus]. | the recombinant plasmids containing the gene for hepatitis b viral core-antigen with the pre-core-sequence controlled by the early-late promoter of the 7.5' k protein gene were constructed. the recombinant strains of vaccinia virus were obtained on their basis (vhbe42-1 and vhbe42-3) selectively expressing hbeag of hepatitis b virus. the kinetics of hbeag synthesis was studied in infected cells as well as secretion of the protein into culturing medium. three proteins were found by blotting techn ... | 1991 | 1745274 |
| use of probes to detect virulence factor dna sequences in escherichia coli strains isolated from foods. | escherichia coli strains were isolated from 96 food samples (32 milks, 4 dairy products, 36 raw meats, 7 meat products, 7 sandwiches and 10 ready-to-eat meals). a total of 306 colonies was submitted to hybridization assays with dna probes for the following virulence factors: heat-labile toxins (lt-i and lt-ii), heat-stable toxins (st-h and st-p). shiga-like toxins (slt-i and slt-ii), adherence factor of enteropathogenic e. coli (eaf) and invasive factor (inv). six colonies isolated from 4 food s ... | 1991 | 1677256 |
| stimulation of dna polymerase alpha activity by microtubule-associated proteins. | microtubule-associated protein 2 (map2) isolated from porcine brains stimulated the activity of dna polymerase alpha immunopurified from calf thymus or human lymphoma cells, in a dose-dependent manner. this stimulation was pronounced when activated dna or poly(da).(dt)10 was used as the template-primer. dna polymerase alpha bound to a map2-immobilized column, whereas preincubation of the enzyme with unbound map2 prevented binding to the column. these events suggested that a physical binding occu ... | 1991 | 1742279 |
| swine and cattle enterotoxigenic escherichia coli-mediated diarrhea. development of therapies based on inhibition of bacteria-host interactions. | enterotoxigenic escherichia coli (etec) frequently occurs in diarrheal disease afflicting domestic animals. in this paper is summarized the research carried out over the last decade on the two important determinants of virulence that plays a role in the development of the infection, namely the colonizing ability of the small intestine mediated by specific fimbrial adhesins acting as lectins and the production of enterotoxins. recent progress in knowledge of the phenomenon led to alternative stra ... | 1991 | 1723693 |
| cloning, expression and characterization of the human transcription elongation factor, tfiis. | the cdna for the human elongation factor, tfiis, has been cloned and expressed in e. coli with the t7 expression system. this 280-amino acid tfiis protein is shorter by 21 residues than that of the mouse. the missing 21 residues are located in the amino-terminal region, which is not thought to be required for transcriptional stimulation. apart from this gap, human and mouse proteins reveal 96% overall identity and 98.5% sequence similarity if conservative substitutions are taken into account. th ... | 1991 | 1708494 |
| phospholipase a2 engineering. x-ray structural and functional evidence for the interaction of lysine-56 with substrates. | site-directed mutagenesis studies of bovine pancreatic phospholipase a2 (pla2, overproduced in escherichia coli) showed that replacement of surface residue lys-56 by a neutral or hydrophobic amino acid residue resulted in an unexpected and significant change in the function of the enzyme. the kcat for phosphatidylcholine micelles increases 3-4-fold for k56m, k56i, and k56f and ca. 2-fold for k56n and k56t but does not change for k56r. these results suggest that the side chain of residue 56 has s ... | 1991 | 1751497 |
| potent bactericidal activity of bovine lactoferrin hydrolysate produced by heat treatment at acidic ph. | a hydrolysate of bovine lactoferrin produced by heat treatment under acidic conditions had antibacterial activity at concentrations of 10 micrograms/ml in the culture medium. the optimal degree of hydrolysis for this activity was about 10%. heat-treated lactoferrin, treated at ph 2.0 and 120 degrees c for 15 min and degree of hydrolysis of about 10%, had no fe-binding capacity (0%) and less antigenicity (about 10(-6) than untreated lactoferrin. heat-treated lactoferrin increased in antibacterial ... | 1991 | 1757616 |
| rapid procedure for detecting enterohemorrhagic escherichia coli o157:h7 in food. | a sensitive, specific procedure was developed for detecting escherichia coli o157:h7 in food in less than 20 h. the procedure involves enrichment of 25 g of food in 225 ml of a selective enrichment medium for 16 to 18 h at 37 degrees c with agitation (150 rpm). the enrichment culture is applied to a sandwich enzyme-linked immunosorbent assay (elisa) with a polyclonal antibody specific for e. coli o157 antigen as the capture antibody and a monoclonal antibody specific for enterohemorrhagic e. col ... | 1991 | 1768144 |
| characterization of a new fimbrial antigen present in escherichia coli strains isolated from calves. | thirteen escherichia coli strains isolated from calves with diarrhoea, supposed to carry a common antigen were examined for their hemagglutinating activity and compared by bacterial agglutination, double diffusion in two dimensions and by crossed immunoelectrophoresis (cie). two of the strains were examined also in the electron microscope. most of the strains agglutinated red blood cells of horse, ox, guinea pig and chicken, of which the agglutination of ox erythrocytes was mannose-resistant (mr ... | 1991 | 1686345 |
| [antibody response in mice, rabbits and pigs in response to vaccination with an inactivated oil vaccine containing rotavirus and escherichia coli strains with k88, k99 and 987p fimbrial antigens]. | in trials with mice, rabbits and weanling piglets, four experimental charges of a combined inactivated oil vaccine against diarrhoeas in mammals were tested: the vaccine was to be implanted to sows and it contained porcine rotavirus (prv); two charges also contained bovine rotavirus and bacterins of enterotoxicogenic strains of e. coli with protective antigens k88, k99 and 987p. at low starting antibody titres the twofold i.m. implantation of 0.2 ml vaccine stimulated in mice the production of a ... | 1991 | 1686823 |
| expression of bovine leukaemia virus antigens fused to ms2 polymerase in e. coli. | the main core protein and segments of envelope proteins of bovine leukaemia virus fused to ms2 polymerase were expressed in e. coli. the synthesis rate varied between 3 and 25% of the total cellular proteins. blv-ms2 polymerase fusion proteins were detected immunologically using rabbit anti-blv sera, monoclonal antibodies and a serum of a blv-infected cow. | 1991 | 1686964 |
| isolation and characterization of the adenylate cyclase structural gene of neurospora crassa. | a single gene (nac) encoding an adenylate cyclase was cloned from the genomic dna library of neurospora crassa, using the dna fragment encoding the catalytic domain of adenylate cyclase of saccharomyces cerevisiae as a probe. the open reading frame of this gene (6900 base pairs) was interrupted three time by introns. the protein encoded consists of 2300 amino acids and has adenylate cyclase activity. n. crassa adenylate cyclase has a high degree of homology with the catalytic domains of yeast an ... | 1991 | 1680356 |
| a bovine papillomavirus e1-related protein binds specifically to bovine papillomavirus dna. | the e1 open reading frame of bovine papillomavirus (bpv) was expressed as a reca-e1 fusion protein in escherichia coli. the bacterially expressed reca-e1 protein exhibited sequence-specific dna binding activity; strong binding to the region from nucleotides 7819 to 93 on the bpv genome (designated region a) and weak binding to the adjacent region from nucleotides 7457 to 7818 (region b) were observed. the interaction between the bpv-derived reca-e1 protein and region a appeared to be highly spec ... | 1991 | 1654443 |
| cdna cloning and expression of bauhinia purpurea lectin. | bauhinia purpurea lectin (bpa) was purified from seeds of b. purpurea alba. the purified lectin was digested with an endoproteinase, asp-n, or trypsin and then the amino acid sequences of the resultant fragments were analyzed. furthermore, a cdna library for bpa was constructed using rna isolated from germinated bauhinia purpurea seeds. by gene cloning, the nucleotide sequence of bpa cdna and its deduced amino acid sequence were analyzed. the cloned bpa cdna comprised 1,152 nucleotides and the o ... | 1991 | 1657898 |
| diverse proteins homologous to inositol monophosphatase. | bovine inositol monophosphatase (imp) and several homologous proteins were found to share two sequence motifs with bovine inositol polyphosphate 1-phosphatase (ipp). these motifs may correspond to binding sites within imp and ipp for inositol phosphates or for lithium, since both substances are bound by these proteins. this suggests that the proteins homologous to imp, which have diverse biological roles but whose function is not clear, may act by enhancing the synthesis or degradation of phosph ... | 1991 | 1660408 |
| dietary selenium effects on milk eicosanoid concentration in dairy cows during coliform mastitis. | the effect of selenium deficiency on the product profile of arachidonic acid oxidation by enzymatic pathways in holstein cows with experimentally-induced coliform mastitis was investigated. the animals were fed dairy rations containing 0.05 mg se/kg dry matter, with the supplemented group receiving additional se to increase the dietary concentration to approximately 0.35 mg se/kg dry matter. cows were inoculated intracisternally with 30 colony-forming-units of escherichia coli at 14-16 weeks of ... | 1991 | 1665569 |
| production of bovine insulin-like growth factor 2 (bigf2) in escherichia coli. | bovine insulin-like growth factor 2 (bigf2) was produced in inclusion bodies in the cytoplasm of escherichia coli and accumulated at high levels: 20-25% of total coomassie-stained bacterial protein. the level of accumulation of bigf2 was affected by the choice of codons in the 5' end of the coding sequence and by a rpoh mutation in the host cells. purified recombinant bigf2 had the native n terminus and the same mitogenic activity as that of bigf2 purified from bovine serum. | 1991 | 2055493 |
| effect of vaccination with the r mutant escherichia coli (j5) antigen on morbidity and mortality of dairy calves. | a prospective cohort study was undertaken to determine the efficacy of escherichia coli 0111:b4 (j5) vaccination in dairy calves. calves on 2 units were vaccinated when they were 2 to 3 days old and 2 weeks later with the j5 antigen or they were left unvaccinated, and were observed during the first 60 days of age for morbidity and mortality. events recorded were death, the first case of illness, the first sign of a respiratory tract condition, the first sign of diarrhea, and the first treatment. ... | 1991 | 1952340 |
| (14-38, 30-51) double-disulphide intermediate in folding of bovine pancreatic trypsin inhibitor: a two-dimensional 1h nuclear magnetic resonance study. | an analogue of the bpti folding intermediate that contains only the disulphide bonds between cys14 and cys38 and between cys30 and cys51 has been prepared in escherichia coli by protein engineering methods. the other two cys residues of native bpti (at positions 5 and 55) have been replaced by ser. essentially complete proton resonance assignments of the analogue were obtained by employing two-dimensional 1h nuclear magnetic resonance techniques. the intermediate has a more extended conformation ... | 1991 | 1960731 |
| antibacterial activities in vitro and in vivo and pharmacokinetics of cefquinome (hr 111v), a new broad-spectrum cephalosporin. | cefquinome is a new injectable aminothiazolyl cephalosporin derivative. it is stable against chromosomally and plasmid-encoded beta-lactamases and has a broad antibacterial spectrum. staphylococcus aureus, streptococci, pseudomonas aeruginosa, and members of the family enterobacteriaceae (escherichia coli, salmonella spp., klebsiella spp., enterobacter spp., citrobacter spp., and serratia marcescens) are inhibited at low concentrations. cefquinome is also active against many strains of methicill ... | 1991 | 2014969 |
| molecular characterization of bovine brain p75, a high affinity binding protein for the regulatory subunit of camp-dependent protein kinase ii beta. | in mammalian brain, physiological signals carried by camp seem to be targeted to intraneuronal sites by the association of camp-dependent protein kinase ii beta with anchoring proteins that bind the regulatory subunit (rii beta) of the enzyme. previously, an rii beta-binding domain was characterized in a large (mr approximately 150,000) candidate anchor protein, rat brain p150 (bregman, d. b., bhattacharyya, n., and rubin, c. s. (1989) j. biol. chem. 264, 4648-4656). rii beta-binding proteins wi ... | 1991 | 2016323 |
| maple syrup urine disease: domain structure, mutations and exon skipping in the dihydrolipoyl transacylase (e2) component of the branched-chain alpha-keto acid dehydrogenase complex. | maple syrup urine disease (msud) is an autosomal recessive disorder in the oxidative decarboxylation of the branched-chain alpha-keto acids derived from leucine, isoleucine and valine. the enzyme deficient in msud, the branched-chain alpha-keto acid dehydrogenase (bckad) complex, is a mitochondrial multienzyme complex consisting of at least six distinct subunits. msud is genetically heterogeneous as manifested by lesions in different subunits of the bckad complex among unrelated patients. to app ... | 1991 | 1943690 |
| the action of 1-nitroso-8-nitropyrene in escherichia coli: dna adduct formation and mutational consequences in the absence of nucleotide excision-repair. | to study the mechanisms of mutagenesis by the carcinogen 1,8-dinitropyrene we have determined the dna adducts formed and mutations induced by its partially activated metabolite 1-nitroso-8-nitropyrene (1,8-nonp) in an escherichia coli strain deficient in nucleotide excision-repair. using dna sequence analysis we have characterized a collection of 159 laci- mutations recovered following treatment with 1,8-nonp. the mutational spectrum was dominated by -1 frameshifts (110 events) in runs of contig ... | 1991 | 2029753 |
| preliminary characterization of a cloned neutral isoelectric form of the human peptidyl prolyl isomerase cyclophilin. | we report the cloning of a neutral isoelectric form of the human peptidyl prolyl isomerase, cyclophilin, its expression in escherichia coli, and its purification and comparison to bovine thymus cyclophilin. the cloned protein exhibited a pi of approximately 7.8 and formed a simple 1:1 complex with cyclosporin a. this cloned form had a pi similar to that observed for the neutral isoform (pi approximately 7.4) of human splenocyte cyclophilin. the bovine thymus proteins exhibited anomalous behavior ... | 1991 | 1989998 |
| initiation of protein synthesis in animal mitochondria. purification and characterization of translational initiation factor 2. | bovine liver mitochondrial translational initiation factor 2 (if-2mt) has been purified to near homogeneity. the scheme developed results in a 24,000-fold purification of the factor with about 26% recovery of activity. sds-polyacrylamide gel electrophoresis indicates that if-2mt has a subunit molecular mass of 85 kda. if-2mt promotes the binding of formyl(f)met-trna to mitochondrial ribosomes but is inactive with the nonformylated derivative. if-2mt is active on chloroplast 30 s ribosomal subuni ... | 1991 | 1939122 |
| cdna and deduced amino acid sequence of a mouse dna repair enzyme (apex nuclease) with significant homology to escherichia coli exonuclease iii. | we purified a mouse dna repair enzyme having apurinic/apyrimidinic endonuclease, dna 3'-phosphatase, 3'-5'-exonuclease and dna 3' repair diesterase activities, and designated the enzyme as apex nuclease. a cdna clone for the enzyme was isolated from a mouse spleen cdna library using probes of degenerate oligonucleotides deduced from the n-terminal amino acid sequence of the enzyme. the complete nucleotide sequence of the cdna (1.3 kilobases) was determined. northern hybridization using this cdna ... | 1991 | 1939131 |
| clonal relationships among escherichia coli serogroup 06 isolates from human and animal infections. | the clonal relationship of thirty e. coli strains of 0 antigen serotype 06 isolated from human, dog, pig or cow infections were investigated. two main clones with serotypes 06 : h1 or 06 : h31, h- were identified. isolates from humans, dogs, pigs and cows were found in both clones, indicating that animals are a possible source for human extraintestinal escherichia coli strains. two human etec (06 : h16) and two pig isolates (06 : h10) were not related to the 06 : h1 or 06 : h31, h- e. coli clone ... | 1991 | 1884980 |
| isolation and characterization of a saccharomyces cerevisiae mutant disrupted for the succinate dehydrogenase flavoprotein subunit. | a partial genomic clone of the flavoprotein subunit of the mitochondrial enzyme, succinate dehydrogenase (ec 1.3.99.1) from saccharomyces cerevisiae has been isolated. the partial clone was used to construct, by targeted gene disruption, a yeast mutant with a defective flavoprotein subunit gene. submitochondrial membranes from the mutant are defective in activities requiring a functional succinate dehydrogenase but not in other respiratory chain activities. in addition, the mutant contains signi ... | 1991 | 1939170 |
| small-angle x-ray scattering studies of calmodulin mutants with deletions in the linker region of the central helix indicate that the linker region retains a predominantly alpha-helical conformation. | two mutant forms of calmodulin were examined by small-angle x-ray scattering in solution and compared with the wild-type protein. each mutant has deletions in the linker region of the central helix: one lacks residues glu-83 and glu-84 (des2) and the other lacks residues ser-81 through glu-84 (des4). the deletions change both the radii of gyration and the maximum dimensions of the molecules. in the presence of ca2+, the observed radii of gyration are 22.4 a for wild-type bacterially expressed ca ... | 1991 | 1991098 |
| the use of plasmid profiles and nucleic acid probes in epidemiologic investigations of foodborne, diarrheal diseases. | the application of nucleic acid analyses to investigations of infectious disease outbreaks has resulted in useful molecular strain markers that distinguish the epidemic clone of a particular pathogen and help identify specific vehicles of infection. we have successfully used plasmid profile analysis, restriction endonuclease digestion of plasmid and whole-cell dnas, and nucleic acid hybridization to investigate recent outbreaks of foodborne diarrheal illness. plasmid analysis has been important ... | 1991 | 2018708 |
| mutations in the heparin-binding domains of human basic fibroblast growth factor alter its biological activity. | eleven structural analogues of human basic fibroblast growth factor (bfgf) have been prepared by site-directed mutagenesis of a synthetic bfgf gene to examine the effect of amino acid substitutions in the three putative heparin-binding domains on fgf's biological activity. after expression in escherichia coli, the mutant proteins were purified to homogeneity by use of heparin-sepharose chromatography and analyzed for their ability to stimulate dna synthesis in human foreskin fibroblasts. recombi ... | 1991 | 2036431 |
| [isolation of vero toxin-producing escherichia coli (vtec) from imported meats]. | one hundred and four samples of various imported meats obtained from the stores and supermarkets in matsuyama-city and iyo-city from november, 1987 to october, 1988 were examined and two strains of vero toxin-producing escherichia coli were isolated: one was from beef from australia and the other was from pork from canada. by bead-elisa, it was confirmed that the strain from beef produced vero toxin 1 (vt1) and vero toxin 2 variant (vt2vh) and the strain from pork produced vt2vh. these results s ... | 1991 | 2066599 |
| the influence of anti-inflammatory therapy on bacterial clearance following intramammary escherichia coli challenge in goats. | coliform mastitis in dairy cattle frequently results in systemic disease with occasional deaths in association with endotoxic shock. systemic anti-inflammatory therapy has been used to alter the course of endotoxic shock in severe cases. use of anti-inflammatory therapy has been questioned on the basis that such treatment may compromise immune function and decrease clearance of bacteria from infected mammary glands. the purpose of this investigation was to determine whether anti-inflammatory the ... | 1991 | 2068798 |
| verotoxic escherichia coli in human disease. | verotoxin-producing e. coli (most frequently e. coli o157) have been implicated in the pathogenesis of diarrhea, hemorrhagic colitis, hemolytic uremic syndrome and thrombotic thrombocytopenic purpura. cattle, meat products, and other sources have been found to harbor these organisms. isolation of e. coli o157:h7 on macconkey-sorbitol agar is diagnostic, yet the bacteria are difficult to detect after the first week of infection. enzyme linked immunosorbent assays (elisas) can detect verotoxin in ... | 1991 | 2072218 |
| isolation of escherichia coli serotype o157:h7 and other shiga-like-toxin-producing e. coli from dairy cattle. | we examined 1,266 fecal specimens from healthy cattle during the investigations of two sporadic cases of hemolytic uremic syndrome associated with raw milk consumption and an outbreak of gastroenteritis and hemolytic uremic syndrome caused by escherichia coli serotype o157:h7. we collected specimens from heifers, calves, and adult cows on 22 farms, in a stockyard, and in a packing house. we also collected 3 raw hamburger specimens from a restaurant and 23 raw milk samples from two farms. all spe ... | 1991 | 2056066 |
| intramammary challenge with escherichia coli following immunization with a curli-producing escherichia coli. | holstein and jersey cattle were immunized with a curli-producing strain of escherichia coli (pcrl65/a012) or a noncurli-producing strain (puc18/hb101) to determine differences in resistance to establishment of experimental intramammary infection. cows (n = 6 per group) were immunized at 14 d prior to drying off, 7 d of involution, and at calving with 3 x 10(10) e. coli in freund's incomplete adjuvant. at 30 d of lactation, one mammary quarter of each cow was infused with a wild strain of e. coli ... | 1991 | 2071713 |
| fibroblast adhesion to recombinant tropoelastin expressed as a protein a-fusion protein. | a bovine tropoelastin cdna encoding exons 15-36 that includes the elastin-receptor binding site was expressed in escherichia coli as a fusion protein with protein a from staphylococcus aureus. after isolation of the fusion protein by affinity chromatography on ig-sepharose, the tropoelastin domain was separated from plasmid-pr1t2t-encoded protein a (protein a') by cnbr cleavage. cell-adhesion assays demonstrated specific adhesion to the recombinant tropoelastin. furthermore, the data indicate th ... | 1991 | 1996952 |
| 16s ribosomal dna amplification for phylogenetic study. | a set of oligonucleotide primers capable of initiating enzymatic amplification (polymerase chain reaction) on a phylogenetically and taxonomically wide range of bacteria is described along with methods for their use and examples. one pair of primers is capable of amplifying nearly full-length 16s ribosomal dna (rdna) from many bacterial genera; the additional primers are useful for various exceptional sequences. methods for purification of amplified material, direct sequencing, cloning, sequenci ... | 1991 | 1987160 |
| the signal for golgi retention of bovine beta 1,4-galactosyltransferase is in the transmembrane domain. | the expression and localization of bovine beta 1,4-galactosyltransferase (gal t) has been studied in mammalian cells transfected with gal t cdna constructs, and the role of the amino-terminal domains of gal t in golgi localization examined. here we demonstrate that the transmembrane (signal/anchor) domain of bovine gal t contains a positive golgi retention signal. bovine gal t was characterized in transfected cells with anti-bovine gal t antibodies, affinity-purified from a rabbit antiserum usin ... | 1992 | 1637374 |
| the effect of cyclosporin a on cationized bovine serum albumin-induced nephropathy in nzw rabbits. | the effect of cyclosporin a (cya) was studied on the morphology and protein excretion of a rabbit chronic serum sickness nephritis using cationized bovine serum albumin (cbsa). one group of rabbits was given intravenous (i.v.) immunizing doses of cbsa and escherichia coli endotoxin. one week later, these animals began a 6-week i.v. injection schedule of cbsa only. a second group followed the same injection protocol, but was given intramuscular (i.m.) cya for 3 days prior to the immunizing dose o ... | 1992 | 1625057 |
| meningitis in neonatal calves: 32 cases (1983-1990). | case records of 32 neonatal calves with the antemortem diagnosis of meningitis were reviewed. mean age at admission was 6 days (range, 11 hours to 30 days), and the most common concurrent clinical problem was diarrhea (16/32). twenty-seven of the calves were available for necropsy. at postmortem, there was evidence of septicemia in 22 (81%) of these calves. escherichia coli was the organism most frequently isolated (11/16; 69%) from the cns. the major clinical signs of cns disturbance observed o ... | 1992 | 1644635 |
| recombinant hiv-1 nucleocapsid protein p15 produced as a fusion protein with glutathione s-transferase in escherichia coli mediates dimerization and enhances reverse transcription of retroviral rna. | human immunodeficiency virus 1 (hiv-1) nucleocapsid protein p15 was produced as a fusion protein with glutathione s-transferase (gst) in escherichia coli. rapid purification of gst::p15 in an active form by one-step glutathione-agarose chromatography was accomplished in the presence of an antioxidant. recombinant p15 fused to gst was shown to stimulate the dimerization of viral rna. hiv-1 reverse transcriptase-catalyzed in vitro synthesis of minus-strand cdna from synthetic human trna(lys3uuu) a ... | 1992 | 1280240 |
| recombinant polypeptide from the gp48 region of the bovine viral diarrhea virus (bvdv) detects serum antibodies in vaccinated and infected cattle. | to characterize the immune response of cattle to bovine viral diarrhea virus (bvdv) glycoprotein gp48, we have produced a large amount of recombinant glutathione-s-transferase-gp48 (gst-gp48) fusion protein in escherichia coli. antibodies to gp48 were present in cattle vaccinated with killed or modified-live virus vaccination, or following natural infection. these results were in agreement with results of serum neutralization (sn) test which detected gp53 of bvdv. | 1992 | 1333674 |