Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| effects of the lipopolysaccharide-protein complex and crude capsular antigens of pasteurella multocida serotype a on antibody responses and delayed type hypersensitivity responses in the chicken. | the effects of the lipopolysaccharide-protein complex (lps) and crude capsular antigen (cca) prepared from pasteurella multocida serotype a isolated from a duck in the philippines, on antibody responses to sheep red blood cells (srbc) and brucella abortus (ba) and delayed type hypersensitivity (dth) responses to bovine serum albumin (bsa) in the chickens were studied. chickens injected subcutaneously with lps and cca at 1 and 2 weeks of age and immunized intravenously with the mixed antigens of ... | 1999 | 10379953 |
| identification of an is711 element interrupting the wboa gene of brucella abortus vaccine strain rb51 and a pcr assay to distinguish strain rb51 from other brucella species and strains. | brucella abortus vaccine strain rb51 is a natural stable attenuated rough mutant derived from the virulent strain 2308. the genetic mutations that are responsible for the roughness and the attenuation of strain rb51 have not been identified until now. also, except for an assay based on pulsed-field gel electrophoresis, no other simple method to differentiate strain rb51 from its parent strain 2308 is available. in the present study, we demonstrate that the wboa gene encoding a glycosyltransferas ... | 1999 | 10473532 |
| immunohistochemical detection of brucella abortus antigens in tissues from aborted bovine fetuses using a commercially available polyclonal antibody. | a commercially available polyclonal antibody and an avidin-biotin-peroxidase immunohistochemical technique were used to detect brucella abortus antigens in formalin-fixed, paraffin-embedded tissues of lung and liver from 20 aborted bovine fetuses. thirteen fetuses were obtained from farms with a previous history of brucellosis, and 7 were collected from farms without a history of brucellosis. among the 13 aborted bovine fetuses obtained from farms with a history of brucellosis, immunoreactivity ... | 1998 | 9526855 |
| efficacy of elisa compared to conventional tests (rbpt and cft) for the diagnosis of brucella melitensis infection in sheep. | an indirect elisa (ielisa) using a b. abortus smooth lipopolysaccharide (s-lps) as coating antigen and a polyclonal anti-sheep igg peroxydase-labeled serum as conjugate was developed. the ielisa was assessed in comparison to the food and agriculture organisation/international atomic energy agency (fao/iaea) standard bovine elisa kit (iaea kit) using an anti-bovine igg conjugate that cross-reacts with sheep antibodies, and with complement fixation test (cft) and rose bengal plate test (rbpt). the ... | 1998 | 9874104 |
| field study of vaccination of cattle with brucella abortus strains rb51 and 19 under high and low disease prevalence. | to assess humoral and protective immunity in cattle vaccinated by 12 months with brucella abortus vaccine strains rb51 and 19 under field conditions of high and low brucellosis prevalence. | 1998 | 9706206 |
| is brucella abortus a facultative intracellular pathogen with mitochondria-like activity? | brucella abortus is the agent of bovine brucellosis, a zoonotic disease of worldwide importance. in latently infected humans and animals, acute disease may recur under conditions that decrease the host resistance. this bacterium is considered to be a facultative intracellular pathogen. however, its pathogenic attributes appear reduced in comparison with other gram-negative pathogens. it has been recognized that b. abortus and other brucella species reach their intracellular location inside the r ... | 1998 | 9881835 |
| simultaneous identification of antibodies to brucella abortus and staphylococcus aureus in milk samples by flow cytometry. | two flow cytometric assays are described herein. the single cytometric test (sct) detects antibodies to either brucella abortus or staphylococcus aureus in the serum or milk of a cow or water buffalo. the double cytometric test (dct) detects both anti-b. abortus and anti-s. aureus antibodies concurrently. in the sct, the sample to be tested is incubated in succession with the antigen (either b. abortus or s. aureus) and the proper secondary antiserum (fluorescein isothiocyanate-labelled rabbit a ... | 1998 | 9508316 |
| experimental use of a dot-blot assay to measure serologic responses of cattle vaccinated with brucella abortus strain rb51. | brucella abortus strain rb51 was recently approved as an official brucellosis calfhood vaccine for cattle by the animal and plant health inspection service branch of the united states department of agriculture. currently available serologic surveillance tests for b. abortus do not detect seroconversion following srb51 vaccination. the purpose of this study was to evaluate a dot-blot assay using gamma-irradiated strain rb51 bacteria for its specificity and sensitivity to detect antibody responses ... | 1997 | 9376424 |
| variations in the prevalence of antibody to brucella infection in cattle by farm, area and district in kenya. | variations in the sero-prevalence of antibody to brucella infection by cow, farm and area factors were investigated for three contrasting districts in kenya: samburu, an arid and pastoral area: kiambu, a tropical highland area; and kilifi, a typical tropical coastal area. cattle were selected by a two-stage cluster sampling procedure and visited once between august 1991 and 1992. schall's algorithm, a statistical model suitable for multi-level analysis was used. using this model, older age, free ... | 1997 | 9042033 |
| safety and immunogenicity of brucella abortus strain rb51 vaccine in pregnant cattle. | to determine the safety and immunogenicity of brucella abortus strain rb51 as a vaccine in pregnant cattle. | 1997 | 9140553 |
| a homogeneous fluorescence polarization assay for detection of antibody to brucella abortus. | a homogeneous fluorescence polarization (fp) assay (fpa) was developed for detection of antibody in bovine sera to brucella abortus. the assay used o-polysaccharide prepared from b. abortus lipopolysaccharide in the molecular weight range of 20-30 kda which was conjugated with fluorescein isothiocyanate and used as a tracer. fluorescence polarization was measured with a fpm-1 fluorescence polarization analyzer. sample (20 microliters) was added to 2.0 ml of diluent buffer at ambient temperature. ... | 1996 | 8814332 |
| development and validation of an indirect enzyme immunoassay for detection of antibody to brucella abortus in milk. | an indirect enzyme immunoassay for detection of antibody to brucella abortus in bovine milk was developed and validated using 6238 milk samples from canadian herds (brucellosis free) and 202 samples from herds infected with b. abortus (from argentina and chile). the assay utilized lipopolysaccharide as the antigen, immobilized on the polystyrene matrix, whole milk to test and a mouse monoclonal antibody, specific for an epitope of bovine igg1, conjugated with horseradish peroxidase. the sensitiv ... | 1996 | 8914260 |
| lymphocyte proliferation in response to brucella abortus rb51 and 2308 proteins in rb51-vaccinated or 2308-infected cattle. | cattle vaccinated with brucella abortus strain rb51 (srb51) or infected with strain 2308 (s2308) had lymph node lymphocytes which proliferated most when incubated with 32-, 27-, 18-, or <18-kda proteins of either srb51 or s2308. some s2308-infected cattle but no srb51-vaccinated cattle had lymphocytes which proliferated in response to 80- and 49-kda proteins of srb51 and s2308. these results suggest that cattle vaccinated with srb51 or infected with s2308 have lymphocytes which proliferate in re ... | 1996 | 8641749 |
| brucella abortus differs in the multiplication within bovine chorioallantoic membrane explants from early and late gestation. | the ability of brucella to infect and grow within extraplacentomal chorioallantoic explants (cams) derived from early and late gestational cattle was compared. following inoculation of cams with equal numbers of strain 2308 b. abortus, the infectivity was approximately the same in cams from both ages, however, bacterial replication was significantly greater in late gestational cams than in early gestational cams. co-culture of both early and late gestation cams or culture of both types of cams i ... | 1996 | 8654046 |
| humoral immune response against lipopolysaccharide and cytoplasmic proteins of brucella abortus in cattle vaccinated with b. abortus s19 or experimentally infected with yersinia enterocolitica serotype 0:9. | the humoral immune responses against three different antigens of brucella abortus were monitored by enzyme-linked immunosorbent assay in cattle vaccinated with b. abortus s19 or experimentally infected with yersinia enterocolitica serotype 0:9. immunoglobulin g (igg) and igm responses against (i) b. abortus lipopolysaccharide (lps), (ii) total cytoplasmic proteins depleted of lps (lps-free cyt), and (iii) b. abortus 18-kda cytoplasmic protein were measured. vaccinated animals and yersinia-infect ... | 1996 | 8807216 |
| seroprevalences of brucellosis, q-fever and toxoplasmosis in slaughter livestock in trinidad. | serum samples obtained from livestock (cattle, chickens, pigs, sheep, goats and water buffaloes) slaughtered at various slaughter houses in trinidad were screened for agglutinins to three zoonosis causing pathogens. of a total of 751 sera tested, 2 (0.3%) originating from chickens were positive for brucella abortus agglutinins using the rose bengal test (rbt), but both were negative by the tube serum agglutination test (sat). thirty-six (4.8%) of 749 sera were positive for coxiella burnetii aggl ... | 1996 | 8881416 |
| serologic responses of brucella abortus strain 19 calfhood-vaccinated cattle following adult vaccination with strain rb51. | this study was designed to determine if brucella abortus strain rb51, which expresses small amounts of the lipopolysaccharide o side chain, would cause positive responses on brucellosis serologic surveillance tests when given to adult cattle that were vaccinated as calves with b. abortus strain 19. cattle vaccinated as adults with strain rb51 that had been vaccinated as calves with strain 19 (n = 40) had significantly greater antibody titers (p < 0.05) against strain rb51 at 4 and 8 weeks postva ... | 1996 | 8953530 |
| evidence for genetic control of vaccine-induced antibody responses in cattle. | the purpose of our study was to identify evidence for genetic control of immune responses in cattle. to address this question, we evaluated the variation of antibody responses induced by vaccination with brucella abortus strain 19, a live attenuated bacterial vaccine, in large half-sibling families. the data were analyzed using a parametric statistical model that incorporated the effects of sire, bovine major histocompatibility complex (bola) types and parameters related to the experimental desi ... | 1996 | 9157685 |
| intracellular survival of brucella abortus, mycobacterium bovis bcg, salmonella dublin, and salmonella typhimurium in macrophages from cattle genetically resistant to brucella abortus. | peripheral blood monocyte-derived macrophages were obtained from a herd of cows selected, bred, and confirmed as resistant or susceptible by in vivo challenge of brucella abortus strain 2308. the ability to control in vitro intracellular bacterial replication of b. abortus strain 2308, mycobacterium bovis bacillus calmette-guerin (bcg) montreal strain 9003, salmonella dublin strain 5631, and salmonella typhimurium strain 14028 was evaluated in a bactericidal assay. the macrophages from resistant ... | 1996 | 9157686 |
| serological response of cattle to brucella allergen after repeated intradermal applications of this allergen. | a study was conducted to determine whether an allergen that has been prepared from a mucoid strain of brucella abortus triggers a serum antibody response that interferes with the interpretation of serologic tests results. fifteen cattle seronegative for brucella antigen were tested with the sdth test several times. blood samples were collected weekly and tested with the serum agglutination test and complement fixation test. results show that some cattle tested seronegative after each of the sdth ... | 1996 | 8701573 |
| infection of cattle with brucella abortus biovar 1 isolated from a bison in wood buffalo national park. | an experiment was conducted to determine if cattle could be infected with a strain of brucella abortus biovar 1 isolated from a bison in wood buffalo national park. three pregnant cows inoculated conjunctivally with 5.7 x 10(8) cfu of the bacterium, and their subsequent calves, showed seroconversion on standard serological tests for bovine brucellosis, and large numbers of the bacterium were isolated from numerous tissues at necropsy. a 4th cow that was moved into the pen that previously contain ... | 1996 | 8809394 |
| single-step pcr for detection of brucella spp. from blood and milk of infected animals. | a versatile method for the extraction of brucella dna and pcr are presented as reliable tools for the detection of brucella spp. from body fluids of infected animals. two oligonucleotides homologous to regions of the gene encoding for an outer membrane protein (omp-2) were designed to detect the pathogen from milk and/or blood of infected goats, bovines, and human patients. the sensitivity of our test and its ability to detect the pathogen in samples from the field reveal a promising advance in ... | 1995 | 8586678 |
| [use of the b19 strain in the prevention of bovine brucellosis in northern cameroon. study of effect of the dose on seroconversion rate and duration in female zebu]. | four herds of zebus from northern cameroon totalling 136 animals were vaccinated subcutaneously with the following doses of brucella abortus strain 19: 5 x 10(9) colony-forming units (cfu), 10(9) cfu, 5 x 10(8) cfu and 10(7) cfu. twenty-eight days after vaccination, the following seroconversion rates were observed respectively: 97.4, 96.2 84.2 and 73.3%. of the 52 animals which could be tested subsequently including 39 over one year old on the vaccination day, only one showed antibodies 6 months ... | 1995 | 7569229 |
| enhancement of the brucella amos pcr assay for differentiation of brucella abortus vaccine strains s19 and rb51. | because the brucellosis eradication program uses slaughter and quarantine as control measures, it would benefit from faster methods of bacterial identification. distinguishing vaccine strains from strains that cause infections among vaccinated herds in the field is essential. to accomplish this, our pcr-based, species-specific assay (b. j. bricker and s. m. halling, j. clin. microbiol. 32:2660-2666, 1994) was updated to identify brucella abortus vaccine strains s19 and rb51. three new oligonucle ... | 1995 | 7650203 |
| [the epidemiological characteristics of brucellosis in the region of the northern caucasus]. | at present, the northern caucus region is the most unfavourable brucellosis area of the russian federation where 43.5% of the total russian incidence of this infection was recorded in 1993. over the past years (1988-1993) there was a highly unsteady-state improvement of the epidemiological situation, however, the rates of human morbidity with this infection greatly lag behind those in russia. the active foci of small cattle brucellosis as the leading source of human contamination with brucellosi ... | 1995 | 8587517 |
| contradictory roles for antibody and complement in the interaction of brucella abortus with its host. | the ability of serum complement to kill bacteria has been linked to host resistance to gram-negative bacteria. a mechanism for killing extracellular organisms during early invasion, following release from infected phagocytic cells, or during bacteremia would contribute to a host's ability to resist disease. in fact, the ability of serum complement to kill bacteria has been linked to disease resistance. brucella abortus are gram-negative intracellular pathogens. resistance to these bacteria invol ... | 1995 | 8845060 |
| non-specific seroreactions against brucella abortus in ruminants in new zealand and the presence of yersinia enterocolitica 0:9. | the level of non-specific reactions found in the brucellosis serology of ruminants in new zealand was very low until july 1992. this changed when, in an export consignment of 1071 deer, 35% reacted in the brucella abortus tube agglutination test with titres varying from 50 to 200 iu. the reactors were also positive in the rose-bengal agglutination test and most of them reacted in the complement fixation test with titres varying from 10 to 80 iu. yersinia enterocolitica 0:9 was later isolated fro ... | 1995 | 16031844 |
| the use of divalent cation chelating agents (edta/egta) to reduce non-specific serum protein interaction in enzyme immunoassay. | an indirect enzyme immunoassay (elisa) for detection of bovine antibody to brucella abortus was modified by the addition of divalent chelating agents to the serum diluent. this addition resulted in an increase in specificity from 96.0% in the regular assay to 99.4% in the modified procedure. of the 15,715 sera initially tested by the indirect elisa, 691 that had given positive reactions were selected for retesting in the indirect elisa with edta/egta added. the buffered plate antigen test (bpat) ... | 1994 | 7701780 |
| [epidemiology of bovine brucellosis in mali: serologic investigation and initial isolation of strains of brucella abortus]. | the authors report the results of an epidemiological survey of bovine brucellosis in mali, based on a relatively representative sample of 1,000 serum samples from 236 herds. the prevalence of infection in the herds, established by indirect enzyme-linked immunosorbent assay (elisa) was 53% +/- 6.4. the proportion of animals infected was 23.3% +/- 2.5, falling to 22% when compared with the basic serum pool of 9,466 samples. this rate was relatively high in stationary herds in the semi-arid, sub-hu ... | 1994 | 7949352 |
| comparison of particle concentration fluorescence immunoassay to card and complement fixation tests using isolation of brucella abortus as the standard. | serologic test data and bacteriologic culture results from 816 cattle were evaluated. brucella abortus (field strain or strain 19) was isolated from 27.3% of the cows. results of the card test, particle concentration fluorescence immunoassay (pcfia), and complement fixation (cf) test were compared. antibody titers were directly associated with b. abortus isolation, with the majority (64.7%) of field strain isolations having a pcfia value of < or = 0.300 and a cf test result of at least 1+ at a 1 ... | 1994 | 8068749 |
| inhibition of complement-mediated killing of brucella abortus by fluid-phase immunoglobulins. | bovine immunoglobulin preparations from normal serum and from sera containing antibodies against brucella abortus interfered with the brucellacidal action of bovine serum, whereas unfractionated normal serum and antisera were not inhibitory. the inhibitory property of immunoglobulin appeared to be attributable to some anticomplementary property because it also interfered with serum-mediated hemolysis of antibody-coated erythrocytes. the supernatant phase obtained after ultracentrifugation of bov ... | 1994 | 7944019 |
| the brucella abortus vaccine strain b19 carries a deletion in the erythritol catabolic genes. | brucella abortus b19, an avirulent strain obtained by spontaneous mutation, is used worldwide as a vaccine for the control of bovine brucellosis. b19 differs from other b. abortus strains in its sensitivity to erythritol. we took advantage of a previously obtained erythritol sensitive tn5 insertion mutant of b. abortus 2308 to clone the chromosomal region containing erythritol catabolic genes from this representative pathogenic strain and from the vaccine strain b19. physical mapping with restri ... | 1994 | 7926690 |
| mechanisms of binding of brucella abortus to mononuclear phagocytes from cows naturally resistant or susceptible to brucellosis. | during the course of bovine brucellosis, brucella abortus adheres to and infects cells of the mononuclear phagocyte system. potential mechanisms of binding, as measured by numbers of phagocytosed bacteria, were studied in two populations of cattle genetically resistant (r) or susceptible (s) to infection with b. abortus. live b. abortus gained entry into cultured bovine macrophages without organism-specific opsonization. bacterial entry into macrophages from r was inhibited by the peptide rgds, ... | 1994 | 7941309 |
| immune responses to superoxide dismutase and synthetic peptides of superoxide dismutase in cattle vaccinated with brucella abortus strain 19 or rb51. | antibody and lymph node cell-mediated immune responses to recombinant brucella abortus strain 19 cu-zn superoxide dismutase (rsod) and to three synthetic strain 19 cu-zn sod peptides were measured during 2 to 12 weeks following vaccination of cattle with b. abortus strain 19 or rb51. cattle vaccinated with strain 19 or rb51 did not produce antibody to rsod and to the sod peptides. lymph node cells from cattle vaccinated with strain 19, but not with strain rb51, proliferated when incubated with e ... | 1994 | 7801538 |
| brucella abortus serology in cattle naturally infected with escherichia coli o157.h7. | 1994 | 7831743 | |
| cloning, characterization and construction of htra and htra-like mutants of brucella abortus and their survival in balb/c mice. | a genomic library of brucella abortus s2308 was screened for expression of recombinant proteins recognized by sera from mice and from cattle infected with b. abortus. a positive clone, ba1, expressing a 50 kda peptide was recognized by both sera. plasmid pba1, isolated from ba1, was shown by restriction enzyme digestion to possess a 1.9 kb insert. the nucleotide sequence of the pba1 insert revealed an open reading frame with of 1539 bases with a coding capacity of 513 amino acids and a predicted ... | 1994 | 7861951 |
| application of enzyme-linked immunosorbent assay for epidemiological studies of diseases of livestock in the tropics of mexico. | this study was conducted at the centre for research, teaching and extension in tropical livestock (centro de investigación, enseñanza y extensión en ganadería tropical) of the faculty of veterinary medicine of the national autonomous university of mexico. during the latter part of 1986 and throughout 1988 and 1989, the herd of holstein x zebu cattle at the university was tested for igg antibodies to twenty-one viral, bacterial, rickettsial and parasitic agents. antigens prepared from twenty infe ... | 1993 | 8219327 |
| immune responses and protection against infection and abortion in cattle experimentally vaccinated with mutant strains of brucella abortus. | twenty-four 10-month-old polled hereford heifers were inoculated sc with live cells of one of the following strains of brucella abortus: s19 delta 31k (n = 4), s19 delta sod (n = 4), rb51 (n = 4), and strain 19 (n = 6); controls (n = 6) were given saline solution. heifers given the deletion mutants s19 delta 31k and s19 delta sod, and those given strain 19 developed antibody responses to b abortus and cutaneous reactions to brucellin. heifers given strain rb51 did not develop antibodies that rea ... | 1993 | 8250382 |
| [latency of brucella abortus causes problems in oriented control: a review]. | this review on brucella abortus in cattle covers the pathogenesis, the epidemiology and the diagnostics of brucellosis. emphasis is given to the presence of latent infections in young stock. calves infected by b. abortus in utero or after ingestion of infected milk may acquire a persistent infection. these animals might present a significant problem in brucellosis control and eradication schemes, since they are difficult to detect by the usual serological tests as they remain negative until near ... | 1993 | 8256248 |
| brucellosis: imported and laboratory-acquired cases, and an overview of treatment trials. | following the successful eradication of brucella abortus infection in cattle, human brucellosis in england and wales has become an uncommon imported disease. culture of the organism presents a major laboratory hazard, and difficulties in identification may occur using a biochemical test-strip method. an overview of recent treatment trials of brucellosis indicates that regimens combining streptomycin and doxycycline are associated with a higher success rate (judged by the frequency of treatment f ... | 1993 | 8337710 |
| [evaluation of epidemiologic surveillance of human brucellosis-- occupational disease overcome in the midst of animals]. | national eradication programme of bovine brucellosis in poland has reduced prevalence below 0.5%. the last cow-abortion incidence caused by brucella abortus bovis was recognized in 1982. the registered cases of human brucellosis (610 during 1986-1992) represent chronic occupational infections in man acquired earlier, when bovine brucellosis was common in poland. actually, some of victims show no serological evidence of infection. present small risk of infection permits to stop or to limit (only ... | 1993 | 8171199 |
| a serological survey of bovine brucellosis in the molopo district of bophuthatswana. | a serological investigation was carried out in the molopo district of bophuthatswana to determine the prevalence of brucellosis titre in heifers and cows (n = 9,675) over 24 months of age, which were grazing on communal tribal and privately owned land. the rose bengal, micro serum agglutination and complement fixation (cft) tests were used. two hundred and two (2.1%) animals tested positive with a cft titre of 30 (iu ml-1) or higher. factors that could have contributed to the relatively low prev ... | 1993 | 8176693 |
| comparative analysis of using mtt and xtt in colorimetric assays for quantitating bovine neutrophil bactericidal activity. | two different tetrazolium compounds were compared for use in a colorimetric assay for quantitating bovine neutrophil bactericidal activity against staphylococcus aureus, escherichia coli, listeria monocytogenes, and brucella abortus. the tetrazolium compounds tested included 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (mtt) and sodium 3,3'-[1[(phenylamino)carbonyl]-3,4- tetrazolium]-bis(4-methoxy-6-nitro) benzene sulfonic acid hydrate (xtt). the mtt and xtt colorimetric bacteric ... | 1993 | 8423367 |
| prevention of pathophysiologic and immunomodulatory effects of gastrointestinal nematodiasis in calves by use of strategic anthelmintic treatments. | effects of strategic anthelmintic treatment on pathophysiologic and immunomologic changes induced by infection with ostertagia ostertagi and cooperia oncophora were studied in 2 groups, of 12 calves each: an infected group, inoculated with 200,000 mixed o ostertagi and c oncophora third-stage larvae (l3) on day 1; and an infected-treated group, similarly inoculated, but treated with ivermectin at 9 and 33 days. all calves were also inoculated at 12 weeks with brucella abortus vaccine, at 13 week ... | 1993 | 8116936 |
| control of brucellosis in kuwait by vaccination of cattle, sheep and goats with brucella abortus strain 19 or brucella melitensis strain rev. 1. | in kuwait, approximately 12,000 dairy cows were vaccinated with a reduced dose of 3 x 10(9) brucella abortus strain 19 and approximately 350,000 sexually mature sheep and goats with a reduced dose of 10(7) b. melitensis strain rev. 1. using the criteria of prevaccinal and postvaccinal incidences of antibodies, abortions, and human cases of brucellosis, the programme was very successful. widespread vaccination of adult animals is the most effective method of controlling brucellosis among cattle, ... | 1992 | 1306918 |
| childhood brucellosis in southwestern saudi arabia: a 5-year experience. | one-hundred-and-fifty-seven children admitted with brucellosis at abha, saudi arabia, were studied prospectively. ninety-two per cent gave a history of animal contact, usually with sheep or goats, or ingesting raw milk, milk products, or raw liver. three-quarters of the patients had an acute or subacute presentation with diverse symptomatology: fever (100 per cent), malaise (91 per cent), anorexia (68 per cent), cough (20 per cent), abdominal symptoms (20 per cent), arthralgia (25 per cent). hep ... | 1992 | 1527811 |
| antigens of brucella abortus s19 immunodominant for bovine lymphocytes as identified by one- and two-dimensional cellular immunoblotting. | cellular immune responses are influential for protection against intracellular bacteria such as brucellae. therefore, identification of brucella abortus antigens that activate primed bovine lymphocytes is fundamental for discerning the breadth of cellular response in bovine brucellosis. potentially antigenic components of b. abortus s19 were isolated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by nitrocellulose blotting. specific one-dimensional blot segments induced pr ... | 1992 | 1587614 |
| interaction of bovine chorioallantoic membrane explants with three strains of brucella abortus. | chorioallantoic membrane (cam) explants were used to determine the in vitro growth and cytotoxic potential of 3 strains of brucella abortus. bovine cam explants were inoculated with 2 x 10(7) colony-forming units of the pathogenic strain 2308, attenuated strain 19, or the rough strain rb51 of b abortus. after inoculation, the explants were harvested and examined at 2 or 4 hours, 12 or 14 hours, and 24 or 26 hours of incubation. bacterial growth associated with each explant was determined by coun ... | 1992 | 1595961 |
| antibody response to brucella outer membrane proteins in bovine brucellosis: immunoblot analysis and competitive enzyme-linked immunosorbent assay using monoclonal antibodies. | sera from brucella-infected bovines were analyzed by immunoblotting by using sonicated cell extracts of b. melitensis or b. abortus and a competitive enzyme-linked immunosorbent assay (elisa) with monoclonal antibodies against outer membrane proteins (omps) with molecular masses of 10, 16.5, 19, 25 to 27, 36 to 38, and 89 kda. antibody responses against omps were compared with antibody responses against smooth lipopolysaccharide. immunoblot analysis indicated that the antibody response in infect ... | 1992 | 1452700 |
| the effects of adjuvants on immune responses in cattle injected with a brucella abortus soluble antigen. | five different adjuvants were examined for potentiation of humoral and cell-mediated immune (cmi) responses in cattle to a brucella abortus soluble antigen (basa). two separate experiments were performed involving a total of 64 steers, divided among six groups (experiment 1) and 9 groups (experiment 2). the adjuvants used were: muramyl dipeptide, freund's incomplete adjuvant, dimethyl-dioctadecyl ammonium bromide (dda), bordetella pertussis and propionibacterium acnes. in each experiment, three ... | 1991 | 1835213 |
| differentiation by western blotting of immune responses of cattle vaccinated with brucella abortus strain 19 or infected experimentally or naturally with virulent brucella abortus. | brucella abortus strain 19 salt-extractable proteins fractionated by differential ammonium sulfate precipitation were used in a western blotting method to detect bovine immunoglobulin g antibodies to b. abortus. sera from infected cattle and from cattle vaccinated with strain 19 and subsequently exposed to virulent b. abortus bound to a common group of antigens ranging in molecular weights from 31,000 to 45,000 daltons. immunoglobulin g antibodies in sera from the latter group in addition also b ... | 1991 | 1904667 |
| effects of stage of gestation and breed on bovine responses to vaccination with brucella abortus strain 19. | eighty-eight cattle were injected sc with 2.5 x 10(8) viable cells of brucella abortus strain 19. all but 1 heifer became seropositive on the basis of the results of 7 brucellosis tests, and the proportion positive decreased with time. the proportion of cattle that were seropositive during a 20- to 67-week period after vaccination was as follows, in decreasing order: hemolysis-in-gel, 59%; buffered-acid plate antigen, 39%; elisa, 16%; card, 10%; rivanol, 8%; cold complement-fixation, 7%; and aut ... | 1991 | 1769876 |
| evaluation of serologic and cellular immune responses of cattle to a nonlipopolysaccharide antigen from brucella abortus. | cows naturally infected with brucella abortus developed antibody (ab) responses to a nonlipopolysaccharide antigen (nla) purified from b abortus strain 1119-3. sera from strain 19-vaccinated cows did not have detectable amounts of ab. weak lymphoproliferative responses to nla were observed in blood mononuclear cell suspensions obtained from infected cows. there was no evidence of nla-specific lymphoproliferation in cell suspensions from healthy cows. nonlipopolysaccharide antigen binding to bovi ... | 1990 | 2105681 |
| enzyme immunoassay using mouse monoclonal anti-bovine antibodies for the detection of brucella abortus antibodies in cow milk. | individual milk samples and artificially constructed tank milk samples from cows with naturally occurring brucellosis were examined by the enzyme-linked immunosorbent assay (elisa) using sonicated b. abortus s-99 antigen, and mouse monoclonal anti-bovine igm, iga, and igg1 conjugates. elisa results were compared with the results of the milk ring test using either 1 ml milk (mrt-1) or 8 ml milk (mrt-8). the elisa using mouse monoclonal anti-bovine igg1 conjugate was sensitive and specific. in tes ... | 1990 | 2127977 |
| brucella abortus infection in 14 farm dogs. | fourteen dogs were obtained from 10 farms with brucella-infected cattle and were studied for periods ranging from 2 to 81 days. at necropsy, brucella abortus biovar 4 was isolated from all 14 dogs. mandibular, medial retropharyngeal, tracheobronchial, and mesenteric lymph nodes yielded the highest rate of recovery. urogenital infection with active shedding was seen in a single aged bitch. fecal samples (291 from 13 dogs) were b abortus culture negative. ten dogs monitored serologically over time ... | 1990 | 2107157 |
| bovine t-lymphocyte lines reactive with brucella abortus. | bovine t-cell lines reactive with brucella abortus were established by repeated stimulation with b abortus and mitomycin c-treated autologous antigen-presenting cells. representative results were obtained, using 33 cell lines from 14 cows. cultures responded to the virulent laboratory strain 2308, the vaccine strain 19, and the rough mutant strain rb51 in thymidine-incorporation assays. the cells in these cultures required antigen-presenting cells for their response to b abortus. autologous anti ... | 1990 | 2109554 |
| immunopathology of experimental brucella abortus strain 19 infection of the genitalia of bulls. | antibody responses in serum and semen, and immunoglobulin containing cell (icc) populations in the genitalia of bulls were compared after inoculating brucella abortus strain 19 into the seminal vesicles of two bulls (isv route) and into testes in two other bulls (it route). bulls seroconverted as early as 1 week post-infection (pi). peak serum titres as determined by the serum agglutination test (sat), complement fixation test (cft) and elisa occurred at pi weeks 3, 4 and 5 respectively. highest ... | 1990 | 2111057 |
| a survey of brucellosis and tuberculosis in bison in and around wood buffalo national park, canada. | examinations of complete or partial remains of 72 bison found dead in and around wood buffalo national park, canada, revealed evidence of brucellosis in 18 (25%) and tuberculosis in 15 (21%), with a combined prevalence of 42%. urease-positive and ureasenegative strains of brucella abortus biovar 1, and strains of biovar 2, were isolated from tissues of bison, including synovium and exudate from severe arthritic lesions. mycobacterium bovis was isolated from a range of granulomatous lesions that ... | 1990 | 17423532 |
| sepracell-mn for mononuclear cell separation from cattle: effect on mitogen-and antigen-induced lymphocyte blastogenesis. | mononuclear leukocytes (mnc) were separated from heparinized and edta-treated whole bovine blood by centrifugation after mixing with a commercial colloidal silica preparation (sepracell-mn (s-mn]. cell yields and lymphocyte blast transformation (lbt) to pokeweed mitogen (pwm), phytohaemagglutinin (pha), concanavalin a (con a), and brucella abortus antigens were tested against mnc obtained from heparinized whole blood using ficoll-hypaque (fh). separation with s-mn was more rapid and less labor i ... | 1989 | 2557707 |
| macrophage function in mammary glands of brucella abortus-infected cows and cows that resisted infection after inoculation of brucella abortus. | nonvaccinated pregnant cows were segregated retrospectively into 2 groups following inoculation with brucella abortus strain 2308. one group resisted infection (resistant cows) and the other group developed active infections (susceptible cows) and subsequently aborted. mammary gland macrophages collected from the 2 groups of cows were compared, using in vitro functional assays. in a chemiluminescence assay, mammary gland macrophages from resistant cows produced significantly (p = 0.014) higher o ... | 1989 | 2496628 |
| effects of in vitro and in vivo administration of recombinant bovine interferon-gamma on bovine neutrophil responses to brucella abortus. | the effects of in vitro and in vivo treatment of bovine polymorphonuclear leukocytes with recombinant bovine interferon-gamma on in vitro bovine polymorphonuclear leukocyte functions and the survival of brucella abortus were determined. activation of neutrophils in vitro with interferon-gamma resulted in enhanced production of o2- and myelopeoroxidase-h2o2-halide activity by neutrophils in the presence of b. abortus. the improved iodination responses were correlated with an enhanced ability to p ... | 1989 | 2539694 |
| inflammatory cell and immune function in merino sheep with chronic dermatophilosis. | components of inflammatory and immunological responses were compared in 17 merino sheep with chronic dermatophilosis (group 1) and 15 merino sheep that had recovered from the disease (group 2). the functions studied included: (i) total and differential white cell counts; (ii) phagocytic function and intracellular killing by neutrophils; (iii) humoral immune response to t-dependent and t-independent antigens and to dermatophilus congolensis. (iv) lymphocyte blastogenic responses to phytohaemagglu ... | 1989 | 2623798 |
| bovine monoclonal antibody specific for brucella abortus lipopolysaccharide. | the development of a bovine monoclonal antibody against brucella abortus smooth lipopolysaccharide (bm-8) by interspecies fusion of bovine peripheral lymphocytes from an immunized cow and a murine plasmacytoma cell line is described. the twice cloned cell line secreted bovine igg1 subclass antibody. ascites fluid was prepared in pristane treated nu/nu mice by intraperitoneal injection. the pooled ascites fluid was purified by affinity chromatography and the functions of the antibody assessed in ... | 1989 | 2508298 |
| uptake and excretion of brucella abortus in tissues of the face fly (musca autumnalis). | to determine their capacity to host brucella abortus, face flies were examined 1 to 120 hours after feeding on broth containing bacteria and bovine erythrocytes. brucella abortus was cultured in large numbers from whole flies for 12 hours after feeding, but not after 72 hours. histologic analysis showed that brucellae were rapidly taken into the midgut, sequestered from erythrocytes, transiently stored, and shed in the feces; there was no evidence of bacterial replication within epithelial cells ... | 1989 | 2506781 |
| dot-enzyme linked immunosorbent assay for the detection of antibodies in bovine brucellosis. | a dot-enzyme linked immunosorbent assay (dot-elisa) for the detection of antibodies in bovine brucellosis using soluble antigens extracted from brucella abortus s-99 is described in which antigen was deposited on a nitrocellulose sheet (0.5 x 0.5 cm) bound to a plastic strip and the unsaturated sites blocked by a solution of spray-dried milk powder. sera were tested at dilutions of 1:800 and 1:1600, using rabbit anti-bovine-immunoglobulin or protein-a coupled to peroxidase as conjugates and diam ... | 1989 | 2495563 |
| brucella abortus regulates bovine macrophage-t-cell interaction by major histocompatibility complex class ii and interleukin-1 expression. | t-cell activation is dependent on nominal antigen associated with major histocompatibility complex (mhc) class ii molecules and interleukin-1 (il-1), both provided by antigen-presenting cells. we have studied the effects of brucella abortus and recombinant bovine gamma interferon (ifn-gamma) on bovine macrophage expression of mhc class ii and il-1 molecules and subsequent t-cell proliferation in response to b. abortus. when peripheral blood mononuclear cells were cocultured with b. abortus and i ... | 1989 | 2494112 |
| an elisa using an sds extract of brucella abortus strain 99 as antigen to detect b. abortus antibodies in cattle sera. | an elisa was developed using an sds extract of brucella abortus as antigen to detect antibodies in cattle sera. the antigen was stable at 4 degrees c for at least 4 years and although it gave optimal results at a 1/4,000 dilution it could detect reactors at a 1/32,000 dilution. based on comparative cf tests on 430 sera from negative herds and 187 sera from positive herds a reading of 0,07 or less using a 492 nm filter could be considered a negative reaction. the elisa could be a useful test to s ... | 1988 | 3127768 |
| histopathologic findings in brucella abortus-infected, pregnant goats. | twenty-eight pregnant goats in midgestation were exposed to a bovine pathogenic strain of brucella abortus to determine the histologic changes associated with infection. does were necropsied 0 to 7 days or 4 to 6 weeks after delivery of aborted, stillborn, or live, full-term fetuses. aborted and stillborn fetuses were necropsied within 16 hours of delivery. selected, paired tissue specimens were collected for histologic and bacteriologic examination. an avidin-biotin-peroxidase complex immunosta ... | 1988 | 3126686 |
| opsonin-dependent stimulation of bovine neutrophil oxidative metabolism by brucella abortus. | nonopsonized brucella abortus and bacteria treated with fresh antiserum, heat-inactivated antiserum, or normal bovine serum were evaluated for their ability to stimulate production of superoxide anion and myeloperoxidase-mediated iodination by neutrophils from cattle. brucella abortus opsonized with fresh antiserum or heat-inactivated antiserum stimulated production of approximately 3 nmol of o2-/10(6) neutrophils/30 min. similarly treated bacteria also stimulated the binding of approximately 4. ... | 1988 | 2831760 |
| bovine ileal dome lymphoepithelial cells: endocytosis and transport of brucella abortus strain 19. | ligated ileal loops of calves were inoculated with brucella abortus and examined at 2, 4, 6, 10, and 24 hours post-inoculation. b. abortus was identified by light and electron microscopy using immunoperoxidase and antibody-coated colloidal gold techniques. b. abortus was detected in vesicles, phagolysosomes, and large vacuoles of lymphoepithelial cells. numbers of intracellular bacteria decreased with time after inoculation. b. abortus was also seen between and below lymphoepithelial cells and f ... | 1988 | 3125659 |
| brucella abortus 1119-3 o-chain polysaccharide to differentiate sera from b. abortus s-19-vaccinated and field-strain-infected cattle by agar gel immunodiffusion. | purified brucella abortus 1119-3 and brucella melitensis 16m lipopolysaccharide o-chain polysaccharides were not precipitated in agar gel immunodiffusion by any of 24 sera from vaccinated cattle but were precipitated by 18 of 24 sera from infected cattle. this difference can be used to differentiate sera of cattle vaccinated with b. abortus s-19 from sera of some field-strain-infected cattle. | 1988 | 3133389 |
| antibody isotype response in adult cattle vaccinated with brucella abortus s19. | in a chronological study of sera collected from eight adult cattle vaccinated with 3 x 10(-10) cfu of brucella abortus s19, antibody of each of the four major isotypes was measured by indirect enzyme immunoassay (elisa) and by direct and modified complement fixation tests (cft). six of the cattle gave antibody responses to the vaccine strain that commenced between days 5 and 8 for all the isotypes in the elisa, peaked by 1 to 4 months and then declined to low levels by 10 months. direct cft and ... | 1988 | 3150885 |
| [100 years of bacteriology: the history of the discovery of brucellosis. 2: discovery of the etiology of infectious abortion in cattle by the danish veterinary pathologist bernhard bang and proof of the relation of "micrococcus melitensis" and and "bacillus abortus" by the american microbiologist alice evans]. | 1988 | 3136596 | |
| a review of enzyme immunoassay for detection of antibody to brucella abortus in cattle. | enzyme immunoassay has gained wide acceptance for serological diagnosis of bovine brucellosis because of its ability to detect antibody of all isotypes unlike the conventional tests. the indirect enzyme immunoassay, however, presents several parameters that require careful analysis. these parameters include the choice of antigen and antiglobulin-enzyme conjugate reagents for use in the assay, dealing with the large amount of data the semi-automatic or automatic assay can generate and the inter- ... | 1988 | 3137720 |
| characterization of an atypical biotype of brucella abortus. | brucella abortus strains were isolated from bovine tissue and milk samples from seven ontario herds. the isolates were characterized by colonial morphology, requirement of co2 for growth, lysis by tbilisi phage, biochemical tests and agglutination in monospecific sera. they resembled b. abortus biotype 2 (on the basis of sensitivity to thionin and basic fuchsin) and biotype 4 (on the basis of agglutination with anti-brucella "m" but not anti-brucella "a" absorbed sera). sodium dodecyl sulphate-p ... | 1988 | 3139274 |
| structural and immunochemical aspects of brucella abortus endotoxins. | smooth lipopolysaccharide (slps) of brucella abortus, which is the most immunodominant component among the antigens of b. abortus isolated, has been used for diagnosis for decades. high yields of slps can be prepared by a modification of the procedures of moreno et al. (j. bacteriol. 138:361-369, 1979). washed b. abortus cells can be disrupted by 21 freeze-quick thaw cycles and ultrasonication to separate non-membrane-bound material; then phenol extraction is performed 3 times and the phenol fra ... | 1988 | 3140612 |
| distribution of brucella abortus organisms in calves after conjunctival exposure. | thirty calves (3 to 4 months old) were exposed conjunctivally to a pathogenic strain of brucella abortus. calves were euthanatized and necropsied at postexposure hours 2 and 4, and at postexposure days (ped) 1, 4, 7, 14, 21, 42, and 49. selected ocular, pharyngeal, and lymphoid tissues were cultured bacteriologically for brucellae to determine organism distribution. brucella abortus organisms initially localized in the third eyelids, bulbar conjunctivae, and parotid lymph nodes and were detected ... | 1988 | 3149162 |
| further evidence that bovine igm does not fix guinea pig complement. | in a study of sera from cattle vaccinated with 3 x 10(10) cfu of brucella abortus strain 19, it was found that igg1 antibody measured by an indirect elisa was the only isotype to correlate with standard complement fixing antibody titers using heated serum samples and guinea pig serum as a source of complement. a supplement of normal unheated bovine serum resulted in igm fixing guinea pig complement, giving data similar to those obtained with unheated serum in the complement fixation test. | 1988 | 3150884 |
| failure to isolate brucella abortus from embryos or ova from culture-positive superovulated cows. | nine, brucella abortus culture positive 2-yr-old cows were used to test the hypothesis that embryos and ova collected from such cows are not infected. superovulation was induced at varying times postpartum or postabortion with intramuscular injections of follicle stimulating hormone (fsh). the cows were artificially inseminated with b. abortus-negative semen. superovulations and nonsurgical embryo collections nonsurgical embryo collections were attempted twice for each cow. jugular blood, udder ... | 1988 | 16726359 |
| assessment of bovine mammary gland macrophage oxidative burst activity in a chemiluminescence assay. | a major bactericidal mechanism of neutrophils and macrophages is the generation of toxic oxygen-free radicals upon phagocytosis of microbes. studies were conducted to assess the oxidative metabolism of bovine mammary gland macrophages. bovine mammary gland macrophages were challenge exposed with a variety of phagocytic stimuli in an in vitro, luminol-assisted chemiluminescence assay. a measurable oxidative burst was observed when macrophages were challenge exposed with heat-aggregated bovine imm ... | 1987 | 3826830 |
| bovine igm: does it fix guinea pig complement in the absence of bovine complement components? | purified bovine isotypes igm, igg1, igg2, and iga (secretory), affinity purified with brucella abortus, were tested in a complement fixation test (cft) for their ability to activate guinea pig complement directly or in the presence of 'normal' bovine serum. only igg1 fixed guinea pig complement in the direct test and approximately 250 ng of antibody was required to activate 50% of 3 ch50 units with a standard amount of antigen. addition of 'normal' bovine serum as an additional source of complem ... | 1987 | 3111076 |
| preparation by ultrafiltration and control by high-performance liquid chromatography of the native hapten of brucella abortus for use in radial immunodiffusion diagnostic test. | an effective method was developed for the preparation of brucella abortus native hapten by use of an ultrafiltration system. the membranes pm30 and ym5 were used to separate the lipopolysaccharide and the native hapten. the yield of the native hapten was higher than that obtained by a more complex procedure reported previously. this method is economical for the large-scale preparation of b. abortus native hapten. the effects of ultrafiltration were evaluated by a quick and sensitive high-perform ... | 1987 | 3117841 |
| effect of bovine non-agglutinating antibodies on the blood clearance of 131i-labelled brucella abortus strain 45/20. | non-agglutinating anti-brucella abortus s45/20 antibodies were isolated and purified from sera of immunized cattle by means of immunoadsorption and ion-exchange chromatography (deae-sephadex a-50). they corresponded to the igg1 isotype as shown by immunoelectrophoresis using monospecific anti-igg1 and anti-bovine gamma globulin sera. these antibodies failed to agglutinate the antigen. they were detected by the anti-bovine gamma globulin test, showing higher titres than those of agglutinating ant ... | 1987 | 3125666 |
| some aspects of experimental bovine brucellosis. | in the course of eight successive brucella abortus experiments, 394 cows were followed by clinical, serological and bacteriological examinations. cultures were made from vaginal swabs and colostrum at delivery and from 10 pools of lymph nodes or organs sampled at slaughter, about 6 weeks after calving. from these cows, 346 had been experimentally challenged with 1.5 x 10(7) b abortus strain 544 via the conjunctiva, 3 congenitally infected and 45 bought from known naturally infected herds. males ... | 1987 | 3132076 |
| antibodies in calves on feed supplemented with chlortetracycline after vaccination with brucella abortus strain 19. | twenty dairy heifers each consumed 350 mg of chlortetracycline/day in their feed. four tests were performed on serum specimens from these and 20 control calves after vaccination with brucella abortus strain 19. the numbers of positive test results on the card test and mean titers on the tube and rivanol agglutination and complement-fixation tests were compared in the 2 groups. using the rivanol and complement-fixation tests, there were differences in the mean titers at weeks 5 and 6 after vaccin ... | 1987 | 3106273 |
| the identity, distribution and epizootiological significance of brucella isolates in australia, 1981 to 1985. | results listing the identification of brucella isolates received by the national brucellosis reference centre, national biological standards laboratory, canberra from 1981 to 1985 are presented. the distribution of brucella species and biotypes is shown on a host and state basis. cultures isolated in australia were identified as brucella abortus biotypes 1, 2 and 4, and strain 19; b. suis biotype 1, and b. ovis. b. melitensis biotype 3 was recovered from man infected in the mediterranean area. b ... | 1987 | 3111457 |
| immunochemical and partial chemical characterization of fractions of membrane-bound smooth lipopolysaccharide-protein complex from brucella abortus. | smooth lipopolysaccharide (slps) of brucella abortus was prepared and fractionated by a modification of the procedures of moreno et al. (j. bac. 138:361-369, 1979). washed b. abortus cells were disrupted by 21 freeze-quick thaw cycles with ultrasonication to separate the non-membrane-bound material. ultrasonicated bacteria were used for preparation of membrane-bound slps (approximately f5, the main crude slps fraction described by moreno et al.). phenol extraction was repeated 3 times and then w ... | 1987 | 3114618 |
| ultrastructural morphometric analysis of brucella abortus-infected trophoblasts in experimental placentitis. bacterial replication occurs in rough endoplasmic reticulum. | trophoblasts in normal and brucella abortus-infected caprine placentas were examined by ultrastructural morphometric analysis to establish structural relationships of b abortus with cytoplasmic organelles; brucellae were identified with colloidal gold-labeled anti-b abortus bovine igg. cytotrophoblasts had large numbers of b abortus in cisternae of rough endoplasmic reticulum; binucleate trophoblasts did not contain bacteria. in infected trophoblasts there was a significant hypertrophy of b abor ... | 1986 | 3090887 |
| comparison of the results of card, rivanol, complement-fixation, and milk ring tests with the isolation rate of brucella abortus from cattle. | specimens from 4,553 cows were examined by card, rivanol, and complement-fixation (cf) tests and bacteriologic culture. a ring test was performed on milk from 1,003 of these cows. the isolation rate of brucella abortus correlated directly with antibody titers, and the field strain predominated in adult-vaccinated cows when the rivanol test titer was greater than + 100 and the cf test titer was greater than 4 + 40. the cf test had the best balance of sensitivity and specificity in adult-vaccinate ... | 1986 | 3090911 |
| production of auto-anti-idiotypic antibody during the normal immune response. xii. enhanced auto-anti-idiotypic antibody production as a mechanism for apparent b-cell tolerance in rabbits after feeding antigen. | rabbits fed trinitrophenylated bovine serum albumin (tnp-bsa) generated fewer anti-tnp plaque-forming cells but greater numbers of hapten (tnp)-augmentable igm and igg pfc following immunization with tnp-ficoll or tnp-brucella abortus than did animals not previously fed antigen. spleen and mesenteric and bronchial lymph nodes were similarly affected. in addition more auto-anti-idiotype (id) antibody (anti-anti-tnp) was eluted by hapten from spleen cells of antigen-fed rabbits than from spleen ce ... | 1986 | 3091266 |
| identification of brucella abortus in formalin-fixed, paraffin-embedded tissues of cows, goats, and mice with an avidin-biotin-peroxidase complex immunoenzymatic staining technique. | an avidin-biotin-peroxidase complex immunoenzymatic staining technique was evaluated for light microscopic detection of brucella organisms in formalin-fixed, paraffin-embedded tissues. tissues from cows, goats, and mice inoculated with b abortus strain 2308 were examined, using rabbit antiserum to brucella cell surface protein as primary antibody. stained organisms were identified histologically in tissue sections containing b abortus, as detected by bacteriologic examination of duplicate nonpro ... | 1986 | 3096172 |
| the relationship between the isolation of brucella abortus and serological status of infected, non-vaccinated cattle. | seventy two non-vaccinated cattle with various complement fixation (cf), rose bengal (rb) and enzyme-linked immunosorbent assay (elisa) results at slaughter were examined bacteriologically and serologically. brucella abortus was recovered from 49 (68.1%) of the cattle and the use of a biphasic culture medium was entirely responsible for the detection of 6 (12.2%) of the culture positive cattle. the supramammary and retropharyngeal lymph nodes were the most rewarding tissues to culture. a compari ... | 1986 | 3094489 |
| distinct fixation of defined amounts of complement by the brucella abortus antigen and bovine antibodies under different incubation conditions in the cft. | different times and temperatures of the 1st phase incubation of the complement fixation test (cft) were used. it was found that a similar decrease in complement (c') activity occurred after 2 h at 37 degrees c, after 8 h at 20 degrees c and after 18 h at 4 degrees c. simultaneously, in the same periods a double increase in standard serum titre at 4 and 20 degrees c was noted in comparison with titre at 37 degrees c. this increase of sensitivity of the cft at 4 and 20 degrees c appeared to be a s ... | 1986 | 3100131 |
| [standardization of the complement fixation test (cft) in brucellosis. i. the polish standard of anti-brucella abortus serum]. | the purpose of this paper was to prepare an indigenous standard of anti-brucella abortus serum for the complement fixation test (cft) as a homologue of the secon international standard of anti-brucella abortus serum (isabs-ii), which contains 1000 international units of complement-fixing antibodies in 1 cm3. the indigenous standard of anti-brucella abortus serum (isabas) was prepared in 1975. the material used was the serum of a cow infected with br.abortus, biotype 1, under natural conditions. ... | 1986 | 3103110 |
| localization of antigen-specific lymphocytes following lymph node challenge. | the effect of subcutaneous injections of brucella abortus strain 19 antigen on the specific localization of autologous lymphocytes in the regional nodes of calves was analysed by fluorescent labelling and flow cytometry. both in vitro and in vivo fitc labelling of lymphocytes indicated the preferential migration of lymphocytes from a previously challenged lymph node to a recently challenged lymph node. however, lymphocytes from a lymph node challenged with b. abortus failed to localize preferent ... | 1986 | 2426183 |
| comparison of an enzyme-linked immunosorbent assay using monoclonal antibodies and a complement fixation test for cattle vaccinated and infected with brucella abortus. | a competitive enzyme-linked immunosorbent assay (elisa) using a monoclonal antibody conjugated to horseradish peroxidase ma(a) and a complement fixation test (cft) were applied to sera collected over a two-year period from 60 cattle challenged with brucella abortus strain 544. forty-eight of the cattle were previously vaccinated with b. abortus strain 19 (s19) or b. abortus strain 45/20 (45/20). after challenge 33 of the cattle remained uninfected and nine of the 27 infected cattle showed aberra ... | 1986 | 3088822 |
| preliminary evidence for a diagnostic immunoglobulin g1 antibody response among culture-positive cows vaccinated with brucella abortus strain 19 and challenge exposed with strain 2308. | the sera of cows inoculated with brucella abortus have a characteristically high titer of immunoglobulin (ig) g1 antibodies to a soluble brucella antigen compared with sera of noninoculated vaccinated cattle. concentrations of antigen-specific igg1 were greater than 10-fold higher than those for igg2, even though total igg2 concentrations were higher than total igg1 concentrations. increases in igg1 antibodies to brucella abortus soluble antigen were detected shortly after vaccination in those c ... | 1986 | 3089082 |
| pathogenesis of placentitis in the goat inoculated with brucella abortus. i. gross and histologic lesions. | pregnant goats were given brucella abortus intravenously or in uterine arteries, and tissues from the uterus and placentae were examined at various post-inoculation intervals to study mechanisms of placental infection. placentitis was present by 5 days post-inoculation and abortions occurred within 11 days. b. abortus was identified in placentae by light microscopy and immunoperoxidase techniques. b. abortus was first seen in erythrophagocytic trophoblasts of the placentome. subsequently, high n ... | 1986 | 3088809 |
| resistance of preimplantation bovine embryos to infection with brucella abortus. | preimplantation bovine embryos were exposed in vitro to brucella abortus to determine if the bacteria would adhere to zona pellucida (zp)-intact embryos or adhere to or infect zp-free embryos. brucella abortus was not isolated from zp-intact or zp-free groups of embryos after 10 sequential antibiotic-free washings. brucella abortus was isolated from all groups containing zp-defective embryos after the exposure period and washing. detrimental effects on healthy in vitro development of embryos wer ... | 1986 | 3767099 |
| detection of antigenic fractions from brucella abortus s45/20 which bind to non-agglutinating antibodies using electroblotting and enzyme-linked antibody probes. | outer membrane antigens which bind to non-agglutinating antibodies (naab) elicited by smooth (s19) and rough (s45/20) brucella abortus strains, were extracted from s45/20 by stirring in cold 2.5% nacl and then analyzed by sds-page, electroblotting and enzyme-linked antibody test. eight bands were observed in the gel stained with coomassie blue. seven antigenic fractions were transferred to nitrocellulose by blotting. a 27-kd band was recognized by bovine anti-s45/20 non-agglutinating serum and n ... | 1986 | 3097916 |