Publications

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[preliminary study of retriculate varieties of biomphalaria glabrata (say 1818) in comparison with pigmented and albino strains]. 1978653218
[selection in strains of biomphalaria tenagophila and biomphalaria glabrata for improving susceptibility to schistosoma mansoni]. 1978675140
relationship between a species of malaysian nematode daubaylia and the schistosome intermediate host biomphalaria glabrata. 1978749232
[molluscicidal properties of the euphorbia cotinifolia l (author's transl)].the hexanic extract from the leaves of euphorbia cotinifolia l. has been experimentally used as molluscicide against biomphalaria glabrata. the extract was fractionated and the most actives fractions were those of numbers 6 and 7. the lethal concentrations (lc50 and lc90 obtained using fraction 6 against adult snails were 1,2 and 2,4 ppm; for the eggs they were 25,0 and 48,0 ppm. with fraction 7 the lethal concentrations were 1,4 and 3,4 ppm for adult snails; for newly hatched snails they were 4 ...1978751112
[susceptibility of a strain of biomphalaria glabrata from venezuela to infection with 2 different strains of schistosoma mansoni]. 1978751168
a simple quantitative technique for testing behavioral responses of schistosoma mansoni miracidia to chemicals.we describe a new technique for testing responses of schistosoma mansoni miracidia to chemicals. miracidia in spring water were placed in a chamber shaped like the greek letter phi. small volumes of test chemicals were inoculated into one side of the chamber. after 30 sec a dam was inserted to bisect the chamber and the percentage of miracidia on the inoculated side was calculated. reproducible quantitative results were obtained using the known miracidial stimulants, snail-conditioned water (bio ...197825318
biomphalaria glabrata: lysozyme activities in the hemolymph, digestive gland, and headfoot of the intermediate host of schistosoma mansoni. 1978103747
susceptibility of bahian population of biomphalaria glabrata to an allopatric strain of schistosoma mansoni.seventeen populations of snails (biomphalaria glabrata) from the state of bahia, brazil were studied with respect to their susceptibility to infection with an allopatric strain of schistosoma mansoni. two of the populations were from the city of salvador, 3 from other sites in bahia, and 12 from habitats in the municipio castro alves. our data show that the bahian snail populations are extremely variable in their susceptibility to an allopatric strain of s. mansoni and that the phenomenon is not ...1978686244
schistosoma mansoni: identification of chemicals that attract or trap its snail vector, biomphalaria glabrata.a new bioassay for chemical attractants of aquatic snails demonstrated that biomphalaria glabrata could be attracted to or trapped in the vicinity of homogenates of lettuce. fractionation of homogenates revealed the amino acids glutamate and proline and the primary attractants. attraction was specific for the l form of glutamate. proline appeared to stimulate reproductive activity. glutathione, gamma-aminobutyric acid, and a number of other compounds had no effect. extracts of lyophilized snail ...1978684418
hemocytes of biomphalaria glabrata: factors affecting variability. 1978731071
schistosoma mansoni: lysozyme activity in biomphalaria glabrata during infection with two strains. 1978729698
[susceptibility of biomphalaria glabrata, albino variant, from belo horizonte, mg, to infestation by schistosoma mansoni, a parasite under natural conditions, of wild rodents of the vale do rio paraiba do sul, sp (brazil)]. 1978715359
an automatic method for recording the emergence of cercariae of schistosoma mansoni from the snail biomphalaria glabrata.in order to study the pattern of emergence of trematode larvae from the snail hosts (s. mansoni from b. glabrata) an automatic apparatus was developed. this apparatus allows the snails, each suspended by a thread, to be transported at intervals of at least half an hour into different beakers into which the cercariae are shed. this "eclosion clock" is essentially a carriage driven on perlon wheels along steel bars.1978695822
[fertility of helisoma (seminolina) duryi (wetherby 1879) and biomphalaria glabrata (say 1818), cultured in laboratory (pulmonata, planorbidae)]. 1978549153
[freshwater snails and vectors of schistosomiasis mansoni in martinique (french west indies)].an inventory of the freshwater molluscs in martinique was worked out during 1972 to 1978. each of the 19 species is shown with its main characteristics. the distribution of biomphalaria glabrata and biompharia straminea was studied. the epidemiological importance of these two snails is discussed.1979575471
effect of schistosoma mansoni infection on fecundity and perivitelline fluid composition in biomphalaria glabrata.egg production in the snail, biomphalaria glabrata, infected with schistosoma mansoni declined on day 23 postinfection, and was significantly lower than uninfected control snails by day 28 and thereafter. protein and galactogen content of eggs produced by infected snails did not change during the period of reduced fecundity. this suggests that decreased hemolymph nutrient levels (rather than depleted albumen gland reserves) are responsible for inhibition of snail egg production. growth rates of ...1979575549
competitive interaction between two snail hosts of schistosoma mansoni: laboratory studies on biomphalaria glabrata and b. straminea. 1979549209
host reactions in biomphalaria glabrata to schistosoma mansoni miracidia, involving variations in parasite strains, numbers and sequence of exposures. 1979541169
capacity of irradiated echinostome sporocysts to protect schistosoma mansoni in resistant biomphalaria glabrata. 1979541168
[experimental infection of biomphalaria glabrata with schistosoma mansoni in the presence of helisoma duryi]. 1979262314
[pomacea haustrum (reeve, 1856) as a decoy to infection of biomphalaria glabrata (say, 1818) with schistosoma mansoni sambon, 1907]. 1979262318
[susceptibility of biomphalaria tenagophila and biomphalaria glabrata to 2 strains of schistosoma mansoni]. 1979482771
schistosome sporocyst-killing amoebae isolated from biomphalaria glabrata. 1979501126
radioisotope labeling of schistosoma mansoni miracidia for in vivo studies in biomphalaria glabrata. 1979501130
[evaluation of a trial to control biomphalaria glabrata in guadeloupe by using a sterilizing trematode (author's transl)].a trial of biological control of biomphalaria glabrata was carried out in guadeloupe by using ribeiroia marini guadeloupensis nassi, 1978, an autochtonal trematode which sterilizes the snail vector of schistosoma mansoni. the trial took place in a pond in which the population of b. glabrata presents an annual demographic cycle related to the alternation of dry and rainy seasons. about 8 millions eggs of r. marini were introduced in 15 months into the biotope. despite the low yield of these intro ...1979539719
bge snail cell-line antigens: ineffectiveness as antischistosomal vaccine in mice.mice and rabbits were immunized with antigens derived from bge cells, biomphalaria glabrata hemolymph, or schistosoma mansoni. antisera from mice given molluscan antigens did not form immunoprecipitates with soluble antigen from adult worms, but their binding to surfaces of sporocysts, cercariae, and schistosomules suggests the presence of cross-reacting determinants. in vitro, cell-mediated immune responses to bge antigens were not demonstrable in infected nor in immunized mice. mice immunized ...1979120433
alterations of the free amino acid content in the hemolymph of biomphalaria glabrata (pulmonata) in starvation and after infection with schistosoma mansoni (trematoda).1. analysing the free amino acid content of the hemolymph of normally fed, starved and schistosome-infected biomphalaria glabrata we identified 22 amino acids and amines, respectively. 2. in comparison with normally fed snails the free amino acid content of starved and infected snails was significantly reduced. 3. however, only slight differences between the ornithine and citrulline content occurred in the three test groups.1979318425
specificity of natural resistance to trematode infections in biomphalaria glabrata. 1979541166
[intestinal schistosomiasis in the freshwater mangrove of guadalupe (french antilles). ecology of the vector, biomphalaria glabrata and its parasite schistosoma mansoni (author's transl)].in the freshwater mangrove of dubelloy-devarieux (guadalupe), the dynamics of populations of b. glabrata and the transmission of s. mansoni, mostly depends on the alternation of dry and rainy season. the flooding of the mangrove (in september), permits a yearly renewal of the malacological populations. the sizes analysis of the snails shows that the life cycle of b. glabrata include two rainy seasons separated by one dry season. the prevalence of b. glabrata (0,25%) and cercariae densities (0,8 ...1979485041
comparison of four species of snails as potential decoys to intercept schistosome miracidia.preliminary studies have shown that various species of aquatic snails may be used as decoys or "sponges" to intercept schistosome miracidia, thereby preventing the miracidia from reaching the snails which normally serve as their intermediate host. in this study, four species of snails were evaluated as candidate decoys for field trials: marisa cornuarietis, pomacea australis, helisoma caribaeum, and tarebia granifera. in the laboratory all four species caused considerable reductions in the propo ...1979434319
behavioural responses to amino acids and related compounds, including propionic acid, by adult biomphalaria glabrata (say), a snail host of schistosoma mansoni. 19806104559
[liberation of schistosoma mansoni, sambon, 1907 cercariae by biomphalaria glabrata (say, 1818) maintained in a medium with magnesium thermophosphate]. 19806294756
the destruction of schistosoma mansoni mother sporocysts in vitro by amoebae isolated from biomphalaria glabrata: an ultrastructural study. 19807365267
differential leukocytic responses of biomphalaria glabrata to infection with schistosoma mansoni. 19807365270
infectivity of a microsporidium of mosquitoes (nosema algerae) to larval stages of schistosoma mansoni in biomphalaria glabrata. 19806778833
inducement of miracidia-immobilizing substance in the hemolymph of biomphalaria glabrata. 19807409973
metabolic interrelationship of parasitic trematodes and molluscs, especially schistosoma mansoni in biomphalaria glabrata. 19807456639
abnormal development of the digestive gland in biomphalaria glabrata. 19807440998
therapeutic efficacy of oral oxamniquine in the toxemic form of schistosomiasis mansoni: treatment of eleven individuals from two families, and experimental study.two families, comprising 11 individuals in the toxemic form of schistosomiasis mansoni, infected in belo horizonte, brazil were treated. parasitological cure was obtained in 5 (45%) of the patients after a single oral dose of oxamniquine (mansil), 20 mg/kg body weight. no significant side effects were observed. to evaluate the possibility of resistance to the drug, cercariae collected from biomphalaria glabrata infected with micracidia from eggs obtained from three of the individuals not cured w ...19807352627
biomphalaria glabrata amoebocytes: effect of schistosoma mansoni infection on in vitro phagocytosis. 19807373069
macrophagelike hemocytes of resistant biomphalaria glabrata are cytotoxic for sporocysts of schistosoma mansoni in vitro.when schistosoma mansoni miracidia penetrate resistant individuals of the intermediate host snail biomphalaria glabrata, the sporocyst is encapsulated by hemocytes (macrophagelike cells of the snail circulation) and killed. in our in vitro model the same fate requires only sporocysts and snail hemolymph. however, when cultured in plasma alone (cell-free hemolymph), sporocysts remain viable for more than 3 days, regardless of whether the plasma is from susceptible or resistant snails. when hemoly ...19807391885
schistosoma mansoni: cytotoxicity of hemocytes from susceptible snail hosts for sporocysts in plasma from resistant biomphalaria glabrata. 19807428914
tissue reactions induced by schistosoma mansoni in biomphalaria glabrata.in biomphalaria glabrata with a strong natural resistance, schistosoma mansoni sporocysts are rapidly encapsulated by granulocytes and killed, mainly by the strong phagocytic activity of the cells. irradiated echinostoma paraensei sporocysts seem able to suppress the function of the granulocytes. tissue reactions in snails with self-cure demonstrate: involvement of two types of cells, granulocytes and hyalinocyte-like cells; formation of amoeba-fibrous capsules; limited tendency of granulocytes ...19807436601
schistosoma mansoni control in cul de sac valley, saint lucia. i. a two-year focal surveillance-mollusciciding programme for the control of biomphalaria glabrata.an area-wide mollusciciding campaign in cul de sac valley, st. lucia reduced incidence of schistosoma mansoni from 22% to 4.3% between 1970 and 1975. following this, a two-year focal surveillance-mollusciciding programme was introduced. sites of potential transmission of s. mansoni were identified and routinely searched for biomphalaria glabrata. if found, the site was treated with clonitralide 25% emulsifiable concentrate. two chemotherapy campaigns supplemented the snail control programme. as ...19807445045
[biological competition between biomphalaria glabrata (say, 1818) and biomphalaria tenagophila (d'orbigny, 1835), in natural breeding areas in the municipality of ourinhos, s.p. (brazil)]. 19807433874
the boqueron project after 5 years: a prospective community-based study of infection with schistosoma mansoni in puerto rico.the boqueron schistosomiasis project is a prospective community-based study of schistosoma mansoni infection after the interruption of transmission by nonchemotherapeutic control measures. the study methods and the parasitologic results of the first five annual stool surveys are described in this report. in the first year, 1972, among 904 inhabitants (88% of the total population) the prevalence of infection was 40%, and the geometric mean intensity of infection among positives was 16.1 eggs per ...19807446813
[cellular immunity in mollusk hosts of schistosomes. primary results regarding biomphalaria glabrata].cell culture are obtained from the saccular part of the kidney of biomphalaria glabrata in a medium derived from schneider drosophilia medium. two types of cells migrate from the explant: numerous amoebocytes and some fibroblasts. migration of amoebocytes appears to be regular. these amoebocytes are able to encapsulate and destroy particules and dead miracidiums. amoebocytes from incompatible snails rapidly surround miracidiums and early larvae (primary sporocysts) whereas compatible miracidium ...19807460123
[life cycle of clinostomum golvani n. sp. (trematoda : clinostomidae) a larval parasite of biomphalaria glabrata, the snail vector of schistosoma mansoni in guadeloupe (author's transl)].the life cycle of a new species of clinostomidae, clinostomum golvani, described in guadeloupe (french west indies). the first intermediate host is the snail vector of schistosoma mansoni in this island, biomphalaria glabrata, which can be sterilized by this parasite. poecilia reticulata (guppy) serves as the second intermediate host. adult worms were obtained under experimental conditions from butorides virescens (the definitive host in nature), nycticorax nycticorax and ardea purpurea. the adu ...19807224533
[susceptibility to schistosoma mansoni infection of biomphalaria glabrata and biomphalaria tenagophila in the federal district, brazil]. 19817345560
schistosoma mansoni: effect on growth, fertility, and development of distal male organs in biomphalaria glabrata exposed to miracidia at different ages.growth, egg laying rate, and mortality of biomphalaria glabrata (portorican strain), maintained in groups of two, after exposure at different ages (1, 2, 4, 6, 8 weeks) to three miracidia each of schistosoma mansoni (liberian strain) were determined. the findings in infected snails were compared with those in exposed but uninfected (negative) and in unexposed snails. younger snails were infected at higher rates, and these showed a significantly increased mortality. young infected snails grew muc ...19817324545
reduction in transmission of schistosoma mansoni by a four-year focal mollusciciding programme against biomphalaria glabrata in saint lucia.the effect of transmission of schistosoma mansoni of a focal snail control programme was investigated over four years amongst approximately 1250 people living in five communities in the steep-sided soufriere river valley, st. lucia, west indies. bayer 6076 was applied from constant flow drip cans to 12 stream sections at a target dose of 8 mg/litre clonitralide every four weeks. only proven and potential transmission sites were treated; marsh habitats, where biomphalaria glabrata were widespread ...19817330940
[evolution of spopocysts of schistosoma mansoni sambon 1907 in biomphalaria glabrata (say 1818) and biomphalaria tenagophila (d'orbigny 1835)]. 19817336119
resistance to schistosome infection in biomphalaria glabrata induced by gamma radiation. 19817196934
experimental epidemiology of schistosomiasis. ii. prepatency of schistosoma mansoni in biomphalaria glabrata at diurnally fluctuating temperatures.the basic hyperbolic relationship established between constant water temperatures and the length of the prepatent period in the snail was tested as diurnally fluctuating temperatures, stimulating natural conditions. diurnal temperature variations are generally summed up by s. mansoni according to the time-temperature product and are independent of the shape of the temperature curve. using a simple hyperbolic formula, the time of prepatency can be calculated for temperatures ranging from 16 degre ...19817198850
dynamics of larval populations of schistosoma mansoni in biomphalaria glabrata. i rhythmic production of cercariae in monomiracidial infections. 19817271357
[contribution to the study of nervous system in larval and adult forms of schistosoma mansoni sambon, 1907 (trematoda digenea). ii. role of serotonine and dopamine (author's transl)].this study provides details concerning the localization and role of 5-ht and da in schistosoma: 1) 3h-5ht is incorporated selectively by the nerve fibers containing dense granules measuring 820 +/- 20 a (type 2 granules), combined at times with other structures; the presence of "cold" dopamine in the environment does not alter the incorporation; 2) 3h-da is also incorporated in vitro by the nerve fibers and occasionally by the muscle fibers of the adult schistosoma; 3) neither the intraovular mi ...19817316398
dynamics of larval populations of schistosoma mansoni in biomphalaria glabrata ii. chronobiology of the intramolluscal larval development during the shedding period. 19817316580
selective interference with granulocyte function induced by echinostoma paraensei (trematoda) larvae in biomphalaria glabrata (mollusca).various trematode larvae can interfere with the host snail's resistance to the same or unrelated trematode species, chiefly, it appears by interference with the function of the host's granulocytes. in biomphalaria glabrata infected with the trematodes, echinostoma paraensei, granulocytes lose their ability to encapsulate the larvae of trematodes to which the hosts were previously resistant. however, the granulocytes in these snails retain their ability to encapsulate injected latex spheres, or l ...19817328451
schistosoma mansoni, nih-sm-pr-2 strain, in susceptible and nonsusceptible stocks of biomphalaria glabrata: comparative histology.of 10 inbred stocks of biomphalaria glabrata examined, eight were susceptible to the nih-sm-pr-1 strain (pr-1) of schistosoma mansoni, whereas only three were susceptible to the nih-sm-pr-2 strain (pr-2). by employing a histological ranking system based on parasite development and host tissue response, we have observed a range of nonsusceptibility among stocks nonsusceptible to pr-2. in three nonsusceptible stocks, sporocysts were rapidly surrounded by host amebocytes and were destroyed within a ...19817299581
an isoenzyme marker possibly associated with the susceptibility of biomphalaria glabrata populations to schistosoma mansoni.nine laboratory populations and one field population of the snail host biomphalaria glabrata were compared with respect to their electrophoretic patterns for acid phosphatase (acp) and with their susceptibility to schistosoma mansoni infection. a strong correlation (r = 0.98) was noted between the frequency of the isoenzymes acp2-s and acp2-f observed in the populations and the level of snail susceptibility as determined by bioassay. the isoenzyme acp2-s was associated with susceptibility, acp2- ...19816123248
[investigations on periodic phenomena in the snail biomphalaria glabrata (author's transl)].a method was developed to measure the locomotive activity of biomphalaria glabrata over long periods of time. a snail, radioactively marked with a co57-source, moves on a circular path. beneath the experimental vessel a lead disc with a slit revolves (one revolution per minute) over a counter tube. once every minute the amount of radioactivity emitted falls through the slit onto the counter tube. the position and thence the locomotive activity of the snail is determined from the intervals betwee ...19816894208
concanavalin a-induced receptor redistribution on biomphalaria glabrata hemocytes: characterization of capping and patching responses. 19817024423
[study of the mechanisms of rejection in incompatible mollusk-schistosome pairs from infestations by means of a natural route or by microsurgical transplantations of parasitic stages].the non-compatible host-parasite combination used in these studies is an albino brazilian strain of biomphalaria glabrata (bre) and a guadeloupean strain of schistosoma mansoni (gua). this work allows us to emphasize three main points: --the failure of infestation by miracidia transplantations in the non-compatible combination indicates that the origin of resistance does not lie at the level of the tegument, but further away, in the hemolymph of the host. --the results of the histological studie ...19826131592
swellengrebel lecture: survival of schistosoma mansoni and other trematode larvae in the snail biomphalaria glabrata. a discussion of the interference theory. 19827123644
amebocytic accumulations in biomphalaria glabrata: fine structure.internal pathologic conditions in four stocks of biomphalaria glabrata involve amebocytes. most snails exhibiting these conditions are also nonsusceptible to infection with schistosoma mansoni. fine structure studies reveal that periaortic and atrial amebocytic accumulations show two responses that involve heart tissue: a generalized response in which amebocytes have infiltrated the myocardium and focal areas of nodules in which heart tissue is encapsulated and destroyed. in pericardial accumula ...19827128891
ultrastructure of encapsulation of schistosoma mansoni mother sporocysts by hemocytes of juveniles of the 10-r2 strain of biomphalaria glabrata.dynamic aspects of the cellular responses of juvenile (2-3 mm shell diameter) 10-r2 strain biomphalaria glabrata to newly penetrated schistosoma mansoni (nih-sm-pr-1 strain) were studied at the ultrastructural level. as early as 3 hr postexposure (pe), host hemocytes had contacted the parasite's surface and by 7.5 hr, had phagocytosed sporocyst microvilli and small pieces of underlying tegument. most sporocysts observed at 24 hr pe lacked tegumental cytoplasm, and germinal cells and other intern ...19827077450
in vitro encounters between schistosoma mansoni primary sporocysts and hemolymph components of susceptible and resistant strains of biomphalaria glabrata.encounters between axenically transformed sporocysts of the puerto rican 1 strain of schistosoma mansoni and hemocytes and plasmaaa components of susceptible "m line" and resistant 10-r2 and 13-16-1 strains of biomphalaria glabrata were studied in vitro. after 24 hours of incubation in susceptible hemolymph components, 94.4% of 36 observed sporocysts retained normal structure, whereas 9l.6% of 48 sporocysts were destroyed after 24 hours of incubation in resistant hemolymph components. sporocysts ...19827125067
transmission of schistosoma mansoni from man to snail: laboratory studies on the influence of snail and miracidial densities on transmission success.the relationship between infection success, the density of schistosoma mansoni miracidia and the density of the snail host biomphalaria glabrata was examined experimentally. within laboratory infection arenas, the distribution of miracidial infections/snail was approximately uniform and the net rate of snail infection was directly positive binomial to miracidial density. in contrast, however, the rate of infection declined exponentially as snail density increased. mathematical models are develop ...19827145477
use of praziquantel in patients with schistosomiasis mansoni previously treated with oxamniquine and/or hycanthone: resistance of schistosoma mansoni to schistosomicidal agents.fourteen patients with active schistosomiasis mansoni in spite of previous treatment with oxamniquine and/or hycanthone were treated with praziquantel, single oral dose of 45 to 50 mg/kg body-weight. all underwent clinical, laboratory and electrocardiographic examination before and after treatment. untoward effects (dizziness, drowziness, nausea and abdominal pain) were observed in ten. laboratory findings disclosed no significant alteration and the electrocardiograms showed no abnormalities. mo ...19827179419
[comparative study of the susceptibility of biomphalaria glabrata populations of belo horizonte (mg), to schistosoma mansoni infection and to molluscacides]. 19827182912
[evaluation of 10 years of research on s. mansoni schistosomiasis a in the french west indies].from 1971 until 1982, we studied the ecological factors which stead fast a perennial schistosomiasis in guadeloupe on an exhaustive mode. more recently, we start a similar investigation in martinique. in guadeloupe the single vector of schistosoma mansoni is the snail biomphalaria glabrata which is an ubiquist species. but the transmission to man is restricted to the "canal-sewers" where faeces are dropped and not in the rivers of basse-terre or the ponds of grande-terre. moreover, the infection ...19827126776
evaluation of chemotherapy in the control of schistosoma mansoni in marquis valley, saint lucia. ii. biological results.following the final chemotherapy campaign of a 4-year program, the biological assessment of transmission of schistosoma mansoni was evaluated for 5 years. no infected biomphalaria glabrata were found amongst 28,791 and 13,550 snails examined from flowing and static index sites, respectively. however, 89 infected snails of 16,602 examined (0.54%) were found from additional sites. sentinel snails examined between march 1974 and october 1977 revealed no infections and their use was discontinued. fo ...19827058972
schistosoma mansoni: quantification of skin penetration and early migration by differential external radioassay and autoradiography.eleven gamma-emitting radionuclides (49sc, 54mn, 59fe (+2 and +3), 60co, 65zn, 75se (as selenomethionine, selenocystine, selenite and selenate), 109cd, 125sb, 133ba, 137cs and 203hg) were screened as labelling agents for schistosoma mansoni cercariae by incubation of infected biomphalaria glabrata snails in radioactive solution according to the technique of christensen (1977). only [75se]methionine yielded satisfactorily labelled cercariae. multiple regression analysis of volume, number of cerca ...19826803218
effect of schistosoma mansoni on glycogen synthase and phosphorylase from biomphalaria glabrata (mollusca). 19826804614
further characterization of acquired resistance in biomphalaria glabrata.the albino strain of biomphalaria glabrata is capable of developing an acquired resistance to echinostomes that is relatively specific, i.e., strong against echinostoma lindoense, challenged by the homologous species, moderate or weak against closely related e. paraensei and e. liei, and nonexistent against paryphostomum segregatum and schistosoma mansoni. the present results show that acquired resistance induced by e. paraensei is much stronger against the heterologous e. lindoense than against ...19827119983
effects of starvation and infection with schistosoma mansoni on the release rate of free amino acids (faa) by biomphalaria glabrata.1. the elimination of dissolved free amino acids (faa) by individual snails during a period of 2 hr was measured. the faa concentration in the hemolymph and in the homogenate also was determined. 2. for uninfected, normally fed snails a daily faa production of 0.7 +/- 0.25 mumol glycine equivalents/g live wt was found. with snails starving for 7 days the daily faa release rate fell to a mean value of 0.44 +/- 0.19 mumol glycine equivalents/g live wt. infection with schistosoma mansoni, however, ...19827172633
acquired resistance in snails. induction of resistance to schistosoma mansoni in biomphalaria glabrata. 19836874229
schistosoma mansoni: immunofluorescent detection of its antigen reacting with biomphalaria glabrata amoebocytes.the reaction of amoebocytes in the hemolymph of the infected intermediate host, biomphalaria glabrata, to schistosoma mansoni antigens has been investigated using the indirect immunofluorescence antibody test. monolayers of amoebocytes, prepared from hemolymph of infected and normal snails, were first fixed and then reacted with antisera obtained from mice infected for 7 to 9 weeks. nonspecific and cross-reactions between the antisera and monolayers of amoebocytes were eliminated by absorbing th ...19836337058
altered feeding response as a cause for the altered heartbeat rate and locomotor activity of schistosoma mansoni-infected biomphalaria glabrata.biomphalaria glabrata infected with schistosoma mansoni for 33 days fed more often than uninfected snails. whereas uninfected snails had nocturnal increases in feeding, snails with a 33-day-old infection of s. mansoni fed as often during the day as in the night. using direct observation and film analysis, we found that feeding increased the heartbeat rate and locomotor activity of b. glabrata. when snails were allowed to feed ad lib., infected snails had higher heartbeat rates than uninfected sn ...19836631636
effects of alterations in the heartbeat rate and locomotor activity of schistosoma mansoni-infected biomphalaria glabrata on cercarial emergence.during a 27-hr period of food deprivation, biomphalaria glabrata infected with schistosoma mansoni had lower heartbeat rates and locomotor activities than did controls that were feeding ad lib. however, there was no difference between the cercarial emergence from control and experimental snails either before, during, or after the period of food deprivation. no correlation was found between the locomotor activity of the snail and the number of cercariae emerging from the snail in continuous light ...19836631637
purine metabolism in biomphalaria glabrata under starvation and infection with schistosoma mansoni.purine content and its alterations during a starvation period of 29 days and an infection period of 52 days were studied. during starvation the amount of uric acid increased from 2.11 +/- 0.95 mg/g dry wt in control snails to a maximum of 6.73 +/- 3.99 mg/g dry wt on day 29 of starvation for uric acid and from 4.73 +/- 2.10 mg/g dry wt in control snails to a maximum of 11.29 +/- 3.48 mg/g dry wt on day 23 of starvation for guanine. during infection there was an increase with both uric acid and g ...19836641159
mimicry of snail host antigens by miracidia and primary sporocysts of schistosoma mansoni.polyvalent antisera generated in rabbits to soluble haemolymph components from schistosoma mansoni-susceptible (pr albino 'm line') and s. mansoni-resistant (10-r2) stocks of the snail biomphalaria glabrata were employed as membrane probes to determine if antigens related to snail haemolymph were produced by the early larval stages of s. mansoni (pr-1 strain). using immunofluorescence and immunoelectron microscopical methods we have demonstrated that antibodies to susceptible (anti-suscept) and ...19836191268
schistosoma mansoni and biomphalaria glabrata share epitopes: antibodies to sporocysts bind host snail hemocytes. 19836195272
displacement of biomphalaria glabrata by the snail thiara granifera in field habitats in st. lucia, west indies.thiara granifera is a melaniid snail capable of maintaining very high densities in a variety of habitats. it has been introduced into the new world from the far east and is now spreading rapidly throughout the caribbean. in puerto rico and dominica casual observations following natural invasion by t. granifera suggest that it may exert a powerful restraining influence on populations of biomphalaria glabrata, the major intermediate host of schistosoma mansoni in the caribbean. the potential of t. ...19836882056
laboratory comparison of the relative success of biomphalaria glabrata stocks which are susceptible and insusceptible to infection with schistosoma mansoni.a method of interrupting the life-cycle of the human blood fluke schistosoma by increasing the proportion of genetically insusceptible intermediate host snails in natural populations was first proposed nearly 25 years ago. the method assumes that insusceptible snails will be at a selective advantage over susceptible snails when the schistosome parasite is present, and therefore natural selection will act to increase the proportion of alleles for insusceptibility. a major objection to the propose ...19836856336
[experimental infection of biomphalaria glabrata with schistosoma mansoni by the injection of miracidia]. 19836672864
lysosomal enzyme activities in susceptible and refractory strains of biomphalaria glabrata during the course of infection with schistosoma mansoni.the distribution and abundance of the lysosomal enzyme markers, acid phosphatase (ap), peroxidase (po), and nonspecific esterase (ne), within circulating blood cells (hemocytes) were examined in a schistosome-susceptible (pr albino m-line) and a resistant (10-r2) strain of biomphalaria glabrata during the course of infection with schistosoma mansoni. the dynamics of serum (cell-free hemolymph) ap activities and total hemocyte numbers in infected snails also were investigated. hemocyte subpopulat ...19836674450
schistosoma mansoni: long-term maintenance of clones by microsurgical transplantation of sporocysts.schistosoma mansoni sporocysts originally derived from monomiracidially infected biomphalaria glabrata snails were serially transplanted into the cephalopedal sinus of anesthetized snails by the microsurgical implantation of fragments of parasitized hepatopancreas and ovotestis. three to six passages each of five male and five female clones were maintained for as long as 2.0 years. of the recipient snails which survived surgery, 87% released cercariae, usually beginning 5-7 weeks after surgery. ...19846692880
the adenylate nucleotide pool in the digestive gland-gonad complex of biomphalaria glabrata infected by schistosoma mansoni.the levels of adenylate nucleotides were examined in the digestive gland and ovotestes of biomphalaria glabrata during cercarial shedding of schistosoma mansoni, 10 weeks post-infection. in general, parasitization resulted in decreases in the adenylate levels in both tissues, but the results were not statistically significant. moreover, the energy charge ratio was not significantly altered. the mean energy charge in ovotestes from uninfected and infected individuals was 0.81 and 0.77, respective ...19846527694
[new foci of transmission of schistosoma mansoni in the state of pará].two new foci of transmission of schistosoma mansoni in the state of pará are recorded, with the finding of naturally infected biomphalaria glabrata in the municipalities of viseu and belém. uninfected specimens of biomphalaria straminea, as well as the planorbid species biomphalaria schrammi, drepanotrema lucidum and d. anatinum, were found in the same area.19846535922
[chemoprophylaxis of schistosomiasis: molluscacidal activity of natural products--assays with adult snails and oviposition].data concerning the molluscicidal activity of 159 extracts from 84 brazilian plants on biomphalaria glabrata, the most important intermediate host of schistosoma mansoni in brazil, are presented. seventy eight extracts (49,0%) showed activity against snails or eggs, but only twenty nine (18.2%) were active on both, snails and eggs. extracts of two species (mikania hirsutissima and qualea multiflora) have shown to be lethals to adult snails at 10 ppm concentration.19846548884
role of cellular and humoral components in the encapsulation response of biomphalaria glabrata to schistosoma mansoni sporocysts in vitro. 19846483876
isolation, characterization and functional assessment of a hemagglutinin from the plasma of biomphalaria glabrata, intermediate host of schistosoma mansoni.an agglutinin from the plasma of biomphalaria glabrata, vector of schistosoma mansoni, has been isolated and partially characterized. its isolation is a simple two-step process, yielding an essentially pure preparation that can then be manipulated free from other plasma components. it is a large glycoprotein (10(6) daltons), found in four of the five snail strains tested, and possesses affinity for galactose-type sugar residues. based upon criteria such as molecular weight, carbohydrate and eryt ...19846500135
chronobiological aspects of the host-parasite relationships between biomphalaria glabrata and schistosoma mansoni: cercarial production and infectivity, and growth kinetics of the host. 19846501917
[experimental schistosomiasis iii. observations on the infestation of biomphalaria glabrata by a miracidium of schistosoma mansoni].380 b. glabrata infected with 1 miracidium of s. mansoni produced 87 positive snails (22,9%), 38 of which sending out male cercariae and 49 female cercariae. the snails belonged to 3 experimental series kept at 27 degrees and 24 degrees c for 15 weeks under continuous lighting. there was no difference between the proportions of both sexes. the survival of the positive snails was favoured by the male sex. the shedding of female cercariae was greater than shedding of male. during those 15 weeks, t ...19846721371
schistosoma mansoni: agglutination of sporocysts, and formation of gels on miracidia transforming in plasma of biomphalaria glabrata.the resistance or susceptibility of biomphalaria glabrata strains to strains of schistosoma mansoni, the human blood fluke, are evidenced by the responses of snail hemocytes to sporocysts of the schistosome, both in vivo and in vitro. it is now reported that living sporocysts of the pr1 strain of s. mansoni agglutinate in the plasma of all tested strains of b. glabrata, in contrast to fixed sporocysts which agglutinate only in plasma from resistant snail strains. the agglutinating activity in re ...19846745386
intergeneric reproductive stimulation and parthenogenesis in schistosoma mansoni.when mice were infected with female cercariae of schistosoma mansoni and male cercariae of schistosomatium douthitti, many mixed pairs formed. the paired females were approximately the same size as those in unisexual infections, far smaller than females paired with s. mansoni males. although the sch. douthitti males possessed well-developed testes, sperm were not found in their female partners, which developed scanty vitelline glands and produced laterally spined eggs typical of s. mansoni. such ...19846504564
[activities of a schistosoma mansoni focus of transmission in martinique (french antilles)].a focus of intestinal schistosomiasis was discovered in november 1981 in martinique in the saint-pierre area. an analysis of its structure and of the mecanisms of the transmission of the parasite was carried out between 1981 and 1983. this focus presents the following particularities: a transmission site very small constituted by water cress beds; a small human population infected with a prevalence of 13% (positive stools) to 41,3% (positive serologies); a weak fecal contamination of the water; ...19846524823
[selection of genotypes of schistosoma mansoni and their maintenance by sporocyst transplantation].schistosoma mansoni was isolated by hatching eggs obtained from a naturally infected rat in grand etang, guadeloupe; fifty biomphalaria glabrata were exposed to five miracidia each. the resulting cercariae were used to infect laboratory mice which were later sacrificed to provide worms for enzyme analyses and eggs for further infections. seven enzymes in extracts of individual worms were examined by isoelectric focusing in polyacrylamide gels: acp, g6pdh, pgm, gpi and hk showed no variation, whe ...19846437612
an ultrastructural study of the effect of parasitism by larval schistosoma mansoni on the calcium reserves of the host, biomphalaria glabrata.the tissues of biomphalaria glabrata contain three types of calcium cells which can be differentiated by their location and the size and number of their contained corpuscles. x-ray analysis has confirmed the presence of calcium and magnesium in the corpuscles. molluscs containing the larval stages of schistosoma mansoni at 40 days post infection show disintegration of the calcareous corpuscles in type-a calcium cells and erosion of the inner surface of the shell.19846467339
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