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long-lasting insecticide-treated house screens and targeted treatment of productive breeding-sites for dengue vector control in acapulco, mexico.long-lasting insecticidal net screens (llis) fitted to domestic windows and doors in combination with targeted treatment (tt) of the most productive aedes aegypti breeding sites were evaluated for their impact on dengue vector indices in a cluster-randomised trial in mexico between 2011 and 2013.025604761
entomological impact and social participation in dengue control: a cluster randomized trial in fortaleza, brazil.this study intended to implement a novel intervention strategy, in brazil, using an ecohealth approach and analyse its effectiveness and costs in reducing aedes aegypti vector density as well as its acceptance, feasibility and sustainability. the intervention was conducted from 2012 to 2013 in the municipality of fortaleza, northeast brazil.025604760
improved dengue fever prevention through innovative intervention methods in the city of salto, uruguay.uruguay is located at the southern border of aedes aegypti distribution on the south american sub-continent. the reported dengue cases in the country are all imported from surrounding countries. one of the cities at higher risk of local dengue transmission is salto, a border city with heavy traffic from dengue endemic areas.025604764
disruption of dengue virus transmission by mosquitoes.current control efforts for mosquito-borne arboviruses focus on mosquito control involving insecticide applications, which are becoming increasingly ineffective and unsustainable in urban areas. mosquito population replacement is an alternative arbovirus control concept aiming at replacing virus-competent vector populations with laboratory-engineered incompetent vectors. a prerequisite for this strategy is the design of robust anti-pathogen effectors that can ultimately be genetically driven thr ...026120563
pyrethroid-resistance and presence of two knockdown resistance (kdr) mutations, f1534c and a novel mutation t1520i, in indian aedes aegypti.control of aedes aegypti, the mosquito vector of dengue, chikungunya and yellow fever, is a challenging task. pyrethroid insecticides have emerged as a preferred choice for vector control but are threatened by the emergence of resistance. the present study reports a focus of pyrethroid resistance and presence of two kdr mutations--f1534c and a novel mutation t1520i, in ae. aegypti from delhi, india.201525569164
local evolution of pyrethroid resistance offsets gene flow among aedes aegypti collections in yucatan state, mexico.the mosquito aedes aegypti is the major vector of the four serotypes of dengue virus (denv1-4). previous studies have shown that ae. aegypti in mexico have a high effective migration rate and that gene flow occurs among populations that are up to 150 km apart. since 2000, pyrethroids have been widely used for suppression of ae. aegypti in cities in mexico. in yucatan state in particular, pyrethroids have been applied in and around dengue case households creating an opportunity for local selectio ...201425371186
evaluation of aadop2 receptor antagonists reveals antidepressants and antipsychotics as novel lead molecules for control of the yellow fever mosquito, aedes aegypti.the yellow fever mosquito, aedes aegypti, vectors disease-causing agents that adversely affect human health, most notably the viruses causing dengue and yellow fever. the efficacy of current mosquito control programs is challenged by the emergence of insecticide-resistant mosquito populations, suggesting an urgent need for the development of chemical insecticides with new mechanisms of action. one recently identified potential insecticide target is the a. aegypti d1-like dopamine receptor, aadop ...201425332454
aedes aegypti and other mosquitos in relation to the dengue syndrome. 04383543
reduced competitiveness of wolbachia infected aedes aegypti larvae in intra- and inter-specific immature interactions.wolbachia are maternally inherited intracellular bacteria that frequently infect a diverse range of arthropod species. empirical and theoretical studies examining wolbachia invasiveness have emphasized wolbachia effects on adult hosts, but recent studies show that wolbachia impacts on immature hosts can be important also. here, we have examined for effects of wolbachia infection in aedes aegypti. specifically, differential survivorship is observed when young larvae (1st instar) are exposed to ol ...201323933013
an improved autocidal gravid ovitrap for the control and surveillance of aedes aegypti.limited success has been achieved using traditional vector control methods to prevent the transmission of dengue viruses. integrated control programs incorporating alternative tools, such as gravid ovitraps (lethal ovitraps and sticky ovitraps) may provide greater potential for monitoring and reducing vector populations and dengue virus transmission. we had developed an autocidal gravid ovitrap (ago) as a simple, low-cost device for surveillance and control of ae. aegypti without the use of pest ...201323919568
genetic mapping of specific interactions between aedes aegypti mosquitoes and dengue viruses.specific interactions between host genotypes and pathogen genotypes (g×g interactions) are commonly observed in invertebrate systems. such specificity challenges our current understanding of invertebrate defenses against pathogens because it contrasts the limited discriminatory power of known invertebrate immune responses. lack of a mechanistic explanation, however, has questioned the nature of host factors underlying g×g interactions. in this study, we aimed to determine whether g×g interaction ...201323935524
molecular and functional characterization of odorant-binding protein genes in an invasive vector mosquito, aedes albopictus.aedes albopictus is a major vector of dengue and chikungunya viruses. olfaction plays a vital role in guiding mosquito behaviors and contributes to their ability to transmit pathogens. odorant-binding proteins (obps) are abundant in insect olfactory tissues and involved in the first step of odorant reception. while comprehensive descriptions are available of obps from aedes aegypti, culex quinquefasciatus and anopheles gambiae, only a few genes from ae. albopictus have been reported. in this stu ...201323935894
body size and wing shape measurements as quality indicators of aedes aegypti mosquitoes destined for field release.there is increasing interest in rearing modified mosquitoes for mass release to control vector-borne diseases, particularly wolbachia-infected aedes aegypti for suppression of dengue. successful introductions require release of high quality mosquitoes into natural populations. potential indicators of quality are body size and shape. we tested to determine if size, wing/thorax ratio, and wing shape are associated with field fitness of wolbachia-infected ae. aegypti. compared with field-collected ...201323716403
sustained reduction of the dengue vector population resulting from an integrated control strategy applied in two brazilian cities.aedes aegypti has developed evolution-driven adaptations for surviving in the domestic human habitat. several trap models have been designed considering these strategies and tested for monitoring this efficient vector of dengue. here, we report a real-scale evaluation of a system for monitoring and controlling mosquito populations based on egg sampling coupled with geographic information systems technology. the smcp-aedes, a system based on open technology and open data standards, was set up fro ...201323844059
molecular evidence for dual pyrethroid-receptor sites on a mosquito sodium channel.pyrethroid insecticides are widely used as one of the most effective control measures in the global fight against agricultural arthropod pests and mosquito-borne diseases, including malaria and dengue. they exert toxic effects by altering the function of voltage-gated sodium channels, which are essential for proper electrical signaling in the nervous system. a major threat to the sustained use of pyrethroids for vector control is the emergence of mosquito resistance to pyrethroids worldwide. her ...201323821746
the effect of virus-blocking wolbachia on male competitiveness of the dengue vector mosquito, aedes aegypti.the bacterial endosymbiont wolbachia blocks the transmission of dengue virus by its vector mosquito aedes aegypti, and is currently being evaluated for control of dengue outbreaks. wolbachia induces cytoplasmic incompatibility (ci) that results in the developmental failure of offspring in the cross between wolbachia-infected males and uninfected females. this increases the relative success of infected females in the population, thereby enhancing the spread of the beneficial bacterium. however, w ...201425502564
she's a femme fatale: low-density larval development produces good disease vectors.two hypotheses for how conditions for larval mosquitoes affect vectorial capacity make opposite predictions about the relationship of adult size and frequency of infection with vector-borne pathogens. competition among larvae produces small adult females. the competition-susceptibility hypothesis postulates that small females are more susceptible to infection and predicts frequency of infection should decrease with size. the competition-longevity hypothesis postulates that small females have low ...025591112
promoting health education and public awareness about dengue and its mosquito vector in saudi arabia.currently, dengue fever is considered as the main health problem in several parts (mekkah, jeddah, jazan and najran) of kingdom of saudi arabia (ksa) with dramatically increase in the number of cases reported every year. this is associated with obvious ineffectiveness in the recent control and management programs for the mosquito vector (aedes aegypti). here, we suggested promoting the health education and public awareness among saudi people to improve the control of dengue mosquito vector. seve ...201425403705
evolution of mosquito preference for humans linked to an odorant receptor.female mosquitoes are major vectors of human disease and the most dangerous are those that preferentially bite humans. a 'domestic' form of the mosquito aedes aegypti has evolved to specialize in biting humans and is the main worldwide vector of dengue, yellow fever, and chikungunya viruses. the domestic form coexists with an ancestral, 'forest' form that prefers to bite non-human animals and is found along the coast of kenya. we collected the two forms, established laboratory colonies, and docu ...025391959
discovery and characterization of a potent and selective inhibitor of aedes aegypti inward rectifier potassium channels.vector-borne diseases such as dengue fever and malaria, which are transmitted by infected female mosquitoes, affect nearly half of the world's population. the emergence of insecticide-resistant mosquito populations is reducing the effectiveness of conventional insecticides and threatening current vector control strategies, which has created an urgent need to identify new molecular targets against which novel classes of insecticides can be developed. we previously demonstrated that small molecule ...201425375326
a social-ecological analysis of community perceptions of dengue fever and aedes aegypti in machala, ecuador.the growing burden of dengue fever and the lack of a vaccine or specific medical treatment have increased the urgency of the public health sector to identify alternative management strategies. a prevailing trend in latin america has been a shift towards decentralized vector control programs with integrated management strategies, requiring significant intersectoral coordination, community engagement, and knowledge of the local social-ecological system (ses). community perceptions and responses ar ...201425370883
estimation of aedes aegypti (diptera: culicidae) population size and adult male survival in an urban area in panama.traditional mosquito control strategies rely heavily on the use of chemical insecticides. however, concerns about the efficiency of traditional control methods, environmental impact and emerging pesticide resistance have highlighted the necessity for developing innovative tools for mosquito control. some novel strategies, including release of insects carrying a dominant lethal gene (ridl®), rely on the sustained release of modified male mosquitoes and therefore benefit from a thorough understand ...201425410991
the dengue virus mosquito vector aedes aegypti at high elevation in mexico.méxico has cities (e.g., méxico city and puebla city) located at elevations > 2,000 m and above the elevation ceiling below which local climates allow the dengue virus mosquito vector aedes aegypti to proliferate. climate warming could raise this ceiling and place high-elevation cities at risk for dengue virus transmission. to assess the elevation ceiling for ae. aegypti and determine the potential for using weather/climate parameters to predict mosquito abundance, we surveyed 12 communities alo ...201222987656
need for an efficient adult trap for the surveillance of dengue vectors.the emergence and re-emergence of arboviral diseases transmitted by aedes aegypti and ae. albopictus continue to be a major threat in the tropics and subtropics. associations between currently used indices and dengue transmission have not been proven to be satisfactorily predictive of dengue epidemics. classical larval indices in dengue surveillance have limited use in assessing transmission risk and are a poor proxy for measuring adult emergence. besides, collection of larval indices is labour ...023287120
gene flow networks among american aedes aegypti populations.the mosquito aedes aegypti, the dengue virus vector, has spread throughout the tropics in historical times. while this suggests man-mediated dispersal, estimating contemporary connectivity among populations has remained elusive. here, we use a large mtdna dataset and a bayesian coalescent framework to test a set of hypotheses about gene flow among american ae. aegypti populations. we assessed gene flow patterns at the continental and subregional (amazon basin) scales. for the americas, our data ...023144654
dengue vector management using insecticide treated materials and targeted interventions on productive breeding-sites in guatemala.in view of the epidemiological expansion of dengue worldwide and the availability of new tools and strategies particularly for controlling the primary dengue vector aedes aegypti, an intervention study was set up to test the efficacy, cost and feasibility of a combined approach of insecticide treated materials (itms) alone and in combination with appropriate targeted interventions of the most productive vector breeding-sites.201223110515
insecticide susceptibility of aedes aegypti populations from senegal and cape verde archipelago.two concomitant dengue 3 (den-3) epidemics occurred in cape verde archipelago and senegal between september and october 2009. aedes aegypti was identified as the vector of these epidemics as several den-3 virus strains were isolated from this species in both countries. the susceptibility to pyrethroids, organochlorine, organophosphates and carbamate was investigated in two field strains of aedes aegypti from both countries using who diagnostic bioassay kits in order to monitor their the current ...201223088621
aging field collected aedes aegypti to determine their capacity for dengue transmission in the southwestern united states.aedes aegypti, the primary vector of dengue virus, is well established throughout urban areas of the southwestern us, including tucson, az. local transmission of the dengue virus, however, has not been reported in this area. although many factors influence the distribution of the dengue virus, we hypothesize that one contributing factor is that the lifespan of female ae. aegypti mosquitoes in the southwestern us is too short for the virus to complete development and be transmitted to a new host. ...201223077536
analyzing the spatio-temporal relationship between dengue vector larval density and land-use using factor analysis and spatial ring mapping.dengue, a mosquito-borne febrile viral disease, is found in tropical and sub-tropical regions and is now extending its range to temperate regions. the spread of the dengue viruses mainly depends on vector population (aedes aegypti and aedes albopictus), which is influenced by changing climatic conditions and various land-use/land-cover types. spatial display of the relationship between dengue vector density and land-cover types is required to describe a near-future viral outbreak scenario. this ...201223043443
seasonal population dynamics and the genetic structure of the mosquito vector aedes aegypti in são paulo, brazil.population genetic studies of insect vectors can generate knowledge to improve epidemiological studies focused on the decrease of pathogen transmission. in this study, we used nine snps across the aedes aegypti genome to characterize seasonal population variations of this important dengue vector. mosquito samples were obtained by ovitraps placed over botucatu sp from 2005 to 2010. our data show that, regardless of the large variation in mosquito abundance (deduced from the number of eggs obtaine ...201223170214
identifying the effective concentration for spatial repellency of the dengue vector aedes aegypti.current efforts are underway to quantify the chemical concentration in a treated air space that elicits a spatial repellent (deterrent) response in a vector population. such information will facilitate identifying the optimum active ingredient (ai) dosage and intervention coverage important for the development of spatial repellent tools--one of several novel strategies being evaluated for vector-borne disease control. this study reports initial findings from air sampling experiments conducted un ...201223273133
assessing the feasibility of controlling aedes aegypti with transgenic methods: a model-based evaluation.suppression of dengue and malaria through releases of genetically engineered mosquitoes might soon become feasible. aedes aegypti mosquitoes carrying a conditionally lethal transgene have recently been used to suppress local vector populations in small-scale field releases. prior to releases of transgenic insects on a wider scale, however, most regulatory authorities will require additional evidence that suppression will be effective in natural heterogeneous habitats. we use a spatially explicit ...201223284949
pyrethroid resistance in aedes aegypti and aedes albopictus from port-au-prince, haiti.in port-au-prince, haiti, the status of insecticide resistance has not recently been evaluated for aedes aegypti (l) and aedes albopictus (skuse) populations. no prophylactics exist for dengue, so prevention is only through vector control methods. an earthquake occurred in haiti on january 12, 2010, with a magnitude of 7.0 mw that devastated the area. dengue became a major concern for the humanitarian relief workers that entered the country. bottle bioassays were conducted in the field on adult ...023181855
community-based control of aedes aegypti by adoption of eco-health methods in chennai city, india.dengue is highly endemic in chennai city, south india, in spite of continuous vector control efforts. this intervention study was aimed at establishing the efficacy as well as the favouring and limiting factors relating to a community-based environmental intervention package to control the dengue vector aedes aegypti.023318241
estimating dengue vector abundance in the wet and dry season: implications for targeted vector control in urban and peri-urban asia.research has shown that the classical stegomyia indices (or "larval indices") of the dengue vector aedes aegypti reflect the absence or presence of the vector but do not provide accurate measures of adult mosquito density. in contrast, pupal indices as collected in pupal productivity surveys are a much better proxy indicator for adult vector abundance. however, it is unknown when it is most optimal to conduct pupal productivity surveys, in the wet or in the dry season or in both, to inform contr ...023318235
insecticide resistance in the dengue vector aedes aegypti from martinique: distribution, mechanisms and relations with environmental factors.dengue is an important mosquito borne viral disease in martinique island (french west indies). the viruses responsible for dengue are transmitted by aedes aegypti, an indoor day-biting mosquito. the most effective proven method for disease prevention has been by vector control by various chemical or biological means. unfortunately insecticide resistance has already been observed on the island and recently showed to significantly reduce the efficacy of vector control interventions. in this study, ...201222363529
changing domesticity of aedes aegypti in northern peninsular malaysia: reproductive consequences and potential epidemiological implications.the domestic dengue vector aedes aegypti mosquitoes breed in indoor containers. however, in northern peninsular malaysia, they show equal preference for breeding in both indoor and outdoor habitats. to evaluate the epidemiological implications of this peridomestic adaptation, we examined whether ae. aegypti exhibits decreased survival, gonotrophic activity, and fecundity due to lack of host availability and the changing breeding behavior.201222363516
climate-based models for understanding and forecasting dengue epidemics.dengue dynamics are driven by complex interactions between human-hosts, mosquito-vectors and viruses that are influenced by environmental and climatic factors. the objectives of this study were to analyze and model the relationships between climate, aedes aegypti vectors and dengue outbreaks in noumea (new caledonia), and to provide an early warning system.201222348154
genetic mapping a meiotic driver that causes sex ratio distortion in the mosquito aedes aegypti.an endogenous meiotic driver in the dengue and yellow fever vector mosquito aedes aegypti can cause highly male-biased sex ratio distortion in crosses from suitable genetic backgrounds. we previously selected a strain that carries a strong meiotic drive gene (d) linked with the male-determining allele (m) on chromosome 1 in a. aegypti. here, we performed segregation analysis of the m(d) locus among backcross (bc(1)) progeny from a driver male and drive-sensitive females. assessment of sex ratios ...201222308303
dengue antibodies in blood donors.dengue is an urban arbovirus whose etiologic agent is a virus of the genus flavorius with four distinct antigen serotypes (denv-1, denv-2, denv-3 and denv-4) that is transmitted to humans through the bite of the mosquito aedes aegypti. the campo mourão region in brazil is endemic for dengue fever. obtective: the aim of this study was to evaluate the presence of igg and igm antibodies specific to the four serotypes of dengue in donors of the blood donor service in the city of campo mourão.023049418
strain variation in the transcriptome of the dengue fever vector, aedes aegypti.studies of transcriptome dynamics provide a basis for understanding functional elements of the genome and the complexity of gene regulation. the dengue vector mosquito, aedes aegypti, exhibits great adaptability to diverse ecological conditions, is phenotypically polymorphic, and shows variation in vectorial capacity to arboviruses. previous genome sequencing showed richness in repetitive dna and transposable elements that can contribute to genome plasticity. population genetic studies revealed ...201222384387
dengue, urbanization and globalization: the unholy trinity of the 21(st) century.dengue is the most important arboviral disease of humans with over half of the world's population living in areas of risk. the frequency and magnitude of epidemic dengue have increased dramatically in the past 40 years as the viruses and the mosquito vectors have both expanded geographically in the tropical regions of the world. there are many factors that have contributed to this emergence of epidemic dengue, but only three have been the principal drivers: 1) urbanization, 2) globalization and ...201122500131
the value of educational messages embedded in a community-based approach to combat dengue fever: a systematic review and meta regression analysis.the effects of various dengue control measures have been investigated in previous studies. the aim of this review was to investigate the relative effectiveness (re) of different educational messages embedded in a community-based approach on the incidence of aedes aegypti larvae using entomological measures as outcomes.201121886848
male-female differences in the number of reported incident dengue fever cases in six asian countries.demographic factors, such as age and sex, are associated with the likelihood of exposure to aedes aegypti, the vector for dengue. however, dengue data disaggregated by both sex and age are not routinely reported or analysed by national surveillance systems. this study analysed the reported number of incident dengue cases by age and sex for six countries in asia.201123908884
prevalence and insecticide susceptibility of dengue vectors in the district of batticaloa in eastern sri lanka.unprecedented incidences of dengue have been reported in sri lanka in recent years. the district of batticaloa, which was devastated by the 2004 asian tsunami, is one of the districts affected by dengue. one option to curtail this disease is to implement appropriate vector control measures. a nine-month study was carried out within the batticaloa municipal council limit from april to december 2008. larval collections were conducted fortnightly using conventional ovitraps for nine months covering ...201122028610
application of post-pcr methods for analysis of mosquito densovirus.two clades of aedes densovirus, aedes aegypti densovirus and aedes albopictus densovirus, were classified according to the origin of isolation. these two densoviruses were isolated from indigenous mosquitoes and mosquito cell lines, respectively. this group of invertebrate viruses belongs to the subfamily densovirinae of the parvoviridae family and infects only insects. several types of densoviruses have been isolated from mosquitoes especially aedes aegypti and aedes albopictus, which are impor ...201425427347
comparison of mosquito densoviruses: two clades of viruses isolated from indigenous mosquitoes.we analyzed the phylogenetic tree of densoviruses isolated from indigenous mosquitoes and mosquito cell lines. our findings suggest two distinct clades of densovirus. the viruses in the first clade were isolated from an indigenous mosquito which had the aedes aegypti densovirus (aaednv) as a representative virus. the other clade of viruses was isolated from mosquito indigenous cell line which had the aedes albopictus densovirus (aaldnv) as the representative virus. the origin of the two clades o ...201324050091
new genetic variation of aedes albopictus densovirus isolated from mosquito c6/36 cell line.densovirus (dnv) is a small single-stranded dna, non-enveloped virus belonging to the subfamily densovirinae of the parvoviridae family. this group of invertebrate viruses infects exclusively insects. two of the major densoviruses, aedes aegypti (aaednv) and ae. albopictus (aaldnv), infect mosquitoes that carry viruses responsible for two important public health diseases, namely, dengue hemorrhagic fever and yellow fever. the present study describes cloning, sequencing and phylogenetic analysis ...201223431818
green synthesis of silver nanoparticles using delphinium denudatum root extract exhibits antibacterial and mosquito larvicidal activities.green synthesis of silver nanoparticles (agnps) using aqueous root extract of delphinium denudatum (dd) by reduction of ag(+) ions from silver nitrate solution has been investigated. the synthesized ddagnps were characterized by using uv-vis spectroscopy, x-ray diffraction (xrd), field emission scanning electron microscope (fesem) and fourier transform infrared spectroscopy (ftir). the prepared ddagnps showed maximum absorbance at 416nm and particles were polydispersed in nature, spherical in sh ...201424632157
extracellular synthesis of silver nanoparticles using bacillus megaterium against malarial and dengue vector (diptera: culicidae).biosynthesis of silver nanoparticles has provoked nowadays and alternative to physical and chemical approaches. in the present study, silver nanoparticles (agnps) were synthesized extracellular method using bacillus megaterium. the agnps formations were confirmed initially through color change, and the aliquots were characterized through uv-visible spectrophotometer, followed by scanning electron microscopy (sem), energy-dispersive x-ray (edx) spectroscopy, and fourier transform infrared (ftir) ...201526231835
a large scale biorational approach using bacillus thuringiensis israeliensis (strain am65-52) for managing aedes aegypti populations to prevent dengue, chikungunya and zika transmission.aedes aegypti is a container-inhabiting mosquito and a vector of dengue, chikungunya, and zika viruses. in 2009 several cases of autochthonous dengue transmission were reported in key west, florida, usa prompting a comprehensive response to control a. aegypti. in key west, larvae of this mosquito develop in containers around human habitations which can be numerous and labor intensive to find and treat. aerial applications of larvicide covering large areas in a short time can be an efficient and ...201728199323
levels of insecticide resistance to deltamethrin, malathion, and temephos, and associated mechanisms in aedes aegypti mosquitoes from the guadeloupe and saint martin islands (french west indies).in the guadeloupe and saint martin islands, aedes aegypti mosquitoes are the only recognized vectors of dengue, chikungunya, and zika viruses. for around 40 years, malathion was used as a mosquito adulticide and temephos as a larvicide. since the european union banned the use of these two insecticide molecules in the first decade of the 21st century, deltamethrin and bacillus thuringiensis var. israelensis are the remaining adulticide and larvicide, respectively, used in guadeloupe. in order to ...201728187780
local mosquito-borne transmission of zika virus - miami-dade and broward counties, florida, june-august 2016.during the first 6 months of 2016, large outbreaks of zika virus disease caused by local mosquito-borne transmission occurred in puerto rico and other u.s. territories, but local mosquito-borne transmission was not identified in the continental united states (1,2). as of july 22, 2016, the florida department of health had identified 321 zika virus disease cases among florida residents and visitors, all occurring in either travelers from other countries or territories with ongoing zika virus tran ...201627684886
receptors are affected by selection with each bacillus thuringiensis israelensis cry toxin but not with the full bti mixture in aedes aegypti.bacillus thuringiensis israelensis (bti) toxins are increasingly used for mosquito control, but little is known about the precise mode of action of each of these toxins, and how they interact to kill mosquito larvae. by using rna sequencing, we investigated change in gene transcription level and polymorphism variations associated with resistance to each bti cry toxin and to the full bti toxin mixture in the dengue vector aedes aegypti. the up-regulation of genes related to chitin metabolism in a ...201627418233
the use of aedes aegypti larvae attractants to enhance the effectiveness of larvicides.aedes aegypti (l.) is an important dengue, chikungunya, and yellow fever vector. immature stages of this species inhabit human-made containers placed in residential landscapes, and the application of larvicides inside containers that cannot be eliminated is still considered a priority in control programs. larvicidal efficacy is influenced by several factors, including the formulation used, the water quality, and the susceptibility of larvae, among others. if an attractant can be incorporated int ...201626922177
evaluating liquid and granular bacillus thuringiensis var. israelensis broadcast applications for controlling vectors of dengue and chikungunya viruses in artificial containers and tree holes.the principal vectors of chikungunya and dengue viruses typically oviposit in water-filled artificial and natural containers, including tree holes. despite the risk these and similar tree hole-inhabiting mosquitoes present to global public health, surprisingly few studies have been conducted to determine an efficient method of applying larvicides specifically to tree holes. the stihl sr 450, a backpack sprayer commonly utilized during military and civilian vector control operations, may be suita ...201526335473
susceptibility profile of aedes aegypti from santiago island, cabo verde, to insecticides.in 2009, cabo verde diagnosed the first dengue cases, with 21,137 cases reported and aedes aegypti was identified as the vector. since the outbreak, chemical insecticides and source reduction were used to control the mosquito population. this study aimed to assess the susceptibility of a. aegypti populations from santiago, cabo verde to insecticides and identify the mechanisms of resistance. samples of a. aegypti eggs were obtained at two different time periods (2012 and 2014), using ovitraps in ...201526307496
the genetic architecture of a complex trait: resistance to multiple toxins produced by bacillus thuringiensis israelensis in the dengue and yellow fever vector, the mosquito aedes aegypti.the bacterial insecticide bacillus thuringiensis subsp. israelensis (bti) is an increasingly popular alternative to chemical insecticides for controlling mosquito populations. because bti toxicity relies on the action of four main toxins, resistance to bti is very likely a complex phenotype involving several genes simultaneously. dissecting the underlying genetic basis thus requires associating a quantitative measure of resistance to genetic variation at many loci in a segregating population. he ...201526238211
cry4ba and cyt1aa proteins from bacillus thuringiensis israelensis: interactions and toxicity mechanism against aedes aegypti.individual crystal proteins from bacillus thuringiensis israelensis exhibit variable levels of insecticidal activities against mosquito larvae. in all cases, they are much less active compared to the whole crystal proteins due to described complex synergistic interactions among them. in the present study we investigated the effects of cyt1a98 (a cyt1aa type protein) on cry4blb (a cry4ba type toxin) insecticidal activity toward the dengue vector aedes aegypti. the bioassay analyses demonstrated t ...201526238170
biosynthesis of silver nanoparticles using bacillus thuringiensis against dengue vector, aedes aegypti (diptera: culicidae).the present study reveals the larvicidal activity of silver nanoparticles (agnps) synthesized by bacillus thuringiensis (bt) against aedes aegypti responsible for the diseases of public health importance. the bt-agnps were characterized by using uv-visible spectrophotometer followed by scanning electron microscopy (sem) and energy-dispersive x-ray (edx) spectroscopy. a surface plasmon resonance spectrum of agnps was obtained at 420 nm. the particle sizes were measured through sem imaging ranging ...201424173811
eco-friendly microbial route to synthesize cobalt nanoparticles using bacillus thuringiensis against malaria and dengue vectors.the developments of resistance and persistence to chemical insecticides and concerns about the non-target effects have prompted the development of eco-friendly mosquito control agents. the aim of this study was to investigate the larvicidal activities of synthesized cobalt nanoparticles (co nps) using bio control agent, bacillus thuringiensis against malaria vector, anopheles subpictus and dengue vector, aedes aegypti (diptera: culicidae). the synthesized co nps were characterized by x-ray diffr ...201324013343
contrasting patterns of tolerance between chemical and biological insecticides in mosquitoes exposed to uv-a.mosquitoes are vectors of major human diseases, such as malaria, dengue or yellow fever. because no efficient treatments or vaccines are available for most of these diseases, control measures rely mainly on reducing mosquito populations by the use of insecticides. numerous biotic and abiotic factors are known to modulate the efficacy of insecticides used in mosquito control. mosquito breeding sites vary from opened to high vegetation covered areas leading to a large ultraviolet gradient exposure ...201323911355
efficacy of various larvicides against aedes aegypti immatures in the laboratory.we conducted a laboratory study to evaluate the efficacy of control agents against small larvae, large larvae, and pupae of aedes aegypti to determine an appropriate larvicide regime to employ in emergency dengue control programs. the control agents included bacillus thuringiensis var. israelensis (bti), pyriproxyfen (an insect growth regulator), a larvicidal oil, aquatain amf (polydimethylsiloxane, a monomolecular film), and temephos at the recommend application dosages and rates. our results s ...201323883850
increase in larval gut proteolytic activities and bti resistance in the dengue fever mosquito.the bioinsecticide bacillus thuringiensis var. israelensis (bti) is increasingly used worldwide for mosquito control. although no established resistance to bti has been described in the field so far, a resistant aedes aegypti strain (litox strain) was selected in the laboratory using field-collected leaf litter containing bti toxins. this selected strain exhibits a moderate resistance level to bti, but a high resistance level to individual cry toxins. as bti contains four different toxins, gener ...201323192850
effect of wolbachia on insecticide susceptibility in lines of aedes aegypti.two stable infections of wolbachia pipientis, wmelpop and wmel, now established in aedes aegypti, are being used in a biocontrol program to suppress the transmission of dengue. any effects of wolbachia infection on insecticide resistance of mosquitoes may undermine the success of this program. bioassays of ae. aegypti were conducted to test for differences in response to insecticides between wolbachia infected (wmelpop, wmel) and uninfected lines. insecticides screened were bifenthrin, the pyret ...201323149015
spray application of bacillus thuringiensis israelensis (bti strain am65-52) against aedes aegypti (l.) and ae. albopictus skuse populations and impact on dengue transmission in a dengue endemic residential site in malaysia.a one year study was conducted to evaluate the impact of spray application of bacillus thuringiensis israelensis (bti), strain am65-52 on vector populations and dengue transmission in a dengue endemic state in malaysia. residential sites with similar populations of aedes aegypti (l.) and aedes albopictus skuse were studied. one site was treated with spray application of bti into all outdoor target vector habitats, which consisted of natural and artificial containers. the other site was not treat ...201223082582
investigation of the cry4b-prohibitin interaction in aedes aegypti cells.bacillus thuringiensis (bt) produces insecticidal toxins active against insects. cry4b, one of the major insecticidal toxins produced by bt subsp. israelensis, is highly toxic to mosquitoes in the genus aedes: the major vectors of dengue, yellow fever, and chikungunya. previous work has shown that cry4b binds to several mid-gut membrane proteins in aedes aegypti larvae including prohibitin, a protein recently identified as a receptor that also mediates entry of dengue virus into aedes cells. thi ...201222767320
isolation of bacillus thuringiensis strains that contain dipteran-specific cry genes from ilha bela (são paulo, brazil) soil samples.the entomophatogenic bacterium bacillus thuringiensis produces crystal proteins, named cry proteins which are encoded by the cry genes. this bacterium is used on biological control of important economical pests, as well as in the control of disease´s vectors, such as aedes aegypti, a mosquito that transmits the dengue viruses. isolates of this bacterium can be characterized by the content of cry genes and this prediction helps target different insect orders. in this research, we isolated 76 colo ...201222735130
efficacy of sunlight-activatable porphyrin formulates on larvae of anopheles gambiae m and s molecular forms and an. arabiensis: a potential novel biolarvicide for integrated malaria vector control.biolarvicides, such as microbial formulations based on bacillus thuringiensis and b. sphaericus, have been found to be highly effective against mosquito larvae and are currently employed as eco-friendly alternatives to synthetic chemical insecticides for vector control. recently, a porphyrin of natural origin has been suggested as a sunlight-activatable larvicide against the dengue vector aedes aegypti. in order to validate the approach for the control of the malaria vector, we tested the photo- ...201222668835
identifying insecticide resistance genes in mosquito by combining aflp genome scans and 454 pyrosequencing.aflp-based genome scans are widely used to study the genetics of adaptation and to identify genomic regions potentially under selection. however, this approach usually fails to detect the actual genes or mutations targeted by selection owing to the difficulties of obtaining dna sequences from aflp fragments. here, we combine classical aflp outlier detection with 454 sequencing of aflp fragments to obtain sequences from outlier loci. we applied this approach to the study of resistance to bacillus ...201222348648
arboviral diseases and malaria in australia, 2011-12: annual report of the national arbovirus and malaria advisory committee.the national notifiable diseases surveillance system received notifications for 7,875 cases of disease transmitted by mosquitoes during the 2011-12 season (1 july 2011 to 30 june 2012). the alphaviruses barmah forest virus and ross river virus accounted for 6,036 (77%) of these. there were 18 notifications of dengue virus infection acquired in australia and 1,390 cases that were acquired overseas, while for 38 cases, the place of acquisition was unknown. imported cases of dengue in australia wer ...201425222207
myco-synthesis of silver nanoparticles using beauveria bassiana against dengue vector, aedes aegypti (diptera: culicidae).the efficacy of silver synthesized biolarvicide with the help of entomopathogenic fungus, beauveria bassiana, was assessed against the different larval instars of dengue vector, aedes aegypti. the silver nanoparticles were observed and characterized by a scanning electron microscope (sem) and energy-dispersive x-ray (edx). a surface plasmon resonance band was observed at 420 nm in uv-vis spectrophotometer. the characterization was confirmed by shape (spherical), size 36.88-60.93 nm, and edx spec ...201424861012
aedes-borne virus-mosquito interactions: mass spectrometry strategies and findings.aedes-borne viruses are responsible for high-impact neglected tropical diseases and unpredictable outbreaks such as the ongoing zika epidemics. aedes mosquitoes spread different arboviruses such as dengue virus (denv), chikungunya virus (chikv), and zika virus, among others, and are responsible for the continuous emergence and reemergence of these pathogens. these viruses have complex transmission cycles that include two hosts, namely the aedes mosquito as a vector and susceptible vertebrate hos ...201728192064
environmental and social change drive the explosive emergence of zika virus in the americas.since zika virus (zikv) was detected in brazil in 2015, it has spread explosively across the americas and has been linked to increased incidence of microcephaly and guillain-barré syndrome (gbs). in one year, it has infected over 500,000 people (suspected and confirmed cases) in 40 countries and territories in the americas. along with recent epidemics of dengue (denv) and chikungunya virus (chikv), which are also transmitted by aedes aegypti and ae. albopictus mosquitoes, the emergence of zikv s ...201728182667
transcriptome analysis of aedes aegypti in response to mono-infections and co-infections of dengue virus-2 and chikungunya virus.chikungunya virus (chikv) and dengue virus (denv) spread via the bite of infected aedes mosquitoes. both these viruses exist as co-infections in the host as well as the vector and are known to exploit their cellular machinery for their replication. while there are studies reporting the changes in aedes transcriptome when infected with denv and chikv individually, the effect both these viruses have on the mosquitoes when present as co-infections is not clearly understood. in the present study, we ...201728161634
engineered aedes aegypti jak/stat pathway-mediated immunity to dengue virus.we have developed genetically modified ae. aegypti mosquitoes that activate the conserved antiviral jak/stat pathway in the fat body tissue, by overexpressing either the receptor dome or the janus kinase hop by the blood feeding-induced vitellogenin (vg) promoter. transgene expression inhibits infection with several dengue virus (denv) serotypes in the midgut as well as systemically and in the salivary glands. the impact of the transgenes dome and hop on mosquito longevity was minimal, but it re ...201728081143
identification and initial characterization of matrix metalloproteinases in the yellow fever mosquito, aedes aegypti.aedes aegypti is a major vector for arboviruses such as dengue, chikungunya and zika viruses. during acquisition of a viremic bloodmeal, an arbovirus infects mosquito midgut cells before disseminating to secondary tissues, including the salivary glands. once virus is released into the salivary ducts it can be transmitted to another vertebrate host. the midgut is surrounded by a basal lamina (bl) in the extracellular matrix, consisting of a proteinaceous mesh composed of collagen iv and laminin. ...201728054419
impact of autocidal gravid ovitraps on chikungunya virus incidence in aedes aegypti (diptera: culicidae) in areas with and without traps.puerto rico detected the first confirmed case of chikungunya virus (chikv) in may 2014 and the virus rapidly spread throughout the island. the invasion of chikv allowed us to observe aedes aegypti (l.) densities, infection rates, and impact of vector control in urban areas using cdc autocidal gravid ovitraps (ago traps) for mosquito control over several years. because local mosquitoes can only get the virus from infectious residents, detecting the presence of virus in mosquitoes functions as a p ...201628031347
indoor development of aedes aegypti in germany, 2016.in spring 2016, a german traveller returning from martinique cultivated imported plant offsets in her home, and accidentally bred aedes aegypti. thirteen adult mosquito specimens submitted for identification and the traveller were tested for zika, dengue and chikungunya virus infections, with negative results. the detection of ae. aegypti by the 'mueckenatlas' project demonstrates the value of this passive surveillance scheme for potential public health threats posed by invasive mosquitoes in ge ...201627918261
[recommendations for the surveillance of aedes aegypti].diseases caused by arboviruses transmitted by aedes aegypti, such as dengue, chikungunya and zika, continue to rise in annual incidence and geographic expansion. a key limitation for achieving control of a. aegypti has been the lack of effective tools for monitoring its population, and thus determine what control measures actually work. surveillance of a. aegypti has been based mainly on immature indexes, but they bear little relation to the number of mosquito females, which are the ones capable ...201627869394
testing of visual and chemical attractants in correlation with the development and field evaluation of an autodissemination station for the suppression of aedes aegypti and aedes albopictus in florida.three exotic mosquito-borne pathogens-dengue, chikungunya, and zika viruses-transmitted by aedes albopictus and ae. aegypti have undergone dramatic global expansion in recent years. the control of vector populations and minimizing bites from these vectors are the primary methods of reducing risk of transmission of these viruses to humans. however, ae. albopictus and ae. aegypti are notoriously challenging to control through conventional chemical means, due primarily to difficulties in applying p ...201627802398
knockdown resistance mutations in aedes aegypti (diptera: culicidae) from puerto rico.permethrin resistance is widespread in aedes aegypti (l.), the main dengue, zika, and chikungunya virus vector in latin america and the caribbean. a common mechanism of resistance to pyrethroids-knockdown resistance (kdr)-is conferred through mutations in the insect's voltage-dependent sodium channel. in this mosquito, around 10 replacement substitutions in the voltage-gated sodium channel gene (vgsc) have been reported in pyrethroid-resistant strains. two of these mutations, named ile1,016 and ...201627493252
distribution of aedes mosquitoes in the kilimanjaro region of northern tanzania.little is known about the presence and distribution of aedes mosquitoes in northern tanzania despite the occurence of viruses transmitted by these mosquitoes such as chikungunya virus (chikv) and dengue virus (denv) in the region. adult and larval mosquitoes were collected from rural and urban settings across a wide range of altitudes in the kilimanjaro region using the mosquito magnet co2 trap for collection of adults and old tires for breeding of larvae. polymerase chain reaction assays were p ...201627376502
evidence for natural vertical transmission of chikungunya viruses in field populations of aedes aegypti in delhi and haryana states in india-a preliminary report.aedes aegypti and aedes albopictus are principal vectors for the transmission of chikungunya virus (chikv). india is a hub for both dengue and chikungunya infections and there are several reports of co-infection of dengue and chikungunya virus in the clinical scenario. the present pilot entomological survey was conducted to evaluate vertical transmission of chikv in aedes field populations. aedes immature (larvae and pupae) collection was done in 2012, over a period of six months from selected s ...201627282096
reduced incidence of chikungunya virus infection in communities with ongoing aedes aegypti mosquito trap intervention studies - salinas and guayama, puerto rico, november 2015-february 2016.aedes species mosquitoes transmit chikungunya virus, as well as dengue and zika viruses, and bite most often during the day.* infectious mosquito bites frequently occur in and around homes (1,2). caribbean countries first reported local transmission of chikungunya virus in december 2013, and soon after, chikungunya virus spread throughout the americas (3). puerto rico reported its first laboratory-positive chikungunya case in may 2014 (4), and subsequently identified approximately 29,000 suspect ...201627171600
the zika outbreak of the 21st century.the zika virus outbreak has captivated the attention of the global audience and information has spread rapidly and wildly through the internet and other media channels. this virus was first identified in 1947, when it was isolated from a sentinel rhesus monkey placed by british scientists working at the yellow fever research laboratory located in the zika forest area of uganda, hence its name, and is transmitted primarily by the mosquito vector, aedes aegypti. the fact that the rhesus macaque is ...201626925496
evidence of vertical transmission and co-circulation of chikungunya and dengue viruses in field populations of aedes aegypti (l.) from guerrero, mexico.we report results of the entomo-virological surveillance system in aedes aegypti local populations performed by the ministry of health of guerrero.201626711697
a mosquito survey of the twin-island caribbean nation of saint kitts and nevis, 2010.adult mosquito surveys of saint kitts and nevis (skn) were performed in the dry season (march 16-23, 2010) in saint kitts, and the rainy season (october 18-25, 2010) in skn. biogents (bg) sentinel traps were set with co₂and bg lure in urban, rural, mangrove, and dry forest habitats. mosquitoes were identified to species, and reverse transcription-polymerase chain reaction was performed on potential vector species for dengue virus (denv), chikungunya virus (chikv), and west nile virus (wnv). the ...201526675458
historical inability to control aedes aegypti as a main contributor of fast dispersal of chikungunya outbreaks in latin america.the arrival of chikungunya fever (chikf) in latin american countries has been expected to trigger epidemics and challenge health systems. historically considered as dengue-endemic countries, abundant aedes aegypti populations make this region highly vulnerable to chikungunya virus (chikv) circulation. this review describes the current dengue and chikf epidemiological situations, as well as the role of uncontrolled ae. aegypti and aedes albopictus vectors in spreading the emerging chikv. comments ...201526518229
arbovirus surveillance and first report of chikungunya virus in wild populations of aedes aegypti from guerrero, mexico.we carried out dengue (denv) and chikungunya virus (chikv) surveillance in wild populations of aedes aegypti from guerrero, mexico, from 2012 to 2014 following a standard national protocol of the mexican dengue control program. a total of 284 pools (15-30 specimens/pool) of female mosquitoes were tested with real-time reverse transcriptase-polymerase chain reaction to detect denv and chikv. we report for the 1st time the detection of chikv from field-collected mosquitoes at acapulco and juchitán ...201526375910
assessing carbon dioxide and synthetic lure-baited traps for dengue and chikungunya vector surveillance (3).the aedes mosquito vectors of dengue virus (denv) and chikungunya virus (chikv) are attracted to specific host cues that are not generated by traditional light traps. for this reason multiple companies have designed traps to specifically target those species. recently the standard trap for denv and chikv vectors, the bg-sentinel (bgs) trap, has been remodeled to be more durable and better suited for use in harsh field conditions, common during military operations, and relabeled the bg-sentinel 2 ...201526375905
[lessons learned in the control of aedes aegypti to address dengue and the emergency of chikungunya in iquitos, peru].dengue has affected iquitos since 1990 causing outbreaks of major impact on public health and for this reason great efforts have been made for its temporal control. currently, with the expansion of the chikungunya virus in the americas and the threat of the emergence of the virus in iquitos, we reflect on lessons learned by way of the activities undertaken in the area of vector control; epidemiological surveillance, diagnosis and clinical management during periods of outbreaks of dengue, in a wa ...201526102121
[chikungunya, an emerging viral disease. proposal of an algorithm for its clinical management].chikungunya fever (chik) is an emerging viral disease. it is caused by the chikungunya virus, an alphavirus from the togaviridae family. it is transmitted to humans by the bite of infected mosquitoes, mainly aedes aegypti and aedes albopictus. they are also involved in the transmission of dengue, malaria, etc. chik is now endemic in any region of africa and southeast-asia. cases of chik have been reported in america, the caribbean, and europe (france, italy and spain). there are reservoirs of th ...201725440971
feeding host range of aedes albopictus (diptera: culicidae) demonstrates its opportunistic host-seeking behavior in rural singapore.aedes albopictus (skuse) is a competent vector of arboviruses of public health importance, including dengue virus (denv) and chikungunya virus viruses. ae. albopictus is the primary vector of chikungunya virus in singapore. however, despite being ubiquitous, it plays a secondary role in denv transmission. the vectorial capacity of ae. albopictus for denv in field settings appears to be weak because dengue primarily occurs in aedes aegypti (l.)-dominated, urban settings of the country. as host-se ...201425118424
the impact of temperature on the bionomics of aedes (stegomyia) aegypti, with special reference to the cool geographic range margins.the mosquito aedes (stegomyia) aegypti (l.), which occurs widely in the subtropics and tropics, is the primary urban vector of dengue and yellow fever viruses, and an important vector of chikungunya virus. there is substantial interest in how climate change may impact the bionomics and pathogen transmission potential of this mosquito. this forum article focuses specifically on the effects of temperature on the bionomics of ae. aegypti, with special emphasis on the cool geographic range margins w ...201424897844
chikungunya and dengue autochthonous cases in europe, 2007-2012.a large number of autochthonous cases of dengue fever (2237) and chikungunya fever (231) occurred in europe (italy, france, croatia, madeira) during the period covered by our analysis (2007-2012). in all dengue outbreaks, the circulating strain, identified by means of molecular analysis, was the denv-1 strain. dengue and chikungunya are infectious diseases that often result in hospitalizations and are associated with high public health costs. the dengue epidemic on the island of madeira resulted ...201423962447
blood meal induced microrna regulates development and immune associated genes in the dengue mosquito vector, aedes aegypti.aedes aegypti is a blood-feeding mosquito that transmits human pathogens such as dengue virus, yellow fever virus and chikungunya virus. recently, dramatic changes in the transcriptome of ae. aegypti following a blood meal have been reported; however, the molecular factors involved in regulating these changes are largely unknown. in this study, we found induction of a number of endogenous micrornas (mirnas) in blood fed (bf) mosquitoes. one of these mirnas, aae-mir-375, was only detected in bf m ...201323202267
chikungunya virus infection, a threat to public health.many countries in the world have reported human infections by chikungunya virus (chikv) a mosquito-borne togavirus belonging to the genus alpha virus. after half a century of focal out breaks of acute febrile poly arthralgia, the disease had spread unexpectedly in the past decade with large outbreaks in africa around the indian ocean and in bangladesh. in asia, chikv is thought to be transmitted by the same mosquito as dengue, aedes aegypti and aedes albopictus. due to similarities in clinical p ...201222561790
detection of chikungunya virus in aedes aegypti during 2011 outbreak in al hodayda, yemen.in october 2010, the ministry of public health and population reported an outbreak of dengue-like acute febrile illness in al hodayda governorate. by january 2011, a total of 1542 cases had been recorded from 19 of the 26 districts in the governorate with 104 purportedly associated deaths. in response this event, in january 2011 entomological investigations aimed at identifying the primary vector and the epidemic associated etiological agent were carried out. based on the reported cases and the ...201222469818
optimal control problems of mosquito-borne disease subject to changes in feeding behavior of aedes mosquitoes.dengue viruses (denv) are transmitted to humans by the bite of aedes mosquitoes. it is known that dengue virus infection in aedes aegypti female mosquitoes makes a change in the feeding behavior of the infected mosquitoes. in this study, using the forces of infection, we incorporated the effect of changes in the feeding behavior of mosquitoes into the standard vector-borne sir-si model. it has been proved that both a single-strain model and a two-strain model exhibit forward bifurcations. moreov ...201728385591
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